pax_global_header00006660000000000000000000000064124637151410014516gustar00rootroot0000000000000052 comment=352908143497da0640b928248165e83212dc4298 bcftools-1.2/000077500000000000000000000000001246371514100131735ustar00rootroot00000000000000bcftools-1.2/AUTHORS000066400000000000000000000006051246371514100142440ustar00rootroot00000000000000BCFtools package is currently maintained by Petr Danecek, Shane McCarthy and John Marshall. Alphabetical list of people who have made contributions: Nicholas Clarke Petr Danecek Warren Kretzschmar Heng Li Shane McCarthy John Marshall Joel Martin Stephan Schiffels bcftools-1.2/HMM.c000066400000000000000000000272151246371514100137670ustar00rootroot00000000000000/* The MIT License Copyright (c) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include "HMM.h" static inline void multiply_matrix(int n, double *a, double *b, double *dst, double *tmp) { double *out = dst; if ( a==dst || b==dst ) out = tmp; int i,j,k; for (i=0; instates = nstates; hmm->curr_tprob = (double*) malloc(sizeof(double)*nstates*nstates); hmm->tmp = (double*) malloc(sizeof(double)*nstates*nstates); hmm_set_tprob(hmm, tprob, ntprob); return hmm; } void hmm_set_tprob(hmm_t *hmm, double *tprob, int ntprob) { hmm->ntprob_arr = ntprob; if ( ntprob<=0 ) ntprob = 1; if ( !hmm->tprob_arr ) hmm->tprob_arr = (double*) malloc(sizeof(double)*hmm->nstates*hmm->nstates*ntprob); memcpy(hmm->tprob_arr,tprob,sizeof(double)*hmm->nstates*hmm->nstates); int i; for (i=1; instates, hmm->tprob_arr, hmm->tprob_arr+(i-1)*hmm->nstates*hmm->nstates, hmm->tprob_arr+i*hmm->nstates*hmm->nstates, hmm->tmp); } void hmm_set_tprob_func(hmm_t *hmm, set_tprob_f set_tprob, void *data) { hmm->set_tprob = set_tprob; hmm->set_tprob_data = data; } static void _set_tprob(hmm_t *hmm, int pos_diff) { assert( pos_diff>=0 ); int i, n; n = hmm->ntprob_arr ? pos_diff % hmm->ntprob_arr : 0; // n-th precalculated matrix memcpy(hmm->curr_tprob, hmm->tprob_arr+n*hmm->nstates*hmm->nstates, sizeof(*hmm->curr_tprob)*hmm->nstates*hmm->nstates); if ( hmm->ntprob_arr > 0 ) { n = pos_diff / hmm->ntprob_arr; // number of full blocks to jump for (i=0; instates, hmm->tprob_arr+(hmm->ntprob_arr-1)*hmm->nstates*hmm->nstates, hmm->curr_tprob, hmm->curr_tprob, hmm->tmp); } } void hmm_run_viterbi(hmm_t *hmm, int n, double *eprobs, uint32_t *sites) { // Init arrays when run for the first time if ( hmm->nvpath < n ) { hmm->nvpath = n; hmm->vpath = (uint8_t*) realloc(hmm->vpath, sizeof(uint8_t)*hmm->nvpath*hmm->nstates); } if ( !hmm->vprob ) { hmm->vprob = (double*) malloc(sizeof(double)*hmm->nstates); hmm->vprob_tmp = (double*) malloc(sizeof(double)*hmm->nstates); } // Init all states with equal likelihood int i,j, nstates = hmm->nstates; for (i=0; ivprob[i] = 1./nstates; // Run Viterbi uint32_t prev_pos = sites[0]; for (i=0; ivpath[i*nstates]; double *eprob = &eprobs[i*nstates]; int pos_diff = sites[i] == prev_pos ? 0 : sites[i] - prev_pos - 1; _set_tprob(hmm, pos_diff); if ( hmm->set_tprob ) hmm->set_tprob(hmm, prev_pos, sites[i], hmm->set_tprob_data); prev_pos = sites[i]; double vnorm = 0; for (j=0; jvprob[k] * MAT(hmm->curr_tprob,hmm->nstates,j,k); if ( vmax < pval ) { vmax = pval; k_vmax = k; } } vpath[j] = k_vmax; hmm->vprob_tmp[j] = vmax * eprob[j]; vnorm += hmm->vprob_tmp[j]; } for (j=0; jvprob_tmp[j] /= vnorm; double *tmp = hmm->vprob; hmm->vprob = hmm->vprob_tmp; hmm->vprob_tmp = tmp; } // Find the most likely state int iptr = 0; for (i=1; ivprob[iptr] < hmm->vprob[i] ) iptr = i; // Trace back the Viterbi path, we are reusing vpath for storing the states (vpath[i*nstates]) for (i=n-1; i>=0; i--) { assert( iptrvpath[i*nstates + iptr]vpath[i*nstates + iptr]; hmm->vpath[i*nstates] = iptr; // reusing the array for different purpose here } } void hmm_run_fwd_bwd(hmm_t *hmm, int n, double *eprobs, uint32_t *sites) { // Init arrays when run for the first time if ( hmm->nfwd < n ) { hmm->nfwd = n; hmm->fwd = (double*) realloc(hmm->fwd, sizeof(double)*(hmm->nfwd+1)*hmm->nstates); } if ( !hmm->bwd ) { hmm->bwd = (double*) malloc(sizeof(double)*hmm->nstates); hmm->bwd_tmp = (double*) malloc(sizeof(double)*hmm->nstates); } // Init all states with equal likelihood int i,j,k, nstates = hmm->nstates; for (i=0; ifwd[i] = 1./hmm->nstates; for (i=0; ibwd[i] = 1./hmm->nstates; // Run fwd uint32_t prev_pos = sites[0]; for (i=0; ifwd[i*nstates]; double *fwd = &hmm->fwd[(i+1)*nstates]; double *eprob = &eprobs[i*nstates]; int pos_diff = sites[i] == prev_pos ? 0 : sites[i] - prev_pos - 1; _set_tprob(hmm, pos_diff); if ( hmm->set_tprob ) hmm->set_tprob(hmm, prev_pos, sites[i], hmm->set_tprob_data); prev_pos = sites[i]; double norm = 0; for (j=0; jcurr_tprob,hmm->nstates,j,k); fwd[j] = pval * eprob[j]; norm += fwd[j]; } for (j=0; jbwd, *bwd_tmp = hmm->bwd_tmp; prev_pos = sites[n-1]; for (i=0; ifwd[(n-i)*nstates]; double *eprob = &eprobs[(n-i-1)*nstates]; int pos_diff = sites[n-i-1] == prev_pos ? 0 : prev_pos - sites[n-i-1] - 1; _set_tprob(hmm, pos_diff); if ( hmm->set_tprob ) hmm->set_tprob(hmm, sites[n-i-1], prev_pos, hmm->set_tprob_data); prev_pos = sites[n-i-1]; double bwd_norm = 0; for (j=0; jcurr_tprob,hmm->nstates,k,j); bwd_tmp[j] = pval; bwd_norm += pval; } double norm = 0; for (j=0; jnfwd < n ) { hmm->nfwd = n; hmm->fwd = (double*) realloc(hmm->fwd, sizeof(double)*(hmm->nfwd+1)*hmm->nstates); } if ( !hmm->bwd ) { hmm->bwd = (double*) malloc(sizeof(double)*hmm->nstates); hmm->bwd_tmp = (double*) malloc(sizeof(double)*hmm->nstates); } // Init all states with equal likelihood int i,j,k, nstates = hmm->nstates; for (i=0; ifwd[i] = 1./hmm->nstates; for (i=0; ibwd[i] = 1./hmm->nstates; // New transition matrix: temporary values double *tmp_xi = (double*) calloc(nstates*nstates,sizeof(double)); double *tmp_gamma = (double*) calloc(nstates,sizeof(double)); double *fwd_bwd = (double*) malloc(sizeof(double)*nstates); // Run fwd uint32_t prev_pos = sites[0]; for (i=0; ifwd[i*nstates]; double *fwd = &hmm->fwd[(i+1)*nstates]; double *eprob = &eprobs[i*nstates]; int pos_diff = sites[i] == prev_pos ? 0 : sites[i] - prev_pos - 1; _set_tprob(hmm, pos_diff); if ( hmm->set_tprob ) hmm->set_tprob(hmm, prev_pos, sites[i], hmm->set_tprob_data); prev_pos = sites[i]; double norm = 0; for (j=0; jcurr_tprob,hmm->nstates,j,k); fwd[j] = pval * eprob[j]; norm += fwd[j]; } for (j=0; jbwd, *bwd_tmp = hmm->bwd_tmp; prev_pos = sites[n-1]; for (i=0; ifwd[(n-i)*nstates]; double *eprob = &eprobs[(n-i-1)*nstates]; int pos_diff = sites[n-i-1] == prev_pos ? 0 : prev_pos - sites[n-i-1] - 1; _set_tprob(hmm, pos_diff); if ( hmm->set_tprob ) hmm->set_tprob(hmm, sites[n-i-1], prev_pos, hmm->set_tprob_data); prev_pos = sites[n-i-1]; double bwd_norm = 0; for (j=0; jcurr_tprob,hmm->nstates,k,j); bwd_tmp[j] = pval; bwd_norm += pval; } double norm = 0; for (j=0; jtprob_arr,hmm->nstates,k,j)*eprob[k] / norm; } } for (j=0; jcurr_tprob,nstates,k,j) = MAT(tmp_xi,nstates,k,j) / tmp_gamma[j]; norm += MAT(hmm->curr_tprob,nstates,k,j); } for (k=0; kcurr_tprob,nstates,k,j) /= norm; } free(tmp_gamma); free(tmp_xi); free(fwd_bwd); } void hmm_destroy(hmm_t *hmm) { free(hmm->vprob); free(hmm->vprob_tmp); free(hmm->vpath); free(hmm->curr_tprob); free(hmm->tmp); free(hmm->tprob_arr); free(hmm->fwd); free(hmm->bwd); free(hmm->bwd_tmp); free(hmm); } bcftools-1.2/HMM.h000066400000000000000000000106331246371514100137700ustar00rootroot00000000000000/* The MIT License Copyright (c) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #ifndef __HMM_H__ #define __HMM_H__ #define MAT(matrix,ndim,i,j) (matrix)[(ndim)*(i)+(j)] // P(i|j), that is, transition j->i typedef struct _hmm_t hmm_t; typedef void (*set_tprob_f) (hmm_t *hmm, uint32_t prev_pos, uint32_t pos, void *data); struct _hmm_t { int nstates; // number of states double *vprob, *vprob_tmp; // viterbi probs [nstates] uint8_t *vpath; // viterbi path [nstates*nvpath] double *bwd, *bwd_tmp; // bwd probs [nstates] double *fwd; // fwd probs [nstates*(nfwd+1)] int nvpath, nfwd; int ntprob_arr; // number of pre-calculated tprob matrices double *curr_tprob, *tmp; // Temporary arrays; curr_tprob is short lived, valid only for // one site (that is, one step of Viterbi algorithm) double *tprob_arr; // Array of transition matrices, precalculated to ntprob_arr // positions. The first matrix is the initial tprob matrix // set by hmm_init() or hmm_set_tprob() set_tprob_f set_tprob; // Optional user function to set / modify transition probabilities // at each site (one step of Viterbi algorithm) void *set_tprob_data; }; /** * hmm_init() - initialize HMM * @nstates: number of states * @tprob: transition probabilities matrix (nstates x nstates), for elements ordering * see the MAT macro above. * @ntprob: number of precalculated tprob matrices or 0 for constant probs, independent * of distance */ hmm_t *hmm_init(int nstates, double *tprob, int ntprob); void hmm_set_tprob(hmm_t *hmm, double *tprob, int ntprob); /** * hmm_set_tprob_func() - custom setter of transition probabilities */ void hmm_set_tprob_func(hmm_t *hmm, set_tprob_f set_tprob, void *data); /** * hmm_run_viterbi() - run Viterbi algorithm * @nsites: number of sites * @eprob: emission probabilities for each site and state (nsites x nstates) * @sites: list of positions * * When done, hmm->vpath[] contains the calculated Viterbi path. The states * are indexed starting from 0, a state at i-th site can be accessed as * vpath[nstates*i]. */ void hmm_run_viterbi(hmm_t *hmm, int nsites, double *eprob, uint32_t *sites); /** * hmm_run_fwd_bwd() - run the forward-backward algorithm * @nsites: number of sites * @eprob: emission probabilities for each site and state (nsites x nstates) * @sites: list of positions * * When done, hmm->fwd[] contains the calculated fwd*bwd probabilities. The * probability of i-th state at j-th site can be accessed as fwd[j*nstates+i]. */ void hmm_run_fwd_bwd(hmm_t *hmm, int nsites, double *eprob, uint32_t *sites); /** * hmm_run_baum_welch() - run one iteration of Baum-Welch algorithm * @nsites: number of sites * @eprob: emission probabilities for each site and state (nsites x nstates) * @sites: list of positions * * Same as hmm_run_fwd_bwd, in addition curr_tprob contains the new * transition probabilities. In this verison, emission probabilities * are not updated. */ void hmm_run_baum_welch(hmm_t *hmm, int nsites, double *eprob, uint32_t *sites); void hmm_destroy(hmm_t *hmm); #endif bcftools-1.2/INSTALL000066400000000000000000000037051246371514100142310ustar00rootroot00000000000000System Requirements =================== BCFtools and HTSlib depend on the zlib library . Building them requires zlib development files to be installed on the build machine; you may need to ensure a package such as zlib1g-dev (on Debian or Ubuntu Linux) or zlib-devel (on RPM/yum-based distributions) is installed. Compilation =========== 'cd' to the bcftools-1.x directory containing the package's source and type 'make' to compile BCFtools. This BCFtools release contains a copy of HTSlib which will be used to build BCFtools. If you already have a system-installed HTSlib or another HTSlib that you would prefer to build against, you can arrange this by overriding $(HTSDIR) by typing 'make HTSDIR=/path/to/htslib-source' -- see the makefile for details. Optional Compilation with GSL ============================= The 'polysomy' command depends on the GNU Scientific Library (GSL) and is not enabled by default. In order to compile it, type 'make clean && make USE_GPL=1'. Note that GSL is distributed under a GPL license, so when USE_GPL=1 is used to compile bcftools, the resulting program must only be distributed under terms compatible with that license. In the default compilation mode the program is dual licensed and you may choose to be licensed under the terms of the MIT/Expat license or the GNU General Public License (GPL). Installation ============ Type 'make install' to install the bcftools executable and associated scripts and a manual page to /usr/local. Type 'make prefix=/path/to/dir install' to install everything under your choice of installation directory. The install target also understands DESTDIR and the other usual installation directory variables. The bgzip and tabix utilities are provided by HTSlib. If you have not also installed HTSlib separately, you may wish to install these utilities by hand by copying bcftools-1.x/htslib-1.x/{bgzip,tabix} to the same bin directory to which you have installed bcftools et al. bcftools-1.2/LICENSE000066400000000000000000001111021246371514100141740ustar00rootroot00000000000000This software is available to you under a choice of one of two licenses. You may chose to be licensed under the terms of the MIT/Expat license or the GNU General Public License (GPL), both included below. If compiled with the GNU Scientific Library (which is optional and disabled by default as explained in the INSTALL document), the use of this software is governed by the GPL license. ----------------------------------------------------------------------------- The MIT/Expat License Copyright (C) 2012-2014 Genome Research Ltd. Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. 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But first, please read . ----------------------------------------------------------------------------- bcftools-1.2/Makefile000066400000000000000000000176741246371514100146520ustar00rootroot00000000000000# Makefile for bcftools, utilities for Variant Call Format VCF/BCF files. # # Copyright (C) 2012-2014 Genome Research Ltd. # # Author: Petr Danecek # # Permission is hereby granted, free of charge, to any person obtaining a copy # of this software and associated documentation files (the "Software"), to deal # in the Software without restriction, including without limitation the rights # to use, copy, modify, merge, publish, distribute, sublicense, and/or sell # copies of the Software, and to permit persons to whom the Software is # furnished to do so, subject to the following conditions: # # The above copyright notice and this permission notice shall be included in # all copies or substantial portions of the Software. # # THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR # IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, # FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL # THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER # LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING # FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER # DEALINGS IN THE SOFTWARE. PROG= bcftools TEST_PROG= test/test-rbuf all: $(PROG) $(TEST_PROG) # Adjust $(HTSDIR) to point to your top-level htslib directory HTSDIR = ../htslib include $(HTSDIR)/htslib.mk HTSLIB = $(HTSDIR)/libhts.a BGZIP = $(HTSDIR)/bgzip TABIX = $(HTSDIR)/tabix CC = gcc CFLAGS = -g -Wall -Wc++-compat -O2 DFLAGS = OBJS = main.o vcfindex.o tabix.o \ vcfstats.o vcfisec.o vcfmerge.o vcfquery.o vcffilter.o filter.o vcfsom.o \ vcfnorm.o vcfgtcheck.o vcfview.o vcfannotate.o vcfroh.o vcfconcat.o \ vcfcall.o mcall.o vcmp.o gvcf.o reheader.o convert.o vcfconvert.o tsv2vcf.o \ vcfcnv.o HMM.o vcfplugin.o consensus.o ploidy.o version.o \ ccall.o em.o prob1.o kmin.o # the original samtools calling INCLUDES = -I. -I$(HTSDIR) # The polysomy command is not compiled by default because it brings dependency # on libgsl. The command can be compiled wth `make USE_GPL=1`. See the INSTALL # and LICENSE documents to understand license implications. ifdef USE_GPL CFLAGS += -DUSE_GPL OBJS += polysomy.o LDLIBS = -lgsl -lcblas endif prefix = /usr/local exec_prefix = $(prefix) bindir = $(exec_prefix)/bin mandir = $(prefix)/share/man man1dir = $(mandir)/man1 MKDIR_P = mkdir -p INSTALL = install -p INSTALL_PROGRAM = $(INSTALL) INSTALL_DATA = $(INSTALL) -m 644 INSTALL_DIR = $(MKDIR_P) -m 755 all:$(PROG) plugins # See htslib/Makefile PACKAGE_VERSION = 1.2 ifneq "$(wildcard .git)" "" PACKAGE_VERSION := $(shell git describe --always --dirty) DOC_VERSION := $(shell git describe --always)+ DOC_DATE := $(shell date +'%Y-%m-%d %R %Z') version.h: $(if $(wildcard version.h),$(if $(findstring "$(PACKAGE_VERSION)",$(shell cat version.h)),,force)) endif version.h: echo '#define BCFTOOLS_VERSION "$(PACKAGE_VERSION)"' > $@ .SUFFIXES:.c .o .PHONY:all clean clean-all clean-plugins distclean install lib tags test testclean force plugins docs force: .c.o: $(CC) -c $(CFLAGS) $(DFLAGS) $(INCLUDES) $< -o $@ test: $(PROG) plugins test/test-rbuf $(BGZIP) $(TABIX) ./test/test.pl --exec bgzip=$(BGZIP) --exec tabix=$(TABIX) test-plugins: $(PROG) plugins test/test-rbuf $(BGZIP) $(TABIX) ./test/test.pl --plugins --exec bgzip=$(BGZIP) --exec tabix=$(TABIX) # Plugin rules PLUGINC = $(foreach dir, plugins, $(wildcard $(dir)/*.c)) PLUGINS = $(PLUGINC:.c=.so) PLUGINM = $(PLUGINC:.c=.mk) %.so: %.c version.h version.c $(HTSDIR)/libhts.so $(CC) $(CFLAGS) $(INCLUDES) -fPIC -shared -o $@ version.c $< -L$(HTSDIR) -lhts -include $(PLUGINM) plugins: $(PLUGINS) bcftools_h = bcftools.h $(htslib_vcf_h) call_h = call.h $(htslib_vcf_h) $(htslib_synced_bcf_reader_h) vcmp.h convert_h = convert.h $(htslib_vcf_h) tsv2vcf_h = tsv2vcf.h $(htslib_vcf_h) filter_h = filter.h $(htslib_vcf_h) prob1_h = prob1.h $(htslib_vcf_h) $(call_h) roh_h = HMM.h $(htslib_vcf_h) $(htslib_synced_bcf_reader_h) $(HTSDIR)/htslib/kstring.h $(HTSDIR)/htslib/kseq.h $(bcftools_h) cnv_h = HMM.h $(htslib_vcf_h) $(htslib_synced_bcf_reader_h) main.o: main.c $(htslib_hts_h) version.h $(bcftools_h) vcfannotate.o: vcfannotate.c $(htslib_vcf_h) $(htslib_synced_bcf_reader_h) $(HTSDIR)/htslib/kseq.h $(bcftools_h) vcmp.h $(filter_h) vcfplugin.o: vcfplugin.c $(htslib_vcf_h) $(htslib_synced_bcf_reader_h) $(HTSDIR)/htslib/kseq.h $(bcftools_h) vcmp.h $(filter_h) vcfcall.o: vcfcall.c $(htslib_vcf_h) $(HTSDIR)/htslib/kfunc.h $(htslib_synced_bcf_reader_h) $(bcftools_h) $(call_h) $(prob1_h) vcfconcat.o: vcfconcat.c $(htslib_vcf_h) $(htslib_synced_bcf_reader_h) $(HTSDIR)/htslib/kseq.h $(bcftools_h) vcfconvert.o: vcfconvert.c $(htslib_vcf_h) $(htslib_bgzf_h) $(htslib_synced_bcf_reader_h) $(htslib_vcfutils_h) $(bcftools_h) $(filter_h) $(convert_h) $(tsv2vcf_h) vcffilter.o: vcffilter.c $(htslib_vcf_h) $(htslib_synced_bcf_reader_h) $(htslib_vcfutils_h) $(bcftools_h) $(filter_h) rbuf.h vcfgtcheck.o: vcfgtcheck.c $(htslib_vcf_h) $(htslib_synced_bcf_reader_h) $(htslib_vcfutils_h) $(bcftools_h) vcfindex.o: vcfindex.c $(htslib_vcf_h) $(htslib_tbx_h) vcfisec.o: vcfisec.c $(htslib_vcf_h) $(htslib_synced_bcf_reader_h) $(htslib_vcfutils_h) $(bcftools_h) $(filter_h) vcfmerge.o: vcfmerge.c $(htslib_vcf_h) $(htslib_synced_bcf_reader_h) $(htslib_vcfutils_h) $(bcftools_h) vcmp.h $(HTSDIR)/htslib/khash.h vcfnorm.o: vcfnorm.c $(htslib_vcf_h) $(htslib_synced_bcf_reader_h) $(htslib_faidx_h) $(bcftools_h) rbuf.h vcfquery.o: vcfquery.c $(htslib_vcf_h) $(htslib_synced_bcf_reader_h) $(htslib_vcfutils_h) $(bcftools_h) $(filter_h) $(convert_h) vcfroh.o: vcfroh.c $(roh_h) vcfcnv.o: vcfcnv.c $(cnv_h) vcfsom.o: vcfsom.c $(htslib_vcf_h) $(htslib_synced_bcf_reader_h) $(htslib_vcfutils_h) $(bcftools_h) vcfstats.o: vcfstats.c $(htslib_vcf_h) $(htslib_synced_bcf_reader_h) $(htslib_vcfutils_h) $(htslib_faidx_h) $(bcftools_h) vcfview.o: vcfview.c $(htslib_vcf_h) $(htslib_synced_bcf_reader_h) $(htslib_vcfutils_h) $(bcftools_h) $(filter_h) reheader.o: reheader.c $(htslib_vcf_h) $(htslib_bgzf_h) $(HTSDIR)/htslib/kseq.h $(bcftools_h) tabix.o: tabix.c $(htslib_bgzf_h) $(htslib_tbx_h) ccall.o: ccall.c $(HTSDIR)/htslib/kfunc.h $(call_h) kmin.h $(prob1_h) convert.o: convert.c $(htslib_vcf_h) $(htslib_synced_bcf_reader_h) $(htslib_vcfutils_h) $(bcftools_h) $(convert_h) tsv2vcf.o: tsv2vcf.c $(tsv2vcf_h) em.o: em.c $(htslib_vcf_h) kmin.h $(call_h) filter.o: filter.c $(HTSDIR)/htslib/khash_str2int.h $(filter_h) $(bcftools_h) $(htslib_hts_defs_h) $(htslib_vcfutils_h) gvcf.o: gvcf.c $(call_h) kmin.o: kmin.c kmin.h mcall.o: mcall.c $(HTSDIR)/htslib/kfunc.h $(call_h) prob1.o: prob1.c $(prob1_h) vcmp.o: vcmp.c $(htslib_hts_h) vcmp.h polysomy.o: polysomy.c $(htslib_hts_h) consensus.o: consensus.c $(htslib_hts_h) $(HTSDIR)/htslib/kseq.h rbuf.h $(bcftools_h) $(HTSDIR)/htslib/regidx.h version.o: version.h version.c test/test-rbuf.o: test/test-rbuf.c rbuf.h test/test-rbuf: test/test-rbuf.o $(CC) $(CFLAGS) -o $@ -lm -ldl $< bcftools: $(HTSLIB) $(OBJS) $(CC) $(CFLAGS) -o $@ $(OBJS) $(HTSLIB) -lpthread -lz -lm -ldl $(LDLIBS) doc/bcftools.1: doc/bcftools.txt cd doc && a2x -adate="$(DOC_DATE)" -aversion=$(DOC_VERSION) --doctype manpage --format manpage bcftools.txt doc/bcftools.html: doc/bcftools.txt cd doc && a2x -adate="$(DOC_DATE)" -aversion=$(DOC_VERSION) --doctype manpage --format xhtml bcftools.txt docs: doc/bcftools.1 doc/bcftools.html install: $(PROG) doc/bcftools.1 $(INSTALL_DIR) $(DESTDIR)$(bindir) $(DESTDIR)$(man1dir) $(INSTALL_PROGRAM) $(PROG) plot-vcfstats vcfutils.pl $(DESTDIR)$(bindir) $(INSTALL_DATA) doc/bcftools.1 $(DESTDIR)$(man1dir) clean: testclean clean-plugins -rm -f gmon.out *.o *~ $(PROG) version.h plugins/*.so plugins/*.P -rm -rf *.dSYM plugins/*.dSYM test/*.dSYM clean-plugins: -rm -f plugins/*.so plugins/*.P plugins/*.dSYM testclean: -rm -f test/*.o test/*~ $(TEST_PROG) distclean: clean -rm -f TAGS clean-all: clean clean-htslib tags: ctags -f TAGS *.[ch] plugins/*.[ch] bcftools-1.2/README000066400000000000000000000003661246371514100140600ustar00rootroot00000000000000BCFtools implements utilities for variant calling (in conjunction with SAMtools) and manipulating VCF and BCF files. The program is intended to replace the Perl-based tools from vcftools. See INSTALL for building and installation instructions. bcftools-1.2/bcftools.h000066400000000000000000000042101246371514100151540ustar00rootroot00000000000000/* bcftools.h -- utility function declarations. Copyright (C) 2013 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #ifndef BCFTOOLS_H #define BCFTOOLS_H #include #include #define FT_GZ 1 #define FT_VCF 2 #define FT_VCF_GZ (FT_GZ|FT_VCF) #define FT_BCF (1<<2) #define FT_BCF_GZ (FT_GZ|FT_BCF) #define FT_STDIN (1<<3) char *bcftools_version(void); void error(const char *format, ...); void bcf_hdr_append_version(bcf_hdr_t *hdr, int argc, char **argv, const char *cmd); const char *hts_bcf_wmode(int file_type); void *smalloc(size_t size); // safe malloc static inline char gt2iupac(char a, char b) { static const char iupac[4][4] = { {'A','M','R','W'},{'M','C','S','Y'},{'R','S','G','K'},{'W','Y','K','T'} }; if ( a>='a' ) a -= 'a' - 'A'; if ( b>='a' ) b -= 'a' - 'A'; if ( a=='A' ) a = 0; else if ( a=='C' ) a = 1; else if ( a=='G' ) a = 2; else if ( a=='T' ) a = 3; else return 'N'; if ( b=='A' ) b = 0; else if ( b=='C' ) b = 1; else if ( b=='G' ) b = 2; else if ( b=='T' ) b = 3; else return 'N'; return iupac[(int)a][(int)b]; } #endif bcftools-1.2/call.h000066400000000000000000000115351246371514100142640ustar00rootroot00000000000000/* call.h -- variant calling declarations. Copyright (C) 2013-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #ifndef __CALL_H__ #define __CALL_H__ #include #include #include "vcmp.h" #define CALL_KEEPALT 1 #define CALL_VARONLY (1<<1) #define CALL_CONSTR_TRIO (1<<2) #define CALL_CONSTR_ALLELES (1<<3) #define CALL_CHR_X (1<<4) #define CALL_CHR_Y (1<<5) #define CALL_FMT_GQ (1<<6) #define CALL_FMT_GP (1<<7) #define FATHER 0 #define MOTHER 1 #define CHILD 2 typedef struct { char *name; int sample[3]; // father, mother, child int type; // see FTYPE_* definitions in mcall.c } family_t; typedef struct { int min_dp, mdp; // minimum per-sample depth of a gVCF block int32_t rid, start, end, *gt, *dp; char ref[2]; // reference base at start position bcf1_t *line; } gvcf_t; typedef struct _ccall_t ccall_t; typedef struct { // mcall only float *qsum; // QS(sum) values int nqsum, npdg; int *als_map, nals_map; // mapping from full set of alleles to trimmed set of alleles (old -> new) int *pl_map, npl_map; // same as above for PLs, but reverse (new -> old) char **als; // array to hold the trimmed set of alleles to appear on output int nals; // size of the als array family_t *fams; // list of families and samples for trio calling int nfams, mfams; int ntrio[5][5]; // possible trio genotype combinations and their counts; first idx: uint16_t *trio[5][5]; // family type, second index: allele count (2-4, first two are unused) double *GLs; float *GPs; // FORMAT/GP: posterior probabilities int32_t *GQs; // FORMAT/GQ: genotype qualities int32_t *itmp; // temporary int array, used for new PLs with CALL_CONSTR_ALLELES int n_itmp, nGPs; vcmp_t *vcmp; double trio_Pm_SNPs, trio_Pm_del, trio_Pm_ins; // P(mendelian) for trio calling, see mcall_call_trio_genotypes() int32_t *ugts, *cgts; // unconstraind and constrained GTs uint32_t output_tags; // ccall only double indel_frac, min_perm_p, min_lrt; double prior_type, pref; double ref_lk, lk_sum; int ngrp1_samples, n_perm; int nhets, ndiploid; char *prior_file; ccall_t *cdat; // shared bcf_srs_t *srs; // BCF synced readers holding target alleles for CALL_CONSTR_ALLELES bcf1_t *rec; bcf_hdr_t *hdr; uint32_t flag; // One or more of the CALL_* flags defined above uint8_t *ploidy, all_diploid; double pl2p[256]; // PL to 10^(-PL/10) table int32_t *PLs; // VCF PL likelihoods (rw) int nPLs, mPLs; int32_t *gts, ac[4]; // GTs and AC (w) double *pdg; // PLs converted to P(D|G) float *anno16; int n16; // see anno[16] in bam2bcf.h double theta; // prior } call_t; void error(const char *format, ...); /* * *call() - return negative value on error or the number of non-reference * alleles on success. */ int mcall(call_t *call, bcf1_t *rec); // multiallic and rare-variant calling model int ccall(call_t *call, bcf1_t *rec); // the default consensus calling model int qcall(call_t *call, bcf1_t *rec); // QCall output void mcall_init(call_t *call); void ccall_init(call_t *call); void qcall_init(call_t *call); void mcall_destroy(call_t *call); void ccall_destroy(call_t *call); void qcall_destroy(call_t *call); void call_init_pl2p(call_t *call); uint32_t *call_trio_prep(int is_x, int is_son); /** gVCF */ void gvcf_write(htsFile *fh, gvcf_t *gvcf, bcf_hdr_t *hdr, bcf1_t *rec, int is_ref); void init_allele_trimming_maps(call_t *call, int als, int nals); void mcall_trim_numberR(call_t *call, bcf1_t *rec, int nals, int nout_als, int out_als); #endif bcftools-1.2/ccall.c000066400000000000000000000324351246371514100144240ustar00rootroot00000000000000/* ccall.c -- consensus variant calling. Copyright (C) 2013-2014 Genome Research Ltd. Portions copyright (C) 2010 Broad Institute. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include "call.h" #include "kmin.h" #include "prob1.h" // Most of the original -c calling was moved to bcftools as it was // and its data structures were wrapped into the ccal_t to make it // functional quickly. This is not the desired state. struct _ccall_t { bcf_p1aux_t *p1; }; void ccall_init(call_t *call) { call->cdat = (ccall_t*) calloc(1,sizeof(ccall_t)); call_init_pl2p(call); call->cdat->p1 = bcf_p1_init(bcf_hdr_nsamples(call->hdr), call->ploidy); call->gts = (int*) calloc(bcf_hdr_nsamples(call->hdr)*2,sizeof(int)); // assuming at most diploid everywhere call->nals_map = 5; call->als_map = (int*) malloc(sizeof(int)*call->nals_map); bcf_hdr_append(call->hdr,"##FORMAT="); if ( call->output_tags & CALL_FMT_GQ ) { bcf_hdr_append(call->hdr,"##FORMAT="); call->GQs = (int32_t*) malloc(sizeof(int32_t)*bcf_hdr_nsamples(call->hdr)); } if ( call->output_tags & CALL_FMT_GP ) error("Sorry, -f GP is not supported with -c\n"); bcf_hdr_append(call->hdr,"##INFO="); // Todo: groups not migrated to 'bcftools call' yet bcf_hdr_append(call->hdr,"##INFO="); bcf_hdr_append(call->hdr,"##INFO="); bcf_hdr_append(call->hdr,"##INFO=\n"); bcf_hdr_append(call->hdr,"##INFO=\n"); bcf_hdr_append(call->hdr,"##INFO=\n"); bcf_hdr_append(call->hdr,"##INFO=\n"); bcf_hdr_append(call->hdr,"##INFO=\n"); // bcf_hdr_append(call->hdr,); // bcf_hdr_append(call->hdr,); bcf_hdr_append(call->hdr,"##INFO="); return; } void ccall_destroy(call_t *call) { free(call->itmp); free(call->als_map); free(call->gts); free(call->anno16); free(call->PLs); free(call->GQs); free(call->pdg); bcf_p1_destroy(call->cdat->p1); free(call->cdat); return; } // Inits P(D|G): convert PLs from log space, only two alleles (three PLs) are used. // NB: The original samtools calling code uses pdgs in reverse order (AA comes // first, RR last), while the -m calling model uses the canonical order. static void set_pdg3(double *pl2p, int *PLs, double *pdg, int n_smpl, int n_gt) { int i; for (i=0; ip[0] = a->p[1] = a->p[2] = a->p[3] = 1.; for (i=0; i<4; i++) a->d[i] = anno[i]; a->depth = anno[0] + anno[1] + anno[2] + anno[3]; a->is_tested = (anno[0] + anno[1] > 0 && anno[2] + anno[3] > 0); if (a->depth == 0) return -1; a->mq = (int)(sqrt((anno[9] + anno[11]) / a->depth) + .499); kt_fisher_exact(anno[0], anno[1], anno[2], anno[3], &left, &right, &a->p[0]); for (i = 1; i < 4; ++i) a->p[i] = ttest(anno[0] + anno[1], anno[2] + anno[3], anno+4*i); return 0; } int test16(float *anno16, anno16_t *a) { a->p[0] = a->p[1] = a->p[2] = a->p[3] = 1.; a->d[0] = a->d[1] = a->d[2] = a->d[3] = 0.; a->mq = a->depth = a->is_tested = 0; return test16_core(anno16, a); } static int update_bcf1(call_t *call, bcf1_t *rec, const bcf_p1rst_t *pr, double em[10]) { int has_I16, is_var; float fq, r; anno16_t a; float tmpf[4], tmpi; bcf_get_info_float(call->hdr, rec, "I16", &call->anno16, &call->n16); has_I16 = test16(call->anno16, &a) >= 0? 1 : 0; // print EM if (em[0] >= 0) { tmpf[0] = 1 - em[0]; bcf_update_info_float(call->hdr, rec, "AF1", tmpf, 1); } if (em[4] >= 0 && em[4] <= 0.05) { tmpf[0] = em[3]; tmpf[1] = em[2]; tmpf[2] = em[1]; tmpf[3] = em[4]; bcf_update_info_float(call->hdr, rec, "G3", tmpf, 3); bcf_update_info_float(call->hdr, rec, "HWE", &tmpf[3], 1); } if (em[5] >= 0 && em[6] >= 0) { tmpf[0] = 1 - em[5]; tmpf[1] = 1 - em[6]; bcf_update_info_float(call->hdr, rec, "AF2", tmpf, 2); } if (em[7] >= 0) { tmpf[0] = em[7]; bcf_update_info_float(call->hdr, rec, "LRT", tmpf, 1); } if (em[8] >= 0) { tmpf[0] = em[8]; bcf_update_info_float(call->hdr, rec, "LRT2", tmpf, 1); } bcf_p1aux_t *p1 = call->cdat->p1; if (p1->cons_llr > 0) { tmpi = p1->cons_llr; bcf_update_info_int32(call->hdr, rec, "CLR", &tmpi, 1); // todo: trio calling with -c if (p1->cons_gt > 0) { char tmp[4]; tmp[0] = p1->cons_gt&0xff; tmp[1] = p1->cons_gt>>8&0xff; tmp[2] = p1->cons_gt>>16&0xff; tmp[3] = 0; bcf_update_info_string(call->hdr, rec, "UGT", tmp); tmp[0] = p1->cons_gt>>32&0xff; tmp[1] = p1->cons_gt>>40&0xff; tmp[2] = p1->cons_gt>>48&0xff; bcf_update_info_string(call->hdr, rec, "CGT", tmp); } } if (pr == 0) return 1; is_var = (pr->p_ref < call->pref); r = is_var? pr->p_ref : pr->p_var; bcf_update_info_int32(call->hdr, rec, "AC1", &pr->ac, 1); int32_t dp[4]; dp[0] = call->anno16[0]; dp[1] = call->anno16[1]; dp[2] = call->anno16[2]; dp[3] = call->anno16[3]; bcf_update_info_int32(call->hdr, rec, "DP4", dp, 4); bcf_update_info_int32(call->hdr, rec, "MQ", &a.mq, 1); fq = pr->p_ref_folded < 0.5? -4.343 * log(pr->p_ref_folded) : 4.343 * log(pr->p_var_folded); if (fq < -999) fq = -999; if (fq > 999) fq = 999; bcf_update_info_float(call->hdr, rec, "FQ", &fq, 1); assert( pr->cmp[0]<0 ); // todo // if (pr->cmp[0] >= 0.) { // two sample groups // int i, q[3]; // for (i = 1; i < 3; ++i) { // double x = pr->cmp[i] + pr->cmp[0]/2.; // q[i] = x == 0? 255 : (int)(-4.343 * log(x) + .499); // if (q[i] > 255) q[i] = 255; // } // if (pr->perm_rank >= 0) ksprintf(&s, "PR=%d;", pr->perm_rank); // // ksprintf(&s, "PCHI2=%.3g;PC2=%d,%d;", q[1], q[2], pr->p_chi2); // } if (has_I16 && a.is_tested) { int i; for (i=0; i<4; i++) tmpf[i] = a.p[i]; bcf_update_info_float(call->hdr, rec, "PV4", tmpf, 4); } bcf_update_info_int32(call->hdr, rec, "I16", NULL, 0); // remove I16 tag bcf_update_info_int32(call->hdr, rec, "QS", NULL, 0); // remove QS tag rec->qual = r < 1e-100? 999 : -4.343 * log(r); if (rec->qual > 999) rec->qual = 999; // Remove unused alleles int nals_ori = rec->n_allele, nals = !is_var && !(call->flag & CALL_KEEPALT) ? 1 : pr->rank0 < 2? 2 : pr->rank0+1; if ( call->flag & CALL_KEEPALT && nals>1 ) { if ( rec->d.allele[nals-1][0]=='X' ) nals--; // old version of unseen allele "X" else if ( rec->d.allele[nals-1][0]=='<' && rec->d.allele[nals-1][1]=='X' && rec->d.allele[nals-1][2]=='>' ) nals--; // old version of unseen allele, "" else if ( rec->d.allele[nals-1][0]=='<' && rec->d.allele[nals-1][1]=='*' && rec->d.allele[nals-1][2]=='>' ) nals--; // new version of unseen allele, "<*>" } if ( nalsn_allele ) { bcf_update_alleles(call->hdr, rec, (const char**)rec->d.allele, nals); // Update PLs int npls_src = call->nPLs / rec->n_sample, npls_dst = nals*(nals+1)/2; int *pls_src = call->PLs - npls_src, *pls_dst = call->PLs - npls_dst; int isample, i; for (isample = 0; isample < rec->n_sample; isample++) { pls_src += npls_src; pls_dst += npls_dst; if ( !call->ploidy || call->ploidy[isample]==2 ) { for (i=0; ihdr, rec, "PL", call->PLs, npls_dst*rec->n_sample); } // Call genotypes int i; for (i=0; in_sample; i++) { int x = ( is_var || call->output_tags & CALL_FMT_GQ ) ? bcf_p1_call_gt(p1, pr->f_exp, i) : 2; int gt = x&3; if ( !call->ploidy || call->ploidy[i]==2 ) { if ( gt==1 ) { call->gts[2*i] = bcf_gt_unphased(0); call->gts[2*i+1] = bcf_gt_unphased(1); } else if ( gt==0 ) { call->gts[2*i] = bcf_gt_unphased(1); call->gts[2*i+1] = bcf_gt_unphased(1); } else { call->gts[2*i] = bcf_gt_unphased(0); call->gts[2*i+1] = bcf_gt_unphased(0); } if ( call->output_tags & CALL_FMT_GQ ) call->GQs[i] = x>>2; } else { if ( gt==0 ) call->gts[2*i] = bcf_gt_unphased(1); else call->gts[2*i] = bcf_gt_unphased(0); call->gts[2*i+1] = bcf_int32_vector_end; if ( call->output_tags & CALL_FMT_GQ ) call->GQs[i] = bcf_int32_missing; } } bcf_update_genotypes(call->hdr, rec, call->gts, rec->n_sample*2); if ( call->output_tags & CALL_FMT_GQ ) bcf_update_format_int32(call->hdr, rec, "GQ", call->GQs, rec->n_sample); // trim Number=R tags int out_als = 0; for (i=0; ihdr); // Get the genotype likelihoods int nals = rec->n_allele; call->nPLs = bcf_get_format_int32(call->hdr, rec, "PL", &call->PLs, &call->mPLs); if ( call->nPLs!=nsmpl*nals*(nals+1)/2 && call->nPLs!=nsmpl*nals ) // diploid+haploid or haploid only error("Wrong number of PL fields? nals=%d npl=%d\n", nals,call->nPLs); // Convert PLs to probabilities, only first two alleles are considered int ngts = nals*(nals+1)/2; hts_expand(double, 3*nsmpl, call->npdg, call->pdg); set_pdg3(call->pl2p, call->PLs, call->pdg, nsmpl, ngts); double em[10] = {-1.,-1.,-1.,-1.,-1.,-1.,-1.,-1.,-1.,-1.}; int ret = bcf_em1(call, rec, call->ngrp1_samples, 0x1ff, em); bcf_p1rst_t pr; int do_contrast = (em[7] >= 0 && em[7] < call->min_lrt) ? 1 : 0; ret = bcf_p1_cal(call, rec, do_contrast, call->cdat->p1, &pr); if (pr.p_ref >= call->pref && (call->flag & CALL_VARONLY)) return 0; if (ret >= 0) ret = update_bcf1(call, rec, &pr, em); return ret; } bcftools-1.2/consensus.c000066400000000000000000000610631246371514100153650ustar00rootroot00000000000000/* The MIT License Copyright (c) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include #include "bcftools.h" #include "rbuf.h" typedef struct { int num; // number of ungapped blocks in this chain int *block_lengths; // length of the ungapped blocks in this chain int *ref_gaps; // length of the gaps on the reference sequence between blocks int *alt_gaps; // length of the gaps on the alternative sequence between blocks int ori_pos; int ref_last_block_ori; // start position on the reference sequence of the following ungapped block (0-based) int alt_last_block_ori; // start position on the alternative sequence of the following ungapped block (0-based) } chain_t; typedef struct { kstring_t fa_buf; // buffered reference sequence int fa_ori_pos; // start position of the fa_buffer (wrt original sequence) int fa_frz_pos; // protected position to avoid conflicting variants (last pos for SNPs/ins) int fa_mod_off; // position difference of fa_frz_pos in the ori and modified sequence (ins positive) int fa_end_pos; // region's end position in the original sequence int fa_length; // region's length in the original sequence (in case end_pos not provided in the FASTA header) int fa_case; // output upper case or lower case? int fa_src_pos; // last genomic coordinate read from the input fasta (0-based) rbuf_t vcf_rbuf; bcf1_t **vcf_buf; int nvcf_buf, rid; regidx_t *mask; int chain_id; // chain_id, to provide a unique ID to each chain in the chain output chain_t *chain; // chain structure to store the sequence of ungapped blocks between the ref and alt sequences // Note that the chain is re-initialised for each chromosome/seq_region bcf_srs_t *files; bcf_hdr_t *hdr; FILE *fp_out; FILE *fp_chain; char **argv; int argc, output_iupac, haplotype, isample; char *fname, *ref_fname, *sample, *output_fname, *mask_fname, *chain_fname; } args_t; static chain_t* init_chain(chain_t *chain, int ref_ori_pos) { // fprintf(stderr, "init_chain(*chain, ref_ori_pos=%d)\n", ref_ori_pos); chain = (chain_t*) calloc(1,sizeof(chain_t)); chain->num = 0; chain->block_lengths = NULL; chain->ref_gaps = NULL; chain->alt_gaps = NULL; chain->ori_pos = ref_ori_pos; chain->ref_last_block_ori = ref_ori_pos; chain->alt_last_block_ori = ref_ori_pos; return chain; } static void destroy_chain(args_t *args) { chain_t *chain = args->chain; free(chain->ref_gaps); free(chain->alt_gaps); free(chain->block_lengths); free(chain); chain = NULL; } static void print_chain(args_t *args) { /* Example chain format (see: https://genome.ucsc.edu/goldenPath/help/chain.html): chain 1 500 + 480 500 1 501 + 480 501 1 12 3 1 1 0 3 484 chain line is: - chain - score (sum of the length of ungapped block in this case) - ref_seqname (from the fasta header, parsed by htslib) - ref_seqlength (from the fasta header) - ref_strand (+ or -; always + for bcf-consensus) - ref_start (as defined in the fasta header) - ref_end (as defined in the fasta header) - alt_seqname (same as ref_seqname as bcf-consensus only considers SNPs and indels) - alt_seqlength (adjusted to match the length of the alt sequence) - alt_strand (+ or -; always + for bcf-consensus) - alt_start (same as ref_start, as no edits are recorded/applied before that position) - alt_end (adjusted to match the length of the alt sequence) - chain_num (just an auto-increment id) the other (sorted) lines are: - length of the ungapped alignment block - gap on the ref sequence between this and the next block (all but the last line) - gap on the alt sequence between this and the next block (all but the last line) */ chain_t *chain = args->chain; int n = chain->num; int ref_end_pos = args->fa_length + chain->ori_pos; int last_block_size = ref_end_pos - chain->ref_last_block_ori; int alt_end_pos = chain->alt_last_block_ori + last_block_size; int score = 0; for (n=0; nnum; n++) { score += chain->block_lengths[n]; } score += last_block_size; fprintf(args->fp_chain, "chain %d %s %d + %d %d %s %d + %d %d %d\n", score, bcf_hdr_id2name(args->hdr,args->rid), ref_end_pos, chain->ori_pos, ref_end_pos, bcf_hdr_id2name(args->hdr,args->rid), alt_end_pos, chain->ori_pos, alt_end_pos, ++args->chain_id); for (n=0; nnum; n++) { fprintf(args->fp_chain, "%d %d %d\n", chain->block_lengths[n], chain->ref_gaps[n], chain->alt_gaps[n]); } fprintf(args->fp_chain, "%d\n\n", last_block_size); } static void push_chain_gap(chain_t *chain, int ref_start, int ref_len, int alt_start, int alt_len) { // fprintf(stderr, "push_chain_gap(*chain, ref_start=%d, ref_len=%d, alt_start=%d, alt_len=%d)\n", ref_start, ref_len, alt_start, alt_len); int num = chain->num; if (ref_start <= chain->ref_last_block_ori) { // In case this variant is back-to-back with the previous one chain->ref_last_block_ori = ref_start + ref_len; chain->alt_last_block_ori = alt_start + alt_len; chain->ref_gaps[num-1] += ref_len; chain->alt_gaps[num-1] += alt_len; } else { // Extend the ungapped blocks, store the gap length chain->block_lengths = (int*) realloc(chain->block_lengths, (num + 1) * sizeof(int)); chain->ref_gaps = (int*) realloc(chain->ref_gaps, (num + 1) * sizeof(int)); chain->alt_gaps = (int*) realloc(chain->alt_gaps, (num + 1) * sizeof(int)); chain->block_lengths[num] = ref_start - chain->ref_last_block_ori; chain->ref_gaps[num] = ref_len; chain->alt_gaps[num] = alt_len; // Update the start positions of the next block chain->ref_last_block_ori = ref_start + ref_len; chain->alt_last_block_ori = alt_start + alt_len; // Increment the number of ungapped blocks chain->num++; } } static void init_data(args_t *args) { args->files = bcf_sr_init(); args->files->require_index = 1; if ( !bcf_sr_add_reader(args->files,args->fname) ) error("Failed to open %s: %s\n", args->fname, bcf_sr_strerror(args->files->errnum)); args->hdr = args->files->readers[0].header; args->isample = -1; if ( args->sample ) { args->isample = bcf_hdr_id2int(args->hdr,BCF_DT_SAMPLE,args->sample); if ( args->isample<0 ) error("No such sample: %s\n", args->sample); } if ( args->haplotype && args->isample<0 ) { if ( bcf_hdr_nsamples(args->hdr) > 1 ) error("The --sample option is expected with --haplotype\n"); args->isample = 0; } if ( args->mask_fname ) { args->mask = regidx_init(args->mask_fname,NULL,NULL,0,NULL); if ( !args->mask ) error("Failed to initialize mask regions\n"); } // In case we want to store the chains if ( args->chain_fname ) { args->fp_chain = fopen(args->chain_fname,"w"); args->chain_id = 0; } rbuf_init(&args->vcf_rbuf, 100); args->vcf_buf = (bcf1_t**) calloc(args->vcf_rbuf.m, sizeof(bcf1_t*)); args->fp_out = args->output_fname ? fopen(args->output_fname,"w") : stdout; } static void destroy_data(args_t *args) { bcf_sr_destroy(args->files); int i; for (i=0; ivcf_rbuf.m; i++) if ( args->vcf_buf[i] ) bcf_destroy1(args->vcf_buf[i]); free(args->vcf_buf); free(args->fa_buf.s); if ( args->mask ) regidx_destroy(args->mask); if ( args->chain_fname ) if ( fclose(args->fp_chain) ) error("Close failed: %s\n", args->chain_fname); if ( fclose(args->fp_out) ) error("Close failed: %s\n", args->output_fname); } static void init_region(args_t *args, char *line) { char *ss, *se = line; while ( *se && !isspace(*se) && *se!=':' ) se++; int from = 0, to = 0; char tmp, *tmp_ptr = NULL; if ( *se ) { tmp = *se; *se = 0; tmp_ptr = se; ss = ++se; from = strtol(ss,&se,10); if ( ss==se || !*se || *se!='-' ) from = 0; else { from--; ss = ++se; to = strtol(ss,&se,10); if ( ss==se || (*se && !isspace(*se)) ) { from = 0; to = 0; } else to--; } } args->rid = bcf_hdr_name2id(args->hdr,line); if ( args->rid<0 ) fprintf(stderr,"Warning: Sequence \"%s\" not in %s\n", line,args->fname); args->fa_buf.l = 0; args->fa_length = 0; args->fa_end_pos = to; args->fa_ori_pos = from; args->fa_src_pos = from; args->fa_mod_off = 0; args->fa_frz_pos = -1; args->fa_case = -1; args->vcf_rbuf.n = 0; bcf_sr_seek(args->files,line,args->fa_ori_pos); if ( tmp_ptr ) *tmp_ptr = tmp; fprintf(args->fp_out,">%s\n",line); if (args->chain_fname ) { args->chain = init_chain(args->chain, args->fa_ori_pos); } else { args->chain = NULL; } } static bcf1_t **next_vcf_line(args_t *args) { if ( args->vcf_rbuf.n ) { int i = rbuf_shift(&args->vcf_rbuf); return &args->vcf_buf[i]; } else if ( bcf_sr_next_line(args->files) ) return &args->files->readers[0].buffer[0]; return NULL; } static void unread_vcf_line(args_t *args, bcf1_t **rec_ptr) { bcf1_t *rec = *rec_ptr; if ( args->vcf_rbuf.n >= args->vcf_rbuf.m ) error("FIXME: too many overlapping records near %s:%d\n", bcf_seqname(args->hdr,rec),rec->pos+1); // Insert the new record in the buffer. The line would be overwritten in // the next bcf_sr_next_line call, therefore we need to swap it with an // unused one int i = rbuf_append(&args->vcf_rbuf); if ( !args->vcf_buf[i] ) args->vcf_buf[i] = bcf_init1(); bcf1_t *tmp = rec; *rec_ptr = args->vcf_buf[i]; args->vcf_buf[i] = tmp; } static void flush_fa_buffer(args_t *args, int len) { if ( !args->fa_buf.l ) return; int nwr = 0; while ( nwr + 60 <= args->fa_buf.l ) { if ( fwrite(args->fa_buf.s+nwr,1,60,args->fp_out) != 60 ) error("Could not write: %s\n", args->output_fname); if ( fwrite("\n",1,1,args->fp_out) != 1 ) error("Could not write: %s\n", args->output_fname); nwr += 60; } if ( nwr ) args->fa_ori_pos += nwr; if ( len ) { // not finished on this chr yet and the buffer cannot be emptied completely if ( nwr && nwr < args->fa_buf.l ) memmove(args->fa_buf.s,args->fa_buf.s+nwr,args->fa_buf.l-nwr); args->fa_buf.l -= nwr; return; } // empty the whole buffer if ( nwr == args->fa_buf.l ) { args->fa_buf.l = 0; return; } if ( fwrite(args->fa_buf.s+nwr,1,args->fa_buf.l - nwr,args->fp_out) != args->fa_buf.l - nwr ) error("Could not write: %s\n", args->output_fname); if ( fwrite("\n",1,1,args->fp_out) != 1 ) error("Could not write: %s\n", args->output_fname); args->fa_ori_pos += args->fa_buf.l - nwr - args->fa_mod_off; args->fa_mod_off = 0; args->fa_buf.l = 0; } static void apply_variant(args_t *args, bcf1_t *rec) { if ( rec->n_allele==1 ) return; if ( rec->pos <= args->fa_frz_pos ) { fprintf(stderr,"The site %s:%d overlaps with another variant, skipping...\n", bcf_seqname(args->hdr,rec),rec->pos+1); return; } if ( args->mask ) { char *chr = (char*)bcf_hdr_id2name(args->hdr,args->rid); int start = rec->pos; int end = rec->pos + rec->rlen - 1; if ( regidx_overlap(args->mask, chr,start,end,NULL) ) return; } int i, ialt = 1; if ( args->isample >= 0 ) { bcf_fmt_t *fmt = bcf_get_fmt(args->hdr, rec, "GT"); if ( !fmt ) return; if ( args->haplotype ) { if ( args->haplotype > fmt->n ) error("Can't apply %d-th haplotype at %s:%d\n", args->haplotype,bcf_seqname(args->hdr,rec),rec->pos+1); uint8_t *ignore, *ptr = fmt->p + fmt->size*args->isample + args->haplotype - 1; ialt = bcf_dec_int1(ptr, fmt->type, &ignore); if ( bcf_gt_is_missing(ialt) || ialt==bcf_int32_vector_end ) return; ialt = bcf_gt_allele(ialt); } else if ( args->output_iupac ) { uint8_t *ignore, *ptr = fmt->p + fmt->size*args->isample; ialt = bcf_dec_int1(ptr, fmt->type, &ignore); if ( bcf_gt_is_missing(ialt) || ialt==bcf_int32_vector_end ) return; ialt = bcf_gt_allele(ialt); int jalt; if ( fmt->n>1 ) { ptr = fmt->p + fmt->size*args->isample + 1; jalt = bcf_dec_int1(ptr, fmt->type, &ignore); if ( bcf_gt_is_missing(jalt) || jalt==bcf_int32_vector_end ) jalt = ialt; else jalt = bcf_gt_allele(jalt); } else jalt = ialt; if ( rec->n_allele <= ialt || rec->n_allele <= jalt ) error("Broken VCF, too few alts at %s:%d\n", bcf_seqname(args->hdr,rec),rec->pos+1); if ( ialt!=jalt && !rec->d.allele[ialt][1] && !rec->d.allele[jalt][1] ) // is this a het snp? { char ial = rec->d.allele[ialt][0]; char jal = rec->d.allele[jalt][0]; rec->d.allele[ialt][0] = gt2iupac(ial,jal); } } else { for (i=0; in; i++) { uint8_t *ignore, *ptr = fmt->p + fmt->size*args->isample + i; ialt = bcf_dec_int1(ptr, fmt->type, &ignore); if ( bcf_gt_is_missing(ialt) || ialt==bcf_int32_vector_end ) return; ialt = bcf_gt_allele(ialt); if ( ialt ) break; } } if ( !ialt ) return; // ref allele if ( rec->n_allele <= ialt ) error("Broken VCF, too few alts at %s:%d\n", bcf_seqname(args->hdr,rec),rec->pos+1); } else if ( args->output_iupac && !rec->d.allele[0][1] && !rec->d.allele[1][1] ) { char ial = rec->d.allele[0][0]; char jal = rec->d.allele[1][0]; rec->d.allele[1][0] = gt2iupac(ial,jal); } int idx = rec->pos - args->fa_ori_pos + args->fa_mod_off; if ( idx<0 || idx>=args->fa_buf.l ) error("FIXME: %s:%d .. idx=%d, ori_pos=%d, len=%d, off=%d\n",bcf_seqname(args->hdr,rec),rec->pos+1,idx,args->fa_ori_pos,args->fa_buf.l,args->fa_mod_off); // sanity check the reference base int len_diff = 0, alen = 0; if ( rec->d.allele[ialt][0]=='<' ) { if ( strcasecmp(rec->d.allele[ialt], "") ) error("Symbolic alleles other than are currently not supported: %s at %s:%d\n",rec->d.allele[ialt],bcf_seqname(args->hdr,rec),rec->pos+1); assert( rec->d.allele[0][1]==0 ); // todo: for now expecting strlen(REF) = 1 len_diff = 1-rec->rlen; rec->d.allele[ialt] = rec->d.allele[0]; // according to VCF spec, REF must precede the event alen = strlen(rec->d.allele[ialt]); } else if ( strncasecmp(rec->d.allele[0],args->fa_buf.s+idx,rec->rlen) ) { // fprintf(stderr,"%d .. [%s], idx=%d ori=%d off=%d\n",args->fa_ori_pos,args->fa_buf.s,idx,args->fa_ori_pos,args->fa_mod_off); char tmp = 0; if ( args->fa_buf.l - idx > rec->rlen ) { tmp = args->fa_buf.s[idx+rec->rlen]; args->fa_buf.s[idx+rec->rlen] = 0; } error( "The fasta sequence does not match the REF allele at %s:%d:\n" " .vcf: [%s]\n" " .vcf: [%s] <- (ALT)\n" " .fa: [%s]%c%s\n", bcf_seqname(args->hdr,rec),rec->pos+1, rec->d.allele[0], rec->d.allele[ialt], args->fa_buf.s+idx, tmp?tmp:' ',tmp?args->fa_buf.s+idx+rec->rlen+1:"" ); } else { alen = strlen(rec->d.allele[ialt]); len_diff = alen - rec->rlen; } if ( args->fa_case ) for (i=0; id.allele[ialt][i] = toupper(rec->d.allele[ialt][i]); else for (i=0; id.allele[ialt][i] = tolower(rec->d.allele[ialt][i]); if ( len_diff <= 0 ) { // deletion or same size event for (i=0; ifa_buf.s[idx+i] = rec->d.allele[ialt][i]; if ( len_diff ) memmove(args->fa_buf.s+idx+alen,args->fa_buf.s+idx+rec->rlen,args->fa_buf.l-idx-rec->rlen); } else { // insertion ks_resize(&args->fa_buf, args->fa_buf.l + len_diff); memmove(args->fa_buf.s + idx + rec->rlen + len_diff, args->fa_buf.s + idx + rec->rlen, args->fa_buf.l - idx - rec->rlen); for (i=0; ifa_buf.s[idx+i] = rec->d.allele[ialt][i]; } if (args->chain && len_diff != 0) { // If first nucleotide of both REF and ALT are the same... (indels typically include the nucleotide before the variant) if ( strncasecmp(rec->d.allele[0],rec->d.allele[ialt],1) == 0) { // ...extend the block by 1 bp: start is 1 bp further and alleles are 1 bp shorter push_chain_gap(args->chain, rec->pos + 1, rec->rlen - 1, rec->pos + 1 + args->fa_mod_off, alen - 1); } else { // otherwise, just the coordinates of the variant as given push_chain_gap(args->chain, rec->pos, rec->rlen, rec->pos + args->fa_mod_off, alen); } } args->fa_buf.l += len_diff; args->fa_mod_off += len_diff; args->fa_frz_pos = rec->pos + rec->rlen - 1; } static void mask_region(args_t *args, char *seq, int len) { char *chr = (char*)bcf_hdr_id2name(args->hdr,args->rid); int start = args->fa_src_pos - len; int end = args->fa_src_pos; regitr_t itr; if ( !regidx_overlap(args->mask, chr,start,end, &itr) ) return; int idx_start, idx_end, i; while ( REGITR_OVERLAP(itr,start,end) ) { idx_start = REGITR_START(itr) - start; idx_end = REGITR_END(itr) - start; if ( idx_start < 0 ) idx_start = 0; if ( idx_end >= len ) idx_end = len - 1; for (i=idx_start; i<=idx_end; i++) seq[i] = 'N'; itr.i++; } } static void consensus(args_t *args) { htsFile *fasta = hts_open(args->ref_fname, "rb"); if ( !fasta ) error("Error reading %s\n", args->ref_fname); kstring_t str = {0,0,0}; while ( hts_getline(fasta, KS_SEP_LINE, &str) > 0 ) { if ( str.s[0]=='>' ) { // new sequence encountered, apply all chached variants while ( args->vcf_rbuf.n ) { if (args->chain) { print_chain(args); destroy_chain(args); } bcf1_t *rec = args->vcf_buf[args->vcf_rbuf.f]; if ( rec->rid!=args->rid || ( args->fa_end_pos && rec->pos > args->fa_end_pos ) ) break; int i = rbuf_shift(&args->vcf_rbuf); apply_variant(args, args->vcf_buf[i]); } flush_fa_buffer(args, 0); init_region(args, str.s+1); continue; } args->fa_length += str.l; args->fa_src_pos += str.l; // determine if uppercase or lowercase is used in this fasta file if ( args->fa_case==-1 ) args->fa_case = toupper(str.s[0])==str.s[0] ? 1 : 0; if ( args->mask && args->rid>=0) mask_region(args, str.s, str.l); kputs(str.s, &args->fa_buf); bcf1_t **rec_ptr = NULL; while ( args->rid>=0 && (rec_ptr = next_vcf_line(args)) ) { bcf1_t *rec = *rec_ptr; // still the same chr and the same region? if not, fasta buf can be flushed if ( rec->rid!=args->rid || ( args->fa_end_pos && rec->pos > args->fa_end_pos ) ) { // save the vcf record until next time and flush unread_vcf_line(args, rec_ptr); rec_ptr = NULL; break; } // is the vcf record well beyond cached fasta buffer? if yes, the buf can be flushed if ( args->fa_ori_pos + args->fa_buf.l - args->fa_mod_off <= rec->pos ) { unread_vcf_line(args, rec_ptr); rec_ptr = NULL; break; } // is the cached fasta buffer full enough? if not, read more fasta, no flushing if ( args->fa_ori_pos + args->fa_buf.l - args->fa_mod_off <= rec->pos + rec->rlen ) { unread_vcf_line(args, rec_ptr); break; } apply_variant(args, rec); } if ( !rec_ptr ) flush_fa_buffer(args, 60); } if (args->chain) { print_chain(args); destroy_chain(args); } flush_fa_buffer(args, 0); hts_close(fasta); free(str.s); } static void usage(args_t *args) { fprintf(stderr, "\n"); fprintf(stderr, "About: Create consensus sequence by applying VCF variants to a reference\n"); fprintf(stderr, " fasta file.\n"); fprintf(stderr, "Usage: bcftools consensus [OPTIONS] \n"); fprintf(stderr, "Options:\n"); fprintf(stderr, " -f, --fasta-ref reference sequence in fasta format\n"); fprintf(stderr, " -H, --haplotype <1|2> apply variants for the given haplotype\n"); fprintf(stderr, " -i, --iupac-codes output variants in the form of IUPAC ambiguity codes\n"); fprintf(stderr, " -m, --mask replace regions with N\n"); fprintf(stderr, " -o, --output write output to a file [standard output]\n"); fprintf(stderr, " -c, --chain write a chain file for liftover\n"); fprintf(stderr, " -s, --sample apply variants of the given sample\n"); fprintf(stderr, "Examples:\n"); fprintf(stderr, " # Get the consensus for one region. The fasta header lines are then expected\n"); fprintf(stderr, " # in the form \">chr:from-to\".\n"); fprintf(stderr, " samtools faidx ref.fa 8:11870-11890 | bcftools consensus in.vcf.gz > out.fa\n"); fprintf(stderr, "\n"); exit(1); } int main_consensus(int argc, char *argv[]) { args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->argc = argc; args->argv = argv; static struct option loptions[] = { {"sample",1,0,'s'}, {"iupac-codes",0,0,'i'}, {"haplotype",1,0,'H'}, {"output",1,0,'o'}, {"fasta-ref",1,0,'f'}, {"mask",1,0,'m'}, {"chain",1,0,'c'}, {0,0,0,0} }; char c; while ((c = getopt_long(argc, argv, "h?s:1iH:f:o:m:c:",loptions,NULL)) >= 0) { switch (c) { case 's': args->sample = optarg; break; case 'o': args->output_fname = optarg; break; case 'i': args->output_iupac = 1; break; case 'f': args->ref_fname = optarg; break; case 'm': args->mask_fname = optarg; break; case 'c': args->chain_fname = optarg; break; case 'H': args->haplotype = optarg[0] - '0'; if ( args->haplotype <=0 ) error("Expected positive integer with --haplotype\n"); break; default: usage(args); break; } } if ( optind>=argc ) usage(args); args->fname = argv[optind]; if ( !args->ref_fname && !isatty(fileno((FILE *)stdin)) ) args->ref_fname = "-"; if ( !args->ref_fname ) usage(args); init_data(args); consensus(args); destroy_data(args); free(args); return 0; } bcftools-1.2/convert.c000066400000000000000000001050641246371514100150250ustar00rootroot00000000000000/* convert.c -- functions for converting between VCF/BCF and related formats. Copyright (C) 2013-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include #include #include #include "bcftools.h" #include "convert.h" #define T_CHROM 1 #define T_POS 2 #define T_ID 3 #define T_REF 4 #define T_ALT 5 #define T_QUAL 6 #define T_FILTER 7 #define T_INFO 8 #define T_FORMAT 9 #define T_SAMPLE 10 #define T_SEP 11 #define T_IS_TS 12 #define T_TYPE 13 #define T_MASK 14 #define T_GT 15 #define T_TGT 16 #define T_LINE 17 #define T_CHROM_POS_ID 18 // not publicly advertised #define T_GT_TO_PROB3 19 // not publicly advertised #define T_PL_TO_PROB3 20 // not publicly advertised #define T_GP_TO_PROB3 21 // not publicly advertised #define T_FIRST_ALT 22 // not publicly advertised #define T_IUPAC_GT 23 #define T_GT_TO_HAP 24 // not publicly advertised #define T_GT_TO_HAP2 25 // not publicly advertised typedef struct _fmt_t { int type, id, is_gt_field, ready, subscript; char *key; bcf_fmt_t *fmt; void (*handler)(convert_t *, bcf1_t *, struct _fmt_t *, int, kstring_t *); } fmt_t; struct _convert_t { fmt_t *fmt; int nfmt, mfmt; int nsamples, *samples; bcf_hdr_t *header; int max_unpack; char *format_str; bcf_srs_t *readers; // required only for %MASK int nreaders; void *dat; int ndat; }; static void process_chrom(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { kputs(convert->header->id[BCF_DT_CTG][line->rid].key, str); } static void process_pos(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { kputw(line->pos+1, str); } static void process_id(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { kputs(line->d.id, str); } static void process_ref(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { kputs(line->d.allele[0], str); } static void process_alt(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { int i; if ( line->n_allele==1 ) { kputc('.', str); return; } if ( fmt->subscript>=0 ) { if ( line->n_allele > fmt->subscript+1 ) kputs(line->d.allele[fmt->subscript+1], str); else kputc('.', str); return; } for (i=1; in_allele; i++) { if ( i>1 ) kputc(',', str); kputs(line->d.allele[i], str); } } static void process_first_alt(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { if ( line->n_allele==1 ) kputc('.', str); else kputs(line->d.allele[1], str); } static void process_qual(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { if ( bcf_float_is_missing(line->qual) ) kputc('.', str); else ksprintf(str, "%g", line->qual); } static void process_filter(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { int i; if ( line->d.n_flt ) { for (i=0; id.n_flt; i++) { if (i) kputc(';', str); kputs(convert->header->id[BCF_DT_ID][line->d.flt[i]].key, str); } } else kputc('.', str); } static inline int bcf_array_ivalue(void *bcf_array, int type, int idx) { if ( type==BCF_BT_INT8 ) return ((int8_t*)bcf_array)[idx]; if ( type==BCF_BT_INT16 ) return ((int16_t*)bcf_array)[idx]; return ((int32_t*)bcf_array)[idx]; } static void process_info(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { int i; for (i=0; in_info; i++) if ( line->d.info[i].key == fmt->id ) break; // output "." if the tag is not present if ( i==line->n_info ) { kputc('.', str); return; } bcf_info_t *info = &line->d.info[i]; // if this is a flag, output 1 if ( info->len <=0 ) { kputc('1', str); return; } if ( info->len == 1 ) { switch (info->type) { case BCF_BT_INT8: if ( info->v1.i==bcf_int8_missing ) kputc('.', str); else kputw(info->v1.i, str); break; case BCF_BT_INT16: if ( info->v1.i==bcf_int16_missing ) kputc('.', str); else kputw(info->v1.i, str); break; case BCF_BT_INT32: if ( info->v1.i==bcf_int32_missing ) kputc('.', str); else kputw(info->v1.i, str); break; case BCF_BT_FLOAT: if ( bcf_float_is_missing(info->v1.f) ) kputc('.', str); else ksprintf(str, "%g", info->v1.f); break; case BCF_BT_CHAR: kputc(info->v1.i, str); break; default: fprintf(stderr,"todo: type %d\n", info->type); exit(1); break; } } else if ( fmt->subscript >=0 ) { if ( info->len <= fmt->subscript ) { kputc('.', str); return; } #define BRANCH(type_t, is_missing, is_vector_end, kprint) { \ type_t val = ((type_t *) info->vptr)[fmt->subscript]; \ if ( is_missing || is_vector_end ) kputc('.',str); \ else kprint; \ } switch (info->type) { case BCF_BT_INT8: BRANCH(int8_t, val==bcf_int8_missing, val==bcf_int8_vector_end, kputw(val, str)); break; case BCF_BT_INT16: BRANCH(int16_t, val==bcf_int16_missing, val==bcf_int16_vector_end, kputw(val, str)); break; case BCF_BT_INT32: BRANCH(int32_t, val==bcf_int32_missing, val==bcf_int32_vector_end, kputw(val, str)); break; case BCF_BT_FLOAT: BRANCH(float, bcf_float_is_missing(val), bcf_float_is_vector_end(val), ksprintf(str, "%g", val)); break; default: fprintf(stderr,"todo: type %d\n", info->type); exit(1); break; } #undef BRANCH } else bcf_fmt_array(str, info->len, info->type, info->vptr); } static void init_format(convert_t *convert, bcf1_t *line, fmt_t *fmt) { fmt->id = bcf_hdr_id2int(convert->header, BCF_DT_ID, fmt->key); if ( fmt->id==-1 ) error("Error: no such tag defined in the VCF header: FORMAT/%s\n", fmt->key); fmt->fmt = NULL; int i; for (i=0; i<(int)line->n_fmt; i++) if ( line->d.fmt[i].id==fmt->id ) { fmt->fmt = &line->d.fmt[i]; break; } fmt->ready = 1; } static void process_format(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { if ( !fmt->ready ) init_format(convert, line, fmt); if ( fmt->fmt==NULL ) { kputc('.', str); return; } else if ( fmt->subscript >=0 ) { if ( fmt->fmt->n <= fmt->subscript ) { kputc('.', str); return; } if ( fmt->fmt->type == BCF_BT_FLOAT ) ksprintf(str, "%g", ((float*)(fmt->fmt->p + isample*fmt->fmt->size))[fmt->subscript]); else if ( fmt->fmt->type != BCF_BT_CHAR ) kputw(bcf_array_ivalue(fmt->fmt->p+isample*fmt->fmt->size,fmt->fmt->type,fmt->subscript), str); else error("TODO: %s:%d .. fmt->type=%d\n", __FILE__,__LINE__, fmt->fmt->type); } else bcf_fmt_array(str, fmt->fmt->n, fmt->fmt->type, fmt->fmt->p + isample*fmt->fmt->size); } static void process_gt(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { if ( !fmt->ready ) init_format(convert, line, fmt); if ( fmt->fmt==NULL ) { kputc('.', str); return; } bcf_format_gt(fmt->fmt, isample, str); } static void process_tgt(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { if ( !fmt->ready ) init_format(convert, line, fmt); if ( fmt->fmt==NULL ) { kputc('.', str); return; } assert( fmt->fmt->type==BCF_BT_INT8 ); int l; int8_t *x = (int8_t*)(fmt->fmt->p + isample*fmt->fmt->size); // FIXME: does not work with n_alt >= 64 for (l = 0; l < fmt->fmt->n && x[l] != bcf_int8_vector_end; ++l) { if (l) kputc("/|"[x[l]&1], str); if (x[l]>>1) { int ial = (x[l]>>1) - 1; kputs(line->d.allele[ial], str); } else kputc('.', str); } if (l == 0) kputc('.', str); } static void init_format_iupac(convert_t *convert, bcf1_t *line, fmt_t *fmt) { init_format(convert, line, fmt); if ( fmt->fmt==NULL ) return; // Init mapping between alleles and IUPAC table hts_expand(uint8_t, line->n_allele, convert->ndat, convert->dat); int8_t *dat = (int8_t*)convert->dat; int i; for (i=0; in_allele; i++) { if ( line->d.allele[i][1] ) dat[i] = -1; else { switch (line->d.allele[i][0]) { case 'A': dat[i] = 0; break; case 'C': dat[i] = 1; break; case 'G': dat[i] = 2; break; case 'T': dat[i] = 3; break; case 'a': dat[i] = 0; break; case 'c': dat[i] = 1; break; case 'g': dat[i] = 2; break; case 't': dat[i] = 3; break; default: dat[i] = -1; } } } } static void process_iupac_gt(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { if ( !fmt->ready ) init_format_iupac(convert, line, fmt); if ( fmt->fmt==NULL ) { kputc('.', str); return; } assert( fmt->fmt->type==BCF_BT_INT8 ); static const char iupac[4][4] = { {'A','M','R','W'},{'M','C','S','Y'},{'R','S','G','K'},{'W','Y','K','T'} }; int8_t *dat = (int8_t*)convert->dat; int8_t *x = (int8_t*)(fmt->fmt->p + isample*fmt->fmt->size); // FIXME: does not work with n_alt >= 64 int l = 0; while ( lfmt->n && x[l]!=bcf_int8_vector_end && x[l]!=bcf_int8_missing ) l++; if ( l==2 ) { // diploid int ia = (x[0]>>1) - 1, ib = (x[1]>>1) - 1; if ( ia>=0 && ian_allele && ib>=0 && ibn_allele && dat[ia]>=0 && dat[ib]>=0 ) { kputc(iupac[dat[ia]][dat[ib]], str); return; } } for (l = 0; l < fmt->fmt->n && x[l] != bcf_int8_vector_end; ++l) { if (l) kputc("/|"[x[l]&1], str); if (x[l]>>1) { int ial = (x[l]>>1) - 1; kputs(line->d.allele[ial], str); } else kputc('.', str); } if (l == 0) kputc('.', str); } static void process_sample(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { kputs(convert->header->samples[isample], str); } static void process_sep(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { if (fmt->key) kputs(fmt->key, str); } static void process_is_ts(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { int is_ts = 0; if ( bcf_get_variant_types(line) & (VCF_SNP|VCF_MNP) ) is_ts = abs(bcf_acgt2int(*line->d.allele[0])-bcf_acgt2int(*line->d.allele[1])) == 2 ? 1 : 0; kputc(is_ts ? '1' : '0', str); } static void process_type(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { int line_type = bcf_get_variant_types(line); int i = 0; if ( line_type == VCF_REF ) { kputs("REF", str); i++; } if ( line_type & VCF_SNP ) { if (i) kputc(',',str); kputs("SNP", str); i++; } if ( line_type & VCF_MNP ) { if (i) kputc(',',str); kputs("MNP", str); i++; } if ( line_type & VCF_INDEL ) { if (i) kputc(',',str); kputs("INDEL", str); i++; } if ( line_type & VCF_OTHER ) { if (i) kputc(',',str); kputs("OTHER", str); i++; } } static void process_line(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { vcf_format1(convert->header, line, str); } static void process_chrom_pos_id(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { if ( line->d.id[0]!='.' || line->d.id[1] ) { // ID is present kputs(line->d.id, str); } else { // use CHROM:POS instead of ID kputs(convert->header->id[BCF_DT_CTG][line->rid].key, str); kputc(':', str); kputw(line->pos+1, str); } } static void process_gt_to_prob3(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { int m,n,i; m = convert->ndat / sizeof(int32_t); n = bcf_get_genotypes(convert->header,line,&convert->dat,&m); convert->ndat = m * sizeof(int32_t); if ( n<=0 ) { // Throw an error or silently proceed? // // for (i=0; insamples; i++) kputs(" 0.33 0.33 0.33", str); // return; error("Error parsing GT tag at %s:%d\n", bcf_seqname(convert->header,line),line->pos+1); } n /= convert->nsamples; for (i=0; insamples; i++) { int32_t *ptr = (int32_t*)convert->dat + i*n; int j; for (j=0; jndat / sizeof(int32_t); n = bcf_get_format_int32(convert->header,line,"PL",&convert->dat,&m); convert->ndat = m * sizeof(int32_t); if ( n<=0 ) { // Throw an error or silently proceed? // // for (i=0; insamples; i++) kputs(" 0.33 0.33 0.33", str); // return; error("Error parsing PL tag at %s:%d\n", bcf_seqname(convert->header,line),line->pos+1); } n /= convert->nsamples; for (i=0; insamples; i++) { int32_t *ptr = (int32_t*)convert->dat + i*n; int j; float sum = 0; for (j=0; jn_allele ) { // haploid kputc(' ',str); ksprintf(str,"%f",pow(10,-0.1*ptr[0])/sum); kputs(" 0 ", str); ksprintf(str,"%f",pow(10,-0.1*ptr[1])/sum); } else { // diploid kputc(' ',str); ksprintf(str,"%f",pow(10,-0.1*ptr[0])/sum); kputc(' ',str); ksprintf(str,"%f",pow(10,-0.1*ptr[1])/sum); kputc(' ',str); ksprintf(str,"%f",pow(10,-0.1*ptr[2])/sum); } } } static void process_gp_to_prob3(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { int m,n,i; m = convert->ndat / sizeof(float); n = bcf_get_format_float(convert->header,line,"GP",&convert->dat,&m); convert->ndat = m * sizeof(float); if ( n<=0 ) { // Throw an error or silently proceed? // // for (i=0; insamples; i++) kputs(" 0.33 0.33 0.33", str); // return; error("Error parsing GP tag at %s:%d\n", bcf_seqname(convert->header,line),line->pos+1); } n /= convert->nsamples; for (i=0; insamples; i++) { float sum = 0, *ptr = (float*)convert->dat + i*n; int j; for (j=0; j1 ) error("[%s:%d:%f] GP value outside range [0,1]; bcftools convert expects the VCF4.3+ spec for the GP field encoding genotype posterior probabilities", bcf_seqname(convert->header,line),line->pos+1,ptr[j]); sum+=ptr[j]; } if ( j==line->n_allele ) ksprintf(str," %f %f %f",ptr[0],0.,ptr[1]); // haploid else ksprintf(str," %f %f %f",ptr[0],ptr[1],ptr[2]); // diploid } } static void process_gt_to_hap(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { // https://mathgen.stats.ox.ac.uk/impute/impute_v2.html#-known_haps_g // File containing known haplotypes for the study cohort. The format // is the same as the output format from IMPUTE2's -phase option: // five header columns (as in the -g file) followed by two columns // (haplotypes) per individual. Allowed values in the haplotype // columns are 0, 1, and ?. // If your study dataset is fully phased, you can replace the -g file // with a -known_haps_g file. This will cause IMPUTE2 to perform // haploid imputation, although it will still report diploid imputation // probabilities in the main output file. If any genotypes are missing, // they can be marked as '? ?' (two question marks separated by one // space) in the input file. (The program does not allow just one // allele from a diploid genotype to be missing.) If the reference // panels are also phased, IMPUTE2 will perform a single, fast // imputation step rather than its standard MCMC module this is how // the program imputes into pre-phased GWAS haplotypes. // The -known_haps_g file can also be used to specify study // genotypes that are "partially" phased, in the sense that some // genotypes are phased relative to a fixed reference point while // others are not. We anticipate that this will be most useful when // trying to phase resequencing data onto a scaffold of known // haplotypes. To mark a known genotype as unphased, place an // asterisk immediately after each allele, with no space between // the allele (0/1) and the asterisk (*); e.g., "0* 1*" for a // heterozygous genotype of unknown phase. int m, n, i; m = convert->ndat / sizeof(int32_t); n = bcf_get_genotypes(convert->header, line, &convert->dat, &m); convert->ndat = m * sizeof(int32_t); if ( n<=0 ) { // Throw an error or silently proceed? // // for (i=0; insamples; i++) kputs(" ...", str); // return; error("Error parsing GT tag at %s:%d\n", bcf_seqname(convert->header, line), line->pos+1); } n /= convert->nsamples; for (i=0; insamples; i++) { int32_t *ptr = (int32_t*)convert->dat + i*n; int j; for (j=0; j0) kputs(" ", str); // no space separation for first column if ( j==2 ) { // diploid if ( bcf_gt_is_missing(ptr[0]) || bcf_gt_is_missing(ptr[1]) ) { kputs("? ?", str); } else if ( bcf_gt_is_phased(ptr[1])) { ksprintf(str, "%d %d", bcf_gt_allele(ptr[0]), bcf_gt_allele(ptr[1])); } else { ksprintf(str, "%d* %d*", bcf_gt_allele(ptr[0]), bcf_gt_allele(ptr[1])); } } else if ( j==1 ) { // haploid if ( bcf_gt_is_missing(ptr[0]) ) kputs("? -", str); else if ( bcf_gt_allele(ptr[0])==1 ) kputs("1 -", str); // first ALT allele else kputs("0 -", str); // REF or something else than first ALT } else error("FIXME: not ready for ploidy %d\n", j); } } static void process_gt_to_hap2(convert_t *convert, bcf1_t *line, fmt_t *fmt, int isample, kstring_t *str) { // same as process_gt_to_hap but converts haploid genotypes into diploid int m, n, i; m = convert->ndat / sizeof(int32_t); n = bcf_get_genotypes(convert->header, line, &convert->dat, &m); convert->ndat = m * sizeof(int32_t); if ( n<=0 ) error("Error parsing GT tag at %s:%d\n", bcf_seqname(convert->header, line), line->pos+1); n /= convert->nsamples; for (i=0; insamples; i++) { int32_t *ptr = (int32_t*)convert->dat + i*n; int j; for (j=0; j0) kputs(" ", str); // no space separation for first column if ( j==2 ) { // diploid if ( bcf_gt_is_missing(ptr[0]) || bcf_gt_is_missing(ptr[1]) ) { kputs("? ?", str); } else if ( bcf_gt_is_phased(ptr[1])) { ksprintf(str, "%d %d", bcf_gt_allele(ptr[0]), bcf_gt_allele(ptr[1])); } else { ksprintf(str, "%d* %d*", bcf_gt_allele(ptr[0]), bcf_gt_allele(ptr[1])); } } else if ( j==1 ) { // haploid if ( bcf_gt_is_missing(ptr[0]) ) kputs("? ?", str); else if ( bcf_gt_allele(ptr[0])==1 ) kputs("1 1", str); // first ALT allele else kputs("0 0", str); // REF or something else than first ALT } else error("FIXME: not ready for ploidy %d\n", j); } } static fmt_t *register_tag(convert_t *convert, int type, char *key, int is_gtf) { convert->nfmt++; if ( convert->nfmt > convert->mfmt ) { convert->mfmt += 10; convert->fmt = (fmt_t*) realloc(convert->fmt, convert->mfmt*sizeof(fmt_t)); } fmt_t *fmt = &convert->fmt[ convert->nfmt-1 ]; fmt->type = type; fmt->key = key ? strdup(key) : NULL; fmt->is_gt_field = is_gtf; fmt->subscript = -1; // Allow non-format tags, such as CHROM, INFO, etc., to appear amongst the format tags. if ( key ) { int id = bcf_hdr_id2int(convert->header, BCF_DT_ID, key); if ( fmt->type==T_FORMAT && !bcf_hdr_idinfo_exists(convert->header,BCF_HL_FMT,id) ) { if ( !strcmp("CHROM",key) ) { fmt->type = T_CHROM; } else if ( !strcmp("POS",key) ) { fmt->type = T_POS; } else if ( !strcmp("ID",key) ) { fmt->type = T_ID; } else if ( !strcmp("REF",key) ) { fmt->type = T_REF; } else if ( !strcmp("ALT",key) ) { fmt->type = T_ALT; } else if ( !strcmp("FIRST_ALT",key) ) { fmt->type = T_FIRST_ALT; } else if ( !strcmp("QUAL",key) ) { fmt->type = T_QUAL; } else if ( !strcmp("FILTER",key) ) { fmt->type = T_FILTER; } else if ( !strcmp("_CHROM_POS_ID",key) ) { fmt->type = T_CHROM_POS_ID; } else if ( id>=0 && bcf_hdr_idinfo_exists(convert->header,BCF_HL_INFO,id) ) { fmt->type = T_INFO; fprintf(stderr,"Warning: Assuming INFO/%s\n", key); } } } switch (fmt->type) { case T_FIRST_ALT: fmt->handler = &process_first_alt; break; case T_CHROM_POS_ID: fmt->handler = &process_chrom_pos_id; break; case T_GT_TO_PROB3: fmt->handler = &process_gt_to_prob3; break; case T_PL_TO_PROB3: fmt->handler = &process_pl_to_prob3; break; case T_GP_TO_PROB3: fmt->handler = &process_gp_to_prob3; break; case T_CHROM: fmt->handler = &process_chrom; break; case T_POS: fmt->handler = &process_pos; break; case T_ID: fmt->handler = &process_id; break; case T_REF: fmt->handler = &process_ref; break; case T_ALT: fmt->handler = &process_alt; break; case T_QUAL: fmt->handler = &process_qual; break; case T_FILTER: fmt->handler = &process_filter; convert->max_unpack |= BCF_UN_FLT; break; case T_INFO: fmt->handler = &process_info; convert->max_unpack |= BCF_UN_INFO; break; case T_FORMAT: fmt->handler = &process_format; convert->max_unpack |= BCF_UN_FMT; break; case T_SAMPLE: fmt->handler = &process_sample; break; case T_SEP: fmt->handler = &process_sep; break; case T_IS_TS: fmt->handler = &process_is_ts; break; case T_TYPE: fmt->handler = &process_type; break; case T_MASK: fmt->handler = NULL; break; case T_GT: fmt->handler = &process_gt; convert->max_unpack |= BCF_UN_FMT; break; case T_TGT: fmt->handler = &process_tgt; convert->max_unpack |= BCF_UN_FMT; break; case T_IUPAC_GT: fmt->handler = &process_iupac_gt; convert->max_unpack |= BCF_UN_FMT; break; case T_GT_TO_HAP: fmt->handler = &process_gt_to_hap; convert->max_unpack |= BCF_UN_FMT; break; case T_GT_TO_HAP2: fmt->handler = &process_gt_to_hap2; convert->max_unpack |= BCF_UN_FMT; break; case T_LINE: fmt->handler = &process_line; break; default: error("TODO: handler for type %d\n", fmt->type); } if ( key ) { if ( fmt->type==T_INFO ) { fmt->id = bcf_hdr_id2int(convert->header, BCF_DT_ID, key); if ( fmt->id==-1 ) error("Error: no such tag defined in the VCF header: INFO/%s\n", key); } } return fmt; } static int parse_subscript(char **p) { char *q = *p; if ( *q!='{' ) return -1; q++; while ( *q && *q!='}' && isdigit(*q) ) q++; if ( *q!='}' ) return -1; int idx = atoi((*p)+1); *p = q+1; return idx; } static char *parse_tag(convert_t *convert, char *p, int is_gtf) { char *q = ++p; while ( *q && (isalnum(*q) || *q=='_' || *q=='.') ) q++; kstring_t str = {0,0,0}; if ( q-p==0 ) error("Could not parse format string: %s\n", convert->format_str); kputsn(p, q-p, &str); if ( is_gtf ) { if ( !strcmp(str.s, "SAMPLE") ) register_tag(convert, T_SAMPLE, "SAMPLE", is_gtf); else if ( !strcmp(str.s, "GT") ) register_tag(convert, T_GT, "GT", is_gtf); else if ( !strcmp(str.s, "TGT") ) register_tag(convert, T_TGT, "GT", is_gtf); else if ( !strcmp(str.s, "IUPACGT") ) register_tag(convert, T_IUPAC_GT, "GT", is_gtf); else { fmt_t *fmt = register_tag(convert, T_FORMAT, str.s, is_gtf); fmt->subscript = parse_subscript(&q); } } else { if ( !strcmp(str.s, "CHROM") ) register_tag(convert, T_CHROM, str.s, is_gtf); else if ( !strcmp(str.s, "POS") ) register_tag(convert, T_POS, str.s, is_gtf); else if ( !strcmp(str.s, "ID") ) register_tag(convert, T_ID, str.s, is_gtf); else if ( !strcmp(str.s, "REF") ) register_tag(convert, T_REF, str.s, is_gtf); else if ( !strcmp(str.s, "ALT") ) { fmt_t *fmt = register_tag(convert, T_ALT, str.s, is_gtf); fmt->subscript = parse_subscript(&q); } else if ( !strcmp(str.s, "FIRST_ALT") ) register_tag(convert, T_FIRST_ALT, str.s, is_gtf); else if ( !strcmp(str.s, "QUAL") ) register_tag(convert, T_QUAL, str.s, is_gtf); else if ( !strcmp(str.s, "FILTER") ) register_tag(convert, T_FILTER, str.s, is_gtf); else if ( !strcmp(str.s, "QUAL") ) register_tag(convert, T_QUAL, str.s, is_gtf); else if ( !strcmp(str.s, "IS_TS") ) register_tag(convert, T_IS_TS, str.s, is_gtf); else if ( !strcmp(str.s, "TYPE") ) register_tag(convert, T_TYPE, str.s, is_gtf); else if ( !strcmp(str.s, "MASK") ) register_tag(convert, T_MASK, str.s, is_gtf); else if ( !strcmp(str.s, "LINE") ) register_tag(convert, T_LINE, str.s, is_gtf); else if ( !strcmp(str.s, "_CHROM_POS_ID") ) register_tag(convert, T_CHROM_POS_ID, str.s, is_gtf); else if ( !strcmp(str.s, "_GT_TO_PROB3") ) register_tag(convert, T_GT_TO_PROB3, str.s, is_gtf); else if ( !strcmp(str.s, "_PL_TO_PROB3") ) register_tag(convert, T_PL_TO_PROB3, str.s, is_gtf); else if ( !strcmp(str.s, "_GP_TO_PROB3") ) register_tag(convert, T_GP_TO_PROB3, str.s, is_gtf); else if ( !strcmp(str.s, "_GT_TO_HAP") ) register_tag(convert, T_GT_TO_HAP, str.s, is_gtf); else if ( !strcmp(str.s, "_GT_TO_HAP2") ) register_tag(convert, T_GT_TO_HAP2, str.s, is_gtf); else if ( !strcmp(str.s, "INFO") ) { if ( *q!='/' ) error("Could not parse format string: %s\n", convert->format_str); p = ++q; str.l = 0; while ( *q && (isalnum(*q) || *q=='_' || *q=='.') ) q++; if ( q-p==0 ) error("Could not parse format string: %s\n", convert->format_str); kputsn(p, q-p, &str); fmt_t *fmt = register_tag(convert, T_INFO, str.s, is_gtf); fmt->subscript = parse_subscript(&q); } else { fmt_t *fmt = register_tag(convert, T_INFO, str.s, is_gtf); fmt->subscript = parse_subscript(&q); } } free(str.s); return q; } static char *parse_sep(convert_t *convert, char *p, int is_gtf) { char *q = p; kstring_t str = {0,0,0}; while ( *q && *q!='[' && *q!=']' && *q!='%' ) { if ( *q=='\\' ) { q++; if ( *q=='n' ) kputc('\n', &str); else if ( *q=='t' ) kputc('\t', &str); else kputc(*q, &str); } else kputc(*q, &str); q++; } if ( !str.l ) error("Could not parse format string: %s\n", convert->format_str); register_tag(convert, T_SEP, str.s, is_gtf); free(str.s); return q; } convert_t *convert_init(bcf_hdr_t *hdr, int *samples, int nsamples, const char *format_str) { convert_t *convert = (convert_t*) calloc(1,sizeof(convert_t)); convert->header = hdr; convert->format_str = strdup(format_str); int i, is_gtf = 0; char *p = convert->format_str; while ( *p ) { //fprintf(stderr,"<%s>\n", p); switch (*p) { case '[': is_gtf = 1; p++; break; case ']': is_gtf = 0; register_tag(convert, T_SEP, NULL, 0); p++; break; case '%': p = parse_tag(convert, p, is_gtf); break; default: p = parse_sep(convert, p, is_gtf); break; } } if ( nsamples ) { convert->nsamples = nsamples; convert->samples = (int*) malloc(sizeof(int)*nsamples); for (i=0; insamples; i++) convert->samples[i] = samples[i]; } else { convert->nsamples = bcf_hdr_nsamples(convert->header); convert->samples = (int*) malloc(sizeof(int)*convert->nsamples); for (i=0; insamples; i++) convert->samples[i] = i; } return convert; } void convert_destroy(convert_t *convert) { int i; for (i=0; infmt; i++) if ( convert->fmt[i].key ) free(convert->fmt[i].key); if ( convert->mfmt ) free(convert->fmt); free(convert->dat); free(convert->samples); free(convert->format_str); free(convert); } int convert_header(convert_t *convert, kstring_t *str) { int i, icol = 0, l_ori = str->l; bcf_hdr_t *hdr = convert->header; // Supress the header output if LINE is present for (i=0; infmt; i++) if ( convert->fmt[i].type == T_LINE ) break; if ( i!=convert->nfmt ) return str->l - l_ori; kputs("# ", str); for (i=0; infmt; i++) { // Genotype fields if ( convert->fmt[i].is_gt_field ) { int j = i, js, k; while ( convert->fmt[j].is_gt_field ) j++; for (js=0; jsnsamples; js++) { int ks = convert->samples[js]; for (k=i; kfmt[k].type == T_SEP ) { if ( convert->fmt[k].key ) kputs(convert->fmt[k].key, str); } else if ( convert->fmt[k].type == T_SAMPLE ) ksprintf(str, "[%d]%s", ++icol, convert->fmt[k].key); else ksprintf(str, "[%d]%s:%s", ++icol, hdr->samples[ks], convert->fmt[k].key); } } i = j-1; continue; } // Fixed fields if ( convert->fmt[i].type == T_SEP ) { if ( convert->fmt[i].key ) kputs(convert->fmt[i].key, str); continue; } ksprintf(str, "[%d]%s", ++icol, convert->fmt[i].key); } return str->l - l_ori; } int convert_line(convert_t *convert, bcf1_t *line, kstring_t *str) { int l_ori = str->l; bcf_unpack(line, convert->max_unpack); int i, ir; str->l = 0; for (i=0; infmt; i++) { // Genotype fields if ( convert->fmt[i].is_gt_field ) { int j = i, js, k; while ( convert->fmt[j].is_gt_field ) { convert->fmt[j].ready = 0; j++; } for (js=0; jsnsamples; js++) { int ks = convert->samples[js]; for (k=i; kfmt[k].type == T_MASK ) { for (ir=0; irnreaders; ir++) kputc(bcf_sr_has_line(convert->readers,ir)?'1':'0', str); } else if ( convert->fmt[k].handler ) convert->fmt[k].handler(convert, line, &convert->fmt[k], ks, str); } } i = j-1; continue; } // Fixed fields if ( convert->fmt[i].type == T_MASK ) { for (ir=0; irnreaders; ir++) kputc(bcf_sr_has_line(convert->readers,ir)?'1':'0', str); } else if ( convert->fmt[i].handler ) convert->fmt[i].handler(convert, line, &convert->fmt[i], -1, str); } return str->l - l_ori; } bcftools-1.2/convert.h000066400000000000000000000030361246371514100150260ustar00rootroot00000000000000/* convert.h -- functions for converting between VCF/BCF and related formats. Copyright (C) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #ifndef __CONVERT_H__ #define __CONVERT_H__ #include typedef struct _convert_t convert_t; convert_t *convert_init(bcf_hdr_t *hdr, int *samples, int nsamples, const char *str); void convert_destroy(convert_t *convert); int convert_header(convert_t *convert, kstring_t *str); int convert_line(convert_t *convert, bcf1_t *rec, kstring_t *str); #endif bcftools-1.2/doc/000077500000000000000000000000001246371514100137405ustar00rootroot00000000000000bcftools-1.2/doc/bcftools.1000066400000000000000000002212401246371514100156360ustar00rootroot00000000000000'\" t .\" Title: bcftools .\" Author: [see the "AUTHORS" section] .\" Generator: DocBook XSL Stylesheets v1.76.1 .\" Date: 2015-01-21 15:01 GMT .\" Manual: \ \& .\" Source: \ \& .\" Language: English .\" .TH "BCFTOOLS" "1" "2015\-01\-21 15:01 GMT" "\ \&" "\ \&" .\" ----------------------------------------------------------------- .\" * Define some portability stuff .\" ----------------------------------------------------------------- .\" ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ .\" http://bugs.debian.org/507673 .\" http://lists.gnu.org/archive/html/groff/2009-02/msg00013.html .\" ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ .ie \n(.g .ds Aq \(aq .el .ds Aq ' .\" ----------------------------------------------------------------- .\" * set default formatting .\" ----------------------------------------------------------------- .\" disable hyphenation .nh .\" disable justification (adjust text to left margin only) .ad l .\" ----------------------------------------------------------------- .\" * MAIN CONTENT STARTS HERE * .\" ----------------------------------------------------------------- .SH "NAME" bcftools \- utilities for variant calling and manipulating VCFs and BCFs\&. .SH "SYNOPSIS" .sp \fBbcftools\fR [\fICOMMAND\fR] [\fIOPTIONS\fR] .SH "DESCRIPTION" .sp BCFtools is a set of utilities that manipulate variant calls in the Variant Call Format (VCF) and its binary counterpart BCF\&. All commands work transparently with both VCFs and BCFs, both uncompressed and BGZF\-compressed\&. .sp Most commands accept VCF, bgzipped VCF and BCF with filetype detected automatically even when streaming from a pipe\&. Indexed VCF and BCF will work in all situations\&. Un\-indexed VCF and BCF and streams will work in most, but not all situations\&. .sp BCFtools is designed to work on a stream\&. It regards an input file "\-" as the standard input (stdin) and outputs to the standard output (stdout)\&. Several commands can thus be combined with Unix pipes\&. .SS "VERSION" .sp This manual page was last updated \fB2015\-01\-21 15:01 GMT\fR and refers to bcftools git version \fB1\&.1\-140\-g9b0e7cc+\fR\&. .SS "BCF1" .sp The BCF1 format output by versions of samtools <= 0\&.1\&.19 is \fBnot\fR compatible with this version of bcftools\&. To read BCF1 files one can use the view command from old versions of bcftools packaged with samtools versions <= 0\&.1\&.19 to convert to VCF, which can then be read by this version of bcftools\&. .sp .if n \{\ .RS 4 .\} .nf samtools\-0\&.1\&.19/bcftools/bcftools view file\&.bcf1 | bcftools view .fi .if n \{\ .RE .\} .SS "VARIANT CALLING" .sp See \fIbcftools call\fR for variant calling from the output of the \fIsamtools mpileup\fR command\&. In versions of samtools <= 0\&.1\&.19 calling was done with \fIbcftools view\fR\&. Users are now required to choose between the old samtools calling model (\fI\-c/\-\-consensus\-caller\fR) and the new multiallelic calling model (\fI\-m/\-\-multiallelic\-caller\fR)\&. The multiallelic calling model is recommended for most tasks\&. .SH "LIST OF COMMANDS" .sp For a full list of available commands, run \fBbcftools\fR without arguments\&. For a full list of available options, run \fBbcftools\fR \fICOMMAND\fR without arguments\&. .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} \fBannotate\fR \&.\&. edit VCF files, add or remove annotations .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} \fBcall\fR \&.\&. SNP/indel calling (former "view") .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} \fBconcat\fR \&.\&. concatenate VCF/BCF files from the same set of samples .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} \fBconsensus\fR \&.\&. create consensus sequence by applying VCF variants .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} \fBconvert\fR \&.\&. convert VCF/BCF to other formats and back .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} \fBfilter\fR \&.\&. filter VCF/BCF files using fixed thresholds .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} \fBgtcheck\fR \&.\&. check sample concordance, detect sample swaps and contamination .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} \fBindex\fR \&.\&. index VCF/BCF .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} \fBisec\fR \&.\&. intersections of VCF/BCF files .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} \fBmerge\fR \&.\&. merge VCF/BCF files files from non\-overlapping sample sets .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} \fBnorm\fR \&.\&. normalize indels .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} \fBplugin\fR \&.\&. run user\-defined plugin .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} \fBquery\fR \&.\&. transform VCF/BCF into user\-defined formats .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} \fBreheader\fR \&.\&. modify VCF/BCF header, change sample names .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} \fBroh\fR \&.\&. identify runs of homo/auto\-zygosity .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} \fBstats\fR \&.\&. produce VCF/BCF stats (former vcfcheck) .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} \fBview\fR \&.\&. subset, filter and convert VCF and BCF files .RE .SH "LIST OF SCRIPTS" .sp Some helper scripts are bundled with the bcftools code\&. .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} \fBplot\-vcfstats\fR \&.\&. plots the output of \fBstats\fR .RE .SH "COMMANDS AND OPTIONS" .SS "Common Options" .sp The following options are common to many bcftools commands\&. See usage for specific commands to see if they apply\&. .PP \fIFILE\fR .RS 4 Files can be both VCF or BCF, uncompressed or BGZF\-compressed\&. The file "\-" is interpreted as standard input\&. Some tools may require tabix\- or CSI\-indexed files\&. .RE .PP \fB\-c, \-\-collapse\fR \fIsnps\fR|\fIindels\fR|\fIboth\fR|\fIall\fR|\fIsome\fR|\fInone\fR|\fIid\fR .RS 4 Controls how to treat records with duplicate positions and defines compatible records across multiple input files\&. Here by "compatible" we mean records which should be considered as identical by the tools\&. For example, when performing line intersections, the desire may be to consider as identical all sites with matching positions (\fBbcftools isec \-c\fR \fIall\fR), or only sites with matching variant type (\fBbcftools isec \-c\fR \fIsnps\fR\ \& \fB\-c\fR \fIindels\fR), or only sites with all alleles identical (\fBbcftools isec \-c\fR \fInone\fR)\&. .PP \fInone\fR .RS 4 only records with identical REF and ALT alleles are compatible .RE .PP \fIsome\fR .RS 4 only records where some subset of ALT alleles match are compatible .RE .PP \fIall\fR .RS 4 all records are compatible, regardless of whether the ALT alleles match or not\&. In the case of records with the same position, only the first will be considered and appear on output\&. .RE .PP \fIsnps\fR .RS 4 any SNP records are compatible, regardless of whether the ALT alleles match or not\&. For duplicate positions, only the first SNP record will be considered and appear on output\&. .RE .PP \fIindels\fR .RS 4 all indel records are compatible, regardless of whether the REF and ALT alleles match or not\&. For duplicate positions, only the first indel record will be considered and appear on output\&. .RE .PP \fIboth\fR .RS 4 abbreviation of "\fB\-c\fR \fIindels\fR\ \& \fB\-c\fR \fIsnps\fR" .RE .PP \fIid\fR .RS 4 only records with identical ID column are compatible\&. Supported by \fBbcftools merge\fR only\&. .RE .RE .PP \fB\-f, \-\-apply\-filters\fR \fILIST\fR .RS 4 Skip sites where FILTER column does not contain any of the strings listed in \fILIST\fR\&. For example, to include only sites which have no filters set, use \fB\-f\fR \fI\&.,PASS\fR\&. .RE .PP \fB\-o, \-\-output\fR \fIFILE\fR .RS 4 When output consists of a single stream, write it to \fIFILE\fR rather than to standard output, where it is written by default\&. .RE .PP \fB\-O, \-\-output\-type\fR \fIb\fR|\fIu\fR|\fIz\fR|\fIv\fR .RS 4 Output compressed BCF (\fIb\fR), uncompressed BCF (\fIu\fR), compressed VCF (\fIz\fR), uncompressed VCF (\fIv\fR)\&. .RE .PP \fB\-r, \-\-regions\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 Comma\-separated list of regions, see also \fB\-R, \-\-regions\-file\fR\&. Note that \fB\-r\fR cannot be used in combination with \fB\-R\fR\&. .RE .PP \fB\-R, \-\-regions\-file\fR \fIFILE\fR .RS 4 Regions can be specified either on command line or in a VCF, BED, or tab\-delimited file (the default)\&. The columns of the tab\-delimited file are: CHROM, POS, and, optionally, POS_TO, where positions are 1\-based and inclusive\&. Uncompressed files are stored in memory, while bgzip\-compressed and tabix\-indexed region files are streamed\&. Note that sequence names must match exactly, "chr20" is not the same as "20"\&. Also note that chromosome ordering in \fIFILE\fR will be respected, the VCF will be processed in the order in which chromosomes first appear in \fIFILE\fR\&. However, within chromosomes, the VCF will always be processed in ascending genomic coordinate order no matter what order they appear in \fIFILE\fR\&. Note that overlapping regions in \fIFILE\fR can result in duplicated out of order positions in the output\&. This option requires indexed VCF/BCF files\&. Note that \fB\-R\fR cannot be used in combination with \fB\-r\fR\&. .RE .PP \fB\-s, \-\-samples\fR [^]\fILIST\fR .RS 4 Comma\-separated list of samples to include or exclude if prefixed with "^"\&. .RE .PP \fB\-S, \-\-samples\-file\fR \fIFILE\fR .RS 4 File of sample names to include or exclude if prefixed with "^"\&. One sample per line\&. The command \fBbcftools call\fR accepts an optional second column indicating ploidy (0, 1 or 2) and can parse also PED files\&. With \fBbcftools call\fR\fB \-C\fR \fItrio\fR, PED file is expected\&. .RE .PP \fB\-t, \-\-targets\fR [^]\fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 Similar as \fB\-r, \-\-regions\fR, but the next position is accessed by streaming the whole VCF/BCF rather than using the tbi/csi index\&. Both \fB\-r\fR and \fB\-t\fR options can be applied simultaneously: \fB\-r\fR uses the index to jump to a region and \fB\-t\fR discards positions which are not in the targets\&. Unlike \fB\-r\fR, targets can be prefixed with "^" to request logical complement\&. For example, "^X,Y,MT" indicates that sequences X, Y and MT should be skipped\&. Yet another difference between the two is that \fB\-r\fR checks both start and end positions of indels, whereas \fB\-t\fR checks start positions only\&. Note that \fB\-t\fR cannot be used in combination with \fB\-T\fR\&. .RE .PP \fB\-T, \-\-targets\-file\fR [^]\fIFILE\fR .RS 4 Same \fB\-t, \-\-targets\fR, but reads regions from a file\&. Note that \fB\-T\fR cannot be used in combination with \fB\-t\fR\&. .RE .PP .RS 4 With the \fBcall \-C\fR \fIalleles\fR command, third column of the targets file must be comma\-separated list of alleles, starting with the reference allele\&. Such a file can be easily created from a VCF using: .RE .sp .if n \{\ .RS 4 .\} .nf bcftools query \-f\*(Aq%CHROM\et%POS\et%REF,%ALT\en\*(Aq file\&.vcf .fi .if n \{\ .RE .\} .SS "bcftools annotate \fI[OPTIONS]\fR \fIFILE\fR" .sp This command allows to add or remove annotations\&. .PP \fB\-a, \-\-annotations\fR \fIfile\fR .RS 4 Bgzip\-compressed and tabix\-indexed file with annotations\&. The file can be VCF, BED, or a tab\-delimited file with mandatory columns CHROM, POS (or, alternatively, FROM and TO), optional columns REF and ALT, and arbitrary number of annotation columns\&. BED files are expected to have the "\&.bed" or "\&.bed\&.gz" suffix (case\-insensitive), otherwise a tab\-delimited file is assumed\&. Note that in case of tab\-delimited file, the coordinates POS, FROM and TO are one\-based and inclusive\&. When REF and ALT are present, only matching VCF records will be annotated\&. When multiple ALT alleles are present in the annotation file (given as comma\-separated list of alleles), at least one must match one of the alleles in the corresponding VCF record\&. Similarly, at least one alternate allele from a multi\-allelic VCF record must be present in the annotation file\&. Note that flag types, such as "INFO/FLAG", can be annotated by including a field with the value "1" to set the flag, "0" to remove it, or "\&." to keep existing flags\&. See also \fB\-c, \-\-columns\fR and \fB\-h, \-\-header\-lines\fR\&. .RE .sp .if n \{\ .RS 4 .\} .nf # Sample annotation file with columns CHROM, POS, STRING_TAG, NUMERIC_TAG 1 752566 SomeString 5 1 798959 SomeOtherString 6 # etc\&. .fi .if n \{\ .RE .\} .PP \fB\-c, \-\-columns\fR \fIlist\fR .RS 4 Comma\-separated list of columns or tags to carry over from the annotation file (see also \fB\-a, \-\-annotations\fR)\&. If the annotation file is not a VCF/BCF, \fIlist\fR describes the columns of the annotation file and must include CHROM, POS (or, alternatively, FROM and TO), and optionally REF and ALT\&. Unused columns which should be ignored can be indicated by "\-"\&. If the annotation file is a VCF/BCF, only the edited columns/tags must be present and their order does not matter\&. The columns ID, QUAL, FILTER, INFO and FORMAT can be edited, where INFO tags can be written both as "INFO/TAG" or simply "TAG", and FORMAT tags can be written as "FORMAT/TAG" or "FMT/TAG"\&. To carry over all INFO annotations, use "INFO"\&. To add all INFO annotations except "TAG", use "^INFO/TAG"\&. By default, existing values are replaced\&. To add values without overwriting existing annotations, use "+TAG" instead of "TAG"\&. To replace only existing values without modifying missing annotations, use "\-TAG"\&. If the annotation file is not a VCF/BCF, all new annotations must be defined via \fB\-h, \-\-header\-lines\fR\&. .RE .PP \fB\-e, \-\-exclude\fR \fIEXPRESSION\fR .RS 4 exclude sites for which \fIEXPRESSION\fR is true\&. For valid expressions see \fBEXPRESSIONS\fR\&. .RE .PP \fB\-h, \-\-header\-lines\fR \fIfile\fR .RS 4 Lines to append to the VCF header, see also \fB\-c, \-\-columns\fR and \fB\-a, \-\-annotations\fR\&. For example: .RE .sp .if n \{\ .RS 4 .\} .nf ##INFO= ##INFO= .fi .if n \{\ .RE .\} .PP \fB\-I, \-\-set\-id\fR [+]\fIFORMAT\fR .RS 4 assign ID on the fly\&. The format is the same as in the \fBquery\fR command (see below)\&. By default all existing IDs are replaced\&. If the format string is preceded by "+", only missing IDs will be set\&. For example, one can use .RE .sp .if n \{\ .RS 4 .\} .nf bcftools annotate \-\-set\-id +\*(Aq%CHROM\e_%POS\e_%REF\e_%FIRST_ALT\*(Aq file\&.vcf .fi .if n \{\ .RE .\} .PP \fB\-i, \-\-include\fR \fIEXPRESSION\fR .RS 4 include only sites for which \fIEXPRESSION\fR is true\&. For valid expressions see \fBEXPRESSIONS\fR\&. .RE .PP \fB\-o, \-\-output\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-O, \-\-output\-type\fR \fIb\fR|\fIu\fR|\fIz\fR|\fIv\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-r, \-\-regions\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-R, \-\-regions\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-\-rename\-chrs\fR \fIfile\fR .RS 4 rename chromosomes according to the map in \fIfile\fR, with "old_name new_name\en" pairs separated by whitespaces, each on a separate line\&. .RE .PP \fB\-s, \-\-samples\fR [^]\fILIST\fR .RS 4 subset of samples to annotate, see also \fBCommon Options\fR .RE .PP \fB\-S, \-\-samples\-file\fR \fIFILE\fR .RS 4 subset of samples to annotate\&. If the samples are named differently in the target VCF and the \fB\-a, \-\-annotations\fR VCF, the name mapping can be given as "src_name dst_name\en", separated by whitespaces, each pair on a separate line\&. .RE .PP \fB\-x, \-\-remove\fR \fIlist\fR .RS 4 List of annotations to remove\&. Use "FILTER" to remove all filters or "FILTER/SomeFilter" to remove a specific filter\&. Similarly, "INFO" can be used to remove all INFO tags and "FORMAT" to remove all FORMAT tags except GT\&. To remove all INFO tags except "FOO" and "BAR", use "^INFO/FOO,INFO/BAR" (and similarly for FORMAT and FILTER)\&. "INFO" can be abbreviated to "INF" and "FORMAT" to "FMT"\&. .RE .sp \fBExamples:\fR .sp .if n \{\ .RS 4 .\} .nf # Remove three fields bcftools annotate \-x ID,INFO/DP,FORMAT/DP file\&.vcf\&.gz # Remove all INFO fields and all FORMAT fields except for GT and PL bcftools annotate \-x INFO,^FORMAT/GT,FORMAT/PL file\&.vcf # Add ID, QUAL and INFO/TAG, not replacing TAG if already present bcftools annotate \-a src\&.bcf \-c ID,QUAL,+TAG dst\&.bcf # Carry over all INFO and FORMAT annotations except FORMAT/GT bcftools annotate \-a src\&.bcf \-c INFO,^FORMAT/GT dst\&.bcf # Annotate from a tab\-delimited file with six columns (the fifth is ignored), # first indexing with tabix\&. The coordinates are 1\-based\&. tabix \-s1 \-b2 \-e2 annots\&.tab\&.gz bcftools annotate \-a annots\&.tab\&.gz \-h annots\&.hdr \-c CHROM,POS,REF,ALT,\-,TAG file\&.vcf # Annotate from a tab\-delimited file with regions (1\-based coordinates, inclusive) tabix \-s1 \-b2 \-e3 annots\&.tab\&.gz bcftools annotate \-a annots\&.tab\&.gz \-h annots\&.hdr \-c CHROM,FROM,TO,TAG inut\&.vcf # Annotate from a bed file (0\-based coordinates, half\-closed, half\-open intervals) bcftools annotate \-a annots\&.bed\&.gz \-h annots\&.hdr \-c CHROM,FROM,TO,TAG input\&.vcf .fi .if n \{\ .RE .\} .SS "bcftools call \fI[OPTIONS]\fR \fIFILE\fR" .sp This command replaces the former \fBbcftools view\fR caller\&. Some of the original functionality has been temporarily lost in the process of transition under htslib, but will be added back on popular demand\&. The original calling model can be invoked with the \fB\-c\fR option\&. .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBFile format options:\fR .RS 4 .PP \fB\-O, \-\-output\-type\fR \fIb\fR|\fIu\fR|\fIz\fR|\fIv\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-r, \-\-regions\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-R, \-\-regions\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-s, \-\-samples\fR \fILIST\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-S, \-\-samples\-file\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-t, \-\-targets\fR \fILIST\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-T, \-\-targets\-file\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBInput/output options:\fR .RS 4 .PP \fB\-A, \-\-keep\-alts\fR .RS 4 output all alternate alleles present in the alignments even if they do not appear in any of the genotypes .RE .PP \fB\-f, \-\-format\-fields\fR \fIlist\fR .RS 4 comma\-separated list of FORMAT fields to output for each sample\&. Currently GQ and GP fields are supported\&. For convenience, the fields can be given as lower case letters\&. .RE .PP \fB\-g, \-\-gvcf\fR \fIINT\fR .RS 4 output also gVCF blocks of homozygous REF calls\&. The parameter \fIINT\fR is the minimum per\-sample depth required to include a site in the non\-variant block\&. .RE .PP \fB\-M, \-\-keep\-masked\-ref\fR .RS 4 output sites where REF allele is N .RE .PP \fB\-o, \-\-output\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-V, \-\-skip\-variants\fR \fIsnps\fR|\fIindels\fR .RS 4 skip indel/SNP sites .RE .PP \fB\-v, \-\-variants\-only\fR .RS 4 output variant sites only .RE .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBConsensus/variant calling options:\fR .RS 4 .PP \fB\-c, \-\-consensus\-caller\fR .RS 4 the original \fBsamtools\fR/\fBbcftools\fR calling method (conflicts with \fB\-m\fR) .RE .PP \fB\-C, \-\-constrain\fR \fIalleles\fR|\fItrio\fR .RS 4 .PP \fIalleles\fR .RS 4 call genotypes given alleles\&. See also \fB\-T, \-\-targets\-file\fR\&. .RE .PP \fItrio\fR .RS 4 call genotypes given the father\-mother\-child constraint\&. See also \fB\-s, \-\-samples\fR and \fB\-n, \-\-novel\-rate\fR\&. .RE .RE .PP \fB\-m, \-\-multiallelic\-caller\fR .RS 4 alternative modelfor multiallelic and rare\-variant calling designed to overcome known limitations in \fB\-c\fR calling model (conflicts with \fB\-c\fR) .RE .PP \fB\-n, \-\-novel\-rate\fR \fIfloat\fR[,\&...] .RS 4 likelihood of novel mutation for constrained \fB\-C\fR \fItrio\fR calling\&. The trio genotype calling maximizes likelihood of a particular combination of genotypes for father, mother and the child P(F=i,M=j,C=k) = P(unconstrained) * Pn + P(constrained) * (1\-Pn)\&. By providing three values, the mutation rate Pn is set explictly for SNPs, deletions and insertions, respectively\&. If two values are given, the first is interpreted as the mutation rate of SNPs and the second is used to calculate the mutation rate of indels according to their length as Pn=\fIfloat\fR*exp(\-a\-b*len), where a=22\&.8689, b=0\&.2994 for insertions and a=21\&.9313, b=0\&.2856 for deletions [pubmed:23975140]\&. If only one value is given, the same mutation rate Pn is used for SNPs and indels\&. .RE .PP \fB\-p, \-\-pval\-threshold\fR \fIfloat\fR .RS 4 with \fB\-c\fR, accept variant if P(ref|D) < \fIfloat\fR\&. .RE .PP \fB\-P, \-\-prior\fR \fIfloat\fR .RS 4 expected substitution rate, or 0 to disable the prior\&. .RE .PP \fB\-t, \-\-targets\fR \fIfile\fR|\fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-X, \-\-chromosome\-X\fR .RS 4 haploid output for male samples (requires PED file with \fB\-s\fR) .RE .PP \fB\-Y, \-\-chromosome\-Y\fR .RS 4 haploid output for males and skips females (requires PED file with \fB\-s\fR) .RE .RE .SS "bcftools concat \fI[OPTIONS]\fR \fIFILE1\fR \fIFILE2\fR [\&...]" .sp Concatenate or combine VCF/BCF files\&. All source files must have the same sample columns appearing in the same order\&. Can be used, for example, to concatenate chromosome VCFs into one VCF, or combine a SNP VCF and an indel VCF into one\&. The input files must be sorted by chr and position\&. The files must be given in the correct order to produce sorted VCF on output unless the \fB\-a, \-\-allow\-overlaps\fR option is specified\&. .PP \fB\-a, \-\-allow\-overlaps\fR .RS 4 First coordinate of the next file can precede last record of the current file\&. .RE .PP \fB\-D, \-\-remove\-duplicates\fR .RS 4 If a record is present in multiple files, output only the first instance\&. Requires \fB\-a, \-\-allow\-overlaps\fR\&. .RE .PP \fB\-f, \-\-file\-list\fR \fIFILE\fR .RS 4 Read the list of files from a file\&. .RE .PP \fB\-l, \-\-ligate\fR .RS 4 Ligate phased VCFs by matching phase at overlapping haplotypes .RE .PP \fB\-o, \-\-output\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-O, \-\-output\-type\fR \fIb\fR|\fIu\fR|\fIz\fR|\fIv\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-q, \-\-min\-PQ\fR \fIINT\fR .RS 4 Break phase set if phasing quality is lower than \fIINT\fR .RE .PP \fB\-r, \-\-regions\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR\&. Requires \fB\-a, \-\-allow\-overlaps\fR\&. .RE .PP \fB\-R, \-\-regions\-file\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR\&. Requires \fB\-a, \-\-allow\-overlaps\fR\&. .RE .SS "bcftools consensus \fI[OPTIONS]\fR \fIFILE\fR" .sp Create consensus sequence by applying VCF variants to a reference fasta file\&. .PP \fB\-f, \-\-fasta\-ref\fR \fIFILE\fR .RS 4 reference sequence in fasta format .RE .PP \fB\-H, \-\-haplotype\fR \fI1\fR|\fI2\fR .RS 4 apply variants for the given haplotype\&. This option requires \fB\-s\fR, unless exactly one sample is present in the VCF .RE .PP \fB\-i, \-\-iupac\-codes\fR .RS 4 output variants in the form of IUPAC ambiguity codes .RE .PP \fB\-m, \-\-mask\fR \fIFILE\fR .RS 4 BED file or TAB file with regions to be replaced with N\&. See discussion of \fB\-\-regions\-file\fR in \fBCommon Options\fR for file format details\&. .RE .PP \fB\-o, \-\-output\fR \fIFILE\fR .RS 4 write output to a file .RE .PP \fB\-s, \-\-sample\fR \fINAME\fR .RS 4 apply variants of the given sample .RE .sp \fBExamples:\fR .sp .if n \{\ .RS 4 .\} .nf # Apply variants present in sample "NA001", output IUPAC codes for hets bcftools consensus \-i \-s NA001 \-f in\&.fa in\&.vcf\&.gz > out\&.fa # Create consensus for one region\&. The fasta header lines are then expected # in the form ">chr:from\-to"\&. samtools faidx ref\&.fa 8:11870\-11890 | bcftools consensus in\&.vcf\&.gz \-o out\&.fa .fi .if n \{\ .RE .\} .SS "bcftools convert \fI[OPTIONS]\fR \fIFILE\fR" .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBVCF input options:\fR .RS 4 .PP \fB\-e, \-\-exclude\fR \fIEXPRESSION\fR .RS 4 exclude sites for which \fIEXPRESSION\fR is true\&. For valid expressions see \fBEXPRESSIONS\fR\&. .RE .PP \fB\-i, \-\-include\fR \fIEXPRESSION\fR .RS 4 include only sites for which \fIEXPRESSION\fR is true\&. For valid expressions see \fBEXPRESSIONS\fR\&. .RE .PP \fB\-r, \-\-regions\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-R, \-\-regions\-file\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-s, \-\-samples\fR \fILIST\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-S, \-\-samples\-file\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-t, \-\-targets\fR \fILIST\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-T, \-\-targets\-file\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBVCF output options:\fR .RS 4 .PP \fB\-o, \-\-output\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-O, \-\-output\-type\fR \fIb\fR|\fIu\fR|\fIz\fR|\fIv\fR .RS 4 see \fBCommon Options\fR .RE .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBGEN/SAMPLE conversion:\fR .RS 4 .PP \fB\-G, \-\-gensample2vcf\fR \fIprefix\fR or \fIgen\-file\fR,\fIsample\-file\fR .RS 4 convert IMPUTE2 output to VCF\&. The second column must be of the form "CHROM:POS_REF_ALT" to detect possible strand swaps; IMPUTE2 leaves the first one empty ("\-\-") when sites from reference panel are filled in\&. See also \fB\-g\fR below\&. .RE .PP \fB\-g, \-\-gensample\fR \fIprefix\fR or \fIgen\-file\fR,\fIsample\-file\fR .RS 4 convert from VCF to gen/sample format used by IMPUTE2 and SHAPEIT\&. The columns of \&.gen file format are ID1,ID2,POS,A,B followed by three genotype probabilities P(AA), P(AB), P(BB) for each sample\&. In order to prevent strand swaps, the program uses IDs of the form "CHROM:POS_REF_ALT"\&. For example: .RE .sp .if n \{\ .RS 4 .\} .nf \&.gen \-\-\-\- 1:111485207_G_A 1:111485207_G_A 111485207 G A 0 1 0 0 1 0 1:111494194_C_T 1:111494194_C_T 111494194 C T 0 1 0 0 0 1 \&.sample \-\-\-\-\-\-\- ID_1 ID_2 missing 0 0 0 sample1 sample1 0 sample2 sample2 0 .fi .if n \{\ .RE .\} .PP \fB\-\-tag\fR \fISTRING\fR .RS 4 tag to take values for \&.gen file: GT,PL,GL,GP .RE .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBgVCF conversion:\fR .RS 4 .PP \fB\-\-gvcf2vcf\fR .RS 4 convert gVCF to VCF, expanding REF blocks into sites\&. Only sites with FILTER set to "PASS" or "\&." will be expanded\&. .RE .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBHAPS/SAMPLE conversion:\fR .RS 4 .PP \fB\-\-hapsample2vcf\fR \fIprefix\fR or \fIhaps\-file\fR,\fIsample\-file\fR .RS 4 convert from haps/sample format to VCF\&. The columns of \&.haps file are similar to \&.gen file above, but there are only two haplotype columns per sample\&. Note that the first column of the haps file is expected to be in the form "CHR:POS_REF_ALT", for example: .RE .sp .if n \{\ .RS 4 .\} .nf \&.haps \-\-\-\- 1:111485207_G_A rsID1 111485207 G A 0 1 0 0 1:111494194_C_T rsID2 111494194 C T 0 1 0 0 .fi .if n \{\ .RE .\} .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBHAPS/LEGEND/SAMPLE conversion:\fR .RS 4 .PP \fB\-H, \-\-haplegendsample2vcf\fR \fIprefix\fR or \fIhaps\-file\fR,\fIlegend\-file\fR,\fIsample\-file\fR .RS 4 convert from haps/legend/sample format used by IMPUTE2 to VCF, see also \fB\-h, \-\-hapslegendsample\fR below\&. .RE .PP \fB\-h, \-\-haplegendsample\fR \fIprefix\fR or \fIhaps\-file\fR,\fIlegend\-file\fR,\fIsample\-file\fR .RS 4 convert from VCF to haps/legend/sample format used by IMPUTE2 and SHAPEIT\&. The columns of \&.legend file ID,POS,REF,ALT\&. In order to prevent strand swaps, the program uses IDs of the form "CHROM:POS_REF_ALT"\&. The \&.sample file is quite basic at the moment with columns for population, group and sex expected to be edited by the user\&. For example: .RE .sp .if n \{\ .RS 4 .\} .nf \&.haps \-\-\-\-\- 0 1 0 0 1 0 0 1 0 0 0 1 \&.legend \-\-\-\-\-\-\- id position a0 a1 1:111485207_G_A 111485207 G A 1:111494194_C_T 111494194 C T \&.sample \-\-\-\-\-\-\- sample population group sex sample1 sample1 sample1 2 sample2 sample2 sample2 2 .fi .if n \{\ .RE .\} .PP \fB\-\-haploid2diploid\fR .RS 4 with \fB\-h\fR option converts haploid genotypes to homozygous diploid genotypes\&. For example, the program will print \fI0 0\fR instead of the default \fI0 \-\fR\&. This is useful for programs which do not handle haploid genotypes correctly\&. .RE .PP \fB\-\-vcf\-ids\fR .RS 4 output VCF IDs instead of "CHROM:POS_REF_ALT" IDs .RE .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBTSV conversion:\fR .RS 4 .PP \fB\-\-tsv2vcf\fR \fIfile\fR .RS 4 convert from TSV (tab\-separated values) format (such as generated by 23andMe) to VCF\&. The input file fields can be tab\- or space\- delimited .RE .PP \fB\-c, \-\-columns\fR \fIlist\fR .RS 4 comma\-separated list of fields in the input file\&. In the current version, the fields CHROM, POS, ID, and AA are expected and can appear in arbitrary order, columns which should be ignored in the input file can be indicated by "\-"\&. The AA field lists alleles on the forward reference strand, for example "CC" or "CT" for diploid genotypes or "C" for haploid genotypes (sex chromosomes)\&. Insertions and deletions are not supported yet, missing data can be indicated with "\-\-"\&. .RE .PP \fB\-f, \-\-fasta\-ref\fR \fIfile\fR .RS 4 reference sequence in fasta format .RE .PP \fB\-s, \-\-samples\fR \fILIST\fR .RS 4 list of sample names\&. See \fBCommon Options\fR .RE .PP \fB\-S, \-\-samples\-file\fR \fIFILE\fR .RS 4 file of sample names\&. See \fBCommon Options\fR .RE .sp \fBExample:\fR .sp .if n \{\ .RS 4 .\} .nf # Convert 23andme results into VCF bcftools convert \-c ID,CHROM,POS,AA \-s SampleName \-f 23andme\-ref\&.fa \-\-tsv2vcf 23andme\&.txt \-Oz \-o out\&.vcf\&.gz .fi .if n \{\ .RE .\} .RE .SS "bcftools filter \fI[OPTIONS]\fR \fIFILE\fR" .sp Apply fixed\-threshold filters\&. .PP \fB\-e, \-\-exclude\fR \fIEXPRESSION\fR .RS 4 exclude sites for which \fIEXPRESSION\fR is true\&. For valid expressions see \fBEXPRESSIONS\fR\&. .RE .PP \fB\-g, \-\-SnpGap\fR \fIINT\fR .RS 4 filter SNPs within \fIINT\fR base pairs of an indel\&. The following example demonstrates the logic of \fB\-\-SnpGap\fR \fI3\fR applied on a deletion and an insertion: .RE .sp .if n \{\ .RS 4 .\} .nf The SNPs at positions 1 and 7 are filtered, positions 0 and 8 are not: 0123456789 ref \&.G\&.GT\&.\&.G\&.\&. del \&.A\&.G\-\&.\&.A\&.\&. Here the positions 1 and 6 are filtered, 0 and 7 are not: 0123\-456789 ref \&.G\&.G\-\&.\&.G\&.\&. ins \&.A\&.GT\&.\&.A\&.\&. .fi .if n \{\ .RE .\} .PP \fB\-G, \-\-IndelGap\fR \fIINT\fR .RS 4 filter clusters of indels separated by \fIINT\fR or fewer base pairs allowing only one to pass\&. The following example demonstrates the logic of \fB\-\-IndelGap\fR \fI2\fR applied on a deletion and an insertion: .RE .sp .if n \{\ .RS 4 .\} .nf The second indel is filtered: 012345678901 ref \&.GT\&.GT\&.\&.GT\&.\&. del \&.G\-\&.G\-\&.\&.G\-\&.\&. And similarly here, the second is filtered: 01 23 456 78 ref \&.A\-\&.A\-\&.\&.A\-\&.\&. ins \&.AT\&.AT\&.\&.AT\&.\&. .fi .if n \{\ .RE .\} .PP \fB\-i, \-\-include\fR \fIEXPRESSION\fR .RS 4 include only sites for which \fIEXPRESSION\fR is true\&. For valid expressions see \fBEXPRESSIONS\fR\&. .RE .PP \fB\-m, \-\-mode\fR [\fI+x\fR] .RS 4 define behaviour at sites with existing FILTER annotations\&. The default mode replaces existing filters of failed sites with a new FILTER string while leaving sites which pass untouched when non\-empty and setting to "PASS" when the FILTER string is absent\&. The "+" mode appends new FILTER strings of failed sites instead of replacing them\&. The "x" mode resets filters of sites which pass to "PASS"\&. Modes "+" and "x" can both be set\&. .RE .PP \fB\-o, \-\-output\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-O, \-\-output\-type\fR \fIb\fR|\fIu\fR|\fIz\fR|\fIv\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-r, \-\-regions\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-R, \-\-regions\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-s, \-\-soft\-filter\fR \fISTRING\fR|\fI+\fR .RS 4 annotate FILTER column with \fISTRING\fR or, with \fI+\fR, a unique filter name generated by the program ("Filter%d")\&. .RE .PP \fB\-S, \-\-set\-GTs\fR \fI\&.\fR|\fI0\fR .RS 4 set genotypes of failed samples to missing value (\fI\&.\fR) or reference allele (\fI0\fR) .RE .PP \fB\-t, \-\-targets\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-T, \-\-targets\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .SS "bcftools gtcheck [\fIOPTIONS\fR] [\-g \fIgenotypes\&.vcf\&.gz\fR] \fIquery\&.vcf\&.gz\fR" .sp Checks sample identity or, without \fB\-g\fR, multi\-sample cross\-check is performed\&. .PP \fB\-a, \-\-all\-sites\fR .RS 4 output for all sites .RE .PP \fB\-g, \-\-genotypes\fR \fIgenotypes\&.vcf\&.gz\fR .RS 4 reference genotypes to compare against .RE .PP \fB\-G, \-\-GTs\-only\fR \fIINT\fR .RS 4 use genotypes (GT) instead of genotype likelihoods (PL)\&. When set to 1, reported discordance is the number of non\-matching GTs, otherwise the number \fIINT\fR is interpreted as phred\-scaled likelihood of unobserved genotypes\&. .RE .PP \fB\-H, \-\-homs\-only\fR .RS 4 consider only genotypes which are homozygous in both \fIgenotypes\fR and \fIquery\fR VCF\&. This may be useful with low coverage data\&. .RE .PP \fB\-p, \-\-plot\fR \fIPREFIX\fR .RS 4 produce plots .RE .PP \fB\-r, \-\-regions\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-R, \-\-regions\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-s, \-\-query\-sample\fR \fISTRING\fR .RS 4 query sample in \fIquery\&.vcf\&.gz\fR\&. By default, the first sample is checked\&. .RE .PP \fB\-S, \-\-target\-sample\fR \fISTRING\fR .RS 4 target sample in the \fB\-g\fR file, used only for plotting, not for analysis .RE .PP \fB\-t, \-\-targets\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-T, \-\-targets\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBOutput files format:\fR .RS 4 .PP CN, Discordance .RS 4 Pairwise discordance for all sample pairs is calculated as .RE .sp .if n \{\ .RS 4 .\} .nf \esum_s { min_G { PL_a(G) + PL_b(G) } }, .fi .if n \{\ .RE .\} .PP .RS 4 where the sum runs over all sites \fIs\fR and \fIG\fR is the the most likely genotype shared by both samples \fIa\fR and \fIb\fR\&. When PL field is not present, a constant value \fI99\fR is used for the unseen genotypes\&. With \fB\-G\fR, the value \fI1\fR can be used instead; the discordance value then gives exactly the number of differing genotypes\&. .RE .PP SM, Average Discordance .RS 4 Average discordance between sample \fIa\fR and all other samples\&. .RE .PP SM, Average Depth .RS 4 Average depth at evaluated sites, or 1 if FORMAT/DP field is not present\&. .RE .PP SM, Average Number of sites .RS 4 The average number of sites used to calculate the discordance\&. In other words, the average number of non\-missing PLs/genotypes seen both samples\&. .RE .RE .SS "bcftools index [\fIOPTIONS\fR] \fI|\fR" .sp Creates index for bgzip compressed VCF/BCF files for random access\&. CSI (coordinate\-sorted index) is created by default\&. The CSI format supports indexing of chromosomes up to length 2^31\&. TBI (tabix index) index files, which support chromosome lengths up to 2^29, can be created by using the \fI\-t/\-\-tbi\fR option or using the \fItabix\fR program packaged with htslib\&. When loading an index file, bcftools will try the CSI first and then the TBI\&. .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBIndexing options:\fR .RS 4 .PP \fB\-c, \-\-csi\fR .RS 4 generate CSI\-format index for VCF/BCF files [default] .RE .PP \fB\-f, \-\-force\fR .RS 4 overwrite index if it already exists .RE .PP \fB\-m, \-\-min\-shift \fR\fB\fIINT\fR\fR .RS 4 set minimal interval size for CSI indices to 2^INT; default: 14 .RE .PP \fB\-t, \-\-tbi\fR .RS 4 generate TBI\-format index for VCF files .RE .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBStats options:\fR .RS 4 .PP \fB\-n, \-\-nrecords\fR .RS 4 print the number of records based on the CSI or TBI index files .RE .PP \fB\-s, \-\-stats\fR .RS 4 Print per contig stats based on the CSI or TBI index files\&. Output format is three tab\-delimited columns listing the contig name, contig length (\fI\&.\fR if unknown) and number of records for the contig\&. Contigs with zero records are not printed\&. .RE .RE .SS "bcftools isec [\fIOPTIONS\fR] \fIA\&.vcf\&.gz\fR \fIB\&.vcf\&.gz\fR [\&...]" .sp Creates intersections, unions and complements of VCF files\&. Depending on the options, the program can output records from one (or more) files which have (or do not have) corresponding records with the same position in the other files\&. .PP \fB\-c, \-\-collapse\fR \fIsnps\fR|\fIindels\fR|\fIboth\fR|\fIall\fR|\fIsome\fR|\fInone\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-C, \-\-complement\fR .RS 4 output positions present only in the first file but missing in the others .RE .PP \fB\-e, \-\-exclude\fR \fI\-\fR|\fIEXPRESSION\fR .RS 4 exclude sites for which \fIEXPRESSION\fR is true\&. If \fB\-e\fR (or \fB\-i\fR) appears only once, the same filtering expression will be applied to all input files\&. Otherwise, \fB\-e\fR or \fB\-i\fR must be given for each input file\&. To indicate that no filtering should be performed on a file, use "\-" in place of \fIEXPRESSION\fR, as shown in the example below\&. For valid expressions see \fBEXPRESSIONS\fR\&. .RE .PP \fB\-f, \-\-apply\-filters\fR \fILIST\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-i, \-\-include\fR \fIEXPRESSION\fR .RS 4 include only sites for which \fIEXPRESSION\fR is true\&. See discussion of \fB\-e, \-\-exclude\fR above\&. .RE .PP \fB\-n, \-\-nfiles\fR [+\-=]\fIINT\fR .RS 4 output positions present in this many (=), this many or more (+), or this many or fewer (\-) files .RE .PP \fB\-o, \-\-output\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR\&. When several files are being output, their names are controlled via \fB\-p\fR instead\&. .RE .PP \fB\-O, \-\-output\-type\fR \fIb\fR|\fIu\fR|\fIz\fR|\fIv\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-p, \-\-prefix\fR \fIDIR\fR .RS 4 if given, subset each of the input files accordingly\&. See also \fB\-w\fR\&. .RE .PP \fB\-r, \-\-regions\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-R, \-\-regions\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-t, \-\-targets\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-T, \-\-targets\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-w, \-\-write\fR \fILIST\fR .RS 4 list of input files to output given as 1\-based indices\&. With \fB\-p\fR and no \fB\-w\fR, all files are written\&. .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBExamples:\fR .RS 4 .sp Create intersection and complements of two sets saving the output in dir/* .sp .if n \{\ .RS 4 .\} .nf bcftools isec \-p dir A\&.vcf\&.gz B\&.vcf\&.gz .fi .if n \{\ .RE .\} .sp Filter sites in A and B (but not in C) and create intersection .sp .if n \{\ .RS 4 .\} .nf bcftools isec \-e\*(AqMAF<0\&.01\*(Aq \-i\*(AqdbSNP=1\*(Aq \-e\- A\&.vcf\&.gz B\&.vcf\&.gz C\&.vcf\&.gz \-p dir .fi .if n \{\ .RE .\} .sp Extract and write records from A shared by both A and B using exact allele match .sp .if n \{\ .RS 4 .\} .nf bcftools isec \-p dir \-n=2 \-w1 A\&.vcf\&.gz B\&.vcf\&.gz .fi .if n \{\ .RE .\} .sp Extract records private to A or B comparing by position only .sp .if n \{\ .RS 4 .\} .nf bcftools isec \-p dir \-n\-1 \-c all A\&.vcf\&.gz B\&.vcf\&.gz .fi .if n \{\ .RE .\} .RE .SS "bcftools merge [\fIOPTIONS\fR] \fIA\&.vcf\&.gz\fR \fIB\&.vcf\&.gz\fR [\&...]" .sp Merge multiple VCF/BCF files from non\-overlapping sample sets to create one multi\-sample file\&. For example, when merging file \fIA\&.vcf\&.gz\fR containing samples \fIS1\fR, \fIS2\fR and \fIS3\fR and file \fIB\&.vcf\&.gz\fR containing samples \fIS3\fR and \fIS4\fR, the output file will contain four samples named \fIS1\fR, \fIS2\fR, \fIS3\fR, \fI2:S3\fR and \fIS4\fR\&. .sp Note that it is responsibility of the user to ensure that the sample names are unique across all files\&. If they are not, the program will exit with an error unless the option \fB\-\-force\-samples\fR is given\&. The sample names can be also given explicitly using the \fB\-\-print\-header\fR and \fB\-\-use\-header\fR options\&. .sp Note that only records from different files can be merged, never from the same file\&. For "vertical" merge take a look at \fBbcftools norm\fR instead\&. .PP \fB\-\-force\-samples\fR .RS 4 if the merged files contain duplicate samples names, proceed anyway\&. Duplicate sample names will be resolved by prepending index of the file as it appeared on the command line to the conflicting sample name (see \fI2:S3\fR in the above example)\&. .RE .PP \fB\-\-print\-header\fR .RS 4 print only merged header and exit .RE .PP \fB\-\-use\-header\fR \fIFILE\fR .RS 4 use the VCF header in the provided text \fIFILE\fR .RE .PP \fB\-f, \-\-apply\-filters\fR \fILIST\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-i, \-\-info\-rules\fR \fI\-\fR|\fITAG:METHOD\fR[,\&...] .RS 4 Rules for merging INFO fields (scalars or vectors) or \fI\-\fR to disable the default rules\&. \fIMETHOD\fR is one of \fIsum\fR, \fIavg\fR, \fImin\fR, \fImax\fR, \fIjoin\fR\&. .RE .PP \fB\-l, \-\-file\-list\fR \fIFILE\fR .RS 4 read file names from \fIFILE\fR .RE .PP \fB\-m, \-\-merge\fR \fIsnps\fR|\fIindels\fR|\fIboth\fR|\fIall\fR|\fInone\fR|\fIid\fR .RS 4 The option controls what types of multiallelic records can be created: .RE .sp .if n \{\ .RS 4 .\} .nf \-m none \&.\&. no new multiallelics, output multiple records instead \-m snps \&.\&. allow multiallelic SNP records \-m indels \&.\&. allow multiallelic indel records \-m both \&.\&. both SNP and indel records can be multiallelic \-m all \&.\&. SNP records can be merged with indel records \-m id \&.\&. merge by ID .fi .if n \{\ .RE .\} .PP \fB\-o, \-\-output\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-O, \-\-output\-type\fR \fIb\fR|\fIu\fR|\fIz\fR|\fIv\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-r, \-\-regions\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-R, \-\-regions\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .SS "bcftools norm [\fIOPTIONS\fR] \fIfile\&.vcf\&.gz\fR" .sp Left\-align and normalize indels, check if REF alleles match the reference, split multiallelic sites into multiple rows; recover multiallelics from multiple rows\&. .PP \fB\-D, \-\-remove\-duplicates\fR .RS 4 remove duplicate lines of the same type .RE .PP \fB\-f, \-\-fasta\-ref\fR \fIFILE\fR .RS 4 reference sequence .RE .PP \fB\-m, \-\-multiallelics\fR â†|+>[\fIsnps\fR|\fIindels\fR|\fIboth\fR|\fIany\fR] .RS 4 split multiallelic sites into biallelic records (\fI\-\fR) or join biallelic sites into multiallelic records (\fI+\fR)\&. An optional type string can follow which controls variant types which should be split or merged together: If only SNP records should be split or merged, specify \fIsnps\fR; if both SNPs and indels should be merged separately into two records, specify \fIboth\fR; if SNPs and indels should be merged into a single record, specify \fIany\fR\&. .RE .PP \fB\-o, \-\-output\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-O, \-\-output\-type\fR \fIb\fR|\fIu\fR|\fIz\fR|\fIv\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-r, \-\-regions\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-R, \-\-regions\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-s, \-\-strict\-filter\fR .RS 4 when merging (\fI\-m+\fR), merged site is PASS only if all sites being merged PASS .RE .PP \fB\-t, \-\-targets\fR \fILIST\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-T, \-\-targets\-file\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-w, \-\-site\-win\fR \fIINT\fR .RS 4 maximum distance between two records to consider when locally sorting variants which changed position during the realignment .RE .SS "bcftools plugin \fINAME\fR \fI[OPTIONS]\fR \fIFILE\fR \(em \fI[PLUGIN OPTIONS]\fR" .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBVCF input options:\fR .RS 4 .PP \fB\-e, \-\-exclude\fR \fIEXPRESSION\fR .RS 4 exclude sites for which \fIEXPRESSION\fR is true\&. For valid expressions see \fBEXPRESSIONS\fR\&. .RE .PP \fB\-i, \-\-include\fR \fIEXPRESSION\fR .RS 4 include only sites for which \fIEXPRESSION\fR is true\&. For valid expressions see \fBEXPRESSIONS\fR\&. .RE .PP \fB\-r, \-\-regions\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-R, \-\-regions\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-t, \-\-targets\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-T, \-\-targets\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBVCF output options:\fR .RS 4 .PP \fB\-o, \-\-output\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-O, \-\-output\-type\fR \fIb\fR|\fIu\fR|\fIz\fR|\fIv\fR .RS 4 see \fBCommon Options\fR .RE .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBPlugin options:\fR .RS 4 .PP \fB\-h, \-\-help\fR .RS 4 list plugin\(cqs options .RE .PP \fB\-l, \-\-list\-plugins\fR .RS 4 List all available plugins\&. If not installed systemwide, set the environment variable LD_LIBRARY_PATH (linux) or DYLD_LIBRARY_PATH (Mac OS X) to include directory where \fBlibhts\&.so\fR is located\&. The BCFTOOLS_PLUGINS environment variable tells the program which directories to search\&. .RE .PP \fB\-v, \-\-verbose\fR .RS 4 print debugging information to debug plugin failure .RE .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBList of plugins coming with the distribution:\fR .RS 4 .PP \fBcounts\fR .RS 4 a minimal plugin which counts number of SNPs, Indels, and total number of sites\&. .RE .PP \fBdosage\fR .RS 4 print genotype dosage\&. By default the plugin searches for PL, GL and GT, in that order\&. .RE .PP \fBfill\-AN\-AC\fR .RS 4 fill INFO fields AN and AC\&. .RE .PP \fBfix\-ploidy\fR .RS 4 sets correct ploidy .RE .PP \fBframeshifts\fR .RS 4 annotate frameshift indels .RE .PP \fBmissing2ref\fR .RS 4 sets missing genotypes ("\&./\&.") to ref allele ("0/0" or "0|0") .RE .PP \fBtag2tag\fR .RS 4 Convert between similar tags, such as GL and GP\&. .RE .PP \fBvcf2sex\fR .RS 4 determine sample sex by checking genotypes in haploid regions .RE .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBExamples:\fR .RS 4 .sp .if n \{\ .RS 4 .\} .nf # List options common to all plugins bcftools plugin # List available plugins bcftools plugin \-l # One can run plugins in several ways bcftools plugin counts in\&.vcf bcftools +counts in\&.vcf cat in\&.vcf | bcftools +counts # Print usage information of plugin "dosage" bcftools +dosage \-h # Replace missing genotypes with 0/0 bcftools +missing2ref in\&.vcf # Replace missing genotypes with 0|0 bcftools +missing2ref in\&.vcf \-\- \-p .fi .if n \{\ .RE .\} .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBPlugins troubleshooting:\fR .RS 4 .sp Things to check if your plugin does not show up in the \fBbcftools plugin \-l\fR output: .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} Run with the \fB\-v\fR option for verbose output: \fBbcftools plugin \-lv\fR .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} Does the environment variable BCFTOOLS_PLUGINS include the correct path? .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} Are all shared libraries, namely libhts\&.so, accessible? Verify with .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} on Mac OS X: \fBotool \-L your/plugin\&.so\fR and set DYLD_LIBRARY_PATH if they are not .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} on Linux: \fBldd your/plugin\&.so\fR and set LD_LIBRARY_PATH if they are not .RE .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} If not installed systemwide, set the environment variable LD_LIBRARY_PATH (linux) or DYLD_LIBRARY_PATH (mac) to include directory where \fBlibhts\&.so\fR is located\&. .RE .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBPlugins API:\fR .RS 4 .sp .if n \{\ .RS 4 .\} .nf // Short description used by \*(Aqbcftools plugin \-l\*(Aq const char *about(void); // Longer description used by \*(Aqbcftools +name \-h\*(Aq const char *usage(void); // Called once at startup, allows to initialize local variables\&. // Return 1 to suppress normal VCF/BCF header output, \-1 on critical // errors, 0 otherwise\&. int init(int argc, char **argv, bcf_hdr_t *in_hdr, bcf_hdr_t *out_hdr); // Called for each VCF record, return NULL to suppress the output bcf1_t *process(bcf1_t *rec); // Called after all lines have been processed to clean up void destroy(void); .fi .if n \{\ .RE .\} .RE .SS "bcftools query [\fIOPTIONS\fR] \fIfile\&.vcf\&.gz\fR [\fIfile\&.vcf\&.gz\fR [\&...]]" .sp Extracts fields from VCF or BCF files and outputs them in user\-defined format\&. .PP \fB\-c, \-\-collapse\fR \fIsnps\fR|\fIindels\fR|\fIboth\fR|\fIall\fR|\fIsome\fR|\fInone\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-e, \-\-exclude\fR \fIEXPRESSION\fR .RS 4 exclude sites for which \fIEXPRESSION\fR is true\&. For valid expressions see \fBEXPRESSIONS\fR\&. .RE .PP \fB\-f, \-\-format\fR \fIFORMAT\fR .RS 4 learn by example, see below .RE .PP \fB\-H, \-\-print\-header\fR .RS 4 print header .RE .PP \fB\-i, \-\-include\fR \fIEXPRESSION\fR .RS 4 include only sites for which \fIEXPRESSION\fR is true\&. For valid expressions see \fBEXPRESSIONS\fR\&. .RE .PP \fB\-l, \-\-list\-samples\fR .RS 4 list sample names and exit .RE .PP \fB\-o, \-\-output\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-r, \-\-regions\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-R, \-\-regions\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-s, \-\-samples\fR \fILIST\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-S, \-\-samples\-file\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-t, \-\-targets\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-T, \-\-targets\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-v, \-\-vcf\-list\fR \fIFILE\fR .RS 4 process multiple VCFs listed in the file .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBFormat:\fR .RS 4 .sp .if n \{\ .RS 4 .\} .nf %CHROM The CHROM column (similarly also other columns: POS, ID, REF, ALT, QUAL, FILTER) %INFO/TAG Any tag in the INFO column %TYPE Variant type (REF, SNP, MNP, INDEL, OTHER) %MASK Indicates presence of the site in other files (with multiple files) %TAG{INT} Curly brackets to subscript vectors (0\-based) %FIRST_ALT Alias for %ALT{0} [] The brackets loop over all samples %GT Genotype (e\&.g\&. 0/1) %TGT Translated genotype (e\&.g\&. C/A) %IUPACGT Genotype translated to IUPAC ambiguity codes (e\&.g\&. M instead of C/A) %LINE Prints the whole line %SAMPLE Sample name .fi .if n \{\ .RE .\} .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBExamples:\fR .RS 4 .sp .if n \{\ .RS 4 .\} .nf bcftools query \-f \*(Aq%CHROM %POS %REF %ALT{0}\en\*(Aq file\&.vcf\&.gz bcftools query \-f \*(Aq%CHROM\et%POS\et%REF\et%ALT[\et%SAMPLE=%GT]\en\*(Aq file\&.vcf\&.gz .fi .if n \{\ .RE .\} .RE .SS "bcftools reheader [\fIOPTIONS\fR] \fIfile\&.vcf\&.gz\fR" .sp Modify header of VCF/BCF files, change sample names\&. .PP \fB\-h, \-\-header\fR \fIFILE\fR .RS 4 new VCF header .RE .PP \fB\-o, \-\-output\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-s, \-\-samples\fR \fIFILE\fR .RS 4 new sample names, one name per line, in the same order as they appear in the VCF file\&. Alternatively, only samples which need to be renamed can be listed as "old_name new_name\en" pairs separated by whitespaces, each on separate line\&. .RE .SS "bcftools roh [\fIOPTIONS\fR] \fIfile\&.vcf\&.gz\fR" .sp A program for detecting runs of homo/autozygosity\&. Only bi\-allelic sites are considered\&. .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBThe HMM model:\fR .RS 4 .sp .if n \{\ .RS 4 .\} .nf Notation: D = Data, AZ = autozygosity, HW = Hardy\-Weinberg (non\-autozygosity), f = non\-ref allele frequency Emission probabilities: oAZ = P_i(D|AZ) = (1\-f)*P(D|RR) + f*P(D|AA) oHW = P_i(D|HW) = (1\-f)^2 * P(D|RR) + f^2 * P(D|AA) + 2*f*(1\-f)*P(D|RA) Transition probabilities: tAZ = P(AZ|HW) \&.\&. from HW to AZ, the \-a parameter tHW = P(HW|AZ) \&.\&. from AZ to HW, the \-H parameter P(AZ|AZ) = 1 \- P(HW|AZ) = 1 \- tHW P(HW|HW) = 1 \- P(AZ|HW) = 1 \- tAZ ci = P_i(C) \&.\&. probability of cross\-over at site i, from genetic map AZi = P_i(AZ) \&.\&. probability of site i being AZ/non\-AZ, scaled so that AZi+HWi = 1 HWi = P_i(HW) P_{i+1}(AZ) = oAZ * max[(1\-tHW) * (1\-ci) * AZ{i\-1} , tAZ * ci * (1\-AZ{i\-1})] P_{i+1}(HW) = oHW * max[(1\-tAZ) * (1\-ci) * (1\-AZ{i\-1}) , tHW * ci * AZ{i\-1}] .fi .if n \{\ .RE .\} .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBGeneral Options:\fR .RS 4 .PP \fB\-\-AF\-tag\fR \fITAG\fR .RS 4 use the specified INFO tag \fITAG\fR as an allele frequency estimate instead of the defaul AC and AN tags\&. Sites which do not have \fITAG\fR will be skipped\&. .RE .PP \fB\-\-AF\-file\fR \fIFILE\fR .RS 4 Read allele frequencies from a tab\-delimited file containing the columns: CHROM\etPOS\etREF,ALT\etAF\&. The file can be compressed with \fBbgzip\fR and indexed with tabix \-s1 \-b2 \-e2\&. Sites which are not present in the \fIFILE\fR or have different reference or alternate allele will be skipped\&. Note that such a file can be easily created from a VCF using: .RE .sp .if n \{\ .RS 4 .\} .nf bcftools query \-f\*(Aq%CHROM\et%POS\et%REF,%ALT\et%INFO/TAG\en\*(Aq file\&.vcf | bgzip \-c > freqs\&.tab\&.gz .fi .if n \{\ .RE .\} .PP \fB\-e, \-\-estimate\-AF\fR \fIFILE\fR .RS 4 recalculate INFO/AC and INFO/AN on the fly, using either all samples ("\-") or samples listed in \fIFILE\fR\&. By default, allele frequency is estimated from AC and AN counts which are already present in the INFO field\&. .RE .PP \fB\-G, \-\-GTs\-only\fR \fIFLOAT\fR .RS 4 use genotypes (FORMAT/GT fields) ignoring genotype likelihoods (FORMAT/PL), setting PL of unseen genotypes to \fIFLOAT\fR\&. Safe value to use is 30 to account for GT errors\&. .RE .PP \fB\-I, \-\-skip\-indels\fR .RS 4 skip indels as their genotypes are usually enriched for errors .RE .PP \fB\-m, \-\-genetic\-map\fR \fIFILE\fR .RS 4 genetic map in the format required also by IMPUTE2\&. Only the first and third column are used (position and Genetic_Map(cM))\&. The \fIFILE\fR can chromosome name\&. .RE .PP \fB\-r, \-\-regions\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-R, \-\-regions\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-s, \-\-sample\fR \fIname\fR .RS 4 the name of sample to analyze .RE .PP \fB\-t, \-\-targets\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-T, \-\-targets\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBHMM Options:\fR .RS 4 .PP \fB\-a, \-\-hw\-to\-az\fR \fIFLOAT\fR .RS 4 P(AZ|HW) transition probability from AZ (autozygous) to HW (Hardy\-Weinberg) state .RE .PP \fB\-H, \-\-az\-to\-hw\fR \fIFLOAT\fR .RS 4 P(HW|AZ) transition probability from HW to AZ state .RE .PP \fB\-V, \-\-viterbi\-training\fR .RS 4 perform Viterbi training to estimate transition probabilities .RE .RE .SS "bcftools stats [\fIOPTIONS\fR] \fIA\&.vcf\&.gz\fR [\fIB\&.vcf\&.gz\fR]" .sp Parses VCF or BCF and produces text file stats which is suitable for machine processing and can be plotted using \fBplot\-vcfstats\fR\&. When two files are given, the program generates separate stats for intersection and the complements\&. By default only sites are compared, \fB\-s\fR/\fB\-S\fR must given to include also sample columns\&. .PP \fB\-1, \-\-1st\-allele\-only\fR .RS 4 consider only 1st allele at multiallelic sites .RE .PP \fB\-c, \-\-collapse\fR \fIsnps\fR|\fIindels\fR|\fIboth\fR|\fIall\fR|\fIsome\fR|\fInone\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-d, \-\-depth\fR \fIINT\fR,\fIINT\fR,\fIINT\fR .RS 4 ranges of depth distribution: min, max, and size of the bin .RE .PP \fB\-\-debug\fR .RS 4 produce verbose per\-site and per\-sample output .RE .PP \fB\-e, \-\-exons\fR \fIfile\&.gz\fR .RS 4 tab\-delimited file with exons for indel frameshifts statistics\&. The columns of the file are CHR, FROM, TO, with 1\-based, inclusive, positions\&. The file is BGZF\-compressed and indexed with tabix .RE .sp .if n \{\ .RS 4 .\} .nf tabix \-s1 \-b2 \-e3 file\&.gz .fi .if n \{\ .RE .\} .PP \fB\-f, \-\-apply\-filters\fR \fILIST\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-F, \-\-fasta\-ref\fR \fIref\&.fa\fR .RS 4 faidx indexed reference sequence file to determine INDEL context .RE .PP \fB\-i, \-\-split\-by\-ID\fR .RS 4 collect stats separately for sites which have the ID column set ("known sites") or which do not have the ID column set ("novel sites")\&. .RE .PP \fB\-r, \-\-regions\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-R, \-\-regions\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-s, \-\-samples\fR \fILIST\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-S, \-\-samples\-file\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-t, \-\-targets\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-T, \-\-targets\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .SS "bcftools view [\fIOPTIONS\fR] \fIfile\&.vcf\&.gz\fR [\fIREGION\fR [\&...]]" .sp View, subset and filter VCF or BCF files by position and filtering expression\&. Convert between VCF and BCF\&. Former \fBbcftools subset\fR\&. .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBOutput options\fR .RS 4 .PP \fB\-G, \-\-drop\-genotypes\fR .RS 4 drop individual genotype information (after subsetting if \fB\-s\fR option is set) .RE .PP \fB\-h, \-\-header\-only\fR .RS 4 output the VCF header only .RE .PP \fB\-H, \-\-no\-header\fR .RS 4 suppress the header in VCF output .RE .PP \fB\-l, \-\-compression\-level\fR [\fI0\-9\fR] .RS 4 compression level\&. 0 stands for uncompressed, 1 for best speed and 9 for best compression\&. .RE .PP \fB\-O, \-\-output\-type\fR \fIb\fR|\fIu\fR|\fIz\fR|\fIv\fR .RS 4 see \fBCommon Options\fR .RE .sp \fB\-o, \-\-output\-file\fR \fIFILE\fR: output file name\&. If not present, the default is to print to standard output (stdout)\&. .PP \fB\-r, \-\-regions\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-R, \-\-regions\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-t, \-\-targets\fR \fIchr\fR|\fIchr:pos\fR|\fIchr:from\-to\fR|\fIchr:from\-\fR[,\&...] .RS 4 see \fBCommon Options\fR .RE .PP \fB\-T, \-\-targets\-file\fR \fIfile\fR .RS 4 see \fBCommon Options\fR .RE .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBSubset options:\fR .RS 4 .PP \fB\-a, \-\-trim\-alt\-alleles\fR .RS 4 trim alternate alleles not seen in subset\&. Type A, G and R INFO and FORMAT fields will also be trimmed .RE .PP \fB\-I, \-\-no\-update\fR .RS 4 do not (re)calculate INFO fields for the subset (currently INFO/AC and INFO/AN) .RE .PP \fB\-s, \-\-samples\fR \fILIST\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-S, \-\-samples\-file\fR \fIFILE\fR .RS 4 see \fBCommon Options\fR .RE .RE .sp .it 1 an-trap .nr an-no-space-flag 1 .nr an-break-flag 1 .br .ps +1 \fBFilter options:\fR .RS 4 .PP \fB\-c, \-\-min\-ac\fR \fIINT\fR[\fI:nref\fR|\fI:alt1\fR|\fI:minor\fR|\fI:major\fR|:\*(Aqnonmajor\*(Aq] .RS 4 minimum allele count (INFO/AC) of sites to be printed\&. Specifying the type of allele is optional and can be set to non\-reference (\fInref\fR, the default), 1st alternate (\fIalt1\fR), the least frequent (\fIminor\fR), the most frequent (\fImajor\fR) or sum of all but the most frequent (\fInonmajor\fR) alleles\&. .RE .PP \fB\-C, \-\-max\-ac\fR \fIINT\fR[\fI:nref\fR|\fI:alt1\fR|\fI:minor\fR|:\*(Aqmajor\*(Aq|:\*(Aqnonmajor\*(Aq] .RS 4 maximum allele count (INFO/AC) of sites to be printed\&. Specifying the type of allele is optional and can be set to non\-reference (\fInref\fR, the default), 1st alternate (\fIalt1\fR), the least frequent (\fIminor\fR), the most frequent (\fImajor\fR) or sum of all but the most frequent (\fInonmajor\fR) alleles\&. .RE .PP \fB\-e, \-\-exclude\fR \fIEXPRESSION\fR .RS 4 exclude sites for which \fIEXPRESSION\fR is true\&. For valid expressions see \fBEXPRESSIONS\fR\&. .RE .PP \fB\-f, \-\-apply\-filters\fR \fILIST\fR .RS 4 see \fBCommon Options\fR .RE .PP \fB\-g, \-\-genotype\fR [^][\fIhom\fR|\fIhet\fR|\fImiss\fR] .RS 4 include only sites with one or more homozygous (\fIhom\fR), heterozygous (\fIhet\fR) or missing (\fImiss\fR) genotypes\&. When prefixed with \fI^\fR, the logic is reversed; thus \fI^het\fR excludes sites with heterozygous genotypes\&. .RE .PP \fB\-i, \-\-include\fR \fIEXPRESSION\fR .RS 4 include sites for which \fIEXPRESSION\fR is true\&. For valid expressions see \fBEXPRESSIONS\fR\&. .RE .PP \fB\-k, \-\-known\fR .RS 4 print known sites only (ID column is not "\&.") .RE .PP \fB\-m, \-\-min\-alleles\fR \fIINT\fR .RS 4 print sites with at least \fIINT\fR alleles listed in REF and ALT columns .RE .PP \fB\-M, \-\-max\-alleles\fR \fIINT\fR .RS 4 print sites with at most \fIINT\fR alleles listed in REF and ALT columns\&. Use \fB\-m2 \-M2 \-v snps\fR to only view biallelic SNPs\&. .RE .PP \fB\-n, \-\-novel\fR .RS 4 print novel sites only (ID column is "\&.") .RE .PP \fB\-p, \-\-phased\fR .RS 4 print sites where all samples are phased\&. Haploid genotypes are considered phased\&. Missing genotypes considered unphased unless the phased bit is set\&. .RE .PP \fB\-P, \-\-exclude\-phased\fR .RS 4 exclude sites where all samples are phased .RE .PP \fB\-q, \-\-min\-af\fR \fIFLOAT\fR[\fI:nref\fR|\fI:alt1\fR|\fI:minor\fR|:\*(Aqmajor\*(Aq|:\*(Aqnonmajor\*(Aq] .RS 4 minimum allele frequency (INFO/AC / INFO/AN) of sites to be printed\&. Specifying the type of allele is optional and can be set to non\-reference (\fInref\fR, the default), 1st alternate (\fIalt1\fR), the least frequent (\fIminor\fR), the most frequent (\fImajor\fR) or sum of all but the most frequent (\fInonmajor\fR) alleles\&. .RE .PP \fB\-Q, \-\-max\-af\fR \fIFLOAT\fR[\fI:nref\fR|\fI:alt1\fR|\fI:minor\fR|:\*(Aqmajor\*(Aq|:\*(Aqnonmajor\*(Aq] .RS 4 maximum allele frequency (INFO/AC / INFO/AN) of sites to be printed\&. Specifying the type of allele is optional and can be set to non\-reference (\fInref\fR, the default), 1st alternate (\fIalt1\fR), the least frequent (\fIminor\fR), the most frequent (\fImajor\fR) or sum of all but the most frequent (\fInonmajor\fR) alleles\&. .RE .PP \fB\-u, \-\-uncalled\fR .RS 4 print sites without a called genotype .RE .PP \fB\-U, \-\-exclude\-uncalled\fR .RS 4 exclude sites without a called genotype .RE .PP \fB\-v, \-\-types\fR \fIsnps\fR|\fIindels\fR|\fImnps\fR|\fIother\fR .RS 4 comma\-separated list of variant types to select .RE .PP \fB\-V, \-\-exclude\-types\fR \fIsnps\fR|\fIindels\fR|\fImnps\fR|\fIother\fR .RS 4 comma\-separated list of variant types to exclude .RE .PP \fB\-x, \-\-private\fR .RS 4 print sites where only the subset samples carry an non\-reference allele .RE .PP \fB\-X, \-\-exclude\-private\fR .RS 4 exclude sites where only the subset samples carry an non\-reference allele .RE .RE .SH "EXPRESSIONS" .sp These filtering expressions are accepted by \fBannotate\fR, \fBfilter\fR, \fBquery\fR and \fBview\fR commands\&. .PP \fBValid expressions may contain:\fR .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} numerical constants, string constants, file names .sp .if n \{\ .RS 4 .\} .nf 1, 1\&.0, 1e\-4 "String" @file_name .fi .if n \{\ .RE .\} .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} arithmetic operators .sp .if n \{\ .RS 4 .\} .nf +,*,\-,/ .fi .if n \{\ .RE .\} .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} comparison operators .sp .if n \{\ .RS 4 .\} .nf == (same as =), >, >=, <=, <, != .fi .if n \{\ .RE .\} .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} regex operators "~" and its negation "!~" .sp .if n \{\ .RS 4 .\} .nf INFO/HAYSTACK ~ "needle" .fi .if n \{\ .RE .\} .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} parentheses .sp .if n \{\ .RS 4 .\} .nf (, ) .fi .if n \{\ .RE .\} .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} logical operators .sp .if n \{\ .RS 4 .\} .nf && (same as &), ||, | .fi .if n \{\ .RE .\} .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} INFO tags, FORMAT tags, column names .sp .if n \{\ .RS 4 .\} .nf INFO/DP or DP FORMAT/DV, FMT/DV, or DV FILTER, QUAL, ID, REF, ALT[0] .fi .if n \{\ .RE .\} .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} 1 (or 0) to test the presence (or absence) of a flag .sp .if n \{\ .RS 4 .\} .nf FlagA=1 && FlagB=0 .fi .if n \{\ .RE .\} .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} TYPE for variant type in REF,ALT columns (indel,snp,mnp,ref,other) .sp .if n \{\ .RS 4 .\} .nf TYPE="indel" | TYPE="snp" .fi .if n \{\ .RE .\} .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} array subscripts, "*" for any field .sp .if n \{\ .RS 4 .\} .nf (DP4[0]+DP4[1])/(DP4[2]+DP4[3]) > 0\&.3 DP4[*] == 0 CSQ[*] ~ "missense_variant\&.*deleterious" .fi .if n \{\ .RE .\} .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} function on FORMAT tags (over samples) and INFO tags (over vector fields) .sp .if n \{\ .RS 4 .\} .nf MAX, MIN, AVG, SUM, STRLEN, ABS .fi .if n \{\ .RE .\} .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} variables calculated on the fly if not present: number of alternate alleles; number of samples; count of alternate alleles; minor allele count (similar to AC but is always smaller than 0\&.5); frequency of alternate alleles (AF=AC/AN); frequency of minor alleles (MAF=MAC/AN); number of alleles in called genotypes .sp .if n \{\ .RS 4 .\} .nf N_ALT, N_SAMPLES, AC, MAC, AF, MAF, AN .fi .if n \{\ .RE .\} .RE .PP \fBNotes:\fR .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} String comparisons and regular expressions are case\-insensitive .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} If the subscript "*" is used in regular expression search, the whole field is treated as one string\&. For example, the regex STR[*]~"B,C" will be true for the string vector INFO/STR=AB,CD\&. .RE .sp .RS 4 .ie n \{\ \h'-04'\(bu\h'+03'\c .\} .el \{\ .sp -1 .IP \(bu 2.3 .\} Variables and function names are case\-insensitive, but not tag names\&. For example, "qual" can be used instead of "QUAL", "strlen()" instead of "STRLEN()" , but not "dp" instead of "DP"\&. .RE .sp \fBExamples:\fR .sp .if n \{\ .RS 4 .\} .nf MIN(DV)>5 .fi .if n \{\ .RE .\} .sp .if n \{\ .RS 4 .\} .nf MIN(DV/DP)>0\&.3 .fi .if n \{\ .RE .\} .sp .if n \{\ .RS 4 .\} .nf MIN(DP)>10 & MIN(DV)>3 .fi .if n \{\ .RE .\} .sp .if n \{\ .RS 4 .\} .nf QUAL>10 | FMT/GQ>10 \&.\&. selects only GQ>10 samples .fi .if n \{\ .RE .\} .sp .if n \{\ .RS 4 .\} .nf QUAL>10 || FMT/GQ>10 \&.\&. selects all samples at QUAL>10 sites .fi .if n \{\ .RE .\} .sp .if n \{\ .RS 4 .\} .nf TYPE="snp" && QUAL>=10 && (DP4[2]+DP4[3] > 2) .fi .if n \{\ .RE .\} .sp .if n \{\ .RS 4 .\} .nf MIN(DP)>35 && AVG(GQ)>50 .fi .if n \{\ .RE .\} .sp .if n \{\ .RS 4 .\} .nf ID=@file \&.\&. selects lines with ID present in the file .fi .if n \{\ .RE .\} .sp .if n \{\ .RS 4 .\} .nf ID!=@~/file \&.\&. skip lines with ID present in the ~/file .fi .if n \{\ .RE .\} .sp .if n \{\ .RS 4 .\} .nf MAF[0]<0\&.05 \&.\&. select rare variants at 5% cutoff .fi .if n \{\ .RE .\} .sp \fBShell expansion:\fR .sp Note that expressions must often be quoted because some characters have special meaning in the shell\&. An example of expression enclosed in single quotes which cause that the whole expression is passed to the program as intended: .sp .if n \{\ .RS 4 .\} .nf bcftools view \-i \*(Aq%ID!="\&." & MAF[0]<0\&.01\*(Aq .fi .if n \{\ .RE .\} .sp Please refer to the documentation of your shell for details\&. .SH "SCRIPTS AND OPTIONS" .SS "plot\-vcfstats [\fIOPTIONS\fR] \fIfile\&.vchk\fR [\&...]" .sp Script for processing output of \fBbcftools stats\fR\&. It can merge results from multiple outputs (useful when running the stats for each chromosome separately), plots graphs and creates a PDF presentation\&. .PP \fB\-m, \-\-merge\fR .RS 4 Merge vcfstats files to STDOUT, skip plotting\&. .RE .PP \fB\-p, \-\-prefix\fR \fIPATH\fR .RS 4 The output files prefix, add a slash to create new directory\&. .RE .PP \fB\-P, \-\-no\-PDF\fR .RS 4 Skip the PDF creation step\&. .RE .PP \fB\-r, \-\-rasterize\fR .RS 4 Rasterize PDF images for faster rendering\&. .RE .PP \fB\-s, \-\-sample\-names\fR .RS 4 Use sample names for xticks rather than numeric IDs\&. .RE .PP \fB\-t, \-\-title\fR \fISTRING\fR .RS 4 Identify files by these titles in plots\&. The option can be given multiple times, for each ID in the \fBbcftools stats\fR output\&. If not present, the script will use abbreviated source file names for the titles\&. .RE .PP \fB\-T, \-\-main\-title\fR \fISTRING\fR .RS 4 Main title for the PDF\&. .RE .SH "PERFORMANCE" .sp HTSlib was designed with BCF format in mind\&. When parsing VCF files, all records are internally converted into BCF representation\&. Simple operations, like removing a single column from a VCF file, can be therefore done much faster with standard UNIX commands, such as \fBawk\fR or \fBcut\fR\&. Therefore it is recommended to use BCF as input/output format whenever possible to avoid large overhead of the VCF → BCF → VCF conversion\&. .SH "BUGS" .sp Please report any bugs you encounter on the github website: http://github\&.com/samtools/bcftools .SH "AUTHORS" .sp Heng Li from the Sanger Institute wrote the original C version of htslib, samtools and bcftools\&. Bob Handsaker from the Broad Institute implemented the BGZF library\&. Petr Danecek, Shane McCarthy and John Marshall are maintaining and further developing bcftools\&. Many other people contributed to the program and to the file format specifications, both directly and indirectly by providing patches, testing and reporting bugs\&. We thank them all\&. .SH "RESOURCES" .sp BCFtools GitHub website: http://github\&.com/samtools/bcftools .sp Samtools GitHub website: http://github\&.com/samtools/samtools .sp HTSlib GitHub website: http://github\&.com/samtools/htslib .sp File format specifications: http://samtools\&.github\&.io/hts\-specs .sp BCFtools documentation: http://samtools\&.github\&.io/bcftools .sp BCFtools wiki page: https://github\&.com/samtools/bcftools/wiki .SH "COPYING" .sp The MIT/Expat License or GPL License, see the LICENSE document for details\&. Copyright (c) Genome Research Ltd\&. bcftools-1.2/doc/bcftools.html000066400000000000000000004042711246371514100164510ustar00rootroot00000000000000 bcftools

Name

bcftools — utilities for variant calling and manipulating VCFs and BCFs.

Synopsis

bcftools [COMMAND] [OPTIONS]

DESCRIPTION

BCFtools is a set of utilities that manipulate variant calls in the Variant Call Format (VCF) and its binary counterpart BCF. All commands work transparently with both VCFs and BCFs, both uncompressed and BGZF-compressed.

Most commands accept VCF, bgzipped VCF and BCF with filetype detected automatically even when streaming from a pipe. Indexed VCF and BCF will work in all situations. Un-indexed VCF and BCF and streams will work in most, but not all situations.

BCFtools is designed to work on a stream. It regards an input file "-" as the standard input (stdin) and outputs to the standard output (stdout). Several commands can thus be combined with Unix pipes.

VERSION

This manual page was last updated 2015-01-21 15:01 GMT and refers to bcftools git version 1.1-140-g9b0e7cc+.

BCF1

The BCF1 format output by versions of samtools <= 0.1.19 is not compatible with this version of bcftools. To read BCF1 files one can use the view command from old versions of bcftools packaged with samtools versions <= 0.1.19 to convert to VCF, which can then be read by this version of bcftools.

    samtools-0.1.19/bcftools/bcftools view file.bcf1 | bcftools view

VARIANT CALLING

See bcftools call for variant calling from the output of the samtools mpileup command. In versions of samtools <= 0.1.19 calling was done with bcftools view. Users are now required to choose between the old samtools calling model (-c/--consensus-caller) and the new multiallelic calling model (-m/--multiallelic-caller). The multiallelic calling model is recommended for most tasks.

LIST OF COMMANDS

For a full list of available commands, run bcftools without arguments. For a full list of available options, run bcftools COMMAND without arguments.

  • annotate .. edit VCF files, add or remove annotations
  • call .. SNP/indel calling (former "view")
  • concat .. concatenate VCF/BCF files from the same set of samples
  • consensus .. create consensus sequence by applying VCF variants
  • convert .. convert VCF/BCF to other formats and back
  • filter .. filter VCF/BCF files using fixed thresholds
  • gtcheck .. check sample concordance, detect sample swaps and contamination
  • index .. index VCF/BCF
  • isec .. intersections of VCF/BCF files
  • merge .. merge VCF/BCF files files from non-overlapping sample sets
  • norm .. normalize indels
  • plugin .. run user-defined plugin
  • query .. transform VCF/BCF into user-defined formats
  • reheader .. modify VCF/BCF header, change sample names
  • roh .. identify runs of homo/auto-zygosity
  • stats .. produce VCF/BCF stats (former vcfcheck)
  • view .. subset, filter and convert VCF and BCF files

LIST OF SCRIPTS

Some helper scripts are bundled with the bcftools code.

COMMANDS AND OPTIONS

Common Options

The following options are common to many bcftools commands. See usage for specific commands to see if they apply.

FILE
Files can be both VCF or BCF, uncompressed or BGZF-compressed. The file "-" is interpreted as standard input. Some tools may require tabix- or CSI-indexed files.
-c, --collapse snps|indels|both|all|some|none|id

Controls how to treat records with duplicate positions and defines compatible records across multiple input files. Here by "compatible" we mean records which should be considered as identical by the tools. For example, when performing line intersections, the desire may be to consider as identical all sites with matching positions (bcftools isec -c all), or only sites with matching variant type (bcftools isec -c snps  -c indels), or only sites with all alleles identical (bcftools isec -c none).

none
only records with identical REF and ALT alleles are compatible
some
only records where some subset of ALT alleles match are compatible
all
all records are compatible, regardless of whether the ALT alleles match or not. In the case of records with the same position, only the first will be considered and appear on output.
snps
any SNP records are compatible, regardless of whether the ALT alleles match or not. For duplicate positions, only the first SNP record will be considered and appear on output.
indels
all indel records are compatible, regardless of whether the REF and ALT alleles match or not. For duplicate positions, only the first indel record will be considered and appear on output.
both
abbreviation of "-c indels  -c snps"
id
only records with identical ID column are compatible. Supported by bcftools merge only.
-f, --apply-filters LIST
Skip sites where FILTER column does not contain any of the strings listed in LIST. For example, to include only sites which have no filters set, use -f .,PASS.
-o, --output FILE
When output consists of a single stream, write it to FILE rather than to standard output, where it is written by default.
-O, --output-type b|u|z|v
Output compressed BCF (b), uncompressed BCF (u), compressed VCF (z), uncompressed VCF (v).
-r, --regions chr|chr:pos|chr:from-to|chr:from-[,…]
Comma-separated list of regions, see also -R, --regions-file. Note that -r cannot be used in combination with -R.
-R, --regions-file FILE
Regions can be specified either on command line or in a VCF, BED, or tab-delimited file (the default). The columns of the tab-delimited file are: CHROM, POS, and, optionally, POS_TO, where positions are 1-based and inclusive. Uncompressed files are stored in memory, while bgzip-compressed and tabix-indexed region files are streamed. Note that sequence names must match exactly, "chr20" is not the same as "20". Also note that chromosome ordering in FILE will be respected, the VCF will be processed in the order in which chromosomes first appear in FILE. However, within chromosomes, the VCF will always be processed in ascending genomic coordinate order no matter what order they appear in FILE. Note that overlapping regions in FILE can result in duplicated out of order positions in the output. This option requires indexed VCF/BCF files. Note that -R cannot be used in combination with -r.
-s, --samples [^]LIST
Comma-separated list of samples to include or exclude if prefixed with "^".
-S, --samples-file FILE
File of sample names to include or exclude if prefixed with "^". One sample per line. The command bcftools call accepts an optional second column indicating ploidy (0, 1 or 2) and can parse also PED files. With bcftools call -C trio, PED file is expected.
-t, --targets [^]chr|chr:pos|chr:from-to|chr:from-[,…]
Similar as -r, --regions, but the next position is accessed by streaming the whole VCF/BCF rather than using the tbi/csi index. Both -r and -t options can be applied simultaneously: -r uses the index to jump to a region and -t discards positions which are not in the targets. Unlike -r, targets can be prefixed with "^" to request logical complement. For example, "^X,Y,MT" indicates that sequences X, Y and MT should be skipped. Yet another difference between the two is that -r checks both start and end positions of indels, whereas -t checks start positions only. Note that -t cannot be used in combination with -T.
-T, --targets-file [^]FILE
Same -t, --targets, but reads regions from a file. Note that -T cannot be used in combination with -t.
With the call -C alleles command, third column of the targets file must be comma-separated list of alleles, starting with the reference allele. Such a file can be easily created from a VCF using: 
    bcftools query -f'%CHROM\t%POS\t%REF,%ALT\n' file.vcf

bcftools annotate [OPTIONS] FILE

This command allows to add or remove annotations.

-a, --annotations file
Bgzip-compressed and tabix-indexed file with annotations. The file can be VCF, BED, or a tab-delimited file with mandatory columns CHROM, POS (or, alternatively, FROM and TO), optional columns REF and ALT, and arbitrary number of annotation columns. BED files are expected to have the ".bed" or ".bed.gz" suffix (case-insensitive), otherwise a tab-delimited file is assumed. Note that in case of tab-delimited file, the coordinates POS, FROM and TO are one-based and inclusive. When REF and ALT are present, only matching VCF records will be annotated. When multiple ALT alleles are present in the annotation file (given as comma-separated list of alleles), at least one must match one of the alleles in the corresponding VCF record. Similarly, at least one alternate allele from a multi-allelic VCF record must be present in the annotation file. Note that flag types, such as "INFO/FLAG", can be annotated by including a field with the value "1" to set the flag, "0" to remove it, or "." to keep existing flags. See also -c, --columns and -h, --header-lines. 
    # Sample annotation file with columns CHROM, POS, STRING_TAG, NUMERIC_TAG
    1  752566  SomeString      5
    1  798959  SomeOtherString 6
    # etc.
-c, --columns list
Comma-separated list of columns or tags to carry over from the annotation file (see also -a, --annotations). If the annotation file is not a VCF/BCF, list describes the columns of the annotation file and must include CHROM, POS (or, alternatively, FROM and TO), and optionally REF and ALT. Unused columns which should be ignored can be indicated by "-". If the annotation file is a VCF/BCF, only the edited columns/tags must be present and their order does not matter. The columns ID, QUAL, FILTER, INFO and FORMAT can be edited, where INFO tags can be written both as "INFO/TAG" or simply "TAG", and FORMAT tags can be written as "FORMAT/TAG" or "FMT/TAG". To carry over all INFO annotations, use "INFO". To add all INFO annotations except "TAG", use "^INFO/TAG". By default, existing values are replaced. To add values without overwriting existing annotations, use "+TAG" instead of "TAG". To replace only existing values without modifying missing annotations, use "-TAG". If the annotation file is not a VCF/BCF, all new annotations must be defined via -h, --header-lines.
-e, --exclude EXPRESSION
exclude sites for which EXPRESSION is true. For valid expressions see EXPRESSIONS.
-h, --header-lines file
Lines to append to the VCF header, see also -c, --columns and -a, --annotations. For example: 
    ##INFO=<ID=NUMERIC_TAG,Number=1,Type=Integer,Description="Example header line">
    ##INFO=<ID=STRING_TAG,Number=1,Type=String,Description="Yet another header line">
-I, --set-id [+]FORMAT
assign ID on the fly. The format is the same as in the query command (see below). By default all existing IDs are replaced. If the format string is preceded by "+", only missing IDs will be set. For example, one can use 
    bcftools annotate --set-id +'%CHROM\_%POS\_%REF\_%FIRST_ALT' file.vcf
-i, --include EXPRESSION
include only sites for which EXPRESSION is true. For valid expressions see EXPRESSIONS.
-o, --output FILE
see Common Options
-O, --output-type b|u|z|v
see Common Options
-r, --regions chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-R, --regions-file file
see Common Options
--rename-chrs file
rename chromosomes according to the map in file, with "old_name new_name\n" pairs separated by whitespaces, each on a separate line.
-s, --samples [^]LIST
subset of samples to annotate, see also Common Options
-S, --samples-file FILE
subset of samples to annotate. If the samples are named differently in the target VCF and the -a, --annotations VCF, the name mapping can be given as "src_name dst_name\n", separated by whitespaces, each pair on a separate line.
-x, --remove list
List of annotations to remove. Use "FILTER" to remove all filters or "FILTER/SomeFilter" to remove a specific filter. Similarly, "INFO" can be used to remove all INFO tags and "FORMAT" to remove all FORMAT tags except GT. To remove all INFO tags except "FOO" and "BAR", use "^INFO/FOO,INFO/BAR" (and similarly for FORMAT and FILTER). "INFO" can be abbreviated to "INF" and "FORMAT" to "FMT".

Examples:

    # Remove three fields
    bcftools annotate -x ID,INFO/DP,FORMAT/DP file.vcf.gz

    # Remove all INFO fields and all FORMAT fields except for GT and PL
    bcftools annotate -x INFO,^FORMAT/GT,FORMAT/PL file.vcf

    # Add ID, QUAL and INFO/TAG, not replacing TAG if already present
    bcftools annotate -a src.bcf -c ID,QUAL,+TAG dst.bcf

    # Carry over all INFO and FORMAT annotations except FORMAT/GT
    bcftools annotate -a src.bcf -c INFO,^FORMAT/GT dst.bcf

    # Annotate from a tab-delimited file with six columns (the fifth is ignored),
    # first indexing with tabix. The coordinates are 1-based.
    tabix -s1 -b2 -e2 annots.tab.gz
    bcftools annotate -a annots.tab.gz -h annots.hdr -c CHROM,POS,REF,ALT,-,TAG file.vcf

    # Annotate from a tab-delimited file with regions (1-based coordinates, inclusive)
    tabix -s1 -b2 -e3 annots.tab.gz
    bcftools annotate -a annots.tab.gz -h annots.hdr -c CHROM,FROM,TO,TAG inut.vcf

    # Annotate from a bed file (0-based coordinates, half-closed, half-open intervals)
    bcftools annotate -a annots.bed.gz -h annots.hdr -c CHROM,FROM,TO,TAG input.vcf

bcftools call [OPTIONS] FILE

This command replaces the former bcftools view caller. Some of the original functionality has been temporarily lost in the process of transition under htslib, but will be added back on popular demand. The original calling model can be invoked with the -c option.

File format options:

-O, --output-type b|u|z|v
see Common Options
-r, --regions chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-R, --regions-file file
see Common Options
-s, --samples LIST
see Common Options
-S, --samples-file FILE
see Common Options
-t, --targets LIST
see Common Options
-T, --targets-file FILE
see Common Options

Input/output options:

-A, --keep-alts
output all alternate alleles present in the alignments even if they do not appear in any of the genotypes
-f, --format-fields list
comma-separated list of FORMAT fields to output for each sample. Currently GQ and GP fields are supported. For convenience, the fields can be given as lower case letters.
-g, --gvcf INT
output also gVCF blocks of homozygous REF calls. The parameter INT is the minimum per-sample depth required to include a site in the non-variant block.
-M, --keep-masked-ref
output sites where REF allele is N
-o, --output FILE
see Common Options
-V, --skip-variants snps|indels
skip indel/SNP sites
-v, --variants-only
output variant sites only

Consensus/variant calling options:

-c, --consensus-caller
the original samtools/bcftools calling method (conflicts with -m)
-C, --constrain alleles|trio
alleles
call genotypes given alleles. See also -T, --targets-file.
trio
call genotypes given the father-mother-child constraint. See also -s, --samples and -n, --novel-rate.
-m, --multiallelic-caller
alternative modelfor multiallelic and rare-variant calling designed to overcome known limitations in -c calling model (conflicts with -c)
-n, --novel-rate float[,…]
likelihood of novel mutation for constrained -C trio calling. The trio genotype calling maximizes likelihood of a particular combination of genotypes for father, mother and the child P(F=i,M=j,C=k) = P(unconstrained) * Pn + P(constrained) * (1-Pn). By providing three values, the mutation rate Pn is set explictly for SNPs, deletions and insertions, respectively. If two values are given, the first is interpreted as the mutation rate of SNPs and the second is used to calculate the mutation rate of indels according to their length as Pn=float*exp(-a-b*len), where a=22.8689, b=0.2994 for insertions and a=21.9313, b=0.2856 for deletions [pubmed:23975140]. If only one value is given, the same mutation rate Pn is used for SNPs and indels.
-p, --pval-threshold float
with -c, accept variant if P(ref|D) < float.
-P, --prior float
expected substitution rate, or 0 to disable the prior.
-t, --targets file|chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-X, --chromosome-X
haploid output for male samples (requires PED file with -s)
-Y, --chromosome-Y
haploid output for males and skips females (requires PED file with -s)

bcftools concat [OPTIONS] FILE1 FILE2 […]

Concatenate or combine VCF/BCF files. All source files must have the same sample columns appearing in the same order. Can be used, for example, to concatenate chromosome VCFs into one VCF, or combine a SNP VCF and an indel VCF into one. The input files must be sorted by chr and position. The files must be given in the correct order to produce sorted VCF on output unless the -a, --allow-overlaps option is specified.

-a, --allow-overlaps
First coordinate of the next file can precede last record of the current file.
-D, --remove-duplicates
If a record is present in multiple files, output only the first instance. Requires -a, --allow-overlaps.
-f, --file-list FILE
Read the list of files from a file.
-l, --ligate
Ligate phased VCFs by matching phase at overlapping haplotypes
-o, --output FILE
see Common Options
-O, --output-type b|u|z|v
see Common Options
-q, --min-PQ INT
Break phase set if phasing quality is lower than INT
-r, --regions chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options. Requires -a, --allow-overlaps.
-R, --regions-file FILE
see Common Options. Requires -a, --allow-overlaps.

bcftools consensus [OPTIONS] FILE

Create consensus sequence by applying VCF variants to a reference fasta file.

-f, --fasta-ref FILE
reference sequence in fasta format
-H, --haplotype 1|2
apply variants for the given haplotype. This option requires -s, unless exactly one sample is present in the VCF
-i, --iupac-codes
output variants in the form of IUPAC ambiguity codes
-m, --mask FILE
BED file or TAB file with regions to be replaced with N. See discussion of --regions-file in Common Options for file format details.
-o, --output FILE
write output to a file
-s, --sample NAME
apply variants of the given sample

Examples:

    # Apply variants present in sample "NA001", output IUPAC codes for hets
    bcftools consensus -i -s NA001 -f in.fa in.vcf.gz > out.fa

    # Create consensus for one region. The fasta header lines are then expected
    # in the form ">chr:from-to".
    samtools faidx ref.fa 8:11870-11890 | bcftools consensus in.vcf.gz -o out.fa

bcftools convert [OPTIONS] FILE

VCF input options:

-e, --exclude EXPRESSION
exclude sites for which EXPRESSION is true. For valid expressions see EXPRESSIONS.
-i, --include EXPRESSION
include only sites for which EXPRESSION is true. For valid expressions see EXPRESSIONS.
-r, --regions chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-R, --regions-file FILE
see Common Options
-s, --samples LIST
see Common Options
-S, --samples-file FILE
see Common Options
-t, --targets LIST
see Common Options
-T, --targets-file FILE
see Common Options

VCF output options:

-o, --output FILE
see Common Options
-O, --output-type b|u|z|v
see Common Options

GEN/SAMPLE conversion:

-G, --gensample2vcf prefix or gen-file,sample-file
convert IMPUTE2 output to VCF. The second column must be of the form "CHROM:POS_REF_ALT" to detect possible strand swaps; IMPUTE2 leaves the first one empty ("--") when sites from reference panel are filled in. See also -g below.
-g, --gensample prefix or gen-file,sample-file
convert from VCF to gen/sample format used by IMPUTE2 and SHAPEIT. The columns of .gen file format are ID1,ID2,POS,A,B followed by three genotype probabilities P(AA), P(AB), P(BB) for each sample. In order to prevent strand swaps, the program uses IDs of the form "CHROM:POS_REF_ALT". For example: 
  .gen
  ----
  1:111485207_G_A 1:111485207_G_A 111485207 G A 0 1 0 0 1 0
  1:111494194_C_T 1:111494194_C_T 111494194 C T 0 1 0 0 0 1

  .sample
  -------
  ID_1 ID_2 missing
  0 0 0
  sample1 sample1 0
  sample2 sample2 0
--tag STRING
tag to take values for .gen file: GT,PL,GL,GP

gVCF conversion:

--gvcf2vcf
convert gVCF to VCF, expanding REF blocks into sites. Only sites with FILTER set to "PASS" or "." will be expanded.

HAPS/SAMPLE conversion:

--hapsample2vcf prefix or haps-file,sample-file
convert from haps/sample format to VCF. The columns of .haps file are similar to .gen file above, but there are only two haplotype columns per sample. Note that the first column of the haps file is expected to be in the form "CHR:POS_REF_ALT", for example: 
  .haps
  ----
  1:111485207_G_A rsID1 111485207 G A 0 1 0 0
  1:111494194_C_T rsID2 111494194 C T 0 1 0 0

HAPS/LEGEND/SAMPLE conversion:

-H, --haplegendsample2vcf prefix or haps-file,legend-file,sample-file
convert from haps/legend/sample format used by IMPUTE2 to VCF, see also -h, --hapslegendsample below.
-h, --haplegendsample prefix or haps-file,legend-file,sample-file
convert from VCF to haps/legend/sample format used by IMPUTE2 and SHAPEIT. The columns of .legend file ID,POS,REF,ALT. In order to prevent strand swaps, the program uses IDs of the form "CHROM:POS_REF_ALT". The .sample file is quite basic at the moment with columns for population, group and sex expected to be edited by the user. For example: 
  .haps
  -----
  0 1 0 0 1 0
  0 1 0 0 0 1

  .legend
  -------
  id position a0 a1
  1:111485207_G_A 111485207 G A
  1:111494194_C_T 111494194 C T

  .sample
  -------
  sample population group sex
  sample1 sample1 sample1 2
  sample2 sample2 sample2 2
--haploid2diploid
with -h option converts haploid genotypes to homozygous diploid genotypes. For example, the program will print 0 0 instead of the default 0 -. This is useful for programs which do not handle haploid genotypes correctly.
--vcf-ids
output VCF IDs instead of "CHROM:POS_REF_ALT" IDs

TSV conversion:

--tsv2vcf file
convert from TSV (tab-separated values) format (such as generated by 23andMe) to VCF. The input file fields can be tab- or space- delimited
-c, --columns list
comma-separated list of fields in the input file. In the current version, the fields CHROM, POS, ID, and AA are expected and can appear in arbitrary order, columns which should be ignored in the input file can be indicated by "-". The AA field lists alleles on the forward reference strand, for example "CC" or "CT" for diploid genotypes or "C" for haploid genotypes (sex chromosomes). Insertions and deletions are not supported yet, missing data can be indicated with "--".
-f, --fasta-ref file
reference sequence in fasta format
-s, --samples LIST
list of sample names. See Common Options
-S, --samples-file FILE
file of sample names. See Common Options

Example:

# Convert 23andme results into VCF
bcftools convert -c ID,CHROM,POS,AA -s SampleName -f 23andme-ref.fa --tsv2vcf 23andme.txt -Oz -o out.vcf.gz

bcftools filter [OPTIONS] FILE

Apply fixed-threshold filters.

-e, --exclude EXPRESSION
exclude sites for which EXPRESSION is true. For valid expressions see EXPRESSIONS.
-g, --SnpGap INT
filter SNPs within INT base pairs of an indel. The following example demonstrates the logic of --SnpGap 3 applied on a deletion and an insertion: 
The SNPs at positions 1 and 7 are filtered, positions 0 and 8 are not:
         0123456789
    ref  .G.GT..G..
    del  .A.G-..A..
Here the positions 1 and 6 are filtered, 0 and 7 are not:
         0123-456789
    ref  .G.G-..G..
    ins  .A.GT..A..
-G, --IndelGap INT
filter clusters of indels separated by INT or fewer base pairs allowing only one to pass. The following example demonstrates the logic of --IndelGap 2 applied on a deletion and an insertion: 
The second indel is filtered:
         012345678901
    ref  .GT.GT..GT..
    del  .G-.G-..G-..
And similarly here, the second is filtered:
         01 23 456 78
    ref  .A-.A-..A-..
    ins  .AT.AT..AT..
-i, --include EXPRESSION
include only sites for which EXPRESSION is true. For valid expressions see EXPRESSIONS.
-m, --mode [+x]
define behaviour at sites with existing FILTER annotations. The default mode replaces existing filters of failed sites with a new FILTER string while leaving sites which pass untouched when non-empty and setting to "PASS" when the FILTER string is absent. The "+" mode appends new FILTER strings of failed sites instead of replacing them. The "x" mode resets filters of sites which pass to "PASS". Modes "+" and "x" can both be set.
-o, --output FILE
see Common Options
-O, --output-type b|u|z|v
see Common Options
-r, --regions chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-R, --regions-file file
see Common Options
-s, --soft-filter STRING|+
annotate FILTER column with STRING or, with +, a unique filter name generated by the program ("Filter%d").
-S, --set-GTs .|0
set genotypes of failed samples to missing value (.) or reference allele (0)
-t, --targets chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-T, --targets-file file
see Common Options

bcftools gtcheck [OPTIONS] [-g genotypes.vcf.gz] query.vcf.gz

Checks sample identity or, without -g, multi-sample cross-check is performed.

-a, --all-sites
output for all sites
-g, --genotypes genotypes.vcf.gz
reference genotypes to compare against
-G, --GTs-only INT
use genotypes (GT) instead of genotype likelihoods (PL). When set to 1, reported discordance is the number of non-matching GTs, otherwise the number INT is interpreted as phred-scaled likelihood of unobserved genotypes.
-H, --homs-only
consider only genotypes which are homozygous in both genotypes and query VCF. This may be useful with low coverage data.
-p, --plot PREFIX
produce plots
-r, --regions chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-R, --regions-file file
see Common Options
-s, --query-sample STRING
query sample in query.vcf.gz. By default, the first sample is checked.
-S, --target-sample STRING
target sample in the -g file, used only for plotting, not for analysis
-t, --targets file
see Common Options
-T, --targets-file file
see Common Options

Output files format:

CN, Discordance
Pairwise discordance for all sample pairs is calculated as 
        \sum_s { min_G { PL_a(G) + PL_b(G) } },
where the sum runs over all sites s and G is the the most likely genotype shared by both samples a and b. When PL field is not present, a constant value 99 is used for the unseen genotypes. With -G, the value 1 can be used instead; the discordance value then gives exactly the number of differing genotypes.
SM, Average Discordance
Average discordance between sample a and all other samples.
SM, Average Depth
Average depth at evaluated sites, or 1 if FORMAT/DP field is not present.
SM, Average Number of sites
The average number of sites used to calculate the discordance. In other words, the average number of non-missing PLs/genotypes seen both samples.

bcftools index [OPTIONS] <in.bcf>|<in.vcf.gz>

Creates index for bgzip compressed VCF/BCF files for random access. CSI (coordinate-sorted index) is created by default. The CSI format supports indexing of chromosomes up to length 2^31. TBI (tabix index) index files, which support chromosome lengths up to 2^29, can be created by using the -t/--tbi option or using the tabix program packaged with htslib. When loading an index file, bcftools will try the CSI first and then the TBI.

Indexing options:

-c, --csi
generate CSI-format index for VCF/BCF files [default]
-f, --force
overwrite index if it already exists
-m, --min-shift INT
set minimal interval size for CSI indices to 2^INT; default: 14
-t, --tbi
generate TBI-format index for VCF files

Stats options:

-n, --nrecords
print the number of records based on the CSI or TBI index files
-s, --stats
Print per contig stats based on the CSI or TBI index files. Output format is three tab-delimited columns listing the contig name, contig length (. if unknown) and number of records for the contig. Contigs with zero records are not printed.

bcftools isec [OPTIONS] A.vcf.gz B.vcf.gz […]

Creates intersections, unions and complements of VCF files. Depending on the options, the program can output records from one (or more) files which have (or do not have) corresponding records with the same position in the other files.

-c, --collapse snps|indels|both|all|some|none
see Common Options
-C, --complement
output positions present only in the first file but missing in the others
-e, --exclude -|EXPRESSION
exclude sites for which EXPRESSION is true. If -e (or -i) appears only once, the same filtering expression will be applied to all input files. Otherwise, -e or -i must be given for each input file. To indicate that no filtering should be performed on a file, use "-" in place of EXPRESSION, as shown in the example below. For valid expressions see EXPRESSIONS.
-f, --apply-filters LIST
see Common Options
-i, --include EXPRESSION
include only sites for which EXPRESSION is true. See discussion of -e, --exclude above.
-n, --nfiles [+-=]INT
output positions present in this many (=), this many or more (+), or this many or fewer (-) files
-o, --output FILE
see Common Options. When several files are being output, their names are controlled via -p instead.
-O, --output-type b|u|z|v
see Common Options
-p, --prefix DIR
if given, subset each of the input files accordingly. See also -w.
-r, --regions chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-R, --regions-file file
see Common Options
-t, --targets chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-T, --targets-file file
see Common Options
-w, --write LIST
list of input files to output given as 1-based indices. With -p and no -w, all files are written.

Examples:

Create intersection and complements of two sets saving the output in dir/*

    bcftools isec -p dir A.vcf.gz B.vcf.gz

Filter sites in A and B (but not in C) and create intersection

    bcftools isec -e'MAF<0.01' -i'dbSNP=1' -e- A.vcf.gz B.vcf.gz C.vcf.gz -p dir

Extract and write records from A shared by both A and B using exact allele match

    bcftools isec -p dir -n=2 -w1 A.vcf.gz B.vcf.gz

Extract records private to A or B comparing by position only

    bcftools isec -p dir -n-1 -c all A.vcf.gz B.vcf.gz

bcftools merge [OPTIONS] A.vcf.gz B.vcf.gz […]

Merge multiple VCF/BCF files from non-overlapping sample sets to create one multi-sample file. For example, when merging file A.vcf.gz containing samples S1, S2 and S3 and file B.vcf.gz containing samples S3 and S4, the output file will contain four samples named S1, S2, S3, 2:S3 and S4.

Note that it is responsibility of the user to ensure that the sample names are unique across all files. If they are not, the program will exit with an error unless the option --force-samples is given. The sample names can be also given explicitly using the --print-header and --use-header options.

Note that only records from different files can be merged, never from the same file. For "vertical" merge take a look at bcftools norm instead.

--force-samples
if the merged files contain duplicate samples names, proceed anyway. Duplicate sample names will be resolved by prepending index of the file as it appeared on the command line to the conflicting sample name (see 2:S3 in the above example).
--print-header
print only merged header and exit
--use-header FILE
use the VCF header in the provided text FILE
-f, --apply-filters LIST
see Common Options
-i, --info-rules -|TAG:METHOD[,…]
Rules for merging INFO fields (scalars or vectors) or - to disable the default rules. METHOD is one of sum, avg, min, max, join.
-l, --file-list FILE
read file names from FILE
-m, --merge snps|indels|both|all|none|id
The option controls what types of multiallelic records can be created: 
-m none   ..  no new multiallelics, output multiple records instead
-m snps   ..  allow multiallelic SNP records
-m indels ..  allow multiallelic indel records
-m both   ..  both SNP and indel records can be multiallelic
-m all    ..  SNP records can be merged with indel records
-m id     ..  merge by ID
-o, --output FILE
see Common Options
-O, --output-type b|u|z|v
see Common Options
-r, --regions chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-R, --regions-file file
see Common Options

bcftools norm [OPTIONS] file.vcf.gz

Left-align and normalize indels, check if REF alleles match the reference, split multiallelic sites into multiple rows; recover multiallelics from multiple rows.

-D, --remove-duplicates
remove duplicate lines of the same type
-f, --fasta-ref FILE
reference sequence
-m, --multiallelics â†|+>[snps|indels|both|any]
split multiallelic sites into biallelic records (-) or join biallelic sites into multiallelic records (+). An optional type string can follow which controls variant types which should be split or merged together: If only SNP records should be split or merged, specify snps; if both SNPs and indels should be merged separately into two records, specify both; if SNPs and indels should be merged into a single record, specify any.
-o, --output FILE
see Common Options
-O, --output-type b|u|z|v
see Common Options
-r, --regions chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-R, --regions-file file
see Common Options
-s, --strict-filter
when merging (-m+), merged site is PASS only if all sites being merged PASS
-t, --targets LIST
see Common Options
-T, --targets-file FILE
see Common Options
-w, --site-win INT
maximum distance between two records to consider when locally sorting variants which changed position during the realignment

bcftools plugin NAME [OPTIONS] FILE — [PLUGIN OPTIONS]

VCF input options:

-e, --exclude EXPRESSION
exclude sites for which EXPRESSION is true. For valid expressions see EXPRESSIONS.
-i, --include EXPRESSION
include only sites for which EXPRESSION is true. For valid expressions see EXPRESSIONS.
-r, --regions chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-R, --regions-file file
see Common Options
-t, --targets chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-T, --targets-file file
see Common Options

VCF output options:

-o, --output FILE
see Common Options
-O, --output-type b|u|z|v
see Common Options

Plugin options:

-h, --help
list plugin’s options
-l, --list-plugins
List all available plugins. If not installed systemwide, set the environment variable LD_LIBRARY_PATH (linux) or DYLD_LIBRARY_PATH (Mac OS X) to include directory where libhts.so is located. The BCFTOOLS_PLUGINS environment variable tells the program which directories to search.
-v, --verbose
print debugging information to debug plugin failure

List of plugins coming with the distribution:

counts
a minimal plugin which counts number of SNPs, Indels, and total number of sites.
dosage
print genotype dosage. By default the plugin searches for PL, GL and GT, in that order.
fill-AN-AC
fill INFO fields AN and AC.
fix-ploidy
sets correct ploidy
frameshifts
annotate frameshift indels
missing2ref
sets missing genotypes ("./.") to ref allele ("0/0" or "0|0")
tag2tag
Convert between similar tags, such as GL and GP.
vcf2sex
determine sample sex by checking genotypes in haploid regions

Examples:

# List options common to all plugins
bcftools plugin

# List available plugins
bcftools plugin -l

# One can run plugins in several ways
bcftools plugin counts in.vcf
bcftools +counts in.vcf
cat in.vcf | bcftools +counts

# Print usage information of plugin "dosage"
bcftools +dosage -h

# Replace missing genotypes with 0/0
bcftools +missing2ref in.vcf

# Replace missing genotypes with 0|0
bcftools +missing2ref in.vcf -- -p

Plugins troubleshooting:

Things to check if your plugin does not show up in the bcftools plugin -l output:

  • Run with the -v option for verbose output: bcftools plugin -lv
  • Does the environment variable BCFTOOLS_PLUGINS include the correct path?

  • Are all shared libraries, namely libhts.so, accessible? Verify with

    • on Mac OS X: otool -L your/plugin.so and set DYLD_LIBRARY_PATH if they are not
    • on Linux: ldd your/plugin.so and set LD_LIBRARY_PATH if they are not
  • If not installed systemwide, set the environment variable LD_LIBRARY_PATH (linux) or DYLD_LIBRARY_PATH (mac) to include directory where libhts.so is located.

Plugins API:

// Short description used by 'bcftools plugin -l'
const char *about(void);

// Longer description used by 'bcftools +name -h'
const char *usage(void);

// Called once at startup, allows to initialize local variables.
// Return 1 to suppress normal VCF/BCF header output, -1 on critical
// errors, 0 otherwise.
int init(int argc, char **argv, bcf_hdr_t *in_hdr, bcf_hdr_t *out_hdr);

// Called for each VCF record, return NULL to suppress the output
bcf1_t *process(bcf1_t *rec);

// Called after all lines have been processed to clean up
void destroy(void);

bcftools query [OPTIONS] file.vcf.gz [file.vcf.gz […]]

Extracts fields from VCF or BCF files and outputs them in user-defined format.

-c, --collapse snps|indels|both|all|some|none
see Common Options
-e, --exclude EXPRESSION
exclude sites for which EXPRESSION is true. For valid expressions see EXPRESSIONS.
-f, --format FORMAT
learn by example, see below
-H, --print-header
print header
-i, --include EXPRESSION
include only sites for which EXPRESSION is true. For valid expressions see EXPRESSIONS.
-l, --list-samples
list sample names and exit
-o, --output FILE
see Common Options
-r, --regions chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-R, --regions-file file
see Common Options
-s, --samples LIST
see Common Options
-S, --samples-file FILE
see Common Options
-t, --targets chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-T, --targets-file file
see Common Options
-v, --vcf-list FILE
process multiple VCFs listed in the file

Format:

%CHROM          The CHROM column (similarly also other columns: POS, ID, REF, ALT, QUAL, FILTER)
%INFO/TAG       Any tag in the INFO column
%TYPE           Variant type (REF, SNP, MNP, INDEL, OTHER)
%MASK           Indicates presence of the site in other files (with multiple files)
%TAG{INT}       Curly brackets to subscript vectors (0-based)
%FIRST_ALT      Alias for %ALT{0}
[]              The brackets loop over all samples
%GT             Genotype (e.g. 0/1)
%TGT            Translated genotype (e.g. C/A)
%IUPACGT        Genotype translated to IUPAC ambiguity codes (e.g. M instead of C/A)
%LINE           Prints the whole line
%SAMPLE         Sample name

Examples:

bcftools query -f '%CHROM  %POS  %REF  %ALT{0}\n' file.vcf.gz
bcftools query -f '%CHROM\t%POS\t%REF\t%ALT[\t%SAMPLE=%GT]\n' file.vcf.gz

bcftools reheader [OPTIONS] file.vcf.gz

Modify header of VCF/BCF files, change sample names.

-h, --header FILE
new VCF header
-o, --output FILE
see Common Options
-s, --samples FILE
new sample names, one name per line, in the same order as they appear in the VCF file. Alternatively, only samples which need to be renamed can be listed as "old_name new_name\n" pairs separated by whitespaces, each on separate line.

bcftools roh [OPTIONS] file.vcf.gz

A program for detecting runs of homo/autozygosity. Only bi-allelic sites are considered.

The HMM model:

Notation:
  D  = Data, AZ = autozygosity, HW = Hardy-Weinberg (non-autozygosity),
  f  = non-ref allele frequency

Emission probabilities:
  oAZ = P_i(D|AZ) = (1-f)*P(D|RR) + f*P(D|AA)
  oHW = P_i(D|HW) = (1-f)^2 * P(D|RR) + f^2 * P(D|AA) + 2*f*(1-f)*P(D|RA)

Transition probabilities:
  tAZ = P(AZ|HW)  .. from HW to AZ, the -a parameter
  tHW = P(HW|AZ)  .. from AZ to HW, the -H parameter
  P(AZ|AZ) = 1 - P(HW|AZ) = 1 - tHW
  P(HW|HW) = 1 - P(AZ|HW) = 1 - tAZ

  ci  = P_i(C)  .. probability of cross-over at site i, from genetic map
  AZi = P_i(AZ) .. probability of site i being AZ/non-AZ, scaled so that AZi+HWi = 1
  HWi = P_i(HW)

  P_{i+1}(AZ) = oAZ * max[(1-tHW) * (1-ci) * AZ{i-1} , tAZ * ci * (1-AZ{i-1})]
  P_{i+1}(HW) = oHW * max[(1-tAZ) * (1-ci) * (1-AZ{i-1}) , tHW * ci * AZ{i-1}]

General Options:

--AF-tag TAG
use the specified INFO tag TAG as an allele frequency estimate instead of the defaul AC and AN tags. Sites which do not have TAG will be skipped.
--AF-file FILE
Read allele frequencies from a tab-delimited file containing the columns: CHROM\tPOS\tREF,ALT\tAF. The file can be compressed with bgzip and indexed with tabix -s1 -b2 -e2. Sites which are not present in the FILE or have different reference or alternate allele will be skipped. Note that such a file can be easily created from a VCF using: 
    bcftools query -f'%CHROM\t%POS\t%REF,%ALT\t%INFO/TAG\n' file.vcf | bgzip -c > freqs.tab.gz
-e, --estimate-AF FILE
recalculate INFO/AC and INFO/AN on the fly, using either all samples ("-") or samples listed in FILE. By default, allele frequency is estimated from AC and AN counts which are already present in the INFO field.
-G, --GTs-only FLOAT
use genotypes (FORMAT/GT fields) ignoring genotype likelihoods (FORMAT/PL), setting PL of unseen genotypes to FLOAT. Safe value to use is 30 to account for GT errors.
-I, --skip-indels
skip indels as their genotypes are usually enriched for errors
-m, --genetic-map FILE
genetic map in the format required also by IMPUTE2. Only the first and third column are used (position and Genetic_Map(cM)). The FILE can chromosome name.
-r, --regions chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-R, --regions-file file
see Common Options
-s, --sample name
the name of sample to analyze
-t, --targets chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-T, --targets-file file
see Common Options

HMM Options:

-a, --hw-to-az FLOAT
P(AZ|HW) transition probability from AZ (autozygous) to HW (Hardy-Weinberg) state
-H, --az-to-hw FLOAT
P(HW|AZ) transition probability from HW to AZ state
-V, --viterbi-training
perform Viterbi training to estimate transition probabilities

bcftools stats [OPTIONS] A.vcf.gz [B.vcf.gz]

Parses VCF or BCF and produces text file stats which is suitable for machine processing and can be plotted using plot-vcfstats. When two files are given, the program generates separate stats for intersection and the complements. By default only sites are compared, -s/-S must given to include also sample columns.

-1, --1st-allele-only
consider only 1st allele at multiallelic sites
-c, --collapse snps|indels|both|all|some|none
see Common Options
-d, --depth INT,INT,INT
ranges of depth distribution: min, max, and size of the bin
--debug
produce verbose per-site and per-sample output
-e, --exons file.gz
tab-delimited file with exons for indel frameshifts statistics. The columns of the file are CHR, FROM, TO, with 1-based, inclusive, positions. The file is BGZF-compressed and indexed with tabix 
    tabix -s1 -b2 -e3 file.gz
-f, --apply-filters LIST
see Common Options
-F, --fasta-ref ref.fa
faidx indexed reference sequence file to determine INDEL context
-i, --split-by-ID
collect stats separately for sites which have the ID column set ("known sites") or which do not have the ID column set ("novel sites").
-r, --regions chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-R, --regions-file file
see Common Options
-s, --samples LIST
see Common Options
-S, --samples-file FILE
see Common Options
-t, --targets chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-T, --targets-file file
see Common Options

bcftools view [OPTIONS] file.vcf.gz [REGION […]]

View, subset and filter VCF or BCF files by position and filtering expression. Convert between VCF and BCF. Former bcftools subset.

Output options

-G, --drop-genotypes
drop individual genotype information (after subsetting if -s option is set)
-h, --header-only
output the VCF header only
-H, --no-header
suppress the header in VCF output
-l, --compression-level [0-9]
compression level. 0 stands for uncompressed, 1 for best speed and 9 for best compression.
-O, --output-type b|u|z|v
see Common Options

-o, --output-file FILE: output file name. If not present, the default is to print to standard output (stdout).

-r, --regions chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-R, --regions-file file
see Common Options
-t, --targets chr|chr:pos|chr:from-to|chr:from-[,…]
see Common Options
-T, --targets-file file
see Common Options

Subset options:

-a, --trim-alt-alleles
trim alternate alleles not seen in subset. Type A, G and R INFO and FORMAT fields will also be trimmed
-I, --no-update
do not (re)calculate INFO fields for the subset (currently INFO/AC and INFO/AN)
-s, --samples LIST
see Common Options
-S, --samples-file FILE
see Common Options

Filter options:

-c, --min-ac INT[:nref|:alt1|:minor|:major|:'nonmajor']
minimum allele count (INFO/AC) of sites to be printed. Specifying the type of allele is optional and can be set to non-reference (nref, the default), 1st alternate (alt1), the least frequent (minor), the most frequent (major) or sum of all but the most frequent (nonmajor) alleles.
-C, --max-ac INT[:nref|:alt1|:minor|:'major'|:'nonmajor']
maximum allele count (INFO/AC) of sites to be printed. Specifying the type of allele is optional and can be set to non-reference (nref, the default), 1st alternate (alt1), the least frequent (minor), the most frequent (major) or sum of all but the most frequent (nonmajor) alleles.
-e, --exclude EXPRESSION
exclude sites for which EXPRESSION is true. For valid expressions see EXPRESSIONS.
-f, --apply-filters LIST
see Common Options
-g, --genotype [^][hom|het|miss]
include only sites with one or more homozygous (hom), heterozygous (het) or missing (miss) genotypes. When prefixed with ^, the logic is reversed; thus ^het excludes sites with heterozygous genotypes.
-i, --include EXPRESSION
include sites for which EXPRESSION is true. For valid expressions see EXPRESSIONS.
-k, --known
print known sites only (ID column is not ".")
-m, --min-alleles INT
print sites with at least INT alleles listed in REF and ALT columns
-M, --max-alleles INT
print sites with at most INT alleles listed in REF and ALT columns. Use -m2 -M2 -v snps to only view biallelic SNPs.
-n, --novel
print novel sites only (ID column is ".")
-p, --phased
print sites where all samples are phased. Haploid genotypes are considered phased. Missing genotypes considered unphased unless the phased bit is set.
-P, --exclude-phased
exclude sites where all samples are phased
-q, --min-af FLOAT[:nref|:alt1|:minor|:'major'|:'nonmajor']
minimum allele frequency (INFO/AC / INFO/AN) of sites to be printed. Specifying the type of allele is optional and can be set to non-reference (nref, the default), 1st alternate (alt1), the least frequent (minor), the most frequent (major) or sum of all but the most frequent (nonmajor) alleles.
-Q, --max-af FLOAT[:nref|:alt1|:minor|:'major'|:'nonmajor']
maximum allele frequency (INFO/AC / INFO/AN) of sites to be printed. Specifying the type of allele is optional and can be set to non-reference (nref, the default), 1st alternate (alt1), the least frequent (minor), the most frequent (major) or sum of all but the most frequent (nonmajor) alleles.
-u, --uncalled
print sites without a called genotype
-U, --exclude-uncalled
exclude sites without a called genotype
-v, --types snps|indels|mnps|other
comma-separated list of variant types to select
-V, --exclude-types snps|indels|mnps|other
comma-separated list of variant types to exclude
-x, --private
print sites where only the subset samples carry an non-reference allele
-X, --exclude-private
exclude sites where only the subset samples carry an non-reference allele

EXPRESSIONS

These filtering expressions are accepted by annotate, filter, query and view commands.

Valid expressions may contain:

  • numerical constants, string constants, file names 

    1, 1.0, 1e-4
"String"
@file_name

  • arithmetic operators 

    +,*,-,/

  • comparison operators 

    == (same as =), >, >=, <=, <, !=

  • regex operators "~" and its negation "!~" 

    INFO/HAYSTACK ~ "needle"

  • parentheses 

    (, )

  • logical operators 

    && (same as &), ||,  |

  • INFO tags, FORMAT tags, column names 

    INFO/DP or DP
FORMAT/DV, FMT/DV, or DV
FILTER, QUAL, ID, REF, ALT[0]

  • 1 (or 0) to test the presence (or absence) of a flag 

    FlagA=1 && FlagB=0

  • TYPE for variant type in REF,ALT columns (indel,snp,mnp,ref,other) 

    TYPE="indel" | TYPE="snp"

  • array subscripts, "*" for any field 

    (DP4[0]+DP4[1])/(DP4[2]+DP4[3]) > 0.3
DP4[*] == 0
CSQ[*] ~ "missense_variant.*deleterious"

  • function on FORMAT tags (over samples) and INFO tags (over vector fields) 

    MAX, MIN, AVG, SUM, STRLEN, ABS

  • variables calculated on the fly if not present: number of alternate alleles; number of samples; count of alternate alleles; minor allele count (similar to AC but is always smaller than 0.5); frequency of alternate alleles (AF=AC/AN); frequency of minor alleles (MAF=MAC/AN); number of alleles in called genotypes 

    N_ALT, N_SAMPLES, AC, MAC, AF, MAF, AN

Notes:

  • String comparisons and regular expressions are case-insensitive
  • If the subscript "*" is used in regular expression search, the whole field is treated as one string. For example, the regex STR[*]~"B,C" will be true for the string vector INFO/STR=AB,CD.
  • Variables and function names are case-insensitive, but not tag names. For example, "qual" can be used instead of "QUAL", "strlen()" instead of "STRLEN()" , but not "dp" instead of "DP".

Examples:

MIN(DV)>5
MIN(DV/DP)>0.3
MIN(DP)>10 & MIN(DV)>3
QUAL>10 |  FMT/GQ>10   .. selects only GQ>10 samples
QUAL>10 || FMT/GQ>10   .. selects all samples at QUAL>10 sites
TYPE="snp" && QUAL>=10 && (DP4[2]+DP4[3] > 2)
MIN(DP)>35 && AVG(GQ)>50
ID=@file       .. selects lines with ID present in the file
ID!=@~/file    .. skip lines with ID present in the ~/file
MAF[0]<0.05    .. select rare variants at 5% cutoff

Shell expansion:

Note that expressions must often be quoted because some characters have special meaning in the shell. An example of expression enclosed in single quotes which cause that the whole expression is passed to the program as intended:

bcftools view -i '%ID!="." & MAF[0]<0.01'

Please refer to the documentation of your shell for details.

SCRIPTS AND OPTIONS

plot-vcfstats [OPTIONS] file.vchk […]

Script for processing output of bcftools stats. It can merge results from multiple outputs (useful when running the stats for each chromosome separately), plots graphs and creates a PDF presentation.

-m, --merge
Merge vcfstats files to STDOUT, skip plotting.
-p, --prefix PATH
The output files prefix, add a slash to create new directory.
-P, --no-PDF
Skip the PDF creation step.
-r, --rasterize
Rasterize PDF images for faster rendering.
-s, --sample-names
Use sample names for xticks rather than numeric IDs.
-t, --title STRING
Identify files by these titles in plots. The option can be given multiple times, for each ID in the bcftools stats output. If not present, the script will use abbreviated source file names for the titles.
-T, --main-title STRING
Main title for the PDF.

PERFORMANCE

HTSlib was designed with BCF format in mind. When parsing VCF files, all records are internally converted into BCF representation. Simple operations, like removing a single column from a VCF file, can be therefore done much faster with standard UNIX commands, such as awk or cut. Therefore it is recommended to use BCF as input/output format whenever possible to avoid large overhead of the VCF → BCF → VCF conversion.

BUGS

Please report any bugs you encounter on the github website: http://github.com/samtools/bcftools

AUTHORS

Heng Li from the Sanger Institute wrote the original C version of htslib, samtools and bcftools. Bob Handsaker from the Broad Institute implemented the BGZF library. Petr Danecek, Shane McCarthy and John Marshall are maintaining and further developing bcftools. Many other people contributed to the program and to the file format specifications, both directly and indirectly by providing patches, testing and reporting bugs. We thank them all.

RESOURCES

BCFtools GitHub website: http://github.com/samtools/bcftools

Samtools GitHub website: http://github.com/samtools/samtools

HTSlib GitHub website: http://github.com/samtools/htslib

File format specifications: http://samtools.github.io/hts-specs

BCFtools documentation: http://samtools.github.io/bcftools

BCFtools wiki page: https://github.com/samtools/bcftools/wiki

COPYING

The MIT/Expat License or GPL License, see the LICENSE document for details. Copyright (c) Genome Research Ltd.

bcftools-1.2/doc/bcftools.txt000066400000000000000000001703241246371514100163230ustar00rootroot00000000000000// bcftools.txt -- asciidoc template for the bcftools man page and html. // // Please do not modify bcftools.1 or bcftools.html directly, // edit this file and convert using the following commands: // // make docs // // or // // a2x --doctype manpage --format manpage bcftools.txt // a2x --doctype manpage --format xhtml bcftools.txt // // Contributions are welcome, simply edit this file and send // a pull request or email the modified file directly. // bcftools(1) =========== :doctype: manpage NAME ---- bcftools - utilities for variant calling and manipulating VCFs and BCFs. SYNOPSIS -------- *bcftools* ['COMMAND'] ['OPTIONS'] DESCRIPTION ----------- BCFtools is a set of utilities that manipulate variant calls in the Variant Call Format (VCF) and its binary counterpart BCF. All commands work transparently with both VCFs and BCFs, both uncompressed and BGZF-compressed. Most commands accept VCF, bgzipped VCF and BCF with filetype detected automatically even when streaming from a pipe. Indexed VCF and BCF will work in all situations. Un-indexed VCF and BCF and streams will work in most, but not all situations. BCFtools is designed to work on a stream. It regards an input file "-" as the standard input (stdin) and outputs to the standard output (stdout). Several commands can thus be combined with Unix pipes. === VERSION This manual page was last updated *{date}* and refers to bcftools git version *{version}*. === BCF1 The BCF1 format output by versions of samtools \<= 0.1.19 is *not* compatible with this version of bcftools. To read BCF1 files one can use the view command from old versions of bcftools packaged with samtools versions \<= 0.1.19 to convert to VCF, which can then be read by this version of bcftools. ---- samtools-0.1.19/bcftools/bcftools view file.bcf1 | bcftools view ---- === VARIANT CALLING See 'bcftools call' for variant calling from the output of the 'samtools mpileup' command. In versions of samtools \<= 0.1.19 calling was done with 'bcftools view'. Users are now required to choose between the old samtools calling model ('-c/--consensus-caller') and the new multiallelic calling model ('-m/--multiallelic-caller'). The multiallelic calling model is recommended for most tasks. LIST OF COMMANDS ---------------- For a full list of available commands, run *bcftools* without arguments. For a full list of available options, run *bcftools* 'COMMAND' without arguments. - *<>* .. edit VCF files, add or remove annotations - *<>* .. SNP/indel calling (former "view") - *<>* .. concatenate VCF/BCF files from the same set of samples - *<>* .. create consensus sequence by applying VCF variants - *<>* .. convert VCF/BCF to other formats and back - *<>* .. filter VCF/BCF files using fixed thresholds - *<>* .. check sample concordance, detect sample swaps and contamination - *<>* .. index VCF/BCF - *<>* .. intersections of VCF/BCF files - *<>* .. merge VCF/BCF files files from non-overlapping sample sets - *<>* .. normalize indels - *<>* .. run user-defined plugin - *<>* .. transform VCF/BCF into user-defined formats - *<>* .. modify VCF/BCF header, change sample names - *<>* .. identify runs of homo/auto-zygosity - *<>* .. produce VCF/BCF stats (former vcfcheck) - *<>* .. subset, filter and convert VCF and BCF files LIST OF SCRIPTS --------------- Some helper scripts are bundled with the bcftools code. - *<>* .. plots the output of *<>* COMMANDS AND OPTIONS -------------------- [[common_options]] === Common Options The following options are common to many bcftools commands. See usage for specific commands to see if they apply. 'FILE':: Files can be both VCF or BCF, uncompressed or BGZF-compressed. The file "-" is interpreted as standard input. Some tools may require tabix- or CSI-indexed files. *-c, --collapse* 'snps'|'indels'|'both'|'all'|'some'|'none'|'id':: Controls how to treat records with duplicate positions and defines compatible records across multiple input files. Here by "compatible" we mean records which should be considered as identical by the tools. For example, when performing line intersections, the desire may be to consider as identical all sites with matching positions (*bcftools isec -c* 'all'), or only sites with matching variant type (*bcftools isec -c* 'snps'{nbsp} *-c* 'indels'), or only sites with all alleles identical (*bcftools isec -c* 'none'). 'none';; only records with identical REF and ALT alleles are compatible 'some';; only records where some subset of ALT alleles match are compatible 'all';; all records are compatible, regardless of whether the ALT alleles match or not. In the case of records with the same position, only the first will be considered and appear on output. 'snps';; any SNP records are compatible, regardless of whether the ALT alleles match or not. For duplicate positions, only the first SNP record will be considered and appear on output. 'indels';; all indel records are compatible, regardless of whether the REF and ALT alleles match or not. For duplicate positions, only the first indel record will be considered and appear on output. 'both';; abbreviation of "*-c* 'indels'{nbsp} *-c* 'snps'" 'id';; only records with identical ID column are compatible. Supported by *<>* only. *-f, --apply-filters* 'LIST':: Skip sites where FILTER column does not contain any of the strings listed in 'LIST'. For example, to include only sites which have no filters set, use *-f* '.,PASS'. *-o, --output* 'FILE':: When output consists of a single stream, write it to 'FILE' rather than to standard output, where it is written by default. *-O, --output-type* 'b'|'u'|'z'|'v':: Output compressed BCF ('b'), uncompressed BCF ('u'), compressed VCF ('z'), uncompressed VCF ('v'). *-r, --regions* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: Comma-separated list of regions, see also *-R, --regions-file*. Note that *-r* cannot be used in combination with *-R*. *-R, --regions-file* 'FILE':: Regions can be specified either on command line or in a VCF, BED, or tab-delimited file (the default). The columns of the tab-delimited file are: CHROM, POS, and, optionally, POS_TO, where positions are 1-based and inclusive. Uncompressed files are stored in memory, while bgzip-compressed and tabix-indexed region files are streamed. Note that sequence names must match exactly, "chr20" is not the same as "20". Also note that chromosome ordering in 'FILE' will be respected, the VCF will be processed in the order in which chromosomes first appear in 'FILE'. However, within chromosomes, the VCF will always be processed in ascending genomic coordinate order no matter what order they appear in 'FILE'. Note that overlapping regions in 'FILE' can result in duplicated out of order positions in the output. This option requires indexed VCF/BCF files. Note that *-R* cannot be used in combination with *-r*. *-s, --samples* \[^]'LIST':: Comma-separated list of samples to include or exclude if prefixed with "^". *-S, --samples-file* [^]'FILE':: File of sample names to include or exclude if prefixed with "^". One sample per line. The command *<>* accepts an optional second column indicating ploidy (0, 1 or 2) and can parse also PED files. With *<> -C* 'trio', PED file is expected. *-t, --targets* \[^]'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: Similar as *-r, --regions*, but the next position is accessed by streaming the whole VCF/BCF rather than using the tbi/csi index. Both *-r* and *-t* options can be applied simultaneously: *-r* uses the index to jump to a region and *-t* discards positions which are not in the targets. Unlike *-r*, targets can be prefixed with "^" to request logical complement. For example, "^X,Y,MT" indicates that sequences X, Y and MT should be skipped. Yet another difference between the two is that *-r* checks both start and end positions of indels, whereas *-t* checks start positions only. Note that *-t* cannot be used in combination with *-T*. *-T, --targets-file* \[^]'FILE':: Same *-t, --targets*, but reads regions from a file. Note that *-T* cannot be used in combination with *-t*. :: With the *call -C* 'alleles' command, third column of the targets file must be comma-separated list of alleles, starting with the reference allele. Such a file can be easily created from a VCF using: ---- bcftools query -f'%CHROM\t%POS\t%REF,%ALT\n' file.vcf ---- [[annotate]] === bcftools annotate '[OPTIONS]' 'FILE' This command allows to add or remove annotations. *-a, --annotations* 'file':: Bgzip-compressed and tabix-indexed file with annotations. The file can be VCF, BED, or a tab-delimited file with mandatory columns CHROM, POS (or, alternatively, FROM and TO), optional columns REF and ALT, and arbitrary number of annotation columns. BED files are expected to have the ".bed" or ".bed.gz" suffix (case-insensitive), otherwise a tab-delimited file is assumed. Note that in case of tab-delimited file, the coordinates POS, FROM and TO are one-based and inclusive. When REF and ALT are present, only matching VCF records will be annotated. When multiple ALT alleles are present in the annotation file (given as comma-separated list of alleles), at least one must match one of the alleles in the corresponding VCF record. Similarly, at least one alternate allele from a multi-allelic VCF record must be present in the annotation file. Note that flag types, such as "INFO/FLAG", can be annotated by including a field with the value "1" to set the flag, "0" to remove it, or "." to keep existing flags. See also *-c, --columns* and *-h, --header-lines*. ---- # Sample annotation file with columns CHROM, POS, STRING_TAG, NUMERIC_TAG 1 752566 SomeString 5 1 798959 SomeOtherString 6 # etc. ---- *-c, --columns* 'list':: Comma-separated list of columns or tags to carry over from the annotation file (see also *-a, --annotations*). If the annotation file is not a VCF/BCF, 'list' describes the columns of the annotation file and must include CHROM, POS (or, alternatively, FROM and TO), and optionally REF and ALT. Unused columns which should be ignored can be indicated by "-". If the annotation file is a VCF/BCF, only the edited columns/tags must be present and their order does not matter. The columns ID, QUAL, FILTER, INFO and FORMAT can be edited, where INFO tags can be written both as "INFO/TAG" or simply "TAG", and FORMAT tags can be written as "FORMAT/TAG" or "FMT/TAG". To carry over all INFO annotations, use "INFO". To add all INFO annotations except "TAG", use "^INFO/TAG". By default, existing values are replaced. To add values without overwriting existing annotations, use "+TAG" instead of "TAG". To replace only existing values without modifying missing annotations, use "-TAG". If the annotation file is not a VCF/BCF, all new annotations must be defined via *-h, --header-lines*. *-e, --exclude* 'EXPRESSION':: exclude sites for which 'EXPRESSION' is true. For valid expressions see *<>*. *-h, --header-lines* 'file':: Lines to append to the VCF header, see also *-c, --columns* and *-a, --annotations*. For example: ---- ##INFO= ##INFO= ---- *-I, --set-id* \[+]'FORMAT':: assign ID on the fly. The format is the same as in the *<>* command (see below). By default all existing IDs are replaced. If the format string is preceded by "+", only missing IDs will be set. For example, one can use ---- bcftools annotate --set-id +'%CHROM\_%POS\_%REF\_%FIRST_ALT' file.vcf ---- *-i, --include* 'EXPRESSION':: include only sites for which 'EXPRESSION' is true. For valid expressions see *<>*. *-o, --output* 'FILE':: see *<>* *-O, --output-type* 'b'|'u'|'z'|'v':: see *<>* *-r, --regions* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-R, --regions-file* 'file':: see *<>* *--rename-chrs* 'file':: rename chromosomes according to the map in 'file', with "old_name new_name\n" pairs separated by whitespaces, each on a separate line. *-s, --samples* \[^]'LIST':: subset of samples to annotate, see also *<>* *-S, --samples-file* 'FILE':: subset of samples to annotate. If the samples are named differently in the target VCF and the *-a, --annotations* VCF, the name mapping can be given as "src_name dst_name\n", separated by whitespaces, each pair on a separate line. *-x, --remove* 'list':: List of annotations to remove. Use "FILTER" to remove all filters or "FILTER/SomeFilter" to remove a specific filter. Similarly, "INFO" can be used to remove all INFO tags and "FORMAT" to remove all FORMAT tags except GT. To remove all INFO tags except "FOO" and "BAR", use "^INFO/FOO,INFO/BAR" (and similarly for FORMAT and FILTER). "INFO" can be abbreviated to "INF" and "FORMAT" to "FMT". *Examples:* ---- # Remove three fields bcftools annotate -x ID,INFO/DP,FORMAT/DP file.vcf.gz # Remove all INFO fields and all FORMAT fields except for GT and PL bcftools annotate -x INFO,^FORMAT/GT,FORMAT/PL file.vcf # Add ID, QUAL and INFO/TAG, not replacing TAG if already present bcftools annotate -a src.bcf -c ID,QUAL,+TAG dst.bcf # Carry over all INFO and FORMAT annotations except FORMAT/GT bcftools annotate -a src.bcf -c INFO,^FORMAT/GT dst.bcf # Annotate from a tab-delimited file with six columns (the fifth is ignored), # first indexing with tabix. The coordinates are 1-based. tabix -s1 -b2 -e2 annots.tab.gz bcftools annotate -a annots.tab.gz -h annots.hdr -c CHROM,POS,REF,ALT,-,TAG file.vcf # Annotate from a tab-delimited file with regions (1-based coordinates, inclusive) tabix -s1 -b2 -e3 annots.tab.gz bcftools annotate -a annots.tab.gz -h annots.hdr -c CHROM,FROM,TO,TAG inut.vcf # Annotate from a bed file (0-based coordinates, half-closed, half-open intervals) bcftools annotate -a annots.bed.gz -h annots.hdr -c CHROM,FROM,TO,TAG input.vcf ---- [[call]] === bcftools call '[OPTIONS]' 'FILE' This command replaces the former *bcftools view* caller. Some of the original functionality has been temporarily lost in the process of transition under http://github.com/samtools/htslib[htslib], but will be added back on popular demand. The original calling model can be invoked with the *-c* option. ==== File format options: *-O, --output-type* 'b'|'u'|'z'|'v':: see *<>* *-r, --regions* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-R, --regions-file* 'file':: see *<>* *-s, --samples* 'LIST':: see *<>* *-S, --samples-file* 'FILE':: see *<>* *-t, --targets* 'LIST':: see *<>* *-T, --targets-file* 'FILE':: see *<>* ==== Input/output options: *-A, --keep-alts*:: output all alternate alleles present in the alignments even if they do not appear in any of the genotypes *-f, --format-fields* 'list':: comma-separated list of FORMAT fields to output for each sample. Currently GQ and GP fields are supported. For convenience, the fields can be given as lower case letters. *-g, --gvcf* 'INT':: output also gVCF blocks of homozygous REF calls. The parameter 'INT' is the minimum per-sample depth required to include a site in the non-variant block. *-M, --keep-masked-ref*:: output sites where REF allele is N *-o, --output* 'FILE':: see *<>* *-V, --skip-variants* 'snps'|'indels':: skip indel/SNP sites *-v, --variants-only*:: output variant sites only ==== Consensus/variant calling options: *-c, --consensus-caller*:: the original *samtools*/*bcftools* calling method (conflicts with *-m*) *-C, --constrain* 'alleles'|'trio':: 'alleles';; call genotypes given alleles. See also *-T, --targets-file*. 'trio';; call genotypes given the father-mother-child constraint. See also *-s, --samples* and *-n, --novel-rate*. *-m, --multiallelic-caller*:: alternative modelfor multiallelic and rare-variant calling designed to overcome known limitations in *-c* calling model (conflicts with *-c*) *-n, --novel-rate* 'float'[,...]:: likelihood of novel mutation for constrained *-C* 'trio' calling. The trio genotype calling maximizes likelihood of a particular combination of genotypes for father, mother and the child P(F=i,M=j,C=k) = P(unconstrained) * Pn + P(constrained) * (1-Pn). By providing three values, the mutation rate Pn is set explictly for SNPs, deletions and insertions, respectively. If two values are given, the first is interpreted as the mutation rate of SNPs and the second is used to calculate the mutation rate of indels according to their length as Pn='float'*exp(-a-b*len), where a=22.8689, b=0.2994 for insertions and a=21.9313, b=0.2856 for deletions [pubmed:23975140]. If only one value is given, the same mutation rate Pn is used for SNPs and indels. *-p, --pval-threshold* 'float':: with *-c*, accept variant if P(ref|D) < 'float'. *-P, --prior* 'float':: expected substitution rate, or 0 to disable the prior. *-t, --targets* 'file'|'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-X, --chromosome-X*:: haploid output for male samples (requires PED file with *-s*) *-Y, --chromosome-Y*:: haploid output for males and skips females (requires PED file with *-s*) [[concat]] === bcftools concat '[OPTIONS]' 'FILE1' 'FILE2' [...] Concatenate or combine VCF/BCF files. All source files must have the same sample columns appearing in the same order. Can be used, for example, to concatenate chromosome VCFs into one VCF, or combine a SNP VCF and an indel VCF into one. The input files must be sorted by chr and position. The files must be given in the correct order to produce sorted VCF on output unless the *-a, --allow-overlaps* option is specified. *-a, --allow-overlaps*:: First coordinate of the next file can precede last record of the current file. *-D, --remove-duplicates*:: If a record is present in multiple files, output only the first instance. Requires *-a, --allow-overlaps*. *-f, --file-list* 'FILE':: Read the list of files from a file. *-l, --ligate*:: Ligate phased VCFs by matching phase at overlapping haplotypes *-o, --output* 'FILE':: see *<>* *-O, --output-type* 'b'|'u'|'z'|'v':: see *<>* *-q, --min-PQ* 'INT':: Break phase set if phasing quality is lower than 'INT' *-r, --regions* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>*. Requires *-a, --allow-overlaps*. *-R, --regions-file* 'FILE':: see *<>*. Requires *-a, --allow-overlaps*. [[consensus]] === bcftools consensus '[OPTIONS]' 'FILE' Create consensus sequence by applying VCF variants to a reference fasta file. *-f, --fasta-ref* 'FILE':: reference sequence in fasta format *-H, --haplotype* '1'|'2':: apply variants for the given haplotype. This option requires *-s*, unless exactly one sample is present in the VCF *-i, --iupac-codes*:: output variants in the form of IUPAC ambiguity codes *-m, --mask* 'FILE':: BED file or TAB file with regions to be replaced with N. See discussion of *--regions-file* in *<>* for file format details. *-o, --output* 'FILE':: write output to a file *-s, --sample* 'NAME':: apply variants of the given sample *Examples:* ---- # Apply variants present in sample "NA001", output IUPAC codes for hets bcftools consensus -i -s NA001 -f in.fa in.vcf.gz > out.fa # Create consensus for one region. The fasta header lines are then expected # in the form ">chr:from-to". samtools faidx ref.fa 8:11870-11890 | bcftools consensus in.vcf.gz -o out.fa ---- [[convert]] === bcftools convert '[OPTIONS]' 'FILE' ==== VCF input options: *-e, --exclude* 'EXPRESSION':: exclude sites for which 'EXPRESSION' is true. For valid expressions see *<>*. *-i, --include* 'EXPRESSION':: include only sites for which 'EXPRESSION' is true. For valid expressions see *<>*. *-r, --regions* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-R, --regions-file* 'FILE':: see *<>* *-s, --samples* 'LIST':: see *<>* *-S, --samples-file* 'FILE':: see *<>* *-t, --targets* 'LIST':: see *<>* *-T, --targets-file* 'FILE':: see *<>* ==== VCF output options: *-o, --output* 'FILE':: see *<>* *-O, --output-type* 'b'|'u'|'z'|'v':: see *<>* ==== GEN/SAMPLE conversion: *-G, --gensample2vcf* 'prefix' or 'gen-file','sample-file':: convert IMPUTE2 output to VCF. The second column must be of the form "CHROM:POS_REF_ALT" to detect possible strand swaps; IMPUTE2 leaves the first one empty ("--") when sites from reference panel are filled in. See also *-g* below. *-g, --gensample* 'prefix' or 'gen-file','sample-file':: convert from VCF to gen/sample format used by IMPUTE2 and SHAPEIT. The columns of .gen file format are ID1,ID2,POS,A,B followed by three genotype probabilities P(AA), P(AB), P(BB) for each sample. In order to prevent strand swaps, the program uses IDs of the form "CHROM:POS_REF_ALT". For example: ---- .gen ---- 1:111485207_G_A 1:111485207_G_A 111485207 G A 0 1 0 0 1 0 1:111494194_C_T 1:111494194_C_T 111494194 C T 0 1 0 0 0 1 .sample ------- ID_1 ID_2 missing 0 0 0 sample1 sample1 0 sample2 sample2 0 ---- *--tag* 'STRING':: tag to take values for .gen file: GT,PL,GL,GP ==== gVCF conversion: *--gvcf2vcf*:: convert gVCF to VCF, expanding REF blocks into sites. Only sites with FILTER set to "PASS" or "." will be expanded. ==== HAPS/SAMPLE conversion: *--hapsample2vcf* 'prefix' or 'haps-file','sample-file':: convert from haps/sample format to VCF. The columns of .haps file are similar to .gen file above, but there are only two haplotype columns per sample. Note that the first column of the haps file is expected to be in the form "CHR:POS_REF_ALT", for example: ---- .haps ---- 1:111485207_G_A rsID1 111485207 G A 0 1 0 0 1:111494194_C_T rsID2 111494194 C T 0 1 0 0 ---- ==== HAPS/LEGEND/SAMPLE conversion: *-H, --haplegendsample2vcf* 'prefix' or 'haps-file','legend-file','sample-file':: convert from haps/legend/sample format used by IMPUTE2 to VCF, see also *-h, --hapslegendsample* below. *-h, --haplegendsample* 'prefix' or 'haps-file','legend-file','sample-file':: convert from VCF to haps/legend/sample format used by IMPUTE2 and SHAPEIT. The columns of .legend file ID,POS,REF,ALT. In order to prevent strand swaps, the program uses IDs of the form "CHROM:POS_REF_ALT". The .sample file is quite basic at the moment with columns for population, group and sex expected to be edited by the user. For example: ---- .haps ----- 0 1 0 0 1 0 0 1 0 0 0 1 .legend ------- id position a0 a1 1:111485207_G_A 111485207 G A 1:111494194_C_T 111494194 C T .sample ------- sample population group sex sample1 sample1 sample1 2 sample2 sample2 sample2 2 ---- *--haploid2diploid*:: with *-h* option converts haploid genotypes to homozygous diploid genotypes. For example, the program will print '0 0' instead of the default '0 -'. This is useful for programs which do not handle haploid genotypes correctly. *--vcf-ids*:: output VCF IDs instead of "CHROM:POS_REF_ALT" IDs ==== TSV conversion: *--tsv2vcf* 'file':: convert from TSV (tab-separated values) format (such as generated by 23andMe) to VCF. The input file fields can be tab- or space- delimited *-c, --columns* 'list':: comma-separated list of fields in the input file. In the current version, the fields CHROM, POS, ID, and AA are expected and can appear in arbitrary order, columns which should be ignored in the input file can be indicated by "-". The AA field lists alleles on the forward reference strand, for example "CC" or "CT" for diploid genotypes or "C" for haploid genotypes (sex chromosomes). Insertions and deletions are not supported yet, missing data can be indicated with "--". *-f, --fasta-ref* 'file':: reference sequence in fasta format *-s, --samples* 'LIST':: list of sample names. See *<>* *-S, --samples-file* 'FILE':: file of sample names. See *<>* *Example:* ---- # Convert 23andme results into VCF bcftools convert -c ID,CHROM,POS,AA -s SampleName -f 23andme-ref.fa --tsv2vcf 23andme.txt -Oz -o out.vcf.gz ---- [[filter]] === bcftools filter '[OPTIONS]' 'FILE' Apply fixed-threshold filters. *-e, --exclude* 'EXPRESSION':: exclude sites for which 'EXPRESSION' is true. For valid expressions see *<>*. *-g, --SnpGap* 'INT':: filter SNPs within 'INT' base pairs of an indel. The following example demonstrates the logic of *--SnpGap* '3' applied on a deletion and an insertion: ---- The SNPs at positions 1 and 7 are filtered, positions 0 and 8 are not: 0123456789 ref .G.GT..G.. del .A.G-..A.. Here the positions 1 and 6 are filtered, 0 and 7 are not: 0123-456789 ref .G.G-..G.. ins .A.GT..A.. ---- *-G, --IndelGap* 'INT':: filter clusters of indels separated by 'INT' or fewer base pairs allowing only one to pass. The following example demonstrates the logic of *--IndelGap* '2' applied on a deletion and an insertion: ---- The second indel is filtered: 012345678901 ref .GT.GT..GT.. del .G-.G-..G-.. And similarly here, the second is filtered: 01 23 456 78 ref .A-.A-..A-.. ins .AT.AT..AT.. ---- *-i, --include* 'EXPRESSION':: include only sites for which 'EXPRESSION' is true. For valid expressions see *<>*. *-m, --mode* ['+x']:: define behaviour at sites with existing FILTER annotations. The default mode replaces existing filters of failed sites with a new FILTER string while leaving sites which pass untouched when non-empty and setting to "PASS" when the FILTER string is absent. The "\+" mode appends new FILTER strings of failed sites instead of replacing them. The "x" mode resets filters of sites which pass to "PASS". Modes "+" and "x" can both be set. *-o, --output* 'FILE':: see *<>* *-O, --output-type* 'b'|'u'|'z'|'v':: see *<>* *-r, --regions* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-R, --regions-file* 'file':: see *<>* *-s, --soft-filter* 'STRING'|'+':: annotate FILTER column with 'STRING' or, with '+', a unique filter name generated by the program ("Filter%d"). *-S, --set-GTs* '.'|'0':: set genotypes of failed samples to missing value ('.') or reference allele ('0') *-t, --targets* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-T, --targets-file* 'file':: see *<>* [[gtcheck]] === bcftools gtcheck ['OPTIONS'] [*-g* 'genotypes.vcf.gz'] 'query.vcf.gz' Checks sample identity or, without *-g*, multi-sample cross-check is performed. *-a, --all-sites*:: output for all sites *-g, --genotypes* 'genotypes.vcf.gz':: reference genotypes to compare against *-G, --GTs-only* 'INT':: use genotypes (GT) instead of genotype likelihoods (PL). When set to 1, reported discordance is the number of non-matching GTs, otherwise the number 'INT' is interpreted as phred-scaled likelihood of unobserved genotypes. *-H, --homs-only*:: consider only genotypes which are homozygous in both 'genotypes' and 'query' VCF. This may be useful with low coverage data. *-p, --plot* 'PREFIX':: produce plots *-r, --regions* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-R, --regions-file* 'file':: see *<>* *-s, --query-sample* 'STRING':: query sample in 'query.vcf.gz'. By default, the first sample is checked. *-S, --target-sample* 'STRING':: target sample in the *-g* file, used only for plotting, not for analysis *-t, --targets* 'file':: see *<>* *-T, --targets-file* 'file':: see *<>* ==== Output files format: CN, Discordance;; Pairwise discordance for all sample pairs is calculated as ---- \sum_s { min_G { PL_a(G) + PL_b(G) } }, ---- ;; where the sum runs over all sites 's' and 'G' is the the most likely genotype shared by both samples 'a' and 'b'. When PL field is not present, a constant value '99' is used for the unseen genotypes. With *-G*, the value '1' can be used instead; the discordance value then gives exactly the number of differing genotypes. SM, Average Discordance;; Average discordance between sample 'a' and all other samples. SM, Average Depth;; Average depth at evaluated sites, or 1 if FORMAT/DP field is not present. SM, Average Number of sites;; The average number of sites used to calculate the discordance. In other words, the average number of non-missing PLs/genotypes seen both samples. // MD, Maximum Deviation;; // The maximum absolute deviation from average score of the sample // most dissimilar to the rest. [[index]] === bcftools index ['OPTIONS'] '|' Creates index for bgzip compressed VCF/BCF files for random access. CSI (coordinate-sorted index) is created by default. The CSI format supports indexing of chromosomes up to length 2^31. TBI (tabix index) index files, which support chromosome lengths up to 2^29, can be created by using the '-t/--tbi' option or using the 'tabix' program packaged with htslib. When loading an index file, bcftools will try the CSI first and then the TBI. ==== Indexing options: *-c, --csi*:: generate CSI-format index for VCF/BCF files [default] *-f, --force*:: overwrite index if it already exists *-m, --min-shift 'INT'*:: set minimal interval size for CSI indices to 2^INT; default: 14 *-t, --tbi*:: generate TBI-format index for VCF files ==== Stats options: *-n, --nrecords*:: print the number of records based on the CSI or TBI index files *-s, --stats*:: Print per contig stats based on the CSI or TBI index files. Output format is three tab-delimited columns listing the contig name, contig length ('.' if unknown) and number of records for the contig. Contigs with zero records are not printed. [[isec]] === bcftools isec ['OPTIONS'] 'A.vcf.gz' 'B.vcf.gz' [...] Creates intersections, unions and complements of VCF files. Depending on the options, the program can output records from one (or more) files which have (or do not have) corresponding records with the same position in the other files. *-c, --collapse* 'snps'|'indels'|'both'|'all'|'some'|'none':: see *<>* *-C, --complement*:: output positions present only in the first file but missing in the others *-e, --exclude* '-'|'EXPRESSION':: exclude sites for which 'EXPRESSION' is true. If *-e* (or *-i*) appears only once, the same filtering expression will be applied to all input files. Otherwise, *-e* or *-i* must be given for each input file. To indicate that no filtering should be performed on a file, use "-" in place of 'EXPRESSION', as shown in the example below. For valid expressions see *<>*. *-f, --apply-filters* 'LIST':: see *<>* *-i, --include* 'EXPRESSION':: include only sites for which 'EXPRESSION' is true. See discussion of *-e, --exclude* above. *-n, --nfiles* \[+-=]'INT':: output positions present in this many (=), this many or more (+), or this many or fewer (-) files *-o, --output* 'FILE':: see *<>*. When several files are being output, their names are controlled via *-p* instead. *-O, --output-type* 'b'|'u'|'z'|'v':: see *<>* *-p, --prefix* 'DIR':: if given, subset each of the input files accordingly. See also *-w*. *-r, --regions* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-R, --regions-file* 'file':: see *<>* *-t, --targets* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-T, --targets-file* 'file':: see *<>* *-w, --write* 'LIST':: list of input files to output given as 1-based indices. With *-p* and no *-w*, all files are written. ==== Examples: Create intersection and complements of two sets saving the output in dir/* ---- bcftools isec -p dir A.vcf.gz B.vcf.gz ---- Filter sites in A and B (but not in C) and create intersection ---- bcftools isec -e'MAF<0.01' -i'dbSNP=1' -e- A.vcf.gz B.vcf.gz C.vcf.gz -p dir ---- Extract and write records from A shared by both A and B using exact allele match ---- bcftools isec -p dir -n=2 -w1 A.vcf.gz B.vcf.gz ---- Extract records private to A or B comparing by position only ---- bcftools isec -p dir -n-1 -c all A.vcf.gz B.vcf.gz ---- [[merge]] === bcftools merge ['OPTIONS'] 'A.vcf.gz' 'B.vcf.gz' [...] Merge multiple VCF/BCF files from non-overlapping sample sets to create one multi-sample file. For example, when merging file 'A.vcf.gz' containing samples 'S1', 'S2' and 'S3' and file 'B.vcf.gz' containing samples 'S3' and 'S4', the output file will contain four samples named 'S1', 'S2', 'S3', '2:S3' and 'S4'. Note that it is responsibility of the user to ensure that the sample names are unique across all files. If they are not, the program will exit with an error unless the option *--force-samples* is given. The sample names can be also given explicitly using the *--print-header* and *--use-header* options. Note that only records from different files can be merged, never from the same file. For "vertical" merge take a look at *<>* instead. *--force-samples*:: if the merged files contain duplicate samples names, proceed anyway. Duplicate sample names will be resolved by prepending index of the file as it appeared on the command line to the conflicting sample name (see '2:S3' in the above example). *--print-header*:: print only merged header and exit *--use-header* 'FILE':: use the VCF header in the provided text 'FILE' *-f, --apply-filters* 'LIST':: see *<>* *-i, --info-rules* '-'|'TAG:METHOD'[,...]:: Rules for merging INFO fields (scalars or vectors) or '-' to disable the default rules. 'METHOD' is one of 'sum', 'avg', 'min', 'max', 'join'. *-l, --file-list* 'FILE':: read file names from 'FILE' *-m, --merge* 'snps'|'indels'|'both'|'all'|'none'|'id':: The option controls what types of multiallelic records can be created: ---- -m none .. no new multiallelics, output multiple records instead -m snps .. allow multiallelic SNP records -m indels .. allow multiallelic indel records -m both .. both SNP and indel records can be multiallelic -m all .. SNP records can be merged with indel records -m id .. merge by ID ---- *-o, --output* 'FILE':: see *<>* *-O, --output-type* 'b'|'u'|'z'|'v':: see *<>* *-r, --regions* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-R, --regions-file* 'file':: see *<>* [[norm]] === bcftools norm ['OPTIONS'] 'file.vcf.gz' Left-align and normalize indels, check if REF alleles match the reference, split multiallelic sites into multiple rows; recover multiallelics from multiple rows. *-D, --remove-duplicates*:: remove duplicate lines of the same type *-f, --fasta-ref* 'FILE':: reference sequence *-m, --multiallelics* <-|+>['snps'|'indels'|'both'|'any']:: split multiallelic sites into biallelic records ('-') or join biallelic sites into multiallelic records ('+'). An optional type string can follow which controls variant types which should be split or merged together: If only SNP records should be split or merged, specify 'snps'; if both SNPs and indels should be merged separately into two records, specify 'both'; if SNPs and indels should be merged into a single record, specify 'any'. *-o, --output* 'FILE':: see *<>* *-O, --output-type* 'b'|'u'|'z'|'v':: see *<>* *-r, --regions* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-R, --regions-file* 'file':: see *<>* *-s, --strict-filter*:: when merging ('-m+'), merged site is PASS only if all sites being merged PASS *-t, --targets* 'LIST':: see *<>* *-T, --targets-file* 'FILE':: see *<>* *-w, --site-win* 'INT':: maximum distance between two records to consider when locally sorting variants which changed position during the realignment [[plugin]] === bcftools plugin 'NAME' '[OPTIONS]' 'FILE' -- '[PLUGIN OPTIONS]' ==== VCF input options: *-e, --exclude* 'EXPRESSION':: exclude sites for which 'EXPRESSION' is true. For valid expressions see *<>*. *-i, --include* 'EXPRESSION':: include only sites for which 'EXPRESSION' is true. For valid expressions see *<>*. *-r, --regions* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-R, --regions-file* 'file':: see *<>* *-t, --targets* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-T, --targets-file* 'file':: see *<>* ==== VCF output options: *-o, --output* 'FILE':: see *<>* *-O, --output-type* 'b'|'u'|'z'|'v':: see *<>* ==== Plugin options: *-h, --help*:: list plugin's options *-l, --list-plugins*:: List all available plugins. If not installed systemwide, set the environment variable LD_LIBRARY_PATH (linux) or DYLD_LIBRARY_PATH (Mac OS X) to include directory where *libhts.so* is located. The BCFTOOLS_PLUGINS environment variable tells the program which directories to search. *-v, --verbose*:: print debugging information to debug plugin failure ==== List of plugins coming with the distribution: *counts*:: a minimal plugin which counts number of SNPs, Indels, and total number of sites. *dosage*:: print genotype dosage. By default the plugin searches for PL, GL and GT, in that order. *fill-AN-AC*:: fill INFO fields AN and AC. *fix-ploidy*:: sets correct ploidy *frameshifts*:: annotate frameshift indels *missing2ref*:: sets missing genotypes ("./.") to ref allele ("0/0" or "0|0") *tag2tag*:: Convert between similar tags, such as GL and GP. *vcf2sex*:: determine sample sex by checking genotypes in haploid regions ==== Examples: ---- # List options common to all plugins bcftools plugin # List available plugins bcftools plugin -l # One can run plugins in several ways bcftools plugin counts in.vcf bcftools +counts in.vcf cat in.vcf | bcftools +counts # Print usage information of plugin "dosage" bcftools +dosage -h # Replace missing genotypes with 0/0 bcftools +missing2ref in.vcf # Replace missing genotypes with 0|0 bcftools +missing2ref in.vcf -- -p ---- ==== Plugins troubleshooting: Things to check if your plugin does not show up in the *bcftools plugin -l* output: - Run with the *-v* option for verbose output: *bcftools plugin -lv* - Does the environment variable BCFTOOLS_PLUGINS include the correct path? - Are all shared libraries, namely libhts.so, accessible? Verify with * on Mac OS X: *otool -L your/plugin.so* and set DYLD_LIBRARY_PATH if they are not * on Linux: *ldd your/plugin.so* and set LD_LIBRARY_PATH if they are not - If not installed systemwide, set the environment variable LD_LIBRARY_PATH (linux) or DYLD_LIBRARY_PATH (mac) to include directory where *libhts.so* is located. ==== Plugins API: ---- // Short description used by 'bcftools plugin -l' const char *about(void); // Longer description used by 'bcftools +name -h' const char *usage(void); // Called once at startup, allows to initialize local variables. // Return 1 to suppress normal VCF/BCF header output, -1 on critical // errors, 0 otherwise. int init(int argc, char **argv, bcf_hdr_t *in_hdr, bcf_hdr_t *out_hdr); // Called for each VCF record, return NULL to suppress the output bcf1_t *process(bcf1_t *rec); // Called after all lines have been processed to clean up void destroy(void); ---- [[query]] === bcftools query ['OPTIONS'] 'file.vcf.gz' ['file.vcf.gz' [...]] Extracts fields from VCF or BCF files and outputs them in user-defined format. *-c, --collapse* 'snps'|'indels'|'both'|'all'|'some'|'none':: see *<>* *-e, --exclude* 'EXPRESSION':: exclude sites for which 'EXPRESSION' is true. For valid expressions see *<>*. *-f, --format* 'FORMAT':: learn by example, see below *-H, --print-header*:: print header *-i, --include* 'EXPRESSION':: include only sites for which 'EXPRESSION' is true. For valid expressions see *<>*. *-l, --list-samples*:: list sample names and exit *-o, --output* 'FILE':: see *<>* *-r, --regions* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-R, --regions-file* 'file':: see *<>* *-s, --samples* 'LIST':: see *<>* *-S, --samples-file* 'FILE':: see *<>* *-t, --targets* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-T, --targets-file* 'file':: see *<>* *-v, --vcf-list* 'FILE':: process multiple VCFs listed in the file ==== Format: %CHROM The CHROM column (similarly also other columns: POS, ID, REF, ALT, QUAL, FILTER) %INFO/TAG Any tag in the INFO column %TYPE Variant type (REF, SNP, MNP, INDEL, OTHER) %MASK Indicates presence of the site in other files (with multiple files) %TAG{INT} Curly brackets to subscript vectors (0-based) %FIRST_ALT Alias for %ALT{0} [] The brackets loop over all samples %GT Genotype (e.g. 0/1) %TGT Translated genotype (e.g. C/A) %IUPACGT Genotype translated to IUPAC ambiguity codes (e.g. M instead of C/A) %LINE Prints the whole line %SAMPLE Sample name ==== Examples: bcftools query -f '%CHROM %POS %REF %ALT{0}\n' file.vcf.gz bcftools query -f '%CHROM\t%POS\t%REF\t%ALT[\t%SAMPLE=%GT]\n' file.vcf.gz [[reheader]] === bcftools reheader ['OPTIONS'] 'file.vcf.gz' Modify header of VCF/BCF files, change sample names. *-h, --header* 'FILE':: new VCF header *-o, --output* 'FILE':: see *<>* *-s, --samples* 'FILE':: new sample names, one name per line, in the same order as they appear in the VCF file. Alternatively, only samples which need to be renamed can be listed as "old_name new_name\n" pairs separated by whitespaces, each on separate line. [[roh]] === bcftools roh ['OPTIONS'] 'file.vcf.gz' A program for detecting runs of homo/autozygosity. Only bi-allelic sites are considered. ==== The HMM model: -------------------------------------- Notation: D = Data, AZ = autozygosity, HW = Hardy-Weinberg (non-autozygosity), f = non-ref allele frequency Emission probabilities: oAZ = P_i(D|AZ) = (1-f)*P(D|RR) + f*P(D|AA) oHW = P_i(D|HW) = (1-f)^2 * P(D|RR) + f^2 * P(D|AA) + 2*f*(1-f)*P(D|RA) Transition probabilities: tAZ = P(AZ|HW) .. from HW to AZ, the -a parameter tHW = P(HW|AZ) .. from AZ to HW, the -H parameter P(AZ|AZ) = 1 - P(HW|AZ) = 1 - tHW P(HW|HW) = 1 - P(AZ|HW) = 1 - tAZ ci = P_i(C) .. probability of cross-over at site i, from genetic map AZi = P_i(AZ) .. probability of site i being AZ/non-AZ, scaled so that AZi+HWi = 1 HWi = P_i(HW) P_{i+1}(AZ) = oAZ * max[(1-tHW) * (1-ci) * AZ{i-1} , tAZ * ci * (1-AZ{i-1})] P_{i+1}(HW) = oHW * max[(1-tAZ) * (1-ci) * (1-AZ{i-1}) , tHW * ci * AZ{i-1}] -------------------------------------- ==== General Options: *--AF-tag* 'TAG':: use the specified INFO tag 'TAG' as an allele frequency estimate instead of the defaul AC and AN tags. Sites which do not have 'TAG' will be skipped. *--AF-file* 'FILE':: Read allele frequencies from a tab-delimited file containing the columns: CHROM\tPOS\tREF,ALT\tAF. The file can be compressed with *bgzip* and indexed with tabix -s1 -b2 -e2. Sites which are not present in the 'FILE' or have different reference or alternate allele will be skipped. Note that such a file can be easily created from a VCF using: ---- bcftools query -f'%CHROM\t%POS\t%REF,%ALT\t%INFO/TAG\n' file.vcf | bgzip -c > freqs.tab.gz ---- *-e, --estimate-AF* 'FILE':: recalculate INFO/AC and INFO/AN on the fly, using either all samples ("-") or samples listed in 'FILE'. By default, allele frequency is estimated from AC and AN counts which are already present in the INFO field. *-G, --GTs-only* 'FLOAT':: use genotypes (FORMAT/GT fields) ignoring genotype likelihoods (FORMAT/PL), setting PL of unseen genotypes to 'FLOAT'. Safe value to use is 30 to account for GT errors. *-I, --skip-indels*:: skip indels as their genotypes are usually enriched for errors *-m, --genetic-map* 'FILE':: genetic map in the format required also by IMPUTE2. Only the first and third column are used (position and Genetic_Map(cM)). The 'FILE' can be a single file or a file mask, where string "{CHROM}" is replaced with chromosome name. *-r, --regions* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-R, --regions-file* 'file':: see *<>* *-s, --sample* 'name':: the name of sample to analyze *-t, --targets* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-T, --targets-file* 'file':: see *<>* ==== HMM Options: *-a, --hw-to-az* 'FLOAT':: P(AZ|HW) transition probability from AZ (autozygous) to HW (Hardy-Weinberg) state *-H, --az-to-hw* 'FLOAT':: P(HW|AZ) transition probability from HW to AZ state *-V, --viterbi-training*:: perform Viterbi training to estimate transition probabilities [[stats]] === bcftools stats ['OPTIONS'] 'A.vcf.gz' ['B.vcf.gz'] Parses VCF or BCF and produces text file stats which is suitable for machine processing and can be plotted using *<>*. When two files are given, the program generates separate stats for intersection and the complements. By default only sites are compared, *-s*/*-S* must given to include also sample columns. *-1, --1st-allele-only*:: consider only 1st allele at multiallelic sites *-c, --collapse* 'snps'|'indels'|'both'|'all'|'some'|'none':: see *<>* *-d, --depth* 'INT','INT','INT':: ranges of depth distribution: min, max, and size of the bin *--debug*:: produce verbose per-site and per-sample output *-e, --exons* 'file.gz':: tab-delimited file with exons for indel frameshifts statistics. The columns of the file are CHR, FROM, TO, with 1-based, inclusive, positions. The file is BGZF-compressed and indexed with tabix ---- tabix -s1 -b2 -e3 file.gz ---- *-f, --apply-filters* 'LIST':: see *<>* *-F, --fasta-ref* 'ref.fa':: faidx indexed reference sequence file to determine INDEL context *-i, --split-by-ID*:: collect stats separately for sites which have the ID column set ("known sites") or which do not have the ID column set ("novel sites"). *-r, --regions* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-R, --regions-file* 'file':: see *<>* *-s, --samples* 'LIST':: see *<>* *-S, --samples-file* 'FILE':: see *<>* *-t, --targets* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-T, --targets-file* 'file':: see *<>* [[view]] === bcftools view ['OPTIONS'] 'file.vcf.gz' ['REGION' [...]] View, subset and filter VCF or BCF files by position and filtering expression. Convert between VCF and BCF. Former *bcftools subset*. ==== Output options *-G, --drop-genotypes*:: drop individual genotype information (after subsetting if *-s* option is set) *-h, --header-only*:: output the VCF header only *-H, --no-header*:: suppress the header in VCF output *-l, --compression-level* ['0-9']:: compression level. 0 stands for uncompressed, 1 for best speed and 9 for best compression. *-O, --output-type* 'b'|'u'|'z'|'v':: see *<>* *-o, --output-file* 'FILE': output file name. If not present, the default is to print to standard output (stdout). *-r, --regions* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-R, --regions-file* 'file':: see *<>* *-t, --targets* 'chr'|'chr:pos'|'chr:from-to'|'chr:from-'[,...]:: see *<>* *-T, --targets-file* 'file':: see *<>* ==== Subset options: *-a, --trim-alt-alleles*:: trim alternate alleles not seen in subset. Type A, G and R INFO and FORMAT fields will also be trimmed *-I, --no-update*:: do not (re)calculate INFO fields for the subset (currently INFO/AC and INFO/AN) *-s, --samples* 'LIST':: see *<>* *-S, --samples-file* 'FILE':: see *<>* ==== Filter options: *-c, --min-ac* 'INT'[':nref'|':alt1'|':minor'|':major'|:'nonmajor']:: minimum allele count (INFO/AC) of sites to be printed. Specifying the type of allele is optional and can be set to non-reference ('nref', the default), 1st alternate ('alt1'), the least frequent ('minor'), the most frequent ('major') or sum of all but the most frequent ('nonmajor') alleles. *-C, --max-ac* 'INT'[':nref'|':alt1'|':minor'|:'major'|:'nonmajor']:: maximum allele count (INFO/AC) of sites to be printed. Specifying the type of allele is optional and can be set to non-reference ('nref', the default), 1st alternate ('alt1'), the least frequent ('minor'), the most frequent ('major') or sum of all but the most frequent ('nonmajor') alleles. *-e, --exclude* 'EXPRESSION':: exclude sites for which 'EXPRESSION' is true. For valid expressions see *<>*. *-f, --apply-filters* 'LIST':: see *<>* *-g, --genotype* [^]['hom'|'het'|'miss']:: include only sites with one or more homozygous ('hom'), heterozygous ('het') or missing ('miss') genotypes. When prefixed with '^', the logic is reversed; thus '^het' excludes sites with heterozygous genotypes. *-i, --include* 'EXPRESSION':: include sites for which 'EXPRESSION' is true. For valid expressions see *<>*. *-k, --known*:: print known sites only (ID column is not ".") *-m, --min-alleles* 'INT':: print sites with at least 'INT' alleles listed in REF and ALT columns *-M, --max-alleles* 'INT':: print sites with at most 'INT' alleles listed in REF and ALT columns. Use *-m2 -M2 -v snps* to only view biallelic SNPs. *-n, --novel*:: print novel sites only (ID column is ".") *-p, --phased*:: print sites where all samples are phased. Haploid genotypes are considered phased. Missing genotypes considered unphased unless the phased bit is set. *-P, --exclude-phased*:: exclude sites where all samples are phased *-q, --min-af* 'FLOAT'[':nref'|':alt1'|':minor'|:'major'|:'nonmajor']:: minimum allele frequency (INFO/AC / INFO/AN) of sites to be printed. Specifying the type of allele is optional and can be set to non-reference ('nref', the default), 1st alternate ('alt1'), the least frequent ('minor'), the most frequent ('major') or sum of all but the most frequent ('nonmajor') alleles. *-Q, --max-af* 'FLOAT'[':nref'|':alt1'|':minor'|:'major'|:'nonmajor']:: maximum allele frequency (INFO/AC / INFO/AN) of sites to be printed. Specifying the type of allele is optional and can be set to non-reference ('nref', the default), 1st alternate ('alt1'), the least frequent ('minor'), the most frequent ('major') or sum of all but the most frequent ('nonmajor') alleles. *-u, --uncalled*:: print sites without a called genotype *-U, --exclude-uncalled*:: exclude sites without a called genotype *-v, --types* 'snps'|'indels'|'mnps'|'other':: comma-separated list of variant types to select *-V, --exclude-types* 'snps'|'indels'|'mnps'|'other':: comma-separated list of variant types to exclude *-x, --private*:: print sites where only the subset samples carry an non-reference allele *-X, --exclude-private*:: exclude sites where only the subset samples carry an non-reference allele [[expressions]] EXPRESSIONS ----------- These filtering expressions are accepted by *<>*, *<>*, *<>* and *<>* commands. .Valid expressions may contain: * numerical constants, string constants, file names 1, 1.0, 1e-4 "String" @file_name * arithmetic operators +,*,-,/ * comparison operators == (same as =), >, >=, <=, <, != * regex operators "\~" and its negation "!~" INFO/HAYSTACK ~ "needle" * parentheses (, ) * logical operators && (same as &), ||, | * INFO tags, FORMAT tags, column names INFO/DP or DP FORMAT/DV, FMT/DV, or DV FILTER, QUAL, ID, REF, ALT[0] * 1 (or 0) to test the presence (or absence) of a flag FlagA=1 && FlagB=0 * TYPE for variant type in REF,ALT columns (indel,snp,mnp,ref,other) TYPE="indel" | TYPE="snp" * array subscripts, "*" for any field (DP4[0]+DP4[1])/(DP4[2]+DP4[3]) > 0.3 DP4[*] == 0 CSQ[*] ~ "missense_variant.*deleterious" * function on FORMAT tags (over samples) and INFO tags (over vector fields) MAX, MIN, AVG, SUM, STRLEN, ABS * variables calculated on the fly if not present: number of alternate alleles; number of samples; count of alternate alleles; minor allele count (similar to AC but is always smaller than 0.5); frequency of alternate alleles (AF=AC/AN); frequency of minor alleles (MAF=MAC/AN); number of alleles in called genotypes N_ALT, N_SAMPLES, AC, MAC, AF, MAF, AN .Notes: * String comparisons and regular expressions are case-insensitive * If the subscript "\*" is used in regular expression search, the whole field is treated as one string. For example, the regex STR[*]~"B,C" will be true for the string vector INFO/STR=AB,CD. * Variables and function names are case-insensitive, but not tag names. For example, "qual" can be used instead of "QUAL", "strlen()" instead of "STRLEN()" , but not "dp" instead of "DP". *Examples:* -- MIN(DV)>5 MIN(DV/DP)>0.3 MIN(DP)>10 & MIN(DV)>3 QUAL>10 | FMT/GQ>10 .. selects only GQ>10 samples QUAL>10 || FMT/GQ>10 .. selects all samples at QUAL>10 sites TYPE="snp" && QUAL>=10 && (DP4[2]+DP4[3] > 2) MIN(DP)>35 && AVG(GQ)>50 ID=@file .. selects lines with ID present in the file ID!=@~/file .. skip lines with ID present in the ~/file MAF[0]<0.05 .. select rare variants at 5% cutoff -- *Shell expansion:* Note that expressions must often be quoted because some characters have special meaning in the shell. An example of expression enclosed in single quotes which cause that the whole expression is passed to the program as intended: -- bcftools view -i '%ID!="." & MAF[0]<0.01' -- Please refer to the documentation of your shell for details. SCRIPTS AND OPTIONS ------------------- [[plot-vcfstats]] === plot-vcfstats ['OPTIONS'] 'file.vchk' [...] Script for processing output of *<>*. It can merge results from multiple outputs (useful when running the stats for each chromosome separately), plots graphs and creates a PDF presentation. *-m, --merge*:: Merge vcfstats files to STDOUT, skip plotting. *-p, --prefix* 'PATH':: The output files prefix, add a slash to create new directory. *-P, --no-PDF*:: Skip the PDF creation step. *-r, --rasterize*:: Rasterize PDF images for faster rendering. *-s, --sample-names*:: Use sample names for xticks rather than numeric IDs. *-t, --title* 'STRING':: Identify files by these titles in plots. The option can be given multiple times, for each ID in the *<>* output. If not present, the script will use abbreviated source file names for the titles. *-T, --main-title* 'STRING':: Main title for the PDF. PERFORMANCE ----------- HTSlib was designed with BCF format in mind. When parsing VCF files, all records are internally converted into BCF representation. Simple operations, like removing a single column from a VCF file, can be therefore done much faster with standard UNIX commands, such as *awk* or *cut*. Therefore it is recommended to use BCF as input/output format whenever possible to avoid large overhead of the VCF -> BCF -> VCF conversion. BUGS ---- Please report any bugs you encounter on the github website: AUTHORS ------- Heng Li from the Sanger Institute wrote the original C version of htslib, samtools and bcftools. Bob Handsaker from the Broad Institute implemented the BGZF library. Petr Danecek, Shane McCarthy and John Marshall are maintaining and further developing bcftools. Many other people contributed to the program and to the file format specifications, both directly and indirectly by providing patches, testing and reporting bugs. We thank them all. RESOURCES --------- BCFtools GitHub website: Samtools GitHub website: HTSlib GitHub website: File format specifications: BCFtools documentation: BCFtools wiki page: COPYING ------- The MIT/Expat License or GPL License, see the LICENSE document for details. 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} span.line-through { text-decoration: line-through; } bcftools-1.2/em.c000066400000000000000000000212301246371514100137360ustar00rootroot00000000000000/* em.c -- mathematical functions. Copyright (C) 2010, 2011 Broad Institute. Portions copyright (C) 2013 Genome Research Ltd. Author: Heng Li Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include "kmin.h" #include "call.h" #define ITER_MAX 50 #define ITER_TRY 10 #define EPS 1e-5 extern double kf_gammaq(double, double); /* Generic routines */ // estimate site allele frequency in a very naive and inaccurate way static double est_freq(int n, const double *pdg) { int i, gcnt[3], tmp1; // get a rough estimate of the genotype frequency gcnt[0] = gcnt[1] = gcnt[2] = 0; for (i = 0; i < n; ++i) { const double *p = pdg + i * 3; if (p[0] != 1. || p[1] != 1. || p[2] != 1.) { int which = p[0] > p[1]? 0 : 1; which = p[which] > p[2]? which : 2; ++gcnt[which]; } } tmp1 = gcnt[0] + gcnt[1] + gcnt[2]; return (tmp1 == 0)? -1.0 : (.5 * gcnt[1] + gcnt[2]) / tmp1; } /* Single-locus EM */ typedef struct { int beg, end; const double *pdg; } minaux1_t; static double prob1(double f, void *data) { minaux1_t *a = (minaux1_t*)data; double p = 1., l = 0., f3[3]; int i; // printf("brent %lg\n", f); if (f < 0 || f > 1) return 1e300; f3[0] = (1.-f)*(1.-f); f3[1] = 2.*f*(1.-f); f3[2] = f*f; for (i = a->beg; i < a->end; ++i) { const double *pdg = a->pdg + i * 3; p *= pdg[0] * f3[0] + pdg[1] * f3[1] + pdg[2] * f3[2]; if (p < 1e-200) l -= log(p), p = 1.; } return l - log(p); } // one EM iteration for allele frequency estimate static double freq_iter(double *f, const double *_pdg, int beg, int end) { double f0 = *f, f3[3], err; int i; // printf("em %lg\n", *f); f3[0] = (1.-f0)*(1.-f0); f3[1] = 2.*f0*(1.-f0); f3[2] = f0*f0; for (i = beg, f0 = 0.; i < end; ++i) { const double *pdg = _pdg + i * 3; f0 += (pdg[1] * f3[1] + 2. * pdg[2] * f3[2]) / (pdg[0] * f3[0] + pdg[1] * f3[1] + pdg[2] * f3[2]); } f0 /= (end - beg) * 2; err = fabs(f0 - *f); *f = f0; return err; } /* The following function combines EM and Brent's method. When the signal from * the data is strong, EM is faster but sometimes, EM may converge very slowly. * When this happens, we switch to Brent's method. The idea is learned from * Rasmus Nielsen. */ static double freqml(double f0, int beg, int end, const double *pdg) { int i; double f; for (i = 0, f = f0; i < ITER_TRY; ++i) if (freq_iter(&f, pdg, beg, end) < EPS) break; if (i == ITER_TRY) { // haven't converged yet; try Brent's method minaux1_t a; a.beg = beg; a.end = end; a.pdg = pdg; kmin_brent(prob1, f0 == f? .5*f0 : f0, f, (void*)&a, EPS, &f); } return f; } // one EM iteration for genotype frequency estimate static double g3_iter(double g[3], const double *_pdg, int beg, int end) { double err, gg[3]; int i; gg[0] = gg[1] = gg[2] = 0.; // printf("%lg,%lg,%lg\n", g[0], g[1], g[2]); for (i = beg; i < end; ++i) { double sum, tmp[3]; const double *pdg = _pdg + i * 3; tmp[0] = pdg[0] * g[0]; tmp[1] = pdg[1] * g[1]; tmp[2] = pdg[2] * g[2]; sum = (tmp[0] + tmp[1] + tmp[2]) * (end - beg); gg[0] += tmp[0] / sum; gg[1] += tmp[1] / sum; gg[2] += tmp[2] / sum; } err = fabs(gg[0] - g[0]) > fabs(gg[1] - g[1])? fabs(gg[0] - g[0]) : fabs(gg[1] - g[1]); err = err > fabs(gg[2] - g[2])? err : fabs(gg[2] - g[2]); g[0] = gg[0]; g[1] = gg[1]; g[2] = gg[2]; return err; } // perform likelihood ratio test static double lk_ratio_test(int n, int n1, const double *pdg, double f3[3][3]) { double r; int i; for (i = 0, r = 1.; i < n1; ++i) { const double *p = pdg + i * 3; r *= (p[0] * f3[1][0] + p[1] * f3[1][1] + p[2] * f3[1][2]) / (p[0] * f3[0][0] + p[1] * f3[0][1] + p[2] * f3[0][2]); } for (; i < n; ++i) { const double *p = pdg + i * 3; r *= (p[0] * f3[2][0] + p[1] * f3[2][1] + p[2] * f3[2][2]) / (p[0] * f3[0][0] + p[1] * f3[0][1] + p[2] * f3[0][2]); } return r; } // x[0]: ref frequency // x[1..3]: alt-alt, alt-ref, ref-ref frequenc // x[4]: HWE P-value // x[5..6]: group1 freq, group2 freq // x[7]: 1-degree P-value // x[8]: 2-degree P-value int bcf_em1(call_t *call, const bcf1_t *rec, int n1, int flag, double x[10]) { double *pdg; int i, n; //, n2; if (rec->n_allele < 2) return -1; // one allele only // initialization if (n1 < 0 || n1 > rec->n_sample) n1 = 0; if (flag & 1<<7) flag |= 7<<5; // compute group freq if LRT is required if (flag & 0xf<<1) flag |= 0xf<<1; n = rec->n_sample; //n2 = n - n1; pdg = call->pdg; if (pdg == 0) return -1; for (i = 0; i < 10; ++i) x[i] = -1.; // set to negative { if ((x[0] = est_freq(n, pdg)) < 0.) return -1; // no data x[0] = freqml(x[0], 0, n, pdg); } if (flag & (0xf<<1|3<<8)) { // estimate the genotype frequency and test HWE double *g = x + 1, f3[3], r; f3[0] = g[0] = (1 - x[0]) * (1 - x[0]); f3[1] = g[1] = 2 * x[0] * (1 - x[0]); f3[2] = g[2] = x[0] * x[0]; for (i = 0; i < ITER_MAX; ++i) if (g3_iter(g, pdg, 0, n) < EPS) break; // Hardy-Weinberg equilibrium (HWE) for (i = 0, r = 1.; i < n; ++i) { double *p = pdg + i * 3; r *= (p[0] * g[0] + p[1] * g[1] + p[2] * g[2]) / (p[0] * f3[0] + p[1] * f3[1] + p[2] * f3[2]); } x[4] = kf_gammaq(.5, log(r)); } if ((flag & 7<<5) && n1 > 0 && n1 < n) { // group frequency x[5] = freqml(x[0], 0, n1, pdg); x[6] = freqml(x[0], n1, n, pdg); } if ((flag & 1<<7) && n1 > 0 && n1 < n) { // 1-degree P-value double f[3], f3[3][3], tmp; f[0] = x[0]; f[1] = x[5]; f[2] = x[6]; for (i = 0; i < 3; ++i) f3[i][0] = (1-f[i])*(1-f[i]), f3[i][1] = 2*f[i]*(1-f[i]), f3[i][2] = f[i]*f[i]; tmp = log(lk_ratio_test(n, n1, pdg, f3)); if (tmp < 0) tmp = 0; x[7] = kf_gammaq(.5, tmp); } if ((flag & 3<<8) && n1 > 0 && n1 < n) { // 2-degree P-value double g[3][3], tmp; for (i = 0; i < 3; ++i) memcpy(g[i], x + 1, 3 * sizeof(double)); for (i = 0; i < ITER_MAX; ++i) if (g3_iter(g[1], pdg, 0, n1) < EPS) break; for (i = 0; i < ITER_MAX; ++i) if (g3_iter(g[2], pdg, n1, n) < EPS) break; tmp = log(lk_ratio_test(n, n1, pdg, g)); if (tmp < 0) tmp = 0; x[8] = kf_gammaq(1., tmp); } return 0; } /* Two-locus EM (LD) */ #define _G1(h, k) ((h>>1&1) + (k>>1&1)) #define _G2(h, k) ((h&1) + (k&1)) #if 0 // 0: the previous site; 1: the current site static int pair_freq_iter(int n, double *pdg[2], double f[4]) { double ff[4]; int i, k, h; // printf("%lf,%lf,%lf,%lf\n", f[0], f[1], f[2], f[3]); memset(ff, 0, 4 * sizeof(double)); for (i = 0; i < n; ++i) { double *p[2], sum, tmp; p[0] = pdg[0] + i * 3; p[1] = pdg[1] + i * 3; for (k = 0, sum = 0.; k < 4; ++k) for (h = 0; h < 4; ++h) sum += f[k] * f[h] * p[0][_G1(k,h)] * p[1][_G2(k,h)]; for (k = 0; k < 4; ++k) { tmp = f[0] * (p[0][_G1(0,k)] * p[1][_G2(0,k)] + p[0][_G1(k,0)] * p[1][_G2(k,0)]) + f[1] * (p[0][_G1(1,k)] * p[1][_G2(1,k)] + p[0][_G1(k,1)] * p[1][_G2(k,1)]) + f[2] * (p[0][_G1(2,k)] * p[1][_G2(2,k)] + p[0][_G1(k,2)] * p[1][_G2(k,2)]) + f[3] * (p[0][_G1(3,k)] * p[1][_G2(3,k)] + p[0][_G1(k,3)] * p[1][_G2(k,3)]); ff[k] += f[k] * tmp / sum; } } for (k = 0; k < 4; ++k) f[k] = ff[k] / (2 * n); return 0; } #endif bcftools-1.2/filter.c000066400000000000000000001667551246371514100146500ustar00rootroot00000000000000/* filter.c -- filter expressions. Copyright (C) 2013-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include "filter.h" #include "bcftools.h" #include #include typedef struct _token_t { // read-only values, same for all VCF lines int tok_type; // one of the TOK_* keys below char *key; // set only for string constants, otherwise NULL char *tag; // for debugging and printout only, VCF tag name float threshold; // filtering threshold int hdr_id; // BCF header lookup ID int idx; // 0-based index to VCF vectors, -1: not a vector, -2: any field ([*]) void (*setter)(filter_t *, bcf1_t *, struct _token_t *); int (*comparator)(struct _token_t *, struct _token_t *, int op_type, bcf1_t *); void *hash; // test presence of str value in the hash via comparator regex_t *regex; // precompiled regex for string comparison // modified on filter evaluation at each VCF line float *values; // In case str_value is set, values[0] is one sample's string length char *str_value; // and values[0]*nsamples gives the total length; int is_str; int pass_site; // -1 not applicable, 0 fails, >0 pass uint8_t *pass_samples; // status of individual samples int nsamples; // number of samples int nvalues, mvalues; // number of used values, n=0 for missing values, n=1 for scalars // for strings, total length of str_value } token_t; struct _filter_t { bcf_hdr_t *hdr; char *str; int nfilters; token_t *filters, **flt_stack; // filtering input tokens (in RPN) and evaluation stack int32_t *tmpi; int max_unpack, mtmpi, nsamples; }; #define TOK_VAL 0 #define TOK_LFT 1 // ( #define TOK_RGT 2 // ) #define TOK_LE 3 // less or equal #define TOK_LT 4 // less than #define TOK_EQ 5 // equal #define TOK_BT 6 // bigger than #define TOK_BE 7 // bigger or equal #define TOK_NE 8 // not equal #define TOK_OR 9 // | #define TOK_AND 10 // & #define TOK_ADD 11 // + #define TOK_SUB 12 // - #define TOK_MULT 13 // * #define TOK_DIV 14 // / #define TOK_MAX 15 #define TOK_MIN 16 #define TOK_AVG 17 #define TOK_AND_VEC 18 // && (operator applied in samples) #define TOK_OR_VEC 19 // || (operator applied in samples) #define TOK_LIKE 20 // ~ regular expression #define TOK_NLIKE 21 // !~ regular expression #define TOK_SUM 22 #define TOK_ABS 23 #define TOK_LEN 24 #define TOK_FUNC 25 // 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 // ( ) [ < = > ] ! | & + - * / M m a A O ~ ^ S . l static int op_prec[] = {0,1,1,5,5,5,5,5,5,2,3, 6, 6, 7, 7, 8, 8, 8, 3, 2, 5, 5, 8, 8, 8}; #define TOKEN_STRING "x()[<=>]!|&+-*/MmaAO~^f" static int filters_next_token(char **str, int *len) { char *tmp = *str; while ( *tmp && isspace(*tmp) ) tmp++; *str = tmp; *len = 0; // test for doubles: d.ddde[+-]dd if ( isdigit(*str[0]) || *str[0]=='.' ) // strtod would eat +/- { double HTS_UNUSED v = strtod(*str, &tmp); if ( *str!=tmp && (!tmp[0] || !isalnum(tmp[0])) ) { *len = tmp - (*str); return TOK_VAL; } tmp = *str; } if ( !strncasecmp(tmp,"MAX(",4) ) { (*str) += 3; return TOK_MAX; } if ( !strncasecmp(tmp,"MIN(",4) ) { (*str) += 3; return TOK_MIN; } if ( !strncasecmp(tmp,"AVG(",4) ) { (*str) += 3; return TOK_AVG; } if ( !strncasecmp(tmp,"SUM(",4) ) { (*str) += 3; return TOK_SUM; } if ( !strncasecmp(tmp,"ABS(",4) ) { (*str) += 3; return TOK_ABS; } if ( !strncasecmp(tmp,"STRLEN(",7) ) { (*str) += 6; return TOK_LEN; } if ( !strncasecmp(tmp,"%MAX(",5) ) { (*str) += 4; return TOK_MAX; } // for backward compatibility if ( !strncasecmp(tmp,"%MIN(",5) ) { (*str) += 4; return TOK_MIN; } // for backward compatibility if ( !strncasecmp(tmp,"%AVG(",5) ) { (*str) += 4; return TOK_AVG; } // for backward compatibility if ( !strncasecmp(tmp,"%SUM(",5) ) { (*str) += 4; return TOK_SUM; } // for backward compatibility if ( !strncasecmp(tmp,"INFO/",5) ) tmp += 5; if ( !strncasecmp(tmp,"FORMAT/",7) ) tmp += 7; if ( !strncasecmp(tmp,"FMT/",4) ) tmp += 4; if ( tmp[0]=='@' ) // file name { while ( *tmp && !isspace(*tmp) && *tmp!='=' && *tmp!='!' ) tmp++; *len = tmp - (*str); return TOK_VAL; } while ( tmp[0] ) { if ( tmp[0]=='"' ) break; if ( tmp[0]=='\'' ) break; if ( isspace(tmp[0]) ) break; if ( tmp[0]=='<' ) break; if ( tmp[0]=='>' ) break; if ( tmp[0]=='=' ) break; if ( tmp[0]=='!' ) break; if ( tmp[0]=='&' ) break; if ( tmp[0]=='|' ) break; if ( tmp[0]=='(' ) break; if ( tmp[0]==')' ) break; if ( tmp[0]=='+' ) break; // hacky: so that [*] is not split, the tokenizer does not recognise square brackets [] if ( tmp[0]=='*' && (tmp==*str || tmp[-1]!='[') ) break; if ( tmp[0]=='-' ) break; if ( tmp[0]=='/' ) break; if ( tmp[0]=='~' ) break; tmp++; } if ( tmp > *str ) { *len = tmp - (*str); return TOK_VAL; } if ( tmp[0]=='"' || tmp[0]=='\'' ) { int quote = tmp[0]; tmp++; while ( *tmp && tmp[0]!=quote ) tmp++; if ( !*tmp ) return -1; // missing quotes *len = tmp - (*str) + 1; return TOK_VAL; } if ( tmp[0]=='!' ) { if ( tmp[1]=='=' ) { (*str) += 2; return TOK_NE; } if ( tmp[1]=='~' ) { (*str) += 2; return TOK_NLIKE; } } if ( tmp[0]=='<' ) { if ( tmp[1]=='=' ) { (*str) += 2; return TOK_LE; } (*str) += 1; return TOK_LT; } if ( tmp[0]=='>' ) { if ( tmp[1]=='=' ) { (*str) += 2; return TOK_BE; } (*str) += 1; return TOK_BT; } if ( tmp[0]=='=' ) { if ( tmp[1]=='=' ) { (*str) += 2; return TOK_EQ; } (*str) += 1; return TOK_EQ; } if ( tmp[0]=='(' ) { (*str) += 1; return TOK_LFT; } if ( tmp[0]==')' ) { (*str) += 1; return TOK_RGT; } if ( tmp[0]=='&' && tmp[1]=='&' ) { (*str) += 2; return TOK_AND_VEC; } if ( tmp[0]=='|' && tmp[1]=='|' ) { (*str) += 2; return TOK_OR_VEC; } if ( tmp[0]=='&' ) { (*str) += 1; return TOK_AND; } if ( tmp[0]=='|' ) { (*str) += 1; return TOK_OR; } if ( tmp[0]=='+' ) { (*str) += 1; return TOK_ADD; } if ( tmp[0]=='-' ) { (*str) += 1; return TOK_SUB; } if ( tmp[0]=='*' ) { (*str) += 1; return TOK_MULT; } if ( tmp[0]=='/' ) { (*str) += 1; return TOK_DIV; } if ( tmp[0]=='~' ) { (*str) += 1; return TOK_LIKE; } *len = tmp - (*str); return TOK_VAL; } static void filters_set_qual(filter_t *flt, bcf1_t *line, token_t *tok) { float *ptr = &line->qual; if ( bcf_float_is_missing(*ptr) ) tok->nvalues = 0; else { tok->values[0] = line->qual; tok->nvalues = 1; } } static void filters_set_type(filter_t *flt, bcf1_t *line, token_t *tok) { tok->values[0] = bcf_get_variant_types(line); tok->nvalues = 1; } static void filters_set_info(filter_t *flt, bcf1_t *line, token_t *tok) { assert( tok->hdr_id >=0 ); int i; for (i=0; in_info; i++) if ( line->d.info[i].key == tok->hdr_id ) break; if ( i==line->n_info ) tok->nvalues = 0; else if ( line->d.info[i].type==BCF_BT_CHAR ) { int n = line->d.info[i].len; int m = (int)tok->values[0]; hts_expand(char,n+1,m,tok->str_value); memcpy(tok->str_value,line->d.info[i].vptr,n); tok->str_value[n] = 0; tok->values[0] = m; tok->nvalues = n; } else if ( line->d.info[i].type==BCF_BT_FLOAT ) { tok->values[0] = line->d.info[i].v1.f; tok->str_value = NULL; tok->nvalues = 1; } else { tok->values[0] = line->d.info[i].v1.i; tok->str_value = NULL; tok->nvalues = 1; } } static int filters_cmp_filter(token_t *atok, token_t *btok, int op_type, bcf1_t *line) { int i; if ( op_type==TOK_NE ) // AND logic: none of the filters can match { if ( !line->d.n_flt ) { if ( atok->hdr_id==-1 ) return 0; // missing value return 1; // no filter present, eval to true } for (i=0; id.n_flt; i++) if ( atok->hdr_id==line->d.flt[i] ) return 0; return 1; } // TOK_EQ with OR logic: at least one of the filters must match if ( !line->d.n_flt ) { if ( atok->hdr_id==-1 ) return 1; return 0; // no filter present, eval to false } for (i=0; id.n_flt; i++) if ( atok->hdr_id==line->d.flt[i] ) return 1; return 0; } static int filters_cmp_id(token_t *atok, token_t *btok, int op_type, bcf1_t *line) { // multiple IDs not supported yet (easy to add though) if ( btok->hash ) { token_t *tmp = atok; atok = btok; btok = tmp; } if ( atok->hash ) { int ret = khash_str2int_has_key(atok->hash, line->d.id); if ( op_type==TOK_EQ ) return ret; return ret ? 0 : 1; } if ( op_type==TOK_EQ ) return strcmp(btok->str_value,line->d.id) ? 0 : 1; return strcmp(btok->str_value,line->d.id) ? 1 : 0; } /** * bcf_get_info_value() - get single INFO value, int or float * @line: BCF line * @info_id: tag ID, as returned by bcf_hdr_id2int * @ivec: 0-based index to retrieve, -1 when single value is expected * @vptr: pointer to memory location of sufficient size to accomodate * info_id's type * * The returned value is -1 if tag is not present, 0 if present but * values is missing or ivec is out of range, and 1 on success. */ static int bcf_get_info_value(bcf1_t *line, int info_id, int ivec, void *value) { int j; for (j=0; jn_info; j++) if ( line->d.info[j].key == info_id ) break; if ( j==line->n_info ) return -1; bcf_info_t *info = &line->d.info[j]; if ( info->len == 1 ) { if ( info->type==BCF_BT_FLOAT ) *((float*)value) = info->v1.f; else if ( info->type==BCF_BT_INT8 || info->type==BCF_BT_INT16 || info->type==BCF_BT_INT32 ) *((int*)value) = info->v1.i; return 1; } if ( ivec<0 ) ivec = 0; #define BRANCH(type_t, is_missing, is_vector_end, out_type_t) { \ type_t *p = (type_t *) info->vptr; \ for (j=0; jlen; j++) \ { \ if ( is_vector_end ) return 0; \ } \ if ( is_missing ) return 0; \ *((out_type_t*)value) = p[j]; \ return 1; \ } switch (info->type) { case BCF_BT_INT8: BRANCH(int8_t, p[j]==bcf_int8_missing, p[j]==bcf_int8_vector_end, int); break; case BCF_BT_INT16: BRANCH(int16_t, p[j]==bcf_int16_missing, p[j]==bcf_int16_vector_end, int); break; case BCF_BT_INT32: BRANCH(int32_t, p[j]==bcf_int32_missing, p[j]==bcf_int32_vector_end, int); break; case BCF_BT_FLOAT: BRANCH(float, bcf_float_is_missing(p[j]), bcf_float_is_vector_end(p[j]), float); break; default: fprintf(stderr,"todo: type %d\n", info->type); exit(1); break; } #undef BRANCH return -1; // this shouldn't happen } static void filters_set_info_int(filter_t *flt, bcf1_t *line, token_t *tok) { if ( tok->idx==-2 ) { int i, n = bcf_get_info_int32(flt->hdr,line,tok->tag,&flt->tmpi,&flt->mtmpi); tok->nvalues = n; hts_expand(float,n,tok->mvalues,tok->values); for (i=0; ivalues[i] = flt->tmpi[i]; } else { int32_t value; if ( bcf_get_info_value(line,tok->hdr_id,tok->idx,&value) <= 0 ) tok->nvalues = 0; else { tok->values[0] = value; tok->nvalues = 1; } } } static void filters_set_info_float(filter_t *flt, bcf1_t *line, token_t *tok) { if ( tok->idx==-2 ) { tok->nvalues = bcf_get_info_float(flt->hdr,line,tok->tag,&tok->values,&tok->mvalues); if ( tok->nvalues<0 ) tok->nvalues = 0; } else { float value; if ( bcf_get_info_value(line,tok->hdr_id,tok->idx,&value) <= 0 ) tok->nvalues = 0; else { tok->values[0] = value; tok->nvalues = 1; } } } static void filters_set_info_string(filter_t *flt, bcf1_t *line, token_t *tok) { int m = (int)tok->values[0]; int n = bcf_get_info_string(flt->hdr,line,tok->tag,&tok->str_value,&m); if ( n<0 ) { tok->nvalues = 0; return; } tok->values[0] = m; // allocated length if ( tok->idx>=0 ) { // get ith field (i=tok->idx) int i = 0; char *ss = tok->str_value, *se = tok->str_value + n; while ( ssidx ) { if ( *ss==',' ) i++; ss++; } if ( ss==se || i!=tok->idx ) { tok->nvalues = 0; return; } se = ss; while ( se-tok->str_valuestr_value ) *se = 0; else { memmove(tok->str_value,ss,se-ss); tok->str_value[se-ss] = 0; } tok->nvalues = se-ss; } else if ( tok->idx==-2 ) tok->nvalues = n; } static void filters_set_info_flag(filter_t *flt, bcf1_t *line, token_t *tok) { int j; for (j=0; jn_info; j++) if ( line->d.info[j].key == tok->hdr_id ) break; tok->values[0] = j==line->n_info ? 0 : 1; tok->nvalues = 1; } static void filters_set_format_int(filter_t *flt, bcf1_t *line, token_t *tok) { int i; if ( (tok->nvalues=bcf_get_format_int32(flt->hdr,line,tok->tag,&flt->tmpi,&flt->mtmpi))<0 ) tok->nvalues = 0; else { int is_missing = 1; hts_expand(float,tok->nvalues,tok->mvalues,tok->values); for (i=0; invalues; i++) { if ( flt->tmpi[i]==bcf_int32_missing || flt->tmpi[i]==bcf_int32_vector_end ) bcf_float_set_missing(tok->values[i]); else { tok->values[i] = flt->tmpi[i]; is_missing = 0; } } if ( is_missing ) tok->nvalues = 0; else if ( tok->idx >= 0 ) { int nsmpl = bcf_hdr_nsamples(flt->hdr); int nvals = tok->nvalues / nsmpl; if ( tok->idx >= nvals ) tok->nvalues = 0; // the index is too big else { for (i=0; ivalues[i] = tok->values[i*nvals+tok->idx]; tok->nvalues = nsmpl; } } } tok->nsamples = tok->nvalues; } static void filters_set_format_float(filter_t *flt, bcf1_t *line, token_t *tok) { if ( (tok->nvalues=bcf_get_format_float(flt->hdr,line,tok->tag,&tok->values,&tok->mvalues))<=0 ) tok->nvalues = tok->nsamples = 0; // missing values else if ( tok->idx >= 0 ) { int i, nsmpl, nvals; nsmpl = bcf_hdr_nsamples(flt->hdr); nvals = tok->nvalues / nsmpl; if ( tok->idx >= nvals ) tok->nsamples = tok->nvalues = 0; // the index is too big else { for (i=0; ivalues[i] = tok->values[i*nvals+tok->idx]; tok->nsamples = tok->nvalues = nsmpl; } } } static void filters_set_format_string(filter_t *flt, bcf1_t *line, token_t *tok) { int ndim = tok->nsamples * (int)tok->values[0]; int ret = bcf_get_format_char(flt->hdr,line,tok->tag,&tok->str_value,&ndim); int nsmpl = bcf_hdr_nsamples(flt->hdr); ndim /= nsmpl; tok->values[0] = ndim; if ( ret<=0 ) { tok->nvalues = 0; return; } if ( tok->idx < 0 ) // scalar { tok->nvalues = tok->nsamples = nsmpl; return; } // vector int i; for (i=0; istr_value + i*ndim; int is = 0, ivec = 0; while ( ivecidx && isidx || is==ndim || !ss[is] ) { ss[0] = '.'; ss[1] = 0; continue; } int ie = is; while ( ienvalues = 0; return; } tok->nvalues = ret; tok->nsamples = nsmpl; } static void filters_set_genotype_string(filter_t *flt, bcf1_t *line, token_t *tok) { bcf_fmt_t *fmt = bcf_get_fmt(flt->hdr, line, "GT"); if ( !fmt ) { tok->nvalues = tok->nsamples = 0; return; } int i, blen = 3, nsmpl = bcf_hdr_nsamples(flt->hdr); kstring_t str; str.s = tok->str_value; str.m = tok->values[0] * nsmpl; str.l = 0; for (i=0; invalues = str.l; tok->nsamples = nsmpl; tok->values[0] = blen; tok->str_value = str.s; } static void filters_set_ref_string(filter_t *flt, bcf1_t *line, token_t *tok) { kstring_t str; str.s = tok->str_value; str.m = tok->values[0]; str.l = 0; kputs(line->d.allele[0], &str); tok->nvalues = str.l; tok->values[0] = str.m; tok->str_value = str.s; } static void filters_set_alt_string(filter_t *flt, bcf1_t *line, token_t *tok) { kstring_t str; str.s = tok->str_value; str.m = tok->values[0]; str.l = 0; if ( tok->idx>=0 ) { if ( line->n_allele >= tok->idx ) kputs(line->d.allele[tok->idx], &str); else kputc('.', &str); } else { kputs(line->d.allele[1], &str); int i; for (i=2; in_allele; i++) { kputc(',', &str); kputs(line->d.allele[i], &str); } } tok->nvalues = str.l; tok->values[0] = str.m; tok->str_value = str.s; } static void filters_set_nalt(filter_t *flt, bcf1_t *line, token_t *tok) { tok->nvalues = 1; tok->values[0] = line->n_allele - 1; } static void filters_set_ac(filter_t *flt, bcf1_t *line, token_t *tok) { hts_expand(int32_t, line->n_allele, flt->mtmpi, flt->tmpi); if ( !bcf_calc_ac(flt->hdr, line, flt->tmpi, BCF_UN_INFO|BCF_UN_FMT) ) { tok->nvalues = 0; return; } int i, an = flt->tmpi[0]; for (i=1; in_allele; i++) an += flt->tmpi[i]; if ( !an ) { tok->nvalues = 0; return; } flt->tmpi[0] = an; // for filters_set_[mac|af|maf] if ( tok->idx>=0 ) { tok->nvalues = 1; tok->values[0] = flt->tmpi[tok->idx+1]; } else { hts_expand(float,line->n_allele,tok->mvalues,tok->values); for (i=1; in_allele; i++) tok->values[i-1] = flt->tmpi[i]; tok->nvalues = line->n_allele - 1; } } static void filters_set_an(filter_t *flt, bcf1_t *line, token_t *tok) { filters_set_ac(flt,line,tok); tok->values[0] = tok->nvalues ? flt->tmpi[0] : 0; tok->nvalues = 1; } static void filters_set_mac(filter_t *flt, bcf1_t *line, token_t *tok) { filters_set_ac(flt,line,tok); if ( !tok->nvalues ) return; int i, an = flt->tmpi[0]; for (i=0; invalues; i++) if ( tok->values[i] > an*0.5 ) tok->values[i] = an - tok->values[i]; } static void filters_set_af(filter_t *flt, bcf1_t *line, token_t *tok) { filters_set_ac(flt,line,tok); if ( !tok->nvalues ) return; int i, an = flt->tmpi[0]; for (i=0; invalues; i++) tok->values[i] /= (float)an; } static void filters_set_maf(filter_t *flt, bcf1_t *line, token_t *tok) { filters_set_ac(flt,line,tok); if ( !tok->nvalues ) return; int i, an = flt->tmpi[0]; for (i=0; invalues; i++) { tok->values[i] /= (float)an; if ( tok->values[i] > 0.5 ) tok->values[i] = 1 - tok->values[i]; } } static void set_max(filter_t *flt, bcf1_t *line, token_t *tok) { float val = -HUGE_VAL; int i; for (i=0; invalues; i++) { if ( !bcf_float_is_missing(tok->values[i]) && val < tok->values[i] ) val = tok->values[i]; } tok->values[0] = val; tok->nvalues = 1; tok->nsamples = 0; } static void set_min(filter_t *flt, bcf1_t *line, token_t *tok) { float val = HUGE_VAL; int i; for (i=0; invalues; i++) if ( !bcf_float_is_missing(tok->values[i]) && val > tok->values[i] ) val = tok->values[i]; tok->values[0] = val; tok->nvalues = 1; tok->nsamples = 0; } static void set_avg(filter_t *flt, bcf1_t *line, token_t *tok) { float val = 0; int i, n = 0; for (i=0; invalues; i++) if ( !bcf_float_is_missing(tok->values[i]) ) { val += tok->values[i]; n++; } tok->values[0] = n ? val / n : 0; tok->nvalues = 1; tok->nsamples = 0; } static void set_sum(filter_t *flt, bcf1_t *line, token_t *tok) { float val = 0; int i, n = 0; for (i=0; invalues; i++) if ( !bcf_float_is_missing(tok->values[i]) ) { val += tok->values[i]; n++; } tok->values[0] = val; tok->nvalues = 1; tok->nsamples = 0; } static void set_abs(filter_t *flt, bcf1_t *line, token_t *tok) { if ( tok->is_str ) error("ABS() can be applied only on numeric values\n"); int i; for (i=0; invalues; i++) tok->values[i] = fabs(tok->values[i]); } static void set_strlen(filter_t *flt, bcf1_t *line, token_t *tok) { tok->is_str = 0; if ( !tok->nvalues ) return; if ( tok->idx==-2 ) { int i = 0; char *ss = tok->str_value; while ( *ss ) { char *se = ss; while ( *se && *se!=',' ) se++; if ( !*se ) tok->values[i] = strlen(ss); else { *se = 0; tok->values[i] = strlen(ss); *se = ','; } ss = *se ? se + 1 : se; i++; } tok->nvalues = i; } else { tok->values[0] = strlen(tok->str_value); tok->nvalues = 1; } } #define VECTOR_ARITHMETICS(atok,btok,AOP) \ { \ int i, has_values = 0; \ if ( !(atok)->nvalues || !(btok)->nvalues ) /* missing values */ \ { \ (atok)->nvalues = 0; (atok)->nsamples = 0; \ } \ else \ { \ if ( ((atok)->nsamples && (btok)->nsamples) || (!(atok)->nsamples && !(btok)->nsamples)) \ { \ for (i=0; i<(atok)->nvalues; i++) \ { \ if ( bcf_float_is_missing((atok)->values[i]) ) continue; \ if ( bcf_float_is_missing((btok)->values[i]) ) { bcf_float_set_missing((atok)->values[i]); continue; } \ has_values = 1; \ (atok)->values[i] = (atok)->values[i] AOP (btok)->values[i]; \ } \ } \ else if ( (btok)->nsamples ) \ { \ hts_expand(float,(btok)->nvalues,(atok)->mvalues,(atok)->values); \ for (i=0; i<(btok)->nvalues; i++) \ { \ if ( bcf_float_is_missing((atok)->values[0]) || bcf_float_is_missing((btok)->values[i]) ) \ { \ bcf_float_set_missing((atok)->values[i]); \ continue; \ } \ has_values = 1; \ (atok)->values[i] = (atok)->values[0] AOP (btok)->values[i]; \ } \ (atok)->nvalues = (btok)->nvalues; \ (atok)->nsamples = (btok)->nsamples; \ } \ else if ( (atok)->nsamples ) \ { \ for (i=0; i<(atok)->nvalues; i++) \ { \ if ( bcf_float_is_missing((atok)->values[i]) || bcf_float_is_missing((btok)->values[0]) ) \ { \ bcf_float_set_missing((atok)->values[i]); \ continue; \ } \ has_values = 1; \ (atok)->values[i] = (atok)->values[i] AOP (btok)->values[0]; \ } \ } \ } \ if ( !has_values ) { (atok)->nvalues = 0; (atok)->nsamples = 0; } \ } static int vector_logic_and(token_t *atok, token_t *btok) { // We are comparing either two scalars (result of INFO tag vs a threshold), two vectors (two FORMAT fields), // or a vector and a scalar (FORMAT field vs threshold) int i, pass_site = 0; if ( !atok->nvalues || !btok->nvalues ) { atok->nvalues = atok->nsamples = 0; return 0; } if ( !atok->nsamples && !btok->nsamples ) return atok->pass_site && btok->pass_site; if ( atok->nsamples && btok->nsamples ) { for (i=0; insamples; i++) { atok->pass_samples[i] = atok->pass_samples[i] && btok->pass_samples[i]; if ( !pass_site && atok->pass_samples[i] ) pass_site = 1; } return pass_site; } if ( btok->nsamples ) { for (i=0; insamples; i++) { atok->pass_samples[i] = atok->pass_site && btok->pass_samples[i]; if ( !pass_site && atok->pass_samples[i] ) pass_site = 1; } atok->nsamples = btok->nsamples; return pass_site; } /* atok->nsamples!=0 */ for (i=0; insamples; i++) { atok->pass_samples[i] = atok->pass_samples[i] && btok->pass_site; if ( !pass_site && atok->pass_samples[i] ) pass_site = 1; } return pass_site; } static int vector_logic_or(token_t *atok, token_t *btok, int or_type) { int i, pass_site = 0; if ( !atok->nvalues && !btok->nvalues ) // missing sites in both { atok->nvalues = atok->nsamples = 0; return 0; } if ( !atok->nvalues ) // missing value in a { for (i=0; insamples; i++) atok->pass_samples[i] = btok->pass_samples[i]; atok->nsamples = btok->nsamples; return btok->pass_site; } if ( !btok->nvalues ) // missing value in b return atok->pass_site; if ( !atok->nsamples && !btok->nsamples ) return atok->pass_site || btok->pass_site; if ( !atok->nsamples ) { if ( or_type==TOK_OR ) { for (i=0; insamples; i++) { atok->pass_samples[i] = btok->pass_samples[i]; if ( atok->pass_site || atok->pass_samples[i] ) pass_site = 1; } } else { for (i=0; insamples; i++) { atok->pass_samples[i] = atok->pass_site || btok->pass_samples[i]; if ( atok->pass_samples[i] ) pass_site = 1; } } atok->nsamples = btok->nsamples; return pass_site; } if ( !btok->nsamples ) // vector vs site { if ( or_type==TOK_OR ) { for (i=0; insamples; i++) if ( btok->pass_site || atok->pass_samples[i] ) pass_site = 1; } else { for (i=0; insamples; i++) { atok->pass_samples[i] = atok->pass_samples[i] || btok->pass_site; if ( atok->pass_samples[i] ) pass_site = 1; } } return pass_site; } for (i=0; insamples; i++) { atok->pass_samples[i] = atok->pass_samples[i] || btok->pass_samples[i]; if ( !pass_site && atok->pass_samples[i] ) pass_site = 1; } return pass_site; } #define CMP_VECTORS(atok,btok,CMP_OP,ret) \ { \ int i, j, has_values = 0, pass_site = 0; \ if ( !(atok)->nvalues || !(btok)->nvalues ) { (atok)->nvalues = 0; (atok)->nsamples = 0; (ret) = 0; } \ else \ { \ if ( (atok)->nsamples && (btok)->nsamples ) \ { \ for (i=0; i<(atok)->nsamples; i++) \ { \ if ( bcf_float_is_missing((atok)->values[i]) ) { (atok)->pass_samples[i] = 0; continue; } \ if ( bcf_float_is_missing((btok)->values[i]) ) { (atok)->pass_samples[i] = 0; continue; } \ has_values = 1; \ if ( (atok)->values[i] CMP_OP (btok)->values[i] ) { (atok)->pass_samples[i] = 1; pass_site = 1; } \ else (atok)->pass_samples[i] = 0; \ } \ if ( !has_values ) (atok)->nvalues = 0; \ } \ else if ( (atok)->nsamples ) \ { \ if ( bcf_float_is_missing((btok)->values[0]) ) { (atok)->nvalues = 0; (atok)->nsamples = 0; (ret) = 0; } \ else \ { \ for (i=0; i<(atok)->nsamples; i++) \ { \ if ( bcf_float_is_missing((atok)->values[i]) ) { (atok)->pass_samples[i] = 0; continue; } \ has_values = 1; \ if ( (atok)->values[i] CMP_OP (btok)->values[0] ) { (atok)->pass_samples[i] = 1; pass_site = 1; } \ else (atok)->pass_samples[i] = 0; \ } \ } \ if ( !has_values ) (atok)->nvalues = 0; \ } \ else if ( (btok)->nsamples ) \ { \ if ( bcf_float_is_missing((atok)->values[0]) ) { (atok)->nvalues = 0; (atok)->nsamples = 0; (ret) = 0; } \ else \ { \ for (i=0; i<(btok)->nsamples; i++) \ { \ if ( bcf_float_is_missing((btok)->values[i]) ) { (atok)->pass_samples[i] = 0; continue; } \ has_values = 1; \ if ( (atok)->values[0] CMP_OP (btok)->values[i] ) { (atok)->pass_samples[i] = 1; pass_site = 1; } \ else (atok)->pass_samples[i] = 0; \ } \ (atok)->nvalues = (btok)->nvalues; \ (atok)->nsamples = (btok)->nsamples; \ } \ if ( !has_values ) (atok)->nvalues = 0; \ } \ else if ( (atok)->idx==-2 || (btok)->idx==-2 ) \ { \ /* any field can match: [*] */ \ for (i=0; i<(atok)->nvalues; i++) \ { \ for (j=0; j<(btok)->nvalues; j++) \ if ( (atok)->values[i] CMP_OP (btok)->values[j] ) { pass_site = 1; i = (atok)->nvalues; break; } \ } \ } \ else \ { \ if ( bcf_float_is_missing((atok)->values[0]) || bcf_float_is_missing((btok)->values[0]) ) \ { \ (atok)->nvalues = 0; (atok)->nsamples = 0; (ret) = 0; \ } \ else if ( (atok)->values[0] CMP_OP (btok)->values[0] ) { pass_site = 1; } \ } \ /*fprintf(stderr,"pass=%d\n", pass_site);*/ \ (ret) = pass_site; \ } \ } static int cmp_vector_strings(token_t *atok, token_t *btok, int logic) // logic: TOK_EQ or TOK_NE { if ( !atok->nvalues ) { return 0; } if ( !btok->nvalues ) { atok->nvalues = 0; return 0; } int i, pass_site = 0; if ( atok->nsamples && atok->nsamples==btok->nsamples ) { for (i=0; insamples; i++) { char *astr = atok->str_value + i*(int)atok->values[0]; char *bstr = btok->str_value + i*(int)btok->values[0]; char *aend = astr + (int)atok->values[0], *a = astr; while ( avalues[0], *b = bstr; while ( bpass_samples[i] = 0; else atok->pass_samples[i] = strncmp(astr,bstr,a-astr)==0 ? 1 : 0; if ( logic!=TOK_EQ ) atok->pass_samples[i] = atok->pass_samples[i] ? 0 : 1; pass_site |= atok->pass_samples[i]; } if ( !atok->nsamples ) atok->nsamples = btok->nsamples; } else if ( !atok->nsamples && !btok->nsamples ) { if ( atok->idx==-2 || btok->idx==-2 ) { // any field can match: [*] if ( atok->idx==-2 && btok->idx==-2 ) error("fixme: Expected at least one scalar value [%s %s %s]\n", atok->tag ? atok->tag : btok->tag, atok->str_value,btok->str_value); token_t *xtok, *ytok; // xtok is scalar, ytok array if ( btok->idx==-2 ) { xtok = atok; ytok = btok; } else { xtok = btok; ytok = atok; } char *xstr = xtok->str_value, *xend = xstr + xtok->nvalues; char *ystr = ytok->str_value, *yend = ystr + ytok->nvalues, *y = ystr; while ( y<=yend ) { if ( y==yend || *y==',' ) { if ( y-ystr==xend-xstr && !strncmp(xstr,ystr,xend-xstr) ) { pass_site = 1; break; } ystr = y+1; } y++; } } else pass_site = strcmp(atok->str_value,btok->str_value) ? 0 : 1; if ( logic!=TOK_EQ ) pass_site = pass_site ? 0 : 1; } else { token_t *xtok, *ytok; if ( !atok->nsamples ) { xtok = atok; ytok = btok; } else { xtok = btok; ytok = atok; } char *xstr = xtok->str_value; char *xend = xstr + (int)xtok->values[0], *x = xstr; while ( xnsamples; i++) { char *ystr = ytok->str_value + i*(int)ytok->values[0]; char *yend = ystr + (int)ytok->values[0], *y = ystr; while ( ypass_samples[i] = 0; else atok->pass_samples[i] = strncmp(xstr,ystr,x-xstr)==0 ? 1 : 0; if ( logic!=TOK_EQ ) atok->pass_samples[i] = atok->pass_samples[i] ? 0 : 1; pass_site |= atok->pass_samples[i]; } if ( !atok->nsamples ) atok->nvalues = atok->nsamples = btok->nsamples; // is it a bug? not sure if atok->nvalues should be set } return pass_site; } static int regex_vector_strings(token_t *atok, token_t *btok) { int ret = regexec(btok->regex, atok->str_value, 0,NULL,0); return ret==0 ? 1 : 0; } static int filters_init1(filter_t *filter, char *str, int len, token_t *tok) { tok->tok_type = TOK_VAL; tok->hdr_id = -1; tok->pass_site = -1; tok->idx = -1; // is this a string constant? if ( str[0]=='"' || str[0]=='\'' ) { int quote = str[0]; if ( str[len-1] != quote ) error("TODO: [%s]\n", filter->str); tok->key = (char*) calloc(len-1,sizeof(char)); hts_expand(float,1,tok->mvalues,tok->values); tok->values[0] = len-2; memcpy(tok->key,str+1,len-2); tok->key[len-2] = 0; tok->is_str = 1; tok->nvalues = len-2; return 0; } // is it a file? if ( str[0]=='@' ) { tok->tag = (char*) calloc(len+1,sizeof(char)); memcpy(tok->tag,str,len); tok->tag[len] = 0; wordexp_t wexp; wordexp(tok->tag+1, &wexp, 0); if ( !wexp.we_wordc ) error("No such file: %s\n", tok->tag+1); int i, n; char **list = hts_readlist(wexp.we_wordv[0], 1, &n); if ( !list ) error("Could not read: %s\n", wexp.we_wordv[0]); wordfree(&wexp); tok->hash = khash_str2int_init(); for (i=0; ihash,list[i]) ) khash_str2int_inc(tok->hash,list[i]); else free(list[i]); } free(list); return 0; } int is_fmt = -1; if ( !strncasecmp(str,"FMT/",4) ) { str += 4; len -= 4; is_fmt = 1; } else if ( !strncasecmp(str,"FORMAT/",7) ) { str += 7; len -= 7; is_fmt = 1; } else { if ( !strncasecmp(str,"INFO/",5) ) { is_fmt = 0; str += 5; len -= 5; } else if ( !strncasecmp(str,"QUAL",len) || !strncmp(str,"%QUAL",len) /* for backward compatibility */ ) { tok->setter = filters_set_qual; tok->tag = strdup("QUAL"); return 0; } else if ( !strncasecmp(str,"TYPE",len) || !strncmp(str,"%TYPE",len) /* for backward compatibility */ ) { tok->setter = filters_set_type; tok->tag = strdup("TYPE"); return 0; } else if ( !strncasecmp(str,"FILTER",len) || !strncmp(str,"%FILTER",len) /* for backward compatibility */ ) { tok->comparator = filters_cmp_filter; tok->tag = strdup("FILTER"); filter->max_unpack |= BCF_UN_FLT; return 0; } else if ( !strncasecmp(str,"ID",len) || !strncasecmp(str,"%ID",len) /* for backward compatibility */ ) { tok->comparator = filters_cmp_id; tok->tag = strdup("ID"); return 0; } else if ( !strncasecmp(str,"REF",len) ) { tok->setter = &filters_set_ref_string; tok->is_str = 1; tok->tag = strdup("REF"); return 0; } else if ( !strncasecmp(str,"ALT",len) ) { tok->setter = &filters_set_alt_string; tok->is_str = 1; tok->tag = strdup("ALT"); return 0; } else if ( !strncasecmp(str,"N_ALT",len) ) { tok->setter = &filters_set_nalt; tok->tag = strdup("N_ALT"); return 0; } else if ( !strncasecmp(str,"N_SAMPLES",len) ) { tok->tok_type = TOK_VAL; tok->threshold = bcf_hdr_nsamples(filter->hdr); return 0; } } // does it have array subscript? int is_array = 0; kstring_t tmp = {0,0,0}; kputsn(str, len, &tmp); if ( tmp.s[tmp.l-1] == ']' ) { int i; for (i=0; iidx = -2; // tag[*] .. any field else { char *end; tok->idx = strtol(tmp.s+is_array, &end, 10); if ( *end!=']' ) error("Could not parse the index: %s[%s\n", tmp.s,tmp.s+is_array); } } } tok->hdr_id = bcf_hdr_id2int(filter->hdr,BCF_DT_ID,tmp.s); if ( is_fmt==-1 ) { if ( tok->hdr_id >=0 ) { if ( bcf_hdr_idinfo_exists(filter->hdr,BCF_HL_INFO,tok->hdr_id) ) is_fmt = 0; else if ( bcf_hdr_idinfo_exists(filter->hdr,BCF_HL_FMT,tok->hdr_id) ) is_fmt = 1; } if ( is_fmt==-1 ) is_fmt = 0; } if ( is_fmt ) filter->max_unpack |= BCF_UN_FMT; if ( tok->hdr_id>=0 ) { if ( is_fmt && !strcmp("GT",tmp.s) ) { tok->setter = &filters_set_genotype_string; tok->is_str = 1; } else if ( is_fmt ) { if ( !bcf_hdr_idinfo_exists(filter->hdr,BCF_HL_FMT,tok->hdr_id) ) error("No such FORMAT field: %s\n", tmp.s); if ( bcf_hdr_id2number(filter->hdr,BCF_HL_FMT,tok->hdr_id)!=1 && !is_array ) error("Error: FORMAT vectors must be subscripted, e.g. %s[0] or %s[*]\n", tmp.s, tmp.s); switch ( bcf_hdr_id2type(filter->hdr,BCF_HL_FMT,tok->hdr_id) ) { case BCF_HT_INT: tok->setter = &filters_set_format_int; break; case BCF_HT_REAL: tok->setter = &filters_set_format_float; break; case BCF_HT_STR: tok->setter = &filters_set_format_string; tok->is_str = 1; break; default: error("[%s:%d %s] FIXME\n", __FILE__,__LINE__,__FUNCTION__); } } else if ( !bcf_hdr_idinfo_exists(filter->hdr,BCF_HL_INFO,tok->hdr_id) ) error("No such INFO field: %s\n", tmp.s); else { if ( bcf_hdr_id2type(filter->hdr,BCF_HL_INFO,tok->hdr_id) == BCF_HT_FLAG ) tok->setter = filters_set_info_flag; else { if ( bcf_hdr_id2type(filter->hdr,BCF_HL_INFO,tok->hdr_id) == BCF_HT_STR ) tok->is_str = 1; if ( bcf_hdr_id2number(filter->hdr,BCF_HL_INFO,tok->hdr_id)==1 ) tok->setter = filters_set_info; else { switch ( bcf_hdr_id2type(filter->hdr,BCF_HL_INFO,tok->hdr_id) ) { case BCF_HT_INT: tok->setter = &filters_set_info_int; break; case BCF_HT_REAL: tok->setter = &filters_set_info_float; break; case BCF_HT_STR: tok->setter = &filters_set_info_string; tok->is_str = 1; break; default: error("[%s:%d %s] FIXME\n", __FILE__,__LINE__,__FUNCTION__); } //tok->idx = -2; } } filter->max_unpack |= BCF_UN_INFO; } tok->tag = strdup(tmp.s); if ( tmp.s ) free(tmp.s); return 0; } else if ( !strcasecmp(tmp.s,"ALT") ) { tok->setter = &filters_set_alt_string; tok->is_str = 1; tok->tag = strdup(tmp.s); free(tmp.s); return 0; } else if ( !strcasecmp(tmp.s,"AN") ) { tok->setter = &filters_set_an; tok->tag = strdup("AN"); free(tmp.s); return 0; } else if ( !strcasecmp(tmp.s,"AC") ) { tok->setter = &filters_set_ac; tok->tag = strdup("AC"); free(tmp.s); return 0; } else if ( !strcasecmp(tmp.s,"MAC") ) { tok->setter = &filters_set_mac; tok->tag = strdup("MAC"); free(tmp.s); return 0; } else if ( !strcasecmp(tmp.s,"AF") ) { tok->setter = &filters_set_af; tok->tag = strdup("AF"); free(tmp.s); return 0; } else if ( !strcasecmp(tmp.s,"MAF") ) { tok->setter = &filters_set_maf; tok->tag = strdup("MAF"); free(tmp.s); return 0; } // is it a value? char *end; errno = 0; tok->threshold = strtod(tmp.s, &end); if ( errno!=0 || end!=tmp.s+len ) error("[%s:%d %s] Error: the tag \"INFO/%s\" is not defined in the VCF header\n", __FILE__,__LINE__,__FUNCTION__,tmp.s); if ( tmp.s ) free(tmp.s); return 0; } static void filter_debug_print(token_t *toks, token_t **tok_ptrs, int ntoks) { int i; for (i=0; itok_type==TOK_VAL ) { if ( tok->key ) fprintf(stderr,"%s", tok->key); else if ( tok->tag ) fprintf(stderr,"%s", tok->tag); else fprintf(stderr,"%e", tok->threshold); } else fprintf(stderr,"%c", TOKEN_STRING[tok->tok_type]); if ( tok->setter ) fprintf(stderr,"\t[setter %p]", tok->setter); fprintf(stderr,"\n"); } } // Parse filter expression and convert to reverse polish notation. Dijkstra's shunting-yard algorithm filter_t *filter_init(bcf_hdr_t *hdr, const char *str) { filter_t *filter = (filter_t *) calloc(1,sizeof(filter_t)); filter->str = strdup(str); filter->hdr = hdr; filter->max_unpack |= BCF_UN_STR; int nops = 0, mops = 0, *ops = NULL; // operators stack int nout = 0, mout = 0; // filter tokens, RPN token_t *out = NULL; char *tmp = filter->str; int last_op = -1; while ( *tmp ) { int len, ret; ret = filters_next_token(&tmp, &len); if ( ret==-1 ) error("Missing quotes in: %s\n", str); //fprintf(stderr,"token=[%c] .. [%s] %d\n", TOKEN_STRING[ret], tmp, len); //int i; for (i=0; i0 && ops[nops-1]!=TOK_LFT ) { nout++; hts_expand0(token_t, nout, mout, out); out[nout-1].tok_type = ops[nops-1]; nops--; } if ( nops<=0 ) error("Could not parse: %s\n", str); nops--; } else if ( ret!=TOK_VAL ) // one of the operators { // detect unary minus: replace -value with -1*(value) if ( ret==TOK_SUB && last_op!=TOK_VAL && last_op!=TOK_RGT ) { nout++; hts_expand0(token_t, nout, mout, out); token_t *tok = &out[nout-1]; tok->tok_type = TOK_VAL; tok->hdr_id = -1; tok->pass_site = -1; tok->threshold = -1.0; ret = TOK_MULT; } else { while ( nops>0 && op_prec[ret] < op_prec[ops[nops-1]] ) { nout++; hts_expand0(token_t, nout, mout, out); out[nout-1].tok_type = ops[nops-1]; nops--; } } nops++; hts_expand(int, nops, mops, ops); ops[nops-1] = ret; } else if ( !len ) { if ( *tmp && !isspace(*tmp) ) error("Could not parse the expression: [%s]\n", str); break; // all tokens read } else // annotation name or filtering value { nout++; hts_expand0(token_t, nout, mout, out); filters_init1(filter, tmp, len, &out[nout-1]); tmp += len; } last_op = ret; } while ( nops>0 ) { if ( ops[nops-1]==TOK_LFT || ops[nops-1]==TOK_RGT ) error("Could not parse the expression: [%s]\n", filter->str); nout++; hts_expand0(token_t, nout, mout, out); out[nout-1].tok_type = ops[nops-1]; nops--; } // In the special cases of TYPE and FILTER the BCF header IDs are yet unknown. Walk through the // list of operators and convert the strings (e.g. "PASS") to BCF ids. The string value token must be // just before or after the FILTER token and they must be followed with a comparison operator. // At this point we also initialize regex expressions which, in RPN, must preceed the LIKE/NLIKE operator. // This code is fragile: improve me. int i; for (i=0; istr); out[j].regex = (regex_t *) malloc(sizeof(regex_t)); if ( regcomp(out[j].regex, out[j].key, REG_ICASE|REG_NOSUB) ) error("Could not compile the regex expression \"%s\": %s\n", out[j].key,filter->str); } if ( out[i].tok_type!=TOK_VAL ) continue; if ( !out[i].tag ) continue; if ( !strcmp(out[i].tag,"TYPE") ) { if ( i+1==nout ) error("Could not parse the expression: %s\n", filter->str); int j = i+1; if ( out[j].tok_type==TOK_EQ || out[j].tok_type==TOK_NE ) j = i - 1; if ( out[j].tok_type!=TOK_VAL || !out[j].key ) error("[%s:%d %s] Could not parse the expression: %s\n", __FILE__,__LINE__,__FUNCTION__, filter->str); if ( !strcasecmp(out[j].key,"snp") || !strcasecmp(out[j].key,"snps") ) { out[j].threshold = VCF_SNP; out[j].is_str = 0; } else if ( !strcasecmp(out[j].key,"indel") || !strcasecmp(out[j].key,"indels") ) { out[j].threshold = VCF_INDEL; out[j].is_str = 0; } else if ( !strcasecmp(out[j].key,"mnp") || !strcasecmp(out[j].key,"mnps") ) { out[j].threshold = VCF_MNP; out[j].is_str = 0; } else if ( !strcasecmp(out[j].key,"other") ) { out[j].threshold = VCF_OTHER; out[j].is_str = 0; } else if ( !strcasecmp(out[j].key,"ref") ) { out[j].threshold = VCF_REF; out[j].is_str = 0; } else error("The type \"%s\" not recognised: %s\n", out[j].key, filter->str); out[j].tag = out[j].key; out[j].key = NULL; i = j; continue; } if ( !strcmp(out[i].tag,"FILTER") ) { if ( i+1==nout ) error("Could not parse the expression: %s\n", filter->str); int j = i+1; if ( out[j].tok_type==TOK_EQ || out[j].tok_type==TOK_NE || out[j].tok_type==TOK_LIKE ) j = i - 1; if ( out[j].tok_type!=TOK_VAL || !out[j].key ) error("[%s:%d %s] Could not parse the expression, an unquoted string value perhaps? %s\n", __FILE__,__LINE__,__FUNCTION__, filter->str); if ( strcmp(".",out[j].key) ) { out[j].hdr_id = bcf_hdr_id2int(filter->hdr, BCF_DT_ID, out[j].key); if ( !bcf_hdr_idinfo_exists(filter->hdr,BCF_HL_FLT,out[j].hdr_id) ) error("The filter \"%s\" not present in the VCF header\n", out[j].key); } else out[j].hdr_id = -1; out[j].tag = out[j].key; out[j].key = NULL; out[i].hdr_id = out[j].hdr_id; i = j; continue; } } filter->nsamples = filter->max_unpack&BCF_UN_FMT ? bcf_hdr_nsamples(filter->hdr) : 0; for (i=0; insamples ) { out[i].pass_samples = (uint8_t*)malloc(filter->nsamples); int j; for (j=0; jnsamples; j++) out[i].pass_samples[j] = 1; } } if (0) filter_debug_print(out, NULL, nout); if ( mops ) free(ops); filter->filters = out; filter->nfilters = nout; filter->flt_stack = (token_t **)malloc(sizeof(token_t*)*nout); return filter; } void filter_destroy(filter_t *filter) { int i; for (i=0; infilters; i++) { //if ( filter->filters[i].key ) free(filter->filters[i].key); free(filter->filters[i].str_value); free(filter->filters[i].tag); free(filter->filters[i].values); free(filter->filters[i].pass_samples); if (filter->filters[i].hash) khash_str2int_destroy_free(filter->filters[i].hash); if (filter->filters[i].regex) { regfree(filter->filters[i].regex); free(filter->filters[i].regex); } } free(filter->filters); free(filter->flt_stack); free(filter->str); free(filter->tmpi); free(filter); } int filter_test(filter_t *filter, bcf1_t *line, const uint8_t **samples) { bcf_unpack(line, filter->max_unpack); int i, nstack = 0; for (i=0; infilters; i++) { filter->filters[i].nsamples = 0; filter->filters[i].nvalues = 0; filter->filters[i].pass_site = -1; if ( filter->filters[i].tok_type == TOK_VAL ) { if ( filter->filters[i].setter ) // variable, query the VCF line filter->filters[i].setter(filter, line, &filter->filters[i]); else if ( filter->filters[i].key ) // string constant { filter->filters[i].str_value = filter->filters[i].key; filter->filters[i].values[0] = filter->filters[i].values[0]; filter->filters[i].nvalues = strlen(filter->filters[i].key); } else // numeric constant { filter->filters[i].values[0] = filter->filters[i].threshold; filter->filters[i].nvalues = 1; } filter->flt_stack[nstack++] = &filter->filters[i]; continue; } else if ( filter->filters[i].tok_type == TOK_FUNC ) // all functions take only one argument { filter->filters[i].setter(filter, line, filter->flt_stack[nstack-1]); continue; } if ( nstack<2 ) error("Error occurred while processing the filter \"%s\" (1:%d)\n", filter->str,nstack); // too few values left on the stack int is_str = filter->flt_stack[nstack-1]->is_str + filter->flt_stack[nstack-2]->is_str; if ( filter->filters[i].tok_type == TOK_OR || filter->filters[i].tok_type == TOK_OR_VEC ) { if ( filter->flt_stack[nstack-1]->pass_site<0 || filter->flt_stack[nstack-2]->pass_site<0 ) error("Error occurred while processing the filter \"%s\" (%d %d OR)\n", filter->str,filter->flt_stack[nstack-2]->pass_site,filter->flt_stack[nstack-1]->pass_site); filter->flt_stack[nstack-2]->pass_site = vector_logic_or(filter->flt_stack[nstack-2],filter->flt_stack[nstack-1], filter->filters[i].tok_type); nstack--; continue; } if ( filter->filters[i].tok_type == TOK_AND || filter->filters[i].tok_type == TOK_AND_VEC ) { if ( filter->flt_stack[nstack-1]->pass_site<0 || filter->flt_stack[nstack-2]->pass_site<0 ) error("Error occurred while processing the filter \"%s\" (%d %d AND)\n", filter->str,filter->flt_stack[nstack-2]->pass_site,filter->flt_stack[nstack-1]->pass_site); filter->flt_stack[nstack-2]->pass_site = vector_logic_and(filter->flt_stack[nstack-2],filter->flt_stack[nstack-1]); nstack--; continue; } if ( filter->filters[i].tok_type == TOK_ADD ) { VECTOR_ARITHMETICS(filter->flt_stack[nstack-2],filter->flt_stack[nstack-1],+); nstack--; continue; } else if ( filter->filters[i].tok_type == TOK_SUB ) { VECTOR_ARITHMETICS(filter->flt_stack[nstack-2],filter->flt_stack[nstack-1],-); nstack--; continue; } else if ( filter->filters[i].tok_type == TOK_MULT ) { VECTOR_ARITHMETICS(filter->flt_stack[nstack-2],filter->flt_stack[nstack-1],*); nstack--; continue; } else if ( filter->filters[i].tok_type == TOK_DIV ) { VECTOR_ARITHMETICS(filter->flt_stack[nstack-2],filter->flt_stack[nstack-1],/); nstack--; continue; } int is_true = 0; if ( !filter->flt_stack[nstack-1]->nvalues || !filter->flt_stack[nstack-2]->nvalues ) { filter->flt_stack[nstack-2]->nvalues = filter->flt_stack[nstack-2]->nsamples = 0; } else if ( filter->filters[i].tok_type == TOK_EQ ) { if ( filter->flt_stack[nstack-1]->comparator ) is_true = filter->flt_stack[nstack-1]->comparator(filter->flt_stack[nstack-1],filter->flt_stack[nstack-2],TOK_EQ,line); else if ( filter->flt_stack[nstack-2]->comparator ) is_true = filter->flt_stack[nstack-2]->comparator(filter->flt_stack[nstack-2],filter->flt_stack[nstack-1],TOK_EQ,line); else if ( is_str==2 ) // both are strings is_true = cmp_vector_strings(filter->flt_stack[nstack-2],filter->flt_stack[nstack-1],TOK_EQ); else if ( is_str==1 ) error("Comparing string to numeric value: %s\n", filter->str); else CMP_VECTORS(filter->flt_stack[nstack-2],filter->flt_stack[nstack-1],==,is_true); } else if ( filter->filters[i].tok_type == TOK_NE ) { if ( filter->flt_stack[nstack-1]->comparator ) is_true = filter->flt_stack[nstack-1]->comparator(filter->flt_stack[nstack-1],filter->flt_stack[nstack-2],TOK_NE,line); else if ( filter->flt_stack[nstack-2]->comparator ) is_true = filter->flt_stack[nstack-2]->comparator(filter->flt_stack[nstack-2],filter->flt_stack[nstack-1],TOK_NE,line); else if ( is_str==2 ) is_true = cmp_vector_strings(filter->flt_stack[nstack-2],filter->flt_stack[nstack-1],TOK_NE); else if ( is_str==1 ) error("Comparing string to numeric value: %s\n", filter->str); else CMP_VECTORS(filter->flt_stack[nstack-2],filter->flt_stack[nstack-1],!=,is_true); } else if ( filter->filters[i].tok_type == TOK_LIKE || filter->filters[i].tok_type == TOK_NLIKE ) { if ( is_str==2 ) { is_true = regex_vector_strings(filter->flt_stack[nstack-2],filter->flt_stack[nstack-1]); if ( filter->filters[i].tok_type == TOK_NLIKE ) is_true = is_true ? 0 : 1; } else error("The regex operator can be used on strings only: %s\n", filter->str); } else if ( is_str>0 ) error("Wrong operator in string comparison: %s [%s,%s]\n", filter->str, filter->flt_stack[nstack-1]->str_value, filter->flt_stack[nstack-2]->str_value); else if ( filter->filters[i].tok_type == TOK_LE ) CMP_VECTORS(filter->flt_stack[nstack-2],filter->flt_stack[nstack-1],<=,is_true) else if ( filter->filters[i].tok_type == TOK_LT ) CMP_VECTORS(filter->flt_stack[nstack-2],filter->flt_stack[nstack-1],<,is_true) else if ( filter->filters[i].tok_type == TOK_BT ) CMP_VECTORS(filter->flt_stack[nstack-2],filter->flt_stack[nstack-1],>,is_true) else if ( filter->filters[i].tok_type == TOK_BE ) CMP_VECTORS(filter->flt_stack[nstack-2],filter->flt_stack[nstack-1],>=,is_true) else error("FIXME: did not expect this .. tok_type %d = %d\n", i, filter->filters[i].tok_type); filter->flt_stack[nstack-2]->pass_site = is_true; nstack--; } if ( nstack>1 ) error("Error occurred while processing the filter \"%s\" (2:%d)\n", filter->str,nstack); // too few values left on the stack if ( samples ) { *samples = filter->max_unpack&BCF_UN_FMT ? filter->flt_stack[0]->pass_samples : NULL; if ( *samples && !filter->flt_stack[0]->nsamples ) { for (i=0; insamples; i++) filter->flt_stack[0]->pass_samples[i] = filter->flt_stack[0]->pass_site; } } return filter->flt_stack[0]->pass_site; } bcftools-1.2/filter.h000066400000000000000000000036251246371514100146370ustar00rootroot00000000000000/* filter.h -- filter expressions. Copyright (C) 2013-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #ifndef __FILTER_H__ #define __FILTER_H__ #include typedef struct _filter_t filter_t; /** * @hdr: BCF header file * @str: see the bcftools filter command help for description */ filter_t *filter_init(bcf_hdr_t *hdr, const char *str); void filter_destroy(filter_t *filter); /** * filter_test() - test whether the BCF record passes the test * @samples: if not NULL, a pointer to an array with samples statuses is * stored in the location referenced by @samples. The pointer * will be set to NULL if the FORMAT fields were not queried. * Returns 1 if the expression is true and 0 if false. */ int filter_test(filter_t *filter, bcf1_t *rec, const uint8_t **samples); void filter_expression_info(FILE *fp); #endif bcftools-1.2/gvcf.c000066400000000000000000000060231246371514100142650ustar00rootroot00000000000000/* gvcf.c -- support for gVCF files. Copyright (C) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include "call.h" void gvcf_write(htsFile *fh, gvcf_t *gvcf, bcf_hdr_t *hdr, bcf1_t *rec, int is_ref) { int i, ret, nsmpl = bcf_hdr_nsamples(hdr); // Flush gVCF block if chr changed, non-ref call encountered, depth is too // low, or no more records to come if ( rec && is_ref ) { bcf_unpack(rec, BCF_UN_ALL); // per-sample depth ret = bcf_get_format_int32(hdr, rec, "DP", &gvcf->dp, &gvcf->mdp); if ( ret==nsmpl ) { for (i=0; idp[i] < gvcf->min_dp ) break; if ( irid!=-1 && (!rec || gvcf->rid!=rec->rid || !is_ref || rec->pos > gvcf->end+1) ) { // mpileup can output two records with the same position, SNP and // indel. Make sure the end position does not include the non-variant // SNP position just before the indel. if ( rec && rec->rid==gvcf->rid && rec->pos==gvcf->end ) gvcf->end--; gvcf->end++; // from 0-based to 1-based coordinate bcf_clear1(gvcf->line); gvcf->line->rid = gvcf->rid; gvcf->line->pos = gvcf->start; gvcf->line->rlen = gvcf->end - gvcf->start; bcf_update_alleles_str(hdr, gvcf->line, gvcf->ref); bcf_update_info_int32(hdr, gvcf->line, "END", &gvcf->end, 1); bcf_update_genotypes(hdr, gvcf->line, gvcf->gt, nsmpl*2); bcf_write1(fh, hdr, gvcf->line); gvcf->rid = -1; } if ( !rec ) return; if ( is_ref ) { if ( gvcf->rid==-1 ) { gvcf->rid = rec->rid; gvcf->start = rec->pos; gvcf->ref[0] = rec->d.allele[0][0]; gvcf->ref[1] = 0; } gvcf->end = rec->pos; return; } bcf_write1(fh, hdr, rec); } bcftools-1.2/khash_str2str.h000066400000000000000000000052441246371514100161520ustar00rootroot00000000000000/* khash_str2str.h -- C-string to C-string hash table. Copyright (C) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #ifndef KHASH_STR2STR_H #define KHASH_STR2STR_H #include KHASH_MAP_INIT_STR(str2str, const char*) /* * Wrappers for khash dictionaries used by mpileup. */ static inline void *khash_str2str_init(void) { return kh_init(str2str); } /* * Destroy the hash structure, but not the keys */ static inline void khash_str2str_destroy(void *_hash) { khash_t(str2str) *hash = (khash_t(str2str)*)_hash; if (hash) kh_destroy(str2str, hash); // Note that strings are not freed. } /* * Destroys both the hash structure and the keys */ static inline void khash_str2str_destroy_free(void *_hash) { khash_t(str2str) *hash = (khash_t(str2str)*)_hash; khint_t k; if (hash == 0) return; for (k = 0; k < kh_end(hash); ++k) if (kh_exist(hash, k)) free((char*)kh_key(hash, k)); kh_destroy(str2str, hash); } /* * Returns value if key exists or NULL if not */ static inline char *khash_str2str_get(void *_hash, const char *str) { khash_t(str2str) *hash = (khash_t(str2str)*)_hash; khint_t k = kh_get(str2str, hash, str); if ( k == kh_end(hash) ) return NULL; return (char*)kh_val(hash, k); } /* * Set a new key,value pair. On success returns the bin index, on * error -1 is returned. */ static inline int khash_str2str_set(void *_hash, const char *str, const char *value) { khint_t k; int ret; khash_t(str2str) *hash = (khash_t(str2str)*)_hash; if ( !hash ) return -1; k = kh_put(str2str, hash, str, &ret); kh_val(hash,k) = value; return k; } #endif bcftools-1.2/kmin.c000066400000000000000000000161451246371514100143040ustar00rootroot00000000000000/* The MIT License Copyright (c) 2008, 2010 by Attractive Chaos Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ /* Hooke-Jeeves algorithm for nonlinear minimization Based on the pseudocodes by Bell and Pike (CACM 9(9):684-685), and the revision by Tomlin and Smith (CACM 12(11):637-638). Both of the papers are comments on Kaupe's Algorithm 178 "Direct Search" (ACM 6(6):313-314). The original algorithm was designed by Hooke and Jeeves (ACM 8:212-229). This program is further revised according to Johnson's implementation at Netlib (opt/hooke.c). Hooke-Jeeves algorithm is very simple and it works quite well on a few examples. However, it might fail to converge due to its heuristic nature. A possible improvement, as is suggested by Johnson, may be to choose a small r at the beginning to quickly approach to the minimum and a large r at later step to hit the minimum. */ #include #include #include #include "kmin.h" static double __kmin_hj_aux(kmin_f func, int n, double *x1, void *data, double fx1, double *dx, int *n_calls) { int k, j = *n_calls; double ftmp; for (k = 0; k != n; ++k) { x1[k] += dx[k]; ftmp = func(n, x1, data); ++j; if (ftmp < fx1) fx1 = ftmp; else { /* search the opposite direction */ dx[k] = 0.0 - dx[k]; x1[k] += dx[k] + dx[k]; ftmp = func(n, x1, data); ++j; if (ftmp < fx1) fx1 = ftmp; else x1[k] -= dx[k]; /* back to the original x[k] */ } } *n_calls = j; return fx1; /* here: fx1=f(n,x1) */ } double kmin_hj(kmin_f func, int n, double *x, void *data, double r, double eps, int max_calls) { double fx, fx1, *x1, *dx, radius; int k, n_calls = 0; x1 = (double*)calloc(n, sizeof(double)); dx = (double*)calloc(n, sizeof(double)); for (k = 0; k != n; ++k) { /* initial directions, based on MGJ */ dx[k] = fabs(x[k]) * r; if (dx[k] == 0) dx[k] = r; } radius = r; fx1 = fx = func(n, x, data); ++n_calls; for (;;) { memcpy(x1, x, n * sizeof(double)); /* x1 = x */ fx1 = __kmin_hj_aux(func, n, x1, data, fx, dx, &n_calls); while (fx1 < fx) { for (k = 0; k != n; ++k) { double t = x[k]; dx[k] = x1[k] > x[k]? fabs(dx[k]) : 0.0 - fabs(dx[k]); x[k] = x1[k]; x1[k] = x1[k] + x1[k] - t; } fx = fx1; if (n_calls >= max_calls) break; fx1 = func(n, x1, data); ++n_calls; fx1 = __kmin_hj_aux(func, n, x1, data, fx1, dx, &n_calls); if (fx1 >= fx) break; for (k = 0; k != n; ++k) if (fabs(x1[k] - x[k]) > .5 * fabs(dx[k])) break; if (k == n) break; } if (radius >= eps) { if (n_calls >= max_calls) break; radius *= r; for (k = 0; k != n; ++k) dx[k] *= r; } else break; /* converge */ } free(x1); free(dx); return fx1; } // I copied this function somewhere several years ago with some of my modifications, but I forgot the source. double kmin_brent(kmin1_f func, double a, double b, void *data, double tol, double *xmin) { double bound, u, r, q, fu, tmp, fa, fb, fc, c; const double gold1 = 1.6180339887; const double gold2 = 0.3819660113; const double tiny = 1e-20; const int max_iter = 100; double e, d, w, v, mid, tol1, tol2, p, eold, fv, fw; int iter; fa = func(a, data); fb = func(b, data); if (fb > fa) { // swap, such that f(a) > f(b) tmp = a; a = b; b = tmp; tmp = fa; fa = fb; fb = tmp; } c = b + gold1 * (b - a), fc = func(c, data); // golden section extrapolation while (fb > fc) { bound = b + 100.0 * (c - b); // the farthest point where we want to go r = (b - a) * (fb - fc); q = (b - c) * (fb - fa); if (fabs(q - r) < tiny) { // avoid 0 denominator tmp = q > r? tiny : 0.0 - tiny; } else tmp = q - r; u = b - ((b - c) * q - (b - a) * r) / (2.0 * tmp); // u is the parabolic extrapolation point if ((b > u && u > c) || (b < u && u < c)) { // u lies between b and c fu = func(u, data); if (fu < fc) { // (b,u,c) bracket the minimum a = b; b = u; fa = fb; fb = fu; break; } else if (fu > fb) { // (a,b,u) bracket the minimum c = u; fc = fu; break; } u = c + gold1 * (c - b); fu = func(u, data); // golden section extrapolation } else if ((c > u && u > bound) || (c < u && u < bound)) { // u lies between c and bound fu = func(u, data); if (fu < fc) { // fb > fc > fu b = c; c = u; u = c + gold1 * (c - b); fb = fc; fc = fu; fu = func(u, data); } else { // (b,c,u) bracket the minimum a = b; b = c; c = u; fa = fb; fb = fc; fc = fu; break; } } else if ((u > bound && bound > c) || (u < bound && bound < c)) { // u goes beyond the bound u = bound; fu = func(u, data); } else { // u goes the other way around, use golden section extrapolation u = c + gold1 * (c - b); fu = func(u, data); } a = b; b = c; c = u; fa = fb; fb = fc; fc = fu; } if (a > c) u = a, a = c, c = u; // swap // now, afb and fb tol1) { // related to parabolic interpolation r = (b - w) * (fb - fv); q = (b - v) * (fb - fw); p = (b - v) * q - (b - w) * r; q = 2.0 * (q - r); if (q > 0.0) p = 0.0 - p; else q = 0.0 - q; eold = e; e = d; if (fabs(p) >= fabs(0.5 * q * eold) || p <= q * (a - b) || p >= q * (c - b)) { d = gold2 * (e = (b >= mid ? a - b : c - b)); } else { d = p / q; u = b + d; // actual parabolic interpolation happens here if (u - a < tol2 || c - u < tol2) d = (mid > b)? tol1 : 0.0 - tol1; } } else d = gold2 * (e = (b >= mid ? a - b : c - b)); // golden section interpolation u = fabs(d) >= tol1 ? b + d : b + (d > 0.0? tol1 : -tol1); fu = func(u, data); if (fu <= fb) { // u is the minimum point so far if (u >= b) a = b; else c = b; v = w; w = b; b = u; fv = fw; fw = fb; fb = fu; } else { // adjust (a,c) and (u,v,w) if (u < b) a = u; else c = u; if (fu <= fw || w == b) { v = w; w = u; fv = fw; fw = fu; } else if (fu <= fv || v == b || v == w) { v = u; fv = fu; } } } *xmin = b; return fb; } bcftools-1.2/kmin.h000066400000000000000000000031231246371514100143010ustar00rootroot00000000000000/* Copyright (c) 2008, 2010 by Attractive Chaos Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #ifndef KMIN_H #define KMIN_H #define KMIN_RADIUS 0.5 #define KMIN_EPS 1e-7 #define KMIN_MAXCALL 50000 typedef double (*kmin_f)(int, double*, void*); typedef double (*kmin1_f)(double, void*); #ifdef __cplusplus extern "C" { #endif double kmin_hj(kmin_f func, int n, double *x, void *data, double r, double eps, int max_calls); double kmin_brent(kmin1_f func, double a, double b, void *data, double tol, double *xmin); #ifdef __cplusplus } #endif #endif bcftools-1.2/main.c000066400000000000000000000201201246371514100142560ustar00rootroot00000000000000/* main.c -- main bcftools command front-end. Copyright (C) 2012-2015 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include "version.h" #include "bcftools.h" int main_tabix(int argc, char *argv[]); int main_vcfindex(int argc, char *argv[]); int main_vcfstats(int argc, char *argv[]); int main_vcfisec(int argc, char *argv[]); int main_vcfmerge(int argc, char *argv[]); int main_vcfquery(int argc, char *argv[]); int main_vcffilter(int argc, char *argv[]); int main_vcfsom(int argc, char *argv[]); int main_vcfnorm(int argc, char *argv[]); int main_vcfgtcheck(int argc, char *argv[]); int main_vcfview(int argc, char *argv[]); int main_vcfcall(int argc, char *argv[]); int main_vcfannotate(int argc, char *argv[]); int main_vcfroh(int argc, char *argv[]); int main_vcfconcat(int argc, char *argv[]); int main_reheader(int argc, char *argv[]); int main_vcfconvert(int argc, char *argv[]); int main_vcfcnv(int argc, char *argv[]); #if USE_GPL int main_polysomy(int argc, char *argv[]); #endif int main_plugin(int argc, char *argv[]); int main_consensus(int argc, char *argv[]); typedef struct { int (*func)(int, char*[]); const char *alias, *help; } cmd_t; static cmd_t cmds[] = { { .func = NULL, .alias = "Indexing", .help = NULL }, { .func = main_vcfindex, .alias = "index", .help = "index VCF/BCF files" }, { .func = main_tabix, .alias = "tabix", .help = "-tabix for BGZF'd BED, GFF, SAM, VCF and more" // do not advertise; only keep here for testing }, { .func = NULL, .alias = "VCF/BCF manipulation", .help = NULL }, { .func = main_vcfannotate, .alias = "annotate", .help = "annotate and edit VCF/BCF files", }, { .func = main_vcfconcat, .alias = "concat", .help = "concatenate VCF/BCF files from the same set of samples" }, { .func = main_vcfconvert, .alias = "convert", .help = "convert VCF/BCF files to different formats and back" }, { .func = main_vcfisec, .alias = "isec", .help = "intersections of VCF/BCF files" }, { .func = main_vcfmerge, .alias = "merge", .help = "merge VCF/BCF files files from non-overlapping sample sets" }, { .func = main_vcfnorm, .alias = "norm", .help = "left-align and normalize indels" }, { .func = main_plugin, .alias = "plugin", .help = "user-defined plugins" }, { .func = main_vcfquery, .alias = "query", .help = "transform VCF/BCF into user-defined formats" }, { .func = main_reheader, .alias = "reheader", .help = "modify VCF/BCF header, change sample names" }, { .func = main_vcfview, .alias = "view", .help = "VCF/BCF conversion, view, subset and filter VCF/BCF files" }, { .func = NULL, .alias = "VCF/BCF analysis", .help = NULL }, { .func = main_vcfcall, .alias = "call", .help = "SNP/indel calling" }, { .func = main_consensus, .alias = "consensus", .help = "create consensus sequence by applying VCF variants" }, { .func = main_vcfcnv, .alias = "cnv", .help = "-HMM CNV calling" // do not advertise yet }, { .func = main_vcffilter, .alias = "filter", .help = "filter VCF/BCF files using fixed thresholds" }, { .func = main_vcfgtcheck, .alias = "gtcheck", .help = "check sample concordance, detect sample swaps and contamination" }, #if USE_GPL { .func = main_polysomy, .alias = "polysomy", .help = "-detect number of chromosomal copies", }, #endif { .func = main_vcfroh, .alias = "roh", .help = "identify runs of autozygosity (HMM)", }, { .func = main_vcfstats, .alias = "stats", .help = "produce VCF/BCF stats" }, { .func = main_vcfsom, .alias = "som", .help = "-filter using Self-Organized Maps (experimental)" // do not advertise }, { .func = NULL, .alias = NULL, .help = NULL } }; char *bcftools_version(void) { return BCFTOOLS_VERSION; } static void usage(FILE *fp) { fprintf(fp, "\n"); fprintf(fp, "Program: bcftools (Tools for variant calling and manipulating VCFs and BCFs)\n"); #if USE_GPL fprintf(fp, "License: GNU GPLv3+, due to use of the GNU Scientific Library\n"); #endif fprintf(fp, "Version: %s (using htslib %s)\n", bcftools_version(), hts_version()); fprintf(fp, "\n"); fprintf(fp, "Usage: bcftools \n"); fprintf(fp, "\n"); fprintf(fp, "Commands:\n"); int i = 0; const char *sep = NULL; while (cmds[i].alias) { if ( !cmds[i].func ) sep = cmds[i].alias; if ( sep ) { fprintf(fp, "\n -- %s\n", sep); sep = NULL; } if ( cmds[i].func && cmds[i].help[0]!='-' ) fprintf(fp, " %-12s %s\n", cmds[i].alias, cmds[i].help); i++; } fprintf(fp,"\n"); fprintf(fp, " Most commands accept VCF, bgzipped VCF, and BCF with the file type detected\n" " automatically even when streaming from a pipe. Indexed VCF and BCF will work\n" " in all situations. Un-indexed VCF and BCF and streams will work in most but\n" " not all situations.\n"); fprintf(fp,"\n"); } int main(int argc, char *argv[]) { if (argc < 2) { usage(stderr); return 1; } if (strcmp(argv[1], "version") == 0 || strcmp(argv[1], "--version") == 0 || strcmp(argv[1], "-v") == 0) { printf("bcftools %s\nUsing htslib %s\nCopyright (C) 2015 Genome Research Ltd.\n", bcftools_version(), hts_version()); #if USE_GPL printf("License GPLv3+: GNU GPL version 3 or later \n"); #else printf("License Expat: The MIT/Expat license\n"); #endif printf("This is free software: you are free to change and redistribute it.\nThere is NO WARRANTY, to the extent permitted by law.\n"); return 0; } else if (strcmp(argv[1], "--version-only") == 0) { printf("%s+htslib-%s\n", bcftools_version(), hts_version()); return 0; } else if (strcmp(argv[1], "help") == 0 || strcmp(argv[1], "--help") == 0 || strcmp(argv[1], "-h") == 0) { if (argc == 2) { usage(stdout); return 0; } // Otherwise change "bcftools help COMMAND [...]" to "bcftools COMMAND"; // main_xyz() functions by convention display the subcommand's usage // when invoked without any arguments. argv++; argc = 2; } else if ( argv[1][0]=='+' ) { // "bcftools plugin name" can be run as "bcftools +name" argv[1]++; argv[0] = "plugin"; argv--; argc++; } int i = 0; while (cmds[i].alias) { if (cmds[i].func && strcmp(argv[1],cmds[i].alias)==0) { return cmds[i].func(argc-1,argv+1); } i++; } fprintf(stderr, "[E::%s] unrecognized command '%s'\n", __func__, argv[1]); return 1; } bcftools-1.2/mcall.c000066400000000000000000001550271246371514100144410ustar00rootroot00000000000000/* mcall.c -- multiallelic and rare variant calling. Copyright (C) 2012-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include "call.h" // Using priors for GTs does not seem to be mathematically justified. Although // it seems effective in removing false calls, it also flips a significant // proportion of HET genotypes. Better is to filter by FORMAT/GQ using // `bcftools filter`. #define USE_PRIOR_FOR_GTS 0 // Go with uniform PLs for samples with no coverage. If unset, missing // genotypes is reported instead. #define FLAT_PDG_FOR_MISSING 0 // Estimate QS (combined quality and allele frequencies) from PLs #define QS_FROM_PDG 0 void qcall_init(call_t *call) { return; } void qcall_destroy(call_t *call) { return; } int qcall(call_t *call, bcf1_t *rec) { // QCall format: // chromosome, position, reference allele, depth, mapping quality, 0, .. error("TODO: qcall output\n"); return 0; } void call_init_pl2p(call_t *call) { int i; for (i=0; i<256; i++) call->pl2p[i] = pow(10., -i/10.); } // Macros for accessing call->trio and call->ntrio #define FTYPE_222 0 // family type: all diploid #define FTYPE_121 1 // chrX, the child is a boy #define FTYPE_122 2 // chrX, a girl #define FTYPE_101 3 // chrY, boy #define FTYPE_100 4 // chrY, girl #define GT_SKIP 0xf // empty genotype (chrY in females) #define IS_POW2(x) (!((x) & ((x) - 1))) // zero is permitted #define IS_HOM(x) IS_POW2(x) // Pkij = P(k|i,j) tells how likely it is to be a het if the parents // are homs etc. The consistency of i,j,k has been already checked. // Parameters are alleles and ploidy of father, mother, kid // Returns 2/Pkij. int calc_Pkij(int fals, int mals, int kals, int fpl, int mpl, int kpl) { int als = fals|mals|kals; if ( IS_HOM(als) ) return 2; // all are the same: child must be a HOM, P=1 if ( fpl==1 ) { if ( kpl==1 ) // chr X, the child is a boy, the copy is inherited from the mother { if ( IS_HOM(mals) ) return 2; // 0 11 -> P(1) = 1 return 4; // 0 01 -> P(0) = P(1) = 1/2 } // chr X, the child is a girl if ( IS_HOM(mals) ) return 2; // 0 11 -> P(01) = 1 return 4; // 0 01 -> P(00) = P(01) = 1/2 } if ( IS_HOM(fals) && IS_HOM(mals) ) return 2; // 00 11 01, the child must be a HET, P=1 if ( !IS_HOM(fals) && !IS_HOM(mals) ) { if ( IS_HOM(kals) ) return 8; // 01 01 00 or 01 01 11, P(k=HOM) = 1/4 return 4; // 01 01 01, P(k=HET) = 1/2 } return 4; // 00 01, P(k=HET) = P(k=HOM) = 1/2 } // Initialize ntrio and trio: ntrio lists the number of possible // genotypes given combination of haploid/diploid genomes and the // number of alleles. trio lists allowed genotype combinations: // 4bit: 2/Pkij, 4: father, 4: mother, 4: child // See also mcall_call_trio_genotypes() // static void mcall_init_trios(call_t *call) { // 23, 138, 478 possible diploid trio genotypes with 2, 3, 4 alleles call->ntrio[FTYPE_222][2] = 15; call->ntrio[FTYPE_222][3] = 78; call->ntrio[FTYPE_222][4] = 250; call->ntrio[FTYPE_121][2] = 8; call->ntrio[FTYPE_121][3] = 27; call->ntrio[FTYPE_121][4] = 64; call->ntrio[FTYPE_122][2] = 8; call->ntrio[FTYPE_122][3] = 27; call->ntrio[FTYPE_122][4] = 64; call->ntrio[FTYPE_101][2] = 2; call->ntrio[FTYPE_101][3] = 3; call->ntrio[FTYPE_101][4] = 4; call->ntrio[FTYPE_100][2] = 2; call->ntrio[FTYPE_100][3] = 3; call->ntrio[FTYPE_100][4] = 4; int nals, itype; for (itype=0; itype<=4; itype++) { for (nals=2; nals<=4; nals++) call->trio[itype][nals] = (uint16_t*) malloc(sizeof(uint16_t)*call->ntrio[itype][nals]); } // max 10 possible diploid genotypes int gts[10]; for (nals=2; nals<=4; nals++) { int i,j,k, n = 0, ngts = 0; for (i=0; itrio[FTYPE_222][nals][n++] = Pkij<<12 | i<<8 | j<<4 | k; // father, mother, child } assert( n==call->ntrio[FTYPE_222][nals] ); // 121: chrX, boy n = 0; for (i=0; itrio[FTYPE_121][nals][n++] = Pkij<<12 | i<<8 | j<<4 | k; } assert( n==call->ntrio[FTYPE_121][nals] ); // 122: chrX, girl n = 0; for (i=0; itrio[FTYPE_122][nals][n++] = Pkij<<12 | i<<8 | j<<4 | k; } assert( n==call->ntrio[FTYPE_122][nals] ); // 101: chrY, boy n = 0; for (i=0; itrio[FTYPE_101][nals][n++] = 1<<12 | i<<8 | GT_SKIP<<4 | k; } assert( n==call->ntrio[FTYPE_101][nals] ); // 100: chrY, girl n = 0; for (i=0; itrio[FTYPE_100][nals][n++] = 1<<12 | i<<8 | GT_SKIP<<4 | GT_SKIP; } assert( n==call->ntrio[FTYPE_100][nals] ); } call->GLs = (double*) calloc(bcf_hdr_nsamples(call->hdr)*10,sizeof(double)); int i, j; for (i=0; infams; i++) { family_t *fam = &call->fams[i]; int ploidy[3]; for (j=0; j<3; j++) ploidy[j] = call->ploidy[fam->sample[j]]; if ( ploidy[FATHER]==2 ) // not X, not Y { if ( ploidy[MOTHER]!=2 || ploidy[CHILD]!=2 ) error("Incorrect ploidy: %d %d %d\n", ploidy[FATHER],ploidy[MOTHER],ploidy[CHILD]); fam->type = FTYPE_222; continue; } if ( ploidy[FATHER]!=1 || ploidy[MOTHER]==1 ) error("Incorrect ploidy: %d %d %d\n", ploidy[FATHER],ploidy[MOTHER],ploidy[CHILD]); if ( ploidy[MOTHER]==2 ) // X { if ( ploidy[CHILD]==0 ) error("Incorrect ploidy: %d %d %d\n", ploidy[FATHER],ploidy[MOTHER],ploidy[CHILD]); fam->type = ploidy[CHILD]==2 ? FTYPE_122 : FTYPE_121; // a girl or a boy } else // Y { if ( ploidy[CHILD]==2 ) error("Incorrect ploidy: %d %d %d\n", ploidy[FATHER],ploidy[MOTHER],ploidy[CHILD]); fam->type = ploidy[CHILD]==0 ? FTYPE_100 : FTYPE_101; // a girl or a boy } } } static void mcall_destroy_trios(call_t *call) { int i, j; for (i=2; i<=4; i++) for (j=0; j<=4; j++) free(call->trio[j][i]); } void mcall_init(call_t *call) { call_init_pl2p(call); call->nqsum = 5; call->qsum = (float*) malloc(sizeof(float)*call->nqsum); // will be expanded later if ncessary call->nals_map = 5; call->als_map = (int*) malloc(sizeof(int)*call->nals_map); call->npl_map = 5*(5+1)/2; // will be expanded later if necessary call->pl_map = (int*) malloc(sizeof(int)*call->npl_map); call->gts = (int32_t*) calloc(bcf_hdr_nsamples(call->hdr)*2,sizeof(int32_t)); // assuming at most diploid everywhere if ( call->flag & CALL_CONSTR_TRIO ) { call->cgts = (int32_t*) calloc(bcf_hdr_nsamples(call->hdr),sizeof(int32_t)); call->ugts = (int32_t*) calloc(bcf_hdr_nsamples(call->hdr),sizeof(int32_t)); mcall_init_trios(call); bcf_hdr_append(call->hdr,"##FORMAT="); bcf_hdr_append(call->hdr,"##FORMAT="); } if ( call->flag & CALL_CONSTR_ALLELES ) call->vcmp = vcmp_init(); bcf_hdr_append(call->hdr,"##FORMAT="); if ( call->output_tags & CALL_FMT_GQ ) bcf_hdr_append(call->hdr,"##FORMAT="); if ( call->output_tags & CALL_FMT_GP ) bcf_hdr_append(call->hdr,"##FORMAT="); if ( call->output_tags & (CALL_FMT_GQ|CALL_FMT_GP) ) call->GQs = (int32_t*) malloc(sizeof(int32_t)*bcf_hdr_nsamples(call->hdr)); bcf_hdr_append(call->hdr,"##INFO="); bcf_hdr_append(call->hdr,"##INFO="); bcf_hdr_append(call->hdr,"##INFO="); bcf_hdr_append(call->hdr,"##INFO="); bcf_hdr_append(call->hdr,"##INFO="); bcf_hdr_append(call->hdr,"##INFO="); // init the prior if ( call->theta>0 ) { int i, n = 0; if ( !call->ploidy ) n = 2*bcf_hdr_nsamples(call->hdr); // all are diploid else { for (i=0; ihdr); i++) n += call->ploidy[i]; } // Watterson factor, here aM_1 = aM_2 = 1 double aM = 1; for (i=2; itheta *= aM; if ( call->theta >= 1 ) { fprintf(stderr,"The prior is too big (theta*aM=%.2f), going with 0.99\n", call->theta); call->theta = 0.99; } call->theta = log(call->theta); } return; } void mcall_destroy(call_t *call) { if (call->vcmp) vcmp_destroy(call->vcmp); free(call->itmp); mcall_destroy_trios(call); free(call->GPs); free(call->GLs); free(call->GQs); free(call->anno16); free(call->PLs); free(call->qsum); free(call->als_map); free(call->pl_map); free(call->gts); free(call->cgts); free(call->ugts); free(call->pdg); free(call->als); return; } // Inits P(D|G): convert PLs from log space and normalize. In case of zero // depth, missing PLs are all zero. In this case, pdg's are set to 0 // so that the corresponding genotypes can be set as missing and the // qual calculation is not affected. // Missing values are replaced by generic likelihoods when X (unseen allele) is // present. // NB: While the -m callig model uses the pdgs in canonical order, // the original samtools -c calling code uses pdgs in reverse order (AA comes // first, RR last). // NB: Ploidy is not taken into account here, which is incorrect. void set_pdg(double *pl2p, int *PLs, double *pdg, int n_smpl, int n_gt, int unseen) { int i, j, nals; // find out the number of alleles, expecting diploid genotype likelihoods bcf_gt2alleles(n_gt-1, &i, &nals); assert( i==nals ); nals++; for (i=0; ipdg; int ngts = rec->n_allele*(rec->n_allele+1)/2; int i,nsmpl = bcf_hdr_nsamples(call->hdr); hts_expand(float,rec->n_allele,call->nqsum,call->qsum); for (i=0; in_allele; i++) call->qsum[i] = 0; for (i=0; in_allele; a++) { for (b=0; b<=a; b++) { call->qsum[a] += pdg[k]; call->qsum[b] += pdg[k]; k++; } } pdg += ngts; } float sum = 0; for (i=0; in_allele; i++) sum += call->qsum[i]; if ( sum ) for (i=0; in_allele; i++) call->qsum[i] /= sum; } // Create mapping between old and new (trimmed) alleles void init_allele_trimming_maps(call_t *call, int als, int nals) { int i, j; // als_map: old(i) -> new(j) for (i=0, j=0; ials_map[i] = j++; else call->als_map[i] = -1; } if ( !call->pl_map ) return; // pl_map: new(k) -> old(l) int k = 0, l = 0; for (i=0; ipl_map[k++] = l; l++; } } } double binom_dist(int N, double p, int k) { int mean = (int) (N*p); if ( mean==k ) return 1.0; double log_p = (k-mean)*log(p) + (mean-k)*log(1.0-p); if ( k > N - k ) k = N - k; if ( mean > N - mean ) mean = N - mean; if ( k < mean ) { int tmp = k; k = mean; mean = tmp; } double diff = k - mean; double val = 1.0; int i; for (i=0; i10 && (1-q)*ndiploid>10 ) || ndiploid>200 ) { //fprintf(stderr,"out: mean=%e p=%e\n", mean,exp(-0.5*(nhets-mean)*(nhets-mean)/(mean*(1-q)))); return exp(-0.5*(nhets-mean)*(nhets-mean)/(mean*(1-q))); } return binom_dist(ndiploid, q, nhets); } float calc_HOB(int nref, int nalt, int nhets, int ndiploid) { if ( !nref || !nalt || !ndiploid ) return HUGE_VAL; double fref = (double)nref/(nref+nalt); // fraction of reference allelels double falt = (double)nalt/(nref+nalt); // non-ref als return fabs((double)nhets/ndiploid - 2*fref*falt); } /** * log(sum_i exp(a_i)) */ static inline double logsumexp(double *vals, int nvals) { int i; double max_exp = vals[0]; for (i=1; ib ) return log(1 + exp(b-a)) + a; else return log(1 + exp(a-b)) + b; } // Macro to set the most likely alleles #define UPDATE_MAX_LKs(als) { \ if ( max_lkhdr); int ngts = nals*(nals+1)/2; // Single allele for (ia=0; iapdg + iaa; for (isample=0; isampletheta; // the prior UPDATE_MAX_LKs(1<1 ) { for (ia=0; iaqsum[ia]==0 ) continue; int iaa = (ia+1)*(ia+2)/2-1; for (ib=0; ibqsum[ib]==0 ) continue; double lk_tot = 0; int lk_tot_set = 0; double fa = call->qsum[ia]/(call->qsum[ia]+call->qsum[ib]); double fb = call->qsum[ib]/(call->qsum[ia]+call->qsum[ib]); double fab = 2*fa*fb; fa *= fa; fb *= fb; int isample, ibb = (ib+1)*(ib+2)/2-1, iab = iaa - ia + ib; double *pdg = call->pdg; for (isample=0; isampleploidy || call->ploidy[isample]==2 ) val = fa*pdg[iaa] + fb*pdg[ibb] + fab*pdg[iab]; else if ( call->ploidy && call->ploidy[isample]==1 ) val = fa*pdg[iaa] + fb*pdg[ibb]; if ( val ) { lk_tot += log(val); lk_tot_set = 1; } pdg += ngts; } if ( ia!=0 ) lk_tot += call->theta; // the prior if ( ib!=0 ) lk_tot += call->theta; UPDATE_MAX_LKs(1<2 ) { for (ia=0; iaqsum[ia]==0 ) continue; int iaa = (ia+1)*(ia+2)/2-1; for (ib=0; ibqsum[ib]==0 ) continue; int ibb = (ib+1)*(ib+2)/2-1; int iab = iaa - ia + ib; for (ic=0; icqsum[ic]==0 ) continue; double lk_tot = 0; int lk_tot_set = 1; double fa = call->qsum[ia]/(call->qsum[ia]+call->qsum[ib]+call->qsum[ic]); double fb = call->qsum[ib]/(call->qsum[ia]+call->qsum[ib]+call->qsum[ic]); double fc = call->qsum[ic]/(call->qsum[ia]+call->qsum[ib]+call->qsum[ic]); double fab = 2*fa*fb, fac = 2*fa*fc, fbc = 2*fb*fc; fa *= fa; fb *= fb; fc *= fc; int isample, icc = (ic+1)*(ic+2)/2-1; int iac = iaa - ia + ic, ibc = ibb - ib + ic; double *pdg = call->pdg; for (isample=0; isampleploidy || call->ploidy[isample]==2 ) val = fa*pdg[iaa] + fb*pdg[ibb] + fc*pdg[icc] + fab*pdg[iab] + fac*pdg[iac] + fbc*pdg[ibc]; else if ( call->ploidy && call->ploidy[isample]==1 ) val = fa*pdg[iaa] + fb*pdg[ibb] + fc*pdg[icc]; if ( val ) { lk_tot += log(val); lk_tot_set = 1; } pdg += ngts; } if ( ia!=0 ) lk_tot += call->theta; // the prior if ( ib!=0 ) lk_tot += call->theta; // the prior if ( ic!=0 ) lk_tot += call->theta; // the prior UPDATE_MAX_LKs(1<ref_lk = ref_lk; call->lk_sum = lk_sum; *out_als = max_als; int i, n = 0; for (i=0; ihdr); for (i=0; i<4; i++) call->ac[i] = 0; call->nhets = 0; call->ndiploid = 0; // Set all genotypes to 0/0 or 0 int *gts = call->gts; double *pdg = call->pdg; int isample; for (isample = 0; isample < nsmpl; isample++) { int ploidy = call->ploidy ? call->ploidy[isample] : 2; for (i=0; iac[0] += ploidy; } gts += 2; pdg += ngts; } } static void mcall_call_genotypes(call_t *call, bcf1_t *rec, int nals, int nout_als, int out_als) { int ia, ib, i; int ngts = nals*(nals+1)/2; int nsmpl = bcf_hdr_nsamples(call->hdr); int nout_gts = nout_als*(nout_als+1)/2; hts_expand(float,nout_gts*nsmpl,call->nGPs,call->GPs); for (i=0; i<4; i++) call->ac[i] = 0; call->nhets = 0; call->ndiploid = 0; #if USE_PRIOR_FOR_GTS float prior = exp(call->theta); #endif float *gps = call->GPs - nout_gts; double *pdg = call->pdg - ngts; int *gts = call->gts - 2; int isample; for (isample = 0; isample < nsmpl; isample++) { int ploidy = call->ploidy ? call->ploidy[isample] : 2; assert( ploidy>=0 && ploidy<=2 ); pdg += ngts; gts += 2; gps += nout_gts; if ( !ploidy ) { gts[0] = bcf_gt_missing; gts[1] = bcf_int32_vector_end; gps[0] = -1; continue; } #if !FLAT_PDG_FOR_MISSING // Skip samples with zero depth, they have all pdg's equal to 0 for (i=0; indiploid++; // Default fallback for the case all LKs are the same gts[0] = bcf_gt_unphased(0); gts[1] = ploidy==2 ? bcf_gt_unphased(0) : bcf_int32_vector_end; // Non-zero depth, determine the most likely genotype double best_lk = 0; for (ia=0; iaqsum[ia]*call->qsum[ia]; #if USE_PRIOR_FOR_GTS if ( ia!=0 ) lk *= prior; #endif int igt = ploidy==2 ? bcf_alleles2gt(call->als_map[ia],call->als_map[ia]) : call->als_map[ia]; gps[igt] = lk; if ( best_lk < lk ) { best_lk = lk; gts[0] = bcf_gt_unphased(call->als_map[ia]); } } if ( ploidy==2 ) { gts[1] = gts[0]; for (ia=0; iaqsum[ia]*call->qsum[ib]; #if USE_PRIOR_FOR_GTS if ( ia!=0 ) lk *= prior; if ( ib!=0 ) lk *= prior; #endif int igt = bcf_alleles2gt(call->als_map[ia],call->als_map[ib]); gps[igt] = lk; if ( best_lk < lk ) { best_lk = lk; gts[0] = bcf_gt_unphased(call->als_map[ib]); gts[1] = bcf_gt_unphased(call->als_map[ia]); } } } if ( gts[0] != gts[1] ) call->nhets++; } else gts[1] = bcf_int32_vector_end; call->ac[ bcf_gt_allele(gts[0]) ]++; if ( gts[1]!=bcf_int32_vector_end ) call->ac[ bcf_gt_allele(gts[1]) ]++; } if ( call->output_tags & (CALL_FMT_GQ|CALL_FMT_GP) ) { double max, sum; for (isample=0; isampleGPs + isample*nout_gts; int nmax; if ( call->ploidy ) { if ( call->ploidy[isample]==2 ) nmax = nout_gts; else if ( call->ploidy[isample]==1 ) nmax = nout_als; else nmax = 0; } else nmax = nout_gts; max = gps[0]; if ( max<0 || nmax==0 ) { // no call if ( call->output_tags & CALL_FMT_GP ) { for (i=0; iGQs[isample] = 0; continue; } sum = gps[0]; for (i=1; iGQs[isample] = max<=INT8_MAX ? max : INT8_MAX; if ( call->output_tags & CALL_FMT_GP ) { assert( max ); for (i=0; ioutput_tags & CALL_FMT_GP ) bcf_update_format_float(call->hdr, rec, "GP", call->GPs, nsmpl*nout_gts); if ( call->output_tags & CALL_FMT_GQ ) bcf_update_format_int32(call->hdr, rec, "GQ", call->GQs, nsmpl); } /** Pm = P(mendelian) .. parameter to vary, 1-Pm is the probability of novel mutation. When trio_Pm_ins is negative, Pm is calculated dynamically according to indel length. For simplicity, only the first ALT is considered. Pkij = P(k|i,j) .. probability that the genotype combination i,j,k is consistent with mendelian inheritance (the likelihood that offspring of two HETs is a HOM is smaller than it being a HET) P_uc(F=i,M=j,K=k) = P(F=i) . P(M=j) . P(K=k) .. unconstrained P P_c(F=i,M=j,K=k) = P_uc . Pkij .. constrained P P(F=i,M=j,K=k) = P_uc . (1 - Pm) + P_c . Pm = P_uc . [1 - Pm + Pkij . Pm] We choose genotype combination i,j,k which maximizes P(F=i,M=j,K=k). This probability gives the quality GQ(Trio). Individual qualities are calculated as GQ(F=i,M=j,K=k) = P(F=i,M=j,K=k) / \sum_{x,y} P(F=i,M=x,K=y) */ static void mcall_call_trio_genotypes(call_t *call, bcf1_t *rec, int nals, int nout_als, int out_als) { int ia, ib, i; int nsmpl = bcf_hdr_nsamples(call->hdr); int ngts = nals*(nals+1)/2; double *gls = call->GLs - ngts; double *pdg = call->pdg - ngts; // Calculate individuals' genotype likelihoods P(X=i) int isample; for (isample = 0; isample < nsmpl; isample++) { int ploidy = call->ploidy ? call->ploidy[isample] : 2; int32_t *gts = call->ugts + isample; gls += ngts; pdg += ngts; // Skip samples with all pdg's equal to 1. These have zero depth. for (i=0; ials_map[ia],call->als_map[ia]); double lk = pdg[iaa]*call->qsum[ia]*call->qsum[ia]; sum_lk += lk; gls[idx] = lk; if ( best_lk < lk ) { best_lk = lk; gts[0] = bcf_alleles2gt(call->als_map[ia],call->als_map[ia]); } } if ( ploidy==2 ) { for (ia=0; iaals_map[ia],call->als_map[ib]); double lk = 2*pdg[iab]*call->qsum[ia]*call->qsum[ib]; sum_lk += lk; gls[idx] = lk; if ( best_lk < lk ) { best_lk = lk; gts[0] = bcf_alleles2gt(call->als_map[ib],call->als_map[ia]); } } } } for (i=0; itrio_Pm_ins<0 && call->trio_Pm_del<0 ) trio_Pm = call->trio_Pm_SNPs; // the same Pm for indels and SNPs requested else { int ret = bcf_get_variant_types(rec); if ( !(ret & VCF_INDEL) ) trio_Pm = call->trio_Pm_SNPs; else { if ( call->trio_Pm_ins<0 ) // dynamic calculation, trio_Pm_del holds the scaling factor { trio_Pm = rec->d.var[1].n<0 ? -21.9313 - 0.2856*rec->d.var[1].n : -22.8689 + 0.2994*rec->d.var[1].n; trio_Pm = 1 - call->trio_Pm_del * exp(trio_Pm); } else // snps and indels set explicitly { trio_Pm = rec->d.var[1].n<0 ? call->trio_Pm_del : call->trio_Pm_ins; } } } // Calculate constrained likelihoods and determine genotypes int ifm; for (ifm=0; ifmnfams; ifm++) { family_t *fam = &call->fams[ifm]; int ntrio = call->ntrio[fam->type][nout_als]; uint16_t *trio = call->trio[fam->type][nout_als]; // Unconstrained likelihood int uc_itr = 0; double uc_lk = 0; for (i=0; i<3; i++) // for father, mother, child { int ismpl = fam->sample[i]; double *gl = call->GLs + ngts*ismpl; if ( gl[0]==1 ) continue; int j, jmax = 0; double max = gl[0]; for (j=1; jsample[i]; double *gl = call->GLs + ngts*ismpl; if ( gl[0]==1 ) continue; int igt = trio[itr]>>((2-i)*4) & 0xf; assert( !call->ploidy || call->ploidy[ismpl]>0 ); if ( igt==GT_SKIP ) continue; lk += gl[igt]; npresent++; // fprintf(stderr," %e", gl[igt]); } // fprintf(stderr,"\t\t"); double Pkij = npresent==3 ? (double)2/(trio[itr]>>12) : 1; // with missing genotypes Pkij's are different lk += log(1 - trio_Pm * (1 - Pkij)); // fprintf(stderr,"%d%d%d\t%e\t%.2f\n", trio[itr]>>8&0xf,trio[itr]>>4&0xf,trio[itr]&0xf, lk, Pkij); if ( c_lk < lk ) { c_lk = lk; c_itr = trio[itr]; } if ( uc_itr==trio[itr] ) uc_is_mendelian = 1; } if ( !uc_is_mendelian ) { uc_lk += log(1 - trio_Pm); //fprintf(stderr,"c_lk=%e uc_lk=%e c_itr=%d%d%d uc_itr=%d%d%d\n", c_lk,uc_lk,c_itr>>8&0xf,c_itr>>4&0xf,c_itr&0xf,uc_itr>>8&0xf,uc_itr>>4&0xf,uc_itr&0xf); if ( c_lk < uc_lk ) { c_lk = uc_lk; c_itr = uc_itr; } } //fprintf(stderr,"best_lk=%e best_itr=%d%d%d uc_itr=%d%d%d\n", c_lk,c_itr>>8&0xf,c_itr>>4&0xf,c_itr&0xf,uc_itr>>8&0xf,uc_itr>>4&0xf,uc_itr&0xf); // Set genotypes for father, mother, child and calculate genotype qualities for (i=0; i<3; i++) { // GT int ismpl = fam->sample[i]; int igt = c_itr>>((2-i)*4) & 0xf; double *gl = call->GLs + ngts*ismpl; int32_t *gts = call->cgts + ismpl; if ( gl[0]==1 || igt==GT_SKIP ) // zero depth, set missing genotypes { gts[0] = bcf_gt_missing; // bcf_float_set_missing(call->GQs[ismpl]); continue; } gts[0] = igt; #if 0 // todo: Genotype Qualities // // GQ: for each family member i sum over all genotypes j,k keeping igt fixed double lk_sum = 0; for (itr=0; itr>((2-i)*4) & 0xf) ) continue; double lk = 0; int j; for (j=0; j<3; j++) { int jsmpl = fam->sample[j]; double *gl = call->GLs + ngts*jsmpl; if ( gl[0]==1 ) continue; int jgt = trio[itr]>>((2-j)*4) & 0xf; if ( jgt==GT_SKIP ) continue; lk += gl[jgt]; } double Pkij = (double)2/(trio[itr]>>12); lk += log(1 - trio_Pm * (1 - Pkij)); lk_sum = logsumexp2(lk_sum, lk); } if ( !uc_is_mendelian && (best_itr>>((2-i)*4)&0xf)==(uc_itr>>((2-i)*4)&0xf) ) lk_sum = logsumexp2(lk_sum,uc_lk); call->GQs[ismpl] = -4.3429*(best_lk - lk_sum); #endif } } for (i=0; i<4; i++) call->ac[i] = 0; call->nhets = 0; call->ndiploid = 0; // Test if CGT,UGT are needed int ucgts_needed = 0; int32_t *cgts = call->cgts - 1; int32_t *ugts = call->ugts - 1; int32_t *gts = call->gts - 2; for (isample = 0; isample < nsmpl; isample++) { int ploidy = call->ploidy ? call->ploidy[isample] : 2; cgts++; ugts++; gts += 2; if ( bcf_gt_is_missing(ugts[0]) ) { gts[0] = bcf_gt_missing; gts[1] = ploidy==2 ? bcf_gt_missing : bcf_int32_vector_end; continue; } int a,b; if ( cgts[0]!=ugts[0] ) { bcf_gt2alleles(cgts[0], &a, &b); gts[0] = bcf_gt_unphased(a); gts[1] = ploidy==1 ? bcf_int32_vector_end : bcf_gt_unphased(b); } else { bcf_gt2alleles(ugts[0], &a, &b); gts[0] = bcf_gt_unphased(a); gts[1] = ploidy==1 ? bcf_int32_vector_end : bcf_gt_unphased(b); } if ( cgts[0]!=ugts[0] ) ucgts_needed = 1; call->ac[a]++; if ( ploidy==2 ) { call->ac[b]++; call->ndiploid++; if ( a!=b ) call->nhets++; } } if ( ucgts_needed ) { // Some GTs are different bcf_update_format_int32(call->hdr,rec,"UGT",call->ugts,nsmpl); bcf_update_format_int32(call->hdr,rec,"CGT",call->cgts,nsmpl); } } static void mcall_trim_PLs(call_t *call, bcf1_t *rec, int nals, int nout_als, int out_als) { int ngts = nals*(nals+1)/2; int npls_src = ngts, npls_dst = nout_als*(nout_als+1)/2; // number of PL values in diploid samples, ori and new if ( call->all_diploid && npls_src == npls_dst ) return; int *pls_src = call->PLs, *pls_dst = call->PLs; int nsmpl = bcf_hdr_nsamples(call->hdr); int isample, ia; for (isample = 0; isample < nsmpl; isample++) { int ploidy = call->ploidy ? call->ploidy[isample] : 2; if ( ploidy==2 ) { for (ia=0; iapl_map[ia] ]; } else if ( ploidy==1 ) { for (ia=0; iapl_map[isrc] ]; } if ( ia1 in mcall() } pls_src += npls_src; pls_dst += npls_dst; } bcf_update_format_int32(call->hdr, rec, "PL", call->PLs, npls_dst*nsmpl); } void mcall_trim_numberR(call_t *call, bcf1_t *rec, int nals, int nout_als, int out_als) { int i, ret; // only DPR so far, we may generalize to arbitrary Number=R if necessary ret = bcf_get_info_int32(call->hdr, rec, "DPR", &call->itmp, &call->n_itmp); if ( ret>0 ) { assert( ret==nals ); if ( out_als==1 ) bcf_update_info_int32(call->hdr, rec, "DPR", call->itmp, 1); else { for (i=0; ials_map[i]==-1 ) continue; // to be dropped call->PLs[ call->als_map[i] ] = call->itmp[i]; // reusing PLs storage which is not used at this point } bcf_update_info_int32(call->hdr, rec, "DPR", call->PLs, nout_als); } } ret = bcf_get_format_int32(call->hdr, rec, "DPR", &call->itmp, &call->n_itmp); if ( ret>0 ) { int nsmpl = bcf_hdr_nsamples(call->hdr); int ndp = ret / nsmpl; assert( ndp==nals ); if ( out_als==1 ) { for (i=0; iPLs[i] = call->itmp[i*ndp]; bcf_update_format_int32(call->hdr, rec, "DPR", call->PLs, nsmpl); } else { int j; for (i=0; iPLs + i*nout_als; int32_t *dp_src = call->itmp + i*ndp; for (j=0; jals_map[j]==-1 ) continue; // to be dropped dp_dst[ call->als_map[j] ] = dp_src[j]; // reusing PLs storage which is not used at this point } } bcf_update_format_int32(call->hdr, rec, "DPR", call->PLs, nsmpl*nout_als); } } } static void mcall_constrain_alleles(call_t *call, bcf1_t *rec, int unseen) { bcf_sr_regions_t *tgt = call->srs->targets; if ( tgt->nals>5 ) error("Maximum accepted number of alleles is 5, got %d\n", tgt->nals); hts_expand(char*,tgt->nals+1,call->nals,call->als); int has_new = 0; int i, j, nals = 1; for (i=1; inals_map; i++) call->als_map[i] = -1; if ( vcmp_set_ref(call->vcmp, rec->d.allele[0], tgt->als[0]) < 0 ) error("The reference alleles are not compatible at %s:%d .. %s vs %s\n", call->hdr->id[BCF_DT_CTG][rec->rid].key,rec->pos+1,tgt->als[0],rec->d.allele[0]); // create mapping from new to old alleles call->als[0] = tgt->als[0]; call->als_map[0] = 0; for (i=1; inals; i++) { call->als[nals] = tgt->als[i]; j = vcmp_find_allele(call->vcmp, rec->d.allele+1, rec->n_allele - 1, tgt->als[i]); if ( j>=0 ) { // existing allele call->als_map[nals] = j+1; } else { // There is a new allele in targets which is not present in VCF. // We use the X allele to estimate PLs. Note that X may not be // present at multiallelic indels sites. In that case we use the // last allele anyway, because the least likely allele comes last // in mpileup's ALT output. call->als_map[nals] = unseen>=0 ? unseen : rec->n_allele - 1; has_new = 1; } nals++; } if ( !has_new && nals==rec->n_allele ) return; bcf_update_alleles(call->hdr, rec, (const char**)call->als, nals); // create mapping from new PL to old PL int k = 0; for (i=0; ials_map[i], b = call->als_map[j]; call->pl_map[k++] = a>b ? a*(a+1)/2 + b : b*(b+1)/2 + a; } } // update PL call->nPLs = bcf_get_format_int32(call->hdr, rec, "PL", &call->PLs, &call->mPLs); int nsmpl = bcf_hdr_nsamples(call->hdr); int npls_ori = call->nPLs / nsmpl; int npls_new = k; hts_expand(int32_t,npls_new*nsmpl,call->n_itmp,call->itmp); int *ori_pl = call->PLs, *new_pl = call->itmp; for (i=0; ipl_map[k]]; if ( new_pl[k]==bcf_int32_missing && unseen>=0 ) { // missing value, and there is an unseen allele: identify the // alleles and use the lk of either AX or XX int k_ori = call->pl_map[k], ia, ib; bcf_gt2alleles(k_ori, &ia, &ib); k_ori = bcf_alleles2gt(ia,unseen); if ( ori_pl[k_ori]==bcf_int32_missing ) k_ori = bcf_alleles2gt(ib,unseen); if ( ori_pl[k_ori]==bcf_int32_missing ) k_ori = bcf_alleles2gt(unseen,unseen); new_pl[k] = ori_pl[k_ori]; } } ori_pl += npls_ori; new_pl += npls_new; } bcf_update_format_int32(call->hdr, rec, "PL", call->itmp, npls_new*nsmpl); // update QS float qsum[5]; int nqs = bcf_get_info_float(call->hdr, rec, "QS", &call->qsum, &call->nqsum); for (i=0; ials_map[i]qsum[call->als_map[i]] : 0; bcf_update_info_float(call->hdr, rec, "QS", qsum, nals); } /** * This function implements the multiallelic calling model. It has two major parts: * 1) determine the most likely set of alleles and calculate the quality of ref/non-ref site * 2) determine and set the genotypes * In various places in between, the BCF record gets updated. */ int mcall(call_t *call, bcf1_t *rec) { int i, unseen = -1; for (i=1; in_allele; i++) { if ( rec->d.allele[i][0]=='X' ) { unseen = i; break; } // old X if ( rec->d.allele[i][0]=='<' && rec->d.allele[i][1]=='X' && rec->d.allele[i][1]=='>' ) { unseen = i; break; } // old if ( rec->d.allele[i][0]=='<' && rec->d.allele[i][1]=='*' && rec->d.allele[i][1]=='>' ) { unseen = i; break; } // new <*> } // Force alleles when calling genotypes given alleles was requested if ( call->flag & CALL_CONSTR_ALLELES ) mcall_constrain_alleles(call, rec, unseen); int nsmpl = bcf_hdr_nsamples(call->hdr); int nals = rec->n_allele; hts_expand(int,nals,call->nals_map,call->als_map); hts_expand(int,nals*(nals+1)/2,call->npl_map,call->pl_map); // Get the genotype likelihoods call->nPLs = bcf_get_format_int32(call->hdr, rec, "PL", &call->PLs, &call->mPLs); if ( call->nPLs!=nsmpl*nals*(nals+1)/2 && call->nPLs!=nsmpl*nals ) // a mixture of diploid and haploid or haploid only error("Wrong number of PL fields? nals=%d npl=%d\n", nals,call->nPLs); // Convert PLs to probabilities int ngts = nals*(nals+1)/2; hts_expand(double, call->nPLs, call->npdg, call->pdg); set_pdg(call->pl2p, call->PLs, call->pdg, nsmpl, ngts, unseen); #if QS_FROM_PDG estimate_qsum(call, rec); #else // Get sum of qualities int nqs = bcf_get_info_float(call->hdr, rec, "QS", &call->qsum, &call->nqsum); if ( nqs<=0 ) error("The QS annotation not present at %s:%d\n", bcf_seqname(call->hdr,rec),rec->pos+1); if ( nqs < nals ) { // Some of the listed alleles do not have the corresponding QS field. This is // typically ref-only site with X in ALT. hts_expand(float,nals,call->nqsum,call->qsum); for (i=nqs; iqsum[i] = 0; } float qsum_tot = 0; for (i=0; iqsum[i]; if ( !call->qsum[0] ) { // As P(RR)!=0 even for QS(ref)=0, we set QS(ref) to a small value, // an equivalent of a single reference read. if ( bcf_get_info_int32(call->hdr, rec, "DP", &call->itmp, &call->n_itmp)!=1 ) error("Could not read DP at %s:%d\n", call->hdr->id[BCF_DT_CTG][rec->rid].key,rec->pos+1); if ( call->itmp[0] ) { call->qsum[0] = 1.0 / call->itmp[0] / nsmpl; qsum_tot += call->qsum[0]; } } if ( qsum_tot ) for (i=0; iqsum[i] /= qsum_tot; #endif // Find the best combination of alleles int out_als, nout = mcall_find_best_alleles(call, nals, &out_als); // Make sure the REF allele is always present if ( !(out_als&1) ) { out_als |= 1; nout++; } int is_variant = out_als==1 ? 0 : 1; if ( call->flag & CALL_VARONLY && !is_variant ) return 0; // With -A, keep all ALTs except X if ( call->flag & CALL_KEEPALT ) { nout = 0; for (i=0; id.allele[i][0]=='X' ) continue; // old version of unseen allele "X" if ( rec->d.allele[i][0]=='<' && rec->d.allele[i][1]=='X' && rec->d.allele[i][2]=='>' ) continue; // old version of unseen allele, "" if ( rec->d.allele[i][0]=='<' && rec->d.allele[i][1]=='*' && rec->d.allele[i][2]=='>' ) continue; // new version of unseen allele, "<*>" out_als |= 1<hdr, rec, "PL", NULL, 0); // remove PL, useless now } else { // The most likely set of alleles includes non-reference allele (or was enforced), call genotypes. // Note that it is a valid outcome if the called genotypes exclude some of the ALTs. init_allele_trimming_maps(call, out_als, nals); if ( !is_variant ) mcall_set_ref_genotypes(call,nals); // running with -A, prevent mcall_call_genotypes from putting some ALT back else if ( call->flag & CALL_CONSTR_TRIO ) mcall_call_trio_genotypes(call, rec, nals,nout,out_als); else mcall_call_genotypes(call,rec,nals,nout,out_als); // Skip the site if all samples are 0/0. This can happen occasionally. nAC = call->ac[1] + call->ac[2] + call->ac[3]; if ( !nAC && call->flag & CALL_VARONLY ) return 0; mcall_trim_PLs(call, rec, nals, nout, out_als); } if ( nals!=nout ) mcall_trim_numberR(call, rec, nals, nout, out_als); // Set QUAL and calculate HWE-related annotations if ( nAC ) { float icb = calc_ICB(call->ac[0],nAC, call->nhets, call->ndiploid); if ( icb != HUGE_VAL ) bcf_update_info_float(call->hdr, rec, "ICB", &icb, 1); float hob = calc_HOB(call->ac[0],nAC, call->nhets, call->ndiploid); if ( hob != HUGE_VAL ) bcf_update_info_float(call->hdr, rec, "HOB", &hob, 1); // Quality of a variant site. fabs() to avoid negative zeros in VCF output when CALL_KEEPALT is set rec->qual = call->lk_sum==-HUGE_VAL ? 0 : fabs(-4.343*(call->ref_lk - call->lk_sum)); } else { // Set the quality of a REF site rec->qual = call->lk_sum==-HUGE_VAL ? 0 : -4.343*log(1 - exp(call->ref_lk - call->lk_sum)); } if ( rec->qual>999 ) rec->qual = 999; if ( rec->qual>50 ) rec->qual = rint(rec->qual); // AC, AN if ( nout>1 ) bcf_update_info_int32(call->hdr, rec, "AC", call->ac+1, nout-1); nAC += call->ac[0]; bcf_update_info_int32(call->hdr, rec, "AN", &nAC, 1); // Remove unused alleles hts_expand(char*,nout,call->nals,call->als); for (i=0; ials_map[i]>=0 ) call->als[call->als_map[i]] = rec->d.allele[i]; bcf_update_alleles(call->hdr, rec, (const char**)call->als, nout); bcf_update_genotypes(call->hdr, rec, call->gts, nsmpl*2); // DP4 tag if ( bcf_get_info_float(call->hdr, rec, "I16", &call->anno16, &call->n16)!=16 ) error("I16 hasn't 16 fields at %s:%d\n", call->hdr->id[BCF_DT_CTG][rec->rid].key,rec->pos+1); int32_t dp[4]; dp[0] = call->anno16[0]; dp[1] = call->anno16[1]; dp[2] = call->anno16[2]; dp[3] = call->anno16[3]; bcf_update_info_int32(call->hdr, rec, "DP4", dp, 4); int32_t mq = (call->anno16[8]+call->anno16[10])/(call->anno16[0]+call->anno16[1]+call->anno16[2]+call->anno16[3]); bcf_update_info_int32(call->hdr, rec, "MQ", &mq, 1); bcf_update_info_int32(call->hdr, rec, "I16", NULL, 0); // remove I16 tag bcf_update_info_int32(call->hdr, rec, "QS", NULL, 0); // remove QS tag return nout; } bcftools-1.2/ploidy.c000066400000000000000000000150111246371514100146350ustar00rootroot00000000000000/* Copyright (C) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include "bcftools.h" #include "ploidy.h" struct _ploidy_t { int nsex, msex; // number of genders, m:number of allocated elements in id2sex array int dflt, min, max; // ploidy: default, min and max (only explicitly listed) regidx_t *idx; void *sex2id; char **id2sex; kstring_t tmp_str; }; regidx_t *ploidy_regions(ploidy_t *ploidy) { return ploidy->idx; } int ploidy_parse(const char *line, char **chr_beg, char **chr_end, reg_t *reg, void *payload, void *usr) { ploidy_t *ploidy = (ploidy_t*) usr; void *sex2id = ploidy->sex2id; // Fill CHR,FROM,TO int i, ret = regidx_parse_tab(line,chr_beg,chr_end,reg,NULL,NULL); if ( ret!=0 ) return ret; // Skip the fields already parsed by regidx_parse_tab char *ss = (char*) line; while ( *ss && isspace(*ss) ) ss++; for (i=0; i<3; i++) { while ( *ss && !isspace(*ss) ) ss++; if ( !*ss ) return -2; // wrong number of fields while ( *ss && isspace(*ss) ) ss++; } if ( !*ss ) return -2; // Parse the payload char *se = ss; while ( *se && !isspace(*se) ) se++; if ( !*se || se==ss ) error("Could not parse: %s\n", line); ploidy->tmp_str.l = 0; kputsn(ss,se-ss,&ploidy->tmp_str); sex_ploidy_t *sp = (sex_ploidy_t*) payload; if ( khash_str2int_get(sex2id, ploidy->tmp_str.s, &sp->sex) != 0 ) { ploidy->nsex++; hts_expand0(char*,ploidy->nsex,ploidy->msex,ploidy->id2sex); ploidy->id2sex[ploidy->nsex-1] = strdup(ploidy->tmp_str.s); sp->sex = khash_str2int_inc(ploidy->sex2id, ploidy->id2sex[ploidy->nsex-1]); } ss = se; while ( *se && isspace(*se) ) se++; if ( !*se ) error("Could not parse: %s\n", line); sp->ploidy = strtol(ss,&se,10); if ( ss==se ) error("Could not parse: %s\n", line); if ( sp->ploidy < ploidy->min ) ploidy->min = sp->ploidy; if ( sp->ploidy > ploidy->max ) ploidy->max = sp->ploidy; return 0; } ploidy_t *ploidy_init(const char *fname, int dflt) { ploidy_t *pld = (ploidy_t*) calloc(1,sizeof(ploidy_t)); if ( !pld ) return NULL; pld->dflt = pld->min = pld->max = dflt; pld->sex2id = khash_str2int_init(); pld->idx = regidx_init(fname,ploidy_parse,NULL,sizeof(sex_ploidy_t),pld); if ( !pld->idx ) { ploidy_destroy(pld); pld = NULL; } return pld; } ploidy_t *ploidy_init_string(const char *str, int dflt) { ploidy_t *pld = (ploidy_t*) calloc(1,sizeof(ploidy_t)); if ( !pld ) return NULL; pld->dflt = pld->min = pld->max = dflt; pld->sex2id = khash_str2int_init(); pld->idx = regidx_init(NULL,ploidy_parse,NULL,sizeof(sex_ploidy_t),pld); kstring_t tmp = {0,0,0}; const char *ss = str; while ( *ss ) { while ( *ss && isspace(*ss) ) ss++; const char *se = ss; while ( *se && *se!='\r' && *se!='\n' ) se++; tmp.l = 0; kputsn(ss, se-ss, &tmp); regidx_insert(pld->idx,tmp.s); while ( *se && isspace(*se) ) se++; ss = se; } regidx_insert(pld->idx,NULL); free(tmp.s); return pld; } void ploidy_destroy(ploidy_t *ploidy) { if ( ploidy->sex2id ) khash_str2int_destroy_free(ploidy->sex2id); if ( ploidy->idx ) regidx_destroy(ploidy->idx); free(ploidy->id2sex); free(ploidy->tmp_str.s); free(ploidy); } int ploidy_query(ploidy_t *ploidy, char *seq, int pos, int *sex2ploidy, int *min, int *max) { regitr_t itr; int i, ret = regidx_overlap(ploidy->idx, seq,pos,pos, &itr); if ( !sex2ploidy && !min && !max ) return ret; if ( !ret ) { // no overlap if ( min ) *min = ploidy->dflt; if ( max ) *max = ploidy->dflt; if ( sex2ploidy ) for (i=0; insex; i++) sex2ploidy[i] = ploidy->dflt; return 0; } int _min = INT_MAX, _max = -1; if ( sex2ploidy ) for (i=0; insex; i++) sex2ploidy[i] = ploidy->dflt; while ( REGITR_OVERLAP(itr,pos,pos) ) { int sex = REGITR_PAYLOAD(itr,sex_ploidy_t).sex; int pld = REGITR_PAYLOAD(itr,sex_ploidy_t).ploidy; if ( pld!=ploidy->dflt ) { if ( sex2ploidy ) sex2ploidy[ sex ] = pld; if ( _min > pld ) _min = pld; if ( _max < pld ) _max = pld; } itr.i++; } if ( _max==-1 ) _max = _min = ploidy->dflt; if ( max ) *max = _max; if ( min ) *min = _min; return 1; } int ploidy_nsex(ploidy_t *ploidy) { return ploidy->nsex; } char *ploidy_id2sex(ploidy_t *ploidy, int id) { if ( id<0 || id>=ploidy->nsex ) return NULL; return ploidy->id2sex[id]; } int ploidy_sex2id(ploidy_t *ploidy, char *sex) { int id; if ( khash_str2int_get(ploidy->sex2id,sex,&id)!=0 ) return -1; return id; } int ploidy_add_sex(ploidy_t *ploidy, const char *sex) { int id; if ( khash_str2int_get(ploidy->sex2id, sex, &id)==0 ) return id; ploidy->nsex++; hts_expand0(char*,ploidy->nsex,ploidy->msex,ploidy->id2sex); ploidy->id2sex[ploidy->nsex-1] = strdup(sex); return khash_str2int_inc(ploidy->sex2id, ploidy->id2sex[ploidy->nsex-1]); } int ploidy_max(ploidy_t *ploidy) { return ploidy->dflt > ploidy->max ? ploidy->dflt : ploidy->max; } int ploidy_min(ploidy_t *ploidy) { return ploidy->dflt < ploidy->min ? ploidy->dflt : ploidy->min; } bcftools-1.2/ploidy.h000066400000000000000000000102351246371514100146450ustar00rootroot00000000000000/* Copyright (C) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ /* Lookup from region and sex to ploidy. Example of usage: int default_ploidy = 2; ploidy_t *pld = ploidy_init(fname, default_ploidy); int nsex = ploidy_nsex(pld); int *sex2ploidy = malloc(sizeof(int)*nsex); ploidy_query(pld, "X",60000, sex2ploidy, NULL, NULL); for (i=0; i typedef struct _ploidy_t ploidy_t; typedef struct { int sex, ploidy; } sex_ploidy_t; /* * ploidy_init() * @param fname: input file name or NULL if default ploidy from example above should be used * @param dflt: default ploidy to use for unlisted regions * * Returns new structure on success or NULL on error. */ ploidy_t *ploidy_init(const char *fname, int dflt); /* Same as ploidy_init() but the whole file is passed as a single string */ ploidy_t *ploidy_init_string(const char *str, int dflt); /* * ploidy_destroy() - free memory allocated by ploidy_init */ void ploidy_destroy(ploidy_t *ploidy); /* * ploidy_query() - query ploidy at a position for all genders at once * @param seq: chromosome name * @param pos: 0-based position * @param sex2ploidy: if not NULL, array will be filled with mapping from sex id to ploidy * @param min: if not NULL, minimum encountered encountered will be set * @param max: if not NULL, maximum encountered encountered will be set * * Returns 1 if the position is listed in the regions or 0 otherwise. */ int ploidy_query(ploidy_t *ploidy, char *seq, int pos, int *sex2ploidy, int *min, int *max); /* * ploidy_nsex() - return number of recognised genders */ int ploidy_nsex(ploidy_t *ploidy); /* * ploidy_id2sex() - mapping between numeric gender id and name * * Returns gender name (e.g. "M" or "F" in the example above) * or NULL if there is no such id. */ char *ploidy_id2sex(ploidy_t *ploidy, int id); /* * ploidy_sex2id() - mapping between gender name and its numeric id * * Returns numeric id or -1 if not present. */ int ploidy_sex2id(ploidy_t *ploidy, char *sex); /* * ploidy_add_sex() - register new gender name. This function is * useful when gender has the default ploidy for all regions * and is not listed in the file passed to ploidy_init() * * Returns numeric id of the added sex, regardless of whether the string was * newly added or was already present in the dictionary. */ int ploidy_add_sex(ploidy_t *ploidy, const char *sex); /** Returns region index for raw access */ regidx_t *ploidy_regions(ploidy_t *ploidy); /** Return the minimum / maximum recognised ploidy */ int ploidy_max(ploidy_t *ploidy); int ploidy_min(ploidy_t *ploidy); #endif bcftools-1.2/plot-vcfstats000077500000000000000000002351741246371514100157460ustar00rootroot00000000000000#!/usr/bin/env perl # # Copyright (C) 2012-2014 Genome Research Ltd. # # Author: Petr Danecek # # Permission is hereby granted, free of charge, to any person obtaining a copy # of this software and associated documentation files (the "Software"), to deal # in the Software without restriction, including without limitation the rights # to use, copy, modify, merge, publish, distribute, sublicense, and/or sell # copies of the Software, and to permit persons to whom the Software is # furnished to do so, subject to the following conditions: # # The above copyright notice and this permission notice shall be included in # all copies or substantial portions of the Software. # # THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR # IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, # FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL # THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER # LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING # FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER # DEALINGS IN THE SOFTWARE. # Dependencies: # - matplotlib # http://matplotlib.sourceforge.net # - LaTex/xcolor.sty # Download .sty.gz LaTeX class style from http://www.ukern.de/tex/xcolor.html, # unpack and install system-wide or place elsewhere and make available by # setting the TEXINPUTS environment variable (note the colon) # export TEXINPUTS=some/dir: # The list of the recognised path can be obtained from `kpsepath tex` # # use strict; use warnings; use Carp; use Storable qw(dclone); my $opts = parse_params(); parse_vcfstats($opts); merge_vcfstats($opts) if @{$$opts{vcfstats}} > 1; if ( $$opts{make_plots} ) { init_plots($opts); plot_venn_bars($opts); plot_counts_by_AF($opts); plot_overlap_by_AF($opts); plot_concordance_by_AF($opts); plot_concordance_by_sample($opts); for my $id (file_ids($opts)) { # irc plots will be deprecated # plot_irc_by_AF($opts,$id); # plot_irc_by_rlen($opts,$id); plot_tstv_by_AF($opts,$id); plot_tstv_by_QUAL($opts,$id); plot_indel_distribution($opts,$id); plot_substitutions($opts,$id); plot_per_sample_stats($opts,$id); plot_DP($opts,$id); plot_hwe($opts,$id); } plot($opts); } create_pdf($opts) unless !$$opts{make_pdf}; exit; #-------------------------------- sub error { my (@msg) = @_; if ( scalar @msg ) { confess @msg; } die "Usage: plot-vcfstats [OPTIONS] file.chk ...\n", " plot-vcfstats -p outdir/ file.chk ...\n", "Options:\n", " -m, --merge Merge vcfstats files to STDOUT, skip plotting.\n", " -p, --prefix The output files prefix, add a slash to create new directory.\n", " -P, --no-PDF Skip the PDF creation step.\n", " -r, --rasterize Rasterize PDF images for fast rendering.\n", " -s, --sample-names Use sample names for xticks rather than numeric IDs.\n", " -t, --title Identify files by these titles in plots. Can be given multiple times.\n", " -T, --main-title Main title for the PDF.\n", " -h, -?, --help This help message.\n", "\n"; } sub parse_params { $0 =~ s{^.+/}{}; my $opts = { pdf_plots => 1, use_sample_names => 0, verbose => 1, make_pdf => 1, make_plots => 1, merge => 0, args => join(' ',$0,@ARGV), img_width => 11/2.54, img_height => 10/2.54, id2col => [ 'orange', 'red', 'darkgreen' ], tex => { slide3v => { height1 => '7cm', height2 => '7cm', height3 => '4.5cm' }, slide3h => { width1 => '15cm', width2 => '10cm', width3 => '8cm' }, }, # for file version sanity check sections => [ { id=>'ID', header=>'Definition of sets', exp=>"# ID\t[2]id\t[3]tab-separated file names" }, { id=>'SN', header=>'SN, Summary numbers', exp=>"# SN [2]id [3]key [4]value" }, { id=>'TSTV', header=>'# TSTV, transition/transversions:', exp=>"# TSTV\t[2]id\t[3]ts\t[4]tv\t[5]ts/tv\t[6]ts (1st ALT)\t[7]tv (1st ALT)\t[8]ts/tv (1st ALT)" }, { id=>'SiS', header=>'Sis, Singleton stats', exp=>"# SiS\t[2]id\t[3]allele count\t[4]number of SNPs\t[5]number of transitions\t[6]number of transversions\t[7]number of indels\t[8]repeat-consistent\t[9]repeat-inconsistent\t[10]not applicable" }, { id=>'AF', header=>'AF, Stats by non-reference allele frequency', exp=>"# AF\t[2]id\t[3]allele frequency\t[4]number of SNPs\t[5]number of transitions\t[6]number of transversions\t[7]number of indels\t[8]repeat-consistent\t[9]repeat-inconsistent\t[10]not applicable" }, { id=>'IDD', header=>'IDD, InDel distribution', exp=>"# IDD\t[2]id\t[3]length (deletions negative)\t[4]count" }, { id=>'ST', header=>'ST, Substitution types', exp=>"# ST\t[2]id\t[3]type\t[4]count" }, { id=>'GCsAF', header=>'GCsAF, Genotype concordance by non-reference allele frequency (SNPs)', exp=>"# GCsAF\t[2]id\t[3]allele frequency\t[4]RR Hom matches\t[5]RA Het matches\t[6]AA Hom matches\t[7]RR Hom mismatches\t[8]RA Het mismatches\t[9]AA Hom mismatches\t[10]dosage r-squared\t[11]number of sites" }, { id=>'GCiAF', header=>'GCiAF, Genotype concordance by non-reference allele frequency (indels)', exp=>"# GCiAF\t[2]id\t[3]allele frequency\t[4]RR Hom matches\t[5]RA Het matches\t[6]AA Hom matches\t[7]RR Hom mismatches\t[8]RA Het mismatches\t[9]AA Hom mismatches\t[10]dosage r-squared\t[11]number of sites" }, { id=>'NRDs', header=>'Non-Reference Discordance (NRD), SNPs', exp=>"# NRDs\t[2]id\t[3]NRD\t[4]Ref/Ref discordance\t[5]Ref/Alt discordance\t[6]Alt/Alt discordance" }, { id=>'NRDi', header=>'Non-Reference Discordance (NRD), indels', exp=>"# NRDi\t[2]id\t[3]NRD\t[4]Ref/Ref discordance\t[5]Ref/Alt discordance\t[6]Alt/Alt discordance" }, { id=>'GCsS', header=>'GCsS, Genotype concordance by sample (SNPs)', exp=>"# GCsS\t[2]id\t[3]sample\t[4]non-reference discordance rate\t[5]RR Hom matches\t[6]RA Het matches\t[7]AA Hom matches\t[8]RR Hom mismatches\t[9]RA Het mismatches\t[10]AA Hom mismatches" }, { id=>'GCiS', header=>'GCiS, Genotype concordance by sample (indels)', exp=>"# GCiS\t[2]id\t[3]sample\t[4]non-reference discordance rate\t[5]RR Hom matches\t[6]RA Het matches\t[7]AA Hom matches\t[8]RR Hom mismatches\t[9]RA Het mismatches\t[10]AA Hom mismatches" }, { id=>'PSC', header=>'PSC, Per-sample counts', exp=>"# PSC\t[2]id\t[3]sample\t[4]nRefHom\t[5]nNonRefHom\t[6]nHets\t[7]nTransitions\t[8]nTransversions\t[9]nIndels\t[10]average depth\t[11]nSingletons" }, { id=>'PSI', header=>'PSI, Per-sample Indels', exp=>"# PSI\t[2]id\t[3]sample\t[4]in-frame\t[5]out-frame\t[6]not applicable\t[7]out/(in+out) ratio" }, { id=>'DP', header=>'DP, Depth distribution', exp=>"# DP\t[2]id\t[3]bin\t[4]number of genotypes\t[5]fraction of genotypes (%)\t[6]number of sites\t[7]fraction of sites (%)" }, { id=>'FS', header=>'FS, Indel frameshifts', exp=>"# FS\t[2]id\t[3]in-frame\t[4]out-frame\t[5]not applicable\t[6]out/(in+out) ratio\t[7]in-frame (1st ALT)\t[8]out-frame (1st ALT)\t[9]not applicable (1st ALT)\t[10]out/(in+out) ratio (1st ALT)" }, { id=>'ICS', header=>'ICS, Indel context summary', exp=>"# ICS\t[2]id\t[3]repeat-consistent\t[4]repeat-inconsistent\t[5]not applicable\t[6]c/(c+i) ratio" }, { id=>'ICL', header=>'ICL, Indel context by length', exp=>"# ICL\t[2]id\t[3]length of repeat element\t[4]repeat-consistent deletions)\t[5]repeat-inconsistent deletions\t[6]consistent insertions\t[7]inconsistent insertions\t[8]c/(c+i) ratio" }, { id=>'QUAL', header=>'QUAL, Stats by quality', exp=>"# QUAL\t[2]id\t[3]Quality\t[4]number of SNPs\t[5]number of transitions (1st ALT)\t[6]number of transversions (1st ALT)\t[7]number of indels" }, { id=>'HWE', header=>'HWE', exp=>"# HWE\t[2]id\t[3]1st ALT allele frequency\t[4]Number of observations\t[5]25th percentile\t[6]median\t[7]75th percentile", }, ], }; for my $sec (@{$$opts{sections}}) { $$opts{exp}{$$sec{id}} = $$sec{exp}; $$opts{id2sec}{$$sec{id}} = $sec; } while (defined(my $arg=shift(@ARGV))) { if ( $arg eq '--no-plots' ) { $$opts{make_plots}=0; next; } if ( $arg eq '-P' || $arg eq '--no-PDF' ) { $$opts{make_pdf}=0; next; } if ( $arg eq '-r' || $arg eq '--rasterize' ) { $$opts{rasterize}=1; $$opts{pdf_plots} = 0; next; } if ( $arg eq '-m' || $arg eq '--merge' ) { $$opts{make_plots}=0; $$opts{make_pdf}=0; $$opts{merge}=1; next; } if ( $arg eq '-s' || $arg eq '--sample-names' ) { $$opts{use_sample_names}=1; next; } if ( $arg eq '-t' || $arg eq '--title' ) { push @{$$opts{titles}},shift(@ARGV); next; } if ( $arg eq '-T' || $arg eq '--main-title' ) { $$opts{main_title} = shift(@ARGV); next; } if ( $arg eq '-p' || $arg eq '--prefix' ) { $$opts{prefix}=shift(@ARGV); next; } if ( $arg eq '-?' || $arg eq '-h' || $arg eq '--help' ) { error(); } if ( -e $arg ) { push @{$$opts{vcfstats}},$arg; next; } error("Unknown parameter or non-existent file \"$arg\". Run -h for help.\n"); } if ( !exists($$opts{vcfstats}) ) { error(); } if ( !exists($$opts{prefix}) ) { if ( !$$opts{merge} ) { error("Expected -p parameter.\n") } $$opts{prefix} = './'; } elsif ( $$opts{merge} ) { error("Only one of -p or -m should be given.\n"); } if ( $$opts{merge} && @{$$opts{vcfstats}} < 2 ) { error("Nothing to merge\n") } $$opts{dir} = $$opts{prefix}; $$opts{dir} =~ s{/[^/]+$}{/}; if ( !($$opts{dir}=~m{/$}) ) { $$opts{dir} = './'; } if ( !($$opts{prefix}=~m{/$}) && !($$opts{prefix}=~/-$/) ) { $$opts{prefix} .= '-'; } $$opts{lprefix} = $$opts{prefix}; $$opts{lprefix} =~ s{^.+/}{}; $$opts{logfile} = "$$opts{lprefix}plot-vcfstats.log"; if ( !-d $$opts{dir} ) { `mkdir -p $$opts{dir}`; } `> $$opts{dir}$$opts{logfile}` unless $$opts{merge}; return $opts; } sub plot { my ($opts) = @_; if ( !exists($$opts{plt_fh}) ) { return; } close($$opts{plt_fh}) or error("close $$opts{plt_fh}"); my $cmd = "python $$opts{plt_file}"; print STDERR "Plotting graphs: $cmd\n" unless !$$opts{verbose}; system($cmd); if ( $? ) { error("The command exited with non-zero status $?:\n\t$cmd\n\n"); } } sub parse_vcfstats { my ($opts) = @_; for (my $i=0; $i<@{$$opts{vcfstats}}; $i++) { parse_vcfstats1($opts,$i); } # Check sanity if ( !exists($$opts{dat}{ID}{0}) ) { error("Sanity check failed: no stats found by vcfstats??\n"); } # Set titles my %file2title; my %title2file; if ( exists($$opts{titles}) ) { for (my $i=0; $i<@{$$opts{titles}}; $i++) { if ( !exists($$opts{dat}{ID}{$i}) ) { next; } $file2title{$$opts{dat}{ID}{$i}[0][0]} = $$opts{titles}[$i]; $title2file{$$opts{titles}[$i]} = $$opts{dat}{ID}{$i}[0][0]; } } for my $id (file_ids($opts)) { if ( @{$$opts{dat}{ID}{$id}[0]}>1 ) { next; } # shared stats of two files my $file = $$opts{dat}{ID}{$id}[0][0]; if ( !exists($file2title{$file}) ) # create short title { my $bname = $file; $bname =~ s{^.*/}{}; $bname =~ s{\.vcf\.gz$}{}i; if ( length($bname) > 5 ) { $bname = substr($bname,0,5); } my $i = 0; my $title = $bname; while ( exists($title2file{$title}) ) { $title = $bname.chr(66+$i); $i++; } $file2title{$file} = $title; $title2file{$title} = $file; } } for my $id (file_ids($opts)) { my @titles; for my $file (@{$$opts{dat}{ID}{$id}[0]}) { push @titles, $file2title{$file} if $file2title{$file}; } $$opts{title}{$id} = join(' + ',@titles); } # mapping from file names to list of IDs for my $id (file_ids($opts)) { my @files; for my $file (@{$$opts{dat}{ID}{$id}[0]}) { push @{$$opts{file2ids}{$file}}, $id; } } # check sanity of the file merge: were the correct files merged? if ( exists($$opts{coalesced_files}) && $$opts{verbose} ) { print STDERR "The vcfstats outputs have been merged as follows:\n"; my %printed; for my $id (keys %{$$opts{coalesced_files}}) { for (my $i=0; $i<@{$$opts{coalesced_files}{$id}}; $i++) { for (my $j=0; $j<@{$$opts{coalesced_files}{$id}[$i]}; $j++) { if ( exists($printed{$$opts{dat}{ID}{$id}[$i][$j]}) ) { next; } print STDERR "\t$$opts{dat}{ID}{$id}[$i][$j]\n"; for my $file (keys %{$$opts{coalesced_files}{$id}[$i][$j]}) { my $n = $$opts{coalesced_files}{$id}[$i][$j]{$file}; print STDERR "\t\t$file", ($n>1 ? "\t..\t${n}x" : ''),"\n"; } $printed{$$opts{dat}{ID}{$id}[$i][$j]} = 1; } } } } } sub add_to_values { my ($dst,$src,$cmp) = @_; my $id = 0; my $is = 0; while ($is<@$src) { while ( $id<@$dst && &$cmp($$src[$is][0],$$dst[$id][0])>0 ) { $id++; } if ( $id<@$dst && !&$cmp($$src[$is][0],$$dst[$id][0]) ) { for (my $j=1; $j<@{$$src[$is]}; $j++) { $$dst[$id][$j] += $$src[$is][$j]; } } else { splice(@$dst,$id,0,$$src[$is]); } $is++; } } sub add_to_sample_values { my ($dst,$src) = @_; my %id2i; for (my $i=0; $i<@$dst; $i++) { $id2i{$$dst[$i][0]} = $i; } for (my $i=0; $i<@$src; $i++) { if ( !exists($id2i{$$src[$i][0]}) ) { error("Whoops, no such dst sample: $$src[$i][0]\n"); } my $di = $id2i{$$src[$i][0]}; for (my $j=1; $j<@{$$src[$i]}; $j++) { $$dst[$di][$j] += $$src[$i][$j]; } } } sub merge_PSC { my ($a,$b,$n) = @_; for (my $i=0; $i<@$a; $i++) { $$a[$i][7] *= $n; } # average DP add_to_sample_values($a,$b); for (my $i=0; $i<@$a; $i++) { $$a[$i][7] /= $n+1; } } sub merge_PSI { my ($a,$b,$n) = @_; add_to_sample_values($a,$b); for (my $i=0; $i<@$b; $i++) { $$a[$i][4] = sprintf "%.2f", ($$a[$i][1]+$$a[$i][2] ? $$a[$i][2]/($$a[$i][1]+$$a[$i][2]) : 0); } } sub rglob { my ($a,$b) = @_; if ( $a eq $b ) { return $a; } $a =~ s/\*//; my $la = length($a); my $lb = length($b); my $i = 0; while ( $i<$la && $i<$lb && substr($a,$i,1) eq substr($b,$i,1) ) { $i++; } $la--; $lb--; while ( $la>$i && $lb>$i && substr($a,$la,1) eq substr($b,$lb,1) ) { $la--; $lb--; } $la = $la==$i && $lb==$i ? 1 : $la-$i; substr($a,$i,$la,'*'); return $a; } sub merge_id { # merge id filenames my ($opts,$dst,$src,$id) = @_; for (my $i=0; $i<@{$$src{$id}}; $i++) { for (my $j=0; $j<@{$$src{$id}[$i]}; $j++) { my $gname = rglob($$dst{$id}[$i][$j], $$src{$id}[$i][$j]); $$dst{$id}[$i][$j] = $gname; $$opts{coalesced_files}{$id}[$i][$j]{$$src{$id}[$i][$j]}++; } } } sub add_to_avg { my ($dst,$src,$n) = @_; for (my $i=0; $i<@$src; $i++) { if ( ref($$dst[$i]) eq 'ARRAY' ) { for (my $j=0; $j<@{$$dst[$i]}; $j++) { $$dst[$i][$j] = ($n*$$dst[$i][$j]+$$src[$i][$j])/($n+1); } } else { $$dst[$i] = ($n*$$dst[$i]+$$src[$i])/($n+1); } } } sub cmp_str($$) { my ($a,$b) = @_; return $a cmp $b; } sub cmp_num($$) { my ($a,$b) = @_; return $a <=> $b; } sub cmp_num_op($$) { # numeric compare with operators # Cases like <3, >500 make it complicated my ($a,$b) = @_; my $xa = '='; my $xb = '='; if ( $a=~/^(\D+)/ ) { $xa = $1; $a = $'; } if ( $b=~/^(\D+)/ ) { $xb = $1; $b = $'; } if ( $a==$b ) { return $xa cmp $xb; } $a <=> $b; } sub merge_dp { my ($a,$b) = @_; add_to_values($a,$b,\&cmp_num_op); # recalculate fraction of GTs, cannot be simply summed my $sum = 0; for (my $i=0; $i<@$a; $i++) { $sum += $$a[$i][1]; } for (my $i=0; $i<@$a; $i++) { $$a[$i][2] = $$a[$i][1]*100./$sum; } } sub merge_GCsS { my ($a,$b,$n) = @_; # average the non-ref discordance rate for (my $i=0; $i<@$a; $i++) { $$a[$i][1] *= $n; } add_to_sample_values($a,$b); for (my $i=0; $i<@$a; $i++) { $$a[$i][1] /= $n+1; } } sub merge_FS { my ($a,$b) = @_; for (my $i=0; $i<@$a; $i++) { for (my $j=0; $j<3; $j++) { $$a[$i][$j] += $$b[$i][$j]; } $$a[$i][3] = sprintf "%.2f", ($$a[$i][0] + $$a[$i][1]) ? $$a[$i][1]/($$a[$i][0] + $$a[$i][1]) : 0; for (my $j=4; $j<7; $j++) { $$a[$i][$j] += $$b[$i][$j]; } $$a[$i][7] = sprintf "%.2f", ($$a[$i][4] + $$a[$i][5]) ? $$a[$i][5]/($$a[$i][4] + $$a[$i][5]) : 0; } } sub merge_ICS { my ($a,$b) = @_; for (my $i=0; $i<@$a; $i++) { for (my $j=0; $j<3; $j++) { $$a[$i][$j] += $$b[$i][$j]; } $$a[$i][3] = sprintf "%.4f", ($$a[$i][0] + $$a[$i][1]) ? $$a[$i][0]/($$a[$i][0] + $$a[$i][1]) : 0; } } sub merge_ICL { my ($a,$b) = @_; for (my $i=0; $i<@$a; $i++) { for (my $j=1; $j<5; $j++) { $$a[$i][$j] += $$b[$i][$j]; } $$a[$i][5] = sprintf "%.4f", ($$a[$i][2] + $$a[$i][4]) ? ($$a[$i][1] + $$a[$i][3])/($$a[$i][1] + $$a[$i][2] + $$a[$i][3] + $$a[$i][4]) : 0; } } sub merge_TSTV { my ($a,$b) = @_; for (my $i=0; $i<@$a; $i++) { for (my $j=0; $j<2; $j++) { $$a[$i][$j] += $$b[$i][$j]; } $$a[$i][2] = sprintf "%.2f", $$a[$i][1] ? $$a[$i][0]/$$a[$i][1] : 0; for (my $j=3; $j<5; $j++) { $$a[$i][$j] += $$b[$i][$j]; } $$a[$i][5] = sprintf "%.2f", $$a[$i][4] ? $$a[$i][3]/$$a[$i][4] : 0; } } sub merge_GCsAF { my ($a,$b) = @_; # recalculate r2 for (my $i=0; $i<@$a; $i++) { $$a[$i][7] *= $$a[$i][8]; } for (my $i=0; $i<@$b; $i++) { $$b[$i][7] *= $$b[$i][8]; } add_to_values($a,$b,\&cmp_num_op); for (my $i=0; $i<@$a; $i++) { $$a[$i][7] /= $$a[$i][8]; } } sub parse_vcfstats1 { my ($opts,$i) = @_; my $file = $$opts{vcfstats}[$i]; print STDERR "Parsing bcftools stats output: $file\n" unless !$$opts{verbose}; open(my $fh,'<',$file) or error("$file: $!"); my $line = <$fh>; if ( !$line or !($line=~/^# This file was produced by \S*/) ) { error("Sanity check failed: was this file generated by bcftools stats?"); } my %dat; while ($line=<$fh>) { $line =~ s/\s*$//; if ( $line=~/^#\s+(\S+)\t/ ) { $$opts{def_line}{$1} = $line; next; } if ( $line=~/^#/ ) { next; } my @items = split(/\t/,$line); if ( $items[0] eq 'SN' ) { $dat{$items[1]}{$items[2]} = splice(@items,3); next; } push @{$dat{$items[0]}{$items[1]}}, [splice(@items,2)]; } close($fh); for my $a (keys %dat) { if ( !exists($$opts{dat}{$a}) ) { $$opts{dat}{$a} = $dat{$a}; next; } # first vcfstats file for my $b (keys %{$dat{$a}}) { # Merging multiple vcfstats files. Honestly, this is quite hacky. if ( !exists($$opts{dat}{$a}{$b}) ) { $$opts{dat}{$a}{$b} = $dat{$a}{$b}; next; } # copy all, first occurance if ( $a eq 'ID' ) { merge_id($opts,$$opts{dat}{$a},$dat{$a},$b); } elsif ( ref($dat{$a}{$b}) ne 'ARRAY' ) { $$opts{dat}{$a}{$b} += $dat{$a}{$b} unless $b eq 'number of samples:'; } # SN, Summary numbers, do not sum sample counts elsif ( $a eq 'NRDs' ) { add_to_avg($$opts{dat}{$a}{$b},$dat{$a}{$b},$i); } elsif ( $a eq 'NRDi' ) { add_to_avg($$opts{dat}{$a}{$b},$dat{$a}{$b},$i); } elsif ( $a eq 'DP' ) { merge_dp($$opts{dat}{$a}{$b},$dat{$a}{$b}); } elsif ( $a eq 'GCsS' ) { merge_GCsS($$opts{dat}{$a}{$b},$dat{$a}{$b},$i); } elsif ( $a eq 'GCiS' ) { merge_GCsS($$opts{dat}{$a}{$b},$dat{$a}{$b},$i); } elsif ( $a eq 'GCsAF' ) { merge_GCsAF($$opts{dat}{$a}{$b},$dat{$a}{$b},$i); } elsif ( $a eq 'GCiAF' ) { merge_GCsAF($$opts{dat}{$a}{$b},$dat{$a}{$b},$i); } elsif ( $a eq 'ST' ) { add_to_values($$opts{dat}{$a}{$b},$dat{$a}{$b},\&cmp_str); } elsif ( $a eq 'PSC') { merge_PSC($$opts{dat}{$a}{$b},$dat{$a}{$b},$i); } elsif ( $a eq 'PSI') { merge_PSI($$opts{dat}{$a}{$b},$dat{$a}{$b},$i); } elsif ( $a eq 'IDD') { add_to_values($$opts{dat}{$a}{$b},$dat{$a}{$b},\&cmp_num); } elsif ( $a eq 'FS') { merge_FS($$opts{dat}{$a}{$b},$dat{$a}{$b}); } elsif ( $a eq 'ICS') { merge_ICS($$opts{dat}{$a}{$b},$dat{$a}{$b}); } elsif ( $a eq 'ICL') { merge_ICL($$opts{dat}{$a}{$b},$dat{$a}{$b}); } elsif ( $a eq 'TSTV') { merge_TSTV($$opts{dat}{$a}{$b},$dat{$a}{$b},$i); } elsif ( $a eq 'DBG' ) { next; } else { add_to_values($$opts{dat}{$a}{$b},$dat{$a}{$b},\&cmp_num_op); } # SiS AF IDD } } } sub file_ids { my ($opts) = @_; my $id = 0; my @out; while ( exists($$opts{dat}{ID}) && exists($$opts{dat}{ID}{$id}) ) { push @out, $id++; } return @out; } sub tprint { my ($fh,@txt) = @_; for my $txt (@txt) { $txt =~ s/\n[ \t]+/\n/g; # eat leading tabs while ( ($txt =~ /\n\t*\\t/) ) { $txt =~ s/(\n\t*)\\t/$1\t/g; # replace escaped tabs (\\t) with tabs } print $fh $txt; } } sub init_plots { my ($opts) = @_; $$opts{plt_file} = "$$opts{prefix}plot.py"; my $titles = "# Title abbreviations:\n"; for my $id (file_ids($opts)) { $titles .= "# \t $id .. $$opts{title}{$id} .. $$opts{dat}{ID}{$id}[0][0]\n"; } $titles .= "#"; open(my $fh,'>',$$opts{plt_file}) or error("$$opts{plt_file}: $!"); tprint $fh, qq[ # This file was produced by plot-vcfstats, the command line was: # $$opts{args} # # Edit as necessary and recreate the plots by running # python $$opts{plt_file} # $titles # Set to 1 to plot in PDF instead of PNG pdf_plots = $$opts{pdf_plots} # Plots to generate, set to 0 to disable plot_venn_snps = 1 plot_venn_indels = 1 plot_tstv_by_sample = 1 plot_hethom_by_sample = 1 plot_snps_by_sample = 1 plot_indels_by_sample = 1 plot_singletons_by_sample = 1 plot_depth_by_sample = 1 plot_SNP_count_by_af = 1 plot_Indel_count_by_af = 1 plot_SNP_overlap_by_af = 1 plot_Indel_overlap_by_af = 1 plot_dp_dist = 1 plot_hwe = 1 plot_concordance_by_af = 1 plot_r2_by_af = 1 plot_discordance_by_sample = 1 plot_tstv_by_af = 1 plot_indel_dist = 1 plot_tstv_by_qual = 1 plot_substitutions = 1 # Set to 1 to use sample names for xticks instead of numeric sequential IDs # and adjust margins and font properties if necessary sample_names = $$opts{use_sample_names} sample_margins = {'right':0.98, 'left':0.07, 'bottom':0.2} sample_font = {'rotation':45, 'ha':'right', 'fontsize':8} if sample_names==0: sample_margins=(); sample_font=(); #------------------------------------------------- import matplotlib as mpl mpl.use('Agg') import matplotlib.pyplot as plt import csv csv.register_dialect('tab', delimiter='\\t', quoting=csv.QUOTE_NONE) import numpy def smooth(x,window_len=11,window='hanning'): \\tif x.ndim != 1: raise ValueError, "smooth only accepts 1 dimension arrays." \\tif x.size < window_len: return x \\tif window_len<3: return x \\tif not window in ['flat', 'hanning', 'hamming', 'bartlett', 'blackman']: raise ValueError, "Window is on of 'flat', 'hanning', 'hamming', 'bartlett', 'blackman'" \\ts = numpy.r_[x[window_len-1:0:-1],x,x[-1:-window_len:-1]] \\tif window == 'flat': # moving average \\t\\tw = numpy.ones(window_len,'d') \\telse: \\t\\tw = eval('numpy.'+window+'(window_len)') \\ty = numpy.convolve(w/w.sum(),s,mode='valid') \\treturn y[(window_len/2-1):-(window_len/2)] ]; $$opts{plt_fh} = $fh; } sub percentile { my ($p,@vals) = @_; my $N = 0; for my $val (@vals) { $N += $val; } my $n = $p*($N+1)/100.; my $k = int($n); my $d = $n-$k; if ( $k<=0 ) { return 0; } if ( $k>=$N ) { return scalar @vals-1; } my $cnt; for (my $i=0; $i<@vals; $i++) { $cnt += $vals[$i]; if ( $cnt>=$k ) { return $i; } } error("FIXME: this should not happen [percentile]\n"); } sub get_values { my ($opts,$id,$key,$i,$j) = @_; if ( !exists($$opts{dat}{$key}) ) { return (); } if ( !exists($$opts{dat}{$key}{$id}) ) { return (); } my $fields_ok = 1; if ( !exists($$opts{exp}{$key}) ) { error("todo: sanity check for $key\n"); } if ( exists($$opts{def_line}{$key}) && $$opts{def_line}{$key} ne $$opts{exp}{$key} && !$$opts{def_line_warned}{$key} ) { warn("Warning: Possible version mismatch, the definition line differs\n\texpected: $$opts{exp}{$key}\n\tfound: $$opts{def_line}{$key}\n"); $$opts{def_line_warned}{$key} = 1; } if ( defined $i ) { if ( defined $j ) { return $$opts{dat}{$key}{$id}[$i][$j]; } return (@{$$opts{dat}{$key}{$id}[$i]}); } return (@{$$opts{dat}{$key}{$id}}); } sub get_value { my ($opts,$id,$key) = @_; if ( !exists($$opts{dat}{$id}) ) { return undef; } if ( !exists($$opts{dat}{$id}{$key}) ) { return undef} return $$opts{dat}{$id}{$key}; } sub plot_venn_bars { my ($opts) = @_; my @ids = file_ids($opts); if ( @ids != 3 ) { return; } my (@snps,@indels,@tstv,@snp_titles,@indel_titles); for my $id (0..2) { push @snps, get_value($opts,$id,'number of SNPs:'); push @indels, get_value($opts,$id,'number of indels:'); push @tstv, sprintf("%.2f",get_values($opts,$id,'TSTV',0,5)); push @snp_titles, "$$opts{title}{$id}\\nts/tv $tstv[$id]\\n" .bignum($snps[$id]); my @fs = get_values($opts,$id,'FS'); my $fs = @fs ? "frm $fs[0][3]\\n" : ''; push @indel_titles, "$$opts{title}{$id}\\n$fs" .bignum($indels[$id]); } my $fh = $$opts{plt_fh}; tprint $fh, qq[ if plot_venn_snps: \\tfig = plt.figure(figsize=($$opts{img_width},$$opts{img_height})) \\tax1 = fig.add_subplot(111) \\tax1.bar([1,2,3],[$snps[0],$snps[2],$snps[1]],align='center',color='$$opts{id2col}[0]',width=0.3) \\tax1.ticklabel_format(style='sci', scilimits=(0,0), axis='y') \\tax1.set_xlim(0.5,3.5) \\tplt.xticks([1,2,3],('$snp_titles[0]','$snp_titles[2]','$snp_titles[1]')) \\tplt.title('Number of SNPs') \\tplt.subplots_adjust(right=0.95,bottom=0.15) \\tplt.savefig('$$opts{prefix}venn_bars.snps.png') \\tif pdf_plots: plt.savefig('$$opts{prefix}venn_bars.snps.pdf') \\tplt.close() if plot_venn_indels: \\tfig = plt.figure(figsize=($$opts{img_width},$$opts{img_height})) \\tax1 = fig.add_subplot(111) \\tax1.bar([1,2,3],[$indels[0],$indels[2],$indels[1]],align='center',color='$$opts{id2col}[1]',width=0.3) \\tax1.ticklabel_format(style='sci', scilimits=(0,0), axis='y') \\tax1.set_xlim(0.5,3.5) \\tplt.xticks([1,2,3],('$indel_titles[0]','$indel_titles[2]','$indel_titles[1]')) \\tplt.title('Number of indels') \\tplt.subplots_adjust(right=0.95,bottom=0.15) \\tplt.savefig('$$opts{prefix}venn_bars.indels.png') \\tif pdf_plots: plt.savefig('$$opts{prefix}venn_bars.indels.pdf') \\tplt.close() ]; } sub plot_per_sample_stats { my ($opts,$id) = @_; my @vals = get_values($opts,$id,'PSC'); if ( !@vals ) { return; } my $fh = $$opts{plt_fh}; my $img = "$$opts{prefix}tstv_by_sample.$id"; my $img2 = "$$opts{prefix}hets_by_sample.$id"; my $img3 = "$$opts{prefix}snps_by_sample.$id"; my $img4 = "$$opts{prefix}indels_by_sample.$id"; my $img5 = "$$opts{prefix}singletons_by_sample.$id"; my $img6 = "$$opts{prefix}dp_by_sample.$id"; open(my $tfh,'>',"$img.dat") or error("$img.dat: $!"); print $tfh "# [1]Sample ID\t[2]ts/tv\t[3]het/hom\t[4]nSNPs\t[5]nIndels\t[6]Average depth\t[7]nSingletons\t[8]Sample name\n"; for (my $i=0; $i<@vals; $i++) { my $tstv = $vals[$i][5] ? $vals[$i][4]/$vals[$i][5] : 0; my $hethom = $vals[$i][2] ? $vals[$i][3]/$vals[$i][2] : 0; printf $tfh "%d\t%f\t%f\t%d\t%d\t%f\t%d\t%s\n", $i, $tstv, $hethom, $vals[$i][4]+$vals[$i][5], $vals[$i][6], $vals[$i][7], $vals[$i][8], $vals[$i][0]; } close($tfh); tprint $fh, " dat = [] with open('$img.dat', 'rb') as f: \\treader = csv.reader(f, 'tab') \\tfor row in reader: \\t\\tif row[0][0] != '#': dat.append(row) if plot_tstv_by_sample: \\tfig = plt.figure(figsize=(2*$$opts{img_width},$$opts{img_height}*0.7)) \\tax1 = fig.add_subplot(111) \\tax1.plot([row[0] for row in dat], [row[1] for row in dat], 'o', color='$$opts{id2col}[$id]',mec='$$opts{id2col}[$id]') \\tax1.set_ylabel('Ts/Tv') \\tax1.set_ylim(min(float(row[1]) for row in dat)-0.1,max(float(row[1]) for row in dat)+0.1) \\tif sample_names: \\t\\t plt.xticks([row[0] for row in dat],[row[7] for row in dat],**sample_font) \\t\\t plt.subplots_adjust(**sample_margins) \\telse: \\t\\t plt.subplots_adjust(right=0.98,left=0.07,bottom=0.17) \\t\\t ax1.set_xlabel('Sample ID') \\tplt.title('$$opts{title}{$id}') \\tplt.savefig('$img.png') \\tif pdf_plots: plt.savefig('$img.pdf') \\tplt.close() if plot_hethom_by_sample: \\tfig = plt.figure(figsize=(2*$$opts{img_width},$$opts{img_height}*0.7)) \\tax1 = fig.add_subplot(111) \\tax1.plot([row[0] for row in dat], [row[2] for row in dat], 'o', color='$$opts{id2col}[$id]',mec='$$opts{id2col}[$id]') \\tax1.set_ylabel('nHet(RA) / nHom(AA)') \\tax1.ticklabel_format(style='sci', scilimits=(0,0), axis='y') \\tif sample_names: \\t\\t plt.xticks([row[0] for row in dat],[row[7] for row in dat],**sample_font) \\t\\t plt.subplots_adjust(**sample_margins) \\telse: \\t\\t plt.subplots_adjust(right=0.98,left=0.07,bottom=0.17) \\t\\t ax1.set_xlabel('Sample ID') \\tplt.title('$$opts{title}{$id}') \\tplt.savefig('$img2.png') \\tif pdf_plots: plt.savefig('$img2.pdf') \\tplt.close() if plot_snps_by_sample: \\tfig = plt.figure(figsize=(2*$$opts{img_width},$$opts{img_height}*0.7)) \\tax1 = fig.add_subplot(111) \\tax1.plot([row[0] for row in dat], [row[3] for row in dat], 'o', color='$$opts{id2col}[$id]',mec='$$opts{id2col}[$id]') \\tax1.set_ylabel('Number of SNPs') \\tax1.ticklabel_format(style='sci', scilimits=(0,0), axis='y') \\tif sample_names: \\t\\t plt.xticks([row[0] for row in dat],[row[7] for row in dat],**sample_font) \\t\\t plt.subplots_adjust(**sample_margins) \\telse: \\t\\t plt.subplots_adjust(right=0.98,left=0.07,bottom=0.17) \\t\\t ax1.set_xlabel('Sample ID') \\tplt.title('$$opts{title}{$id}') \\tplt.savefig('$img3.png') \\tif pdf_plots: plt.savefig('$img3.pdf') \\tplt.close() if plot_indels_by_sample: \\tfig = plt.figure(figsize=(2*$$opts{img_width},$$opts{img_height}*0.7)) \\tax1 = fig.add_subplot(111) \\tax1.plot([row[0] for row in dat], [row[4] for row in dat], 'o', color='$$opts{id2col}[$id]',mec='$$opts{id2col}[$id]') \\tax1.set_ylabel('Number of indels') \\tax1.ticklabel_format(style='sci', scilimits=(0,0), axis='y') \\tif sample_names: \\t\\t plt.xticks([row[0] for row in dat],[row[7] for row in dat],**sample_font) \\t\\t plt.subplots_adjust(**sample_margins) \\telse: \\t\\t plt.subplots_adjust(right=0.98,left=0.07,bottom=0.17) \\t\\t ax1.set_xlabel('Sample ID') \\tplt.title('$$opts{title}{$id}') \\tplt.savefig('$img4.png') \\tif pdf_plots: plt.savefig('$img4.pdf') \\tplt.close() if plot_singletons_by_sample: \\tfig = plt.figure(figsize=(2*$$opts{img_width},$$opts{img_height}*0.7)) \\tax1 = fig.add_subplot(111) \\tax1.plot([row[0] for row in dat], [row[6] for row in dat], 'o', color='$$opts{id2col}[$id]',mec='$$opts{id2col}[$id]') \\tax1.set_ylabel('Number of singletons') \\tax1.ticklabel_format(style='sci', scilimits=(0,0), axis='y') \\tif sample_names: \\t\\t plt.xticks([row[0] for row in dat],[row[7] for row in dat],**sample_font) \\t\\t plt.subplots_adjust(**sample_margins) \\telse: \\t\\t plt.subplots_adjust(right=0.98,left=0.07,bottom=0.17) \\t\\t ax1.set_xlabel('Sample ID') \\tplt.title('$$opts{title}{$id}') \\tplt.savefig('$img5.png') \\tif pdf_plots: plt.savefig('$img5.pdf') \\tplt.close() if plot_depth_by_sample: \\tfig = plt.figure(figsize=(2*$$opts{img_width},$$opts{img_height}*0.7)) \\tax1 = fig.add_subplot(111) \\tax1.plot([row[0] for row in dat], [row[5] for row in dat], 'o', color='$$opts{id2col}[$id]',mec='$$opts{id2col}[$id]') \\tax1.set_ylabel('Average depth') \\tax1.ticklabel_format(style='sci', scilimits=(0,0), axis='y') \\tif sample_names: \\t\\t plt.xticks([row[0] for row in dat],[row[7] for row in dat],**sample_font) \\t\\t plt.subplots_adjust(**sample_margins) \\telse: \\t\\t plt.subplots_adjust(right=0.98,left=0.07,bottom=0.17) \\t\\t ax1.set_xlabel('Sample ID') \\tplt.title('$$opts{title}{$id}') \\tplt.savefig('$img6.png') \\tif pdf_plots: plt.savefig('$img6.pdf') \\tplt.close() "; } sub plot_DP { my ($opts,$id) = @_; my @vals = get_values($opts,$id,'DP'); if ( !@vals ) { return; } my $fh = $$opts{plt_fh}; my $img = "$$opts{prefix}depth.$id"; open(my $tfh,'>',"$img.dat") or error("$img.dat: $!"); print $tfh "# [1]Depth\t[2]Cumulative number of genotypes\t[3]Number of genotypes\n"; my $sum = 0; for (my $i=0; $i<@vals; $i++) { if ( $sum>99. ) { last; } if ( !($vals[$i][0]=~/^\d+$/) ) { next; } # DP ">500" case $sum += $vals[$i][2]; printf $tfh "%d\t%f\t%f\n", $vals[$i][0], $sum, $vals[$i][2]; } close($tfh); tprint $fh, " dat = [] with open('$img.dat', 'rb') as f: \\treader = csv.reader(f, 'tab') \\tfor row in reader: \\t\\tif row[0][0] != '#': dat.append(row) if plot_dp_dist: \\tfig = plt.figure(figsize=($$opts{img_width},$$opts{img_height})) \\tax1 = fig.add_subplot(111) \\tax1.plot([row[0] for row in dat], [row[2] for row in dat], '-^', color='k') \\tax1.set_ylabel('Number of genotypes [%]',color='k') \\tax1.set_xlabel('Depth') \\tax2 = ax1.twinx() \\tax2.plot([row[0] for row in dat], [row[1] for row in dat], '-o', color='$$opts{id2col}[$id]') \\tax2.set_ylabel('Cumulative number of genotypes [%]',color='$$opts{id2col}[$id]') \\tfor tl in ax2.get_yticklabels(): tl.set_color('$$opts{id2col}[$id]') \\tplt.subplots_adjust(left=0.15,bottom=0.15,right=0.87) \\tplt.title('$$opts{title}{$id}') \\tplt.savefig('$img.png') \\tif pdf_plots: plt.savefig('$img.pdf') \\tplt.close() "; } sub plot_hwe { my ($opts,$id) = @_; my @vals = get_values($opts,$id,'HWE'); if ( !@vals ) { return; } my $fh = $$opts{plt_fh}; my $img = "$$opts{prefix}hwe.$id"; open(my $tfh,'>',"$img.dat") or error("$img.dat: $!"); print $tfh "# [1]Allele Frequency\t[2]Depth\t[3]Number of hets (median)\t[4]Number of hets (25-75th percentile)\n"; for (my $i=0; $i<@vals; $i++) { if ( !$vals[$i][1] ) { next; } print $tfh join("\t", @{$vals[$i]}), "\n"; } close($tfh); tprint $fh, " dat = [] with open('$img.dat', 'rb') as f: \\treader = csv.reader(f, 'tab') \\tfor row in reader: \\t\\tif row[0][0] != '#': dat.append(row) if plot_hwe and len(dat)>1: \\tx = [float(row[0]) for row in dat] \\ty1 = smooth(numpy.array([float(row[2]) for row in dat]),40,'hanning') \\ty2 = smooth(numpy.array([float(row[3]) for row in dat]),40,'hanning') \\ty3 = smooth(numpy.array([float(row[4]) for row in dat]),40,'hanning') \\tdp = smooth(numpy.array([float(row[1]) for row in dat]),40,'hanning') \\thwe = [] \\tfor af in x: hwe.append(2*af/100.*(1-af/100.)) \\tfig = plt.figure(figsize=($$opts{img_width},$$opts{img_height})) \\tax1 = fig.add_subplot(111) \\tplots = ax1.plot(x,hwe,'--',color='#ff9900',label='HWE') \\tplots += ax1.plot(x,y2,color='#ff9900',label='Median') \\tplots += ax1.plot(x,y3,color='#ffe0b2',label='25-75th percentile') \\tax1.fill_between(x,y1,y3, facecolor='#ffeacc',edgecolor='#ffe0b2') \\tax1.set_ylabel('Fraction of hets',color='#ff9900') \\tax1.set_xlabel('Allele frequency [%]') \\tfor tl in ax1.get_yticklabels(): tl.set_color('#ff9900') \\tax2 = ax1.twinx() \\tplots += ax2.plot(x,dp, 'k', label='Number of sites') \\tax2.set_ylabel('Number of sites') \\tax2.set_yscale('log') \\tlabels = [l.get_label() for l in plots] \\tplt.legend(plots,labels,numpoints=1,markerscale=2,loc='center',prop={'size':9},frameon=False) \\tplt.subplots_adjust(left=0.15,bottom=0.15,right=0.86) \\tplt.title('$$opts{title}{$id}') \\tplt.savefig('$img.png') \\tif pdf_plots: plt.savefig('$img.pdf') \\tplt.close() "; } sub plot_irc_by_rlen { my ($opts,$id) = @_; my @vals = get_values($opts,$id,'ICL'); if ( !@vals ) { return; } my $fh = $$opts{plt_fh}; my $img = "$$opts{prefix}irc_by_rlen.$id"; open(my $tfh,'>',"$img.dat") or error("$img.dat: $!"); print $tfh "# [1]Repeat type\t[2]Total count\t[3]repeat consistency: deletions\t[4]repeat consistency: insertions\n"; for (my $i=0; $i<@vals; $i++) { printf $tfh "%d\t%d\t%f\t%f\n", $vals[$i][0], # repeat type $vals[$i][1]+$vals[$i][2]+$vals[$i][3]+$vals[$i][4], # total count $vals[$i][2] ? $vals[$i][1]/($vals[$i][1]+$vals[$i][2]) : 0, # IRC of deletions $vals[$i][4] ? $vals[$i][3]/($vals[$i][3]+$vals[$i][4]) : 0 # IRC of insertions ; } close($tfh); tprint $fh, " dat = [] with open('$img.dat', 'rb') as f: \\treader = csv.reader(f, 'tab') \\tfor row in reader: \\t\\tif row[0][0] != '#': dat.append([float(x) for x in row]) if plot_irc_by_len and len(dat): \\tfig = plt.figure(figsize=($$opts{img_width},$$opts{img_height})) \\tax1 = fig.add_subplot(111) \\tax2 = ax1.twinx() \\tplots = ax1.plot([row[0] for row in dat], [row[2] for row in dat], 'o-', ms=3, color='r', mec='r', label='Deletions'); \\tplots += ax1.plot([row[0] for row in dat], [row[3] for row in dat], 'o-', ms=3, color='g', mec='g', label='Insertions'); \\tplots += ax2.plot([row[0] for row in dat], [row[1] for row in dat], '^--', ms=3, color='k', mec='k', label='Number of sites'); \\tax2.ticklabel_format(style='sci',scilimits=(-3,4)) \\tax1.set_xlabel('Repeat type'); \\tax1.set_ylabel('Repeat Consistency'); \\tax2.set_ylabel('Number of sites'); \\tlabels = [l.get_label() for l in plots] \\tax1.legend(plots,labels,numpoints=1,markerscale=1,loc='best',prop={'size':9},frameon=False) \\tplt.subplots_adjust(right=0.88,left=0.15,bottom=0.11) \\tplt.title('$$opts{title}{$id}') \\tplt.savefig('$img.png') \\tif pdf_plots: plt.savefig('$img.pdf') \\tplt.close() "; } sub plot_irc_by_AF { error("plot_irc_by_AF: deprecated\n"); my ($opts,$id) = @_; my @vals = get_values($opts,$id,'AF'); if ( !@vals ) { return; } my $fh = $$opts{plt_fh}; my $img = "$$opts{prefix}irc_by_af.$id"; my $vals = rebin_values(\@vals,8,0); open(my $tfh,'>',"$img.dat") or error("$img.dat: $!"); print $tfh "# [1]Repeat type\t[2]Total count\t[3]repeat consistency: deletions\t[4]repeat consistency: insertions\n"; my $has_vals = 0; tprint $fh, " dat = [ "; for (my $i=0; $i<@$vals; $i++) { if ( $$vals[$i][5] + $$vals[$i][6] == 0 ) { next; } $has_vals++; tprint $fh, sprintf("\t[ %f, %d, %f, %f ],\n", $$vals[$i][0]/100., $$vals[$i][5] + $$vals[$i][6], $$vals[$i][6] ? $$vals[$i][5]/($$vals[$i][5] + $$vals[$i][6]) : 0, ($$vals[$i][6] + $$vals[$i][7]) ? $$vals[$i][5]/($$vals[$i][5] + $$vals[$i][6] + $$vals[$i][7]) : 0); } tprint $fh, "]"; if ( $has_vals<2 ) { return; } tprint $fh, " fig = plt.figure(figsize=($$opts{img_width},$$opts{img_height})) ax1 = fig.add_subplot(111) ax1.plot([row[0] for row in dat], [row[1] for row in dat], '-o',color='k',mec='k',markersize=3) ax1.set_ylabel('Number of sites',color='k') ax1.set_yscale('log') #ax1.ticklabel_format(style='sci', scilimits=(0,0), axis='y') for tl in ax1.get_yticklabels(): tl.set_color('k') ax1.set_xlabel('Non-ref allele frequency') ax2 = ax1.twinx() ax2.plot([row[0] for row in dat], [row[2] for row in dat], '-o',color='$$opts{id2col}[$id]',mec='$$opts{id2col}[$id]',markersize=3,label='c/(c+i)') ax2.plot([row[0] for row in dat], [row[3] for row in dat], '--^',color='$$opts{id2col}[$id]',mec='$$opts{id2col}[$id]',markersize=3,label='c/(c+i+n/a)') ax2.legend(numpoints=1,markerscale=2,loc='best',prop={'size':10},frameon=False) ax2.set_ylabel('Indel Repeat Consistency',color='$$opts{id2col}[$id]') ax2.set_ylim(0,1) ax1.set_xlim(0,1) for tl in ax2.get_yticklabels(): tl.set_color('$$opts{id2col}[$id]') plt.subplots_adjust(right=0.88,left=0.15,bottom=0.11) plt.title('$$opts{title}{$id}') plt.savefig('$img.png') if pdf_plots: plt.savefig('$img.pdf') plt.close() "; } sub plot_tstv_by_AF { my ($opts,$id) = @_; my @vals = get_values($opts,$id,'AF'); if ( !@vals ) { return; } my $fh = $$opts{plt_fh}; my $img = "$$opts{prefix}tstv_by_af.$id"; my $vals = rebin_values(\@vals,8,0); open(my $tfh,'>',"$img.dat") or error("$img.dat: $!"); print $tfh "# [1]Allele frequency\t[2]Number of sites\t[3]ts/tv\n"; for (my $i=0; $i<@$vals; $i++) { if ( $$vals[$i][2] + $$vals[$i][3] == 0 ) { next; } printf $tfh "%f\t%d\t%f\n", $$vals[$i][0]/100., $$vals[$i][2] + $$vals[$i][3], $$vals[$i][3] ? $$vals[$i][2]/$$vals[$i][3]: 0; } close($tfh); tprint $fh, " dat = [] with open('$img.dat', 'rb') as f: \\treader = csv.reader(f, 'tab') \\tfor row in reader: \\t\\tif row[0][0] != '#': dat.append([float(x) for x in row]) if plot_tstv_by_af and len(dat)>2: \\tfig = plt.figure(figsize=($$opts{img_width},$$opts{img_height})) \\tax1 = fig.add_subplot(111) \\tax1.plot([row[0] for row in dat], [row[1] for row in dat], '-o',color='k',mec='k',markersize=3) \\tax1.set_ylabel('Number of sites',color='k') \\tax1.set_yscale('log') \\t#ax1.ticklabel_format(style='sci', scilimits=(0,0), axis='y') \\tfor tl in ax1.get_yticklabels(): tl.set_color('k') \\tax1.set_xlabel('Non-ref allele frequency') \\tax2 = ax1.twinx() \\tax2.plot([row[0] for row in dat], [row[2] for row in dat], '-o',color='$$opts{id2col}[$id]',mec='$$opts{id2col}[$id]',markersize=3) \\tax2.set_ylabel('Ts/Tv',color='$$opts{id2col}[$id]') \\tax2.set_ylim(0,0.5+max(3,max(row[2] for row in dat))) \\tax1.set_xlim(0,1) \\tfor tl in ax2.get_yticklabels(): tl.set_color('$$opts{id2col}[$id]') \\tplt.subplots_adjust(right=0.88,left=0.15,bottom=0.11) \\tplt.title('$$opts{title}{$id}') \\tplt.savefig('$img.png') \\tif pdf_plots: plt.savefig('$img.pdf') \\tplt.close() "; } sub plot_tstv_by_QUAL { my ($opts,$id) = @_; my @vals = get_values($opts,$id,'QUAL'); if ( !@vals ) { return; } my $fh = $$opts{plt_fh}; my $img = "$$opts{prefix}tstv_by_qual.$id"; open(my $tfh,'>',"$img.dat") or error("$img.dat: $!"); print $tfh "# [1]Quality\t[2]Number of sites\t[3]Marginal Ts/Tv\n"; my @dat = (); my $ntot = 0; for my $val (@vals) { push @dat, [ $$val[0], $$val[2], $$val[3] ]; # qual, nts, ntv $ntot += $$val[2] + $$val[3]; } my @sdat = sort { $$b[0] <=> $$a[0] } @dat; push @sdat, [-1]; my $dn = $ntot*0.05; my $qprev = $sdat[0][0]; my $nts = 0; my $ntv = 0; my $nout = 0; for my $rec (@sdat) { if ( $$rec[0]==-1 or $nts+$ntv > $dn ) { if ( $ntv ) { printf $tfh "$qprev\t%d\t%f\n", $nts+$ntv+$nout,$nts/$ntv; } if ( $$rec[0]==-1 ) { last; } $nout += $nts+$ntv; $nts = 0; $ntv = 0; $qprev = $$rec[0]; } $nts += $$rec[1]; $ntv += $$rec[2]; } close($tfh) or error("close $img.dat"); tprint $fh, " dat = [] with open('$img.dat', 'rb') as f: \\treader = csv.reader(f, 'tab') \\tfor row in reader: \\t\\tif row[0][0] != '#': dat.append([float(x) for x in row]) if plot_tstv_by_qual: \\tfig = plt.figure(figsize=($$opts{img_width},$$opts{img_height})) \\tax1 = fig.add_subplot(111) \\tax1.plot([row[1] for row in dat], [row[2] for row in dat], '^-', ms=3, mec='$$opts{id2col}[$id]', color='$$opts{id2col}[$id]') \\tax1.set_ylabel('Ts/Tv',fontsize=10) \\tax1.set_xlabel('Number of sites\\n(sorted by QUAL, descending)',fontsize=10) \\tax1.ticklabel_format(style='sci', scilimits=(-3,2), axis='x') \\tax1.set_ylim(min(2,min(row[2] for row in dat))-0.3,0.3+max(2.2,max(row[2] for row in dat))) \\tplt.subplots_adjust(right=0.88,left=0.15,bottom=0.15) \\tplt.title('$$opts{title}{$id}') \\tplt.savefig('$img.png') \\tif pdf_plots: plt.savefig('$img.pdf') \\tplt.close() "; } sub rebin_values { my ($vals,$bin_size,$col,%args) = @_; my %avg = exists($args{avg}) ? map {$_=>1} @{$args{avg}} : (); my $prev = $$vals[0][$col]; my $iout = 0; my $nsum = 0; my (@dat,@out); for (my $i=0; $i<@$vals; $i++) { for (my $icol=0; $icol<@{$$vals[$i]}; $icol++) { if ( $icol==$col ) { next; } $dat[$icol] += $$vals[$i][$icol]; } $nsum++; if ( $i+1<@$vals && $$vals[$i][$col] - $prev < $bin_size ) { next; } $dat[$col] = $prev; for (my $icol=0; $icol<@{$$vals[$i]}; $icol++) { $out[$iout][$icol] = $dat[$icol] ? $dat[$icol] : 0; if ( $avg{$icol} && $nsum ) { $out[$iout][$icol] /= $nsum; } } $nsum = 0; @dat = (); $iout++; $prev = $$vals[$i][$col]; } return \@out; } sub plot_concordance_by_AF { my ($opts) = @_; my @vals = get_values($opts,2,'GCsAF'); if ( !@vals ) { return; } # create a local copy and prepare r2 for rebinning @vals = @{ dclone(\@vals) }; for (my $i=0; $i<@vals; $i++) { $vals[$i][7] *= $vals[$i][8]; } my $vals = rebin_values(\@vals,1,0); my $fh = $$opts{plt_fh}; my $img = "$$opts{prefix}gts_by_af"; my $img2 = "$$opts{prefix}r2_by_af"; open(my $tfh,'>',"$img.dat") or error("$img.dat: $!"); print $tfh "# [1]Allele Frequency\t[2]RR concordance\t[3]RA concordance\t[4]AA concordance\t[5]nRR\t[6]nRA\t[7]nAA\t[8]R^2\t[9]Number of sites\n"; for (my $i=0; $i<@$vals; $i++) { printf $tfh "%f\t%f\t%f\t%f\t%d\t%d\t%d\t%f\t%d\n", $$vals[$i][0]/100., $$vals[$i][1]+$$vals[$i][4] ? $$vals[$i][1]/($$vals[$i][1]+$$vals[$i][4]) : 1, $$vals[$i][2]+$$vals[$i][5] ? $$vals[$i][2]/($$vals[$i][2]+$$vals[$i][5]) : 1, $$vals[$i][3]+$$vals[$i][6] ? $$vals[$i][3]/($$vals[$i][3]+$$vals[$i][6]) : 1, $$vals[$i][1]+$$vals[$i][4], $$vals[$i][2]+$$vals[$i][5], $$vals[$i][3]+$$vals[$i][6], $$vals[$i][8] ? $$vals[$i][7]/$$vals[$i][8] : 1, $$vals[$i][8]; } close($tfh); tprint $fh, " dat = [] with open('$img.dat', 'rb') as f: \\treader = csv.reader(f, 'tab') \\tfor row in reader: \\t\\tif row[0][0] != '#': dat.append(row) if plot_concordance_by_af and len(dat)>1: \\tfig = plt.figure(figsize=($$opts{img_width}*1.2,$$opts{img_height})) \\tax1 = fig.add_subplot(111) \\tax1.plot([row[0] for row in dat], [row[1] for row in dat],'.',color='$$opts{id2col}[1]',label='Hom RR') \\tax1.plot([row[0] for row in dat], [row[2] for row in dat],'.',color='$$opts{id2col}[0]',label='Het RA') \\tax1.plot([row[0] for row in dat], [row[3] for row in dat],'.',color='k',label='Hom AA') \\tax1.set_xlabel('Non-ref allele frequency') \\tax1.set_ylabel('Concordance') \\tleg = ax1.legend(title='Concordance:',numpoints=1,markerscale=2,loc='best',prop={'size':9}) \\tleg.draw_frame(False) \\tplt.setp(leg.get_title(),fontsize=9) \\tax2 = ax1.twinx() \\tax2.plot([row[0] for row in dat], [row[4] for row in dat],color='$$opts{id2col}[1]') \\tax2.plot([row[0] for row in dat], [row[5] for row in dat],color='$$opts{id2col}[0]') \\tax2.plot([row[0] for row in dat], [row[6] for row in dat],color='k') \\tax2.set_ylabel('Number of genotypes') \\tax2.set_yscale('log') \\tplt.subplots_adjust(left=0.15,right=0.83,bottom=0.11) \\tplt.savefig('$img.png') \\tif pdf_plots: plt.savefig('$img.pdf') \\tplt.close() if plot_r2_by_af and len(dat)>1: \\tfig = plt.figure(figsize=($$opts{img_width}*1.3,$$opts{img_height})) \\tax1 = fig.add_subplot(111) \\tax2 = ax1.twinx() \\tax1.set_zorder(ax2.get_zorder()+1) \\tax1.patch.set_visible(False) \\tax2.plot([row[0] for row in dat], [row[8] for row in dat], '-o', color='r',mec='r',markersize=3) \\tax1.plot([row[0] for row in dat], [row[7] for row in dat], '-^', color='k',markersize=3) \\tfor tl in ax2.get_yticklabels(): tl.set_color('r') \\tax2.set_ylabel('Number of sites', color='r') \\tax2.set_yscale('log') \\tax1.set_ylabel('Mean allelic R\$^2\$', color='k') \\tax1.set_xlabel('Non-ref allele frequency') \\tplt.subplots_adjust(left=0.19,right=0.83,bottom=0.11) \\tplt.savefig('$img2.png') \\tif pdf_plots: plt.savefig('$img2.pdf') \\tplt.close() "; } sub plot_concordance_by_sample { my ($opts) = @_; my @vals = get_values($opts,2,'GCsS'); if ( !@vals ) { return; } my $fh = $$opts{plt_fh}; my $img = "$$opts{prefix}gts_by_sample"; open(my $tfh,'>',"$img.dat") or error("$img.dat: $!"); print $tfh "# [1]Sample ID\t[2]Discordance\t[3]Sample Name\n"; for (my $i=0; $i<@vals; $i++) { printf $tfh "%d\t%f\t%s\n", $i, $vals[$i][1], $vals[$i][0]; } close($tfh); tprint $fh, " dat = [] with open('$img.dat', 'rb') as f: \\treader = csv.reader(f, 'tab') \\tfor row in reader: \\t\\tif row[0][0] != '#': dat.append(row) if plot_discordance_by_sample: \\tfig = plt.figure(figsize=(2*$$opts{img_width},$$opts{img_height}*0.7)) \\tax1 = fig.add_subplot(111) \\tax1.plot([row[0] for row in dat], [row[1] for row in dat],'.',color='orange') \\tax1.set_ylabel('Non-ref discordance') \\tax1.set_ylim(0,) \\tif sample_names: \\t\\t plt.xticks([row[0] for row in dat],[row[2] for row in dat],**sample_font) \\t\\t plt.subplots_adjust(**sample_margins) \\telse: \\t\\t plt.subplots_adjust(right=0.98,left=0.07,bottom=0.17) \\t\\t ax1.set_xlabel('Sample ID') \\tplt.savefig('$img.png') \\tif pdf_plots: plt.savefig('$img.pdf') \\tplt.close() "; } sub plot_counts_by_AF { my ($opts) = @_; plot_counts_by_AF_col($opts,1,'SNP'); plot_counts_by_AF_col($opts,4,'Indel'); } sub plot_counts_by_AF_col { my ($opts,$col,$title) = @_; my $fh = $$opts{plt_fh}; my $img = "$$opts{prefix}counts_by_af.".lc($title)."s"; open(my $tfh,'>',"$img.dat") or error("$img.dat: $!"); print $tfh "# [1]id\t[2]Nonref Allele Frequency\t[3]Number of sites\n"; for my $id (file_ids($opts)) { my @tmp = get_values($opts,$id,'AF'); my $vals = rebin_values(\@tmp,1,0); for my $val (@$vals) { if ( !$$val[$col] ) { next; } print $tfh "$id\t$$val[0]\t$$val[$col]\n"; } } close($tfh); tprint $fh, " dat = {} with open('$img.dat', 'rb') as f: \\treader = csv.reader(f, 'tab') \\tfor row in reader: \\t\\tif row[0][0] == '#': continue \\t\\tid = int(row[0]) \\t\\tif id not in dat: dat[id] = [] \\t\\tdat[id].append([float(row[1]),float(row[2])]) if plot_${title}_count_by_af: \\tfig = plt.figure(figsize=(2*$$opts{img_width},$$opts{img_height}*0.7)) \\tax1 = fig.add_subplot(111) \\tax1.set_ylabel('Number of sites') \\tax1.ticklabel_format(style='sci', scilimits=(0,0), axis='y') \\tax1.set_yscale('log') \\tax1.set_xlabel('Non-reference allele frequency') \\tax1.set_xlim(-0.05,1.05) \\thas_data = 0 "; for my $id (file_ids($opts)) { tprint $fh, " \\tif $id in dat and len(dat[$id])>2: \\t\\tax1.plot([row[0]/100. for row in dat[$id]], [row[1] for row in dat[$id]], '-o',markersize=3, color='$$opts{id2col}[$id]',mec='$$opts{id2col}[$id]',label='$$opts{title}{$id}') \\t\\thas_data = 1 "; } tprint $fh, " \\tif has_data: \\t\\tax1.legend(numpoints=1,markerscale=1,loc='best',prop={'size':10},frameon=False) \\t\\tplt.title('$title count by AF') \\t\\tplt.subplots_adjust(bottom=0.2,left=0.1,right=0.95) \\t\\tplt.savefig('$img.png') \\t\\tif pdf_plots: plt.savefig('$img.pdf') \\t\\tplt.close() "; } sub plot_overlap_by_AF { my ($opts) = @_; plot_overlap_by_AF_col($opts,1,'SNP'); plot_overlap_by_AF_col($opts,4,'Indel'); } sub plot_overlap_by_AF_col { my ($opts,$col,$title) = @_; my @ids = file_ids($opts); if ( @ids != 3 ) { return; } my ($ia,$ib,$iab); for (my $i=0; $i<@ids; $i++) { if ( @{$$opts{dat}{ID}{$ids[$i]}[0]}>1 ) { $iab = $i; next; } if ( !defined $ia ) { $ia = $i; next; } $ib = $i; } my $fh = $$opts{plt_fh}; my $img = "$$opts{prefix}overlap_by_af.".lc($title)."s"; my @has_vals; my @vals_a = get_values($opts,$ia,'AF'); my @vals_b = get_values($opts,$ib,'AF'); my @vals_ab = get_values($opts,$iab,'AF'); my (%afs,%af_a,%af_ab); for my $val (@vals_a) { $afs{$$val[0]} = $$val[$col]; $af_a{$$val[0]} = $$val[$col]; } for my $val (@vals_ab) { $afs{$$val[0]} = $$val[$col]; $af_ab{$$val[0]} = $$val[$col]; } open(my $tfh,'>',"$img.dat") or error("$img.dat: $!"); print $tfh "# [1]Allele frequency\t[2]Fraction of sites from $$opts{title}{$ids[$ia]} also in $$opts{title}{$ids[$ib]}\t[3]Number of sites\n"; for my $af (sort { $a<=>$b } keys %afs) { my $a = exists($af_a{$af}) ? $af_a{$af} : 0; my $ab = exists($af_ab{$af}) ? $af_ab{$af} : 0; my $yval = ($a+$ab) ? $ab * 100. / ($a + $ab) : 0; print $tfh "$af\t$yval\t" .($a+$ab). "\n"; } close($tfh) or error("close $img.dat"); tprint $fh, " dat = [] with open('$img.dat', 'rb') as f: \\treader = csv.reader(f, 'tab') \\tfor row in reader: \\t\\tif row[0][0] != '#': dat.append(row) if plot_${title}_overlap_by_af and len(dat)>1: \\tfig = plt.figure(figsize=(2*$$opts{img_width},$$opts{img_height}*0.7)) \\tax1 = fig.add_subplot(111) \\tax1.plot([row[0] for row in dat], [row[1] for row in dat],'-o',markersize=3, color='$$opts{id2col}[1]',mec='$$opts{id2col}[1]') \\tax1.set_ylabel('Fraction found in $$opts{title}{$ib} [%]') \\tax1.set_xscale('log') \\tax1.set_xlabel('Non-reference allele frequency in $$opts{title}{$ia} [%]') \\tax1.set_xlim(0,11) \\tax1.set_xticks([0.1,0.2,0.5,1,2,5,10]) \\tax1.set_xticklabels([0.1,0.2,0.5,1,2,5,10]) \\tplt.title('$title overlap by AF') \\tplt.subplots_adjust(bottom=0.2,left=0.1,right=0.95) \\tplt.savefig('$img.png') \\tif pdf_plots: plt.savefig('$img.pdf') \\tplt.close() "; } sub plot_indel_distribution { my ($opts,$id) = @_; my @vals = get_values($opts,$id,'IDD'); if ( !@vals ) { return; } # Set xlim to show 99 of indels but ignore outliers my @tmp; for my $id (file_ids($opts)) { my @v = get_values($opts,$id,'IDD'); for my $v (@v) { $tmp[ abs($$v[0]) ] += $$v[1]; } } my $n; for my $t (@tmp) { $n += $t ? $t : 0; } my ($sum,$xlim); for ($xlim=0; $xlim<@tmp; $xlim++) { $sum += $tmp[$xlim] ? $tmp[$xlim] : 0; if ( $sum/$n >= 0.99 ) { last; } } if ( $xlim<20 ) { $xlim=20; } my $fh = $$opts{plt_fh}; my $img = "$$opts{prefix}indels.$id"; open(my $tfh,'>',"$img.dat") or error("$img.dat: $!"); print $tfh "# [1]Indel length\t[2]Count\n"; for my $val (@vals) { print $tfh "$$val[0]\t$$val[1]\n"; } close($tfh); tprint $fh, " dat = [] with open('$img.dat', 'rb') as f: \\treader = csv.reader(f, 'tab') \\tfor row in reader: \\t\\tif row[0][0] != '#': dat.append([float(x) for x in row]) if plot_indel_dist and len(dat)>0: \\tfig = plt.figure(figsize=($$opts{img_width},$$opts{img_height})) \\tax1 = fig.add_subplot(111) \\tax1.bar([row[0]-0.5 for row in dat], [row[1] for row in dat], color='$$opts{id2col}[0]')# , edgecolor='$$opts{id2col}[0]') \\tax1.set_xlabel('InDel Length') \\tax1.set_ylabel('Count') \\tax1.ticklabel_format(style='sci', scilimits=(0,0), axis='y') \\tax1.set_xlim(-$xlim,$xlim) \\tplt.subplots_adjust(bottom=0.17) \\tplt.title('$$opts{title}{$id}') \\tplt.savefig('$img.png') \\tif pdf_plots: plt.savefig('$img.pdf') \\tplt.close() "; } sub plot_substitutions { my ($opts,$id) = @_; my @vals = get_values($opts,$id,'ST'); if ( !@vals ) { return; } my $fh = $$opts{plt_fh}; my $img = "$$opts{prefix}substitutions.$id"; tprint $fh, " dat = [ "; for (my $i=0; $i<@vals; $i++) { my $val=$vals[$i]; tprint $fh, "\t[$i,'$$val[0]',$$val[1]],\n"; } tprint $fh, "] if plot_substitutions: \\tfig = plt.figure(figsize=($$opts{img_width},$$opts{img_height})) \\tcm = mpl.cm.get_cmap('autumn') \\tn = 12 \\tcol = range(n) \\tfor i in range(n): col[i] = cm(1.*i/n) \\tax1 = fig.add_subplot(111) \\tax1.bar([row[0] for row in dat], [row[2] for row in dat], color=col) \\tax1.set_ylabel('Count') \\tax1.ticklabel_format(style='sci', scilimits=(0,0), axis='y') \\tax1.set_xlim(-0.5,n+0.5) \\tplt.xticks([row[0] for row in dat],[row[1] for row in dat],rotation=45) \\tplt.title('$$opts{title}{$id}') \\tplt.savefig('$img.png') \\tif pdf_plots: plt.savefig('$img.pdf') \\tplt.close() "; } sub singletons { my ($opts,$id) = @_; my @si_vals = get_values($opts,$id,'SiS'); my $si_snps = $si_vals[0][1]; my $si_indels = $si_vals[0][4]; my $si_irc = sprintf "%.3f", $si_vals[0][6] ? $si_vals[0][5]/($si_vals[0][5]+$si_vals[0][6]) : 0; my $si_tstv = sprintf "%.2f", $si_vals[0][3] ? $si_vals[0][2]/$si_vals[0][3] : 0; my @all_vals = get_values($opts,$id,'AF'); my $nsnps = 0; my $nindels = 0; for my $val (@all_vals) { $nsnps += $$val[1]; $nindels += $$val[4]; } $si_snps = sprintf "%.1f", $nsnps ? $si_snps*100./$nsnps : 0; $si_indels = sprintf "%.1f", $nindels ? $si_indels*100./$nindels : 0; return { snps=>$si_snps, indels=>$si_indels, tstv=>$si_tstv, irc=>$si_irc }; } sub calc_3n_n3n { my (@vals) = @_; my $n3 = 0; my $nn3 = 0; for my $val (@vals) { if ( !($$val[0]%3) ) { $n3++; } else { $nn3++; } } if ( !$nn3 ) { return '-'; } return sprintf("%.2f", $n3/$nn3); } sub fmt_slide3v { my ($opts, $image, $title) = @_; my $n = 0; for my $id (0..2) { if ( -e "$image.$id.$$opts{fmt}" ) { $n++; } } if ( !$n ) { return ''; } my $h = $$opts{tex}{slide3v}{"height$n"}; my $slide = q[\vbox{]; for my $id (0..2) { if ( !-e "$image.$id.$$opts{fmt}" ) { next; } $slide .= qq[\\centerline{\\includegraphics[$$opts{ext},height=$h]{$image.$id}}]; } $slide .= '}'; return qq[ % $title % \\hslide{$title}{$slide} ]; } sub fmt_slide3h { my ($opts, $image, $title) = @_; my $n = 0; for my $id (0..2) { if ( -e "$image.$id.$$opts{fmt}" ) { $n++; } } if ( !$n ) { return ''; } my $w = $$opts{tex}{slide3h}{"width$n"}; my $slide = ''; for my $id (0..2) { if ( !-e "$image.$id.$$opts{fmt}" ) { next; } $slide .= qq[\\includegraphics[$$opts{ext},width=$w]{$image.$id}]; } return qq[ % $title % \\hslide{$title}{$slide} ]; } sub bignum { my ($num) = @_; if ( !defined $num ) { return ''; } if ( !($num=~/^\d+$/) ) { return $num; } my $len = length($num); my $out; for (my $i=0; $i<$len; $i++) { $out .= substr($num,$i,1); if ( $i+1<$len && !(($len-$i-1)%3) ) { $out .= ','; } } return $out; } sub create_pdf { my ($opts) = @_; chdir($$opts{dir}); my @ids = file_ids($opts); my $width = "25.4cm"; # todo: move all this to $$opts{tex} my $height = "19cm"; my $height1 = "13cm"; my $width1 = "23cm"; my $width2 = @ids==3 ? "10.5cm" : "10.5cm"; my $width3 = @ids==3 ? "8cm" : "15cm"; my $fmt = $$opts{rasterize} ? 'png' : 'pdf'; my $ext = "type=$fmt,ext=.$fmt,read=.$fmt"; my $args = { ext=>$ext, width3=>$width3, n=>scalar @ids }; $$opts{fmt} = $fmt; $$opts{ext} = $ext; # Check that xcolor is available my @has_xcolor = `kpsewhich xcolor.sty`; if ( !@has_xcolor ) { warn("Note: The xcolor.sty package not available, black and white tables only...\n\n"); } my $tex_file = "$$opts{lprefix}summary.tex"; my $pdf_file = "$$opts{prefix}summary.pdf"; open(my $tex,'>',$tex_file) or error("$tex_file: $!"); tprint $tex, qq[ % This file was produced by plot-vcfstats, the command line was: % $$opts{args} % % Edit as necessary and recreate the PDF by running % pdflatex $tex_file % % Slides style and dimensions % \\nonstopmode \\documentclass[17pt]{memoir} \\setstocksize{$height}{$width} \\settrimmedsize{\\stockheight}{\\stockwidth}{*} \\settrims{0pt}{0pt} \\setlrmarginsandblock{1cm}{*}{*} \\setulmarginsandblock{1.5cm}{*}{*} \\setheadfoot{1mm}{1cm} \\setlength{\\parskip}{0pt} \\setheaderspaces{*}{1mm}{*} \\setmarginnotes{1mm}{1mm}{1mm} \\checkandfixthelayout[fixed] \\usepackage{charter} % font \\pagestyle{plain} \\makeevenfoot{plain}{}{}{\\thepage} \\makeoddfoot{plain}{}{}{\\thepage} \\usepackage{graphicx} % For colored tables. If xcolor.sty is not available on your system, % download xcolor.sty.gz LaTeX class style from % http://www.ukern.de/tex/xcolor.html % Unpack and install system-wide or place elsewhere and make available by % setting the TEXINPUTS environment variable (note the colon) % export TEXINPUTS=some/dir: % The list of the recognised path can be obtained by running `kpsepath tex` % \\usepackage{multirow} \\setlength{\\tabcolsep}{0.6em} \\renewcommand{\\arraystretch}{1.2} ]; if ( @has_xcolor ) { tprint $tex, '\usepackage[table]{xcolor}'; } else { tprint $tex, qq[ \\newcommand{\\definecolor}[3]{} \\newcommand{\\columncolor}[1]{} \\newcommand{\\rowcolors}[4]{} \\newcommand{\\arrayrulecolor}[1]{} ]; } tprint $tex, qq[ \\definecolor{hcol1}{rgb}{1,0.6,0} \\definecolor{hcol2}{rgb}{1,0.68,0.2} \\definecolor{row1}{rgb}{1,0.88,0.7} \\definecolor{row2}{rgb}{1,0.92,0.8} % #FFEBCC \\setlength{\\arrayrulewidth}{1.5pt} % Slide headings \\newcommand*{\\head}[1]{{\\Large\\centerline{#1}\\vskip0.5em}} % Slide definition \\newcommand*{\\hslide}[2]{% \\head{#1}% \\begin{vplace}[0.5]\\centerline{#2}\\end{vplace}\\newpage} \\newcommand{\\pdf}[2]{\\IfFileExists{#2.$fmt}{\\includegraphics[#1]{#2}}{}} % The actual slides \\begin{document} ]; # Table with summary numbers my $slide .= q[ \begin{minipage}{\textwidth}\centering \small \rowcolors*{3}{row2}{row1} \arrayrulecolor{black} \begin{tabular}{l | r r r | r r r | r | r} \multicolumn{1}{>{\columncolor{hcol1}}l|}{} & \multicolumn{3}{>{\columncolor{hcol1}}c|}{SNPs} & \multicolumn{3}{>{\columncolor{hcol1}}c|}{indels} & \multicolumn{1}{>{\columncolor{hcol1}}c|}{MNPs} & \multicolumn{1}{>{\columncolor{hcol1}}c}{others} \\\\ % \multicolumn{1}{>{\columncolor{hcol2}}l|}{Callset} & \multicolumn{1}{>{\columncolor{hcol2}}c}{n} & \multicolumn{1}{>{\columncolor{hcol2}}c }{ts/tv} & \multicolumn{1}{>{\columncolor{hcol2}}c|}{\\footnotesize(1st ALT)} & \multicolumn{1}{>{\columncolor{hcol2}}c}{n} & \multicolumn{1}{>{\columncolor{hcol2}}c}{frm$^*$} & \multicolumn{1}{>{\columncolor{hcol2}}c|}{rc$^{**}$} & \multicolumn{1}{>{\columncolor{hcol2}}c|}{} & \multicolumn{1}{>{\columncolor{hcol2}}c}{} \\\\ \hline ]; my %tex_titles; for my $id (@ids) { my $snps = get_value($opts,$id,'number of SNPs:'); my $indels = get_value($opts,$id,'number of indels:'); my $mnps = get_value($opts,$id,'number of MNPs:'); my $others = get_value($opts,$id,'number of others:'); my $tstv = sprintf "%.2f",get_values($opts,$id,'TSTV',0,2); my $tstv1 = sprintf "%.2f",get_values($opts,$id,'TSTV',0,5); my @frsh = get_values($opts,$id,'FS'); my $frsh = @frsh ? $frsh[0][3] : '--'; my @rc = get_values($opts,$id,'ICS'); my $rc = @rc ? sprintf("%.3f",$rc[0][3]) : '--'; my $title = $$opts{title}{$id}; $title =~ s/_/\\_/g; $title =~ s/^\s*\*/\$*\$/; # leading asterisks is eaten by TeX $tex_titles{$id} = $title; $slide .= qq[ $title & ] . bignum($snps) . qq[ & $tstv & $tstv1 & ] . bignum($indels) . qq[ & $frsh & $rc & ] . bignum($mnps) . ' & ' . bignum($others) . qq[ \\\\ \n]; } $slide .= q[% \multicolumn{8}{r}{$^*$ frameshift ratio: out/(out+in);\hspace{1em} $^{**}$ repeat-consistency} \\\\ \end{tabular} \\\\ \vspace{1em} \begin{tabular}{l | r r r r | r r} \multicolumn{1}{>{\columncolor{hcol1}}l|}{} & \multicolumn{4}{>{\columncolor{hcol1}}c|}{singletons {\footnotesize(AC=1)}} & \multicolumn{2}{>{\columncolor{hcol1}}c}{multiallelic} \\\\ % \multicolumn{1}{>{\columncolor{hcol2}}l|}{Callset} & \multicolumn{1}{>{\columncolor{hcol2}}c}{SNPs} & \multicolumn{1}{>{\columncolor{hcol2}}c}{ts/tv} & \multicolumn{1}{>{\columncolor{hcol2}}c}{indels} & \multicolumn{1}{>{\columncolor{hcol2}}c|}{rc} & \multicolumn{1}{>{\columncolor{hcol2}}c}{sites} & \multicolumn{1}{>{\columncolor{hcol2}}c}{SNPs} \\\\ \hline ]; for my $id (@ids) { my $snps = get_value($opts,$id,'number of SNPs:'); my $s = singletons($opts,$id); my $mals = get_value($opts,$id,'number of multiallelic sites:'); my $msnps = get_value($opts,$id,'number of multiallelic SNP sites:'); my $title = $tex_titles{$id}; $slide .= qq[ $title & $$s{snps}\\% & $$s{tstv} & $$s{indels}\\% & $$s{irc} & ] . bignum($mals) . ' &' . bignum($msnps) . qq[ \\\\ \n]; } $slide .= q[ \\end{tabular} \\vspace{2em} \\begin{itemize}[-] \\setlength{\\itemsep}{0pt} ]; for my $id (@ids) { my $fname = $$opts{dat}{ID}{$id}[0][0]; if ( $$opts{title}{$id} =~ / \+ / ) { next; } $fname =~ s/.{80}/$&\\\\\\hskip2em /g; $fname =~ s/_/\\_/g; $slide .= qq[\\item $tex_titles{$id} .. \\texttt{\\footnotesize $fname}\n]; } $slide .= q[\\end{itemize}\\end{minipage}]; my $title = exists($$opts{main_title}) ? $$opts{main_title} : 'Summary Numbers'; tprint $tex, qq[ % Table with summary numbers % \\hslide{$title}{$slide} ]; # Venn bars if ( @ids==3 ) { tprint $tex, qq[% % Venn numbers % \\hslide{Total counts}{% \\includegraphics[$ext,width=$width2]{$$opts{lprefix}venn_bars.snps}% \\includegraphics[$ext,width=$width2]{$$opts{lprefix}venn_bars.indels} } ]; } tprint $tex, fmt_slide3v($opts, "$$opts{lprefix}tstv_by_sample", 'Ts/Tv by sample'); tprint $tex, fmt_slide3v($opts, "$$opts{lprefix}hets_by_sample", 'Hets vs non-ref Homs by sample'); tprint $tex, fmt_slide3v($opts, "$$opts{lprefix}singletons_by_sample", 'Singletons by sample {\normalsize(hets and homs)}'); tprint $tex, fmt_slide3v($opts, "$$opts{lprefix}dp_by_sample", 'Average depth by sample'); tprint $tex, fmt_slide3v($opts, "$$opts{lprefix}snps_by_sample", 'Number of SNPs by sample'); tprint $tex, fmt_slide3v($opts, "$$opts{lprefix}indels_by_sample", 'Number of indels by sample'); if ( scalar get_values($opts,2,'GCsS') ) { tprint $tex, qq[ % Genotype discordance by sample % \\hslide{Genotype discordance by sample}{\\pdf{$ext,width=$width1}{$$opts{lprefix}gts_by_sample}} ]; } if ( scalar get_values($opts,2,'GCsAF') ) { my @vals = get_values($opts,2,'NRDs'); my $nrd = sprintf "%.2f", $vals[0][0]; my $rr = sprintf "%.2f", $vals[0][1]; my $ra = sprintf "%.2f", $vals[0][2]; my $aa = sprintf "%.2f", $vals[0][3]; my $nsamples = get_value($opts,2,'number of samples:'); my $table = qq[% {\\small \\rowcolors*{1}{row2}{row1}\\arrayrulecolor{black} \\begin{tabular}{c | c | c | c } \\multicolumn{1}{>{\\columncolor{hcol1}}c|}{REF/REF} & \\multicolumn{1}{>{\\columncolor{hcol1}}c|}{REF/ALT} & \\multicolumn{1}{>{\\columncolor{hcol1}}c|}{ALT/ALT} & \\multicolumn{1}{>{\\columncolor{hcol1}}c}{NRDs} \\\\ \\hline $rr\\% & $ra\\% & $aa\\% & $nrd\\% \\\\ \\end{tabular}}]; tprint $tex, qq[ % Genotype discordance by AF % \\head{Genotype discordance by AF}\\begin{vplace}[0.7]\\centerline{$table}% \\centerline{\\pdf{$ext,height=$height1}{$$opts{lprefix}gts_by_af}}\\end{vplace} \\newpage % dosage r2 by AF % \\hslide{Allelic R\$^2\$ by AF}{\\pdf{$ext,height=$height1}{$$opts{lprefix}r2_by_af}} ]; } if ( -e "$$opts{lprefix}counts_by_af.snps.$fmt" && -e "$$opts{lprefix}counts_by_af.indels.$fmt" ) { tprint $tex, qq[ % SNP and indel counts by AF % \\hslide{}{\\vbox{\\noindent\\includegraphics[$ext,width=$width1]{$$opts{lprefix}counts_by_af.snps}\\\\% \\noindent\\includegraphics[$ext,width=$width1]{$$opts{lprefix}counts_by_af.indels}}} ]; } if ( -e "$$opts{lprefix}overlap_by_af.snps.$fmt" && -e "$$opts{lprefix}overlap_by_af.indels.$fmt" ) { tprint $tex, qq[ % SNP and indel overlap by AF % \\hslide{}{\\vbox{\\noindent\\includegraphics[$ext,width=$width1]{$$opts{lprefix}overlap_by_af.snps}\\\\% \\noindent\\includegraphics[$ext,width=$width1]{$$opts{lprefix}overlap_by_af.indels}}} ]; } tprint $tex, fmt_slide3h($opts, "$$opts{lprefix}tstv_by_af", 'Ts/Tv by AF'); tprint $tex, fmt_slide3h($opts, "$$opts{lprefix}tstv_by_qual", 'Ts/Tv stratified by QUAL'); tprint $tex, fmt_slide3h($opts, "$$opts{lprefix}indels", 'Indel distribution'); tprint $tex, fmt_slide3h($opts, "$$opts{lprefix}depth", 'Depth distribution'); tprint $tex, fmt_slide3h($opts, "$$opts{lprefix}hwe", 'Number of HETs by AF'); tprint $tex, fmt_slide3h($opts, "$$opts{lprefix}substitutions", 'Substitution types'); tprint $tex, fmt_slide3h($opts, "$$opts{lprefix}irc_by_af", 'Indel Repeat Consistency by AF'); tprint $tex, fmt_slide3h($opts, "$$opts{lprefix}irc_by_rlen", 'Indel Consistency by Repeat Type'); tprint $tex, "\n\n\\end{document}\n"; close($tex); $tex_file =~ s{^.+/}{}; my $cmd = "pdflatex $tex_file >$$opts{logfile} 2>&1"; print STDERR "Creating PDF: $cmd\n" unless !$$opts{verbose}; system($cmd); if ( $? ) { error("The command exited with non-zero status, please consult the output of pdflatex: $$opts{dir}$$opts{logfile}\n\n"); } print STDERR "Finished: $pdf_file\n" unless !$$opts{verbose}; } sub merge_vcfstats { my ($opts) = @_; my $fh = *STDOUT; if ( !$$opts{merge} ) { open($fh,'>',"$$opts{prefix}merge.chk") or error("$$opts{prefix}merge.chk: $!\n"); } print $fh "# This file was produced by plot-vcfstats, the command line was:\n# $$opts{args}\n#\n"; for my $sec (@{$$opts{sections}}) { my $sid = $$sec{id}; if ( !exists($$opts{dat}{$sid}) ) { next; } print $fh "# $$sec{header}\n$$sec{exp}\n"; for my $id (sort keys %{$$opts{dat}{$sid}}) { for my $rec (@{$$opts{dat}{$sid}{$id}}) { print $fh "$sid\t$id\t", join("\t",@$rec), "\n"; } } if ( $sid eq 'ID' ) { print $fh "# $$opts{id2sec}{SN}{header}\n$$opts{id2sec}{SN}{exp}\n"; # output summary numbers here for my $id (keys %{$$opts{dat}}) { if ( exists($$opts{exp}{$id}) ) { next; } for my $key (keys %{$$opts{dat}{$id}}) { print $fh "SN\t$id\t$key\t$$opts{dat}{$id}{$key}\n"; } } } } } bcftools-1.2/plugins/000077500000000000000000000000001246371514100146545ustar00rootroot00000000000000bcftools-1.2/plugins/counts.c000066400000000000000000000050031246371514100163310ustar00rootroot00000000000000/* plugins/counts.c -- counts SNPs, Indels, and total number of sites. Copyright (C) 2013, 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include int nsamples, nsnps, nindels, nmnps, nothers, nsites; /* This short description is used to generate the output of `bcftools plugin -l`. */ const char *about(void) { return "A minimal plugin which counts number of samples, SNPs,\n" "INDELs, MNPs and total number of sites.\n"; } /* Called once at startup, allows to initialize local variables. Return 1 to suppress VCF/BCF header from printing, 0 otherwise. */ int init(int argc, char **argv, bcf_hdr_t *in, bcf_hdr_t *out) { nsamples = bcf_hdr_nsamples(in); nsnps = nindels = nmnps = nothers = nsites = 0; return 1; } /* Called for each VCF record. Return rec to output the line or NULL to suppress output. */ bcf1_t *process(bcf1_t *rec) { int type = bcf_get_variant_types(rec); if ( type & VCF_SNP ) nsnps++; if ( type & VCF_INDEL ) nindels++; if ( type & VCF_MNP ) nmnps++; if ( type & VCF_OTHER ) nothers++; nsites++; return NULL; } /* Clean up. */ void destroy(void) { printf("Number of samples: %d\n", nsamples); printf("Number of SNPs: %d\n", nsnps); printf("Number of INDELs: %d\n", nindels); printf("Number of MNPs: %d\n", nmnps); printf("Number of others: %d\n", nothers); printf("Number of sites: %d\n", nsites); } bcftools-1.2/plugins/dosage.c000066400000000000000000000200721246371514100162630ustar00rootroot00000000000000/* plugins/dosage.c -- prints genotype dosage. Copyright (C) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include /* This short description is used to generate the output of `bcftools plugin -l`. */ const char *about(void) { return "Prints genotype dosage determined from tags requested by the user.\n"; } const char *usage(void) { return "\n" "About: Print genotype dosage\n" "Usage: bcftools +dosage [General Options] -- [Plugin Options]\n" "Options:\n" " run \"bcftools plugin\" for a list of common options\n" "\n" "Plugin options:\n" " -t, --tags VCF tags to determine the dosage from [PL,GL,GT]\n" "\n" "Example:\n" " bcftools +dosage in.vcf -- -t GT\n" "\n"; } bcf_hdr_t *in_hdr = NULL; int pl_type = 0, gl_type = 0; uint8_t *buf = NULL; int nbuf = 0; // NB: number of elements, not bytes char **tags = NULL; int ntags = 0; typedef int (*dosage_f) (bcf1_t *); dosage_f *handlers = NULL; int nhandlers = 0; int calc_dosage_PL(bcf1_t *rec) { int i, j, nret = bcf_get_format_values(in_hdr,rec,"PL",(void**)&buf,&nbuf,pl_type); if ( nret<0 ) return -1; nret /= rec->n_sample; #define BRANCH(type_t,is_missing,is_vector_end) \ { \ type_t *ptr = (type_t*) buf; \ for (i=0; in_sample; i++) \ { \ float vals[3] = {0,0,0}; \ for (j=0; jn_sample; #define BRANCH(type_t,is_missing,is_vector_end) \ { \ type_t *ptr = (type_t*) buf; \ for (i=0; in_sample; i++) \ { \ float vals[3] = {0,0,0}; \ for (j=0; jn_sample; int32_t *ptr = (int32_t*) buf; for (i=0; in_sample; i++) { float dsg = 0; for (j=0; j0 ? dsg : -1); ptr += nret; } return 0; } char **split_list(char *str, int *nitems) { int n = 0, done = 0; char *ss = strdup(str), **out = NULL; while ( !done && *ss ) { char *se = ss; while ( *se && *se!=',' ) se++; if ( !*se ) done = 1; *se = 0; n++; out = (char**) realloc(out,sizeof(char*)*n); out[n-1] = ss; ss = se+1; } *nitems = n; return out; } int init(int argc, char **argv, bcf_hdr_t *in, bcf_hdr_t *out) { int i, id, c; char *tags_str = "PL,GL,GT"; static struct option loptions[] = { {"tags",1,0,'t'}, {0,0,0,0} }; while ((c = getopt_long(argc, argv, "t:?h",loptions,NULL)) >= 0) { switch (c) { case 't': tags_str = optarg; break; case 'h': case '?': default: fprintf(stderr,"%s", usage()); exit(1); break; } } tags = split_list(tags_str, &ntags); in_hdr = in; for (i=0; isamples[i]); printf("\n"); return 1; } bcf1_t *process(bcf1_t *rec) { int i, ret; printf("%s\t%d\t%s\t%s", bcf_seqname(in_hdr,rec),rec->pos+1,rec->d.allele[0],rec->n_allele>1 ? rec->d.allele[1] : "."); if ( rec->n_allele==1 ) { for (i=0; in_sample; i++) printf("\t0.0"); } else { for (i=0; in_sample; i++) printf("\t-1.0"); } } printf("\n"); return NULL; } void destroy(void) { free(handlers); free(buf); } bcftools-1.2/plugins/fill-AN-AC.c000066400000000000000000000042421246371514100165250ustar00rootroot00000000000000/* plugins/fill-AN-AC.c -- fills AN and AC INFO fields. Copyright (C) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include bcf_hdr_t *in_hdr, *out_hdr; int *arr = NULL, marr = 0; const char *about(void) { return "Fill INFO fields AN and AC.\n"; } int init(int argc, char **argv, bcf_hdr_t *in, bcf_hdr_t *out) { in_hdr = in; out_hdr = out; bcf_hdr_append(out_hdr, "##INFO="); bcf_hdr_append(out_hdr, "##INFO="); return 0; } bcf1_t *process(bcf1_t *rec) { hts_expand(int,rec->n_allele,marr,arr); int ret = bcf_calc_ac(in_hdr,rec,arr,BCF_UN_FMT); if ( ret>0 ) { int i, an = 0; for (i=0; in_allele; i++) an += arr[i]; bcf_update_info_int32(out_hdr, rec, "AN", &an, 1); bcf_update_info_int32(out_hdr, rec, "AC", arr+1, rec->n_allele-1); } return rec; } void destroy(void) { free(arr); } bcftools-1.2/plugins/fixploidy.c000066400000000000000000000166441246371514100170420ustar00rootroot00000000000000/* Copyright (C) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include "bcftools.h" #include "ploidy.h" static bcf_hdr_t *in_hdr = NULL, *out_hdr = NULL; static int *sample2sex = NULL; static int n_sample = 0, nsex = 0, *sex2ploidy = NULL; static int32_t ngt_arr = 0, *gt_arr = NULL, *gt_arr2 = NULL, ngt_arr2 = 0; static ploidy_t *ploidy = NULL; const char *about(void) { return "Fix ploidy.\n"; } const char *usage(void) { return "\n" "About: Fix ploidy\n" "Usage: bcftools +fixploidy [General Options] -- [Plugin Options]\n" "Options:\n" " run \"bcftools plugin\" for a list of common options\n" "\n" "Plugin options:\n" " -p, --ploidy space/tab-delimited list of CHROM,FROM,TO,SEX,PLOIDY\n" " -s, --sex list of samples, \"NAME SEX\"\n" " -t, --tags VCF tags to fix [GT]\n" "\n" "Example:\n" " # Default ploidy, if -p not given. Unlisted regions have ploidy 2\n" " X 1 60000 M 1\n" " X 2699521 154931043 M 1\n" " Y 1 59373566 M 1\n" " Y 1 59373566 F 0\n" " MT 1 16569 M 1\n" " MT 1 16569 F 1\n" " \n" " # Example of -s file, sex of unlisted samples is \"F\"\n" " sampleName1 M\n" " \n" " bcftools +fixploidy in.vcf -- -s samples.txt\n" "\n"; } void set_samples(char *fname, bcf_hdr_t *hdr, ploidy_t *ploidy, int *sample2sex) { kstring_t tmp = {0,0,0}; htsFile *fp = hts_open(fname, "r"); if ( !fp ) error("Could not read: %s\n", fname); while ( hts_getline(fp, KS_SEP_LINE, &tmp) > 0 ) { char *ss = tmp.s; while ( *ss && isspace(*ss) ) ss++; if ( !*ss ) error("Could not parse: %s\n", tmp.s); if ( *ss=='#' ) continue; char *se = ss; while ( *se && !isspace(*se) ) se++; char x = *se; *se = 0; int ismpl = bcf_hdr_id2int(hdr, BCF_DT_SAMPLE, ss); if ( ismpl < 0 ) { fprintf(stderr,"Warning: No such sample in the VCF: %s\n",ss); continue; } *se = x; ss = se+1; while ( *ss && isspace(*ss) ) ss++; if ( !*ss ) error("Could not parse: %s\n", tmp.s); se = ss; while ( *se && !isspace(*se) ) se++; if ( se==ss ) error("Could not parse: %s\n", tmp.s); sample2sex[ismpl] = ploidy_add_sex(ploidy, ss); } if ( hts_close(fp) ) error("Close failed: %s\n", fname); free(tmp.s); } int init(int argc, char **argv, bcf_hdr_t *in, bcf_hdr_t *out) { int c; char *tags_str = "GT"; char *ploidy_fname = NULL, *sex_fname = NULL; static struct option loptions[] = { {"ploidy",1,0,'p'}, {"sex",1,0,'s'}, {"tags",1,0,'t'}, {0,0,0,0} }; while ((c = getopt_long(argc, argv, "?ht:s:p:",loptions,NULL)) >= 0) { switch (c) { case 'p': ploidy_fname = optarg; break; case 's': sex_fname = optarg; break; case 't': tags_str = optarg; break; case 'h': case '?': default: error("%s", usage()); break; } } if ( strcasecmp("GT",tags_str) ) error("Only -t GT is currently supported, sorry\n"); n_sample = bcf_hdr_nsamples(in); sample2sex = (int*) calloc(n_sample,sizeof(int)); in_hdr = in; out_hdr = out; if ( ploidy_fname ) ploidy = ploidy_init(ploidy_fname, 2); else { ploidy = ploidy_init_string( "X 1 60000 M 1\n" "X 2699521 154931043 M 1\n" "Y 1 59373566 M 1\n" "Y 1 59373566 F 0\n" "MT 1 16569 M 1\n" "MT 1 16569 F 1\n", 2); } if ( !ploidy ) return -1; // add default sex in case it was not included int i, dflt_sex_id = ploidy_add_sex(ploidy, "F"); for (i=0; ipos, sex2ploidy,NULL,&max_ploidy); int ngts = bcf_get_genotypes(in_hdr, rec, >_arr, &ngt_arr); if ( ngts % n_sample ) error("Error at %s:%d: wrong number of GT fields\n",bcf_seqname(in_hdr,rec),rec->pos+1); ngts /= n_sample; if ( ngts < max_ploidy ) { hts_expand(int32_t,max_ploidy*n_sample,ngt_arr2,gt_arr2); for (i=0; ipos+1); } else if ( ngts!=1 || max_ploidy!=1 ) { for (i=0; ipos+1); } return rec; } void destroy(void) { free(gt_arr); free(gt_arr2); free(sample2sex); free(sex2ploidy); ploidy_destroy(ploidy); } bcftools-1.2/plugins/fixploidy.mk000066400000000000000000000002751246371514100172200ustar00rootroot00000000000000plugins/fixploidy.so: plugins/fixploidy.c version.h version.c ploidy.h ploidy.c $(HTSDIR)/libhts.so $(CC) $(CFLAGS) $(INCLUDES) -fPIC -shared -o $@ ploidy.c version.c $< -L$(HTSDIR) -lhts bcftools-1.2/plugins/frameshifts.c000066400000000000000000000113711246371514100173360ustar00rootroot00000000000000/* plugins/frameshifts.c -- annotates frameshift indels. Copyright (C) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include bcf_hdr_t *in_hdr, *out_hdr; bcf_sr_regions_t *exons; int32_t *frm = NULL, nfrm = 0; const char *about(void) { return "Annotate frameshift indels.\n"; } const char *usage(void) { return "\n" "About: Annotate frameshift indels\n" "Usage: bcftools +frameshifts [General Options] -- [Plugin Options]\n" "Options:\n" " run \"bcftools plugin\" for a list of common options\n" "\n" "Plugin options:\n" " -e, --exons list of exons, see \"--targets-file\" man page entry for details\n" "\n" "Example:\n" " bcftools +frameshifts in.vcf -- -e exons.bed.gz\n" "\n"; } int init(int argc, char **argv, bcf_hdr_t *in, bcf_hdr_t *out) { int c; char *fname = NULL; static struct option loptions[] = { {"exons",1,0,'e'}, {0,0,0,0} }; while ((c = getopt_long(argc, argv, "e:?h",loptions,NULL)) >= 0) { switch (c) { case 'e': fname = optarg; break; case 'h': case '?': default: fprintf(stderr,"%s", usage()); exit(1); break; } } if ( !fname ) { fprintf(stderr,"Missing the -e option.\n"); return -1; } in_hdr = in; out_hdr = out; int ret = bcf_hdr_append(out_hdr,"##INFO="); if ( ret!=0 ) { fprintf(stderr,"Error updating the header\n"); return -1; } exons = bcf_sr_regions_init(fname,1,0,1,2); if ( !exons ) { fprintf(stderr,"Error occurred while reading (was the file compressed with bgzip?): %s\n", fname); return -1; } return 0; } bcf1_t *process(bcf1_t *rec) { if ( rec->n_allele<2 ) return rec; // not a variant int type = bcf_get_variant_types(rec); if ( !(type&VCF_INDEL) ) return rec; // not an indel int i, len = 0; for (i=1; in_allele; i++) if ( len > rec->d.var[i].n ) len = rec->d.var[i].n; int pos_to = len!=0 ? rec->pos : rec->pos - len; // len is negative if ( bcf_sr_regions_overlap(exons, bcf_seqname(in_hdr,rec),rec->pos,pos_to) ) return rec; // no overlap hts_expand(int32_t,rec->n_allele-1,nfrm,frm); for (i=1; in_allele; i++) { if ( rec->d.var[i].type!=VCF_INDEL ) { frm[i-1] = -1; continue; } int len = rec->d.var[i].n, tlen = 0; if ( len>0 ) { // insertion if ( exons->start <= rec->pos && exons->end > rec->pos ) tlen = abs(len); } else if ( exons->start <= rec->pos + abs(len) ) { // deletion tlen = abs(len); if ( rec->pos < exons->start ) // trim the beginning tlen -= exons->start - rec->pos + 1; if ( exons->end < rec->pos + abs(len) ) // trim the end tlen -= rec->pos + abs(len) - exons->end; } if ( tlen ) // there are some deleted/inserted bases in the exon { if ( tlen%3 ) frm[i-1] = 1; // out-of-frame else frm[i-1] = 0; // in-frame } else frm[i-1] = -1; // not applicable (is outside) } if ( bcf_update_info_int32(out_hdr,rec,"OOF",frm,rec->n_allele-1)<0 ) { fprintf(stderr, "Could not annotate OOF :-/\n"); exit(1); } return rec; } void destroy(void) { bcf_sr_regions_destroy(exons); } bcftools-1.2/plugins/missing2ref.c000066400000000000000000000101361246371514100172510ustar00rootroot00000000000000/* plugins/missing2ref.c -- sets missing genotypes to reference allele. Copyright (C) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include bcf_hdr_t *in_hdr, *out_hdr; int32_t *gts = NULL, mgts = 0; int *arr = NULL, marr = 0; uint64_t nchanged = 0; int new_gt = bcf_gt_unphased(0); int use_major = 0; const char *about(void) { return "Set missing genotypes (\"./.\") to ref or major allele (\"0/0\" or \"0|0\").\n"; } const char *usage(void) { return "\n" "About: Set missing genotypes\n" "Usage: bcftools +missing2ref [General Options] -- [Plugin Options]\n" "Options:\n" " run \"bcftools plugin\" for a list of common options\n" "\n" "Plugin options:\n" " -p, --phased Set to \"0|0\" \n" " -m, --major Set to major allele \n" "\n" "Example:\n" " bcftools +missing2ref in.vcf -- -p\n" " bcftools +missing2ref in.vcf -- -p -m\n" "\n"; } int init(int argc, char **argv, bcf_hdr_t *in, bcf_hdr_t *out) { int c; static struct option loptions[] = { {"phased",0,0,'p'}, {"major",0,0,'m'}, {0,0,0,0} }; while ((c = getopt_long(argc, argv, "mp?h",loptions,NULL)) >= 0) { switch (c) { case 'p': new_gt = bcf_gt_phased(0); break; case 'm': use_major = 1; break; case 'h': case '?': default: fprintf(stderr,"%s", usage()); exit(1); break; } } in_hdr = in; out_hdr = out; return 0; } bcf1_t *process(bcf1_t *rec) { int ngts = bcf_get_genotypes(in_hdr, rec, >s, &mgts); int i, changed = 0; // Calculating allele frequency for each allele and determining major allele // only do this if use_major is true int majorAllele = -1; int maxAC = -1; int an = 0; if(use_major == 1){ hts_expand(int,rec->n_allele,marr,arr); int ret = bcf_calc_ac(in_hdr,rec,arr,BCF_UN_FMT); if(ret > 0){ for(i=0; i < rec->n_allele; ++i){ an += arr[i]; if(*(arr+i) > maxAC){ maxAC = *(arr+i); majorAllele = i; } } } else{ fprintf(stderr,"Warning: Could not calculate allele count at position %d\n", rec->pos); exit(1); } // replacing new_gt by major allele if(bcf_gt_is_phased(new_gt)) new_gt = bcf_gt_phased(majorAllele); else new_gt = bcf_gt_unphased(majorAllele); } // replace gts for (i=0; i Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include "bcftools.h" #define GP_TO_GL 1 static int mode = 0, drop_source_tag = 0; static bcf_hdr_t *in_hdr, *out_hdr; static float *farr = NULL; static int mfarr = 0; const char *about(void) { return "Convert between similar tags, such as GL and GP.\n"; } const char *usage(void) { return "\n" "About: Convert between similar tags, such as GL and GP.\n" "Usage: bcftools +tag2tag [General Options] -- [Plugin Options]\n" "Options:\n" " run \"bcftools plugin\" for a list of common options\n" "\n" "Plugin options:\n" //todo " --gl-to-gp convert FORMAT/GL to FORMAT/GP\n" " --gp-to-gl convert FORMAT/GP to FORMAT/GL\n" " -r, --replace drop the source tag\n" "\n" "Example:\n" " bcftools +tag2tag in.vcf -- -r --gp-to-gl\n" "\n"; } static void init_header(bcf_hdr_t *hdr, const char *ori, int ori_type, const char *new_hdr_line) { if ( ori ) bcf_hdr_remove(hdr,ori_type,ori); bcf_hdr_append(hdr, new_hdr_line); } int init(int argc, char **argv, bcf_hdr_t *in, bcf_hdr_t *out) { static struct option loptions[] = { {"replace",0,0,'r'}, {"gp-to-gl",0,0,1}, {0,0,0,0} }; char c; while ((c = getopt_long(argc, argv, "?hr",loptions,NULL)) >= 0) { switch (c) { case 1 : mode = GP_TO_GL; break; case 'r': drop_source_tag = 1; break; case 'h': case '?': default: error("%s", usage()); break; } } if ( !mode ) mode = GP_TO_GL; in_hdr = in; out_hdr = out; if ( mode==GP_TO_GL ) init_header(out_hdr,drop_source_tag?"GP":NULL,BCF_HL_FMT,"##FORMAT="); return 0; } bcf1_t *process(bcf1_t *rec) { int i, n; if ( mode==GP_TO_GL ) { n = bcf_get_format_float(in_hdr,rec,"GP",&farr,&mfarr); for (i=0; i Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include "bcftools.h" #include "ploidy.h" typedef struct { int nsites, nsex, *sex2ploidy, dflt_ploidy, max_ploidy, guess; int ncounts, *counts, nsample, verbose; float *sex2prob, min_hets; int32_t *gts, ngts; bcf_srs_t *sr; bcf_hdr_t *hdr; ploidy_t *ploidy; } args_t; const char *about(void) { return "Determine sample sex by checking genotypes in haploid regions.\n"; } const char *usage(void) { return "\n" "About: Determine sample sex by either checking the presence of haploid/diploid\n" " genotypes (requires correct ploidy in the VCF) or by counting homs/hets\n" " in haploid regions.\n" "Usage: bcftools +vcf2sex -- [Plugin Options]\n" "Plugin options:\n" " -g, --guess do not trust genotypes ploidy, count hom/hets\n" " -m, --min-hets minimum fraction of hets in diploid regions [0.05]\n" " -n, --nsites number of sites to check per region (ignored with -g) [10]\n" " -p, --ploidy space/tab-delimited list of CHROM,FROM,TO,SEX,PLOIDY\n" "\n" "Example:\n" " # Default ploidy, if -p not given. Unlisted regions have ploidy 2\n" " X 1 60000 M 1\n" " X 2699521 154931043 M 1\n" " Y 1 59373566 M 1\n" " Y 1 59373566 F 0\n" " \n" " bcftools +vcf2sex in.vcf.gz\n" " bcftools +vcf2sex in.vcf.gz -- -n 10\n" "\n"; } int process_region_precise(args_t *args, char *seq, regitr_t *itr) { int k = 1; uint32_t start = itr->reg[itr->i].start, end = itr->reg[itr->i].end; while ( itr->i+kn && start==itr->reg[itr->i+k].start && end==itr->reg[itr->i+k].end ) k++; int ret = ploidy_query(args->ploidy, seq, start, args->sex2ploidy, NULL, NULL); assert(ret); memset(args->counts,0,args->ncounts*sizeof(int)); // Select 'nsites' sites spaced so that they evenly cover the whole region // to get a representative sample. We index-jump as we should be checking // a few sites only. int i, rid = -1, pos, prev_pos = -1, ismpl; for (i=0; insites; i++) { rid = -1; pos = ((i+1.0)/(args->nsites+1))*(end - start) + start; if ( i>0 && pos <= prev_pos ) continue; // the vcf is too sparse if ( bcf_sr_seek(args->sr,seq,pos)!=0 ) return k; // sequence not present if ( !bcf_sr_next_line(args->sr) ) return k; // no sites found bcf1_t *rec = bcf_sr_get_line(args->sr,0); if ( rid==-1 ) rid = rec->rid; if ( rid!=rec->rid || rec->pos > end ) break; prev_pos = rec->pos; int ngts = bcf_get_genotypes(args->hdr,rec,&args->gts,&args->ngts); ngts /= args->nsample; for (ismpl=0; ismplnsample; ismpl++) { int32_t *gts = args->gts + ngts*ismpl; int igt, ploidy = 0; for (igt=0; igtcounts[ismpl*(args->max_ploidy+1) + ploidy]++; if ( args->verbose ) fprintf(stderr,"%s:%d\t%s\tploidy=%d\n", seq,rec->pos+1,args->hdr->samples[ismpl],ploidy); } } for (ismpl=0; ismplnsample; ismpl++) { float sum = 0, *probs = args->sex2prob + ismpl*args->nsex; int *counts = args->counts + ismpl*(args->max_ploidy+1); for (i=0; imax_ploidy+1; i++) sum += counts[i]; if ( !sum ) continue; for (i=0; insex; i++) { int ploidy = args->sex2ploidy[i]; probs[i] *= counts[ploidy]/sum; } } return k; } int process_region_guess(args_t *args, char *seq, regitr_t *itr) { int ismpl, k = 1; uint32_t start = itr->reg[itr->i].start, end = itr->reg[itr->i].end; while ( itr->i+kn && start==itr->reg[itr->i+k].start && end==itr->reg[itr->i+k].end ) k++; int ret = ploidy_query(args->ploidy, seq, start, args->sex2ploidy, NULL, NULL); assert(ret); memset(args->counts,0,args->ncounts*sizeof(int)); if ( bcf_sr_seek(args->sr,seq,start)!=0 ) return k; // sequence not present int rid = bcf_hdr_name2id(args->hdr,seq); while ( bcf_sr_next_line(args->sr) ) { bcf1_t *rec = bcf_sr_get_line(args->sr,0); if ( rec->rid!=rid || rec->pos > end ) break; bcf_fmt_t *fmt = bcf_get_fmt(args->hdr, rec, "GT"); for (ismpl=0; ismplnsample; ismpl++) { int gt = bcf_gt_type(fmt, ismpl, NULL,NULL); if ( gt==GT_UNKN ) args->counts[ismpl*3+0]++; // missing else if ( gt==GT_HET_RA || gt==GT_HET_AA ) args->counts[ismpl*3+1]++; // het else args->counts[ismpl*3+2]++; // hom } } for (ismpl=0; ismplnsample; ismpl++) { float sum = 0, *probs = args->sex2prob + ismpl*args->nsex; int i, *counts = args->counts + ismpl*(args->max_ploidy+1); float fhet = (counts[1]+counts[2]) ? (float)counts[1]/(counts[1]+counts[2]) : 0; for (i=0; imax_ploidy+1; i++) sum += counts[i]; for (i=0; insex; i++) { // a very simple heuristics to determine sex by counting hets/homs/missing sites, // in human nhet/nhom ~ 0.2 int ploidy = args->sex2ploidy[i]; float prob = 1; if ( ploidy==0 ) prob = sum ? counts[0] / sum : 1; // fraction of missing sites else if ( ploidy==1 ) { if ( counts[1]+counts[2] ) prob = fhet > args->min_hets ? 0.1 : 0.9; prob *= sum ? 1 - counts[0] / sum : 1./args->nsex; } else { if ( counts[1]+counts[2] ) prob = fhet > args->min_hets ? 0.9 : 0.1; prob *= sum ? 1 - counts[0] / sum : 1./args->nsex; } probs[i] *= prob; } if ( args->verbose ) printf("DBG\t%s:%d-%d\t%s\t%f\t%d\t%d\t%d\n", seq,start+1,end+1,args->hdr->samples[ismpl], fhet,counts[0],counts[1],counts[2]); } return k; } int run(int argc, char **argv) { args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->nsites = 10; args->min_hets = 0.05; static struct option loptions[] = { {"verbose",1,0,'v'}, {"ploidy",1,0,'p'}, {"nsites",1,0,'n'}, {"guess",0,0,'g'}, {"min-hets",1,0,'m'}, {0,0,0,0} }; char c, *tmp, *ploidy_fname = NULL; while ((c = getopt_long(argc, argv, "p:n:gm:v",loptions,NULL)) >= 0) { switch (c) { case 'v': args->verbose = 1; break; case 'g': args->guess = 1; break; case 'm': args->min_hets = strtod(optarg,&tmp); if ( *tmp ) error("Unexpected argument to --min-hets: %s\n", optarg); break; case 'p': ploidy_fname = optarg; break; case 'n': args->nsites = strtol(optarg,&tmp,10); if (*tmp) error("Unexpected argument to --nsites: %s\n", optarg); break; case 'h': case '?': default: error("%s", usage()); break; } } args->sr = bcf_sr_init(); args->sr->require_index = 1; if ( !argv[0] || !bcf_sr_add_reader(args->sr,argv[0]) ) error("%s", usage()); args->hdr = args->sr->readers[0].header; args->nsample = bcf_hdr_nsamples(args->hdr); args->dflt_ploidy = 2; if ( ploidy_fname ) { args->ploidy = ploidy_init(ploidy_fname, args->dflt_ploidy); if ( !args->ploidy ) error("Could not read %s\n", ploidy_fname); } else { args->ploidy = ploidy_init_string( "X 1 60000 M 1\n" "X 2699521 154931043 M 1\n" "Y 1 59373566 M 1\n" "Y 1 59373566 F 0\n", args->dflt_ploidy); } args->nsex = ploidy_nsex(args->ploidy); args->sex2ploidy = (int*) malloc(sizeof(int)*args->nsex); args->max_ploidy = ploidy_max(args->ploidy); if ( args->guess && args->max_ploidy > 2 ) error("Sorry, ploidy %d not supported with -g\n", args->max_ploidy); args->ncounts = args->nsample * ((args->max_ploidy>2 ? args->max_ploidy : 2)+1); args->counts = (int*) malloc(sizeof(int)*args->ncounts); args->sex2prob = (float*) calloc(args->nsample*args->nsex,sizeof(float)); int i, nseq; for (i=0; insample*args->nsex; i++) args->sex2prob[i] = 1; if ( args->verbose && args->guess ) printf("# [1]DBG\t[2]Region\t[3]Sample\t[4]HET fraction\t[5]nHet\t[6]nHom\t[7]nMissing\n"); regidx_t *idx = ploidy_regions(args->ploidy); char **seqs = regidx_seq_names(idx, &nseq); for (i=0; iguess ) itr.i += process_region_guess(args, seqs[i], &itr); else itr.i += process_region_precise(args, seqs[i], &itr); } for (i=0; insample; i++) { int j, jmax = 0; float max = 0, sum = 0; for (j=0; jnsex; j++) { sum += args->sex2prob[i*args->nsex+j]; if ( max < args->sex2prob[i*args->nsex+j] ) { jmax = j; max = args->sex2prob[i*args->nsex+j]; } } if ( args->verbose ) printf("%s\t%s\t%f\n", args->hdr->samples[i],ploidy_id2sex(args->ploidy,jmax),args->sex2prob[i*args->nsex+jmax]/sum); else printf("%s\t%s\n", args->hdr->samples[i],ploidy_id2sex(args->ploidy,jmax)); } bcf_sr_destroy(args->sr); ploidy_destroy(args->ploidy); free(args->sex2ploidy); free(args->counts); free(args->gts); free(args->sex2prob); free(args); return 0; } bcftools-1.2/plugins/vcf2sex.mk000066400000000000000000000002711246371514100165650ustar00rootroot00000000000000plugins/vcf2sex.so: plugins/vcf2sex.c version.h version.c ploidy.h ploidy.c $(HTSDIR)/libhts.so $(CC) $(CFLAGS) $(INCLUDES) -fPIC -shared -o $@ ploidy.c version.c $< -L$(HTSDIR) -lhts bcftools-1.2/polysomy.c000066400000000000000000000624371246371514100152460ustar00rootroot00000000000000/* The MIT License Copyright (c) 2013-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include #include "bcftools.h" typedef struct { int nvals; // number of data points to fit against (excluding RR,AA peaks) double *xvals; // xvalues, pointer to dist_t.xvals double *yvals; // yvalues, pointer to dist_t.yvals int ngauss; // number of gaussian functions } data_t; typedef struct { data_t dat; int nvals; // all values, including RR,AA peaks double *xvals; // pointer to args_t.xvals double *yvals; int copy_number; // heuristics to skip futile CN1 fits when no het peak is detected int irr, iaa; // chop off RR and AA peaks char *chr; } dist_t; typedef struct { int ndist, nbins; double *xvals; dist_t *dist; char **argv, *output_dir; double fit_th, peak_symmetry, cn_penalty; int argc, plot, verbose, regions_is_file, targets_is_file; char *dat_fname, *fname, *regions_list, *targets_list, *sample; FILE *dat_fp; } args_t; FILE *open_file(char **fname, const char *mode, const char *fmt, ...); static void init_dist(dist_t *dist, int verbose) { // isolate RR and AA peaks and rescale so that they are comparable to hets int i, irr, iaa, n = dist->nvals; // smooth the distribution double *tmp = (double*) malloc(sizeof(double)*n); int win = 0.02*n < 1 ? 1 : 0.02*n; double avg = 0; for (i=0; iyvals[i]; for (i=0; iyvals[i] + dist->yvals[i+win]; } for (; i=n/2; i--) if ( tmp[i] < tmp[iaa] ) iaa = i; irr += win*0.5; iaa += win*0.5; if ( irr>=iaa ) error("FIXME: oops, dist normalization failed for %s: %d vs %d\n", dist->chr,irr,iaa); // we may need to be smarter free(tmp); // find the maximum and scale the peaks (first draft: no attempt to join the segments smootly) double max_rr = 0, max_aa = 0, max_ra = 0, srr = 0, saa = 0, sra = 0; for (i=0; iyvals[i]; if ( max_rr < dist->yvals[i] ) max_rr = dist->yvals[i]; } for (i=irr; i<=iaa; i++) { sra += dist->yvals[i]; if ( max_ra < dist->yvals[i] ) max_ra = dist->yvals[i]; } for (i=iaa+1; iyvals[i]; if ( max_aa < dist->yvals[i] ) max_aa = dist->yvals[i]; } // Does the het peak exist at all? Usually the numbers are as follows: // 1: cn=0 ra/rr=0.205730 aa/ra=0.674922 nra=7066 // 20: cn=0 ra/rr=0.258019 aa/ra=0.548929 nra=2381 // X: cn=0 ra/rr=0.005976 aa/ra=44.116667 nra=60 // Y: cn=0 ra/rr=0.008316 aa/ra=7.250000 nra=12 // MT: cn=0 ra/rr=0.013699 aa/ra=0.666667 nra=3 if ( !sra || (sra/srr<0.1 && saa/sra>1.0) ) { max_ra = max_aa; dist->copy_number = 1; } else if ( sra/srr<0.1 || saa/sra>1.0 ) { max_ra = max_aa; dist->copy_number = -1; // unknown copy number } if ( max_rr ) for (i=0; iyvals[i] /= max_rr; if ( max_ra ) for (i=irr; i<=iaa; i++) dist->yvals[i] /= max_ra; if ( max_aa ) for (i=iaa+1; iyvals[i] /= max_aa; dist->dat.yvals = &dist->yvals[irr]; dist->dat.xvals = &dist->xvals[irr]; dist->dat.nvals = iaa - irr + 1; if ( verbose ) fprintf(stderr,"%s:\t cn=%2d \t ra/rr=%f \t aa/ra=%f \t nra=%d\n", dist->chr,dist->copy_number,sra/srr,saa/sra, (int)sra); } static void init_data(args_t *args) { bcf_srs_t *files = bcf_sr_init(); if ( args->regions_list ) { if ( bcf_sr_set_regions(files, args->regions_list, args->regions_is_file)<0 ) error("Failed to read the regions: %s\n", args->regions_list); } if ( args->targets_list ) { if ( bcf_sr_set_targets(files, args->targets_list, args->targets_is_file, 0)<0 ) error("Failed to read the targets: %s\n", args->targets_list); } if ( !bcf_sr_add_reader(files, args->fname) ) error("Failed to open %s: %s\n", args->fname,bcf_sr_strerror(files->errnum)); bcf_hdr_t *hdr = files->readers[0].header; if ( !args->sample ) { if ( bcf_hdr_nsamples(hdr)>1 ) error("Missing the option -s, --sample\n"); args->sample = hdr->samples[0]; } else if ( bcf_hdr_id2int(hdr,BCF_DT_SAMPLE,args->sample)<0 ) error("No such sample: %s\n", args->sample); int ret = bcf_hdr_set_samples(hdr, args->sample, 0); if ( ret<0 ) error("Error setting the sample: %s\n", args->sample); if ( !bcf_hdr_idinfo_exists(hdr,BCF_HL_FMT,bcf_hdr_id2int(hdr,BCF_DT_ID,"BAF")) ) error("The tag FORMAT/BAF is not present in the VCF: %s\n", args->fname); int i; args->xvals = (double*) calloc(args->nbins,sizeof(double)); for (i=0; inbins; i++) args->xvals[i] = 1.0*i/(args->nbins-1); // collect BAF distributions for all chromosomes int idist = -1, nbaf = 0, nprocessed = 0, ntotal = 0, prev_chr = -1; float *baf = NULL; while ( bcf_sr_next_line(files) ) { ntotal++; bcf1_t *line = bcf_sr_get_line(files,0); if ( bcf_get_format_float(hdr,line,"BAF",&baf,&nbaf) != 1 ) continue; if ( bcf_float_is_missing(baf[0]) ) continue; nprocessed++; if ( prev_chr==-1 || prev_chr!=line->rid ) { // new chromosome idist = args->ndist++; args->dist = (dist_t*) realloc(args->dist, sizeof(dist_t)*args->ndist); memset(&args->dist[idist],0,sizeof(dist_t)); args->dist[idist].chr = strdup(bcf_seqname(hdr,line)); args->dist[idist].yvals = (double*) calloc(args->nbins,sizeof(double)); args->dist[idist].xvals = args->xvals; args->dist[idist].nvals = args->nbins; prev_chr = line->rid; } int bin = baf[0]*(args->nbins-1); args->dist[idist].yvals[bin]++; // the distribution } free(baf); bcf_sr_destroy(files); for (idist=0; idistndist; idist++) init_dist(&args->dist[idist],args->verbose); args->dat_fp = open_file(&args->dat_fname,"w","%s/dist.dat", args->output_dir); fprintf(args->dat_fp, "# This file was produced by: bcftools polysomy(%s+htslib-%s), the command line was:\n", bcftools_version(),hts_version()); fprintf(args->dat_fp, "# \t bcftools %s ", args->argv[0]); for (i=1; iargc; i++) fprintf(args->dat_fp, " %s",args->argv[i]); fprintf(args->dat_fp,"\n#\n"); fprintf(args->dat_fp,"# DIST\t[2]Chrom\t[3]BAF\t[4]Normalized Count\n"); fprintf(args->dat_fp,"# FIT\t[2]Chrom\t[3]Mean of fitted Gaussian\t[4]Scale\t[5]Sigma[6]\tMean etc.\n"); fprintf(args->dat_fp,"# CN\t[2]Chrom\t[3]Estimated Copy Number\t[4]Absolute fit deviation\n"); char *fname = NULL; FILE *fp = open_file(&fname,"w","%s/dist.py", args->output_dir); //-------- matplotlib script -------------- fprintf(fp, "#!/usr/bin/env python\n" "#\n" "import matplotlib as mpl\n" "mpl.use('Agg')\n" "import matplotlib.pyplot as plt\n" "import csv,math,sys,argparse\n" "\n" "outdir = '%s'\n" "\n" "def read_dat(dat,fit,cn):\n" " csv.register_dialect('tab', delimiter='\t', quoting=csv.QUOTE_NONE)\n" " with open(outdir+'/dist.dat', 'rb') as f:\n" " reader = csv.reader(f, 'tab')\n" " for row in reader:\n" " if row[0][0]=='#': continue\n" " type = row[0]\n" " chr = row[1]\n" " if type=='DIST':\n" " if chr not in dat: dat[chr] = []\n" " dat[chr].append(row)\n" " elif type=='FIT':\n" " if chr not in fit: fit[chr] = []\n" " fit[chr] = row[2:]\n" " elif type=='CN':\n" " cn[chr] = row[2]\n" "\n" "def fitted_func(xvals,params):\n" " n = len(params)/3\n" " out = []\n" " for x in xvals:\n" " y = 0\n" " for i in range(n):\n" " mean = float(params[i*3+0])\n" " scale = float(params[i*3+1])\n" " sigma = float(params[i*3+2])\n" " y += scale * math.exp(-(float(x)-mean)**2/sigma**2)\n" " out.append(y)\n" " return out\n" "\n" "def plot_dist(dat,fit,chr):\n" " fig, ax = plt.subplots(1, 1, figsize=(7,5))\n" " ax.plot([x[2] for x in dat[chr]],[x[3] for x in dat[chr]],'-',label='Distribution')\n" " ax.plot([x[2] for x in dat[chr]],fitted_func([x[2] for x in dat[chr]], fit[chr]),'-',label='Best Fit')\n" " ax.set_title('BAF distribution, chr'+chr)\n" " ax.set_xlabel('BAF')\n" " ax.set_ylabel('Frequency')\n" " ax.legend(loc='best',prop={'size':7},frameon=False)\n" " plt.savefig(outdir+'/dist.chr'+chr+'.png')\n" " plt.close()\n" "\n" "def plot_copy_number(cn):\n" " fig, ax = plt.subplots(1, 1, figsize=(7,5))\n" " xlabels = sorted(cn.keys())\n" " xvals = range(len(xlabels))\n" " yvals = [float(cn[x]) for x in xlabels]\n" " ax.plot(xvals,yvals,'o',color='red')\n" " for i in range(len(xvals)):\n" " if yvals[i]==-1: ax.annotate('?', xy=(xvals[i],0.5),va='center',ha='center',color='red',fontweight='bold')\n" " ax.tick_params(axis='both', which='major', labelsize=9)\n" " ax.set_xticks(xvals)\n" " ax.set_xticklabels(xlabels,rotation=45)\n" " ax.set_xlim(-1,len(xlabels))\n" " ax.set_ylim(0,5.0)\n" " ax.set_yticks([1.0,2.0,3.0,4.0])\n" " ax.set_xlabel('Chromosome')\n" " ax.set_ylabel('Copy Number')\n" " plt.savefig(outdir+'/copy-number.png')\n" " plt.close()\n" "\n" "def main():\n" " parser = argparse.ArgumentParser()\n" " parser.add_argument('-a', '--all', action='store_true', help='Create all plots')\n" " parser.add_argument('-c', '--copy-number', action='store_true', help='Create copy-number plot')\n" " parser.add_argument('-d', '--distrib', metavar='CHR', help='Plot BAF distribution of a single chromosome')\n" " args = parser.parse_args()\n" " if args.distrib==None and not args.all and not args.copy_number: parser.print_help()\n" " dat = {}; fit = {}; cn = {}\n" " read_dat(dat,fit,cn)\n" " if args.distrib!=None:\n" " plot_dist(dat,fit,args.distrib)\n" " if args.all:\n" " for chr in dat: plot_dist(dat,fit,chr)\n" " plot_copy_number(cn)\n" " elif args.copy_number:\n" " plot_copy_number(cn)\n" "\n" "if __name__ == '__main__':\n" " main()\n", args->output_dir); //--------------------------------------- chmod(fname, S_IWUSR|S_IRUSR|S_IRGRP|S_IROTH|S_IXUSR|S_IXGRP|S_IXOTH); free(fname); fclose(fp); } static void destroy_data(args_t *args) { int i; for (i=0; indist; i++) { free(args->dist[i].chr); free(args->dist[i].yvals); } free(args->dist); free(args->xvals); free(args->dat_fname); fclose(args->dat_fp); } static void save_dist(args_t *args, int idist, int ngauss, double *params) { int i; for (i=0; inbins; i++) fprintf(args->dat_fp,"DIST\t%s\t%f\t%f\n",args->dist[idist].chr,args->dist[idist].xvals[i],args->dist[idist].yvals[i]); fprintf(args->dat_fp,"FIT\t%s", args->dist[idist].chr); for (i=0; idat_fp,"\t%f", params[i]); fprintf(args->dat_fp,"\n"); } int func_f(const gsl_vector *params, void *data, gsl_vector *yvals) { data_t *dat = (data_t *) data; int i, j; for (i=0; invals; i++) { double xi = dat->xvals[i]; double yi = 0; for (j=0; jngauss; j++) { double center = gsl_vector_get(params,j*3 + 0); double scale = gsl_vector_get(params,j*3 + 1); double sigma = gsl_vector_get(params,j*3 + 2); double zi = (xi - center) / sigma; yi += scale*scale * exp(-zi*zi); } gsl_vector_set(yvals, i, (yi - dat->yvals[i])/0.1); } return GSL_SUCCESS; } int func_df(const gsl_vector *params, void *data, gsl_matrix *jacobian) { data_t *dat = (data_t *) data; int i, j; for (i=0; invals; i++) { // Jacobian matrix J(i,j) = dfi / dxj, // where fi = (Yi - yi), // Yi = scale^2 * exp(-(center - xi)^2/sigma^2) // double xi = dat->xvals[i]; for (j=0; jngauss; j++) { double center = gsl_vector_get(params,j*3 + 0); double scale = gsl_vector_get(params,j*3 + 1); double sigma = gsl_vector_get(params,j*3 + 2); double zi = (xi - center) / sigma; double ei = exp(-zi*zi); gsl_matrix_set(jacobian, i, j*3 + 0, 2*scale*scale*(xi-center)/(sigma*sigma)*ei); gsl_matrix_set(jacobian, i, j*3 + 1, 2*scale*ei); gsl_matrix_set(jacobian, i, j*3 + 2, 2*scale*scale*(xi-center)*(xi-center)/(sigma*sigma*sigma)*ei); } } return GSL_SUCCESS; } int func_set(const gsl_vector *params, void *data, gsl_vector *yvals, gsl_matrix *jacobian) { func_f(params, data, yvals); func_df(params, data, jacobian); return GSL_SUCCESS; } static double eval_fit(int nvals, double *xvals, double *yvals, int ngauss, double *params) { double sum = 0; int i, j; for (i=0; idat; dat->ngauss = ngauss; gsl_multifit_function_fdf mfunc; mfunc.f = &func_f; mfunc.df = &func_df; mfunc.fdf = &func_set; mfunc.n = dat->nvals; mfunc.p = ngauss*3; // number of fitting parameters mfunc.params = dat; const gsl_multifit_fdfsolver_type *solver_type; gsl_multifit_fdfsolver *solver; gsl_vector_view vview = gsl_vector_view_array(params, mfunc.p); solver_type = gsl_multifit_fdfsolver_lmsder; solver = gsl_multifit_fdfsolver_alloc(solver_type, dat->nvals, mfunc.p); gsl_multifit_fdfsolver_set(solver, &mfunc, &vview.vector); int i, status; size_t iter = 0; do { status = gsl_multifit_fdfsolver_iterate(solver); if ( status ) break; status = gsl_multifit_test_delta(solver->dx, solver->x, 1e-4, 1e-4); } while (status == GSL_CONTINUE && iter++ < 500); for (i=0; ix, i); gsl_multifit_fdfsolver_free(solver); return iter>500 ? -1 : 0; } static double best_fit(args_t *args, dist_t *dist, int ngauss, double *params) { if ( ngauss==1 ) { gauss_fit(dist,ngauss,params); params[1] *= params[1]; return eval_fit(dist->dat.nvals, dist->dat.xvals, dist->dat.yvals, ngauss,params); } int i, j, n = 3; int ipk = 3*(ngauss-1); double delta = 0.5 * (params[ipk] - params[0]) / n; double best_params[9], tmp_params[9], best_fit = HUGE_VAL; for (i=0; idat.nvals, dist->dat.xvals, dist->dat.yvals, ngauss, tmp_params); if ( best_fit < fit ) continue; // worse than previous best_fit = fit; memcpy(best_params,tmp_params,sizeof(double)*ngauss*3); } memcpy(params,best_params,sizeof(double)*ngauss*3); return best_fit; } static void print_params(data_t *dat, int ngauss, double *params, float fit, float frac, char fail, char comment) { int i, j; printf("\t%c%c fit=%f frac=%.2f .. center,scale,sigma = ", comment,fail?fail:'o',fit,frac); for (i=0; indist; i++) { dist_t *dist = &args->dist[i]; if ( dist->copy_number!=0 ) { fprintf(args->dat_fp,"CN\t%s\t%.2f\n", dist->chr,(float)dist->copy_number); save_dist(args, i, 0, NULL); continue; } // Parameters (center,scale,sigma) for gaussian peaks. double params_cn2[] = { 1/2.,0.5,0.05 }; double params_cn3[] = { 1/3.,0.5,0.05, 2/3.,0.5,0.05 }; double params_cn4[] = { 1/4.,0.5,0.05, 1/2.,0.5,0.05, 3/4.,0.5,0.05 }; double fit_cn2 = best_fit(args,&args->dist[i],1,params_cn2); double fit_cn3 = best_fit(args,&args->dist[i],2,params_cn3); double fit_cn4 = best_fit(args,&args->dist[i],3,params_cn4); double dx_cn3 = fabs(params_cn3[0] - params_cn3[3]); double dx_cn4 = fabs(params_cn4[0] - params_cn4[6]); double dy_cn3 = params_cn3[1] > params_cn3[4] ? params_cn3[4]/params_cn3[1] : params_cn3[1]/params_cn3[4]; double dy_cn4a = params_cn4[1] > params_cn4[7] ? params_cn4[7]/params_cn4[1] : params_cn4[1]/params_cn4[7]; // side peaks double ymax = params_cn4[1] > params_cn4[7] ? params_cn4[1] : params_cn4[7]; double dy_cn4b = ymax > params_cn4[4] ? params_cn4[4]/ymax : ymax/params_cn4[4]; // middle peak // Three peaks (CN4) are always a better fit than two (CN3) or one (CN2). Therefore // check that peaks are well separated and that the peak sizes are reasonable char cn2_fail = 0, cn3_fail = 0, cn4_fail = 0; if ( fit_cn2 > args->fit_th ) cn2_fail = 'f'; if ( fit_cn3 > args->fit_th ) cn3_fail = 'f'; else if ( dx_cn3 < 0.05 ) cn3_fail = 'x'; // peak separation: at least ~10% of cells else if ( dy_cn3 < args->peak_symmetry ) cn3_fail = 'y'; if ( fit_cn4 > args->fit_th ) cn4_fail = 'f'; else if ( dx_cn4 < 0.1 ) cn4_fail = 'x'; // peak separation else if ( dy_cn4a < args->peak_symmetry ) cn4_fail = 'y'; else if ( dy_cn4b < args->peak_symmetry ) cn4_fail = 'Y'; // Estimate fraction of affected cells. For CN4 we estimate // contamination (the fraction of foreign cells), which is more // common than CN4; hence the value is from the interval [0,0.5]. // CN3 .. f = 2*dx/(1-dx) // CN4 .. f = dx dx_cn3 = 2*dx_cn3 / (1-dx_cn3); double cn = -1, fit = fit_cn2; if ( !cn2_fail ) { cn = 2; fit = fit_cn2; } if ( !cn3_fail && fit_cn3 < args->cn_penalty * fit ) { cn = 3; fit = fit_cn3; } if ( !cn4_fail && fit_cn4 < args->cn_penalty * fit ) { cn = 4; fit = fit_cn4; } if ( cn==-1 ) save_dist(args, i, 0, NULL); else if ( cn==2 ) save_dist(args, i, 1, params_cn2); else if ( cn==3 ) { save_dist(args, i, 2, params_cn3); cn = 2 + dx_cn3; } else if ( cn==4 ) { save_dist(args, i, 3, params_cn4); cn = 3 + dx_cn4; } if ( args->verbose ) { printf("%s: \n", args->dist[i].chr); print_params(&args->dist[i].dat, 1, params_cn2, fit_cn2, 1.0, cn2_fail, cn==2 ? '*' : ' '); print_params(&args->dist[i].dat, 2, params_cn3, fit_cn3, dx_cn3, cn3_fail, cn>2 && cn<=3 ? '*' : ' '); print_params(&args->dist[i].dat, 3, params_cn4, fit_cn4, dx_cn4, cn4_fail, cn>3 ? '*' : ' '); printf("\n"); } fprintf(args->dat_fp,"CN\t%s\t%.2f\t%f\n", dist->chr, cn, fit); } } static void usage(args_t *args) { fprintf(stderr, "\n"); fprintf(stderr, "About: Detect number of chromosomal copies from Illumina's B-allele frequency (BAF)\n"); fprintf(stderr, "Usage: bcftools polysomy [OPTIONS] \n"); fprintf(stderr, "General options:\n"); fprintf(stderr, " -o, --output-dir \n"); fprintf(stderr, " -r, --regions restrict to comma-separated list of regions\n"); fprintf(stderr, " -R, --regions-file restrict to regions listed in a file\n"); fprintf(stderr, " -s, --sample sample to analyze\n"); fprintf(stderr, " -t, --targets similar to -r but streams rather than index-jumps\n"); fprintf(stderr, " -T, --targets-file similar to -R but streams rather than index-jumps\n"); fprintf(stderr, " -v, --verbose \n"); fprintf(stderr, "Algorithm options:\n"); fprintf(stderr, " -c, --cn-penalty penalty for increasing CN (smaller more strict) [0.7]\n"); fprintf(stderr, " -f, --fit-th goodness of fit threshold (smaller more strict) [3.0]\n"); fprintf(stderr, " -p, --peak-symmetry peak symmetry threshold (bigger more strict) [0.7]\n"); fprintf(stderr, "\n"); exit(1); } int main_polysomy(int argc, char *argv[]) { args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->argc = argc; args->argv = argv; args->nbins = 150; args->fit_th = 3.0; args->cn_penalty = 0.7; args->peak_symmetry = 0.7; static struct option loptions[] = { {"verbose",0,0,'v'}, {"fit-th",1,0,'f'}, {"cn-penalty",1,0,'c'}, {"peak-symmetry",1,0,'p'}, {"output-dir",1,0,'o'}, {"sample",1,0,'s'}, {"targets",1,0,'t'}, {"targets-file",1,0,'T'}, {"regions",1,0,'r'}, {"regions-file",1,0,'R'}, {0,0,0,0} }; char c, *tmp; while ((c = getopt_long(argc, argv, "h?o:vt:T:r:R:s:f:p:c:",loptions,NULL)) >= 0) { switch (c) { case 'f': args->fit_th = strtod(optarg,&tmp); if ( *tmp ) error("Could not parse: -f %s\n", optarg); break; case 'p': args->peak_symmetry = strtod(optarg,&tmp); if ( *tmp ) error("Could not parse: -p %s\n", optarg); break; case 'c': args->cn_penalty = strtod(optarg,&tmp); if ( *tmp ) error("Could not parse: -c %s\n", optarg); break; case 's': args->sample = optarg; break; case 't': args->targets_list = optarg; break; case 'T': args->targets_list = optarg; args->targets_is_file = 1; break; case 'r': args->regions_list = optarg; break; case 'R': args->regions_list = optarg; args->regions_is_file = 1; break; case 'o': args->output_dir = optarg; break; case 'v': args->verbose = 1; break; default: usage(args); break; } } if ( optind>=argc ) { if ( !isatty(fileno((FILE *)stdin)) ) args->fname = "-"; } else args->fname = argv[optind]; if ( !args->fname ) usage(args); if ( !args->output_dir ) error("Missing the -o option\n"); init_data(args); fit_curves(args); destroy_data(args); free(args); return 0; } bcftools-1.2/prob1.c000066400000000000000000000475031246371514100143730ustar00rootroot00000000000000/* prob1.c -- mathematical utility functions. Copyright (C) 2010, 2011 Broad Institute. Copyright (C) 2012, 2013 Genome Research Ltd. Author: Heng Li Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include "prob1.h" // #include "kstring.h" // #include "kseq.h" // KSTREAM_INIT(gzFile, gzread, 16384) #define MC_MAX_EM_ITER 16 #define MC_EM_EPS 1e-5 #define MC_DEF_INDEL 0.15 gzFile bcf_p1_fp_lk; void bcf_p1_indel_prior(bcf_p1aux_t *ma, double x) { int i; for (i = 0; i < ma->M; ++i) ma->phi_indel[i] = ma->phi[i] * x; ma->phi_indel[ma->M] = 1. - ma->phi[ma->M] * x; } static void init_prior(int type, double theta, int M, double *phi) { int i; if (type == MC_PTYPE_COND2) { for (i = 0; i <= M; ++i) phi[i] = 2. * (i + 1) / (M + 1) / (M + 2); } else if (type == MC_PTYPE_FLAT) { for (i = 0; i <= M; ++i) phi[i] = 1. / (M + 1); } else { double sum; for (i = 0, sum = 0.; i < M; ++i) sum += (phi[i] = theta / (M - i)); phi[M] = 1. - sum; } } void bcf_p1_init_prior(bcf_p1aux_t *ma, int type, double theta) { init_prior(type, theta, ma->M, ma->phi); bcf_p1_indel_prior(ma, MC_DEF_INDEL); } void bcf_p1_init_subprior(bcf_p1aux_t *ma, int type, double theta) { if (ma->n1 <= 0 || ma->n1 >= ma->M) return; init_prior(type, theta, 2*ma->n1, ma->phi1); init_prior(type, theta, 2*(ma->n - ma->n1), ma->phi2); } /* Initialise a bcf_p1aux_t */ bcf_p1aux_t *bcf_p1_init(int n_smpl, uint8_t *ploidy) { bcf_p1aux_t *ma; int i; ma = (bcf_p1aux_t*) calloc(1, sizeof(bcf_p1aux_t)); ma->n1 = -1; ma->n = n_smpl; ma->M = 2 * n_smpl; if (ploidy) { ma->ploidy = (uint8_t*) malloc(n_smpl); memcpy(ma->ploidy, ploidy, n_smpl); for (i = 0, ma->M = 0; i < n_smpl; ++i) ma->M += ploidy[i]; if (ma->M == 2 * n_smpl) { free(ma->ploidy); ma->ploidy = 0; } } ma->q2p = (double*) calloc(256, sizeof(double)); ma->pdg = (double*) calloc(3 * ma->n, sizeof(double)); ma->phi = (double*) calloc(ma->M + 1, sizeof(double)); ma->phi_indel = (double*) calloc(ma->M + 1, sizeof(double)); ma->phi1 = (double*) calloc(ma->M + 1, sizeof(double)); ma->phi2 = (double*) calloc(ma->M + 1, sizeof(double)); ma->z = (double*) calloc(ma->M + 1, sizeof(double)); ma->zswap = (double*) calloc(ma->M + 1, sizeof(double)); ma->z1 = (double*) calloc(ma->M + 1, sizeof(double)); // actually we do not need this large ma->z2 = (double*) calloc(ma->M + 1, sizeof(double)); ma->afs = (double*) calloc(ma->M + 1, sizeof(double)); ma->afs1 = (double*) calloc(ma->M + 1, sizeof(double)); ma->lf = (double*) calloc(ma->M + 1, sizeof(double)); for (i = 0; i < 256; ++i) ma->q2p[i] = pow(10., -i / 10.); for (i = 0; i <= ma->M; ++i) ma->lf[i] = lgamma(i + 1); bcf_p1_init_prior(ma, MC_PTYPE_FULL, 1e-3); // the simplest prior return ma; } int bcf_p1_get_M(bcf_p1aux_t *b) { return b->M; } int bcf_p1_set_n1(bcf_p1aux_t *b, int n1) { if (n1 == 0 || n1 >= b->n) return -1; if (b->M != b->n * 2) { fprintf(stderr, "[%s] unable to set `n1' when there are haploid samples.\n", __func__); return -1; } b->n1 = n1; return 0; } void bcf_p1_destroy(bcf_p1aux_t *ma) { if (ma) { int k; free(ma->lf); if (ma->hg && ma->n1 > 0) { for (k = 0; k <= 2*ma->n1; ++k) free(ma->hg[k]); free(ma->hg); } free(ma->ploidy); free(ma->q2p); free(ma->pdg); free(ma->phi); free(ma->phi_indel); free(ma->phi1); free(ma->phi2); free(ma->z); free(ma->zswap); free(ma->z1); free(ma->z2); free(ma->afs); free(ma->afs1); free(ma); } } extern double kf_gammap(double s, double z); int test16(bcf1_t *b, anno16_t *a); /* Calculate P(D|g) */ static int cal_pdg(const bcf1_t *b, bcf_p1aux_t *ma) { int i, j; long *p, tmp; p = (long*) alloca(b->n_allele * sizeof(long)); memset(p, 0, sizeof(long) * b->n_allele); // Set P(D|g) for each sample and sum phread likelihoods across all samples to create lk for (j = 0; j < ma->n; ++j) { // Fetch the PL array for the sample const int *pi = ma->PL + j * ma->PL_len; // Fetch the P(D|g) array for the sample double *pdg = ma->pdg + j * 3; pdg[0] = ma->q2p[pi[2]]; pdg[1] = ma->q2p[pi[1]]; pdg[2] = ma->q2p[pi[0]]; for (i = 0; i < b->n_allele; ++i) p[i] += (int)pi[(i+1)*(i+2)/2-1]; } for (i = 0; i < b->n_allele; ++i) p[i] = p[i]<<4 | i; for (i = 1; i < b->n_allele; ++i) // insertion sort for (j = i; j > 0 && p[j] < p[j-1]; --j) tmp = p[j], p[j] = p[j-1], p[j-1] = tmp; for (i = b->n_allele - 1; i >= 0; --i) if ((p[i]&0xf) == 0) break; return i; } /* f0 is minor allele fraction */ int bcf_p1_call_gt(const bcf_p1aux_t *ma, double f0, int k) { double sum, g[3]; double max, f3[3], *pdg = ma->pdg + k * 3; int q, i, max_i, ploidy; /* determine ploidy */ ploidy = ma->ploidy? ma->ploidy[k] : 2; if (ploidy == 2) { /* given allele frequency we can determine how many of each * genotype we have by HWE p=1-q PP=p^2 PQ&QP=2*p*q QQ=q^2 */ f3[0] = (1.-f0)*(1.-f0); f3[1] = 2.*f0*(1.-f0); f3[2] = f0*f0; } else { f3[0] = 1. - f0; f3[1] = 0; f3[2] = f0; } for (i = 0, sum = 0.; i < 3; ++i) sum += (g[i] = pdg[i] * f3[i]); /* normalise g and then determine max */ for (i = 0, max = -1., max_i = 0; i < 3; ++i) { g[i] /= sum; if (g[i] > max) max = g[i], max_i = i; } max = 1. - max; if (max < 1e-308) max = 1e-308; q = (int)(-4.343 * log(max) + .499); if (q > 99) q = 99; return q<<2|max_i; } // If likelihoods fall below this they get squashed to 0 #define TINY 1e-20 static void mc_cal_y_core(bcf_p1aux_t *ma, int beg) { double *z[2], *tmp, *pdg; int _j, last_min, last_max; assert(beg == 0 || ma->M == ma->n*2); z[0] = ma->z; z[1] = ma->zswap; pdg = ma->pdg; memset(z[0], 0, sizeof(double) * (ma->M + 1)); memset(z[1], 0, sizeof(double) * (ma->M + 1)); z[0][0] = 1.; last_min = last_max = 0; ma->t = 0.; if (ma->M == ma->n * 2) { int M = 0; for (_j = beg; _j < ma->n; ++_j) { int k, j = _j - beg, _min = last_min, _max = last_max, M0; double p[3], sum; M0 = M; M += 2; // Fetch P(D|g) for this sample pdg = ma->pdg + _j * 3; p[0] = pdg[0]; p[1] = 2. * pdg[1]; p[2] = pdg[2]; for (; _min < _max && z[0][_min] < TINY; ++_min) z[0][_min] = z[1][_min] = 0.; for (; _max > _min && z[0][_max] < TINY; --_max) z[0][_max] = z[1][_max] = 0.; _max += 2; if (_min == 0) k = 0, z[1][k] = (M0-k+1) * (M0-k+2) * p[0] * z[0][k]; if (_min <= 1) k = 1, z[1][k] = (M0-k+1) * (M0-k+2) * p[0] * z[0][k] + k*(M0-k+2) * p[1] * z[0][k-1]; for (k = _min < 2? 2 : _min; k <= _max; ++k) z[1][k] = (M0-k+1)*(M0-k+2) * p[0] * z[0][k] + k*(M0-k+2) * p[1] * z[0][k-1] + k*(k-1)* p[2] * z[0][k-2]; for (k = _min, sum = 0.; k <= _max; ++k) sum += z[1][k]; ma->t += log(sum / (M * (M - 1.))); for (k = _min; k <= _max; ++k) z[1][k] /= sum; if (_min >= 1) z[1][_min-1] = 0.; if (_min >= 2) z[1][_min-2] = 0.; // If we are not on the last sample if (j < ma->n - 1) z[1][_max+1] = z[1][_max+2] = 0.; if (_j == ma->n1 - 1) { // set pop1; ma->n1==-1 when unset ma->t1 = ma->t; memcpy(ma->z1, z[1], sizeof(double) * (ma->n1 * 2 + 1)); } tmp = z[0]; z[0] = z[1]; z[1] = tmp; last_min = _min; last_max = _max; } //for (_j = 0; _j < last_min; ++_j) z[0][_j] = 0.; // TODO: are these necessary? //for (_j = last_max + 1; _j < ma->M; ++_j) z[0][_j] = 0.; } else { // this block is very similar to the block above; these two might be merged in future int j, M = 0; for (j = 0; j < ma->n; ++j) { int k, M0, _min = last_min, _max = last_max; double p[3], sum; // Fetch P(D|g) for this sample pdg = ma->pdg + j * 3; for (; _min < _max && z[0][_min] < TINY; ++_min) z[0][_min] = z[1][_min] = 0.; for (; _max > _min && z[0][_max] < TINY; --_max) z[0][_max] = z[1][_max] = 0.; M0 = M; M += ma->ploidy[j]; if (ma->ploidy[j] == 1) { p[0] = pdg[0]; p[1] = pdg[2]; _max++; if (_min == 0) k = 0, z[1][k] = (M0+1-k) * p[0] * z[0][k]; for (k = _min < 1? 1 : _min; k <= _max; ++k) z[1][k] = (M0+1-k) * p[0] * z[0][k] + k * p[1] * z[0][k-1]; for (k = _min, sum = 0.; k <= _max; ++k) sum += z[1][k]; ma->t += log(sum / M); for (k = _min; k <= _max; ++k) z[1][k] /= sum; if (_min >= 1) z[1][_min-1] = 0.; // If we are not on the last sample if (j < ma->n - 1) z[1][_max+1] = 0.; } else if (ma->ploidy[j] == 2) { p[0] = pdg[0]; p[1] = 2 * pdg[1]; p[2] = pdg[2]; _max += 2; if (_min == 0) k = 0, z[1][k] = (M0-k+1) * (M0-k+2) * p[0] * z[0][k]; if (_min <= 1) k = 1, z[1][k] = (M0-k+1) * (M0-k+2) * p[0] * z[0][k] + k*(M0-k+2) * p[1] * z[0][k-1]; for (k = _min < 2? 2 : _min; k <= _max; ++k) z[1][k] = (M0-k+1)*(M0-k+2) * p[0] * z[0][k] + k*(M0-k+2) * p[1] * z[0][k-1] + k*(k-1)* p[2] * z[0][k-2]; for (k = _min, sum = 0.; k <= _max; ++k) sum += z[1][k]; ma->t += log(sum / (M * (M - 1.))); for (k = _min; k <= _max; ++k) z[1][k] /= sum; if (_min >= 1) z[1][_min-1] = 0.; if (_min >= 2) z[1][_min-2] = 0.; // If we are not on the last sample if (j < ma->n - 1) z[1][_max+1] = z[1][_max+2] = 0.; } tmp = z[0]; z[0] = z[1]; z[1] = tmp; last_min = _min; last_max = _max; } } if (z[0] != ma->z) memcpy(ma->z, z[0], sizeof(double) * (ma->M + 1)); if (bcf_p1_fp_lk) gzwrite(bcf_p1_fp_lk, ma->z, sizeof(double) * (ma->M + 1)); } static void mc_cal_y(bcf_p1aux_t *ma) { if (ma->n1 > 0 && ma->n1 < ma->n && ma->M == ma->n * 2) { // NB: ma->n1 is ineffective when there are haploid samples int k; long double x; memset(ma->z1, 0, sizeof(double) * (2 * ma->n1 + 1)); memset(ma->z2, 0, sizeof(double) * (2 * (ma->n - ma->n1) + 1)); ma->t1 = ma->t2 = 0.; mc_cal_y_core(ma, ma->n1); ma->t2 = ma->t; memcpy(ma->z2, ma->z, sizeof(double) * (2 * (ma->n - ma->n1) + 1)); mc_cal_y_core(ma, 0); // rescale z x = expl(ma->t - (ma->t1 + ma->t2)); for (k = 0; k <= ma->M; ++k) ma->z[k] *= x; } else mc_cal_y_core(ma, 0); } #define CONTRAST_TINY 1e-30 extern double kf_gammaq(double s, double z); // incomplete gamma function for chi^2 test static inline double chi2_test(int a, int b, int c, int d) { double x, z; x = (double)(a+b) * (c+d) * (b+d) * (a+c); if (x == 0.) return 1; z = a * d - b * c; return kf_gammaq(.5, .5 * z * z * (a+b+c+d) / x); } // chi2=(a+b+c+d)(ad-bc)^2/[(a+b)(c+d)(a+c)(b+d)] static inline double contrast2_aux(const bcf_p1aux_t *p1, double sum, int k1, int k2, double x[3]) { double p = p1->phi[k1+k2] * p1->z1[k1] * p1->z2[k2] / sum * p1->hg[k1][k2]; int n1 = p1->n1, n2 = p1->n - p1->n1; if (p < CONTRAST_TINY) return -1; if (.5*k1/n1 < .5*k2/n2) x[1] += p; else if (.5*k1/n1 > .5*k2/n2) x[2] += p; else x[0] += p; return p * chi2_test(k1, k2, (n1<<1) - k1, (n2<<1) - k2); } static double contrast2(bcf_p1aux_t *p1, double ret[3]) { int k, k1, k2, k10, k20, n1, n2; double sum; // get n1 and n2 n1 = p1->n1; n2 = p1->n - p1->n1; if (n1 <= 0 || n2 <= 0) return 0.; if (p1->hg == 0) { // initialize the hypergeometric distribution /* NB: the hg matrix may take a lot of memory when there are many samples. There is a way to avoid precomputing this matrix, but it is slower and quite intricate. The following computation in this block can be accelerated with a similar strategy, but perhaps this is not a serious concern for now. */ double tmp = lgamma(2*(n1+n2)+1) - (lgamma(2*n1+1) + lgamma(2*n2+1)); p1->hg = (double**) calloc(2*n1+1, sizeof(double*)); for (k1 = 0; k1 <= 2*n1; ++k1) { p1->hg[k1] = (double*)calloc(2*n2+1, sizeof(double)); for (k2 = 0; k2 <= 2*n2; ++k2) p1->hg[k1][k2] = exp(lgamma(k1+k2+1) + lgamma(p1->M-k1-k2+1) - (lgamma(k1+1) + lgamma(k2+1) + lgamma(2*n1-k1+1) + lgamma(2*n2-k2+1) + tmp)); } } { // compute long double suml = 0; for (k = 0; k <= p1->M; ++k) suml += p1->phi[k] * p1->z[k]; sum = suml; } { // get the max k1 and k2 double max; int max_k; for (k = 0, max = 0, max_k = -1; k <= 2*n1; ++k) { double x = p1->phi1[k] * p1->z1[k]; if (x > max) max = x, max_k = k; } k10 = max_k; for (k = 0, max = 0, max_k = -1; k <= 2*n2; ++k) { double x = p1->phi2[k] * p1->z2[k]; if (x > max) max = x, max_k = k; } k20 = max_k; } { // We can do the following with one nested loop, but that is an O(N^2) thing. The following code block is much faster for large N. double x[3], y; long double z = 0., L[2]; x[0] = x[1] = x[2] = 0; L[0] = L[1] = 0; for (k1 = k10; k1 >= 0; --k1) { for (k2 = k20; k2 >= 0; --k2) { if ((y = contrast2_aux(p1, sum, k1, k2, x)) < 0) break; else z += y; } for (k2 = k20 + 1; k2 <= 2*n2; ++k2) { if ((y = contrast2_aux(p1, sum, k1, k2, x)) < 0) break; else z += y; } } ret[0] = x[0]; ret[1] = x[1]; ret[2] = x[2]; x[0] = x[1] = x[2] = 0; for (k1 = k10 + 1; k1 <= 2*n1; ++k1) { for (k2 = k20; k2 >= 0; --k2) { if ((y = contrast2_aux(p1, sum, k1, k2, x)) < 0) break; else z += y; } for (k2 = k20 + 1; k2 <= 2*n2; ++k2) { if ((y = contrast2_aux(p1, sum, k1, k2, x)) < 0) break; else z += y; } } ret[0] += x[0]; ret[1] += x[1]; ret[2] += x[2]; if (ret[0] + ret[1] + ret[2] < 0.95) { // in case of bad things happened ret[0] = ret[1] = ret[2] = 0; L[0] = L[1] = 0; for (k1 = 0, z = 0.; k1 <= 2*n1; ++k1) for (k2 = 0; k2 <= 2*n2; ++k2) if ((y = contrast2_aux(p1, sum, k1, k2, ret)) >= 0) z += y; if (ret[0] + ret[1] + ret[2] < 0.95) // It seems that this may be caused by floating point errors. I do not really understand why... z = 1.0, ret[0] = ret[1] = ret[2] = 1./3; } return (double)z; } } static double mc_cal_afs(bcf_p1aux_t *ma, double *p_ref_folded, double *p_var_folded) { int k; long double sum = 0., sum2; double *phi = ma->is_indel? ma->phi_indel : ma->phi; memset(ma->afs1, 0, sizeof(double) * (ma->M + 1)); mc_cal_y(ma); // compute AFS // MP15: is this using equation 20 from doi:10.1093/bioinformatics/btr509? for (k = 0, sum = 0.; k <= ma->M; ++k) sum += (long double)phi[k] * ma->z[k]; for (k = 0; k <= ma->M; ++k) { ma->afs1[k] = phi[k] * ma->z[k] / sum; if (isnan(ma->afs1[k]) || isinf(ma->afs1[k])) return -1.; } // compute folded variant probability for (k = 0, sum = 0.; k <= ma->M; ++k) sum += (long double)(phi[k] + phi[ma->M - k]) / 2. * ma->z[k]; for (k = 1, sum2 = 0.; k < ma->M; ++k) sum2 += (long double)(phi[k] + phi[ma->M - k]) / 2. * ma->z[k]; *p_var_folded = sum2 / sum; *p_ref_folded = (phi[k] + phi[ma->M - k]) / 2. * (ma->z[ma->M] + ma->z[0]) / sum; // the expected frequency for (k = 0, sum = 0.; k <= ma->M; ++k) { ma->afs[k] += ma->afs1[k]; sum += k * ma->afs1[k]; } return sum / ma->M; } int bcf_p1_cal(call_t *call, bcf1_t *b, int do_contrast, bcf_p1aux_t *ma, bcf_p1rst_t *rst) { int i, k; long double sum = 0.; ma->is_indel = bcf_is_snp(b) ? 0 : 1; rst->perm_rank = -1; ma->PL = call->PLs; ma->PL_len = call->nPLs / b->n_sample; if (b->n_allele < 2) return -1; // FIXME: find a better solution rst->rank0 = cal_pdg(b, ma); rst->f_exp = mc_cal_afs(ma, &rst->p_ref_folded, &rst->p_var_folded); rst->p_ref = ma->afs1[ma->M]; for (k = 0, sum = 0.; k < ma->M; ++k) sum += ma->afs1[k]; rst->p_var = (double)sum; { // compute the allele count double max = -1; rst->ac = -1; for (k = 0; k <= ma->M; ++k) if (max < ma->z[k]) max = ma->z[k], rst->ac = k; rst->ac = ma->M - rst->ac; } // calculate f_flat and f_em for (k = 0, sum = 0.; k <= ma->M; ++k) sum += (long double)ma->z[k]; rst->f_flat = 0.; for (k = 0; k <= ma->M; ++k) { double p = ma->z[k] / sum; rst->f_flat += k * p; } rst->f_flat /= ma->M; { // estimate equal-tail credible interval (95% level) int l, h; double p; for (i = 0, p = 0.; i <= ma->M; ++i) if (p + ma->afs1[i] > 0.025) break; else p += ma->afs1[i]; l = i; for (i = ma->M, p = 0.; i >= 0; --i) if (p + ma->afs1[i] > 0.025) break; else p += ma->afs1[i]; h = i; rst->cil = (double)(ma->M - h) / ma->M; rst->cih = (double)(ma->M - l) / ma->M; } if (ma->n1 > 0) { // compute LRT double max0, max1, max2; for (k = 0, max0 = -1; k <= ma->M; ++k) if (max0 < ma->z[k]) max0 = ma->z[k]; for (k = 0, max1 = -1; k <= ma->n1 * 2; ++k) if (max1 < ma->z1[k]) max1 = ma->z1[k]; for (k = 0, max2 = -1; k <= ma->M - ma->n1 * 2; ++k) if (max2 < ma->z2[k]) max2 = ma->z2[k]; rst->lrt = log(max1 * max2 / max0); rst->lrt = rst->lrt < 0? 1 : kf_gammaq(.5, rst->lrt); } else rst->lrt = -1.0; rst->cmp[0] = rst->cmp[1] = rst->cmp[2] = rst->p_chi2 = -1.0; if (do_contrast && rst->p_var > 0.5) // skip contrast2() if the locus is a strong non-variant rst->p_chi2 = contrast2(ma, rst->cmp); return 0; } void bcf_p1_dump_afs(bcf_p1aux_t *ma) { int k; fprintf(stderr, "[afs]"); for (k = 0; k <= ma->M; ++k) fprintf(stderr, " %d:%.3lf", k, ma->afs[ma->M - k]); fprintf(stderr, "\n"); memset(ma->afs, 0, sizeof(double) * (ma->M + 1)); } bcftools-1.2/prob1.h000066400000000000000000000067231246371514100143770ustar00rootroot00000000000000/* prob1.h -- mathematical utility functions. Copyright (C) 2010, 2011 Broad Institute. Copyright (C) 2012, 2013 Genome Research Ltd. Author: Heng Li Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #ifndef BCF_PROB1_H #define BCF_PROB1_H #include #include "call.h" typedef struct { int n; // Number of samples int M; // Total number of chromosomes across all samples (n*2 if all samples are diploid) int n1; int is_indel; uint8_t *ploidy; // haploid or diploid ONLY double *q2p, *pdg; // q2p maps from phread scaled to real likelihood, pdg -> P(D|g) double *phi; // Probability of seeing k reference alleles double *phi_indel; double *z, *zswap; // aux for afs double *z1, *z2, *phi1, *phi2; // only calculated when n1 is set double **hg; // hypergeometric distribution double *lf; // log factorial double t, t1, t2; double *afs, *afs1; // afs: accumulative allele frequency spectrum (AFS); afs1: site posterior distribution const int *PL; // point to PL int PL_len; int cons_llr; // pair and trio calling int64_t cons_gt; } bcf_p1aux_t; typedef struct { int rank0, perm_rank; // NB: perm_rank is always set to -1 by bcf_p1_cal() int ac; // ML alternative allele count double f_exp, f_flat, p_ref_folded, p_ref, p_var_folded, p_var; double cil, cih; double cmp[3], p_chi2, lrt; // used by contrast2() } bcf_p1rst_t; typedef struct { double p[4]; double edb, mqb, bqb; // end distance bias, mapQ bias, baseQ bias int mq, depth, is_tested, d[4]; } anno16_t; #define MC_PTYPE_FULL 1 #define MC_PTYPE_COND2 2 #define MC_PTYPE_FLAT 3 #ifdef __cplusplus extern "C" { #endif bcf_p1aux_t *bcf_p1_init(int n_smpl, uint8_t *ploidy); void bcf_p1_init_prior(bcf_p1aux_t *ma, int type, double theta); void bcf_p1_init_subprior(bcf_p1aux_t *ma, int type, double theta); void bcf_p1_destroy(bcf_p1aux_t *ma); void bcf_p1_set_ploidy(bcf1_t *b, bcf_p1aux_t *ma); int bcf_p1_cal(call_t *call, bcf1_t *b, int do_contrast, bcf_p1aux_t *ma, bcf_p1rst_t *rst); int bcf_p1_call_gt(const bcf_p1aux_t *ma, double f0, int k); void bcf_p1_dump_afs(bcf_p1aux_t *ma); int bcf_p1_read_prior(bcf_p1aux_t *ma, const char *fn); int bcf_p1_set_n1(bcf_p1aux_t *b, int n1); void bcf_p1_set_folded(bcf_p1aux_t *p1a); // only effective when set_n1() is not called int bcf_em1(call_t *call, const bcf1_t *b, int n1, int flag, double x[10]); #ifdef __cplusplus } #endif #endif bcftools-1.2/rbuf.h000066400000000000000000000131371246371514100143070ustar00rootroot00000000000000/* rbuf.h -- round buffers. Copyright (C) 2013-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #ifndef __RBUF_H__ #define __RBUF_H__ #include typedef struct { int m,n,f; // m: allocated size, n: number of elements in the buffer, f: first element } rbuf_t; /** * rbuf_init() - initialize round buffer * @rbuf: the rbuf_t holder * @size: the maximum number of elements * */ static inline void rbuf_init(rbuf_t *rbuf, int size) { rbuf->m = size; rbuf->n = rbuf->f = 0; } /** * rbuf_kth() - get index of the k-th element of the round buffer * @rbuf: the rbuf_t holder * @k: 0-based index */ static inline int rbuf_kth(rbuf_t *rbuf, int k) { if ( k >= rbuf->n || k<0 ) return -1; int i = k + rbuf->f; if ( i >= rbuf->m ) i -= rbuf->m; return i; } /** * rbuf_last() - get index of the last element of the round buffer * @rbuf: the rbuf_t holder * */ #define rbuf_last(rbuf) rbuf_kth(rbuf, (rbuf)->n - 1) /** * rbuf_next() - get index of the next element in the round buffer * @rbuf: the rbuf_t holder * @i: pointer to the last rbuf index. Set to -1 before the first call. * * Sets i to the next position in the buffer. The return value indicates if * the position points to a valid element (1) or if there are no more elements * after *i (0). When the end is reached, *i is set to the first element in the * buffer. */ static inline int rbuf_next(rbuf_t *rbuf, int *i) { if ( !rbuf->n ) return 0; if ( *i==-1 ) { *i = rbuf->f; return 1; } int n = (rbuf->f <= *i) ? *i - rbuf->f + 1 : *i + rbuf->m - rbuf->f + 1; if ( ++(*i) >= rbuf->m ) *i = 0; if ( n < rbuf->n ) return 1; *i = rbuf->f; return 0; } /** * rbuf_prev() - get index of the previous element in the round buffer * @rbuf: the rbuf_t holder * @i: pointer to the last rbuf index. Set to -1 before the first call. * * Sets i to the previous position in the buffer. The return value indicates if * the position points to a valid element (1) or if there are no more elements * before *i (0). */ static inline int rbuf_prev(rbuf_t *rbuf, int *i) { if ( !rbuf->n || *i==rbuf->f ) return 0; if ( *i==-1 ) { *i = rbuf_last(rbuf); return 1; } if ( --(*i) < 0 ) *i = rbuf->m - 1; return 1; } /** * rbuf_prepend() - register new element at the start of the round buffer * @rbuf: the rbuf_t holder * * Returns index of the newly inserted element. */ static inline int rbuf_prepend(rbuf_t *rbuf) { if ( rbuf->n < rbuf->m ) rbuf->n++; rbuf->f = rbuf->f > 0 ? rbuf->f - 1 : rbuf->m - 1; return rbuf->f; } /** * rbuf_append() - register new element at the end of the round buffer * @rbuf: the rbuf_t holder * * Returns index of the newly inserted element. */ static inline int rbuf_append(rbuf_t *rbuf) { if ( rbuf->n < rbuf->m ) { rbuf->n++; int i = rbuf->f + rbuf->n; return i <= rbuf->m ? i - 1 : i - rbuf->m - 1; } rbuf->f++; if ( rbuf->f >= rbuf->m ) { rbuf->f = 0; return rbuf->m - 1; } return rbuf->f - 1; } /** * rbuf_shift() - removes first element from the buffer * @rbuf: the rbuf_t holder * * Returns index of the removed element. */ static inline int rbuf_shift(rbuf_t *rbuf) { if ( !rbuf->n ) return -1; int ret = rbuf->f; rbuf->f++; if ( rbuf->f >= rbuf->m ) rbuf->f = 0; rbuf->n--; return ret; } /** * rbuf_shift_n() - removes first n elements from the buffer * @rbuf: the rbuf_t holder * @n: number of elements to remove */ static inline void rbuf_shift_n(rbuf_t *rbuf, int n) { if ( n >= rbuf->n ) { rbuf->n = rbuf->f = 0; return; } rbuf->n -= n; rbuf->f += n; if ( rbuf->f >= rbuf->m ) rbuf->f -= rbuf->m; } /** * rbuf_expand0() - expand round buffer and set the newly allocated elements to 0 * @rbuf: the rbuf holder * @type_t: data type * @data: data array to be realloced * * Note: The new array is linearized and leaves the rbuf.f offset untouched, * thus the size of the new buffer is determined by the current position. */ #define rbuf_expand0(rbuf,type_t,data) { \ int m = (rbuf)->m + (rbuf)->f + 1; \ m--, m|=m>>1, m|=m>>2, m|=m>>4, m|=m>>8, m|=m>>16, m++; /* kroundup32 */ \ data = (type_t*) realloc(data, sizeof(type_t)*m); \ type_t *ptr = data; \ memset(ptr+(rbuf)->m,0,sizeof(type_t)*(m-(rbuf)->m)); \ if ( (rbuf)->f ) \ { \ memcpy(ptr+(rbuf)->m,ptr,sizeof(type_t)*(rbuf)->f); \ memset(ptr,0,sizeof(type_t)*(rbuf)->f); \ } \ (rbuf)->m = m; \ } #endif bcftools-1.2/reheader.c000066400000000000000000000407171246371514100151270ustar00rootroot00000000000000/* reheader.c -- reheader subcommand. Copyright (C) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include #include #include #include #include "bcftools.h" #include "khash_str2str.h" typedef struct _args_t { char **argv, *fname, *samples_fname, *header_fname, *output_fname; htsFile *fp; htsFormat type; int argc; } args_t; static void read_header_file(char *fname, kstring_t *hdr) { kstring_t tmp = {0,0,0}; hdr->l = 0; htsFile *fp = hts_open(fname, "r"); if ( !fp ) error("Could not read: %s\n", fname); while ( hts_getline(fp, KS_SEP_LINE, &tmp) > 0 ) { kputsn(tmp.s,tmp.l,hdr); kputc('\n',hdr); } if ( hts_close(fp) ) error("Close failed: %s\n", fname); free(tmp.s); while ( hdr->l>0 && isspace(hdr->s[hdr->l-1]) ) hdr->l--; // remove trailing newlines kputc('\n',hdr); } static int set_sample_pairs(char **samples, int nsamples, kstring_t *hdr, int idx) { int i, j, n; // Are these samples "old-name new-name" pairs? void *hash = khash_str2str_init(); for (i=0; il>0 && isspace(hdr->s[hdr->l-1]) ) hdr->l--; // remove trailing newlines hdr->s[hdr->l] = 0; kstring_t tmp = {0,0,0}; i = j = n = 0; while ( hdr->s[idx+i] && hdr->s[idx+i]) { if ( hdr->s[idx+i]=='\t' ) { hdr->s[idx+i] = 0; if ( ++n>9 ) { char *ori = khash_str2str_get(hash,hdr->s+idx+j); kputs(ori ? ori : hdr->s+idx+j, &tmp); } else kputs(hdr->s+idx+j, &tmp); kputc('\t',&tmp); j = ++i; continue; } i++; } char *ori = khash_str2str_get(hash,hdr->s+idx+j); kputs(ori ? ori : hdr->s+idx+j, &tmp); if ( hash ) khash_str2str_destroy(hash); hdr->l = idx; kputs(tmp.s, hdr); kputc('\n', hdr); free(tmp.s); return 1; } static void set_samples(char **samples, int nsamples, kstring_t *hdr) { // Find the beginning of the #CHROM line int i = hdr->l - 2, ncols = 0; while ( i>=0 && hdr->s[i]!='\n' ) { if ( hdr->s[i]=='\t' ) ncols++; i--; } if ( i<0 || strncmp(hdr->s+i+1,"#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO\tFORMAT",45) ) error("Could not parse the header: %s\n", hdr->s); // Are the samples "old-sample new-sample" pairs? if ( set_sample_pairs(samples,nsamples,hdr, i+1) ) return; // Replace all samples if ( ncols!=nsamples+8 ) fprintf(stderr, "Warning: different number of samples: %d vs %d\n", nsamples,ncols-8); ncols = 0; while ( ncols!=9 ) { i++; if ( hdr->s[i]=='\t' ) ncols++; } hdr->l = i; for (i=0; ifp); if ( !fp || bgzf_read_block(fp) != 0 || !fp->block_length ) error("Failed to read %s: %s\n", args->fname, strerror(errno)); kstring_t hdr = {0,0,0}; char *buffer = (char*) fp->uncompressed_block; // Read the header and find the position of the data block if ( buffer[0]!='#' ) error("Could not parse the header, expected '#', found '%c'\n", buffer[0]); int skip_until = 1; // end of the header in the current uncompressed block while (1) { if ( buffer[skip_until]=='\n' ) { skip_until++; if ( skip_until>=fp->block_length ) { kputsn(buffer,skip_until,&hdr); if ( bgzf_read_block(fp) != 0 || !fp->block_length ) error("FIXME: No body in the file: %s\n", args->fname); skip_until = 0; } // The header has finished if ( buffer[skip_until]!='#' ) { kputsn(buffer,skip_until,&hdr); break; } } skip_until++; if ( skip_until>=fp->block_length ) { kputsn(buffer,fp->block_length,&hdr); if (bgzf_read_block(fp) != 0 || !fp->block_length) error("FIXME: No body in the file: %s\n", args->fname); skip_until = 0; } } int nsamples = 0; char **samples = NULL; if ( args->samples_fname ) samples = hts_readlines(args->samples_fname, &nsamples); if ( args->header_fname ) { free(hdr.s); hdr.s = NULL; hdr.l = hdr.m = 0; read_header_file(args->header_fname, &hdr); } if ( samples ) { set_samples(samples, nsamples, &hdr); int i; for (i=0; ioutput_fname ) bgzf_out = bgzf_open(args->output_fname,"w"); else bgzf_out = bgzf_dopen(fileno(stdout), "w"); bgzf_write(bgzf_out, hdr.s, hdr.l); free(hdr.s); // Output all remainig data read with the header block if ( fp->block_length - skip_until > 0 ) { if ( bgzf_write(bgzf_out, buffer+skip_until, fp->block_length-skip_until)<0 ) error("Error: %d\n",fp->errcode); } if ( bgzf_flush(bgzf_out)<0 ) error("Error: %d\n",bgzf_out->errcode); // Stream the rest of the file without as it is, without decompressing ssize_t nread; int page_size = getpagesize(); char *buf = (char*) valloc(page_size); while (1) { nread = bgzf_raw_read(fp, buf, page_size); if ( nread<=0 ) break; int count = bgzf_raw_write(bgzf_out, buf, nread); if (count != nread) error("Write failed, wrote %d instead of %d bytes.\n", count,(int)nread); } if (bgzf_close(bgzf_out) < 0) error("Error: %d\n",bgzf_out->errcode); if (bgzf_close(fp) < 0) error("Error: %d\n",fp->errcode); free(buf); } static void reheader_vcf(args_t *args) { kstring_t hdr = {0,0,0}; htsFile *fp = args->fp; while ( hts_getline(fp, KS_SEP_LINE, &fp->line) >=0 ) { kputc('\n',&fp->line); // hts_getline eats the newline character if ( fp->line.s[0]!='#' ) break; kputsn(fp->line.s,fp->line.l,&hdr); } int nsamples = 0; char **samples = NULL; if ( args->samples_fname ) samples = hts_readlines(args->samples_fname, &nsamples); if ( args->header_fname ) { free(hdr.s); hdr.s = NULL; hdr.l = hdr.m = 0; read_header_file(args->header_fname, &hdr); } if ( samples ) { set_samples(samples, nsamples, &hdr); int i; for (i=0; ioutput_fname ? open(args->output_fname, O_WRONLY|O_CREAT|O_TRUNC, 0666) : STDOUT_FILENO; if ( out==-1 ) error("%s: %s\n", args->output_fname,strerror(errno)); if ( write(out, hdr.s, hdr.l)!=hdr.l ) error("Failed to write %d bytes\n", hdr.l); free(hdr.s); if ( fp->line.l ) { if ( write(out, fp->line.s, fp->line.l)!=fp->line.l ) error("Failed to write %d bytes\n", fp->line.l); } while ( hts_getline(fp, KS_SEP_LINE, &fp->line) >=0 ) // uncompressed file implies small size, we don't worry about speed { kputc('\n',&fp->line); if ( write(out, fp->line.s, fp->line.l)!=fp->line.l ) error("Failed to write %d bytes\n", fp->line.l); } hts_close(fp); close(out); } static bcf_hdr_t *strip_header(bcf_hdr_t *src, bcf_hdr_t *dst) { bcf_hrec_t *src_hrec, *dst_hrec, *tmp; bcf_hdr_t *out = bcf_hdr_init("r"); int i; for (i=0; inhrec; i++) { // first insert lines which do not code BCF ids, their order does not matter dst_hrec = dst->hrec[i]; if ( dst_hrec->type==BCF_HL_FLT || dst_hrec->type==BCF_HL_INFO || dst_hrec->type==BCF_HL_FMT || dst_hrec->type== BCF_HL_CTG ) continue; bcf_hdr_add_hrec(out, bcf_hrec_dup(dst_hrec)); } for (i=0; inhrec; i++) { // now transfer header lines which define BCF ids src_hrec = src->hrec[i]; if ( src_hrec->type==BCF_HL_FLT || src_hrec->type==BCF_HL_INFO || src_hrec->type==BCF_HL_FMT || src_hrec->type== BCF_HL_CTG ) { int j = bcf_hrec_find_key(src_hrec, "ID"); dst_hrec = bcf_hdr_get_hrec(dst, src_hrec->type, "ID", src_hrec->vals[j], NULL); if ( !dst_hrec ) continue; tmp = bcf_hrec_dup(dst_hrec); j = bcf_hrec_find_key(src_hrec, "IDX"); if ( j>=0 ) { j = atoi(src_hrec->vals[j]); hrec_add_idx(tmp, j); } bcf_hdr_add_hrec(out, tmp); } } bcf_hdr_sync(out); for (i=0; inhrec; i++) { // finally add new structured fields dst_hrec = dst->hrec[i]; if ( dst_hrec->type==BCF_HL_FLT || dst_hrec->type==BCF_HL_INFO || dst_hrec->type==BCF_HL_FMT || dst_hrec->type== BCF_HL_CTG ) { int j = bcf_hrec_find_key(dst_hrec, "ID"); tmp = bcf_hdr_get_hrec(out, dst_hrec->type, "ID", dst_hrec->vals[j], NULL); if ( !tmp ) bcf_hdr_add_hrec(out, bcf_hrec_dup(dst_hrec)); } } for (i=0; in[BCF_DT_SAMPLE]; i++) bcf_hdr_add_sample(out, dst->samples[i]); bcf_hdr_destroy(dst); return out; } static void reheader_bcf(args_t *args, int is_compressed) { htsFile *fp = args->fp; bcf_hdr_t *hdr = bcf_hdr_read(fp); if ( !hdr ) error("Failed to read the header: %s\n", args->fname); kstring_t htxt = {0,0,0}; int hlen; htxt.s = bcf_hdr_fmt_text(hdr, 1, &hlen); htxt.l = hlen; int i, nsamples = 0; char **samples = NULL; if ( args->samples_fname ) samples = hts_readlines(args->samples_fname, &nsamples); if ( args->header_fname ) { free(htxt.s); htxt.s = NULL; htxt.l = htxt.m = 0; read_header_file(args->header_fname, &htxt); } if ( samples ) { set_samples(samples, nsamples, &htxt); for (i=0; iheader_fname ) hdr_out = strip_header(hdr, hdr_out); // write the header and the body htsFile *fp_out = hts_open(args->output_fname ? args->output_fname : "-",is_compressed ? "wb" : "wbu"); if ( !fp_out ) error("%s: %s\n", args->output_fname ? args->output_fname : "-", strerror(errno)); bcf_hdr_write(fp_out, hdr_out); bcf1_t *rec = bcf_init(); while ( bcf_read(fp, hdr, rec)==0 ) { // sanity checking, this slows things down. Make it optional? bcf_unpack(rec, BCF_UN_ALL); if ( rec->rid >= hdr_out->n[BCF_DT_CTG] || strcmp(bcf_hdr_int2id(hdr,BCF_DT_CTG,rec->rid),bcf_hdr_int2id(hdr_out,BCF_DT_CTG,rec->rid)) ) error("The CHROM is not defined: \"%s\"\n", bcf_hdr_int2id(hdr,BCF_DT_CTG,rec->rid)); for (i=0; id.n_flt; i++) { int id = rec->d.flt[i]; if ( id >= hdr_out->n[BCF_DT_ID] ) break; if ( !bcf_hdr_idinfo_exists(hdr_out,BCF_HL_FLT,id) ) break; if ( strcmp(hdr->id[BCF_DT_ID][id].key,hdr_out->id[BCF_DT_ID][id].key) ) error("FIXME: Broken FILTER ids: %s vs %s\n", hdr->id[BCF_DT_ID][id].key,hdr_out->id[BCF_DT_ID][id].key); } if ( i!=rec->d.n_flt ) error("The FILTER is not defined: \"%s\"\n", bcf_hdr_int2id(hdr,BCF_DT_ID,rec->d.flt[i])); for (i=0; in_info; i++) { int id = rec->d.info[i].key; if ( id >= hdr_out->n[BCF_DT_ID] ) break; if ( !hdr_out->id[BCF_DT_ID][id].key ) break; if ( !bcf_hdr_idinfo_exists(hdr_out,BCF_HL_INFO,id) ) break; if ( strcmp(hdr->id[BCF_DT_ID][id].key,hdr_out->id[BCF_DT_ID][id].key) ) error("FIXME: Broken INFO ids: %s vs %s\n", hdr->id[BCF_DT_ID][id].key,hdr_out->id[BCF_DT_ID][id].key); } if ( i!=rec->n_info ) error("The INFO tag is not defined: \"%s\"\n", bcf_hdr_int2id(hdr,BCF_DT_ID,rec->d.info[i].key)); for (i=0; in_fmt; i++) { int id = rec->d.fmt[i].id; if ( id >= hdr_out->n[BCF_DT_ID] ) break; if ( !hdr_out->id[BCF_DT_ID][id].key ) break; if ( !bcf_hdr_idinfo_exists(hdr_out,BCF_HL_FMT,id) ) break; if ( strcmp(hdr->id[BCF_DT_ID][id].key,hdr_out->id[BCF_DT_ID][id].key) ) error("FIXME: Broken FORMAT ids: %s vs %s\n", hdr->id[BCF_DT_ID][id].key,hdr_out->id[BCF_DT_ID][id].key); } if ( i!=rec->n_fmt ) error("The FORMAT tag is not defined: \"%s\"\n", bcf_hdr_int2id(hdr,BCF_DT_ID,rec->d.fmt[i].id)); bcf_write(fp_out,hdr_out,rec); } bcf_destroy(rec); free(htxt.s); hts_close(fp_out); hts_close(fp); bcf_hdr_destroy(hdr_out); bcf_hdr_destroy(hdr); } static void usage(args_t *args) { fprintf(stderr, "\n"); fprintf(stderr, "About: Modify header of VCF/BCF files, change sample names.\n"); fprintf(stderr, "Usage: bcftools reheader [OPTIONS] \n"); fprintf(stderr, "\n"); fprintf(stderr, "Options:\n"); fprintf(stderr, " -h, --header new header\n"); fprintf(stderr, " -o, --output write output to a file [standard output]\n"); fprintf(stderr, " -s, --samples new sample names\n"); fprintf(stderr, "\n"); exit(1); } int main_reheader(int argc, char *argv[]) { int c; args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->argc = argc; args->argv = argv; static struct option loptions[] = { {"output",1,0,'o'}, {"header",1,0,'h'}, {"samples",1,0,'s'}, {0,0,0,0} }; while ((c = getopt_long(argc, argv, "s:h:o:",loptions,NULL)) >= 0) { switch (c) { case 'o': args->output_fname = optarg; break; case 's': args->samples_fname = optarg; break; case 'h': args->header_fname = optarg; break; case '?': usage(args); default: error("Unknown argument: %s\n", optarg); } } if ( optind>=argc ) { if ( !isatty(fileno((FILE *)stdin)) ) args->fname = "-"; // reading from stdin else usage(args); } else args->fname = argv[optind]; if ( !args->samples_fname && !args->header_fname ) usage(args); if ( !args->fname ) usage(args); args->fp = hts_open(args->fname,"r"); if ( !args->fp ) error("Failed to open: %s\n", args->fname); args->type = *hts_get_format(args->fp); if ( args->type.format==vcf ) { if ( args->type.compression==bgzf || args->type.compression==gzip ) reheader_vcf_gz(args); else reheader_vcf(args); } else reheader_bcf(args, args->type.compression==bgzf || args->type.compression==gzip); free(args); return 0; } bcftools-1.2/tabix.c000066400000000000000000000131641246371514100144530ustar00rootroot00000000000000/* tabix.c -- tabix subcommand. Copyright (C) 2012 Broad Institute. Copyright (C) 2013 Genome Research Ltd. Author: Heng Li Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include int main_tabix(int argc, char *argv[]) { int c, min_shift = -1, is_force = 0, is_all = 0; tbx_conf_t conf = tbx_conf_gff, *conf_ptr = NULL; while ((c = getopt(argc, argv, "0fap:s:b:e:S:c:m:")) >= 0) if (c == '0') conf.preset |= TBX_UCSC; else if (c == 'f') is_force = 1; else if (c == 'a') is_all = 1; else if (c == 'm') min_shift = atoi(optarg); else if (c == 's') conf.sc = atoi(optarg); else if (c == 'b') conf.bc = atoi(optarg); else if (c == 'e') conf.ec = atoi(optarg); else if (c == 'c') conf.meta_char = *optarg; else if (c == 'S') conf.line_skip = atoi(optarg); else if (c == 'p') { if (strcmp(optarg, "gff") == 0) conf_ptr = &tbx_conf_gff; else if (strcmp(optarg, "bed") == 0) conf_ptr = &tbx_conf_bed; else if (strcmp(optarg, "sam") == 0) conf_ptr = &tbx_conf_sam; else if (strcmp(optarg, "vcf") == 0) conf_ptr = &tbx_conf_vcf; else { fprintf(stderr, "The type '%s' not recognised\n", optarg); return 1; } } if (optind == argc) { fprintf(stderr, "\nUsage: bcftools tabix [options] [reg1 [...]]\n\n"); fprintf(stderr, "Options: -p STR preset: gff, bed, sam or vcf [gff]\n"); fprintf(stderr, " -s INT column number for sequence names (suppressed by -p) [1]\n"); fprintf(stderr, " -b INT column number for region start [4]\n"); fprintf(stderr, " -e INT column number for region end (if no end, set INT to -b) [5]\n"); fprintf(stderr, " -0 specify coordinates are zero-based\n"); fprintf(stderr, " -S INT skip first INT lines [0]\n"); fprintf(stderr, " -c CHAR skip lines starting with CHAR [null]\n"); fprintf(stderr, " -a print all records\n"); fprintf(stderr, " -f force to overwrite existing index\n"); fprintf(stderr, " -m INT set the minimal interval size to 1<= 0) puts(s.s); bgzf_close(fp); free(s.s); } else if (optind + 2 > argc) { // create index if ( !conf_ptr ) { // auto-detect file type by file name int l = strlen(argv[optind]); int strcasecmp(const char *s1, const char *s2); if (l>=7 && strcasecmp(argv[optind]+l-7, ".gff.gz") == 0) conf_ptr = &tbx_conf_gff; else if (l>=7 && strcasecmp(argv[optind]+l-7, ".bed.gz") == 0) conf_ptr = &tbx_conf_bed; else if (l>=7 && strcasecmp(argv[optind]+l-7, ".sam.gz") == 0) conf_ptr = &tbx_conf_sam; else if (l>=7 && strcasecmp(argv[optind]+l-7, ".vcf.gz") == 0) conf_ptr = &tbx_conf_vcf; } if ( conf_ptr ) conf = *conf_ptr; if (!is_force) { char *fn; FILE *fp; fn = (char*)alloca(strlen(argv[optind]) + 5); strcat(strcpy(fn, argv[optind]), min_shift <= 0? ".tbi" : ".csi"); if ((fp = fopen(fn, "rb")) != 0) { fclose(fp); fprintf(stderr, "[E::%s] the index file exists; use option '-f' to overwrite\n", __func__); return 1; } } if ( tbx_index_build(argv[optind], min_shift, &conf) ) { fprintf(stderr,"tbx_index_build failed: Is the file bgzip-compressed? Was wrong -p [type] option used?\n"); return 1; } } else { // read with random access tbx_t *tbx; BGZF *fp; kstring_t s; int i; if ((tbx = tbx_index_load(argv[optind])) == 0) return 1; if ((fp = bgzf_open(argv[optind], "r")) == 0) return 1; s.s = 0; s.l = s.m = 0; for (i = optind + 1; i < argc; ++i) { hts_itr_t *itr; if ((itr = tbx_itr_querys(tbx, argv[i])) == 0) continue; while (tbx_bgzf_itr_next(fp, tbx, itr, &s) >= 0) puts(s.s); tbx_itr_destroy(itr); } free(s.s); bgzf_close(fp); tbx_destroy(tbx); } return 0; } bcftools-1.2/test/000077500000000000000000000000001246371514100141525ustar00rootroot00000000000000bcftools-1.2/test/23andme.fa000066400000000000000000000004371246371514100157170ustar00rootroot00000000000000>1 CACGTNACGGCTGAAGTCCAAGGTAC CGTATCGAGTTCACAGTCGATAGCTC GATCGATAGCATCGCTAGCNNNACTA CGATCGATCGCTCTCCGTAACACTCA AAAACGATCGATCGACTGCTCTTTAG CGATGACTTTAGGGGAAAAA >2 CGCTCAGCCGTACAGCCGAGCAGGAC ACGCTATTTTAGATCGACTGGCTNNG CGCTAGCTACGCTTTAGCACGAGAA >Y NNNGCATACGTGTCCATCACGATGAT AGCGATGATCGATC bcftools-1.2/test/23andme.fa.fai000066400000000000000000000000541246371514100164500ustar00rootroot000000000000001 150 3 26 27 2 77 162 26 27 Y 40 245 26 27 bcftools-1.2/test/annotate.hdr000066400000000000000000000004031246371514100164570ustar00rootroot00000000000000##INFO= ##INFO= ##INFO= ##INFO= bcftools-1.2/test/annotate.out000066400000000000000000000062361246371514100165230ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##test= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3000150 snp_3000150 C T 999 PASS AN=4;AC=2;T_INT=1,2;T_FLOAT=1e-10,2e-10 GT:GQ 0/1:245 0/1:245 1 3000151 snp_3000151 C T 999 PASS AN=4;AC=2;T_INT=1;T_FLOAT=2e-10 GT:DP:GQ 0/1:32:245 0/1:32:245 1 3062915 indel_3062915 GTTT G 12.9 q10 DP4=1,2,3,4;AN=4;AC=2;INDEL;STR=test GT:GQ:DP:GL 0/1:409:35:-20,-5,-20 0/1:409:35:-20,-5,-20 1 3062915 snp_3062915 G T,C 999 test TEST=5;DP4=1,2,3,4;AN=3;AC=1,1;T_INT=1;T_FLOAT=2e-10 GT:TT:GQ:DP:GL 0/1:0,1:409:35:-20,-5,-20,-20,-5,-20 2:0,1:409:35:-20,-5,-20 1 3106154 indel_3106154 CAAA C 342 PASS AN=4;AC=2;INDEL GT:GQ:DP 0/1:245:32 0/1:245:32 1 3106154 indel_3106154 C CT 59.2 PASS AN=4;AC=2;INDEL GT:GQ:DP 0/1:245:32 0/1:245:32 1 3157410 indel_3157410 GA G 90.6 q10 AN=4;AC=4;INDEL GT:GQ:DP 1/1:21:21 1/1:21:21 1 3162006 indel_3162006 GAA G 60.2 PASS AN=4;AC=2;INDEL GT:GQ:DP 0/1:212:22 0/1:212:22 1 3177144 snp_3177144 G T 999 PASS AN=4;AC=2;T_INT=1;T_FLOAT=2e-10 GT:GQ:DP 0/0:150:30 1/1:150:30 1 3177144 ref_3177144 G . 999 PASS AN=4;AC=0;T_INT=1;T_FLOAT=2e-10 GT:GQ:DP 0/0:150:30 0/0:150:30 1 3184885 indel_3184885 TAAAA TA,T 61.5 PASS AN=4;AC=2,2;INDEL GT:GQ:DP 1/2:12:10 1/2:12:10 2 3199812 indel_3199812 G GTT,GT 82.7 PASS AN=4;AC=2,2;INDEL GT:GQ:DP 1/2:322:26 1/2:322:26 3 3212016 indel_3212016 CTT C,CT 79 PASS AN=4;AC=2,2;INDEL GT:GQ:DP 1/2:91:26 1/2:91:26 4 3258448 indel_3258448 TACACACAC T 59.9 PASS AN=4;AC=2;INDEL GT:GQ:DP 0/1:325:31 0/1:325:31 4 4000000 id1 T A,C 59.9 PASS AN=4;AC=2;INDEL GT:GQ:DP 0/1:325:31 0/1:325:31 4 4000001 id2 T A 59.9 PASS AN=4;AC=2;INDEL GT:GQ:DP 0/1:325:31 0/1:325:31 bcftools-1.2/test/annotate.tab000066400000000000000000000012741246371514100164570ustar00rootroot000000000000003 3212016 CTT C,CT indel_3212016 . . . 1 4 3258448 TACACACAC T indel_3258448 . . . 1 4 4000000 T C id1 . . . 1 4 4000001 T C,A id2 . . . 1 2 3199812 G GTT,GT indel_3199812 . . . 1 1 3000150 C CT indel_3000150 . . . 1 1 3000150 C T snp_3000150 999 1,2 1e-10,2e-10 . 1 3000151 C T snp_3000151 999 1 2e-10 . 1 3062915 G T,C snp_3062915 999 1 2e-10 . 1 3062915 GTTT G indel_3062915 . . . 1 1 3106154 A C snp_3106154 999 1 2e-10 . 1 3106154 C CT indel_3106154 . . . 1 1 3106154 CAAA C indel_3106154 . . . 1 1 3157410 GA G indel_3157410 . . . 1 1 3162006 GAA G indel_3162006 . . . 1 1 3177144 G . ref_3177144 999 1 2e-10 . 1 3177144 G T snp_3177144 999 1 2e-10 0 1 3184885 TAAAA TA,T indel_3184885 . . . 1 bcftools-1.2/test/annotate.vcf000066400000000000000000000051311246371514100164630ustar00rootroot00000000000000##fileformat=VCFv4.1 ##INFO= ##FORMAT= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##test= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3000150 . C T 59.2 PASS AN=4;AC=2 GT:GQ 0/1:245 0/1:245 1 3000151 . C T 59.2 PASS AN=4;AC=2 GT:DP:GQ 0/1:32:245 0/1:32:245 1 3062915 id3D GTTT G 12.9 q10 DP4=1,2,3,4;AN=4;AC=2;INDEL;STR=test GT:GQ:DP:GL 0/1:409:35:-20,-5,-20 0/1:409:35:-20,-5,-20 1 3062915 idSNP G T,C 12.6 test TEST=5;DP4=1,2,3,4;AN=3;AC=1,1 GT:TT:GQ:DP:GL 0/1:0,1:409:35:-20,-5,-20,-20,-5,-20 2:0,1:409:35:-20,-5,-20 1 3106154 . CAAA C 342 PASS AN=4;AC=2 GT:GQ:DP 0/1:245:32 0/1:245:32 1 3106154 . C CT 59.2 PASS AN=4;AC=2 GT:GQ:DP 0/1:245:32 0/1:245:32 1 3157410 . GA G 90.6 q10 AN=4;AC=4 GT:GQ:DP 1/1:21:21 1/1:21:21 1 3162006 . GAA G 60.2 PASS AN=4;AC=2 GT:GQ:DP 0/1:212:22 0/1:212:22 1 3177144 . G T 45 PASS AN=4;AC=2 GT:GQ:DP 0/0:150:30 1/1:150:30 1 3177144 . G . 45 PASS AN=4;AC=0 GT:GQ:DP 0/0:150:30 0/0:150:30 1 3184885 . TAAAA TA,T 61.5 PASS AN=4;AC=2,2 GT:GQ:DP 1/2:12:10 1/2:12:10 2 3199812 . G GTT,GT 82.7 PASS AN=4;AC=2,2 GT:GQ:DP 1/2:322:26 1/2:322:26 3 3212016 . CTT C,CT 79 PASS AN=4;AC=2,2 GT:GQ:DP 1/2:91:26 1/2:91:26 4 3258448 . TACACACAC T 59.9 PASS AN=4;AC=2 GT:GQ:DP 0/1:325:31 0/1:325:31 4 4000000 . T A,C 59.9 PASS AN=4;AC=2 GT:GQ:DP 0/1:325:31 0/1:325:31 4 4000001 . T A 59.9 PASS AN=4;AC=2 GT:GQ:DP 0/1:325:31 0/1:325:31 bcftools-1.2/test/annotate2.out000066400000000000000000000055571246371514100166120ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##test= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3000150 . C T 59.2 PASS AN=4;AC=2;T_STR=region_3000150_3106154 GT:GQ 0/1:245 0/1:245 1 3000151 . C T 59.2 PASS AN=4;AC=2 GT:DP:GQ 0/1:32:245 0/1:32:245 1 3062915 id3D GTTT G 12.9 q10 DP4=1,2,3,4;AN=4;AC=2;INDEL;STR=test GT:GQ:DP:GL 0/1:409:35:-20,-5,-20 0/1:409:35:-20,-5,-20 1 3062915 idSNP G T,C 12.6 test TEST=5;DP4=1,2,3,4;AN=3;AC=1,1 GT:TT:GQ:DP:GL 0/1:0,1:409:35:-20,-5,-20,-20,-5,-20 2:0,1:409:35:-20,-5,-20 1 3106154 . CAAA C 342 PASS AN=4;AC=2 GT:GQ:DP 0/1:245:32 0/1:245:32 1 3106154 . C CT 59.2 PASS AN=4;AC=2 GT:GQ:DP 0/1:245:32 0/1:245:32 1 3157410 . GA G 90.6 q10 AN=4;AC=4 GT:GQ:DP 1/1:21:21 1/1:21:21 1 3162006 . GAA G 60.2 PASS AN=4;AC=2 GT:GQ:DP 0/1:212:22 0/1:212:22 1 3177144 . G T 45 PASS AN=4;AC=2 GT:GQ:DP 0/0:150:30 1/1:150:30 1 3177144 . G . 45 PASS AN=4;AC=0 GT:GQ:DP 0/0:150:30 0/0:150:30 1 3184885 . TAAAA TA,T 61.5 PASS AN=4;AC=2,2 GT:GQ:DP 1/2:12:10 1/2:12:10 2 3199812 . G GTT,GT 82.7 PASS AN=4;AC=2,2 GT:GQ:DP 1/2:322:26 1/2:322:26 3 3212016 . CTT C,CT 79 PASS AN=4;AC=2,2 GT:GQ:DP 1/2:91:26 1/2:91:26 4 3258448 . TACACACAC T 59.9 PASS AN=4;AC=2 GT:GQ:DP 0/1:325:31 0/1:325:31 4 4000000 . T A,C 59.9 PASS AN=4;AC=2 GT:GQ:DP 0/1:325:31 0/1:325:31 4 4000001 . T A 59.9 PASS AN=4;AC=2 GT:GQ:DP 0/1:325:31 0/1:325:31 bcftools-1.2/test/annotate2.tab000066400000000000000000000001221246371514100165300ustar00rootroot000000000000002 3000000 3199812 region_3000000_3199812 1 3000150 3106154 region_3000150_3106154 bcftools-1.2/test/annotate2.vcf000066400000000000000000000021331246371514100165440ustar00rootroot00000000000000##fileformat=VCFv4.1 ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B C 1 3000001 xx C T 11 PASS FLAG;IINT=11;IFLT=1.1;ISTR=xxx GT:FINT:FFLT:FSTR 0/0:11:1.1:xxx 0/0:11:1.1:x 0/0:11:1.1:x 1 3000002 . C T . . . GT . . . 1 3000003 xx C T 11 q11 FLAG;IINT=.;IFLT=.;ISTR=. GT:FINT:FFLT:FSTR 0/0:.:.:. 0/0:.:.:. 0/0:.:.:. 1 3000004 xx C T 11 q11 FLAG;IINT=11;IFLT=1.1;ISTR=xxx GT:FINT:FFLT:FSTR 0/0:11:1.1:x 0/0:11:1.1:xxx 0/0:11:1.1:xxx bcftools-1.2/test/annotate3.out000066400000000000000000000054061246371514100166040ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##test= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3000150 id1 C T 99 PASS AN=4;AC=2;STR=id1 GT:GQ 0/1:245 0/1:245 1 3000151 id2 C T 99 PASS AN=4;AC=2;STR=id2 GT:DP:GQ 0/1:32:245 0/1:32:245 1 3062915 idIndel GTTT G 99 PASS DP4=1,2,3,4;AN=4;AC=2;INDEL;STR=testIndel GT:GQ:DP:GL 0/1:409:35:-20,-5,-20 0/1:409:35:-20,-5,-20 1 3062915 idSNP G T,C 99 PASS TEST=5;DP4=1,2,3,4;AN=3;AC=1,1;STR=testSNP GT:TT:GQ:DP:GL 0/1:0,1:409:35:-20,-5,-20,-20,-5,-20 2:0,1:409:35:-20,-5,-20 1 3106154 id4 CAAA C 99 PASS AN=4;AC=2;STR=id4 GT:GQ:DP 0/1:245:32 0/1:245:32 1 3106154 id5 C CT 99 PASS AN=4;AC=2;STR=id5 GT:GQ:DP 0/1:245:32 0/1:245:32 1 3157410 id6 GA G 99 PASS AN=4;AC=4;STR=id6 GT:GQ:DP 1/1:21:21 1/1:21:21 1 3162006 . GAA G 60.2 PASS AN=4;AC=2 GT:GQ:DP 0/1:212:22 0/1:212:22 1 3177144 id8 G T 99 PASS AN=4;AC=2;STR=id8 GT:GQ:DP 0/0:150:30 1/1:150:30 1 3177144 id9 G . 99 PASS AN=4;AC=0;STR=id9 GT:GQ:DP 0/0:150:30 0/0:150:30 1 3184885 id10 TAAAA TA,T 99 PASS AN=4;AC=2,2;STR=id10 GT:GQ:DP 1/2:12:10 1/2:12:10 2 3199812 id11 G GTT,GT 99 PASS AN=4;AC=2,2;STR=id11 GT:GQ:DP 1/2:322:26 1/2:322:26 3 3212016 id12 CTT C,CT 99 PASS AN=4;AC=2,2;STR=id12 GT:GQ:DP 1/2:91:26 1/2:91:26 4 3258448 id13 TACACACAC T 99 PASS AN=4;AC=2;STR=id13 GT:GQ:DP 0/1:325:31 0/1:325:31 4 4000000 . T A,C 59.9 PASS AN=4;AC=2 GT:GQ:DP 0/1:325:31 0/1:325:31 4 4000001 . T A 59.9 PASS AN=4;AC=2 GT:GQ:DP 0/1:325:31 0/1:325:31 bcftools-1.2/test/annotate3.vcf000066400000000000000000000022351246371514100165500ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##INFO= ##INFO= ##INFO= ##INFO= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3000000 id C . 20 . AA=1;BB=2;X=3;Y=4 GT:X:PL:Y:AA 0/1:1:2:3:1 0/1:1:2:3:1 1 3000001 id C . 20 PASS AA=1;BB=2;X=3;Y=4 GT:X:PL:Y:AA 0/1:1:2:3:1 0/1:1:2:3:1 1 3000002 id C . 20 fltY;fltA;fltB;fltX BB=2;X=3;Y=4;AA=1 GT:Y:X:PL:AA 0/1:3:1:2:1 0/1:3:1:2:1 bcftools-1.2/test/annotate4.hdr000066400000000000000000000001541246371514100165460ustar00rootroot00000000000000##INFO= ##INFO= bcftools-1.2/test/annotate4.out000066400000000000000000000025241246371514100166030ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B C 1 3000001 xx C T 11 . IINT=11;IFLT=1.1;ISTR=xxx GT:FINT:FFLT:FSTR .:11:1.1:xxx .:11:1.1:x 0/0:11:1.1:x 1 3000002 id C T 99 q99 FLAG;IINT=88,99;IFLT=8.8,9.9;ISTR=888,999 GT:FINT:FFLT:FSTR 0|1:77:7.7:77 1|1:88,99:8.8,9.9:888,999 .:.:.:. 1 3000003 id C T 99 q99 FLAG;IINT=88,99;IFLT=8.8,9.9;ISTR=888,999 GT:FINT:FFLT:FSTR 0|1:77:7.7:77 1|1:88,99:8.8,9.9:888,999 0/0:.:.:. 1 3000004 id C T 99 q99 FLAG;IINT=88,99;IFLT=8.8,9.9;ISTR=888,999 GT:FINT:FFLT:FSTR 0|1:77:7.7:77 1|1:88,99:8.8,9.9:888,999 0/0:11:1.1:xxx bcftools-1.2/test/annotate4.vcf000066400000000000000000000011661246371514100165530ustar00rootroot00000000000000##fileformat=VCFv4.2 ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta #CHROM POS ID REF ALT QUAL FILTER INFO 1 1 . C T . . . 1 2 . C T,G . . FA=.,9.9;FR=.,9.9,.;IA=.,99;IR=.,99,.;SA=.,99;SR=.,99,. 1 3 . C A,T . . . bcftools-1.2/test/annotate5.out000066400000000000000000000023771246371514100166120ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B C 1 3000001 xx C T 11 PASS IINT=11;IFLT=1.1;ISTR=xxx GT:FINT:FFLT:FSTR .:11:1.1:xxx 0/0:11:1.1:x 0/0:11:1.1:x 1 3000002 id C T 99 q99 FLAG;IINT=88,99;IFLT=8.8,9.9;ISTR=888,999 GT 0|1 . . 1 3000003 id C T 99 q11;q99 FLAG;IINT=88,99;IFLT=8.8,9.9;ISTR=888,999 GT:FINT:FFLT:FSTR 0|1:.:.:. 0/0:.:.:. 0/0:.:.:. 1 3000004 id C T 99 q11;q99 FLAG;IINT=11;IFLT=1.1;ISTR=xxx GT:FINT:FFLT:FSTR 0|1:11:1.1:x 0/0:11:1.1:xxx 0/0:11:1.1:xxx bcftools-1.2/test/annotate6.out000066400000000000000000000013231246371514100166010ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##FORMAT= ##FORMAT= ##INFO= ##INFO= ##FILTER= ##FILTER= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3000000 . C . . . AA=1;BB=2 GT:PL 0/1:2 0/1:2 1 3000001 . C . . . AA=1;BB=2 GT:PL 0/1:2 0/1:2 1 3000002 . C . . fltA;fltB BB=2;AA=1 GT:PL 0/1:2 0/1:2 bcftools-1.2/test/annotate7.out000066400000000000000000000015421246371514100166050ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##FORMAT= ##INFO= ##INFO= ##INFO= ##INFO= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3000000 id C . 20 . AA=1;BB=2;X=3;Y=4 GT 0/1 0/1 1 3000001 id C . 20 PASS AA=1;BB=2;X=3;Y=4 GT 0/1 0/1 1 3000002 id C . 20 fltY;fltA;fltB;fltX BB=2;X=3;Y=4;AA=1 GT 0/1 0/1 bcftools-1.2/test/annotate8.out000066400000000000000000000014221246371514100166030ustar00rootroot00000000000000##fileformat=VCFv4.2 ##FILTER= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta #CHROM POS ID REF ALT QUAL FILTER INFO 1 1 . C T . . FA=1.1;FR=1.1,2.2;IA=1;IR=1,2;SA=11;SR=1,222 1 2 . C T,G . . FA=1.1,9.9;FR=1.1,9.9,3.3;IA=1,2;IR=1,2,3;SA=11,99;SR=111,99,3 1 3 . C A,T . . FA=.,1.1;FR=1.1,.,2.2;IA=.,1;IR=1,.,2;SA=.,11;SR=11,.,2 bcftools-1.2/test/annots.vcf000066400000000000000000000051261246371514100161600ustar00rootroot00000000000000##fileformat=VCFv4.1 ##INFO= ##FORMAT= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##test= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3000150 id1 C T 99 PASS STR=id1;AN=4;AC=0 GT:GQ 0|0:999 0|0:999 1 3000151 id2 C T 99 PASS STR=id2;AN=4;AC=0 GT:DP:GQ 0|0:99:999 0|0:99:999 1 3062915 idIndel GTTT G 99 PASS DP4=1,2,3,4;AN=4;AC=0;INDEL;STR=testIndel GT:GQ:DP:GL 0|0:999:99:-99,-9,-99 0|0:999:99:-99,-9,-99 1 3062915 idSNP G T,C 99 PASS STR=testSNP;TEST=5;DP4=1,2,3,4;AN=3;AC=0,0 GT:TT:GQ:DP:GL 0|0:9,9:999:99:-99,-9,-99,-99,-9,-99 0:9,9:999:99:-99,-9,-99 1 3106154 id4 CAAA C 99 PASS STR=id4;AN=4;AC=0 GT:GQ:DP 0|0:999:99 0|0:999:99 1 3106154 id5 C CT 99 PASS STR=id5;AN=4;AC=0 GT:GQ:DP 0|0:999:99 0|0:999:99 1 3157410 id6 GA GC,G 99 PASS STR=id6;AN=4;AC=0 GT:GQ:DP 0|0:99:99 0|0:99:99 1 3162006 id7 GAA GG 99 PASS STR=id7;AN=4;AC=0 GT:GQ:DP 0|0:999:99 0|0:999:99 1 3177144 id8 G T 99 PASS STR=id8;AN=4;AC=0 GT:GQ:DP 0|0:999:99 0|0:999:99 1 3177144 id9 G . 99 PASS STR=id9;AN=4;AC=0 GT:GQ:DP 0|0:999:99 0|0:999:99 1 3184885 id10 TAAAA TA,T 99 PASS STR=id10;AN=4;AC=0,0 GT:GQ:DP 0|0:99:99 0|0:99:99 2 3199812 id11 G GTT,GT 99 PASS STR=id11;AN=4;AC=0,0 GT:GQ:DP 0|0:999:99 0|0:999:99 3 3212016 id12 CTT C,CT 99 PASS STR=id12;AN=4;AC=0,0 GT:GQ:DP 0|0:99:99 0|0:99:99 4 3258448 id13 TACACACAC T 99 PASS STR=id13;AN=4;AC=0 GT:GQ:DP 0|0:999:99 0|0:999:99 bcftools-1.2/test/annots2.vcf000066400000000000000000000022021246371514100162320ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT B A 1 3000001 . C T . . . GT . . 1 3000002 id C T 99 q99 FLAG;IINT=88,99;IFLT=8.8,9.9;ISTR=888,999 GT:FINT:FFLT:FSTR 1|1:88,99:8.8,9.9:888,999 0|1:77:7.7:77 1 3000003 id C T 99 q99 FLAG;IINT=88,99;IFLT=8.8,9.9;ISTR=888,999 GT:FINT:FFLT:FSTR 1|1:88,99:8.8,9.9:888,999 0|1:77:7.7:77 1 3000004 id C T 99 q99 FLAG;IINT=88,99;IFLT=8.8,9.9;ISTR=888,999 GT:FINT:FFLT:FSTR 1|1:88,99:8.8,9.9:888,999 0|1:77:7.7:77 bcftools-1.2/test/annots4.tab000066400000000000000000000002341246371514100162270ustar00rootroot000000000000001 1 C A,T,G 0,1.1,0 1.1,0,2.2,0 0,1,0 1,0,2,0 X,11,XXX 1,XX,222,XXX 1 2 C T,G 1.1,2.2 1.1,2.2,3.3 1,2 1,2,3 11,2 111,22,3 1 3 C T 1.1 1.1,2.2 1 1,2 11 11,2 bcftools-1.2/test/annots4.vcf000066400000000000000000000013601246371514100162400ustar00rootroot00000000000000##fileformat=VCFv4.2 ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta #CHROM POS ID REF ALT QUAL FILTER INFO 1 1 . C A,T,G . . FA=0,1.1,0;FR=1.1,0,2.2,0;IA=0,1,0;IR=1,0,2,0;SA=X,11,XXX;SR=1,XX,222,XXX 1 2 . C T,G . . FA=1.1,2.2;FR=1.1,2.2,3.3;IA=1,2;IR=1,2,3;SA=11,2;SR=111,22,3 1 3 . C T . . FA=1.1;FR=1.1,2.2;IA=1;IR=1,2;SA=11;SR=11,2 bcftools-1.2/test/check.chk000066400000000000000000000051111246371514100157140ustar00rootroot00000000000000# # Definition of sets: # ID [2]id [3]tab-separated file names # SN, Summary numbers: # SN [2]id [3]key [4]value SN 0 number of samples: 2 SN 0 number of records: 18 SN 0 number of SNPs: 5 SN 0 number of MNPs: 1 SN 0 number of indels: 9 SN 0 number of others: 2 SN 0 number of multiallelic sites: 6 SN 0 number of multiallelic SNP sites: 1 # TSTV, transitions/transversions: # TSTV [2]id [3]ts [4]tv [5]ts/tv [6]ts (1st ALT) [7]tv (1st ALT) [8]ts/tv (1st ALT) TSTV 0 3 2 1.50 3 1 3.00 # SiS, Singleton stats: # SiS [2]id [3]allele count [4]number of SNPs [5]number of transitions [6]number of transversions [7]number of indels [8]repeat-consistent [9]repeat-inconsistent [10]not applicable SiS 0 1 3 1 2 0 0 0 0 # AF, Stats by non-reference allele frequency: # AF [2]id [3]allele frequency [4]number of SNPs [5]number of transitions [6]number of transversions [7]number of indels [8]repeat-consistent [9]repeat-inconsistent [10]not applicable AF 0 0.000000 3 1 2 2 0 0 2 AF 0 49.000000 0 0 0 12 0 0 12 AF 0 74.000000 1 1 0 0 0 0 0 AF 0 99.000000 1 1 0 0 0 0 0 # QUAL, Stats by quality: # QUAL [2]id [3]Quality [4]number of SNPs [5]number of transitions (1st ALT) [6]number of transversions (1st ALT) [7]number of indels QUAL 0 12 1 0 1 1 QUAL 0 45 0 0 0 1 QUAL 0 59 2 1 0 3 QUAL 0 60 1 1 0 0 QUAL 0 61 0 0 0 1 QUAL 0 79 0 0 0 1 QUAL 0 82 0 0 0 1 QUAL 0 90 1 1 0 0 QUAL 0 342 0 0 0 1 # IDD, InDel distribution: # IDD [2]id [3]length (deletions negative) [4]count IDD 0 -10 1 IDD 0 -8 1 IDD 0 -4 3 IDD 0 -3 4 IDD 0 -2 1 IDD 0 -1 2 IDD 0 1 1 IDD 0 2 1 # ST, Substitution types: # ST [2]id [3]type [4]count ST 0 A>C 0 ST 0 A>G 0 ST 0 A>T 0 ST 0 C>A 0 ST 0 C>G 0 ST 0 C>T 0 ST 0 G>A 3 ST 0 G>C 1 ST 0 G>T 1 ST 0 T>A 0 ST 0 T>C 0 ST 0 T>G 0 # DP, Depth distribution # DP [2]id [3]bin [4]number of genotypes [5]fraction of genotypes (%) [6]number of sites [7]fraction of sites (%) DP 0 10 2 5.555556 0 0.000000 DP 0 21 2 5.555556 0 0.000000 DP 0 22 2 5.555556 0 0.000000 DP 0 26 4 11.111111 0 0.000000 DP 0 30 2 5.555556 0 0.000000 DP 0 31 16 44.444444 0 0.000000 DP 0 32 4 11.111111 0 0.000000 DP 0 35 4 11.111111 0 0.000000 # PSC, Per-sample counts # PSC [2]id [3]sample [4]nRefHom [5]nNonRefHom [6]nHets [7]nTransitions [8]nTransversions [9]nIndels [10]average depth [11]nSingletons PSC 0 A 0 2 3 3 2 9 28.7 1 PSC 0 B 1 1 3 2 2 9 28.7 0 # HWE # HWE [2]id [3]1st ALT allele frequency [4]Number of observations [5]25th percentile [6]median [7]75th percentile HWE 0 0.000000 2 0.490000 0.490000 0.990000 HWE 0 49.000000 14 0.990000 0.990000 0.990000 HWE 0 74.000000 1 0.490000 0.490000 0.490000 HWE 0 99.000000 1 0.000000 0.000000 0.000000 bcftools-1.2/test/check.gs.chrom.gen000066400000000000000000000007241246371514100174440ustar00rootroot000000000000001 1:3062915_GTTT_G 3062915 GTTT G 0 1 0 0 1 0 1 1:3106154_CAAA_C 3106154 CAAA C 0 1 0 0 1 0 1 1:3157410_G_A 3157410 G A 0 0 1 0 0 1 1 1:3162006_G_A 3162006 G A 0 0 1 0 1 0 1 1:3177144_GT_G 3177144 GT G 0 1 0 0 1 0 4 4:3258448_TACACACAC_T 3258448 TACACACAC T 0 1 0 0 1 0 4 4:3258451_AAA_AGT 3258451 AAA AGT 0 1 0 0 1 0 4 4:3258452_AAA_AGA 3258452 AAA AGA 0 1 0 0 1 0 4 4:3258453_AACA_AGA 3258453 AACA AGA 0 1 0 0 1 0 4 4:3258454_AACA_AACA 3258454 AACA AACA 0 1 0 0 1 0 bcftools-1.2/test/check.gs.chrom.samples000066400000000000000000000000441246371514100203320ustar00rootroot00000000000000ID_1 ID_2 missing 0 0 0 A A 0 B B 0 bcftools-1.2/test/check.gs.vcfids.gen000066400000000000000000000007271246371514100176150ustar00rootroot000000000000001:3062915_GTTT_G id3D 3062915 GTTT G 0 1 0 0 1 0 1:3106154_CAAA_C . 3106154 CAAA C 0 1 0 0 1 0 1:3157410_G_A . 3157410 G A 0 0 1 0 0 1 1:3162006_G_A . 3162006 G A 0 0 1 0 1 0 1:3177144_GT_G . 3177144 GT G 0 1 0 0 1 0 4:3258448_TACACACAC_T . 3258448 TACACACAC T 0 1 0 0 1 0 4:3258451_AAA_AGT . 3258451 AAA AGT 0 1 0 0 1 0 4:3258452_AAA_AGA . 3258452 AAA AGA 0 1 0 0 1 0 4:3258453_AACA_AGA . 3258453 AACA AGA 0 1 0 0 1 0 4:3258454_AACA_AACA . 3258454 AACA AACA 0 1 0 0 1 0 bcftools-1.2/test/check.gs.vcfids.samples000066400000000000000000000000441246371514100205000ustar00rootroot00000000000000ID_1 ID_2 missing 0 0 0 A A 0 B B 0 bcftools-1.2/test/check.gs.vcfids_chrom.gen000066400000000000000000000004751246371514100210050ustar00rootroot000000000000001 id3D 3062915 GTTT G 0 1 0 0 1 0 1 . 3106154 CAAA C 0 1 0 0 1 0 1 . 3157410 G A 0 0 1 0 0 1 1 . 3162006 G A 0 0 1 0 1 0 1 . 3177144 GT G 0 1 0 0 1 0 4 . 3258448 TACACACAC T 0 1 0 0 1 0 4 . 3258451 AAA AGT 0 1 0 0 1 0 4 . 3258452 AAA AGA 0 1 0 0 1 0 4 . 3258453 AACA AGA 0 1 0 0 1 0 4 . 3258454 AACA AACA 0 1 0 0 1 0 bcftools-1.2/test/check.gs.vcfids_chrom.samples000066400000000000000000000000441246371514100216700ustar00rootroot00000000000000ID_1 ID_2 missing 0 0 0 A A 0 B B 0 bcftools-1.2/test/check.vcf000066400000000000000000000050641246371514100157340ustar00rootroot00000000000000##fileformat=VCFv4.1 ##INFO= ##FORMAT= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3062915 id3D GTTT G 12.9 q10 DP4=1,2,3,4;AN=4;AC=2 GT:GQ:DP:GL 0/1:409:35:-20,-5,-20 0/1:409:35:-20,-5,-20 1 3062915 idSNP G T,C 12.6 test TEST=5;DP4=1,2,3,4;AN=4;AC=1,1 GT:TT:GQ:DP:GL 0/1:0,1:409:35:-20,-5,-20,-20,-5,-20 0/2:0,1:409:35:-20,-5,-20,-20,-5,-20 1 3106154 . CAAA C 342 PASS AN=4;AC=2 GT:GQ:DP 0/1:245:32 0/1:245:32 1 3106154 . G A 59.2 PASS AN=4;AC=1 GT:GQ:DP 0/1:245:32 0/0:245:32 1 3157410 . G A 90.6 q10 AN=4;AC=4 GT:GQ:DP 1/1:21:21 1/1:21:21 1 3162006 . G A 60.2 PASS AN=4;AC=3 GT:GQ:DP 1/1:212:22 0/1:212:22 1 3177144 . GT G 45 PASS AN=4;AC=2 GT:GQ:DP 0/1:150:30 0/1:150:30 1 3184885 . TAAAA TA,T 61.5 PASS AN=4;AC=2,2 GT:GQ:DP 1/2:12:10 1/2:12:10 2 3199812 . G GTT,GT 82.7 PASS AN=4;AC=2,2 GT:GQ:DP 1/2:322:26 1/2:322:26 3 3212016 . CTT C,CT 79 PASS AN=4;AC=2,2 GT:GQ:DP 1/2:91:26 1/2:91:26 4 3258448 . TACACACAC T 59.9 PASS AN=4;AC=2 GT:GQ:DP 0/1:325:31 0/1:325:31 4 3258449 . GCAAA GA,G 59.9 PASS AN=4;AC=2 GT:GQ:DP 0/1:325:31 0/1:325:31 4 3258450 . AAAAGAAAAAG A,AAAAAAG 59.9 PASS AN=4;AC=2 GT:GQ:DP 0/1:325:31 0/1:325:31 4 3258451 . AAA AGT 59.9 PASS AN=4;AC=2 GT:GQ:DP 0/1:325:31 0/1:325:31 4 3258452 . AAA AGA 59.9 PASS AN=4;AC=2 GT:GQ:DP 0/1:325:31 0/1:325:31 4 3258453 . AACA AGA 59.9 PASS AN=4;AC=2 GT:GQ:DP 0/1:325:31 0/1:325:31 4 3258453 . ACA AAGA 59.9 PASS AN=4;AC=2 GT:GQ:DP 0/1:325:31 0/1:325:31 4 3258454 . AACA AACA 59.9 PASS AN=4;AC=2 GT:GQ:DP 0/1:325:31 0/1:325:31 bcftools-1.2/test/concat.1.a.vcf000066400000000000000000000033561246371514100165060ustar00rootroot00000000000000##fileformat=VCFv4.0 ##FILTER= ##FILTER= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##contig= ##contig= ##samtoolsVersion=0.2.0-rc10+htslib-0.2.0-rc10 ##samtoolsCommand=samtools mpileup -t INFO/DPR -C50 -pm3 -F0.2 -d10000 -ug -r 1:1-1000000 -b mpileup.2014-07-03//lists/chr1-pooled.list -f human_g1k_v37.fasta ##ALT= ##bcftools_callVersion=0.2.0-rc10-2-gcd94fde+htslib-0.2.0-rc10 ##bcftools_callCommand=call -vm -f GQ -S mpileup.2014-07-03//pooled/1/1:1-1000000.samples - #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A 1 100 . GTTT G 1806 q10 XX=11;DP=35 GT:GQ:DP 0/1:409:35 1 110 . C T,G 1792 Fail DP=32 GT:GQ:DP 0/1:245:32 1 110 . CAAA C 1792 Fail DP=32 GT:GQ:DP 0/1:245:32 1 120 . GA G 628 q10 DP=21 GT:GQ:DP 1/1:21:21 1 130 . G T 1016 Fail DP=22 GT:GQ:DP 0/1:212:22 1 130 . GAA GG 1016 Fail DP=22 GT:GQ:DP 0/1:212:22 1 140 . GT G 727 PASS DP=30 GT:GQ:DP 0/1:150:30 1 150 . TAAAA TA,T 246 Fail DP=10 GT:GQ:DP 1/2:12:10 1 160 . TAAAA TA,T 246 Fail DP=10 GT:GQ:DP 1/2:12:10 2 100 . GTTT G 1806 q10 DP=35 GT:GQ:DP 0/1:409:35 2 110 . CAAA C 1792 PASS DP=32 GT:GQ:DP 0/1:245:32 2 120 . GA G 628 q10 DP=21 GT:GQ:DP 1/1:21:21 2 130 . GAA G 1016 PASS DP=22 GT:GQ:DP 0/1:212:22 2 140 . GT G 727 PASS DP=30 GT:GQ:DP 0/1:150:30 2 150 . TAAAA TA,T 246 PASS DP=10 GT:GQ:DP 1/2:12:10 2 160 . TAAAA TA,TC,T 246 PASS DP=10 GT:GQ:DP 0/2:12:10 bcftools-1.2/test/concat.1.b.vcf000066400000000000000000000021601246371514100164770ustar00rootroot00000000000000##fileformat=VCFv4.0 ##samtoolsVersion=0.2.0-rc10+htslib-0.2.0-rc10 ##samtoolsCommand=samtools mpileup -t INFO/DPR -C50 -pm3 -F0.2 -d10000 -ug -r 1:1-1000000 -b mpileup.2014-07-03//lists/chr1-pooled.list -f human_g1k_v37.fasta ##ALT= ##bcftools_callVersion=0.2.0-rc10-2-gcd94fde+htslib-0.2.0-rc10 ##bcftools_callCommand=call -vm -f GQ -S mpileup.2014-07-03//pooled/1/1:1-1000000.samples - ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A 3 142 . GTTT G 1806 q10 DP=35 GT:GQ:DP 0/1:409:35 3 152 . CAAA C 1792 PASS DP=32 GT:GQ:DP 0/1:245:32 3 162 . GA G 628 q10 DP=21 GT:GQ:DP 1/1:21:21 3 172 . GAA G 1016 PASS DP=22 GT:GQ:DP 0/1:212:22 3 182 . GT G 727 PASS DP=30 GT:GQ:DP 0/1:150:30 3 192 . TAAAA TA,T 246 PASS DP=10 GT:GQ:DP 1/2:12:10 bcftools-1.2/test/concat.1.bcf.out000066400000000000000000000037211246371514100170450ustar00rootroot00000000000000##fileformat=VCFv4.0 ##FILTER= ##FILTER= ##FILTER= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##contig= ##contig= ##samtoolsVersion=0.2.0-rc10+htslib-0.2.0-rc10 ##samtoolsCommand=samtools mpileup -t INFO/DPR -C50 -pm3 -F0.2 -d10000 -ug -r 1:1-1000000 -b mpileup.2014-07-03//lists/chr1-pooled.list -f human_g1k_v37.fasta ##ALT= ##contig= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A 1 100 . GTTT G 1806 q10 XX=11;DP=35 GT:GQ:DP 0/1:409:35 1 110 . C T,G 1792 Fail DP=32 GT:GQ:DP 0/1:245:32 1 110 . CAAA C 1792 Fail DP=32 GT:GQ:DP 0/1:245:32 1 120 . GA G 628 q10 DP=21 GT:GQ:DP 1/1:21:21 1 130 . G T 1016 Fail DP=22 GT:GQ:DP 0/1:212:22 1 130 . GAA GG 1016 Fail DP=22 GT:GQ:DP 0/1:212:22 1 140 . GT G 727 PASS DP=30 GT:GQ:DP 0/1:150:30 1 150 . TAAAA TA,T 246 Fail DP=10 GT:GQ:DP 1/2:12:10 1 160 . TAAAA TA,T 246 Fail DP=10 GT:GQ:DP 1/2:12:10 2 100 . GTTT G 1806 q10 DP=35 GT:GQ:DP 0/1:409:35 2 110 . CAAA C 1792 PASS DP=32 GT:GQ:DP 0/1:245:32 2 120 . GA G 628 q10 DP=21 GT:GQ:DP 1/1:21:21 2 130 . GAA G 1016 PASS DP=22 GT:GQ:DP 0/1:212:22 2 140 . GT G 727 PASS DP=30 GT:GQ:DP 0/1:150:30 2 150 . TAAAA TA,T 246 PASS DP=10 GT:GQ:DP 1/2:12:10 2 160 . TAAAA TA,TC,T 246 PASS DP=10 GT:GQ:DP 0/2:12:10 3 142 . GTTT G 1806 q10 DP=35 GT:GQ:DP 0/1:409:35 3 152 . CAAA C 1792 PASS DP=32 GT:GQ:DP 0/1:245:32 3 162 . GA G 628 q10 DP=21 GT:GQ:DP 1/1:21:21 3 172 . GAA G 1016 PASS DP=22 GT:GQ:DP 0/1:212:22 3 182 . GT G 727 PASS DP=30 GT:GQ:DP 0/1:150:30 3 192 . TAAAA TA,T 246 PASS DP=10 GT:GQ:DP 1/2:12:10 bcftools-1.2/test/concat.1.vcf.out000066400000000000000000000037211246371514100170710ustar00rootroot00000000000000##fileformat=VCFv4.0 ##FILTER= ##FILTER= ##FILTER= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##contig= ##contig= ##samtoolsVersion=0.2.0-rc10+htslib-0.2.0-rc10 ##samtoolsCommand=samtools mpileup -t INFO/DPR -C50 -pm3 -F0.2 -d10000 -ug -r 1:1-1000000 -b mpileup.2014-07-03//lists/chr1-pooled.list -f human_g1k_v37.fasta ##ALT= ##contig= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A 1 100 . GTTT G 1806 q10 XX=11;DP=35 GT:GQ:DP 0/1:409:35 1 110 . C T,G 1792 Fail DP=32 GT:GQ:DP 0/1:245:32 1 110 . CAAA C 1792 Fail DP=32 GT:GQ:DP 0/1:245:32 1 120 . GA G 628 q10 DP=21 GT:GQ:DP 1/1:21:21 1 130 . G T 1016 Fail DP=22 GT:GQ:DP 0/1:212:22 1 130 . GAA GG 1016 Fail DP=22 GT:GQ:DP 0/1:212:22 1 140 . GT G 727 PASS DP=30 GT:GQ:DP 0/1:150:30 1 150 . TAAAA TA,T 246 Fail DP=10 GT:GQ:DP 1/2:12:10 1 160 . 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T A 246 PASS DP=10 GT:PS 0|1:380 1|0:380 3 460 . T A 246 PASS DP=10 GT:PS 0|1:380 1|0:380 3 470 . T A 246 PASS DP=10 GT:PS 0|1:380 1|0:380 3 480 . T A 246 PASS DP=10 GT:PS 0|1:380 1|0:380 3 490 . T A 246 PASS DP=10 GT:PS 0|1:380 1|0:380 bcftools-1.2/test/concat.3.c.vcf000066400000000000000000000016561246371514100165130ustar00rootroot00000000000000##fileformat=VCFv4.0 ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 2 280 . T A 246 PASS DP=10 GT 1|0 0|1 2 290 . T A 246 PASS DP=10 GT 1|0 1|0 2 300 . T A 246 PASS DP=10 GT 0|1 1|0 2 310 . T A 246 PASS DP=10 GT 0|1 1|0 2 320 . T A 246 PASS DP=10 GT 0|1 1|0 2 330 . T A 246 PASS DP=10 GT 0|1 1|0 2 340 . T A 246 PASS DP=10 GT 0|1 1|0 2 350 . T A 246 PASS DP=10 GT 0|1 1|0 2 360 . T A 246 PASS DP=10 GT 0|1 1|0 2 370 . T A 246 PASS DP=10 GT 0|1 1|0 2 380 . T A 246 PASS DP=10 GT 0|1 1|0 2 390 . T A 246 PASS DP=10 GT 0|1 1|0 2 400 . T A 246 PASS DP=10 GT 0|1 1|0 2 410 . T A 246 PASS DP=10 GT 0|1 1|0 bcftools-1.2/test/concat.3.d.vcf000066400000000000000000000015421246371514100165060ustar00rootroot00000000000000##fileformat=VCFv4.0 ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 2 300 . T A 246 PASS DP=10 GT 1|0 1|0 2 320 . T A 246 PASS DP=10 GT 1|0 1|0 2 330 . T A 246 PASS DP=10 GT 1|0 1|0 2 340 . T A 246 PASS DP=10 GT 1|0 1|0 2 350 . T A 246 PASS DP=10 GT 1|0 1|0 2 360 . T A 246 PASS DP=10 GT 1|0 1|0 2 370 . T A 246 PASS DP=10 GT 1|0 1|0 2 380 . T A 246 PASS DP=10 GT 1|0 1|0 2 390 . T A 246 PASS DP=10 GT 1|0 1|0 2 490 . T A 246 PASS DP=10 GT 1|0 1|0 2 500 . T A 246 PASS DP=10 GT 1|0 1|0 2 510 . T A 246 PASS DP=10 GT 1|0 1|0 bcftools-1.2/test/concat.3.e.vcf000066400000000000000000000015421246371514100165070ustar00rootroot00000000000000##fileformat=VCFv4.0 ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 2 310 . T A 246 PASS DP=10 GT 0|1 0|1 2 320 . T A 246 PASS DP=10 GT 0|1 0|1 2 330 . T A 246 PASS DP=10 GT 0|1 0|1 2 340 . T A 246 PASS DP=10 GT 0|1 0|1 2 350 . T A 246 PASS DP=10 GT 0|1 0|1 2 360 . T A 246 PASS DP=10 GT 0|1 0|1 2 370 . T A 246 PASS DP=10 GT 0|1 0|1 2 380 . T A 246 PASS DP=10 GT 0|1 0|1 2 390 . T A 246 PASS DP=10 GT 0|1 0|1 2 490 . T A 246 PASS DP=10 GT 0|1 0|1 2 500 . T A 246 PASS DP=10 GT 0|1 0|1 2 510 . T A 246 PASS DP=10 GT 0|1 0|1 bcftools-1.2/test/concat.3.f.vcf000066400000000000000000000015421246371514100165100ustar00rootroot00000000000000##fileformat=VCFv4.0 ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 3 380 . T A 246 PASS DP=10 GT 1|0 1|0 3 390 . T A 246 PASS DP=10 GT 1|0 1|0 3 400 . T A 246 PASS DP=10 GT 1|0 1|0 3 410 . T A 246 PASS DP=10 GT 1|0 1|0 3 420 . T A 246 PASS DP=10 GT 1|0 1|0 3 430 . T A 246 PASS DP=10 GT 1|0 1|0 3 440 . T A 246 PASS DP=10 GT 1|0 1|0 3 450 . T A 246 PASS DP=10 GT 1|0 1|0 3 460 . T A 246 PASS DP=10 GT 1|0 1|0 3 470 . T A 246 PASS DP=10 GT 1|0 1|0 3 480 . T A 246 PASS DP=10 GT 1|0 1|0 3 490 . T A 246 PASS DP=10 GT 1|0 1|0 bcftools-1.2/test/concat.3.vcf.out000066400000000000000000000440311246371514100170720ustar00rootroot00000000000000##fileformat=VCFv4.0 ##FILTER= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= ##contig= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##contig= ##contig= ##FORMAT= ##FORMAT= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 9 202 . GTTT G 1806 q10 DP=35 GT:GQ:DP:PS 0|1:409:35:202 0|1:.:.:202 9 212 . C T,G 1792 PASS DP=32 GT:GQ:DP:PS 0|1:245:32:202 0|1:.:.:202 9 212 . CAAA C 1792 PASS DP=32 GT:GQ:DP:PS 0|1:245:32:202 0|1:.:.:202 9 222 . GA G 628 q10 DP=21 GT:GQ:DP:PS 0|1:21:21:202 0|1:.:.:202 9 232 . G T 1016 PASS DP=22 GT:GQ:DP:PS 0|1:212:22:202 0|1:.:.:202 9 232 . GAA GG 1016 PASS DP=22 GT:GQ:DP:PS 0|1:212:22:202 0|1:.:.:202 9 242 . GT G 727 PASS DP=30 GT:GQ:DP:PS 0|1:150:30:202 0|1:.:.:202 9 252 . TAAAA TA,T 246 PASS DP=10 GT:GQ:DP:PS 0|1:12:10:202 0|1:.:.:202 9 262 . TAAAA TA,T 246 PASS DP=10 GT:GQ:DP:PS 0|1:12:10:202 0|1:.:.:202 1 100 . GTTT G 1806 q10 DP=35 GT:GQ:DP:PS 0|1:409:35:100 0|1:.:.:100 1 110 . C T,G 1792 PASS DP=32 GT:GQ:DP:PS 0|1:245:32:100 0|1:.:.:100 1 110 . CAAA C 1792 PASS DP=32 GT:GQ:DP:PS 0|1:245:32:100 0|1:.:.:100 1 120 . GA G 628 q10 DP=21 GT:GQ:DP:PS 0|1:21:21:100 0|1:.:.:100 1 130 . G T 1016 PASS DP=22 GT:GQ:DP:PS 0|1:212:22:100 0|1:.:.:100 1 130 . GAA GG 1016 PASS DP=22 GT:GQ:DP:PS 0|1:212:22:100 0|1:.:.:100 1 140 . GT G 727 PASS DP=30 GT:GQ:DP:PS 0|1:150:30:100 0|1:.:.:100 1 150 . TAAAA TA,T 246 PASS DP=10 GT:GQ:DP:PS 0|1:12:10:100 0|1:.:.:100 1 160 . TAAAA TA,T 246 PASS DP=10 GT:GQ:DP:PS 0|1:12:10:100 0|1:.:.:100 2 170 . T A 246 PASS DP=10 GT:PS 1|0:170 0|1:170 2 180 . T A 246 PASS DP=10 GT:PS 1|0:170 0|1:170 2 190 . T A 246 PASS DP=10 GT:PS 1|0:170 0|1:170 2 200 . T A 246 PASS DP=10 GT:PS 1|0:170 0|1:170 2 210 . T A 246 PASS DP=10 GT:PS 1|0:170 0|1:170 2 220 . T A 246 PASS DP=10 GT:PS 1|0:170 0|1:170 2 230 . T A 246 PASS DP=10 GT:PS 1|0:170 0|1:170 2 240 . T A 246 PASS DP=10 GT:PS 1|0:170 0|1:170 2 250 . T A 246 PASS DP=10 GT:PS 1|0:170 0|1:170 2 260 . T A 246 PASS DP=10 GT:PS 1|0:170 0|1:170 2 270 . T A 246 PASS DP=10 GT:PS 1|0:170 0|1:170 2 280 . T A 246 PASS DP=10 GT:PS 1|0:170 0|1:170 2 290 . T A 246 PASS DP=10 GT:PS 1|0:170 0|1:170 2 300 . T A 246 PASS DP=10 GT:PS 1|0:170 0|1:170 2 310 . T A 246 PASS DP=10 GT:PQ:PS 1|0:3:310 0|1:28:310 2 320 . T A 246 PASS DP=10 GT:PS 1|0:310 0|1:310 2 330 . T A 246 PASS DP=10 GT:PS 1|0:310 0|1:310 2 340 . T A 246 PASS DP=10 GT:PS 1|0:310 0|1:310 2 350 . T A 246 PASS DP=10 GT:PS 1|0:310 0|1:310 2 360 . T A 246 PASS DP=10 GT:PS 1|0:310 0|1:310 2 370 . T A 246 PASS DP=10 GT:PQ:PS 1|0:99:310 0|1:99:310 2 380 . T A 246 PASS DP=10 GT:PS 1|0:310 0|1:310 2 390 . T A 246 PASS DP=10 GT:PS 1|0:310 0|1:310 2 490 . T A 246 PASS DP=10 GT:PS 1|0:310 0|1:310 2 500 . T A 246 PASS DP=10 GT:PS 1|0:310 0|1:310 2 510 . T A 246 PASS DP=10 GT:PQ:PS 1|0:99:310 0|1:99:310 3 380 . T A 246 PASS DP=10 GT:PS 0|1:380 1|0:380 3 390 . T A 246 PASS DP=10 GT:PS 0|1:380 1|0:380 3 400 . T A 246 PASS DP=10 GT:PS 0|1:380 1|0:380 3 410 . T A 246 PASS DP=10 GT:PS 0|1:380 1|0:380 3 420 . T A 246 PASS DP=10 GT:PS 0|1:380 1|0:380 3 430 . T A 246 PASS DP=10 GT:PS 0|1:380 1|0:380 3 440 . T A 246 PASS DP=10 GT:PS 0|1:380 1|0:380 3 450 . T A 246 PASS DP=10 GT:PS 0|1:380 1|0:380 3 460 . T A 246 PASS DP=10 GT:PS 0|1:380 1|0:380 3 470 . T A 246 PASS DP=10 GT:PS 0|1:380 1|0:380 3 480 . T A 246 PASS DP=10 GT:PS 0|1:380 1|0:380 3 490 . T A 246 PASS DP=10 GT:PS 0|1:380 1|0:380 bcftools-1.2/test/concat.4.bcf.out000066400000000000000000000026431246371514100170520ustar00rootroot00000000000000##fileformat=VCFv4.0 ##FILTER= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= ##contig= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A 1 100 . GTTT G 1806 q10 DP=35 GT:GQ:DP 0/1:409:35 1 110 . C T,G 1792 Fail XX=11;DP=32 GT:GQ:DP 0/1:245:32 1 110 . CAAA C 1792 PASS DP=32 GT:GQ:DP 0/1:245:32 1 120 . GA G 628 q10 DP=21 GT:GQ:DP 1/1:21:21 1 130 . GAA GG 1016 PASS DP=22 GT:GQ:DP 0/1:212:22 1 130 . G T 1016 PASS DP=22 GT:GQ:DP 0/1:212:22 1 140 . GT G 727 PASS DP=30 GT:GQ:DP 0/1:150:30 1 150 . TAAAA TA,T 246 PASS DP=10 GT:GQ:DP 1/2:12:10 1 160 . TAAAA TA,T 246 PASS DP=10 GT:GQ:DP 1/2:12:10 2 100 . GTTT G 1806 q10 DP=35 GT:GQ:DP 0/1:409:35 2 110 . CAAA C 1792 PASS DP=32 GT:GQ:DP 0/1:245:32 2 120 . GA G 628 q10 DP=21 GT:GQ:DP 1/1:21:21 2 130 . GAA G 1016 PASS DP=22 GT:GQ:DP 0/1:212:22 2 140 . A G 727 PASS DP=30 GT:GQ:DP 0/1:150:30 2 140 . GT G 727 PASS DP=30 GT:GQ:DP 0/1:150:30 2 150 . TAAAA TA,T 246 PASS DP=10 GT:GQ:DP 1/2:12:10 2 160 . TAAAA TA,TC,T 246 PASS DP=10 GT:GQ:DP 0/2:12:10 bcftools-1.2/test/concat.4.vcf.out000066400000000000000000000026431246371514100170760ustar00rootroot00000000000000##fileformat=VCFv4.0 ##FILTER= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= ##contig= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A 2 100 . GTTT G 1806 q10 DP=35 GT:GQ:DP 0/1:409:35 2 110 . CAAA C 1792 PASS DP=32 GT:GQ:DP 0/1:245:32 2 120 . GA G 628 q10 DP=21 GT:GQ:DP 1/1:21:21 2 130 . GAA G 1016 PASS DP=22 GT:GQ:DP 0/1:212:22 2 140 . A G 727 PASS DP=30 GT:GQ:DP 0/1:150:30 2 140 . GT G 727 PASS DP=30 GT:GQ:DP 0/1:150:30 2 150 . TAAAA TA,T 246 PASS DP=10 GT:GQ:DP 1/2:12:10 2 160 . TAAAA TA,TC,T 246 PASS DP=10 GT:GQ:DP 0/2:12:10 1 100 . GTTT G 1806 q10 DP=35 GT:GQ:DP 0/1:409:35 1 110 . C T,G 1792 Fail XX=11;DP=32 GT:GQ:DP 0/1:245:32 1 110 . CAAA C 1792 PASS DP=32 GT:GQ:DP 0/1:245:32 1 120 . GA G 628 q10 DP=21 GT:GQ:DP 1/1:21:21 1 130 . GAA GG 1016 PASS DP=22 GT:GQ:DP 0/1:212:22 1 130 . G T 1016 PASS DP=22 GT:GQ:DP 0/1:212:22 1 140 . GT G 727 PASS DP=30 GT:GQ:DP 0/1:150:30 1 150 . TAAAA TA,T 246 PASS DP=10 GT:GQ:DP 1/2:12:10 1 160 . TAAAA TA,T 246 PASS DP=10 GT:GQ:DP 1/2:12:10 bcftools-1.2/test/consensus.1.chain000066400000000000000000000002041246371514100173310ustar00rootroot00000000000000chain 497 1 501 + 1 501 1 502 + 1 502 1 11 3 1 1 0 3 485 chain 485 2 501 + 0 501 2 495 + 0 495 2 61 3 1 54 3 1 58 0 8 21 10 0 291 bcftools-1.2/test/consensus.1.out000066400000000000000000000020021246371514100170540ustar00rootroot00000000000000>1:2-501 TACAAAATATGATAAAATCAAAAAGAACATAACCTACGTATCAACTAAAGTGGTTGTTTG AAGAAAAGGAAGACTTAAAAAGAGTCAGTACTAACCTACATAATATATACAATGTTCATT AAATAATAAAATGAGCTCATCATACTTAGGTCATCATAAATATATCTGAAATTCACAAAT ATTGATCAAATGGTAAAATAGACAAGTAGATTTTAATAGGTTAAACAATTACTGATTCTC TTGAAAGAATAAATTTAATATGAGACCTATTTCATTATAATGAACTCACAAATTAGAAAC TTCACACTGGGGGCTGGAGAGATGGCTCAGTAGTTAAGAACACTGACTGCTCTTCTGAAG GTCCTGAGTTCAAATCCCAGCAACCACATGGTGACTTACAACCATCTGTAATGACATCTG NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN TTTAAAAACAAAAAAAAAGAA >2 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN AAATTAGTGATTTTCCATATTCTTTAAGTCATTTTAGAGTAATGTGTTCTTAAGATTTCA GAAAAACAAAAACTTGTGCTTTCCTGTTTGAAAAACAAACAGCTGTGGGGAATGGACGTA CGTTGTCGGGACAGCCTTTTTATAAAATAATGTTGAGGCTTTGATACGTCAAAGNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGCT GCTGCCAATGACAGCACACCCTGGGAATGCCCCAACTACTTACTACAAAGCAGTGTTACA TGGAGAAGATCTTCAAGAGTCTTTTTGCTAGATCTTTCCTTGGCTTTTGATGTGACTCCT CTCAATAAAATCCACAGTAATATAGTGAGTGGTCTCCTGCTCCAAACCAGTATTCCAGAC ACAGTTAATCCAGAC bcftools-1.2/test/consensus.2.chain000066400000000000000000000001521246371514100173340ustar00rootroot00000000000000chain 500 1 501 + 1 501 1 504 + 1 504 1 15 0 3 485 chain 491 2 501 + 0 501 2 491 + 0 491 2 200 10 0 291 bcftools-1.2/test/consensus.2.out000066400000000000000000000020001246371514100170530ustar00rootroot00000000000000>1:2-501 TACCATATGTGACATAAAATAAAAAAGAACATAACCTACGTATCAACTAAAGTGGTTGTT TGCAGAAAAGGAAGACTTAAAAAGAGTCAGTACTAACCTACATAATATATACAATGTTCA TTAAATAATAAAATGAGCTCATCATACTTAGGTCATCATAAATATATCTGAAATTCACAA ATATTGATCAAATGGTAAAATAGACAAGTAGATTTTAATAGGTTAAACAATTACTGATTC TCTTGAAAGAATAAATTTAATATGAGACCTATTTCATTATAATGAACTCACAAATTAGAA ACTTCACACTGGGGGCTGGAGAGATGGCTCAGTAGTTAAGAACACTGACTGCTCTTCTGA AGGTCCTGAGTTCAAATCCCAGCAACCACATGGTGACTTACAACCATCTGTAATGACATC TGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNTTTAAAAACAAAAAAAAAGAA >2 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN AGAGATTAGTGATTTTCCATATTCTTTAAGTCATTTTAGAGTAATGTGTTCTTAAGATAA ATCAGAAAAACAAAAACTTGTGCTTTCCTGTTTGAAAAACAAACAGCTGTGGGGAATGGT GTCGGGACAGCCTTTTTATAAAATAATGTTGAGGCTTTGATACGTCAAAGNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGCTGCTG CCAATGACAGCACACCCTGGGAATGCCCCAACTACTTACTACAAAGCAGTGTTACATGGA GAAGATCTTCAAGAGTCTTTTTGCTAGATCTTTCCTTGGCTTTTGATGTGACTCCTCTCA ATAAAATCCACAGTAATATAGTGAGTGGTCTCCTGCTCCAAACCAGTATTTCAGACACAG TTAATCCAGAC bcftools-1.2/test/consensus.3.chain000066400000000000000000000002041246371514100173330ustar00rootroot00000000000000chain 497 1 501 + 1 501 1 502 + 1 502 1 11 3 1 1 0 3 485 chain 485 2 501 + 0 501 2 495 + 0 495 2 61 3 1 54 3 1 58 0 8 21 10 0 291 bcftools-1.2/test/consensus.3.out000066400000000000000000000020021246371514100170560ustar00rootroot00000000000000>1:2-501 TACMAWATRTGATAAAATMAAAAAGAACATAACCTACGTATCAACTAAAGTGGTTGTTTG MAGAAAAGGAAGACTTAAAAAGAGTCAGTACTAACCTACATAATATATACAATGTTCATT AAATAATAAAATGAGCTCATCATACTTAGGTCATCATAAATATATCTGAAATTCACAAAT ATTGATCAAATGGTAAAATAGACAAGTAGATTTTAATAGGTTAAACAATTACTGATTCTC TTGAAAGAATAAATTTAATATGAGACCTATTTCATTATAATGAACTCACAAATTAGAAAC TTCACACTGGGGGCTGGAGAGATGGCTCAGTAGTTAAGAACACTGACTGCTCTTCTGAAG GTCCTGAGTTCAAATCCCAGCAACCACATGGTGACTTACAACCATCTGTAATGACATCTG NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN TTTAAAAACAAAAAAAAAGAA >2 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN AAATTAGTGATTTTCCATATTCTTTAAGTCATTTTAGAGTAATGTGTTCTTAAGATTTCA GAAAAACAAAAACTTGTGCTTTCCTGTTTGAAAAACAAACAGCTGTGGGGAATGGACGTA CGTTGTCGGGACAGCCTTTTTATAAAATAATGTTGAGGCTTTGATACGTCAAAGNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGCT GCTGCCAATGACAGCACACCCTGGGAATGCCCCAACTACTTACTACAAAGCAGTGTTACA TGGAGAAGATCTTCAAGAGTCTTTTTGCTAGATCTTTCCTTGGCTTTTGATGTGACTCCT CTCAATAAAATCCACAGTAATATAGTGAGTGGTCTCCTGCTCCAAACCAGTATTYCAGAC ACAGTTAATCCAGAC bcftools-1.2/test/consensus.4.chain000066400000000000000000000001521246371514100173360ustar00rootroot00000000000000chain 500 1 501 + 1 501 1 504 + 1 504 1 15 0 3 485 chain 491 2 501 + 0 501 2 491 + 0 491 2 200 10 0 291 bcftools-1.2/test/consensus.4.out000066400000000000000000000020001246371514100170550ustar00rootroot00000000000000>1:2-501 TACCATATGTGACATAAAATAAAAAAGAACATAACCTACGTATCAACTAAAGTGGTTGTT TGCAGAAAAGGAAGACTTAAAAAGAGTCAGTACTAACCTACATAATATATACAATGTTCA TTAAATAATAAAATGAGCTCATCATACTTAGGTCATCATAAATATATCTGAAATTCACAA ATATTGATCAAATGGTAAAATAGACAAGTAGATTTTAATAGGTTAAACAATTACTGATTC TCTTGAAAGAATAAATTTAATATGAGACCTATTTCATTATAATGAACTCACAAATTAGAA ACTTCACACTGGGGGCTGGAGAGATGGCTCAGTAGTTAAGAACACTGACTGCTCTTCTGA AGGTCCTGAGTTCAAATCCCAGCAACCACATGGTGACTTACAACCATCTGTAATGACATC TGATGCCCTCTGGTGTGTCTGAAGACAGCTACAGTGTACTTACATAAAATAATAAATAAA TCTTTAAAAACAAAAAAAAAGAA >2 GAAGATCTTTTCCTTATTAAGGATCTGAAGCTCTGTAGATTTGTATTCTATTAAACATGG AGAGATTAGTGATTTTCCATATTCTTTAAGTCATTTTAGAGTAATGTGTTCTTAAGATAA ATCAGAAAAACAAAAACTTGTGCTTTCCTGTTTGAAAAACAAACAGCTGTGGGGAATGGT GTCGGGACAGCCTTTTTATAAAATAATGTTGAGGCTTTGATACGTCAAAGTTATATTTCA AATGGAATCACTTAGACCTCGTTTCTGAGTGTCAATGGCCATATTGGGGATTTGCTGCTG CCAATGACAGCACACCCTGGGAATGCCCCAACTACTTACTACAAAGCAGTGTTACATGGA GAAGATCTTCAAGAGTCTTTTTGCTAGATCTTTCCTTGGCTTTTGATGTGACTCCTCTCA ATAAAATCCACAGTAATATAGTGAGTGGTCTCCTGCTCCAAACCAGTATTTCAGACACAG TTAATCCAGAC bcftools-1.2/test/consensus.fa000066400000000000000000000020071246371514100165010ustar00rootroot00000000000000>1:2-501 TACCATATGTGACATATAAAAAAGAACATAACCTACGTATCAACTAAAGTGGTTGTTTG CAGAAAAGGAAGACTTAAAAAGAGTCAGTACTAACCTACATAATATATACAATGTTCATT AAATAATAAAATGAGCTCATCATACTTAGGTCATCATAAATATATCTGAAATTCACAAAT ATTGATCAAATGGTAAAATAGACAAGTAGATTTTAATAGGTTAAACAATTACTGATTCTC TTGAAAGAATAAATTTAATATGAGACCTATTTCATTATAATGAACTCACAAATTAGAAAC TTCACACTGGGGGCTGGAGAGATGGCTCAGTAGTTAAGAACACTGACTGCTCTTCTGAAG GTCCTGAGTTCAAATCCCAGCAACCACATGGTGACTTACAACCATCTGTAATGACATCTG ATGCCCTCTGGTGTGTCTGAAGACAGCTACAGTGTACTTACATAAAATAATAAATAAATC TTTAAAAACAAAAAAAAAGAA >2 GAAGATCTTTTCCTTATTAAGGATCTGAAGCTCTGTAGATTTGTATTCTATTAAACATGG AGAGATTAGTGATTTTCCATATTCTTTAAGTCATTTTAGAGTAATGTGTTCTTAAGATAA ATCAGAAAAACAAAAACTTGTGCTTTCCTGTTTGAAAAACAAACAGCTGTGGGGAATGGT GTCGGGACAGCCTTTTTATAAAATTTTTCTAAATAATGTTGAGGCTTTGATACGTCAAAG TTATATTTCAAATGGAATCACTTAGACCTCGTTTCTGAGTGTCAATGGCCATATTGGGGA TTTGCTGCTGCCAATGACAGCACACCCTGGGAATGCCCCAACTACTTACTACAAAGCAGT GTTACATGGAGAAGATCTTCAAGAGTCTTTTTGCTAGATCTTTCCTTGGCTTTTGATGTG ACTCCTCTCAATAAAATCCACAGTAATATAGTGAGTGGTCTCCTGCTCCAAACCAGTATT TCAGACACAGTTAATCCAGAC bcftools-1.2/test/consensus.tab000066400000000000000000000000371246371514100166620ustar00rootroot000000000000001 421 480 2 1 60 2 241 300 bcftools-1.2/test/consensus.vcf000066400000000000000000000012241246371514100166710ustar00rootroot00000000000000##fileformat=VCFv4.2 ##FORMAT= ##INFO= ##ALT= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT NA001 1 5 . C a . PASS . GT 0/1 1 5 . C t . PASS . GT 0/1 1 7 . T a . PASS . GT . 1 10 . G a . PASS . GT 0/1 1 12 . GACA ga . PASS . GT 0/1 1 16 . T taaa . PASS . GT 1/1 1 19 . A c . PASS . GT 0/1 1 61 . C a . PASS . GT 0/1 2 61 . AGAG aa . PASS . GT 0/1 2 119 . AAA t . PASS . GT 0/1 2 179 . G gacgtacgt . PASS . GT 0/1 2 200 . A . PASS END=210 GT 1/0 2 481 . T c,a . PASS . GT 0/2 bcftools-1.2/test/convert.23andme000066400000000000000000000006701246371514100170100ustar00rootroot00000000000000# rsid chromosome position genotype rs001 1 2 AA rs002 1 10 AG rs003 1 14 AG rs004 1 24 TC rs005 1 44 CG rs006 1 53 GG rs007 1 60 GG rs008 1 62 CC rs009 1 75 AA rs010 1 80 GG rs011 1 89 TT rs012 1 96 -- rs013 1 99 CC rs014 1 102 GG rs015 1 112 TT rs016 2 5 CC rs017 2 11 CT rs018 2 16 CC rs019 2 20 GG rs020 2 33 CT rs021 2 39 AA rs022 2 44 CC rs023 2 48 CC rs024 2 55 AA rs025 2 59 CT rs026 Y 12 T rs027 Y 20 C bcftools-1.2/test/convert.23andme.vcf000066400000000000000000000017711246371514100175700ustar00rootroot00000000000000##fileformat=VCFv4.2 ##FILTER= ##contig= ##contig= ##contig= ##FORMAT= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT SAMPLE1 1 2 rs001 A . . . . GT 0/0 1 10 rs002 G A . . . GT 1/0 1 14 rs003 A G . . . GT 0/1 1 24 rs004 T C . . . GT 0/1 1 44 rs005 C G . . . GT 0/1 1 53 rs006 G . . . . GT 0/0 1 60 rs007 A G . . . GT 1/1 1 62 rs008 C . . . . GT 0/0 1 75 rs009 A . . . . GT 0/0 1 80 rs010 G . . . . GT 0/0 1 89 rs011 C T . . . GT 1/1 1 96 rs012 T . . . . GT . 1 99 rs013 C . . . . GT 0/0 1 102 rs014 T G . . . GT 1/1 1 112 rs015 T . . . . GT 0/0 2 5 rs016 C . . . . GT 0/0 2 11 rs017 T C . . . GT 1/0 2 16 rs018 C . . . . GT 0/0 2 20 rs019 G . . . . GT 0/0 2 33 rs020 T C . . . GT 1/0 2 39 rs021 A . . . . GT 0/0 2 44 rs022 C . . . . GT 0/0 2 48 rs023 C . . . . GT 0/0 2 55 rs024 C A . . . GT 1/1 2 59 rs025 C T . . . GT 0/1 Y 12 rs026 G T . . . GT 1 Y 20 rs027 C . . . . 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##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##reference=file:///illumina/scripts/clia/Genomes/Homo_sapiens/UCSC/hg19_rCRS/Sequence/WholeGenomeFasta/genome.fa ##contig= ##SnvTheta=0.001 ##IndelTheta=0.0001 ##MaxDepth_chr1=114.18 ##MaxDepth_chr10=131.73 ##MaxDepth_chr11=117.27 ##MaxDepth_chr12=116.97 ##MaxDepth_chr13=102.24 ##MaxDepth_chr14=101.55 ##MaxDepth_chr15=95.22 ##MaxDepth_chr16=111.33 ##MaxDepth_chr17=112.59 ##MaxDepth_chr18=121.86 ##MaxDepth_chr19=111.12 ##MaxDepth_chr2=121.83 ##MaxDepth_chr20=111.24 ##MaxDepth_chr21=98.43 ##MaxDepth_chr22=76.23 ##MaxDepth_chr3=120.09 ##MaxDepth_chr4=124.50 ##MaxDepth_chr5=119.82 ##MaxDepth_chr6=122.22 ##MaxDepth_chr7=120.27 ##MaxDepth_chr8=120.45 ##MaxDepth_chr9=102.48 ##MaxDepth_chrM=7005.66 ##MaxDepth_chrX=61.05 ##MaxDepth_chrY=37.17 ##FILTER= ##gvcftools_version="0.16" ##gvcftools_cmdline="/illumina/scripts/clia/workflows/IsisWorkflow/IsisWorkflow_v2.0.13/bin/set_haploid_region --ref /illumina/scripts/clia/Genomes/Homo_sapiens/UCSC/hg19_rCRS/Sequence/WholeGenomeFasta/genome.fa --region-file /illumina/scripts/clia/workflows/IsisWorkflow/IsisWorkflow_v2.0.13/bin/../data/het_mask/ncbi37/male.bed" ##FILTER= ##FORMAT= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT SAMPLE99 chr22 1 . N . 0 LowGQX END=16050039;BLOCKAVG_min30p3a GT:GQX:DP:DPF .:.:0:0 chr22 16050040 . C . 0 LowGQX END=16050050;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:5:2:0 chr22 16050051 . C . 0 LowGQX END=16050056;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:10:4:0 chr22 16050057 . C . 0 LowGQX END=16050072;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:15:6:0 chr22 16050073 . G . 0 LowGQX END=16050080;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:21:8:0 chr22 16050081 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:30:11:0 chr22 16050082 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:30:11:0 chr22 16050083 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:30:11:0 chr22 16050084 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:30:11:0 chr22 16050085 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:30:11:0 chr22 16050086 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:30:11:0 chr22 16050086 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:42:15:0 chr22 16050087 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:42:15:0 chr22 16050088 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:42:15:0 chr22 16050089 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:42:15:0 chr22 16050090 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:42:15:0 chr22 16050091 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:42:15:0 chr22 16050092 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:42:15:0 chr22 16050093 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:42:15:0 chr22 16050094 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:42:15:0 chr22 16050095 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:42:15:0 chr22 16050096 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:42:15:0 chr22 16050097 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:42:15:0 chr22 16050098 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:42:15:0 chr22 16050099 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:42:15:0 chr22 16050100 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:42:15:0 chr22 16050101 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:42:15:0 chr22 16050102 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:42:15:0 chr22 16050103 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:42:15:0 chr22 16050103 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:54:19:0 chr22 16050104 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:54:19:0 chr22 16050105 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:54:19:0 chr22 16050106 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:54:19:0 chr22 16050107 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:54:19:0 chr22 16050108 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:54:19:0 chr22 16050109 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:54:19:0 chr22 16050110 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:54:19:0 chr22 16050111 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:54:19:0 chr22 16050112 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:54:19:0 chr22 16050113 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:54:19:0 chr22 16050114 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:54:19:0 chr22 16050115 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:54:19:0 chr22 16050116 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:54:19:0 chr22 16050116 . G C 23 LowGQX SNVSB=0;SNVHPOL=2 GT:GQ:GQX:DP:DPF:AD 0/1:56:23:22:0:16,6 chr22 16050117 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:69:24:0 chr22 16050118 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:69:24:0 chr22 16050119 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:69:24:0 chr22 16050120 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:69:24:0 chr22 16050121 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:69:24:0 chr22 16050122 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:69:24:0 chr22 16050123 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:69:24:0 chr22 16050124 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:69:24:0 chr22 16050125 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:69:24:0 chr22 16050126 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:69:24:0 chr22 16050127 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:69:24:0 chr22 16050128 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:69:24:0 chr22 16050129 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:69:24:0 chr22 16050130 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:69:24:0 chr22 16050131 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:69:24:0 chr22 16050132 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:69:24:0 chr22 16050132 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050133 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050134 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050135 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050136 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050137 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050138 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050139 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050140 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050141 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050142 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050143 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050144 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050145 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050146 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050147 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050148 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050149 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050150 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050151 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050152 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050153 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050154 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050155 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050156 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050157 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050158 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050159 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050160 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050161 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050162 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050163 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050164 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050165 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050166 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050167 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050168 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050169 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050170 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050171 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:84:29:0 chr22 16050171 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050172 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050173 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050174 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050175 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050176 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050177 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050178 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050179 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050180 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050181 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050182 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050183 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050184 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050185 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050186 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050187 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050188 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050189 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050190 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050191 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050192 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050193 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050194 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050195 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050196 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050197 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050198 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050199 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050200 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050201 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050202 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050203 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050204 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050205 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050206 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050207 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050208 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050209 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050210 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050211 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050212 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050213 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050214 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050215 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050216 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050216 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:93:32:2 chr22 16050217 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:93:32:2 chr22 16050218 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:93:32:2 chr22 16050218 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050219 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050220 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050221 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050222 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050223 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050224 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050225 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050226 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050227 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050228 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050229 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050230 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050231 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050232 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050233 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050234 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050235 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050235 . T . 0 PASS . GT:GQX:DP:DPF 0/0:72:36:0 chr22 16050236 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050237 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050238 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050239 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050240 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050241 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050242 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050243 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050244 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050245 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050246 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050247 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050248 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050249 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050250 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050251 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050252 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050253 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050254 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050255 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050256 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050257 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050258 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050259 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050260 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050261 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050262 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050262 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050263 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050264 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050265 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050266 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050267 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050268 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050269 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050270 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050271 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050272 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050273 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050274 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050275 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050276 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050277 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050278 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050279 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050280 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050280 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050281 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050282 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050283 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050284 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050285 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050286 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050287 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050288 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050288 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:114:39:0 chr22 16050289 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:114:39:0 chr22 16050290 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:114:39:0 chr22 16050291 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:114:39:0 chr22 16050292 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:114:39:0 chr22 16050293 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:114:39:0 chr22 16050294 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:114:39:0 chr22 16050295 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:114:39:0 chr22 16050296 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:114:39:0 chr22 16050297 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:114:39:0 chr22 16050298 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:114:39:0 chr22 16050299 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:114:39:0 chr22 16050300 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:114:39:0 chr22 16050300 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:150:51:0 chr22 16050301 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:150:51:0 chr22 16050302 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:150:51:0 chr22 16050303 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:150:51:0 chr22 16050304 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:150:51:0 chr22 16050305 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:150:51:0 chr22 16050306 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:150:51:0 chr22 16050307 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:150:51:0 chr22 16050308 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:150:51:0 chr22 16050309 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:150:51:0 chr22 16050310 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:150:51:0 chr22 16050310 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050311 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050312 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050313 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050314 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050315 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050316 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050317 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050318 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050319 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050320 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050321 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050322 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050323 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050324 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050325 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050326 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050327 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050328 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050329 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050330 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050331 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050332 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050333 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050334 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050335 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050336 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050337 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050338 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050339 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050340 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050341 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050342 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050343 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050344 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050345 . A . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050345 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:220:74:0 chr22 16050346 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:220:74:0 chr22 16050347 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:220:74:0 chr22 16050347 . T . 0 HighDepth END=16050372;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:229:77:0 chr22 16050373 . T . 0 HighDepth END=16050407;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:241:81:0 chr22 16050408 . T . 0 HighDepth END=16050414;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:200:75:0 chr22 16050415 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:217:73:0 chr22 16050416 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:217:73:0 chr22 16050417 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:217:73:0 chr22 16050418 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:217:73:0 chr22 16050419 . G . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:217:73:0 chr22 16050419 . C . 0 HighDepth END=16050420;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:223:75:0 chr22 16050421 . T . 0 PASS . GT:GQX:DP:DPF 0/0:223:75:1 chr22 16050422 . T . 0 HighDepth END=16050427;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:223:75:0 chr22 16050428 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050429 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050430 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050431 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050432 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050433 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050434 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050435 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050436 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050437 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050438 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050439 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050440 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050441 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050442 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050443 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050444 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050445 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050446 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050447 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050448 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050449 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050450 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050451 . T . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050451 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:142:54:0 chr22 16050452 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:142:54:0 chr22 16050453 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:142:54:0 chr22 16050454 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:142:54:0 chr22 16050455 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:142:54:0 chr22 16050456 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:142:54:0 chr22 16050457 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:142:54:0 chr22 16050458 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:142:54:0 chr22 16050459 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:142:54:0 chr22 16050460 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:142:54:0 chr22 16050461 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:142:54:0 chr22 16050462 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:142:54:0 chr22 16050463 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:142:54:0 chr22 16050464 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:142:54:0 chr22 16050465 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:142:54:0 chr22 16050466 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:142:54:0 chr22 16050467 . C . 0 PASS BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:142:54:0 bcftools-1.2/test/convert.gvcf.vcf000066400000000000000000000237651246371514100172730ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##reference=file:///illumina/scripts/clia/Genomes/Homo_sapiens/UCSC/hg19_rCRS/Sequence/WholeGenomeFasta/genome.fa ##contig= ##SnvTheta=0.001 ##IndelTheta=0.0001 ##MaxDepth_chr1=114.18 ##MaxDepth_chr10=131.73 ##MaxDepth_chr11=117.27 ##MaxDepth_chr12=116.97 ##MaxDepth_chr13=102.24 ##MaxDepth_chr14=101.55 ##MaxDepth_chr15=95.22 ##MaxDepth_chr16=111.33 ##MaxDepth_chr17=112.59 ##MaxDepth_chr18=121.86 ##MaxDepth_chr19=111.12 ##MaxDepth_chr2=121.83 ##MaxDepth_chr20=111.24 ##MaxDepth_chr21=98.43 ##MaxDepth_chr22=76.23 ##MaxDepth_chr3=120.09 ##MaxDepth_chr4=124.50 ##MaxDepth_chr5=119.82 ##MaxDepth_chr6=122.22 ##MaxDepth_chr7=120.27 ##MaxDepth_chr8=120.45 ##MaxDepth_chr9=102.48 ##MaxDepth_chrM=7005.66 ##MaxDepth_chrX=61.05 ##MaxDepth_chrY=37.17 ##FILTER= ##gvcftools_version="0.16" ##gvcftools_cmdline="/illumina/scripts/clia/workflows/IsisWorkflow/IsisWorkflow_v2.0.13/bin/set_haploid_region --ref /illumina/scripts/clia/Genomes/Homo_sapiens/UCSC/hg19_rCRS/Sequence/WholeGenomeFasta/genome.fa --region-file /illumina/scripts/clia/workflows/IsisWorkflow/IsisWorkflow_v2.0.13/bin/../data/het_mask/ncbi37/male.bed" ##FILTER= ##FORMAT= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT SAMPLE99 chr22 1 . 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G . 0 PASS END=16050215;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:90:31:0 chr22 16050216 . T . 0 PASS END=16050217;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:93:32:2 chr22 16050218 . T . 0 PASS END=16050234;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:102:35:0 chr22 16050235 . T . 0 PASS . GT:GQX:DP:DPF 0/0:72:36:0 chr22 16050236 . T . 0 PASS END=16050261;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050262 . A . 0 PASS END=16050279;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:66:23:0 chr22 16050280 . G . 0 PASS END=16050287;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:87:30:0 chr22 16050288 . A . 0 PASS END=16050299;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:114:39:0 chr22 16050300 . G . 0 PASS END=16050309;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:150:51:0 chr22 16050310 . A . 0 PASS END=16050344;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:166:63:0 chr22 16050345 . C . 0 PASS END=16050346;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:220:74:0 chr22 16050347 . T . 0 HighDepth END=16050372;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:229:77:0 chr22 16050373 . T . 0 HighDepth END=16050407;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:241:81:0 chr22 16050408 . T . 0 HighDepth END=16050414;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:200:75:0 chr22 16050415 . G . 0 PASS END=16050418;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:217:73:0 chr22 16050419 . C . 0 HighDepth END=16050420;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:223:75:0 chr22 16050421 . T . 0 PASS . GT:GQX:DP:DPF 0/0:223:75:1 chr22 16050422 . T . 0 HighDepth END=16050427;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:223:75:0 chr22 16050428 . T . 0 PASS END=16050450;BLOCKAVG_min30p3a GT:GQX:DP:DPF 0/0:178:60:0 chr22 16050451 . 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TACACACAC T 59.9 PASS AN=4;AC=2 GT 0/1 0/1 bcftools-1.2/test/filter.3.out000066400000000000000000000000241246371514100163250ustar00rootroot000000000000003162007 q20 . 0/1 2 bcftools-1.2/test/filter.3.vcf000066400000000000000000000071341246371514100163050ustar00rootroot00000000000000##fileformat=VCFv4.2 ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##SAMPLE=\") quoting nonsense where double brackets would do just fine",softwareName=,softwareVer=<119,65>,softwareParam=<.>,MetadataResource=http://somewhere.com/path> ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3162006 . GAA G,GA 238 q20 DP=19;AN=4;AC=1,1;XRF=1e6,2e6,3e6;XRI=1111,2222,3333;XRS=ABC,DEF,GHI;XAF=1e6,2e6;XAI=1111,2222;XAS=ABC,DEF;XGF=1e6,2e6,3e6,4e6,5e6,6e6;XGI=11,22,33,44,55,66;XGS=ABC,DEF,GHI,JKL,MNO,PQR;TXT=ABC,DEF,GHI GT:GQ:DP:STR 0/1:589:19:XX 0/2:1:1:YY 1 3162007 . TAGGG CAGGG,CAGGT 238 q20 AO=52101,113;CIGAR=1X4M,1X3M1X;TXT0=text GT:FGS:FGI:FGF:FAS:FAI:FAF:FRS:FRI:FRF 0/1:AAAAAA,BBBBB,CCCC,DDD,EE,F:1,2,3,4,5,6:1e-1,2e-2,3e-3,4e-4,5e-5,6e-6:AAA,B:1,2:1e-1,2e-2:A,BB,CCC:1,2,3:1e-1,2e-2,3e-3 2:AAAAAA,BBB,C:1,2,3:1e-1,2e-2,3e-3:AAA,B:1,2:1e-1,2e-2:A,BB,CCC:1,2,3:1e-1,2e-2,3e-3 bcftools-1.2/test/filter.4.out000066400000000000000000000000521246371514100163270ustar00rootroot000000000000003162006 XX 19 0/1 0/2 3162007 q20 . 0/1 2 bcftools-1.2/test/filter.5.out000066400000000000000000000000561246371514100163340ustar00rootroot000000000000003162006 q20;XX 19 0/1 0/2 3162007 q20 . 0/1 2 bcftools-1.2/test/filter.6.out000066400000000000000000000000531246371514100163320ustar00rootroot000000000000003162006 XX 19 0/1 0/2 3162007 PASS . 0/1 2 bcftools-1.2/test/filter.7.out000066400000000000000000000000571246371514100163370ustar00rootroot000000000000003162006 q20;XX 19 0/1 0/2 3162007 PASS . 0/1 2 bcftools-1.2/test/filter.8.out000066400000000000000000000000221246371514100163300ustar00rootroot000000000000003177144 2 0/0 1/1 bcftools-1.2/test/fixploidy.out000066400000000000000000000023051246371514100167120ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B C 1 3000001 xx C CT 11 PASS FLAG;IINT=11;IFLT=1.1;ISTR=xxx GT:FINT:FFLT:FSTR .:11:1.1:xxx 0:11:1.1:x 0|0:11:1.1:x 1 3000002 . C CTT . . . GT ././. ./././. .|.|.|.|. 1 3000003 xx C CTTT 11 q11 FLAG;IINT=.;IFLT=.;ISTR=. GT:FINT:FFLT:FSTR 0/0:.:.:. 0/0:.:.:. 0|0:.:.:. 1 3000004 xx C CTTTT 11 q11 FLAG;IINT=11;IFLT=1.1;ISTR=xxx GT:FINT:FFLT:FSTR 0/0/0/0/0/0:11:1.1:x 0/0/0/0/0/0/0:11:1.1:xxx 0|0|0|0|0|0|0|0:11:1.1:xxx bcftools-1.2/test/fixploidy.ploidy000066400000000000000000000003301246371514100173770ustar00rootroot000000000000001 3000001 3000001 X 0 1 3000001 3000001 Y 1 1 3000001 3000001 Z 2 1 3000002 3000002 X 3 1 3000002 3000002 Y 4 1 3000002 3000002 Z 5 1 3000004 3000004 X 6 1 3000004 3000004 Y 7 1 3000004 3000004 Z 8 bcftools-1.2/test/fixploidy.samples000066400000000000000000000000141246371514100175420ustar00rootroot00000000000000A X B Y C Z bcftools-1.2/test/fixploidy.vcf000066400000000000000000000021531246371514100166620ustar00rootroot00000000000000##fileformat=VCFv4.1 ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B C 1 3000001 xx C CT 11 PASS FLAG;IINT=11;IFLT=1.1;ISTR=xxx GT:FINT:FFLT:FSTR 0/0:11:1.1:xxx 0/0:11:1.1:x 0|0:11:1.1:x 1 3000002 . C CTT . . . GT ./. ./. .|. 1 3000003 xx C CTTT 11 q11 FLAG;IINT=.;IFLT=.;ISTR=. GT:FINT:FFLT:FSTR 0/0:.:.:. 0/0:.:.:. 0|0:.:.:. 1 3000004 xx C CTTTT 11 q11 FLAG;IINT=11;IFLT=1.1;ISTR=xxx GT:FINT:FFLT:FSTR 0/0:11:1.1:x 0/0:11:1.1:xxx 0|0:11:1.1:xxx bcftools-1.2/test/idx.out000066400000000000000000000000701246371514100154640ustar00rootroot0000000000000011 135006516 2 20 63025520 7 X 155270560 4 Y 59373566 2 bcftools-1.2/test/idx.vcf000066400000000000000000000012011246371514100154300ustar00rootroot00000000000000##fileformat=VCFv4.1 ##contig= ##contig= ##contig= ##contig= ##contig= #CHROM POS ID REF ALT QUAL FILTER INFO 11 2343543 . A . 999 PASS . 11 5464562 . C T 999 PASS . 20 76962 . T C 999 PASS . 20 126310 . ACC A 999 PASS . 20 138125 . G T 999 PASS . 20 138148 . C T 999 PASS . 20 271225 . T TTTA,TA 999 PASS . 20 304568 . C T 999 PASS . 20 326891 . A AC 999 PASS . X 2928329 . C T 999 PASS . X 2933066 . G C 999 PASS . X 2942109 . T C 999 PASS . X 3048719 . T C 999 PASS . Y 8657215 . C A 999 PASS . Y 10011673 . G A 999 PASS . bcftools-1.2/test/idx_count.out000066400000000000000000000000031246371514100166700ustar00rootroot0000000000000015 bcftools-1.2/test/isec.a.vcf000066400000000000000000000033031246371514100160130ustar00rootroot00000000000000##fileformat=VCFv4.1 ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##test= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= ##readme=AAAAAA ##readme=BBBBBB #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A 1 3062915 . GTTT G 1806 q10 DP=35;DP4=1,2,3,4;AN=2;AC=1 GT:GQ:DP:GL 0/1:409:35:-20,-5,-20 1 3062915 . G T 1806 q10 DP=35;DP4=1,2,3,4;AN=2;AC=1 GT:GQ:DP:GL 0/1:409:35:-20,-5,-20 1 3106154 . CAAA C 1792 PASS DP=32;AN=2;AC=1 GT:GQ:DP 0/1:245:32 1 3106154 . C T,CT 1792 PASS DP=32;AN=2;AC=1 GT:GQ:DP 0/1:245:32 1 3157410 . GA G 628 q10 DP=21;AN=2;AC=2 GT:GQ:DP 1/1:21:21 1 3162006 . GAA G 1016 PASS DP=22;AN=2;AC=1 GT:GQ:DP 0/1:212:22 1 3177144 . GT G 727 PASS DP=30;AN=2;AC=1 GT:GQ:DP 0/1:150:30 1 3184885 . TAAAA TA,T 246 PASS DP=10;AN=2;AC=1,1 GT:GQ:DP 1/2:12:10 2 3199812 . G GTT,GT 481 PASS DP=26;AN=2;AC=1,1 GT:GQ:DP 1/2:322:26 3 3212016 . CTT C,CT 565 PASS DP=26;AN=2;AC=1,1 GT:GQ:DP 1/2:91:26 4 3212016 . TACACACAC T 325 PASS DP=31;AN=2;AC=1 GT:GQ:DP 0/1:325:31 4 3258448 . TACACACAC T 325 PASS DP=31;AN=2;AC=1 GT:GQ:DP 0/1:325:31 bcftools-1.2/test/isec.ab.C.out000066400000000000000000000001051246371514100163640ustar00rootroot000000000000002 3199812 G GTT,GT 10 3 3212016 CTT C,CT 10 4 3258448 TACACACAC T 10 bcftools-1.2/test/isec.ab.any.out000066400000000000000000000002201246371514100167670ustar00rootroot000000000000001 3062915 GTTT G 11 1 3106154 CAAA C 11 1 3157410 GA G 11 1 3162006 GAA G 11 1 3177144 GT G 11 1 3184885 TAAAA TA,T 11 4 3212016 TACACACAC T 11 bcftools-1.2/test/isec.ab.both.out000066400000000000000000000002411246371514100171370ustar00rootroot000000000000001 3062915 GTTT G 11 1 3062915 G T 11 1 3106154 CAAA C 11 1 3157410 GA G 11 1 3162006 GAA G 11 1 3177144 GT G 11 1 3184885 TAAAA TA,T 11 4 3212016 TACACACAC T 11 bcftools-1.2/test/isec.ab.flt.out000066400000000000000000000000441246371514100167710ustar00rootroot000000000000001 3157410 GA G 11 1 3177144 GT G 11 bcftools-1.2/test/isec.ab.out000066400000000000000000000000671246371514100162120ustar00rootroot000000000000001 3157410 GA G 11 1 3162006 GAA G 11 1 3177144 GT G 11 bcftools-1.2/test/isec.b.vcf000066400000000000000000000034051246371514100160170ustar00rootroot00000000000000##fileformat=VCFv4.1 ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT B 1 3062915 . G A 376 q20 DP=14;DP4=1,2,3,4;AN=2;AC=1 GT:GQ:DP:GL 0/1:376:14:-10,0,-10 1 3062915 . GTTT GT 376 q20 DP=14;DP4=1,2,3,4;AN=2;AC=1 GT:GQ:DP:GL 0/1:376:14:-10,0,-10 1 3106154 . C T 677 PASS DP=15;AN=2;AC=1 GT:GQ:DP:GL 0/1:277:15:-10,0,-10 1 3106154 . CAAAA C 677 PASS DP=15;AN=2;AC=1 GT:GQ:DP:GL 0/1:277:15:-10,0,-10 1 3157410 . GA G 249 PASS DP=11;AN=2;AC=1 GT:GQ:DP 0/1:49:11 1 3162006 . GAA G 663 PASS DP=19;AN=2;AC=1 GT:GQ:DP 0/1:589:19 1 3177144 . GT G 460 PASS DP=24;AN=2;AC=1 GT:GQ:DP 0/1:236:24 1 3184885 . TAAA T 598 PASS DP=16;AN=2;AC=1 GT:GQ:DP 0/1:435:16 2 3188209 . GA G 162 . DP=15;AN=2;AC=1 GT:GQ:DP 0/1:162:15 3 3199812 . G GTT,GT 353 PASS DP=19;AN=2;AC=1,1 GT:GQ:DP 1/2:188:19 4 3212016 . CTT C 677 q20 DP=15;AN=2;AC=1 GT:GQ:DP 0/1:158:15 bcftools-1.2/test/isec.tab000066400000000000000000000001321246371514100155610ustar00rootroot000000000000001 3062915 3062915 1 3106154 3106154 1 3157410 3162006 1 3177144 3177144 4 3212016 3212016 bcftools-1.2/test/isec.tab.out000066400000000000000000000030461246371514100163760ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##test= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= ##readme=AAAAAA ##readme=BBBBBB ##contig= ##contig= ##contig= ##contig= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A 1 3062915 . GTTT G 1806 q10 DP=35;DP4=1,2,3,4;AN=2;AC=1 GT:GQ:DP:GL 0/1:409:35:-20,-5,-20 1 3062915 . G T 1806 q10 DP=35;DP4=1,2,3,4;AN=2;AC=1 GT:GQ:DP:GL 0/1:409:35:-20,-5,-20 1 3106154 . CAAA C 1792 PASS DP=32;AN=2;AC=1 GT:GQ:DP 0/1:245:32 1 3106154 . C T,CT 1792 PASS DP=32;AN=2;AC=1 GT:GQ:DP 0/1:245:32 1 3157410 . GA G 628 q10 DP=21;AN=2;AC=2 GT:GQ:DP 1/1:21:21 1 3162006 . GAA G 1016 PASS DP=22;AN=2;AC=1 GT:GQ:DP 0/1:212:22 1 3177144 . GT G 727 PASS DP=30;AN=2;AC=1 GT:GQ:DP 0/1:150:30 4 3212016 . TACACACAC T 325 PASS DP=31;AN=2;AC=1 GT:GQ:DP 0/1:325:31 bcftools-1.2/test/large_chrom.20.1.2147483647.out000066400000000000000000000004401246371514100206360ustar00rootroot00000000000000chr20 76962 . T C 999 PASS . chr20 126310 . ACC A 999 PASS . chr20 138125 . G T 999 PASS . chr20 138148 . C T 999 PASS . chr20 271225 . T TTTA,TA 999 PASS . chr20 304568 . C T 999 PASS . chr20 620255100 . AG T 999 PASS . chr20 630255200 . G C 999 PASS . chr20 2147483647 . A T 999 PASS . bcftools-1.2/test/large_chrom_csi_limit.20.1.2147483647.out000066400000000000000000000004401246371514100226720ustar00rootroot00000000000000chr20 76962 . T C 999 PASS . chr20 126310 . ACC A 999 PASS . chr20 138125 . G T 999 PASS . chr20 138148 . C T 999 PASS . chr20 271225 . T TTTA,TA 999 PASS . chr20 304568 . C T 999 PASS . chr20 620255100 . AG T 999 PASS . chr20 630255200 . G C 999 PASS . chr20 2147483647 . A T 999 PASS . bcftools-1.2/test/large_chrom_csi_limit.vcf000066400000000000000000000011561246371514100211730ustar00rootroot00000000000000##fileformat=VCFv4.2 ##reference=file:///seq/references/long_chrom.fasta ##FILTER= ##contig= ##contig= #CHROM POS ID REF ALT QUAL FILTER INFO chr11 2343543 . A . 999 PASS . chr11 5464562 . C T 999 PASS . chr11 116870911 . C G 999 PASS . chr20 76962 . T C 999 PASS . chr20 126310 . ACC A 999 PASS . chr20 138125 . G T 999 PASS . chr20 138148 . C T 999 PASS . chr20 271225 . T TTTA,TA 999 PASS . chr20 304568 . C T 999 PASS . chr20 620255100 . AG T 999 PASS . chr20 630255200 . G C 999 PASS . chr20 2147483647 . A T 999 PASS . bcftools-1.2/test/large_chrom_tbi_limit.20.1.536870912.out000066400000000000000000000001371246371514100226100ustar00rootroot00000000000000chr11 2343543 . A . 999 PASS . chr11 5464562 . C T 999 PASS . chr11 116870911 . C G 999 PASS . bcftools-1.2/test/large_chrom_tbi_limit.vcf000066400000000000000000000013351246371514100211720ustar00rootroot00000000000000##fileformat=VCFv4.2 ##reference=file:///seq/references/long_chrom.fasta ##FILTER= ##FILTER= ##contig= ##contig= #CHROM POS ID REF ALT QUAL FILTER INFO chr11 2343543 . A . 999 PASS . chr11 5464562 . C T 999 PASS . chr11 116870911 . C G 999 PASS . chr20 76962 . T C 999 PASS . chr20 126310 . ACC A 999 PASS . chr20 138125 . G T 999 PASS . chr20 138148 . C T 999 PASS . chr20 271225 . T TTTA,TA 999 PASS . chr20 304568 . C T 999 PASS . chr20 220255100 . AG T 999 PASS . chr20 230255200 . G C 999 PASS . chr20 536870912 . A T 999 PASS . chr20 536870913 . A T 999 TABIX . bcftools-1.2/test/merge.2.a.vcf000066400000000000000000000036151246371514100163350ustar00rootroot00000000000000##fileformat=VCFv4.1 ##INFO= ##FORMAT= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3000000 . C CCG 59.2 PASS AN=4;AC=2 GT:GQ 0/1:245 0/1:245 1 3000150 . C A 59.2 PASS AN=4;AC=2 GT:GQ 0/1:245 0/1:245 1 3000151 . C A 59.2 PASS AN=4;AC=2 GT:DP:GQ 0/1:32:245 0/1:32:245 1 3106154 . C CC 342 PASS AN=4;AC=2 GT:GQ:DP 0/1:245:32 0/1:245:32 1 3106154 . C A 59.2 PASS AN=4;AC=2 GT:GQ:DP 0/1:245:32 0/1:245:32 1 3200000 . C T 59.2 PASS AN=4;AC=2 GT:GQ:DP 0/1:245:32 0/1:245:32 1 3200010 . C T 59.2 PASS AN=4;AC=2 GT:GQ:DP 0/1:245:32 0/1:245:32 1 3200020 . C G,T 59.2 PASS AN=4;AC=2 GT:GL ./.:1,2,3,4,5,6 .:1,2,3 bcftools-1.2/test/merge.2.all.out000066400000000000000000000042131246371514100167110ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 2:A 2:B 1 3000000 . C CCG,G 59.2 PASS AN=8;AC=2,2 GT:GQ 0/1:245 0/1:245 0/2:245 0/2:245 1 3000150 . C A,G 59.2 PASS AN=8;AC=2,2 GT:GQ 0/1:245 0/1:245 0/2:245 0/2:245 1 3000151 . C A,G 59.2 PASS AN=8;AC=2,2 GT:DP:GQ 0/1:32:245 0/1:32:245 0/2:32:245 0/2:32:245 1 3106154 . C CC,CCC 342 PASS AN=8;AC=2,2 GT:GQ:DP 0/1:245:32 0/1:245:32 0/2:245:32 0/2:245:32 1 3106154 . C A,T 59.2 PASS AN=8;AC=2,2 GT:GQ:DP 0/1:245:32 0/1:245:32 0/2:245:32 0/2:245:32 1 3200000 . C T 59.2 PASS AN=8;AC=4 GT:GQ:DP 0/1:245:32 0/1:245:32 0/1:245:32 0/1:245:32 1 3200010 . C T,A 59.2 PASS AN=8;AC=2,2 GT:GQ:DP 0/1:245:32 0/1:245:32 0/2:245:32 0/2:245:32 1 3200020 . C G,T 59.2 PASS AN=0;AC=0,0 GT:GL ./.:1,2,3,4,5,6 .:1,2,3 ./.:1,2,3,4,5,6 .:1,2,3 bcftools-1.2/test/merge.2.b.vcf000066400000000000000000000036161246371514100163370ustar00rootroot00000000000000##fileformat=VCFv4.1 ##INFO= ##FORMAT= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3000000 . C G 59.2 PASS AN=4;AC=2 GT:GQ 0/1:245 0/1:245 1 3000150 . C G 59.2 PASS AN=4;AC=2 GT:GQ 0/1:245 0/1:245 1 3000151 . C G 59.2 PASS AN=4;AC=2 GT:DP:GQ 0/1:32:245 0/1:32:245 1 3106154 . C CCC 342 PASS AN=4;AC=2 GT:GQ:DP 0/1:245:32 0/1:245:32 1 3106154 . C T 59.2 PASS AN=4;AC=2 GT:GQ:DP 0/1:245:32 0/1:245:32 1 3200000 . C T 59.2 PASS AN=4;AC=2 GT:GQ:DP 0/1:245:32 0/1:245:32 1 3200010 . C A,T 59.2 PASS AN=4;AC=2 GT:GQ:DP 0/1:245:32 0/1:245:32 1 3200020 . C T,G 59.2 PASS AN=4;AC=2 GT:GL ./.:1,4,6,2,5,3 .:1,3,2 bcftools-1.2/test/merge.2.both.out000066400000000000000000000043111246371514100170740ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 2:A 2:B 1 3000000 . C CCG 59.2 PASS AN=4;AC=2 GT:GQ 0/1:245 0/1:245 ./.:. ./.:. 1 3000000 . C G 59.2 PASS AN=4;AC=2 GT:GQ ./.:. ./.:. 0/1:245 0/1:245 1 3000150 . C A,G 59.2 PASS AN=8;AC=2,2 GT:GQ 0/1:245 0/1:245 0/2:245 0/2:245 1 3000151 . C A,G 59.2 PASS AN=8;AC=2,2 GT:DP:GQ 0/1:32:245 0/1:32:245 0/2:32:245 0/2:32:245 1 3106154 . C CC,CCC 342 PASS AN=8;AC=2,2 GT:GQ:DP 0/1:245:32 0/1:245:32 0/2:245:32 0/2:245:32 1 3106154 . C A,T 59.2 PASS AN=8;AC=2,2 GT:GQ:DP 0/1:245:32 0/1:245:32 0/2:245:32 0/2:245:32 1 3200000 . C T 59.2 PASS AN=8;AC=4 GT:GQ:DP 0/1:245:32 0/1:245:32 0/1:245:32 0/1:245:32 1 3200010 . C T,A 59.2 PASS AN=8;AC=2,2 GT:GQ:DP 0/1:245:32 0/1:245:32 0/2:245:32 0/2:245:32 1 3200020 . C G,T 59.2 PASS AN=0;AC=0,0 GT:GL ./.:1,2,3,4,5,6 .:1,2,3 ./.:1,2,3,4,5,6 .:1,2,3 bcftools-1.2/test/merge.2.none.out000066400000000000000000000047411246371514100171060ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 2:A 2:B 1 3000000 . C CCG 59.2 PASS AN=4;AC=2 GT:GQ 0/1:245 0/1:245 ./.:. ./.:. 1 3000000 . C G 59.2 PASS AN=4;AC=2 GT:GQ ./.:. ./.:. 0/1:245 0/1:245 1 3000150 . C A 59.2 PASS AN=4;AC=2 GT:GQ 0/1:245 0/1:245 ./.:. ./.:. 1 3000150 . C G 59.2 PASS AN=4;AC=2 GT:GQ ./.:. ./.:. 0/1:245 0/1:245 1 3000151 . C A 59.2 PASS AN=4;AC=2 GT:DP:GQ 0/1:32:245 0/1:32:245 ./.:.:. ./.:.:. 1 3000151 . C G 59.2 PASS AN=4;AC=2 GT:DP:GQ ./.:.:. ./.:.:. 0/1:32:245 0/1:32:245 1 3106154 . C CC 342 PASS AN=4;AC=2 GT:GQ:DP 0/1:245:32 0/1:245:32 ./.:.:. ./.:.:. 1 3106154 . C A 59.2 PASS AN=4;AC=2 GT:GQ:DP 0/1:245:32 0/1:245:32 ./.:.:. ./.:.:. 1 3106154 . C CCC 342 PASS AN=4;AC=2 GT:GQ:DP ./.:.:. ./.:.:. 0/1:245:32 0/1:245:32 1 3106154 . C T 59.2 PASS AN=4;AC=2 GT:GQ:DP ./.:.:. ./.:.:. 0/1:245:32 0/1:245:32 1 3200000 . C T 59.2 PASS AN=8;AC=4 GT:GQ:DP 0/1:245:32 0/1:245:32 0/1:245:32 0/1:245:32 1 3200010 . C T,A 59.2 PASS AN=8;AC=2,2 GT:GQ:DP 0/1:245:32 0/1:245:32 0/2:245:32 0/2:245:32 1 3200020 . C G,T 59.2 PASS AN=0;AC=0,0 GT:GL ./.:1,2,3,4,5,6 .:1,2,3 ./.:1,2,3,4,5,6 .:1,2,3 bcftools-1.2/test/merge.3.a.vcf000066400000000000000000000032031246371514100163270ustar00rootroot00000000000000##fileformat=VCFv4.1 ##INFO= ##FORMAT= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3000000 . C CCG 59.2 PASS AN=4;AC=2;TR=1,2;TA=1;TG=1,2,3 GT:GQ 0/1:245 0/1:245 bcftools-1.2/test/merge.3.b.vcf000066400000000000000000000032101246371514100163260ustar00rootroot00000000000000##fileformat=VCFv4.1 ##INFO= ##FORMAT= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3000000 . C CG 59.2 PASS AN=4;AC=2;TR=10,20;TA=10;TG=10,20,30 GT:GQ 0/1:245 0/1:245 bcftools-1.2/test/merge.3.out000066400000000000000000000033441246371514100161470ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 2:A 2:B 1 3000000 . C CCG,CG 59.2 PASS TA=1,10;TG=11,2,3,20,0,30;TR=11,2,20;AN=8;AC=2,2 GT:GQ 0/1:245 0/1:245 0/2:245 0/2:245 bcftools-1.2/test/merge.4.a.vcf000066400000000000000000000043351246371514100163370ustar00rootroot00000000000000##fileformat=VCFv4.1 ##INFO= ##FORMAT= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3000000 id1 C CCG 59.2 PASS AN=4;AC=2;TR=1,2;TA=1;TG=1,2,3 GT:GQ:XR:XA:XG 0/1:245:0,1:1:0,1,2 0/1:245:1,2:2:0,1,2 1 3000000 . C CCG 59.2 PASS AN=4;AC=2 GT:GQ:XR:XA:XG 0/1:245:1,2:2:0,1,2 0/1:245:2,3:3:1,2,3 1 3000002 . C CCG 59.2 PASS AN=4;AC=2 GT:GQ 0/1:245 0/1:245 1 3000002 id2 C CCG 59.2 PASS AN=4;AC=2;TR=1,2;TA=1;TG=1,2,3 GT:GL:XR:XA:XG 0/1:245:.:.:. 0/1:245:.:.:. 1 3000002 id3 C CCG 59.2 PASS AN=4;AC=2;TR=1,2;TA=1;TG=1,2,3 GT:GL:XR:XA:XG 0/1:245:.:.:. 0/1:245:. bcftools-1.2/test/merge.4.b.vcf000066400000000000000000000042641246371514100163410ustar00rootroot00000000000000##fileformat=VCFv4.1 ##INFO= ##FORMAT= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT C D 1 3000000 . C A 59.2 PASS AN=4;AC=2 GT:GQ 0/1:245 0/1:245 1 3000000 id1 C A 59.2 PASS AN=4;AC=2;TR=1,2;TA=1;TG=1,2,3 GT:GQ:XR:XG:XA 0/1:245:4,5:3,4,5:5 0/1:245:6,7:6,7,8:7 1 3000002 id3 C A 59.2 PASS AN=4;AC=2;TR=1,2;TA=1;TG=1,2,3 GT:GQ:XR:XG:XA 0/1:245:. 0/1:245:1,2:1,2,3:2 1 3000002 id2 C A 59.2 PASS AN=4;AC=2;TR=1,2;TA=1;TG=1,2,3 GT:GQ:XR:XG:XA 0/1:245:. 0/1:245:.:.:. 1 3000002 . C A 59.2 PASS AN=4;AC=2 GT:GQ 0/1:245 0/1:245 bcftools-1.2/test/merge.4.out000066400000000000000000000052451246371514100161520ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B C D 1 3000000 id1 C CCG,A 59.2 PASS TR=1,2,2;TA=1,1;TG=1,2,3,2,.,3;AN=8;AC=2,2 GT:GQ:XR:XA:XG 0/1:245:0,1,.:1,.:0,1,2,.,.,. 0/1:245:1,2,.:2,.:0,1,2,.,.,. 0/2:245:4,.,5:.,5:3,.,.,4,.,5 0/2:245:6,.,7:.,7:6,.,.,7,.,8 1 3000000 . C CCG,A 59.2 PASS AN=8;AC=2,2 GT:GQ:XR:XA:XG 0/1:245:1,2,.:2,.:0,1,2,.,.,. 0/1:245:2,3,.:3,.:1,2,3,.,.,. 0/2:245:.:.:. 0/2:245:.:.:. 1 3000002 . C CCG,A 59.2 PASS AN=8;AC=2,2 GT:GQ 0/1:245 0/1:245 0/2:245 0/2:245 1 3000002 id2 C CCG,A 59.2 PASS TR=1,2,2;TA=1,1;TG=1,2,3,2,.,3;AN=8;AC=2,2 GT:GL:XR:XA:XG:GQ 0/1:245,245,-1.56481e-39,.,.,.:.:.:.:. 0/1:245,-1.56481e-39,1.09706e-28,.,.,.:.:.:.:. 0/2:.:.:.:.:245 0/2:.:.:.:.:245 1 3000002 id3 C CCG,A 59.2 PASS TR=1,2,2;TA=1,1;TG=1,2,3,2,.,3;AN=8;AC=2,2 GT:GL:XR:XA:XG:GQ 0/1:245,245,-1.56481e-39,.,.,.:.:.:.:. 0/1:245,-1.56481e-39,1.09706e-28,.,.,.:.:.:.:. 0/2:.:.:.:.:245 0/2:.:1,.,2:.,2:1,.,.,2,.,3:245 bcftools-1.2/test/merge.a.chk000066400000000000000000000030111246371514100161520ustar00rootroot00000000000000# This file was produced by vcfcheck and can be plotted using plot-vcfcheck. # # Definition of sets: # ID [2]id [3]tab-separated file names # SN, Summary numbers: # SN [2]id [3]key [4]value SN 0 number of samples: 2 SN 0 number of SNPs: 1 SN 0 number of MNPs: 0 SN 0 number of indels: 10 SN 0 number of others: 0 SN 0 number of multiallelic sites: 4 SN 0 ts/tv: 0.00 # Sis, Singleton stats: # SiS [2]id [3]allele count [4]number of SNPs [5]number of transitions [6]number of transversions [7]number of indels SiS 0 1 2 0 2 0 # AF, Stats by non-reference allele frequency: # AF [2]id [3]allele frequency [4]number of SNPs [5]number of transitions [6]number of transversions [7]number of indels AF 0 0.000000 2 0 2 0 AF 0 49.494949 0 0 0 12 AF 0 100.000000 0 0 0 1 # IDD, InDel distribution: # IDD [2]id [3]length (deletions negative) [4]count IDD 0 -8 1 IDD 0 -4 1 IDD 0 -3 3 IDD 0 -2 2 IDD 0 -1 3 IDD 0 1 2 IDD 0 2 1 # ST, Substitution types: # ST [2]id [3]type [4]count ST 0 A>C 0 ST 0 A>G 0 ST 0 A>T 0 ST 0 C>A 0 ST 0 C>G 0 ST 0 C>T 0 ST 0 G>A 0 ST 0 G>C 1 ST 0 G>T 1 ST 0 T>A 0 ST 0 T>C 0 ST 0 T>G 0 # PSC, Per-sample counts # PSC [2]id [3]sample [4]nRefHom [5]nNonRefHom [6]nHets [7]nTransitions [8]nTransversions [9]nIndels [10]average depth PSC 0 A 0 0 1 0 1 10 27 PSC 0 B 0 0 1 0 1 10 27 # DP, Depth distribution # DP [2]id [3]bin [4]number of genotypes [5]fraction of genotypes (%) DP 0 10 2 9.090909 DP 0 21 2 9.090909 DP 0 22 2 9.090909 DP 0 26 4 18.181818 DP 0 30 2 9.090909 DP 0 31 2 9.090909 DP 0 32 4 18.181818 DP 0 35 4 18.181818 bcftools-1.2/test/merge.a.vcf000066400000000000000000000046731246371514100162020ustar00rootroot00000000000000##fileformat=VCFv4.1 ##INFO= ##FORMAT= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3000150 . C T 59.2 PASS AN=4;AC=2 GT:GQ 0/1:245 0/1:245 1 3000151 . C T 59.2 PASS AN=4;AC=2 GT:DP:GQ 0/1:32:245 0/1:32:245 1 3062915 id3D GTTT G 12.9 q10 DP4=1,2,3,4;AN=4;AC=2;INDEL;STR=test GT:GQ:DP:GL 0/1:409:35:-20,-5,-20 0/1:409:35:-20,-5,-20 1 3062915 idSNP G T,C 12.6 test TEST=5;DP4=1,2,3,4;AN=3;AC=1,1 GT:TT:GQ:DP:GL 0/1:0,1:409:35:-20,-5,-20,-20,-5,-20 2:0,1:409:35:-20,-5,-20 1 3106154 . CAAA C 342 PASS AN=4;AC=2 GT:GQ:DP 0/1:245:32 0/1:245:32 1 3106154 . C CT 59.2 PASS AN=4;AC=2 GT:GQ:DP 0/1:245:32 0/1:245:32 1 3157410 . GA G 90.6 q10 AN=4;AC=4 GT:GQ:DP 1/1:21:21 1/1:21:21 1 3162006 . GAA G 60.2 PASS AN=4;AC=2 GT:GQ:DP 0/1:212:22 0/1:212:22 1 3177144 . G T 45 PASS AN=4;AC=2 GT:GQ:DP 0/0:150:30 1/1:150:30 1 3177144 . G . 45 PASS AN=4;AC=0 GT:GQ:DP 0/0:150:30 0/0:150:30 1 3184885 . TAAAA TA,T 61.5 PASS AN=4;AC=2,2 GT:GQ:DP 1/2:12:10 1/2:12:10 2 3199812 . G GTT,GT 82.7 PASS AN=4;AC=2,2 GT:GQ:DP 1/2:322:26 1/2:322:26 3 3212016 . CTT C,CT 79 PASS AN=4;AC=2,2 GT:GQ:DP 1/2:91:26 1/2:91:26 4 3258448 . TACACACAC T . PASS AN=4;AC=2 GT:GQ:DP 0/1:325:31 0/1:325:31 bcftools-1.2/test/merge.abc.out000066400000000000000000000125611246371514100165330ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##SAMPLE=\") quoting nonsense where double brackets would do just fine",softwareName=,softwareVer=<119,65>,softwareParam=<.>,MetadataResource=http://somewhere.com/path> ##FORMAT= ##FILTER= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 2:B C D 1 3000150 . C T 59.2 PASS AN=4;AC=2 GT:GQ 0/1:245 0/1:245 ./.:. ./.:. ./.:. 1 3000151 . C T 59.2 PASS AN=4;AC=2 GT:DP:GQ 0/1:32:245 0/1:32:245 ./.:.:. ./.:.:. ./.:.:. 1 3062915 id3D GTTT G 84.6 q10;q20 INDEL;STR=test;TXT=AA;DP=1013;DP4=6,7,8,9;AN=10;AC=5 GT:GQ:DP:GL 0/1:409:35:-20,-5,-20 0/1:409:35:-20,-5,-20 0/1:376:14:-10,0,-10 0/1:409:35:-20,-5,-20 0/1:409:35:-20,-5,-20 1 3062915 id1D;id2D GTT GT,G 999 q20;q10 DP=14;DP4=2,4,6,8;AN=6;AC=1,2 GT:GQ:DP:GL:STR ./.:.:.:.:. ./.:.:.:.:. 0/1:376:14:-10,0,-10,.,.,.:DEF 0/2:409:35:-20,.,.,-5,.,-20:. 0/2:409:35:-20,.,.,-5,.,-20:. 1 3062915 idSNP G T,C,A 419 test;q20 TEST=5;STR=.;DP=14;DP4=3,6,9,12;INTA=2,1,.;AN=9;AC=2,2,1 GT:TT:GQ:DP:GL:STR 0/1:0,1,.:409:35:-20,-5,-20,-20,-5,-20,.,.,.,.:. 2:0,1,.:409:35:-20,-5,-20,.:. 0/3:.:376:14:-10,.,.,.,.,.,0,.,.,-10:ABC 0/2:1,0,.:409:35:-20,-20,-20,-5,-5,-20,.,.,.,.:. 0/1:1,0,.:409:35:-20,-20,-20,-5,-5,-20,.,.,.,.:. 1 3106154 . CAAAA CA,C 342 PASS DP=15;AN=6;AC=2,1 GT:GQ:DP:GL 0/1:245:32:. 0/1:245:32:. 0/2:277:15:-10,.,.,0,.,-10 .:245:32:. ./.:245:32:. 1 3106154 . C CT 459 PASS AN=8;AC=4 GT:GQ:DP 0/1:245:32 0/1:245:32 ./.:.:. 0/1:245:32 0/1:245:32 1 3106154 . C T 999 PASS DP=15;AN=2;AC=1 GT:GQ:DP:GL ./.:.:.:. ./.:.:.:. 0/1:277:15:-10,0,-10 ./.:.:.:. ./.:.:.:. 1 3157410 . GAC GC,G 90.6 q10 DP=11;AN=6;AC=4,1 GT:GQ:DP 1/1:21:21 1/1:21:21 0/2:49:11 ./.:.:. ./.:.:. 1 3157410 . G T 46.7 q10 AN=4;AC=4 GT:GQ:DP ./.:.:. ./.:.:. ./.:.:. 1/1:21:21 1/1:21:21 1 3162006 . GAA G,GA 238 PASS DP=19;XRF=1e+06,2e+06,500000;XRI=1111,2222,5555;XRS=AAA,BBB,DDD;XAF=1e+06,500000;XAI=1111,5555;XAS=AAA,DDD;XGF=1e+06,2e+06,3e+06,500000,.,9e+09;XGI=1111,2222,3333,5555,.,9999;XGS=A,B,C,E,.,F;AN=10;AC=3,2 GT:GQ:DP 0/1:212:22 0/1:212:22 0/1:589:19 0/2:212:22 0/2:212:22 1 3177144 . G T 999 PASS DP=24;AN=10;AC=3 GT:GQ:DP 0/0:150:30 1/1:150:30 0/1:236:24 0/0:150:30 0/0:150:30 1 3177144 . G . 45 PASS AN=4 GT:GQ:DP 0/0:150:30 0/0:150:30 ./.:.:. ./.:.:. ./.:.:. 1 3177144 . GT G 999 PASS DP=24;AN=2;AC=1 GT:GQ:DP ./.:.:. ./.:.:. 0/1:236:24 ./.:.:. ./.:.:. 1 3184885 . TAAAA TA,T 61.5 PASS DP=16;AN=10;AC=5,4 GT:GQ:DP 1/2:12:10 1/2:12:10 0/1:435:16 1/2:12:10 1/2:12:10 2 3188209 . GA G 41.5 . DP=15;AN=2;AC=1 GT:GQ:DP ./.:.:. ./.:.:. 0/1:162:15 ./.:.:. ./.:.:. 2 3199812 . G GTT,GT 291 PASS AN=8;AC=4,4 GT:GQ:DP 1/2:322:26 1/2:322:26 ./.:.:. 1/2:322:26 1/2:322:26 3 3199812 . GA GTT,GT 17.5 PASS DP=19;AN=2;AC=1,1 GT:GQ:DP ./.:.:. ./.:.:. 1/2:188:19 ./.:.:. ./.:.:. 3 3212016 . CTT C,CT 79 PASS AN=8;AC=4,4 GT:GQ:DP 1/2:91:26 1/2:91:26 ./.:.:. 1/2:91:26 1/2:91:26 4 3212016 . CTT C 999 q20 DP=15;AN=2;AC=1 GT:GQ:DP ./.:.:. ./.:.:. 0/1:158:15 ./.:.:. ./.:.:. 4 3258448 . TACACACAC T . 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GA G 90.6 q10 . GT:GQ:DP 1/1:21:21 1/1:21:21 1 3162006 . GAA G 60.2 PASS . GT:GQ:DP 0/0:212:22 0/0:212:22 1 3177144 . G T 45 PASS . GT:GQ:DP 0/0:150:30 0/0:150:30 1 3177144 . G . 45 PASS . GT:GQ:DP 0/0:150:30 0/0:150:30 1 3184885 . TAAAA TA,T 61.5 PASS . GT:GQ:DP 0/0:12:10 0/0:12:10 2 3199812 . G GTT,GT 82.7 PASS . GT:GQ:DP 0/0:322:26 0/0:322:26 3 3212016 . CTT C,CT 79 PASS . GT:GQ:DP 0/0:91:26 0/0:91:26 4 3258448 . TACACACAC T 59.9 PASS . 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DP=25;VDB=0.842082;SGB=-4.20907;RPB=0.950652;MQB=1;MQSB=1;BQB=0.929717;MQ0F=0;ICB=0.8;HOB=0.222222;AC=4;AN=6;DP4=2,4,8,11;MQ=60 GT:PL:DP:DV 0/1:211,0,35:12:10 0/1:116,0,91:9:5 1/1:120,12,0:4:4 17 834 . G A 364 . DP=25;VDB=0.788006;SGB=-4.01214;RPB=0.999233;MQB=1;MQSB=1;BQB=0.821668;MQ0F=0;ICB=0.8;HOB=0.222222;AC=4;AN=6;DP4=2,3,7,10;MQ=60 GT:PL:DP:DV 0/1:185,0,46:11:9 0/1:128,0,59:8:5 1/1:89,9,0:3:3 17 1665 . T C 3.10665 . DP=20;VDB=0.1;SGB=0.346553;RPB=0.222222;MQB=0.611111;MQSB=0.988166;BQB=0.944444;MQ0F=0;ICB=0.128205;HOB=0.0555556;AC=1;AN=6;DP4=7,11,1,1;MQ=55 GT:PL:DP:DV 0/0:0,21,185:7:0 0/0:0,27,222:9:0 0/1:35,0,51:4:2 17 1869 . A T 138 . DP=24;VDB=0.928022;SGB=-11.9537;RPB=0.984127;MQB=0.96464;MQSB=0.931547;BQB=0.359155;MQ0F=0;ICB=0.8;HOB=0.222222;AC=4;AN=6;DP4=6,9,5,4;MQ=58 GT:PL:DP:DV 0/1:115,0,224:18:7 0/1:16,0,104:5:1 1/1:42,3,0:1:1 17 2041 . G A 447 . DP=31;VDB=0.816435;SGB=-4.18892;RPB=0.88473;MQB=0.972375;MQSB=0.968257;BQB=0.311275;MQ0F=0;ICB=0.8;HOB=0.222222;AC=4;AN=6;DP4=6,5,12,7;MQ=58 GT:PL:DP:DV 0/1:229,0,212:21:11 0/1:32,0,24:2:1 1/1:223,21,0:7:7 17 2220 . G A 303 . DP=21;VDB=0.532753;SGB=-3.51597;RPB=0.964198;MQB=0.898397;MQSB=0.875769;BQB=0.0354359;MQ0F=0;ICB=0.8;HOB=0.222222;AC=4;AN=6;DP4=6,2,1,11;MQ=58 GT:PL:DP:DV 0/1:139,0,130:12:6 0/1:69,0,46:4:2 1/1:131,12,0:4:4 17 2564 . A G 233 . DP=15;VDB=0.690812;SGB=-3.20711;RPB=0.197899;MQB=1;MQSB=1;BQB=0.965069;MQ0F=0;ICB=0.8;HOB=0.222222;AC=4;AN=6;DP4=1,4,4,5;MQ=60 GT:PL:DP:DV 0/1:88,0,78:6:3 0/1:57,0,56:4:2 1/1:124,12,0:4:4 17 3104 . C T 24.2837 . DP=25;VDB=0.8;SGB=0.346553;RPB=0.717391;MQB=0.956522;MQSB=0.962269;BQB=0.978261;MQ0F=0;ICB=0.128205;HOB=0.0555556;AC=1;AN=6;DP4=8,15,2,0;MQ=58 GT:PL:DP:DV 0/0:0,48,255:16:0 0/0:0,12,144:4:0 0/1:59,0,93:5:2 17 3587 . G A 358 . DP=29;VDB=0.902044;SGB=-3.91326;RPB=0.800999;MQB=1;MQSB=1;BQB=0.156944;MQ0F=0;ICB=0.8;HOB=0.222222;AC=4;AN=6;DP4=4,7,10,6;MQ=60 GT:PL:DP:DV 0/1:161,0,184:14:7 0/1:22,0,118:5:1 1/1:212,24,0:8:8 17 3936 . A G 469 . 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END=1868 GT 0/0 0/0 0/0 17 1869 . A T 138 . DP=24;VDB=0.928022;SGB=-11.9537;RPB=0.984127;MQB=0.96464;MQSB=0.931547;BQB=0.359155;MQ0F=0;ICB=0.8;HOB=0.222222;AC=4;AN=6;DP4=6,9,5,4;MQ=58 GT:PL:DP:DV 0/1:115,0,224:18:7 0/1:16,0,104:5:1 1/1:42,3,0:1:1 17 1870 . C . . . END=2040 GT 0/0 0/0 0/0 17 2041 . G A 447 . DP=31;VDB=0.816435;SGB=-4.18892;RPB=0.88473;MQB=0.972375;MQSB=0.968257;BQB=0.311275;MQ0F=0;ICB=0.8;HOB=0.222222;AC=4;AN=6;DP4=6,5,12,7;MQ=58 GT:PL:DP:DV 0/1:229,0,212:21:11 0/1:32,0,24:2:1 1/1:223,21,0:7:7 17 2042 . G . . . END=2219 GT 0/0 0/0 0/0 17 2220 . G A 303 . DP=21;VDB=0.532753;SGB=-3.51597;RPB=0.964198;MQB=0.898397;MQSB=0.875769;BQB=0.0354359;MQ0F=0;ICB=0.8;HOB=0.222222;AC=4;AN=6;DP4=6,2,1,11;MQ=58 GT:PL:DP:DV 0/1:139,0,130:12:6 0/1:69,0,46:4:2 1/1:131,12,0:4:4 17 2221 . G . . . END=2563 GT 0/0 0/0 0/0 17 2564 . A G 233 . 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DP=11;I16=11,0,0,0,452,18594,0,0,319,9251,0,0,223,4959,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,15,100:5:0 0,9,72:3:0 0,9,72:3:0 17 2 . A 0 . DP=11;I16=11,0,0,0,439,17587,0,0,319,9251,0,0,226,5030,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,15,100:5:0 0,9,72:3:0 0,9,72:3:0 17 3 . G 0 . DP=11;I16=11,0,0,0,431,16971,0,0,319,9251,0,0,229,5111,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,15,100:5:0 0,9,72:3:0 0,9,72:3:0 17 4 . C 0 . DP=11;I16=11,0,0,0,423,16417,0,0,319,9251,0,0,232,5202,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,15,100:5:0 0,9,72:3:0 0,9,71:3:0 17 5 . T 0 . DP=11;I16=11,0,0,0,450,18520,0,0,319,9251,0,0,234,5252,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,15,100:5:0 0,9,72:3:0 0,9,72:3:0 17 6 . T 0 . DP=11;I16=11,0,0,0,403,14847,0,0,319,9251,0,0,236,5310,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,15,100:5:0 0,9,72:3:0 0,9,72:3:0 17 7 . C 0 . DP=11;I16=11,0,0,0,446,18114,0,0,319,9251,0,0,237,5327,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,15,100:5:0 0,9,72:3:0 0,9,72:3:0 17 8 . T 0 . DP=11;I16=11,0,0,0,465,19677,0,0,319,9251,0,0,238,5354,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,15,100:5:0 0,9,72:3:0 0,9,72:3:0 17 9 . C 0 . DP=11;I16=11,0,0,0,447,18205,0,0,319,9251,0,0,239,5391,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,15,100:5:0 0,9,72:3:0 0,9,72:3:0 17 10 . A 0 . DP=11;I16=11,0,0,0,426,16756,0,0,319,9251,0,0,240,5438,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,15,100:5:0 0,9,72:3:0 0,9,69:3:0 17 11 . C 0 . DP=11;I16=11,0,0,0,413,15603,0,0,319,9251,0,0,241,5495,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,15,100:5:0 0,9,72:3:0 0,9,72:3:0 17 12 . C 0 . DP=11;I16=11,0,0,0,438,17506,0,0,319,9251,0,0,242,5562,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,15,100:5:0 0,9,72:3:0 0,9,72:3:0 17 13 . C 0 . DP=11;I16=11,0,0,0,437,17463,0,0,319,9251,0,0,243,5639,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,15,100:5:0 0,9,72:3:0 0,9,72:3:0 17 14 . T 0 . DP=11;I16=11,0,0,0,453,18715,0,0,319,9251,0,0,242,5628,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,15,100:5:0 0,9,72:3:0 0,9,72:3:0 17 15 . G 0 . DP=11;I16=11,0,0,0,439,17599,0,0,319,9251,0,0,240,5580,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,15,100:5:0 0,9,72:3:0 0,9,72:3:0 17 16 . T 0 . DP=11;I16=11,0,0,0,426,16546,0,0,319,9251,0,0,238,5544,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,15,100:5:0 0,9,72:3:0 0,9,72:3:0 17 17 . T 0 . DP=11;I16=11,0,0,0,407,15195,0,0,319,9251,0,0,235,5469,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,15,100:5:0 0,9,72:3:0 0,9,72:3:0 17 18 . C 0 . DP=12;I16=12,0,0,0,450,17136,0,0,379,12851,0,0,231,5353,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,18,113:6:0 0,9,72:3:0 0,9,72:3:0 17 19 . C 0 . DP=13;I16=13,0,0,0,502,19652,0,0,439,16451,0,0,228,5246,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,18,112:6:0 0,12,89:4:0 0,9,72:3:0 17 20 . T 0 . DP=13;I16=13,0,0,0,532,21878,0,0,439,16451,0,0,226,5150,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,18,116:6:0 0,12,98:4:0 0,9,72:3:0 17 21 . G 0 . DP=13;I16=13,0,0,0,498,19254,0,0,439,16451,0,0,224,5066,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,18,117:6:0 0,12,94:4:0 0,9,72:3:0 17 22 . C 0 . DP=13;I16=13,0,0,0,511,20289,0,0,470,19210,0,0,223,4993,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,18,115:6:0 0,12,91:4:0 0,9,76:3:0 17 23 . A 0 . DP=14;I16=14,0,0,0,513,19399,0,0,530,22810,0,0,223,4931,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,133:7:0 0,12,82:4:0 0,9,82:3:0 17 24 . T 0 . DP=14;I16=14,0,0,0,534,20540,0,0,530,22810,0,0,224,4882,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,131:7:0 0,12,96:4:0 0,9,80:3:0 17 25 . A 0 . DP=15;I16=15,0,0,0,561,21133,0,0,590,26410,0,0,225,4847,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,140:8:0 0,12,96:4:0 0,9,81:3:0 17 26 . G 0 . DP=15;I16=15,0,0,0,591,23429,0,0,590,26410,0,0,227,4827,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,151:8:0 0,12,96:4:0 0,9,81:3:0 17 27 . A 0 . DP=15;I16=15,0,0,0,588,23120,0,0,590,26410,0,0,229,4823,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,146:8:0 0,12,98:4:0 0,9,83:3:0 17 28 . T 0 . DP=16;I16=16,0,0,0,605,23001,0,0,650,30010,0,0,231,4835,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,147:8:0 0,12,97:4:0 0,12,101:4:0 17 29 . A 0 . DP=17;I16=17,0,0,0,615,22533,0,0,710,33610,0,0,234,4864,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,146:8:0 0,15,102:5:0 0,12,104:4:0 17 30 . A 0 . DP=17;I16=17,0,0,0,660,25914,0,0,710,33610,0,0,238,4912,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,149:8:0 0,15,115:5:0 0,12,108:4:0 17 31 . T 0 . DP=17;I16=17,0,0,0,656,25392,0,0,710,33610,0,0,242,4980,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,150:8:0 0,15,113:5:0 0,12,105:4:0 17 32 . T 0 . DP=17;I16=17,0,0,0,605,21789,0,0,741,36369,0,0,247,5067,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,143:8:0 0,15,104:5:0 0,12,104:4:0 17 33 . G 0 . DP=17;I16=17,0,0,0,659,25757,0,0,741,36369,0,0,252,5124,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,158:8:0 0,15,118:5:0 0,12,105:4:0 17 34 . C 0 . DP=17;I16=17,0,0,0,658,25704,0,0,741,36369,0,0,257,5203,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,151:8:0 0,15,121:5:0 0,12,106:4:0 17 35 . A 0 . DP=18;I16=18,0,0,0,677,25881,0,0,801,39969,0,0,262,5304,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,152:8:0 0,15,109:5:0 0,15,121:5:0 17 36 . T 0 . DP=18;I16=18,0,0,0,678,25796,0,0,801,39969,0,0,268,5428,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,151:8:0 0,15,114:5:0 0,15,125:5:0 17 37 . G 0 . DP=18;I16=18,0,0,0,685,26291,0,0,801,39969,0,0,274,5576,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,157:8:0 0,15,116:5:0 0,15,126:5:0 17 38 . A 0 . DP=18;I16=18,0,0,0,697,27245,0,0,801,39969,0,0,280,5748,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,152:8:0 0,15,116:5:0 0,15,129:5:0 17 39 . C 0 . DP=16;I16=16,0,0,0,591,22311,0,0,743,38287,0,0,288,5942,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,148:7:0 0,12,94:4:0 0,15,123:5:0 17 40 . A 0 . DP=16;I16=16,0,0,0,630,24896,0,0,743,38287,0,0,295,6107,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,146:7:0 0,12,104:4:0 0,15,127:5:0 17 41 . A 0 . DP=17;I16=17,0,0,0,645,24685,0,0,803,41887,0,0,302,6294,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,151:8:0 0,12,104:4:0 0,15,131:5:0 17 42 . T 0 . DP=17;I16=17,0,0,0,618,23118,0,0,803,41887,0,0,310,6504,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,155:8:0 0,12,82:4:0 0,15,127:5:0 17 43 . T 0 . DP=17;I16=17,0,0,0,631,23689,0,0,803,41887,0,0,318,6738,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,151:8:0 0,12,101:4:0 0,15,129:5:0 17 44 . G 0 . DP=17;I16=17,0,0,0,664,26162,0,0,803,41887,0,0,324,6896,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,164:8:0 0,12,100:4:0 0,15,129:5:0 17 45 . C 0 . DP=17;I16=17,0,0,0,657,25525,0,0,803,41887,0,0,329,7027,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,163:8:0 0,12,108:4:0 0,15,125:5:0 17 46 . C 0 . DP=17;I16=17,0,0,0,646,25244,0,0,803,41887,0,0,334,7180,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,161:8:0 0,12,98:4:0 0,15,131:5:0 17 47 . T 0 . DP=17;I16=17,0,0,0,700,28998,0,0,803,41887,0,0,339,7355,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,165:8:0 0,12,110:4:0 0,15,136:5:0 17 48 . T 0 . DP=17;I16=17,0,0,0,648,25020,0,0,803,41887,0,0,343,7501,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,155:8:0 0,12,105:4:0 0,15,131:5:0 17 49 . G 0 . DP=18;I16=18,0,0,0,686,26674,0,0,832,42728,0,0,346,7616,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,158:8:0 0,15,119:5:0 0,15,128:5:0 17 50 . T 0 . DP=18;I16=17,0,0,0,650,24972,0,0,772,39128,0,0,332,7426,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,155:8:0 0,12,101:4:0 0,15,128:5:0 17 51 . C 0 . DP=18;I16=18,0,0,0,681,26529,0,0,832,42728,0,0,353,7853,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,167:8:0 0,15,99:5:0 0,15,127:5:0 17 52 . C 0 . DP=18;I16=17,0,0,0,645,24983,0,0,803,41887,0,0,353,7965,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,164:8:0 0,12,99:4:0 0,15,128:5:0 17 53 . C 0 . DP=18;I16=18,0,0,0,687,26735,0,0,832,42728,0,0,359,8113,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,164:8:0 0,15,113:5:0 0,15,132:5:0 17 54 . T 0 . DP=18;I16=18,0,0,0,736,30194,0,0,832,42728,0,0,361,8219,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,163:8:0 0,15,128:5:0 0,15,136:5:0 17 55 . G 0 . DP=18;I16=18,0,0,0,674,26082,0,0,832,42728,0,0,362,8290,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,168:8:0 0,15,112:5:0 0,15,122:5:0 17 56 . C 0 . DP=18;I16=18,0,0,0,701,27645,0,0,832,42728,0,0,363,8375,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,162:8:0 0,15,121:5:0 0,15,131:5:0 17 57 . T 0 . DP=18;I16=17,0,0,0,694,29118,0,0,803,41887,0,0,356,8410,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,163:8:0 0,12,117:4:0 0,15,138:5:0 17 58 . G 0 . DP=17;I16=16,0,0,0,616,24356,0,0,774,41046,0,0,356,8454,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,159:7:0 0,12,95:4:0 0,15,131:5:0 17 59 . A 0 . DP=17;I16=17,0,0,0,656,26038,0,0,803,41887,0,0,366,8606,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,155:7:0 0,15,116:5:0 0,15,134:5:0 17 60 . A 0 . DP=17;I16=17,0,0,0,663,26463,0,0,803,41887,0,0,367,8687,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,151:7:0 0,15,127:5:0 0,15,138:5:0 17 61 . T 0 . DP=17;I16=16,0,0,0,605,23215,0,0,774,41046,0,0,355,8583,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,152:7:0 0,12,101:4:0 0,15,131:5:0 17 62 . G 0 . DP=18;I16=17,0,0,0,646,24868,0,0,834,44646,0,0,353,8557,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,158:7:0 0,12,103:4:0 0,18,141:6:0 17 63 . T 0 . DP=18;I16=17,0,0,0,590,21682,0,0,803,41887,0,0,341,8111,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,148:7:0 0,12,80:4:0 0,18,144:6:0 17 64 . G 0 . DP=19;I16=19,0,0,0,696,26416,0,0,923,49087,0,0,366,8758,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,169:8:0 0,15,103:5:0 0,18,142:6:0 17 65 . C 0 . DP=18;I16=18,0,0,0,686,26512,0,0,894,48246,0,0,367,8743,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,163:7:0 0,15,116:5:0 0,18,147:6:0 17 66 . T 0 . DP=18;I16=17,0,0,0,691,28315,0,0,834,44646,0,0,342,8068,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,164:7:0 0,12,110:4:0 0,18,153:6:0 17 67 . C 0 . DP=17;I16=17,0,0,0,645,25313,0,0,834,44646,0,0,341,7983,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,163:7:0 0,12,94:4:0 0,18,147:6:0 17 68 . T 0 . DP=17;I16=17,0,0,0,691,28307,0,0,834,44646,0,0,339,7863,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,165:7:0 0,12,108:4:0 0,18,151:6:0 17 69 . G 0 . DP=17;I16=17,0,0,0,657,25721,0,0,865,47405,0,0,338,7758,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,172:8:0 0,9,93:3:0 0,18,142:6:0 17 70 . G 0 . DP=17;I16=16,0,0,0,575,21391,0,0,836,46564,0,0,317,7227,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,162:8:0 0,6,64:2:0 0,18,141:6:0 17 71 . G 0 . DP=17;I16=15,0,0,0,571,21975,0,0,776,42964,0,0,307,7029,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,170:8:0 0,6,72:2:0 0,15,130:5:0 17 72 . G 0 . DP=17;I16=15,0,0,0,572,22002,0,0,776,42964,0,0,305,6907,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,171:8:0 0,6,65:2:0 0,15,131:5:0 17 73 . T 0 . DP=18;I16=16,0,0,0,623,25301,0,0,836,46564,0,0,314,6918,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,158:8:0 0,6,94:2:0 0,18,143:6:0 17 74 . C 0 . DP=18;I16=17,0,0,0,674,28110,0,0,865,47405,0,0,338,7468,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,176:8:0 0,9,106:3:0 0,18,141:6:0 17 75 . T 0 . DP=18;I16=16,0,0,0,685,30675,0,0,836,46564,0,0,312,6782,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,175:8:0 0,6,90:2:0 0,18,150:6:0 17 76 . C 0 . DP=18;I16=17,0,0,0,683,29481,0,0,865,47405,0,0,336,7360,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,174:8:0 0,9,98:3:0 0,18,148:6:0 17 77 . T 0 . DP=18;I16=16,0,0,0,710,33072,0,0,836,46564,0,0,310,6702,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,179:8:0 0,6,92:2:0 0,18,154:6:0 17 78 . G 0 . DP=18;I16=16,0,0,0,692,31158,0,0,836,46564,0,0,309,6683,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,180:8:0 0,6,94:2:0 0,18,149:6:0 17 79 . G 0 . DP=18;I16=15,0,0,0,605,26629,0,0,776,42964,0,0,283,6053,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,168:8:0 0,3,60:1:0 0,18,149:6:0 17 80 . G 0 . DP=18;I16=17,0,0,0,688,30128,0,0,865,47405,0,0,332,7312,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,173:8:0 0,9,106:3:0 0,18,151:6:0 17 81 . G 0 . DP=18;I16=16,0,0,0,631,27429,0,0,836,46564,0,0,326,7310,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,165:7:0 0,9,97:3:0 0,18,145:6:0 17 82 . T 0 . DP=18;I16=15,0,0,0,559,22735,0,0,776,42964,0,0,280,6122,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,154:8:0 0,3,60:1:0 0,18,131:6:0 17 83 . C 0 . DP=18;I16=16,0,0,0,622,27146,0,0,836,46564,0,0,304,6798,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,170:8:0 0,6,75:2:0 0,18,145:6:0 17 84 . T 0 . DP=18;I16=17,0,0,0,705,32445,0,0,865,47405,0,0,328,7488,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,181:8:0 0,9,85:3:0 0,18,154:6:0 17 85 . C 0 . DP=18;I16=17,0,0,0,708,31222,0,0,865,47405,0,0,327,7567,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,181:8:0 0,9,108:3:0 0,18,153:6:0 17 86 . A 0 . DP=18;I16=17,0,0,0,692,29540,0,0,865,47405,0,0,326,7660,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,169:8:0 0,9,111:3:0 0,18,149:6:0 17 87 . C 0 . DP=18;I16=17,0,0,0,683,29493,0,0,896,50164,0,0,326,7766,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,174:8:0 0,9,103:3:0 0,18,148:6:0 17 88 . C 0 . DP=18;I16=17,0,0,0,703,31005,0,0,896,50164,0,0,326,7834,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,174:8:0 0,9,113:3:0 0,18,153:6:0 17 89 . C 0 . DP=18;I16=17,0,0,0,697,30875,0,0,896,50164,0,0,326,7914,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,176:8:0 0,9,103:3:0 0,18,154:6:0 17 90 . A 0 . DP=17;I16=16,0,0,0,668,29732,0,0,867,49323,0,0,326,7954,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,173:8:0 0,9,114:3:0 0,15,138:5:0 17 91 . C 0 . DP=16;I16=15,0,0,0,613,26409,0,0,838,48482,0,0,327,8001,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,170:7:0 0,9,113:3:0 0,15,133:5:0 17 92 . G 0 . DP=16;I16=15,0,0,0,499,18731,0,0,838,48482,0,0,328,8054,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,143:7:0 0,9,100:3:0 0,15,96:5:0 17 93 . A 0 . DP=16;I16=15,0,0,0,635,28655,0,0,869,51241,0,0,329,8063,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,167:7:0 0,9,108:3:0 0,15,145:5:0 17 94 . C 0 . DP=16;I16=15,0,0,0,607,25893,0,0,869,51241,0,0,331,8079,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,21,166:7:0 0,9,105:3:0 0,15,135:5:0 17 95 . C 0 . DP=17;I16=15,0,0,0,615,26761,0,0,900,54000,0,0,306,7378,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,178:8:0 0,6,91:2:0 0,15,135:5:0 17 96 . A 0 . DP=17;I16=16,0,0,0,625,26705,0,0,929,54841,0,0,332,7936,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,172:8:0 0,9,99:3:0 0,15,134:5:0 17 97 . A 0 . DP=18;I16=17,0,0,0,675,29017,0,0,958,55682,0,0,333,7879,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,27,179:9:0 0,9,104:3:0 0,15,145:5:0 17 98 . C 0 . DP=18;I16=17,0,0,0,655,27231,0,0,958,55682,0,0,333,7735,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,27,179:9:0 0,9,105:3:0 0,15,131:5:0 17 99 . T 0 . DP=18;I16=17,0,0,0,720,32840,0,0,958,55682,0,0,333,7607,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,27,186:9:0 0,9,115:3:0 0,15,153:5:0 17 100 . C 0 . DP=18;I16=17,0,0,0,688,29762,0,0,958,55682,0,0,332,7446,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,27,189:9:0 0,9,108:3:0 0,15,134:5:0 17 101 . C 0 . DP=18;I16=17,0,0,0,650,27530,0,0,958,55682,0,0,331,7303,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,27,182:9:0 0,9,99:3:0 0,15,132:5:0 17 102 . C 0 . DP=18;I16=17,0,0,0,695,30453,0,0,958,55682,0,0,330,7178,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,27,188:9:0 0,9,111:3:0 0,15,139:5:0 17 103 . T 0 . DP=18;I16=16,0,0,0,692,31998,0,0,929,54841,0,0,323,7035,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,189:8:0 0,9,108:3:0 0,15,147:5:0 17 104 . G 0 . DP=18;I16=15,0,0,0,611,26723,0,0,900,54000,0,0,295,6259,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,24,178:8:0 0,6,89:2:0 0,15,133:5:0 17 105 . G 0 . DP=19;I16=17,0,0,0,604,23936,0,0,989,58441,0,0,317,6751,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,27,170:9:0 0,9,97:3:0 0,15,125:5:0 17 106 . G 0 . DP=19;I16=17,0,0,0,644,26574,0,0,989,58441,0,0,299,6093,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,30,190:10:0 0,6,85:2:0 0,15,124:5:0 17 107 . C 0 . DP=19;I16=17,0,0,0,694,30064,0,0,989,58441,0,0,313,6543,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,27,192:9:0 0,9,108:3:0 0,15,136:5:0 17 108 . C 0 . DP=19;I16=17,0,0,0,692,30148,0,0,989,58441,0,0,310,6420,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,27,190:9:0 0,9,108:3:0 0,15,135:5:0 17 109 . T 0 . DP=19;I16=17,0,0,0,741,34273,0,0,989,58441,0,0,307,6319,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,27,195:9:0 0,9,110:3:0 0,15,150:5:0 17 110 . G 0 . DP=19;I16=17,0,0,0,704,31276,0,0,989,58441,0,0,304,6240,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,27,194:9:0 0,9,104:3:0 0,15,136:5:0 17 111 . G 0 . DP=19;I16=16,0,0,0,584,24362,0,0,929,54841,0,0,272,5416,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,30,167:10:0 0,6,88:2:0 0,12,118:4:0 17 112 . C 0 . DP=19;I16=17,0,0,0,680,29854,0,0,989,58441,0,0,296,6052,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,27,191:9:0 0,9,95:3:0 0,15,135:5:0 17 113 . A 0 . DP=19;I16=16,0,0,0,645,28035,0,0,960,57600,0,0,266,5318,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,27,176:9:0 0,6,87:2:0 0,15,139:5:0 17 114 . C 0 . DP=19;I16=17,0,0,0,674,28788,0,0,989,58441,0,0,286,5856,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,27,182:9:0 0,9,103:3:0 0,15,133:5:0 17 115 . C 0 . DP=21;I16=18,0,0,0,708,30546,0,0,1049,62041,0,0,274,5490,0,0;QS=3,0;MQ0F=0 PL:DP:DV 0,30,189:10:0 0,6,89:2:0 0,18,147:6:0 17 116 . A 0 . DP=21;I16=17,1,0,0,727,31755,0,0,1049,62041,0,0,253,5079,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,183:9:0 0,6,90:2:0 0,21,175:7:0 17 117 . G 0 . DP=21;I16=17,1,0,0,723,31221,0,0,1049,62041,0,0,249,5019,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,186:9:0 0,6,86:2:0 0,21,177:7:0 17 118 . G 0 . DP=20;I16=16,1,0,0,636,26574,0,0,958,55682,0,0,266,5426,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,175:9:0 0,3,60:1:0 0,21,162:7:0 17 119 . G 0 . DP=19;I16=16,1,0,0,629,26439,0,0,958,55682,0,0,267,5553,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,175:8:0 0,6,73:2:0 0,21,160:7:0 17 120 . A 0 . DP=19;I16=16,1,0,0,672,29188,0,0,958,55682,0,0,264,5518,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,175:8:0 0,6,83:2:0 0,21,171:7:0 17 121 . G 0 . DP=19;I16=16,1,0,0,662,28460,0,0,958,55682,0,0,260,5454,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,181:8:0 0,6,80:2:0 0,21,168:7:0 17 122 . C 0 . DP=20;I16=17,1,0,0,716,31224,0,0,1018,59282,0,0,256,5410,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,181:8:0 0,9,99:3:0 0,21,178:7:0 17 123 . T 0 . DP=18;I16=15,1,0,0,661,29997,0,0,898,52082,0,0,255,5385,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,167:7:0 0,9,112:3:0 0,18,166:6:0 17 124 . T 0 . DP=19;I16=17,1,0,0,626,24802,0,0,987,56523,0,0,279,6003,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,154:9:0 0,9,104:3:0 0,18,154:6:0 17 125 . A 0 . DP=18;I16=15,1,0,0,611,25689,0,0,898,52082,0,0,254,5340,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,154:7:0 0,9,104:3:0 0,18,162:6:0 17 126 . A 0 . DP=18;I16=16,1,0,0,648,27366,0,0,927,52923,0,0,279,5947,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,162:8:0 0,9,107:3:0 0,18,174:6:0 17 127 . C 0 . DP=18;I16=16,1,0,0,646,26972,0,0,927,52923,0,0,279,5949,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,163:8:0 0,9,109:3:0 0,18,160:6:0 17 128 . A 0 . DP=18;I16=16,1,0,0,673,28797,0,0,927,52923,0,0,279,5971,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,169:8:0 0,9,111:3:0 0,18,162:6:0 17 129 . A 0 . DP=17;I16=15,1,0,0,645,27891,0,0,867,49323,0,0,280,6012,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,168:8:0 0,9,113:3:0 0,15,159:5:0 17 130 . A 0 . DP=17;I16=15,1,0,0,641,27295,0,0,867,49323,0,0,281,6071,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,169:8:0 0,9,113:3:0 0,15,152:5:0 17 131 . C 0 . DP=16;I16=14,1,0,0,606,25732,0,0,838,48482,0,0,256,5472,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,167:7:0 0,9,110:3:0 0,15,147:5:0 17 132 . A 0 . DP=16;I16=14,1,0,0,627,27579,0,0,838,48482,0,0,256,5514,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,169:7:0 0,9,110:3:0 0,15,151:5:0 17 133 . T 0 . DP=15;I16=13,2,0,0,584,22816,0,0,838,48482,0,0,282,6196,0,0;QS=3,0;MQSB=0.576923;MQ0F=0 PL:DP:DV 0,21,163:7:0 0,9,105:3:0 0,15,150:5:0 17 134 . C 0 . DP=15;I16=13,2,0,0,607,24653,0,0,838,48482,0,0,283,6267,0,0;QS=3,0;MQSB=0.576923;MQ0F=0 PL:DP:DV 0,21,177:7:0 0,9,105:3:0 0,15,152:5:0 17 135 . T 0 . DP=15;I16=13,2,0,0,600,24178,0,0,838,48482,0,0,284,6352,0,0;QS=3,0;MQSB=0.576923;MQ0F=0 PL:DP:DV 0,21,173:7:0 0,9,106:3:0 0,15,156:5:0 17 136 . G 0 . DP=15;I16=13,2,0,0,574,22258,0,0,838,48482,0,0,286,6450,0,0;QS=3,0;MQSB=0.576923;MQ0F=0 PL:DP:DV 0,21,172:7:0 0,9,105:3:0 0,15,134:5:0 17 137 . T 0 . DP=15;I16=13,2,0,0,563,21377,0,0,838,48482,0,0,289,6561,0,0;QS=3,0;MQSB=0.576923;MQ0F=0 PL:DP:DV 0,21,160:7:0 0,9,104:3:0 0,15,139:5:0 17 138 . C 0 . DP=15;I16=13,2,0,0,584,23088,0,0,838,48482,0,0,291,6637,0,0;QS=3,0;MQSB=0.576923;MQ0F=0 PL:DP:DV 0,21,172:7:0 0,9,108:3:0 0,15,142:5:0 17 139 . C 0 . DP=15;I16=13,2,0,0,554,20790,0,0,838,48482,0,0,292,6680,0,0;QS=3,0;MQSB=0.576923;MQ0F=0 PL:DP:DV 0,21,161:7:0 0,9,106:3:0 0,15,143:5:0 17 140 . A 0 . DP=15;I16=13,2,0,0,583,22789,0,0,838,48482,0,0,292,6690,0,0;QS=3,0;MQSB=0.576923;MQ0F=0 PL:DP:DV 0,21,163:7:0 0,9,107:3:0 0,15,153:5:0 17 141 . G 0 . DP=14;I16=12,2,0,0,534,20750,0,0,778,44882,0,0,292,6664,0,0;QS=3,0;MQSB=0.583333;MQ0F=0 PL:DP:DV 0,18,158:6:0 0,9,108:3:0 0,15,142:5:0 17 142 . C 0 . DP=14;I16=12,2,0,0,503,18593,0,0,778,44882,0,0,292,6650,0,0;QS=3,0;MQSB=0.583333;MQ0F=0 PL:DP:DV 0,18,157:6:0 0,9,97:3:0 0,15,129:5:0 17 143 . G 0 . DP=14;I16=11,2,0,0,415,13657,0,0,718,41282,0,0,285,6599,0,0;QS=3,0;MQSB=0.590909;MQ0F=0 PL:DP:DV 0,18,128:6:0 0,9,95:3:0 0,12,97:4:0 17 144 . A 0 . DP=14;I16=12,2,0,0,519,19725,0,0,778,44882,0,0,291,6609,0,0;QS=3,0;MQSB=0.583333;MQ0F=0 PL:DP:DV 0,18,152:6:0 0,9,105:3:0 0,15,129:5:0 17 145 . A 0 . DP=14;I16=12,2,0,0,527,20289,0,0,778,44882,0,0,290,6584,0,0;QS=3,0;MQSB=0.583333;MQ0F=0 PL:DP:DV 0,18,153:6:0 0,9,106:3:0 0,15,138:5:0 17 146 . T 0 . DP=14;I16=12,2,0,0,514,19484,0,0,778,44882,0,0,289,6573,0,0;QS=3,0;MQSB=0.583333;MQ0F=0 PL:DP:DV 0,18,152:6:0 0,9,103:3:0 0,15,128:5:0 17 147 . A 0 . DP=14;I16=12,2,0,0,515,19213,0,0,778,44882,0,0,288,6576,0,0;QS=3,0;MQSB=0.583333;MQ0F=0 PL:DP:DV 0,18,150:6:0 0,9,99:3:0 0,15,140:5:0 17 148 . C 0 . DP=14;I16=12,2,0,0,541,21019,0,0,778,44882,0,0,286,6542,0,0;QS=3,0;MQSB=0.583333;MQ0F=0 PL:DP:DV 0,18,157:6:0 0,9,106:3:0 0,15,146:5:0 17 149 . C 0 . DP=14;I16=12,2,0,0,512,19326,0,0,778,44882,0,0,283,6471,0,0;QS=3,0;MQSB=0.583333;MQ0F=0 PL:DP:DV 0,18,148:6:0 0,9,109:3:0 0,15,140:5:0 17 150 . T 0 . DP=13;I16=11,2,0,0,511,20251,0,0,749,44041,0,0,280,6362,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,18,153:6:0 0,6,84:2:0 0,15,152:5:0 17 151 . G 0 . DP=13;I16=11,2,0,0,506,19826,0,0,749,44041,0,0,277,6263,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,18,157:6:0 0,6,84:2:0 0,15,144:5:0 17 152 . C 0 . DP=14;I16=12,2,0,0,543,21283,0,0,809,47641,0,0,274,6174,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,21,168:7:0 0,6,84:2:0 0,15,146:5:0 17 153 . A 0 . DP=14;I16=12,2,0,0,536,20594,0,0,809,47641,0,0,272,6096,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,21,156:7:0 0,6,81:2:0 0,15,153:5:0 17 154 . T 0 . DP=14;I16=12,2,0,0,523,20051,0,0,809,47641,0,0,270,6030,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,21,159:7:0 0,6,83:2:0 0,15,139:5:0 17 155 . C 0 . DP=14;I16=12,2,0,0,542,21254,0,0,809,47641,0,0,268,5976,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,21,172:7:0 0,6,85:2:0 0,15,139:5:0 17 156 . C 0 . DP=14;I16=12,2,0,0,536,20884,0,0,809,47641,0,0,266,5934,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,21,163:7:0 0,6,84:2:0 0,15,150:5:0 17 157 . C 0 . DP=14;I16=12,2,0,0,555,22081,0,0,809,47641,0,0,264,5904,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,21,169:7:0 0,6,85:2:0 0,15,149:5:0 17 158 . T 0 . DP=14;I16=12,2,0,0,568,23154,0,0,809,47641,0,0,262,5886,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,21,170:7:0 0,6,84:2:0 0,15,159:5:0 17 159 . A 0 . DP=15;I16=12,2,0,0,519,19467,0,0,809,47641,0,0,260,5880,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,21,157:7:0 0,6,83:2:0 0,15,135:5:0 17 160 . G 0 . DP=15;I16=13,2,0,0,547,20633,0,0,869,51241,0,0,259,5887,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,21,165:7:0 0,6,85:2:0 0,18,139:6:0 17 161 . A 0 . DP=15;I16=13,2,0,0,568,21610,0,0,869,51241,0,0,258,5908,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,21,157:7:0 0,6,83:2:0 0,18,162:6:0 17 162 . A 0 . DP=15;I16=13,2,0,0,557,21139,0,0,869,51241,0,0,255,5843,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,21,147:7:0 0,6,87:2:0 0,18,167:6:0 17 163 . G 0 . DP=14;I16=12,2,0,0,503,18645,0,0,809,47641,0,0,253,5791,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,21,153:7:0 0,6,79:2:0 0,15,138:5:0 17 164 . T 0 . DP=14;I16=12,2,0,0,460,15968,0,0,809,47641,0,0,252,5750,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,18,131:6:0 0,6,79:2:0 0,18,136:6:0 17 165 . G 0 . DP=14;I16=10,2,0,0,456,17460,0,0,689,40441,0,0,226,5094,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,18,149:6:0 0,6,80:2:0 0,12,122:4:0 17 166 . A 0 . DP=14;I16=11,2,0,0,496,19138,0,0,749,44041,0,0,227,5077,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,18,145:6:0 0,6,82:2:0 0,15,148:5:0 17 167 . A 0 . DP=14;I16=11,2,0,0,477,17851,0,0,749,44041,0,0,227,5071,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,18,132:6:0 0,6,86:2:0 0,15,147:5:0 17 168 . G 0 . DP=14;I16=12,2,0,0,481,18015,0,0,809,47641,0,0,252,5702,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,18,145:6:0 0,6,82:2:0 0,18,140:6:0 17 169 . C 0 . DP=13;I16=10,2,0,0,402,14224,0,0,689,40441,0,0,227,5045,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,15,106:5:0 0,6,76:2:0 0,15,145:5:0 17 170 . C 0 . DP=13;I16=11,2,0,0,447,16383,0,0,749,44041,0,0,251,5601,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,15,128:5:0 0,6,80:2:0 0,18,143:6:0 17 171 . A 0 . DP=13;I16=11,2,0,0,500,19366,0,0,749,44041,0,0,250,5546,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,15,134:5:0 0,6,81:2:0 0,18,166:6:0 17 172 . C 0 . DP=13;I16=10,2,0,0,439,16395,0,0,689,40441,0,0,241,5441,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,15,138:5:0 0,6,75:2:0 0,15,129:5:0 17 173 . C 0 . DP=13;I16=11,2,0,0,435,15225,0,0,749,44041,0,0,248,5478,0,0;QS=3,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,15,121:5:0 0,6,76:2:0 0,18,146:6:0 17 174 . G 0 . DP=13;I16=11,1,0,0,351,10685,0,0,689,40441,0,0,238,5364,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,15,111:5:0 0,3,27:1:0 0,18,117:6:0 17 175 . C 0 . DP=14;I16=13,1,0,0,511,19161,0,0,809,47641,0,0,249,5463,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,143:6:0 0,3,41:1:0 0,21,175:7:0 17 176 . C 0 . DP=14;I16=13,1,0,0,489,17733,0,0,809,47641,0,0,251,5477,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,146:6:0 0,3,44:1:0 0,21,152:7:0 17 177 . C 0 . DP=14;I16=13,1,0,0,488,17328,0,0,809,47641,0,0,253,5507,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,138:6:0 0,3,44:1:0 0,21,158:7:0 17 178 . A 0 . DP=14;I16=13,1,0,0,519,19485,0,0,809,47641,0,0,254,5502,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,147:6:0 0,3,42:1:0 0,21,172:7:0 17 179 . A 0 . DP=14;I16=13,1,0,0,478,17278,0,0,809,47641,0,0,255,5511,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,134:6:0 0,3,44:1:0 0,21,170:7:0 17 180 . A 0 . DP=14;I16=12,1,0,0,425,14653,0,0,749,44041,0,0,250,5498,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,126:6:0 0,3,43:1:0 0,18,148:6:0 17 181 . G 0 . DP=14;I16=11,1,0,0,450,17152,0,0,689,40441,0,0,233,5233,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,156:6:0 0,3,41:1:0 0,15,138:5:0 17 182 . A 0 . DP=15;I16=14,1,0,0,515,18235,0,0,869,51241,0,0,258,5622,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,150:7:0 0,3,43:1:0 0,21,159:7:0 17 183 . C 0 . DP=15;I16=13,1,0,0,483,17419,0,0,809,47641,0,0,235,5063,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,159:7:0 0,3,40:1:0 0,18,139:6:0 17 184 . A 0 . DP=15;I16=14,1,0,0,535,19667,0,0,869,51241,0,0,262,5770,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,158:7:0 0,3,41:1:0 0,21,163:7:0 17 185 . C 0 . DP=15;I16=13,1,0,0,487,17295,0,0,809,47641,0,0,238,5192,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,150:7:0 0,3,38:1:0 0,18,160:6:0 17 186 . G 0 . DP=15;I16=12,1,0,0,381,11429,0,0,749,44041,0,0,239,5253,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,117:6:0 0,3,32:1:0 0,18,124:6:0 17 187 . C 0 . DP=14;I16=13,1,0,0,511,18979,0,0,809,47641,0,0,266,5952,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,147:6:0 0,3,38:1:0 0,21,172:7:0 17 188 . C 0 . DP=14;I16=13,1,0,0,496,18042,0,0,809,47641,0,0,267,5989,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,147:6:0 0,3,37:1:0 0,21,162:7:0 17 189 . C 0 . DP=15;I16=13,2,0,0,552,20504,0,0,838,48482,0,0,268,6040,0,0;QS=3,0;MQSB=0;MQ0F=0 PL:DP:DV 0,18,152:6:0 0,6,67:2:0 0,21,167:7:0 17 190 . A 0 . DP=15;I16=12,2,0,0,500,18230,0,0,778,44882,0,0,243,5381,0,0;QS=3,0;MQSB=0;MQ0F=0 PL:DP:DV 0,18,138:6:0 0,6,68:2:0 0,18,159:6:0 17 191 . T 0 . DP=15;I16=13,2,0,0,534,19276,0,0,838,48482,0,0,267,5939,0,0;QS=3,0;MQSB=0;MQ0F=0 PL:DP:DV 0,18,143:6:0 0,6,67:2:0 0,21,169:7:0 17 192 . G 0 . DP=15;I16=13,2,0,0,499,17439,0,0,838,48482,0,0,266,5890,0,0;QS=3,0;MQSB=0;MQ0F=0 PL:DP:DV 0,18,143:6:0 0,6,67:2:0 0,21,151:7:0 17 193 . T 0 . DP=15;I16=13,2,0,0,505,17811,0,0,838,48482,0,0,265,5859,0,0;QS=3,0;MQSB=0;MQ0F=0 PL:DP:DV 0,18,140:6:0 0,6,63:2:0 0,21,157:7:0 17 194 . C 0 . DP=14;I16=12,2,0,0,467,16569,0,0,778,44882,0,0,265,5845,0,0;QS=3,0;MQSB=0;MQ0F=0 PL:DP:DV 0,18,142:6:0 0,6,67:2:0 0,18,145:6:0 17 195 . C 0 . DP=14;I16=11,3,0,0,503,18647,0,0,747,42123,0,0,266,5846,0,0;QS=3,0;MQSB=0.0368832;MQ0F=0 PL:DP:DV 0,18,159:6:0 0,6,71:2:0 0,18,160:6:0 17 196 . A 0 . DP=14;I16=11,3,0,0,482,17400,0,0,747,42123,0,0,268,5862,0,0;QS=3,0;MQSB=0.0368832;MQ0F=0 PL:DP:DV 0,18,166:6:0 0,6,69:2:0 0,18,138:6:0 17 197 . G 0 . DP=14;I16=11,3,0,0,481,17391,0,0,747,42123,0,0,270,5894,0,0;QS=3,0;MQSB=0.0368832;MQ0F=0 PL:DP:DV 0,18,164:6:0 0,6,68:2:0 0,18,134:6:0 17 198 . C 0 . DP=14;I16=11,3,0,0,539,20957,0,0,747,42123,0,0,271,5893,0,0;QS=3,0;MQSB=0.0368832;MQ0F=0 PL:DP:DV 0,18,172:6:0 0,6,70:2:0 0,18,164:6:0 17 199 . T 0 . DP=14;I16=11,3,0,0,505,19197,0,0,747,42123,0,0,271,5861,0,0;QS=3,0;MQSB=0.0368832;MQ0F=0 PL:DP:DV 0,18,162:6:0 0,6,73:2:0 0,18,154:6:0 17 200 . T 0 . DP=15;I16=11,4,0,0,544,19918,0,0,776,42964,0,0,270,5798,0,0;QS=3,0;MQSB=0.0161635;MQ0F=0 PL:DP:DV 0,18,161:6:0 0,9,89:3:0 0,18,154:6:0 17 201 . A 0 . DP=16;I16=12,4,0,0,568,20416,0,0,836,46564,0,0,269,5703,0,0;QS=3,0;MQSB=0.0144756;MQ0F=0 PL:DP:DV 0,21,171:7:0 0,9,89:3:0 0,18,157:6:0 17 202 . A 0 . DP=16;I16=12,4,0,0,566,20590,0,0,836,46564,0,0,269,5627,0,0;QS=3,0;MQSB=0.0144756;MQ0F=0 PL:DP:DV 0,21,178:7:0 0,9,84:3:0 0,18,163:6:0 17 203 . C 0 . DP=16;I16=12,4,0,0,557,20119,0,0,836,46564,0,0,269,5571,0,0;QS=3,0;MQSB=0.0144756;MQ0F=0 PL:DP:DV 0,21,166:7:0 0,9,90:3:0 0,18,153:6:0 17 204 . C 0 . DP=16;I16=12,4,0,0,591,22379,0,0,836,46564,0,0,269,5535,0,0;QS=3,0;MQSB=0.0144756;MQ0F=0 PL:DP:DV 0,21,173:7:0 0,9,91:3:0 0,18,163:6:0 17 205 . T 0 . DP=16;I16=12,4,0,0,635,25281,0,0,836,46564,0,0,269,5519,0,0;QS=3,0;MQSB=0.0144756;MQ0F=0 PL:DP:DV 0,21,188:7:0 0,9,95:3:0 0,18,173:6:0 17 206 . G 0 . DP=16;I16=12,4,0,0,577,21337,0,0,836,46564,0,0,269,5523,0,0;QS=3,0;MQSB=0.0144756;MQ0F=0 PL:DP:DV 0,21,180:7:0 0,9,89:3:0 0,18,143:6:0 17 207 . C 0 . DP=16;I16=12,4,0,0,574,21076,0,0,836,46564,0,0,269,5547,0,0;QS=3,0;MQSB=0.0144756;MQ0F=0 PL:DP:DV 0,21,179:7:0 0,9,93:3:0 0,18,151:6:0 17 208 . A 0 . DP=16;I16=12,4,0,0,576,21486,0,0,836,46564,0,0,268,5540,0,0;QS=3,0;MQSB=0.0144756;MQ0F=0 PL:DP:DV 0,21,184:7:0 0,9,93:3:0 0,18,154:6:0 17 209 . T 0 . DP=16;I16=12,4,0,0,567,20475,0,0,836,46564,0,0,267,5551,0,0;QS=3,0;MQSB=0.0144756;MQ0F=0 PL:DP:DV 0,21,173:7:0 0,9,91:3:0 0,18,146:6:0 17 210 . C 0 . DP=16;I16=12,4,0,0,577,21109,0,0,836,46564,0,0,266,5580,0,0;QS=3,0;MQSB=0.0144756;MQ0F=0 PL:DP:DV 0,21,185:7:0 0,9,92:3:0 0,18,151:6:0 17 211 . C 0 . DP=16;I16=12,4,0,0,563,20227,0,0,836,46564,0,0,265,5627,0,0;QS=3,0;MQSB=0.0144756;MQ0F=0 PL:DP:DV 0,21,172:7:0 0,9,92:3:0 0,18,153:6:0 17 212 . C 0 . DP=16;I16=12,4,0,0,589,22179,0,0,836,46564,0,0,263,5643,0,0;QS=3,0;MQSB=0.0144756;MQ0F=0 PL:DP:DV 0,21,181:7:0 0,9,92:3:0 0,18,152:6:0 17 213 . T 0 . DP=16;I16=12,4,0,0,598,22838,0,0,836,46564,0,0,262,5678,0,0;QS=3,0;MQSB=0.0144756;MQ0F=0 PL:DP:DV 0,21,181:7:0 0,9,95:3:0 0,18,165:6:0 17 214 . A 0 . DP=16;I16=11,4,0,0,529,19401,0,0,776,42964,0,0,240,5248,0,0;QS=3,0;MQSB=0.0161635;MQ0F=0 PL:DP:DV 0,21,176:7:0 0,9,92:3:0 0,15,118:5:0 17 215 . G 0 . DP=15;I16=12,3,0,0,521,19073,0,0,807,45723,0,0,262,5754,0,0;QS=3,0;MQSB=0.0342181;MQ0F=0 PL:DP:DV 0,21,185:7:0 0,9,90:3:0 0,15,105:5:0 17 216 . A 0 . DP=14;I16=10,3,0,0,464,16900,0,0,687,38523,0,0,238,5166,0,0;QS=3,0;MQSB=0.040184;MQ0F=0 PL:DP:DV 0,21,173:7:0 0,9,92:3:0 0,9,81:3:0 17 217 . A 0 . DP=14;I16=11,3,0,0,515,19433,0,0,747,42123,0,0,264,5842,0,0;QS=3,0;MQSB=0.0368832;MQ0F=0 PL:DP:DV 0,21,181:7:0 0,9,90:3:0 0,12,97:4:0 17 218 . G 0 . DP=14;I16=11,3,0,0,507,18957,0,0,747,42123,0,0,265,5907,0,0;QS=3,0;MQSB=0.0368832;MQ0F=0 PL:DP:DV 0,21,178:7:0 0,9,90:3:0 0,12,110:4:0 17 219 . T 0 . DP=14;I16=11,3,0,0,470,16286,0,0,747,42123,0,0,266,5986,0,0;QS=3,0;MQSB=0.0368832;MQ0F=0 PL:DP:DV 0,21,173:7:0 0,9,88:3:0 0,12,89:4:0 17 220 . G 0 . DP=14;I16=10,3,0,0,485,18307,0,0,687,38523,0,0,242,5454,0,0;QS=3,0;MQSB=0.040184;MQ0F=0 PL:DP:DV 0,21,188:7:0 0,9,88:3:0 0,9,80:3:0 17 221 . A 0 . DP=14;I16=11,3,0,0,487,17615,0,0,747,42123,0,0,267,6135,0,0;QS=3,0;MQSB=0.0368832;MQ0F=0 PL:DP:DV 0,21,176:7:0 0,9,88:3:0 0,12,101:4:0 17 222 . A 0 . DP=14;I16=10,3,0,0,465,17367,0,0,687,38523,0,0,242,5578,0,0;QS=3,0;MQSB=0.040184;MQ0F=0 PL:DP:DV 0,21,186:7:0 0,9,85:3:0 0,9,69:3:0 17 223 . G 0 . DP=13;I16=9,3,0,0,405,14327,0,0,627,34923,0,0,243,5657,0,0;QS=3,0;MQSB=0.0443614;MQ0F=0 PL:DP:DV 0,18,168:6:0 0,6,53:2:0 0,12,81:4:0 17 224 . G 0 . DP=12;I16=9,3,0,0,379,12759,0,0,627,34923,0,0,270,6370,0,0;QS=3,0;MQSB=0.0443614;MQ0F=0 PL:DP:DV 0,18,168:6:0 0,6,50:2:0 0,12,70:4:0 17 225 . C 0 . DP=12;I16=8,3,0,0,382,13896,0,0,567,31323,0,0,261,6345,0,0;QS=3,0;MQSB=0.0497871;MQ0F=0 PL:DP:DV 0,18,165:6:0 0,6,48:2:0 0,9,83:3:0 17 226 . A 0 . DP=13;I16=8,3,0,0,381,13669,0,0,567,31323,0,0,248,5894,0,0;QS=3,0;MQSB=0.0497871;MQ0F=0 PL:DP:DV 0,18,166:6:0 0,6,53:2:0 0,9,84:3:0 17 227 . C 0 . DP=13;I16=8,4,0,0,406,14306,0,0,596,32164,0,0,267,6253,0,0;QS=3,0;MQSB=0.0249144;MQ0F=0 PL:DP:DV 0,21,190:7:0 0,6,53:2:0 0,9,73:3:0 17 228 . C 0 . DP=13;I16=9,4,0,0,417,14381,0,0,656,35764,0,0,292,6884,0,0;QS=3,0;MQSB=0.0211283;MQ0F=0 PL:DP:DV 0,21,187:7:0 0,6,45:2:0 0,12,96:4:0 17 229 . G 0 . DP=13;I16=9,3,0,0,358,11424,0,0,627,34923,0,0,270,6414,0,0;QS=3,0;MQSB=0.0443614;MQ0F=0 PL:DP:DV 0,18,136:6:0 0,6,53:2:0 0,12,70:4:0 17 230 . C 0 . DP=13;I16=9,4,0,0,461,16861,0,0,656,35764,0,0,292,6920,0,0;QS=3,0;MQSB=0.0211283;MQ0F=0 PL:DP:DV 0,21,186:7:0 0,6,53:2:0 0,12,100:4:0 17 231 . C 0 . DP=13;I16=7,4,0,0,414,15832,0,0,536,28564,0,0,247,5925,0,0;QS=3,0;MQSB=0.0401934;MQ0F=0 PL:DP:DV 0,18,184:6:0 0,6,53:2:0 0,9,82:3:0 17 232 . C 0 . DP=14;I16=9,4,0,0,471,17371,0,0,656,35764,0,0,267,6363,0,0;QS=3,0;MQSB=0.0211283;MQ0F=0 PL:DP:DV 0,21,198:7:0 0,6,53:2:0 0,12,101:4:0 17 233 . A 0 . DP=14;I16=10,4,0,0,496,18142,0,0,716,39364,0,0,292,6984,0,0;QS=3,0;MQSB=0.0183156;MQ0F=0 PL:DP:DV 0,21,192:7:0 0,6,53:2:0 0,15,119:5:0 17 234 . A 0 . DP=14;I16=10,4,0,0,502,18390,0,0,716,39364,0,0,292,6988,0,0;QS=3,0;MQSB=0.0183156;MQ0F=0 PL:DP:DV 0,21,185:7:0 0,6,53:2:0 0,15,123:5:0 17 235 . A 0 . DP=14;I16=9,4,0,0,476,17652,0,0,656,35764,0,0,267,6375,0,0;QS=3,0;MQSB=0.0211283;MQ0F=0 PL:DP:DV 0,21,186:7:0 0,6,53:2:0 0,12,111:4:0 17 236 . G 0 . DP=15;I16=11,4,0,0,501,17481,0,0,776,42964,0,0,290,6924,0,0;QS=3,0;MQSB=0.0161635;MQ0F=0 PL:DP:DV 0,24,206:8:0 0,6,53:2:0 0,15,103:5:0 17 237 . A 0 . DP=14;I16=9,4,0,0,465,16877,0,0,656,35764,0,0,266,6282,0,0;QS=3,0;MQSB=0.0211283;MQ0F=0 PL:DP:DV 0,24,206:8:0 0,6,53:2:0 0,9,92:3:0 17 238 . C 0 . DP=14;I16=10,4,0,0,482,17238,0,0,716,39364,0,0,292,6900,0,0;QS=3,0;MQSB=0.0183156;MQ0F=0 PL:DP:DV 0,24,211:8:0 0,6,53:2:0 0,12,82:4:0 17 239 . A 0 . DP=15;I16=10,5,0,0,525,19155,0,0,776,42964,0,0,292,6852,0,0;QS=3,0;MQSB=0.0497871;MQ0F=0 PL:DP:DV 0,27,223:9:0 0,6,50:2:0 0,12,108:4:0 17 240 . C 0 . DP=15;I16=10,5,0,0,512,17930,0,0,776,42964,0,0,292,6764,0,0;QS=3,0;MQSB=0.0497871;MQ0F=0 PL:DP:DV 0,27,220:9:0 0,6,53:2:0 0,12,106:4:0 17 241 . G 0 . DP=15;I16=9,5,0,0,444,14636,0,0,716,39364,0,0,269,6159,0,0;QS=3,0;MQSB=0.0561348;MQ0F=0 PL:DP:DV 0,27,203:9:0 0,6,53:2:0 0,9,59:3:0 17 242 . C 0 . DP=15;I16=10,5,0,0,555,21177,0,0,776,42964,0,0,292,6624,0,0;QS=3,0;MQSB=0.0497871;MQ0F=0 PL:DP:DV 0,27,242:9:0 0,6,53:2:0 0,12,94:4:0 17 243 . C 0 . DP=16;I16=9,5,0,0,523,19737,0,0,716,39364,0,0,284,6508,0,0;QS=3,0;MQSB=0.0561348;MQ0F=0 PL:DP:DV 0,24,220:8:0 0,6,53:2:0 0,12,104:4:0 17 244 . C 0 . DP=16;I16=10,6,0,0,620,24272,0,0,805,43805,0,0,298,6568,0,0;QS=3,0;MQSB=0.0253122;MQ0F=0 PL:DP:DV 0,27,245:9:0 0,9,72:3:0 0,12,106:4:0 17 245 . A 0 . DP=17;I16=10,7,0,0,649,24843,0,0,865,47405,0,0,299,6553,0,0;QS=3,0;MQSB=0.0509867;MQ0F=0 PL:DP:DV 0,27,236:9:0 0,12,93:4:0 0,12,115:4:0 17 246 . T 0 . DP=18;I16=10,8,0,0,649,23833,0,0,894,48246,0,0,301,6553,0,0;QS=3,0;MQSB=0.0286491;MQ0F=0 PL:DP:DV 0,30,247:10:0 0,12,94:4:0 0,12,98:4:0 17 247 . G 0 . DP=18;I16=10,8,0,0,642,23610,0,0,894,48246,0,0,304,6570,0,0;QS=3,0;MQSB=0.0286491;MQ0F=0 PL:DP:DV 0,30,252:10:0 0,12,83:4:0 0,12,103:4:0 17 248 . T 0 . DP=18;I16=10,8,0,0,636,22944,0,0,894,48246,0,0,307,6605,0,0;QS=3,0;MQSB=0.0286491;MQ0F=0 PL:DP:DV 0,30,234:10:0 0,12,86:4:0 0,12,114:4:0 17 249 . C 0 . DP=18;I16=10,8,0,0,656,24846,0,0,894,48246,0,0,310,6658,0,0;QS=3,0;MQSB=0.0286491;MQ0F=0 PL:DP:DV 0,30,253:10:0 0,12,79:4:0 0,12,112:4:0 17 250 . C 0 . DP=19;I16=10,9,0,0,694,26160,0,0,923,49087,0,0,311,6631,0,0;QS=3,0;MQSB=0.0168512;MQ0F=0 PL:DP:DV 0,30,248:10:0 0,12,89:4:0 0,15,142:5:0 17 251 . A 0 . DP=19;I16=9,9,0,0,688,26506,0,0,863,45487,0,0,313,6627,0,0;QS=3,0;MQSB=0.0208913;MQ0F=0 PL:DP:DV 0,27,233:9:0 0,12,97:4:0 0,15,148:5:0 17 252 . G 0 . DP=18;I16=8,9,0,0,641,24631,0,0,803,41887,0,0,304,6502,0,0;QS=3,0;MQSB=0.026526;MQ0F=0 PL:DP:DV 0,27,243:9:0 0,12,91:4:0 0,12,121:4:0 17 253 . C 0 . DP=19;I16=9,10,0,0,705,26921,0,0,892,46328,0,0,319,6687,0,0;QS=3,0;MQSB=0.0132999;MQ0F=0 PL:DP:DV 0,27,247:9:0 0,12,86:4:0 0,18,155:6:0 17 254 . T 0 . DP=20;I16=10,9,0,0,719,27517,0,0,892,46328,0,0,314,6670,0,0;QS=3,0;MQSB=0.00482795;MQ0F=0 PL:DP:DV 0,30,254:10:0 0,9,72:3:0 0,18,164:6:0 17 255 . T 0 . DP=21;I16=11,10,0,0,750,27076,0,0,1012,53528,0,0,328,6840,0,0;QS=3,0;MQSB=0.00822975;MQ0F=0 PL:DP:DV 0,33,241:11:0 0,12,95:4:0 0,18,161:6:0 17 256 . A 0 . DP=22;I16=11,11,0,0,811,30063,0,0,1049,54897,0,0,334,6956,0,0;QS=3,0;MQSB=0.00507916;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,110:5:0 0,18,166:6:0 17 257 . T 0 . DP=22;I16=11,11,0,0,814,30420,0,0,1049,54897,0,0,341,7101,0,0;QS=3,0;MQSB=0.00507916;MQ0F=0 PL:DP:DV 0,33,247:11:0 0,15,113:5:0 0,18,168:6:0 17 258 . T 0 . DP=22;I16=11,11,0,0,791,28943,0,0,1049,54897,0,0,347,7225,0,0;QS=3,0;MQSB=0.00507916;MQ0F=0 PL:DP:DV 0,33,254:11:0 0,15,116:5:0 0,18,155:6:0 17 259 . C 0 . DP=22;I16=11,10,0,0,785,29809,0,0,1020,54056,0,0,332,6936,0,0;QS=3,0;MQSB=0.00822975;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,90:4:0 0,18,170:6:0 17 260 . T 0 . DP=21;I16=10,11,0,0,829,32899,0,0,989,51297,0,0,360,7556,0,0;QS=3,0;MQSB=0.00660016;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,118:5:0 0,15,156:5:0 17 261 . G 0 . DP=21;I16=10,11,0,0,735,27379,0,0,989,51297,0,0,367,7761,0,0;QS=3,0;MQSB=0.00660016;MQ0F=0 PL:DP:DV 0,33,254:11:0 0,15,111:5:0 0,15,122:5:0 17 262 . C 0 . DP=22;I16=10,12,0,0,806,30278,0,0,1049,54897,0,0,373,7941,0,0;QS=3,0;MQSB=0.0122507;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,99:5:0 0,18,164:6:0 17 263 . C 0 . DP=22;I16=10,12,0,0,799,29717,0,0,1049,54897,0,0,380,8146,0,0;QS=3,0;MQSB=0.0122507;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,98:5:0 0,18,168:6:0 17 264 . C 0 . DP=22;I16=10,12,0,0,821,31325,0,0,1049,54897,0,0,386,8326,0,0;QS=3,0;MQSB=0.0122507;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,104:5:0 0,18,172:6:0 17 265 . A 0 . DP=21;I16=9,12,0,0,800,31906,0,0,989,51297,0,0,390,8380,0,0;QS=3,0;MQSB=0.0158903;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,114:5:0 0,15,129:5:0 17 266 . G 0 . DP=21;I16=9,11,0,0,747,28155,0,0,960,50456,0,0,369,7833,0,0;QS=3,0;MQSB=0.0237479;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,97:4:0 0,15,138:5:0 17 267 . T 0 . DP=21;I16=9,11,0,0,739,27465,0,0,960,50456,0,0,373,7935,0,0;QS=3,0;MQSB=0.0237479;MQ0F=0 PL:DP:DV 0,33,254:11:0 0,12,101:4:0 0,15,149:5:0 17 268 . T 0 . DP=21;I16=9,12,0,0,748,27708,0,0,989,51297,0,0,402,8686,0,0;QS=3,0;MQSB=0.0158903;MQ0F=0 PL:DP:DV 0,33,238:11:0 0,15,110:5:0 0,15,156:5:0 17 269 . C 0 . DP=22;I16=9,12,0,0,764,28632,0,0,989,51297,0,0,381,8211,0,0;QS=3,0;MQSB=0.0158903;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,91:4:0 0,15,154:5:0 17 270 . C 0 . DP=22;I16=9,12,0,0,758,28146,0,0,989,51297,0,0,385,8337,0,0;QS=3,0;MQSB=0.0158903;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,96:4:0 0,15,143:5:0 17 271 . T 0 . DP=22;I16=9,13,0,0,847,32935,0,0,1018,52138,0,0,413,9065,0,0;QS=3,0;MQSB=0.0109431;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,113:5:0 0,15,152:5:0 17 272 . C 0 . DP=22;I16=9,12,0,0,809,31413,0,0,989,51297,0,0,390,8518,0,0;QS=3,0;MQSB=0.0158903;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,96:4:0 0,15,149:5:0 17 273 . T 0 . DP=22;I16=9,12,0,0,798,30664,0,0,989,51297,0,0,392,8620,0,0;QS=3,0;MQSB=0.0158903;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,95:4:0 0,15,161:5:0 17 274 . C 0 . DP=22;I16=9,12,0,0,763,28177,0,0,989,51297,0,0,394,8746,0,0;QS=3,0;MQSB=0.0158903;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,101:4:0 0,15,144:5:0 17 275 . C 0 . DP=20;I16=7,13,0,0,768,29994,0,0,898,44938,0,0,423,9519,0,0;QS=3,0;MQSB=0.0213617;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,114:5:0 0,12,122:4:0 17 276 . A 0 . DP=20;I16=7,13,0,0,805,32931,0,0,898,44938,0,0,424,9538,0,0;QS=3,0;MQSB=0.0213617;MQ0F=0 PL:DP:DV 0,33,253:11:0 0,15,122:5:0 0,12,124:4:0 17 277 . G 0 . DP=20;I16=7,13,0,0,764,29732,0,0,898,44938,0,0,425,9579,0,0;QS=3,0;MQSB=0.0213617;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,114:5:0 0,12,121:4:0 17 278 . A 0 . DP=21;I16=6,14,0,0,722,26452,0,0,867,42179,0,0,415,9521,0,0;QS=3,0;MQSB=0.0246228;MQ0F=0 PL:DP:DV 0,30,238:10:0 0,18,123:6:0 0,12,121:4:0 17 279 . A 0 . DP=22;I16=7,15,0,0,786,28694,0,0,956,46620,0,0,427,9677,0,0;QS=3,0;MQSB=0.0124927;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,123:6:0 0,12,122:4:0 17 280 . A 0 . DP=22;I16=7,15,0,0,815,31561,0,0,956,46620,0,0,428,9684,0,0;QS=3,0;MQSB=0.0124927;MQ0F=0 PL:DP:DV 0,36,253:12:0 0,18,130:6:0 0,12,129:4:0 17 281 . G 0 . DP=22;I16=7,15,0,0,820,31416,0,0,956,46620,0,0,428,9662,0,0;QS=3,0;MQSB=0.0124927;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,122:6:0 0,12,123:4:0 17 282 . G 0 . DP=22;I16=7,15,0,0,806,30420,0,0,956,46620,0,0,427,9609,0,0;QS=3,0;MQSB=0.0124927;MQ0F=0 PL:DP:DV 0,36,253:12:0 0,18,124:6:0 0,12,119:4:0 17 283 . C 0 . DP=23;I16=7,15,0,0,827,31785,0,0,956,46620,0,0,426,9574,0,0;QS=3,0;MQSB=0.0124927;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,125:6:0 0,12,122:4:0 17 284 . T 0 . DP=23;I16=7,16,0,0,901,35479,0,0,1016,50220,0,0,431,9593,0,0;QS=3,0;MQSB=0.0194969;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,126:6:0 0,15,144:5:0 17 285 . G 0 . DP=23;I16=7,16,0,0,860,32856,0,0,1016,50220,0,0,431,9607,0,0;QS=3,0;MQSB=0.0194969;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,119:6:0 0,15,132:5:0 17 286 . C 0 . DP=24;I16=8,16,0,0,875,32883,0,0,1076,53820,0,0,431,9641,0,0;QS=3,0;MQSB=0.0132999;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,150:7:0 0,15,134:5:0 17 287 . A 0 . DP=25;I16=9,16,0,0,895,32957,0,0,1136,57420,0,0,432,9696,0,0;QS=3,0;MQSB=0.00934348;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,24,178:8:0 0,15,133:5:0 17 288 . T 0 . DP=25;I16=9,16,0,0,931,35011,0,0,1136,57420,0,0,432,9674,0,0;QS=3,0;MQSB=0.00934348;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,24,184:8:0 0,15,146:5:0 17 289 . G 0 . DP=25;I16=9,16,0,0,939,36117,0,0,1136,57420,0,0,432,9676,0,0;QS=3,0;MQSB=0.00934348;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,24,185:8:0 0,15,136:5:0 17 290 . G 0 . DP=23;I16=8,15,0,0,805,29157,0,0,1047,52979,0,0,433,9651,0,0;QS=3,0;MQSB=0.0177152;MQ0F=0 PL:DP:DV 0,33,240:11:0 0,21,164:7:0 0,15,126:5:0 17 291 . T 0 . DP=24;I16=8,15,0,0,840,31616,0,0,1047,52979,0,0,421,9479,0,0;QS=3,0;MQSB=0.0177152;MQ0F=0 PL:DP:DV 0,33,244:11:0 0,21,168:7:0 0,15,136:5:0 17 292 . T 0 . DP=25;I16=9,16,0,0,888,32274,0,0,1167,60179,0,0,436,9668,0,0;QS=3,0;MQSB=0.0197089;MQ0F=0 PL:DP:DV 0,33,253:11:0 0,24,181:8:0 0,18,156:6:0 17 293 . G 0 . DP=26;I16=10,15,0,0,934,35232,0,0,1167,60179,0,0,424,9488,0,0;QS=3,0;MQSB=0.0095249;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,24,196:8:0 0,15,145:5:0 17 294 . A 0 . DP=26;I16=10,16,0,0,931,33937,0,0,1227,63779,0,0,443,9785,0,0;QS=3,0;MQSB=0.0149748;MQ0F=0 PL:DP:DV 0,36,252:12:0 0,24,201:8:0 0,18,161:6:0 17 295 . C 0 . DP=25;I16=10,14,0,0,897,33973,0,0,1169,62097,0,0,430,9544,0,0;QS=3,0;MQSB=0.0310726;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,21,180:7:0 0,18,159:6:0 17 296 . A 0 . DP=25;I16=10,15,0,0,874,31846,0,0,1198,62938,0,0,451,9905,0,0;QS=3,0;MQSB=0.0213617;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,169:8:0 0,18,169:6:0 17 297 . C 0 . DP=25;I16=9,15,0,0,901,34305,0,0,1138,59338,0,0,445,9901,0,0;QS=3,0;MQSB=0.0273237;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,21,174:7:0 0,18,161:6:0 17 298 . A 0 . DP=26;I16=11,15,0,0,936,34652,0,0,1258,66538,0,0,459,10121,0,0;QS=3,0;MQSB=0.017008;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,184:8:0 0,21,191:7:0 17 299 . C 0 . DP=27;I16=11,15,0,0,971,36863,0,0,1258,66538,0,0,464,10266,0,0;QS=3,0;MQSB=0.017008;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,193:8:0 0,21,189:7:0 17 300 . A 0 . DP=27;I16=11,15,0,0,1001,39455,0,0,1258,66538,0,0,469,10437,0,0;QS=3,0;MQSB=0.017008;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,204:8:0 0,21,210:7:0 17 301 . G 0 . DP=25;I16=10,14,0,0,928,36116,0,0,1169,62097,0,0,476,10632,0,0;QS=3,0;MQSB=0.0310726;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,195:7:0 0,21,196:7:0 17 302 . T 0 . DP=25;I16=10,14,0,0,879,32885,0,0,1169,62097,0,0,483,10849,0,0;QS=3,0;MQSB=0.0310726;MQ0F=0 PL:DP:DV 0,30,231:10:0 0,21,172:7:0 0,21,202:7:0 17 302 . T TA 0 . INDEL;IDV=7;IMF=1;DP=25;I16=2,4,8,11,214,7674,793,33369,236,10564,993,55133,109,2229,377,8629;QS=0.511212,2.48879;VDB=0.27613;SGB=-4.22417;MQSB=0.0443614;MQ0F=0 PL:DP:DV 167,0,96:11:6 157,0,9:7:6 201,21,0:7:7 17 303 . G 0 . DP=25;I16=10,15,0,0,976,38516,0,0,1229,65697,0,0,497,11181,0,0;QS=3,0;MQSB=0.0443614;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,21,197:7:0 0,21,195:7:0 17 304 . C 0 . DP=27;I16=11,16,0,0,991,37005,0,0,1318,70138,0,0,503,11359,0,0;QS=3,0;MQSB=0.129164;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,206:8:0 0,24,200:8:0 17 305 . C 0 . DP=27;I16=11,16,0,0,1057,41761,0,0,1318,70138,0,0,510,11508,0,0;QS=3,0;MQSB=0.129164;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,213:8:0 0,24,211:8:0 17 306 . T 0 . DP=27;I16=11,16,0,0,1033,40253,0,0,1318,70138,0,0,517,11679,0,0;QS=3,0;MQSB=0.129164;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,207:8:0 0,24,217:8:0 17 307 . G 0 . DP=27;I16=11,15,0,0,984,37886,0,0,1289,69297,0,0,498,11198,0,0;QS=3,0;MQSB=0.174566;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,21,189:7:0 0,24,203:8:0 17 308 . C 0 . DP=27;I16=11,16,0,0,892,30810,0,0,1318,70138,0,0,529,11991,0,0;QS=3,0;MQSB=0.129164;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,178:8:0 0,24,185:8:0 17 309 . G 0 . DP=27;I16=11,16,0,0,951,34599,0,0,1318,70138,0,0,535,12183,0,0;QS=3,0;MQSB=0.129164;MQ0F=0 PL:DP:DV 0,33,243:11:0 0,24,183:8:0 0,24,205:8:0 17 310 . A 0 . DP=27;I16=11,16,0,0,1001,38063,0,0,1318,70138,0,0,540,12350,0,0;QS=3,0;MQSB=0.129164;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,200:8:0 0,24,217:8:0 17 311 . C 0 . DP=27;I16=11,16,0,0,1037,40263,0,0,1318,70138,0,0,544,12492,0,0;QS=3,0;MQSB=0.129164;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,215:8:0 0,24,210:8:0 17 312 . A 0 . DP=26;I16=10,16,0,0,985,38043,0,0,1258,66538,0,0,549,12657,0,0;QS=3,0;MQSB=0.157183;MQ0F=0 PL:DP:DV 0,30,237:10:0 0,24,215:8:0 0,24,218:8:0 17 313 . A 0 . DP=26;I16=10,16,0,0,983,37969,0,0,1258,66538,0,0,551,12695,0,0;QS=3,0;MQSB=0.157183;MQ0F=0 PL:DP:DV 0,30,235:10:0 0,24,219:8:0 0,24,215:8:0 17 314 . A 0 . DP=27;I16=10,17,0,0,1050,41798,0,0,1318,70138,0,0,553,12757,0,0;QS=3,0;MQSB=0.195223;MQ0F=0 PL:DP:DV 0,33,250:11:0 0,24,217:8:0 0,24,227:8:0 17 315 . G 0 . DP=26;I16=10,16,0,0,1025,40941,0,0,1289,69297,0,0,557,12843,0,0;QS=3,0;MQSB=0.252051;MQ0F=0 PL:DP:DV 0,30,252:10:0 0,24,216:8:0 0,24,225:8:0 17 316 . C 0 . DP=27;I16=10,15,0,0,983,39393,0,0,1252,67928,0,0,535,12277,0,0;QS=3,0;MQSB=0.312403;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,183:7:0 0,24,224:8:0 17 317 . T 0 . DP=27;I16=10,16,0,0,1028,41392,0,0,1320,72056,0,0,547,12557,0,0;QS=3,0;MQSB=0.377061;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,24,230:8:0 0,24,206:8:0 17 318 . G 0 . DP=27;I16=10,17,0,0,1038,40546,0,0,1349,72897,0,0,570,13018,0,0;QS=3,0;MQSB=0.297797;MQ0F=0 PL:DP:DV 0,30,249:10:0 0,27,235:9:0 0,24,208:8:0 17 319 . A 0 . DP=27;I16=9,17,0,0,994,38654,0,0,1289,69297,0,0,560,12906,0,0;QS=3,0;MQSB=0.346864;MQ0F=0 PL:DP:DV 0,30,249:10:0 0,27,228:9:0 0,21,185:7:0 17 320 . A 0 . DP=27;I16=10,17,0,0,1022,39418,0,0,1349,72897,0,0,573,13053,0,0;QS=3,0;MQSB=0.297797;MQ0F=0 PL:DP:DV 0,30,247:10:0 0,27,230:9:0 0,24,211:8:0 17 321 . T 0 . DP=27;I16=10,17,0,0,1026,39772,0,0,1349,72897,0,0,573,13029,0,0;QS=3,0;MQSB=0.297797;MQ0F=0 PL:DP:DV 0,30,253:10:0 0,27,214:9:0 0,24,218:8:0 17 322 . G 0 . DP=28;I16=10,18,0,0,1091,43151,0,0,1409,76497,0,0,573,13029,0,0;QS=3,0;MQSB=0.343265;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,27,226:9:0 0,27,223:9:0 17 323 . C 0 . DP=28;I16=9,18,0,0,1067,42619,0,0,1349,72897,0,0,565,12939,0,0;QS=3,0;MQSB=0.394987;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,27,225:9:0 0,24,198:8:0 17 324 . T 0 . DP=30;I16=12,18,0,0,1145,44221,0,0,1529,83697,0,0,573,13001,0,0;QS=3,0;MQSB=0.264959;MQ0F=0 PL:DP:DV 0,30,253:10:0 0,27,237:9:0 0,33,255:11:0 17 325 . A 0 . DP=31;I16=13,18,0,0,1132,42058,0,0,1589,87297,0,0,573,12925,0,0;QS=3,0;MQSB=0.235201;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,208:9:0 0,33,255:11:0 17 326 . T 0 . DP=31;I16=13,18,0,0,1157,44193,0,0,1589,87297,0,0,574,12878,0,0;QS=3,0;MQSB=0.235201;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,216:9:0 0,33,255:11:0 17 327 . C 0 . DP=31;I16=13,18,0,0,1147,43895,0,0,1589,87297,0,0,575,12861,0,0;QS=3,0;MQSB=0.235201;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,198:9:0 0,33,255:11:0 17 328 . A 0 . DP=31;I16=13,18,0,0,1167,44531,0,0,1589,87297,0,0,574,12776,0,0;QS=3,0;MQSB=0.235201;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,226:9:0 0,33,255:11:0 17 329 . T 0 . DP=31;I16=13,18,0,0,1210,47742,0,0,1589,87297,0,0,572,12676,0,0;QS=3,0;MQSB=0.235201;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,237:9:0 0,33,255:11:0 17 330 . T 0 . DP=31;I16=13,18,0,0,1185,45839,0,0,1589,87297,0,0,568,12510,0,0;QS=3,0;MQSB=0.235201;MQ0F=0 PL:DP:DV 0,33,254:11:0 0,27,231:9:0 0,33,255:11:0 17 331 . T 0 . DP=32;I16=14,18,0,0,1154,42510,0,0,1649,90897,0,0,563,12327,0,0;QS=3,0;MQSB=0.210122;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,222:9:0 0,36,255:12:0 17 332 . A 0 . DP=32;I16=14,18,0,0,1156,42666,0,0,1649,90897,0,0,560,12178,0,0;QS=3,0;MQSB=0.210122;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,214:9:0 0,33,255:11:0 17 333 . A 0 . DP=32;I16=14,18,0,0,1141,41987,0,0,1649,90897,0,0,558,12064,0,0;QS=3,0;MQSB=0.210122;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,223:9:0 0,33,255:11:0 17 334 . A 0 . DP=32;I16=14,18,0,0,1162,43328,0,0,1649,90897,0,0,556,11986,0,0;QS=3,0;MQSB=0.210122;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,221:9:0 0,33,255:11:0 17 335 . A 0 . DP=32;I16=12,18,0,0,1077,40287,0,0,1529,83697,0,0,552,11934,0,0;QS=3,0;MQSB=0.264959;MQ0F=0 PL:DP:DV 0,30,250:10:0 0,27,219:9:0 0,33,251:11:0 17 336 . A 0 . DP=32;I16=14,17,0,0,1088,39758,0,0,1612,89528,0,0,536,11574,0,0;QS=3,0;MQSB=0.274662;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,211:8:0 0,36,255:12:0 17 337 . C 0 . DP=32;I16=13,17,0,0,1115,42381,0,0,1552,85928,0,0,531,11565,0,0;QS=3,0;MQSB=0.301511;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,24,202:8:0 0,36,255:12:0 17 338 . T 0 . DP=30;I16=14,16,0,0,1191,47979,0,0,1560,86456,0,0,554,11878,0,0;QS=3,0;MQSB=0.242376;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,24,226:8:0 0,36,255:12:0 17 339 . C 0 . DP=31;I16=14,17,0,0,1130,43210,0,0,1589,87297,0,0,554,11874,0,0;QS=3,0;MQSB=0.175471;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,185:8:0 0,36,255:12:0 17 340 . C 0 . DP=31;I16=14,17,0,0,1196,47044,0,0,1589,87297,0,0,554,11852,0,0;QS=3,0;MQSB=0.175471;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,221:8:0 0,36,255:12:0 17 341 . T 0 . DP=31;I16=14,17,0,0,1227,48995,0,0,1589,87297,0,0,554,11862,0,0;QS=3,0;MQSB=0.175471;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,216:8:0 0,36,255:12:0 17 342 . T 0 . DP=31;I16=14,17,0,0,1162,43942,0,0,1589,87297,0,0,554,11904,0,0;QS=3,0;MQSB=0.175471;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,210:8:0 0,36,255:12:0 17 343 . G 0 . DP=32;I16=14,17,0,0,1150,43702,0,0,1620,90056,0,0,550,11962,0,0;QS=3,0;MQSB=0.283511;MQ0F=0 PL:DP:DV 0,30,249:10:0 0,27,218:9:0 0,36,255:12:0 17 344 . C 0 . DP=32;I16=14,18,0,0,1181,45169,0,0,1649,90897,0,0,554,12036,0,0;QS=3,0;MQSB=0.210122;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,217:9:0 0,36,255:12:0 17 345 . T 0 . DP=31;I16=14,17,0,0,1205,47259,0,0,1589,87297,0,0,555,12129,0,0;QS=3,0;MQSB=0.175471;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,221:8:0 0,36,255:12:0 17 346 . G 0 . DP=31;I16=15,16,0,0,1147,43597,0,0,1620,90056,0,0,557,12255,0,0;QS=3,0;MQSB=0.220358;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,212:8:0 0,36,255:12:0 17 347 . G 0 . DP=31;I16=14,16,0,0,1119,42227,0,0,1560,86456,0,0,545,12189,0,0;QS=3,0;MQSB=0.242376;MQ0F=0 PL:DP:DV 0,30,251:10:0 0,24,207:8:0 0,36,255:12:0 17 348 . T 0 . DP=32;I16=15,16,0,0,1145,43007,0,0,1620,90056,0,0,546,12300,0,0;QS=3,0;MQSB=0.220358;MQ0F=0 PL:DP:DV 0,30,243:10:0 0,27,228:9:0 0,36,255:12:0 17 349 . T 0 . DP=32;I16=16,16,0,0,1194,45350,0,0,1680,93656,0,0,565,12731,0,0;QS=3,0;MQSB=0.201402;MQ0F=0 PL:DP:DV 0,33,246:11:0 0,27,230:9:0 0,36,255:12:0 17 350 . T 0 . DP=31;I16=16,15,0,0,1142,43072,0,0,1651,92815,0,0,567,12837,0,0;QS=3,0;MQSB=0.286505;MQ0F=0 PL:DP:DV 0,33,249:11:0 0,27,230:9:0 0,33,255:11:0 17 351 . G 0 . DP=31;I16=16,15,0,0,1146,43750,0,0,1651,92815,0,0,568,12920,0,0;QS=3,0;MQSB=0.286505;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,212:9:0 0,33,255:11:0 17 352 . A 0 . DP=31;I16=16,14,0,0,1150,45520,0,0,1591,89215,0,0,544,12404,0,0;QS=3,0;MQSB=0.242376;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,224:8:0 0,33,255:11:0 17 353 . G 0 . DP=29;I16=15,14,0,0,1109,43095,0,0,1562,88374,0,0,570,13064,0,0;QS=3,0;MQSB=0.424373;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,27,231:9:0 0,30,255:10:0 17 354 . A 0 . DP=28;I16=14,14,0,0,1078,42938,0,0,1502,84774,0,0,571,13075,0,0;QS=3,0;MQSB=0.450096;MQ0F=0 PL:DP:DV 0,27,225:9:0 0,27,244:9:0 0,30,255:10:0 17 355 . G T, 0 . DP=28;I16=14,13,0,1,1001,37907,41,1681,1442,81174,60,3600,547,12487,25,625;QS=2.875,0.125,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.450096;BQB=1;MQ0F=0 PL:DP:DV 14,0,200,38,203,231:9:1 0,27,222,27,222,222:9:0 0,30,255,30,255,255:10:0 17 356 . G 0 . DP=27;I16=14,13,0,0,993,37481,0,0,1465,83405,0,0,574,13174,0,0;QS=3,0;MQSB=0.580277;MQ0F=0 PL:DP:DV 0,27,228:9:0 0,24,201:8:0 0,30,255:10:0 17 357 . C 0 . DP=28;I16=14,13,0,0,1021,39471,0,0,1465,83405,0,0,550,12584,0,0;QS=3,0;MQSB=0.580277;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,24,205:8:0 0,27,251:9:0 17 358 . A 0 . DP=28;I16=15,13,0,0,1050,40518,0,0,1525,87005,0,0,576,13216,0,0;QS=3,0;MQSB=0.556581;MQ0F=0 PL:DP:DV 0,30,254:10:0 0,24,197:8:0 0,30,255:10:0 17 359 . G 0 . DP=29;I16=15,13,0,0,1085,42761,0,0,1525,87005,0,0,552,12620,0,0;QS=3,0;MQSB=0.556581;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,187:7:0 0,33,255:11:0 17 360 . A 0 . DP=29;I16=15,14,0,0,1111,43259,0,0,1585,90605,0,0,579,13297,0,0;QS=3,0;MQSB=0.604224;MQ0F=0 PL:DP:DV 0,30,252:10:0 0,24,220:8:0 0,33,255:11:0 17 361 . A 0 . DP=29;I16=15,14,0,0,1116,43442,0,0,1585,90605,0,0,579,13273,0,0;QS=3,0;MQSB=0.604224;MQ0F=0 PL:DP:DV 0,30,252:10:0 0,24,219:8:0 0,33,255:11:0 17 362 . A 0 . DP=29;I16=16,13,0,0,1104,42700,0,0,1585,90605,0,0,580,13272,0,0;QS=3,0;MQSB=0.535133;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,218:8:0 0,30,255:10:0 17 363 . A 0 . DP=29;I16=16,13,0,0,1087,41437,0,0,1585,90605,0,0,581,13245,0,0;QS=3,0;MQSB=0.535133;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,213:8:0 0,30,255:10:0 17 364 . T 0 . DP=29;I16=16,13,0,0,1032,37960,0,0,1585,90605,0,0,582,13244,0,0;QS=3,0;MQSB=0.535133;MQ0F=0 PL:DP:DV 0,33,250:11:0 0,24,205:8:0 0,30,255:10:0 17 365 . G 0 . DP=29;I16=16,13,0,0,1105,43079,0,0,1585,90605,0,0,582,13218,0,0;QS=3,0;MQSB=0.535133;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,211:8:0 0,30,255:10:0 17 366 . A 0 . DP=29;I16=16,13,0,0,1090,41562,0,0,1585,90605,0,0,581,13167,0,0;QS=3,0;MQSB=0.535133;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,211:8:0 0,30,255:10:0 17 367 . T 0 . DP=29;I16=16,13,0,0,1055,39149,0,0,1585,90605,0,0,579,13093,0,0;QS=3,0;MQSB=0.535133;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,204:8:0 0,30,255:10:0 17 368 . A 0 . DP=29;I16=16,13,0,0,1054,39632,0,0,1585,90605,0,0,576,12998,0,0;QS=3,0;MQSB=0.535133;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,208:8:0 0,30,255:10:0 17 369 . T 0 . DP=28;I16=16,11,0,0,1037,40275,0,0,1496,86164,0,0,548,12256,0,0;QS=3,0;MQSB=0.659218;MQ0F=0 PL:DP:DV 0,30,250:10:0 0,21,196:7:0 0,30,255:10:0 17 370 . C 0 . DP=28;I16=16,12,0,0,1045,40219,0,0,1556,89764,0,0,570,12790,0,0;QS=3,0;MQSB=0.705296;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,24,201:8:0 0,30,255:10:0 17 371 . T 0 . DP=29;I16=16,13,0,0,1155,46591,0,0,1616,93364,0,0,567,12725,0,0;QS=3,0;MQSB=0.744925;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,24,227:8:0 0,33,255:11:0 17 372 . C 0 . DP=30;I16=16,14,0,0,1139,44019,0,0,1676,96964,0,0,564,12636,0,0;QS=3,0;MQSB=0.779025;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,215:8:0 0,33,255:11:0 17 373 . A 0 . DP=30;I16=16,14,0,0,1142,44118,0,0,1676,96964,0,0,561,12525,0,0;QS=3,0;MQSB=0.779025;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,220:8:0 0,33,255:11:0 17 374 . T 0 . DP=30;I16=16,14,0,0,1098,41180,0,0,1676,96964,0,0,556,12344,0,0;QS=3,0;MQSB=0.779025;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,221:8:0 0,33,255:11:0 17 375 . A T, 0 . DP=31;I16=17,13,0,1,1138,43798,14,196,1676,96964,60,3600,547,12177,4,16;QS=2.9661,0.0338983,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.763662;BQB=1;MQ0F=0 PL:DP:DV 0,36,255,36,255,255:12:0 0,24,218,24,218,218:8:0 0,18,255,30,255,255:11:1 17 376 . G 0 . DP=31;I16=17,14,0,0,1131,42581,0,0,1736,100564,0,0,547,12073,0,0;QS=3,0;MQSB=0.763662;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,24,220:8:0 0,33,255:11:0 17 377 . T 0 . DP=31;I16=16,14,0,0,1105,41629,0,0,1676,96964,0,0,518,11360,0,0;QS=3,0;MQSB=0.779025;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,211:8:0 0,33,255:11:0 17 378 . T 0 . DP=30;I16=18,12,0,0,1098,41066,0,0,1707,99723,0,0,541,11927,0,0;QS=3,0;MQSB=0.878946;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,186:7:0 0,30,255:10:0 17 379 . G 0 . DP=29;I16=18,10,0,0,1053,40181,0,0,1618,95282,0,0,534,11848,0,0;QS=3,0;MQSB=0.981777;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,21,187:7:0 0,30,255:10:0 17 380 . C 0 . DP=29;I16=18,10,0,0,1087,42743,0,0,1618,95282,0,0,514,11172,0,0;QS=3,0;MQSB=0.981777;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,177:6:0 0,30,255:10:0 17 381 . T 0 . DP=29;I16=18,11,0,0,1168,47412,0,0,1678,98882,0,0,537,11729,0,0;QS=3,0;MQSB=0.987702;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,212:7:0 0,30,255:10:0 17 382 . T 0 . DP=29;I16=17,11,0,0,1054,40450,0,0,1618,95282,0,0,510,11068,0,0;QS=3,0;MQSB=0.990092;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,21,182:7:0 0,30,255:10:0 17 383 . T 0 . DP=29;I16=18,10,0,0,1052,39798,0,0,1618,95282,0,0,507,11013,0,0;QS=3,0;MQSB=0.981777;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,165:6:0 0,30,255:10:0 17 384 . A 0 . DP=31;I16=19,11,0,0,1077,39885,0,0,1738,102482,0,0,504,10988,0,0;QS=3,0;MQSB=0.985292;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,176:7:0 0,30,255:10:0 17 385 . C 0 . DP=31;I16=19,12,0,0,1118,41180,0,0,1798,106082,0,0,527,11569,0,0;QS=3,0;MQSB=0.989977;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,24,186:8:0 0,30,255:10:0 17 386 . T 0 . DP=30;I16=18,12,0,0,1158,45592,0,0,1738,102482,0,0,526,11556,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,191:7:0 0,30,255:10:0 17 387 . T 0 . DP=30;I16=18,12,0,0,1105,41821,0,0,1738,102482,0,0,525,11573,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,187:7:0 0,30,255:10:0 17 388 . T 0 . DP=29;I16=17,12,0,0,1089,41577,0,0,1678,98882,0,0,523,11519,0,0;QS=3,0;MQSB=0.993891;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,180:6:0 0,30,255:10:0 17 389 . G 0 . DP=29;I16=17,12,0,0,1067,40095,0,0,1678,98882,0,0,520,11444,0,0;QS=3,0;MQSB=0.993891;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,171:6:0 0,30,255:10:0 17 390 . C 0 . DP=29;I16=17,12,0,0,1071,40423,0,0,1678,98882,0,0,517,11399,0,0;QS=3,0;MQSB=0.993891;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,162:6:0 0,30,255:10:0 17 391 . A 0 . DP=29;I16=18,11,0,0,1091,41603,0,0,1647,96123,0,0,515,11383,0,0;QS=3,0;MQSB=0.995968;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,163:6:0 0,30,255:10:0 17 392 . T 0 . DP=29;I16=18,11,0,0,1046,38838,0,0,1647,96123,0,0,515,11395,0,0;QS=3,0;MQSB=0.995968;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,153:5:0 0,33,255:11:0 17 393 . A 0 . DP=28;I16=17,11,0,0,1014,37582,0,0,1587,92523,0,0,517,11435,0,0;QS=3,0;MQSB=0.993109;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,133:5:0 0,33,255:11:0 17 394 . T 0 . DP=28;I16=17,11,0,0,1022,38342,0,0,1587,92523,0,0,519,11503,0,0;QS=3,0;MQSB=0.993109;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,141:5:0 0,33,255:11:0 17 395 . T 0 . DP=28;I16=17,11,0,0,1060,40596,0,0,1587,92523,0,0,521,11599,0,0;QS=3,0;MQSB=0.993109;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,164:5:0 0,33,255:11:0 17 396 . T 0 . DP=28;I16=17,11,0,0,1032,39228,0,0,1587,92523,0,0,523,11723,0,0;QS=3,0;MQSB=0.993109;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,158:5:0 0,33,255:11:0 17 397 . T 0 . DP=28;I16=17,11,0,0,1046,39510,0,0,1587,92523,0,0,524,11824,0,0;QS=3,0;MQSB=0.993109;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,149:5:0 0,33,255:11:0 17 398 . A 0 . DP=28;I16=17,11,0,0,1021,38105,0,0,1587,92523,0,0,524,11850,0,0;QS=3,0;MQSB=0.993109;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,144:5:0 0,30,255:10:0 17 399 . A 0 . DP=28;I16=17,11,0,0,1015,38469,0,0,1587,92523,0,0,526,11900,0,0;QS=3,0;MQSB=0.993109;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,140:5:0 0,30,255:10:0 17 400 . A 0 . DP=29;I16=17,12,0,0,1056,39702,0,0,1647,96123,0,0,526,11828,0,0;QS=3,0;MQSB=0.988062;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,159:5:0 0,33,255:11:0 17 401 . A 0 . DP=29;I16=17,11,0,0,1052,40302,0,0,1587,92523,0,0,501,11113,0,0;QS=3,0;MQSB=0.993109;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,160:5:0 0,30,255:10:0 17 402 . T 0 . DP=29;I16=17,12,0,0,1082,41232,0,0,1647,96123,0,0,526,11680,0,0;QS=3,0;MQSB=0.988062;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,149:5:0 0,33,255:11:0 17 403 . T 0 . DP=29;I16=17,12,0,0,1085,40985,0,0,1647,96123,0,0,526,11654,0,0;QS=3,0;MQSB=0.988062;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,148:5:0 0,33,255:11:0 17 404 . G 0 . DP=29;I16=17,12,0,0,1074,40524,0,0,1647,96123,0,0,525,11609,0,0;QS=3,0;MQSB=0.988062;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,131:5:0 0,33,255:11:0 17 405 . T 0 . DP=27;I16=16,10,0,0,988,37870,0,0,1498,88082,0,0,519,11543,0,0;QS=3,0;MQSB=0.987578;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,103:3:0 0,30,255:10:0 17 406 . G 0 . DP=27;I16=16,11,0,0,976,36752,0,0,1558,91682,0,0,527,11601,0,0;QS=3,0;MQSB=0.992419;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,125:4:0 0,30,247:10:0 17 407 . A 0 . DP=27;I16=16,11,0,0,1007,38355,0,0,1558,91682,0,0,526,11538,0,0;QS=3,0;MQSB=0.992419;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,125:4:0 0,30,255:10:0 17 408 . C 0 . DP=28;I16=16,11,0,0,1006,38136,0,0,1558,91682,0,0,521,11489,0,0;QS=3,0;MQSB=0.992419;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,110:3:0 0,30,244:10:0 17 409 . T 0 . DP=29;I16=17,12,0,0,1100,42734,0,0,1678,98882,0,0,525,11503,0,0;QS=3,0;MQSB=0.993891;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,131:4:0 0,33,255:11:0 17 410 . T 0 . DP=29;I16=17,12,0,0,1035,38325,0,0,1678,98882,0,0,524,11432,0,0;QS=3,0;MQSB=0.993891;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,125:4:0 0,33,255:11:0 17 411 . T 0 . DP=29;I16=17,10,0,0,1003,37747,0,0,1558,91682,0,0,496,10716,0,0;QS=3,0;MQSB=0.984677;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,104:3:0 0,30,255:10:0 17 412 . C T, 0 . DP=30;I16=17,12,1,0,1094,42458,14,196,1678,98882,60,3600,495,10659,25,625;QS=2.97455,0.0254545,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.991968;BQB=1;MQ0F=0 PL:DP:DV 0,30,255,42,255,255:15:1 0,12,124,12,124,124:4:0 0,33,255,33,255,255:11:0 17 413 . A 0 . DP=31;I16=18,13,0,0,1189,45985,0,0,1798,106082,0,0,520,11258,0,0;QS=3,0;MQSB=0.995005;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,9,102:3:0 0,33,255:11:0 17 414 . T 0 . DP=30;I16=18,12,0,0,1151,44355,0,0,1738,102482,0,0,523,11265,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,109:3:0 0,33,255:11:0 17 415 . G 0 . DP=30;I16=18,12,0,0,1131,43175,0,0,1738,102482,0,0,526,11306,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,110:3:0 0,33,255:11:0 17 416 . G 0 . DP=30;I16=17,12,0,0,1083,41273,0,0,1678,98882,0,0,514,11156,0,0;QS=3,0;MQSB=0.993891;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,114:3:0 0,30,253:10:0 17 417 . C 0 . DP=30;I16=18,12,0,0,1114,42244,0,0,1738,102482,0,0,531,11439,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,108:3:0 0,33,255:11:0 17 418 . A 0 . DP=30;I16=18,12,0,0,1146,44248,0,0,1738,102482,0,0,532,11478,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,111:3:0 0,33,255:11:0 17 419 . T 0 . DP=30;I16=18,12,0,0,1117,42327,0,0,1738,102482,0,0,532,11498,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,112:3:0 0,33,255:11:0 17 420 . A 0 . DP=31;I16=18,13,0,0,1117,41011,0,0,1798,106082,0,0,532,11550,0,0;QS=3,0;MQSB=0.995005;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,108:3:0 0,36,255:12:0 17 421 . A 0 . DP=33;I16=19,14,0,0,1208,45398,0,0,1887,110523,0,0,533,11635,0,0;QS=3,0;MQSB=0.986656;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,113:3:0 0,42,255:14:0 17 422 . A 0 . DP=33;I16=19,13,0,0,1205,46441,0,0,1827,106923,0,0,510,11082,0,0;QS=3,0;MQSB=0.991023;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,116:3:0 0,39,255:13:0 17 423 . T 0 . DP=32;I16=19,13,0,0,1202,45416,0,0,1827,106923,0,0,538,11818,0,0;QS=3,0;MQSB=0.991023;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,110:3:0 0,39,255:13:0 17 424 . A 0 . DP=32;I16=19,13,0,0,1147,41685,0,0,1827,106923,0,0,539,11867,0,0;QS=3,0;MQSB=0.991023;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,106:3:0 0,39,255:13:0 17 425 . A 0 . DP=29;I16=16,13,0,0,1070,40616,0,0,1647,96123,0,0,542,11900,0,0;QS=3,0;MQSB=0.976248;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,111:3:0 0,33,249:11:0 17 426 . T 0 . DP=29;I16=16,12,0,0,997,36561,0,0,1587,92523,0,0,519,11287,0,0;QS=3,0;MQSB=0.982906;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,105:3:0 0,30,225:10:0 17 427 . A 0 . DP=29;I16=16,13,0,0,1024,37266,0,0,1647,96123,0,0,546,11952,0,0;QS=3,0;MQSB=0.976248;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,111:3:0 0,33,242:11:0 17 428 . C 0 . DP=29;I16=16,13,0,0,1064,39706,0,0,1647,96123,0,0,548,12020,0,0;QS=3,0;MQSB=0.976248;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,111:3:0 0,33,254:11:0 17 429 . T 0 . DP=30;I16=16,14,0,0,1150,44918,0,0,1707,99723,0,0,549,12067,0,0;QS=3,0;MQSB=0.969373;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,115:3:0 0,33,255:11:0 17 430 . G 0 . DP=30;I16=16,14,0,0,1113,42443,0,0,1707,99723,0,0,551,12145,0,0;QS=3,0;MQSB=0.969373;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,112:3:0 0,33,246:11:0 17 431 . G 0 . DP=30;I16=14,14,0,0,1003,36953,0,0,1587,92523,0,0,553,12255,0,0;QS=3,0;MQSB=0.949591;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,101:3:0 0,30,225:10:0 17 432 . T 0 . DP=28;I16=14,14,0,0,1049,39621,0,0,1587,92523,0,0,556,12346,0,0;QS=3,0;MQSB=0.949591;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,114:3:0 0,30,255:10:0 17 433 . T 0 . DP=28;I16=14,12,0,0,949,35443,0,0,1467,85323,0,0,509,11217,0,0;QS=3,0;MQSB=0.967472;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,112:3:0 0,27,227:9:0 17 434 . T 0 . DP=29;I16=15,14,0,0,1036,37654,0,0,1647,96123,0,0,561,12567,0,0;QS=3,0;MQSB=0.960561;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,98:3:0 0,30,243:10:0 17 435 . A 0 . DP=29;I16=15,13,0,0,1024,37970,0,0,1587,92523,0,0,556,12560,0,0;QS=3,0;MQSB=0.968414;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,103:3:0 0,30,237:10:0 17 436 . T 0 . DP=28;I16=14,14,0,0,998,36220,0,0,1587,92523,0,0,564,12654,0,0;QS=3,0;MQSB=0.949591;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,110:3:0 0,27,223:9:0 17 437 . T 0 . DP=28;I16=13,14,0,0,990,36832,0,0,1558,91682,0,0,549,12435,0,0;QS=3,0;MQSB=0.999706;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,109:3:0 0,24,207:8:0 17 438 . A 0 . DP=28;I16=14,13,0,0,972,35640,0,0,1527,88923,0,0,540,12082,0,0;QS=3,0;MQSB=0.9585;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,107:3:0 0,24,216:8:0 17 439 . C 0 . DP=28;I16=14,14,0,0,1055,40273,0,0,1587,92523,0,0,563,12649,0,0;QS=3,0;MQSB=0.949591;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,107:3:0 0,27,224:9:0 17 440 . A 0 . DP=28;I16=14,14,0,0,1095,43251,0,0,1587,92523,0,0,561,12615,0,0;QS=3,0;MQSB=0.949591;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,115:3:0 0,27,247:9:0 17 441 . G 0 . DP=29;I16=15,14,0,0,1068,40344,0,0,1647,96123,0,0,559,12605,0,0;QS=3,0;MQSB=0.960561;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,9,104:3:0 0,27,198:9:0 17 442 . A 0 . DP=29;I16=15,14,0,0,1091,41507,0,0,1647,96123,0,0,558,12620,0,0;QS=3,0;MQSB=0.960561;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,9,112:3:0 0,27,233:9:0 17 443 . A 0 . DP=30;I16=15,14,0,0,1173,49439,0,0,1647,96123,0,0,557,12661,0,0;QS=3,0;MQSB=0.960561;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,9,129:3:0 0,27,246:9:0 17 444 . G 0 . DP=29;I16=15,13,0,0,1095,44661,0,0,1587,92523,0,0,557,12727,0,0;QS=3,0;MQSB=0.968414;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,91:2:0 0,27,227:9:0 17 445 . C 0 . DP=30;I16=16,13,0,0,1100,43706,0,0,1647,96123,0,0,557,12817,0,0;QS=3,0;MQSB=0.976248;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,9,111:3:0 0,27,219:9:0 17 446 . A 0 . DP=30;I16=16,13,0,0,1107,44265,0,0,1647,96123,0,0,557,12881,0,0;QS=3,0;MQSB=0.976248;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,9,115:3:0 0,27,232:9:0 17 447 . C 0 . DP=29;I16=16,12,0,0,1108,45364,0,0,1618,95282,0,0,555,12817,0,0;QS=3,0;MQSB=0.856268;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,114:3:0 0,27,235:9:0 17 448 . T 0 . DP=29;I16=16,12,0,0,1125,47237,0,0,1618,95282,0,0,553,12773,0,0;QS=3,0;MQSB=0.856268;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,118:3:0 0,27,240:9:0 17 449 . A 0 . DP=28;I16=15,12,0,0,1091,45981,0,0,1558,91682,0,0,552,12748,0,0;QS=3,0;MQSB=0.84246;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,90:2:0 0,27,245:9:0 17 450 . G 0 . DP=28;I16=15,12,0,0,1069,44603,0,0,1558,91682,0,0,551,12741,0,0;QS=3,0;MQSB=0.84246;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,91:2:0 0,27,233:9:0 17 451 . A 0 . DP=28;I16=15,12,0,0,1021,41371,0,0,1558,91682,0,0,550,12752,0,0;QS=3,0;MQSB=0.84246;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,93:2:0 0,27,244:9:0 17 452 . A 0 . DP=31;I16=18,11,0,0,1079,43353,0,0,1678,98882,0,0,530,12420,0,0;QS=3,0;MQSB=0.884952;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,9,110:3:0 0,24,225:8:0 17 453 . A 0 . DP=31;I16=17,11,0,0,1037,41069,0,0,1649,98041,0,0,508,11882,0,0;QS=3,0;MQSB=0.967085;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,9,111:3:0 0,21,221:7:0 17 454 . A 0 . DP=31;I16=18,12,0,0,1158,47028,0,0,1738,102482,0,0,554,12904,0,0;QS=3,0;MQSB=0.878946;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,9,113:3:0 0,30,255:10:0 17 455 . T 0 . DP=32;I16=17,13,0,0,1148,46574,0,0,1715,100251,0,0,550,12864,0,0;QS=3,0;MQSB=0.973855;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,113:3:0 0,33,255:11:0 17 456 . G 0 . DP=32;I16=17,13,0,0,1161,47287,0,0,1746,103010,0,0,534,12296,0,0;QS=3,0;MQSB=0.998031;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,9,116:3:0 0,30,245:10:0 17 457 . C 0 . DP=33;I16=19,13,0,0,1218,48642,0,0,1835,107451,0,0,563,12967,0,0;QS=3,0;MQSB=0.985204;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,9,118:3:0 0,33,255:11:0 17 458 . A 0 . DP=33;I16=19,13,0,0,1226,49034,0,0,1835,107451,0,0,568,12990,0,0;QS=3,0;MQSB=0.985204;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,9,111:3:0 0,33,255:11:0 17 459 . T 0 . DP=33;I16=18,13,0,0,1167,46981,0,0,1775,103851,0,0,565,12945,0,0;QS=3,0;MQSB=0.980167;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,92:2:0 0,33,255:11:0 17 460 . G 0 . DP=32;I16=19,12,0,0,1219,50105,0,0,1775,103851,0,0,575,12929,0,0;QS=3,0;MQSB=0.989977;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,9,116:3:0 0,30,255:10:0 17 461 . T 0 . DP=32;I16=19,12,0,0,1213,49819,0,0,1775,103851,0,0,577,12845,0,0;QS=3,0;MQSB=0.989977;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,9,115:3:0 0,30,255:10:0 17 462 . G 0 . DP=32;I16=19,12,0,0,1190,48962,0,0,1775,103851,0,0,580,12792,0,0;QS=3,0;MQSB=0.989977;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,119:4:0 0,30,241:10:0 17 463 . G 0 . DP=32;I16=19,12,0,0,1114,44214,0,0,1775,103851,0,0,584,12770,0,0;QS=3,0;MQSB=0.989977;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,114:4:0 0,30,221:10:0 17 464 . A 0 . DP=32;I16=18,11,0,0,1100,43908,0,0,1686,99410,0,0,556,12106,0,0;QS=3,0;MQSB=0.99095;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,133:4:0 0,24,213:8:0 17 465 . C 0 . DP=33;I16=20,11,0,0,1191,48085,0,0,1775,103851,0,0,586,12786,0,0;QS=3,0;MQSB=0.996597;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,15,140:5:0 0,27,231:9:0 17 466 . A 0 . DP=34;I16=21,12,0,0,1293,53311,0,0,1895,111051,0,0,597,12897,0,0;QS=3,0;MQSB=0.995633;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,15,154:5:0 0,30,255:10:0 17 467 . A 0 . DP=34;I16=21,11,0,0,1256,51450,0,0,1835,107451,0,0,597,12891,0,0;QS=3,0;MQSB=0.998231;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,15,157:5:0 0,27,248:9:0 17 468 . A 0 . DP=35;I16=22,12,0,0,1274,51268,0,0,1955,114651,0,0,604,12904,0,0;QS=3,0;MQSB=0.997406;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,154:5:0 0,30,251:10:0 17 469 . A 0 . DP=35;I16=22,12,0,0,1285,52989,0,0,1955,114651,0,0,608,12940,0,0;QS=3,0;MQSB=0.997406;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,146:5:0 0,30,255:10:0 17 470 . G 0 . DP=35;I16=22,12,0,0,1281,51055,0,0,1955,114651,0,0,612,13016,0,0;QS=3,0;MQSB=0.997406;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,148:5:0 0,30,238:10:0 17 471 . T 0 . DP=36;I16=22,11,0,0,1239,49021,0,0,1918,113282,0,0,599,12825,0,0;QS=3,0;MQSB=0.915545;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,150:5:0 0,27,232:9:0 17 472 . T 0 . DP=35;I16=21,12,0,0,1245,48915,0,0,1926,113810,0,0,595,12559,0,0;QS=3,0;MQSB=0.988858;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,153:5:0 0,27,237:9:0 17 473 . G 0 . DP=35;I16=21,12,0,0,1307,53473,0,0,1926,113810,0,0,599,12651,0,0;QS=3,0;MQSB=0.988858;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,141:5:0 0,27,249:9:0 17 474 . G 0 . DP=36;I16=22,12,0,0,1284,51708,0,0,1986,117410,0,0,602,12734,0,0;QS=3,0;MQSB=0.986937;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,131:5:0 0,30,255:10:0 17 475 . G 0 . DP=36;I16=23,12,0,0,1311,51609,0,0,2015,118251,0,0,631,13485,0,0;QS=3,0;MQSB=0.998642;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,141:5:0 0,33,252:11:0 17 476 . A 0 . DP=36;I16=23,12,0,0,1312,52078,0,0,2015,118251,0,0,634,13606,0,0;QS=3,0;MQSB=0.998642;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,157:5:0 0,33,255:11:0 17 477 . T 0 . DP=36;I16=23,12,0,0,1318,52668,0,0,2015,118251,0,0,637,13773,0,0;QS=3,0;MQSB=0.998642;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,148:5:0 0,33,255:11:0 17 478 . T 0 . DP=38;I16=25,12,0,0,1338,51774,0,0,2135,125451,0,0,637,13833,0,0;QS=3,0;MQSB=0.999868;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,18,154:6:0 0,33,255:11:0 17 479 . A 0 . DP=38;I16=25,12,0,0,1420,57788,0,0,2135,125451,0,0,639,13935,0,0;QS=3,0;MQSB=0.999868;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,18,163:6:0 0,33,255:11:0 17 480 . G 0 . DP=37;I16=25,11,0,0,1438,60172,0,0,2075,121851,0,0,641,14029,0,0;QS=3,0;MQSB=0.999853;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,18,165:6:0 0,33,255:11:0 17 481 . G 0 . DP=37;I16=25,11,0,0,1392,55824,0,0,2075,121851,0,0,642,14112,0,0;QS=3,0;MQSB=0.999853;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,18,165:6:0 0,33,255:11:0 17 482 . A 0 . DP=37;I16=24,11,0,0,1352,55134,0,0,2015,118251,0,0,618,13608,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,143:5:0 0,33,255:11:0 17 483 . G 0 . DP=37;I16=24,12,0,0,1417,57747,0,0,2075,121851,0,0,642,14240,0,0;QS=3,0;MQSB=0.999437;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,18,165:6:0 0,33,255:11:0 17 484 . A 0 . DP=36;I16=24,11,0,0,1340,53992,0,0,2015,118251,0,0,643,14281,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,168:6:0 0,33,255:11:0 17 485 . G 0 . DP=35;I16=23,12,0,0,1329,51411,0,0,2015,118251,0,0,669,14931,0,0;QS=3,0;MQSB=0.998642;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,160:6:0 0,33,255:11:0 17 486 . A 0 . DP=34;I16=22,12,0,0,1311,51523,0,0,1955,114651,0,0,671,14989,0,0;QS=3,0;MQSB=0.997406;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,173:6:0 0,33,255:11:0 17 487 . G 0 . DP=34;I16=22,12,0,0,1306,50760,0,0,1955,114651,0,0,672,15030,0,0;QS=3,0;MQSB=0.997406;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,169:6:0 0,33,255:11:0 17 488 . A 0 . DP=35;I16=22,12,0,0,1274,48140,0,0,1986,117410,0,0,646,14380,0,0;QS=3,0;MQSB=0.986937;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,177:6:0 0,30,255:10:0 17 489 . A 0 . DP=35;I16=23,12,0,0,1264,46916,0,0,2015,118251,0,0,671,15015,0,0;QS=3,0;MQSB=0.998642;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,175:6:0 0,33,255:11:0 17 490 . A 0 . DP=36;I16=24,12,0,0,1332,50280,0,0,2075,121851,0,0,671,15061,0,0;QS=3,0;MQSB=0.999437;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,188:7:0 0,33,255:11:0 17 491 . T 0 . DP=36;I16=24,12,0,0,1284,46802,0,0,2075,121851,0,0,671,15093,0,0;QS=3,0;MQSB=0.999437;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,178:7:0 0,33,255:11:0 17 492 . G 0 . DP=35;I16=21,12,0,0,1252,48326,0,0,1926,113810,0,0,621,13859,0,0;QS=3,0;MQSB=0.988858;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,172:6:0 0,30,251:10:0 17 493 . A 0 . DP=34;I16=22,11,0,0,1273,49481,0,0,1926,113810,0,0,650,14672,0,0;QS=3,0;MQSB=0.981935;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,186:7:0 0,24,240:8:0 17 494 . A 0 . DP=34;I16=22,12,0,0,1326,52604,0,0,1986,117410,0,0,672,15182,0,0;QS=3,0;MQSB=0.986937;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,196:7:0 0,27,255:9:0 17 495 . G 0 . DP=34;I16=21,12,0,0,1255,48577,0,0,1926,113810,0,0,647,14611,0,0;QS=3,0;MQSB=0.988858;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,168:6:0 0,27,244:9:0 17 496 . A 0 . DP=34;I16=22,12,0,0,1250,46926,0,0,1986,117410,0,0,670,15220,0,0;QS=3,0;MQSB=0.986937;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,186:7:0 0,27,249:9:0 17 497 . C 0 . DP=34;I16=22,12,0,0,1250,47006,0,0,1986,117410,0,0,665,15087,0,0;QS=3,0;MQSB=0.986937;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,164:7:0 0,27,239:9:0 17 498 . A 0 . DP=34;I16=22,12,0,0,1286,49158,0,0,1986,117410,0,0,661,14987,0,0;QS=3,0;MQSB=0.986937;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,185:7:0 0,27,252:9:0 17 499 . T 0 . DP=34;I16=23,11,0,0,1224,45284,0,0,1986,117410,0,0,659,14919,0,0;QS=3,0;MQSB=0.979712;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,183:7:0 0,30,255:10:0 17 500 . A 0 . DP=34;I16=23,11,0,0,1230,45152,0,0,1986,117410,0,0,657,14833,0,0;QS=3,0;MQSB=0.979712;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,179:7:0 0,30,255:10:0 17 501 . T 0 . DP=33;I16=23,10,0,0,1241,47167,0,0,1926,113810,0,0,656,14778,0,0;QS=3,0;MQSB=0.972757;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,186:7:0 0,27,241:9:0 17 502 . G 0 . DP=33;I16=23,10,0,0,1215,45829,0,0,1926,113810,0,0,655,14753,0,0;QS=3,0;MQSB=0.972757;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,183:7:0 0,27,235:9:0 17 503 . T 0 . DP=34;I16=23,11,0,0,1194,43366,0,0,1986,117410,0,0,654,14758,0,0;QS=3,0;MQSB=0.979712;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,177:7:0 0,27,234:9:0 17 504 . C 0 . DP=34;I16=23,11,0,0,1218,45552,0,0,1986,117410,0,0,651,14643,0,0;QS=3,0;MQSB=0.979712;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,183:7:0 0,27,219:9:0 17 505 . C 0 . DP=35;I16=23,11,0,0,1207,44321,0,0,1986,117410,0,0,641,14509,0,0;QS=3,0;MQSB=0.979712;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,189:7:0 0,27,221:9:0 17 506 . A 0 . DP=35;I16=24,11,0,0,1266,46776,0,0,2046,121010,0,0,646,14504,0,0;QS=3,0;MQSB=0.977529;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,188:7:0 0,27,231:9:0 17 507 . C 0 . DP=35;I16=23,11,0,0,1220,45016,0,0,1986,117410,0,0,635,14401,0,0;QS=3,0;MQSB=0.979712;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,183:7:0 0,27,226:9:0 17 508 . A 0 . DP=34;I16=24,10,0,0,1204,44542,0,0,1986,117410,0,0,643,14491,0,0;QS=3,0;MQSB=0.970272;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,189:7:0 0,27,220:9:0 17 509 . C 0 . DP=34;I16=24,10,0,0,1272,48272,0,0,1986,117410,0,0,640,14430,0,0;QS=3,0;MQSB=0.970272;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,186:7:0 0,27,241:9:0 17 510 . A 0 . DP=34;I16=22,11,0,0,1194,44196,0,0,1926,113810,0,0,613,13773,0,0;QS=3,0;MQSB=0.981935;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,187:7:0 0,24,221:8:0 17 511 . A 0 . DP=34;I16=23,11,0,0,1222,45562,0,0,1986,117410,0,0,637,14395,0,0;QS=3,0;MQSB=0.979712;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,195:7:0 0,24,233:8:0 17 512 . A C, 0 . DP=33;I16=22,10,0,1,1121,40793,13,169,1866,110210,60,3600,628,14340,9,81;QS=2.97719,0.022807,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.981935;BQB=1;MQ0F=0 PL:DP:DV 0,39,255,51,255,255:18:1 0,21,183,21,183,183:7:0 0,24,231,24,231,231:8:0 17 513 . A 0 . DP=32;I16=20,10,0,0,1115,42183,0,0,1746,103010,0,0,598,13624,0,0;QS=3,0;MQSB=0.980594;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,175:6:0 0,24,233:8:0 17 514 . A T, 0 . DP=32;I16=20,9,0,1,1066,40004,16,256,1686,99410,60,3600,586,13500,11,121;QS=2.97075,0.0292505,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.980594;BQB=1;MQ0F=0 PL:DP:DV 0,31,255,45,255,255:16:1 0,18,171,18,171,171:6:0 0,24,235,24,235,235:8:0 17 515 . C 0 . DP=32;I16=18,10,0,0,1010,37294,0,0,1626,95810,0,0,561,12915,0,0;QS=3,0;MQSB=0.986018;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,167:6:0 0,24,211:8:0 17 516 . C 0 . DP=32;I16=21,10,0,0,1100,40570,0,0,1806,106610,0,0,612,13954,0,0;QS=3,0;MQSB=0.977926;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,187:7:0 0,24,215:8:0 17 517 . T 0 . DP=33;I16=23,10,0,0,1269,49995,0,0,1926,113810,0,0,636,14626,0,0;QS=3,0;MQSB=0.972757;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,201:7:0 0,24,242:8:0 17 518 . G 0 . DP=34;I16=24,10,0,0,1247,46839,0,0,1986,117410,0,0,636,14696,0,0;QS=3,0;MQSB=0.970272;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,182:7:0 0,24,220:8:0 17 519 . T 0 . DP=36;I16=25,11,0,0,1283,46693,0,0,2106,124610,0,0,636,14742,0,0;QS=3,0;MQSB=0.975394;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,21,177:7:0 0,24,224:8:0 17 520 . T 0 . DP=36;I16=24,11,0,0,1238,44894,0,0,2046,121010,0,0,613,14193,0,0;QS=3,0;MQSB=0.977529;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,21,180:7:0 0,24,223:8:0 17 521 . C 0 . DP=34;I16=25,9,0,0,1280,49454,0,0,1986,117410,0,0,641,14875,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,21,191:7:0 0,21,204:7:0 17 522 . A 0 . DP=32;I16=24,8,0,0,1158,43228,0,0,1897,112969,0,0,646,14960,0,0;QS=3,0;MQSB=0.872525;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,21,185:7:0 0,15,170:5:0 17 523 . T G, 0 . DP=32;I16=23,8,1,0,1184,45708,15,225,1837,109369,60,3600,626,14446,25,625;QS=2.9794,0.0206044,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.872525;BQB=1;MQ0F=0 PL:DP:DV 0,44,255,57,255,255:20:1 0,21,191,21,191,191:7:0 0,15,166,15,166,166:5:0 17 524 . T 0 . DP=32;I16=24,7,0,0,1084,39474,0,0,1837,109369,0,0,629,14483,0,0;QS=3,0;MQSB=0.851535;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,21,194:7:0 0,12,140:4:0 17 525 . G 0 . DP=32;I16=24,7,0,0,1181,45669,0,0,1837,109369,0,0,631,14495,0,0;QS=3,0;MQSB=0.851535;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,21,188:7:0 0,12,129:4:0 17 526 . C 0 . DP=32;I16=24,7,0,0,1146,43950,0,0,1860,111600,0,0,633,14531,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,21,185:7:0 0,12,131:4:0 17 527 . A 0 . DP=33;I16=24,8,0,0,1209,46265,0,0,1897,112969,0,0,636,14634,0,0;QS=3,0;MQSB=0.872525;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,194:7:0 0,18,181:6:0 17 528 . G 0 . DP=33;I16=24,8,0,0,1256,49824,0,0,1897,112969,0,0,634,14484,0,0;QS=3,0;MQSB=0.872525;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,18,169:6:0 0,18,193:6:0 17 529 . C 0 . DP=32;I16=24,7,0,0,1148,44362,0,0,1837,109369,0,0,633,14357,0,0;QS=3,0;MQSB=0.851535;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,187:7:0 0,18,184:6:0 17 530 . T 0 . DP=32;I16=25,7,0,0,1244,49168,0,0,1897,112969,0,0,657,14883,0,0;QS=3,0;MQSB=0.850154;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,196:7:0 0,18,202:6:0 17 531 . T 0 . DP=32;I16=25,7,0,0,1177,44171,0,0,1897,112969,0,0,654,14714,0,0;QS=3,0;MQSB=0.850154;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,181:7:0 0,18,193:6:0 17 532 . T 0 . DP=32;I16=24,7,0,0,1153,43543,0,0,1837,109369,0,0,630,14116,0,0;QS=3,0;MQSB=0.851535;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,181:7:0 0,18,192:6:0 17 533 . C 0 . DP=32;I16=24,7,0,0,1142,43940,0,0,1837,109369,0,0,619,13649,0,0;QS=3,0;MQSB=0.851535;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,178:7:0 0,18,180:6:0 17 534 . T 0 . DP=31;I16=24,6,0,0,1212,49426,0,0,1777,105769,0,0,615,13479,0,0;QS=3,0;MQSB=0.82403;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,205:7:0 0,18,205:6:0 17 535 . A 0 . DP=31;I16=24,6,0,0,1080,39870,0,0,1777,105769,0,0,611,13341,0,0;QS=3,0;MQSB=0.82403;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,194:7:0 0,18,189:6:0 17 536 . C 0 . DP=31;I16=24,7,0,0,1097,40707,0,0,1837,109369,0,0,631,13809,0,0;QS=3,0;MQSB=0.851535;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,184:7:0 0,18,157:6:0 17 537 . C 0 . DP=31;I16=22,7,0,0,1034,38564,0,0,1717,102169,0,0,587,12861,0,0;QS=3,0;MQSB=0.854582;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,172:7:0 0,18,183:6:0 17 538 . A 0 . DP=31;I16=24,7,0,0,1138,42478,0,0,1837,109369,0,0,620,13536,0,0;QS=3,0;MQSB=0.851535;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,189:7:0 0,18,181:6:0 17 539 . T 0 . DP=31;I16=24,7,0,0,1134,42070,0,0,1837,109369,0,0,614,13422,0,0;QS=3,0;MQSB=0.851535;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,178:7:0 0,18,181:6:0 17 540 . C 0 . DP=31;I16=24,7,0,0,1148,43768,0,0,1837,109369,0,0,608,13340,0,0;QS=3,0;MQSB=0.851535;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,177:7:0 0,18,178:6:0 17 541 . A 0 . DP=32;I16=24,6,0,0,1083,40483,0,0,1777,105769,0,0,551,11991,0,0;QS=3,0;MQSB=0.82403;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,180:8:0 0,15,150:5:0 17 542 . C 0 . DP=33;I16=25,6,0,0,1123,41759,0,0,1837,109369,0,0,570,12552,0,0;QS=3,0;MQSB=0.822578;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,172:8:0 0,18,174:6:0 17 543 . C 0 . DP=34;I16=25,9,0,0,1219,45959,0,0,1986,117410,0,0,601,13245,0,0;QS=3,0;MQSB=0.621145;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,27,194:9:0 0,18,188:6:0 17 544 . A 0 . DP=33;I16=24,8,0,0,1170,43898,0,0,1866,110210,0,0,570,12506,0,0;QS=3,0;MQSB=0.579578;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,24,192:8:0 0,18,180:6:0 17 545 . A 0 . DP=33;I16=25,8,0,0,1174,43602,0,0,1926,113810,0,0,587,12951,0,0;QS=3,0;MQSB=0.576102;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,24,190:8:0 0,18,184:6:0 17 546 . A 0 . DP=32;I16=24,8,0,0,1126,41444,0,0,1866,110210,0,0,580,12802,0,0;QS=3,0;MQSB=0.579578;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,166:7:0 0,18,193:6:0 17 547 . A 0 . DP=32;I16=24,7,0,0,1129,42381,0,0,1806,106610,0,0,547,12009,0,0;QS=3,0;MQSB=0.525788;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,180:7:0 0,18,195:6:0 17 548 . A 0 . DP=33;I16=23,9,0,0,1153,42673,0,0,1866,110210,0,0,561,12489,0,0;QS=3,0;MQSB=0.628357;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,181:7:0 0,21,211:7:0 17 549 . T G, 0 . DP=32;I16=22,8,0,1,1101,40987,20,400,1746,103010,60,3600,530,11716,25,625;QS=2.96748,0.0325203,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.632337;BQB=1;MQ0F=0 PL:DP:DV 0,31,255,48,255,255:17:1 0,21,168,21,168,168:7:0 0,21,208,21,208,208:7:0 17 550 . T 0 . DP=32;I16=22,9,0,0,1052,37298,0,0,1806,106610,0,0,548,12176,0,0;QS=3,0;MQSB=0.632337;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,150:7:0 0,21,220:7:0 17 551 . G 0 . DP=31;I16=22,9,0,0,1121,41639,0,0,1806,106610,0,0,541,12045,0,0;QS=3,0;MQSB=0.632337;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,172:7:0 0,21,208:7:0 17 552 . C 0 . DP=30;I16=21,9,0,0,1093,41365,0,0,1746,103010,0,0,535,11947,0,0;QS=3,0;MQSB=0.636601;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,167:7:0 0,21,208:7:0 17 553 . A 0 . DP=30;I16=20,8,0,0,981,35387,0,0,1626,95810,0,0,485,10831,0,0;QS=3,0;MQSB=0.596163;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,150:6:0 0,21,194:7:0 17 554 . A 0 . DP=30;I16=19,9,0,0,975,35601,0,0,1626,95810,0,0,488,10906,0,0;QS=3,0;MQSB=0.646113;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,139:5:0 0,24,211:8:0 17 555 . A 0 . DP=30;I16=20,10,0,0,1024,36526,0,0,1746,103010,0,0,514,11392,0,0;QS=3,0;MQSB=0.679025;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,150:6:0 0,21,211:7:0 17 556 . C 0 . DP=29;I16=20,9,0,0,1055,39195,0,0,1709,101641,0,0,509,11219,0,0;QS=3,0;MQSB=0.894839;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,158:6:0 0,18,186:6:0 17 557 . A 0 . DP=27;I16=18,9,0,0,987,36749,0,0,1589,94441,0,0,506,11074,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,157:6:0 0,18,186:6:0 17 558 . A 0 . DP=27;I16=18,8,0,0,948,35808,0,0,1560,93600,0,0,477,10281,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,143:5:0 0,18,177:6:0 17 559 . C A, 0 . DP=27;I16=17,8,0,1,908,33726,14,196,1500,90000,29,841,448,9516,25,625;QS=2.92708,0.0729167,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.90038;BQB=1;MQ0F=0 PL:DP:DV 0,42,255,42,255,255:14:0 0,4,116,15,119,123:6:1 0,18,169,18,169,169:6:0 17 560 . C 0 . DP=28;I16=19,9,0,0,992,36552,0,0,1649,98041,0,0,494,10654,0,0;QS=3,0;MQSB=0.896555;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,160:6:0 0,21,181:7:0 17 561 . A 0 . DP=28;I16=18,9,0,0,963,35455,0,0,1589,94441,0,0,466,9946,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,158:6:0 0,21,194:7:0 17 562 . C 0 . DP=28;I16=18,9,0,0,1006,38392,0,0,1589,94441,0,0,463,9893,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,153:6:0 0,21,187:7:0 17 563 . A 0 . DP=27;I16=17,9,0,0,893,32413,0,0,1529,90841,0,0,460,9820,0,0;QS=3,0;MQSB=0.90038;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,149:5:0 0,21,179:7:0 17 564 . C 0 . DP=27;I16=18,9,0,0,859,28747,0,0,1589,94441,0,0,482,10402,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,121:5:0 0,21,182:7:0 17 565 . G 0 . DP=30;I16=17,9,0,0,818,26928,0,0,1560,93600,0,0,454,9764,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,96:4:0 0,21,154:7:0 17 566 . C 0 . DP=29;I16=15,11,0,0,903,33405,0,0,1529,90841,0,0,424,9084,0,0;QS=3,0;MQSB=0.927041;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,155:5:0 0,18,167:6:0 17 567 . C 0 . DP=30;I16=15,12,0,0,932,33774,0,0,1589,94441,0,0,462,10178,0,0;QS=3,0;MQSB=0.935229;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,155:5:0 0,21,196:7:0 17 568 . C 0 . DP=29;I16=15,13,0,0,1057,40817,0,0,1649,98041,0,0,482,10438,0,0;QS=3,0;MQSB=0.942064;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,183:6:0 0,18,189:6:0 17 569 . T 0 . DP=30;I16=16,12,0,0,1056,41296,0,0,1649,98041,0,0,493,10655,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,190:6:0 0,21,213:7:0 17 570 . T 0 . DP=30;I16=16,12,0,0,954,34972,0,0,1680,100800,0,0,472,10086,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,157:5:0 0,21,195:7:0 17 571 . C 0 . DP=31;I16=17,12,0,0,1061,40675,0,0,1740,104400,0,0,472,10158,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,15,155:5:0 0,21,193:7:0 17 572 . A 0 . DP=31;I16=18,12,0,0,1102,42642,0,0,1769,105241,0,0,499,10887,0,0;QS=3,0;MQSB=0.929991;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,203:7:0 0,18,175:6:0 17 573 . A 0 . DP=31;I16=18,12,0,0,1057,38473,0,0,1769,105241,0,0,501,10975,0,0;QS=3,0;MQSB=0.929991;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,199:7:0 0,18,178:6:0 17 574 . C A, 0 . DP=31;I16=18,11,0,1,1088,41328,15,225,1740,104400,29,841,478,10422,25,625;QS=2.94071,0.0592885,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.929991;BQB=1;MQ0F=0 PL:DP:DV 0,48,255,48,255,255:16:0 0,9,170,21,173,177:8:1 0,18,173,18,173,173:6:0 17 575 . T 0 . DP=30;I16=16,10,0,0,1024,41260,0,0,1560,93600,0,0,448,9842,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,21,209:7:0 0,15,163:5:0 17 576 . G 0 . DP=30;I16=17,12,0,0,1047,40077,0,0,1709,101641,0,0,507,11087,0,0;QS=3,0;MQSB=0.931617;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,24,208:8:0 0,15,151:5:0 17 577 . G 0 . DP=30;I16=16,12,0,0,999,37747,0,0,1649,98041,0,0,489,10755,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,24,204:8:0 0,15,146:5:0 17 578 . G 0 . DP=29;I16=16,13,0,0,975,34803,0,0,1709,101641,0,0,520,11286,0,0;QS=3,0;MQSB=0.940317;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,24,198:8:0 0,15,151:5:0 17 579 . G 0 . DP=30;I16=15,13,0,0,1028,38752,0,0,1649,98041,0,0,484,10514,0,0;QS=3,0;MQSB=0.942064;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,24,224:8:0 0,12,145:4:0 17 580 . A C, 0 . DP=30;I16=15,14,1,0,1060,39178,16,256,1709,101641,60,3600,510,11078,17,289;QS=2.97338,0.0266223,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.946202;BQB=1;MQ0F=0 PL:DP:DV 0,34,255,48,255,255:17:1 0,24,221,24,221,221:8:0 0,15,155,15,155,155:5:0 17 581 . A 0 . DP=31;I16=16,15,0,0,1083,39215,0,0,1829,108841,0,0,530,11384,0,0;QS=3,0;MQSB=0.951229;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,24,223:8:0 0,15,153:5:0 17 582 . C 0 . DP=31;I16=15,15,0,0,1080,39870,0,0,1769,105241,0,0,519,11211,0,0;QS=3,0;MQSB=0.952765;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,207:8:0 0,15,151:5:0 17 583 . T 0 . DP=30;I16=16,14,0,0,1136,43996,0,0,1769,105241,0,0,539,11523,0,0;QS=3,0;MQSB=0.946202;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,238:8:0 0,15,163:5:0 17 584 . C 0 . DP=31;I16=16,13,0,0,1051,39351,0,0,1709,101641,0,0,499,10619,0,0;QS=3,0;MQSB=0.960189;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,207:7:0 0,15,157:5:0 17 585 . A 0 . DP=31;I16=17,14,0,0,1096,39866,0,0,1798,106082,0,0,549,11751,0,0;QS=3,0;MQSB=0.998229;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,24,211:8:0 0,15,157:5:0 17 586 . T 0 . DP=31;I16=16,14,0,0,1081,39839,0,0,1738,102482,0,0,546,11796,0,0;QS=3,0;MQSB=0.999136;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,212:8:0 0,15,156:5:0 17 587 . C 0 . DP=31;I16=17,13,0,0,1070,39402,0,0,1769,105241,0,0,532,11350,0,0;QS=3,0;MQSB=0.963674;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,201:7:0 0,15,155:5:0 17 588 . A 0 . DP=31;I16=17,14,0,0,1126,41642,0,0,1798,106082,0,0,562,12140,0,0;QS=3,0;MQSB=0.998229;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,24,222:8:0 0,15,164:5:0 17 589 . A 0 . DP=31;I16=17,14,0,0,1157,43973,0,0,1798,106082,0,0,568,12340,0,0;QS=3,0;MQSB=0.998229;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,24,236:8:0 0,15,165:5:0 17 590 . C 0 . DP=32;I16=16,14,0,0,1094,41302,0,0,1769,105241,0,0,549,11951,0,0;QS=3,0;MQSB=0.958545;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,192:6:0 0,18,175:6:0 17 591 . A 0 . DP=31;I16=16,15,0,0,1165,44163,0,0,1798,106082,0,0,579,12695,0,0;QS=3,0;MQSB=0.999805;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,209:7:0 0,18,188:6:0 17 592 . A 0 . DP=31;I16=15,15,0,0,1114,42144,0,0,1738,102482,0,0,571,12651,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,215:7:0 0,18,182:6:0 17 593 . C 0 . DP=31;I16=15,14,0,0,1065,39889,0,0,1709,101641,0,0,550,12132,0,0;QS=3,0;MQSB=0.954405;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,191:6:0 0,18,174:6:0 17 594 . A 0 . DP=33;I16=16,16,0,0,1163,42917,0,0,1858,109682,0,0,572,12672,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,205:7:0 0,24,212:8:0 17 595 . A 0 . DP=33;I16=17,16,0,0,1130,39996,0,0,1918,113282,0,0,589,12905,0,0;QS=3,0;MQSB=0.999838;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,198:7:0 0,24,213:8:0 17 596 . A 0 . DP=33;I16=16,16,0,0,1059,37039,0,0,1858,109682,0,0,590,12952,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,169:7:0 0,24,230:8:0 17 597 . C 0 . DP=33;I16=17,16,0,0,1196,44890,0,0,1918,113282,0,0,592,12990,0,0;QS=3,0;MQSB=0.999838;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,204:7:0 0,24,220:8:0 17 598 . T 0 . DP=33;I16=16,16,0,0,1214,47104,0,0,1858,109682,0,0,593,13013,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,217:7:0 0,24,239:8:0 17 599 . T 0 . DP=33;I16=16,17,0,0,1183,43669,0,0,1918,113282,0,0,599,13095,0,0;QS=3,0;MQSB=0.999838;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,197:7:0 0,27,247:9:0 17 600 . G 0 . DP=32;I16=15,17,0,0,1174,44066,0,0,1858,109682,0,0,601,13145,0,0;QS=3,0;MQSB=0.999287;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,194:7:0 0,24,232:8:0 17 601 . T 0 . DP=32;I16=15,17,0,0,1114,39954,0,0,1858,109682,0,0,603,13227,0,0;QS=3,0;MQSB=0.999287;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,188:7:0 0,24,235:8:0 17 602 . G 0 . DP=32;I16=15,15,0,0,1090,40326,0,0,1769,105241,0,0,555,12091,0,0;QS=3,0;MQSB=0.952765;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,185:6:0 0,24,229:8:0 17 603 . G 0 . DP=31;I16=15,16,0,0,1052,37460,0,0,1798,106082,0,0,607,13437,0,0;QS=3,0;MQSB=0.999805;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,186:7:0 0,24,219:8:0 17 604 . T 0 . DP=31;I16=14,16,0,0,1009,35893,0,0,1769,105241,0,0,564,12540,0,0;QS=3,0;MQSB=0.958545;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,24,195:8:0 0,21,212:7:0 17 605 . T 0 . DP=31;I16=12,15,0,0,1001,37741,0,0,1589,94441,0,0,514,11258,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,200:7:0 0,21,214:7:0 17 606 . T 0 . DP=31;I16=13,16,0,0,992,35202,0,0,1709,101641,0,0,587,13125,0,0;QS=3,0;MQSB=0.960189;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,21,189:7:0 0,24,217:8:0 17 607 . A 0 . DP=31;I16=14,16,0,0,1027,36129,0,0,1738,102482,0,0,607,13577,0,0;QS=3,0;MQSB=0.999136;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,176:7:0 0,24,222:8:0 17 608 . C 0 . DP=32;I16=15,15,0,0,983,33775,0,0,1769,105241,0,0,564,12564,0,0;QS=3,0;MQSB=0.952765;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,21,178:7:0 0,27,214:9:0 17 609 . C 0 . DP=32;I16=14,17,0,0,1074,38220,0,0,1798,106082,0,0,606,13678,0,0;QS=3,0;MQSB=0.998229;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,190:7:0 0,27,238:9:0 17 610 . C 0 . DP=32;I16=15,16,0,0,1158,44218,0,0,1829,108841,0,0,594,13444,0,0;QS=3,0;MQSB=0.951229;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,211:8:0 0,27,246:9:0 17 611 . A 0 . DP=32;I16=15,16,0,0,1080,39204,0,0,1798,106082,0,0,590,13260,0,0;QS=3,0;MQSB=0.999805;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,27,220:9:0 0,27,246:9:0 17 612 . C 0 . DP=33;I16=16,17,0,0,1175,43305,0,0,1918,113282,0,0,617,13993,0,0;QS=3,0;MQSB=0.999838;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,27,214:9:0 0,27,250:9:0 17 613 . A 0 . DP=34;I16=16,17,0,0,1131,40011,0,0,1949,116041,0,0,604,13874,0,0;QS=3,0;MQSB=0.954207;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,27,223:9:0 0,27,239:9:0 17 614 . C 0 . DP=34;I16=16,17,0,0,1183,43743,0,0,1949,116041,0,0,608,14022,0,0;QS=3,0;MQSB=0.954207;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,27,223:9:0 0,27,245:9:0 17 615 . A 0 . DP=34;I16=16,18,0,0,1199,43809,0,0,1978,116882,0,0,626,14394,0,0;QS=3,0;MQSB=0.999405;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,30,240:10:0 0,27,254:9:0 17 616 . A 0 . DP=34;I16=16,17,0,0,1190,44024,0,0,1949,116041,0,0,615,14351,0,0;QS=3,0;MQSB=0.954207;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,27,247:9:0 0,27,255:9:0 17 617 . T 0 . DP=33;I16=15,18,0,0,1170,43066,0,0,1918,113282,0,0,630,14624,0,0;QS=3,0;MQSB=0.998531;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,30,241:10:0 0,27,255:9:0 17 618 . G 0 . DP=34;I16=15,19,0,0,1166,41452,0,0,1978,116882,0,0,632,14706,0,0;QS=3,0;MQSB=0.997597;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,30,248:10:0 0,27,240:9:0 17 619 . G 0 . DP=32;I16=14,18,0,0,1153,42591,0,0,1858,109682,0,0,638,14816,0,0;QS=3,0;MQSB=0.997118;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,30,254:10:0 0,27,247:9:0 17 620 . A 0 . DP=32;I16=14,17,0,0,1083,39757,0,0,1798,106082,0,0,616,14176,0,0;QS=3,0;MQSB=0.998229;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,30,248:10:0 0,27,255:9:0 17 621 . A 0 . DP=32;I16=13,18,0,0,1170,45248,0,0,1798,106082,0,0,627,14519,0,0;QS=3,0;MQSB=0.995005;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,27,255:9:0 0,27,255:9:0 17 622 . G 0 . DP=32;I16=12,18,0,0,1060,39160,0,0,1769,105241,0,0,604,13888,0,0;QS=3,0;MQSB=0.968257;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,236:9:0 0,27,249:9:0 17 623 . A T, 0 . DP=32;I16=10,18,1,0,1010,37414,15,225,1649,98041,60,3600,563,12953,25,625;QS=2.96144,0.0385604,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.969907;BQB=1;MQ0F=0 PL:DP:DV 0,20,241,33,244,246:12:1 0,24,213,24,213,213:8:0 0,27,255,27,255,255:9:0 17 624 . C 0 . DP=32;I16=13,17,0,0,1034,37292,0,0,1738,102482,0,0,607,13887,0,0;QS=3,0;MQSB=0.996503;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,220:9:0 0,27,242:9:0 17 625 . C 0 . DP=32;I16=13,18,0,0,1108,41138,0,0,1798,106082,0,0,632,14470,0,0;QS=3,0;MQSB=0.995005;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,27,234:9:0 0,27,249:9:0 17 626 . A 0 . DP=32;I16=13,17,0,0,1090,40718,0,0,1738,102482,0,0,607,13827,0,0;QS=3,0;MQSB=0.996503;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,237:9:0 0,27,247:9:0 17 627 . C 0 . DP=32;I16=12,18,0,0,1146,44452,0,0,1769,105241,0,0,607,13833,0,0;QS=3,0;MQSB=0.968257;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,244:9:0 0,27,255:9:0 17 628 . T 0 . DP=32;I16=11,19,0,0,1124,43114,0,0,1769,105241,0,0,608,13862,0,0;QS=3,0;MQSB=0.972375;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,27,249:9:0 0,24,249:8:0 17 629 . T 0 . DP=32;I16=12,19,0,0,1114,41138,0,0,1798,106082,0,0,634,14490,0,0;QS=3,0;MQSB=0.989977;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,247:9:0 0,24,226:8:0 17 630 . A 0 . DP=31;I16=11,18,0,0,1101,42885,0,0,1740,104400,0,0,610,13844,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,24,242:8:0 0,24,235:8:0 17 631 . G 0 . DP=31;I16=12,18,0,0,1083,40525,0,0,1769,105241,0,0,636,14474,0,0;QS=3,0;MQSB=0.929991;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,233:8:0 0,24,223:8:0 17 632 . C 0 . DP=31;I16=11,18,0,0,1105,42665,0,0,1740,104400,0,0,612,13880,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,24,233:8:0 0,24,224:8:0 17 633 . A 0 . DP=31;I16=12,18,0,0,1056,38190,0,0,1769,105241,0,0,638,14562,0,0;QS=3,0;MQSB=0.929991;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,218:8:0 0,24,214:8:0 17 634 . A 0 . DP=31;I16=12,18,0,0,1110,42208,0,0,1769,105241,0,0,638,14594,0,0;QS=3,0;MQSB=0.929991;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,238:8:0 0,24,229:8:0 17 635 . C 0 . DP=31;I16=11,18,0,0,1031,37871,0,0,1740,104400,0,0,613,14025,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,24,220:8:0 0,24,229:8:0 17 636 . A 0 . DP=31;I16=11,18,0,0,1115,43327,0,0,1740,104400,0,0,611,14005,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,24,228:8:0 0,24,243:8:0 17 637 . A 0 . DP=31;I16=12,18,0,0,1152,44602,0,0,1769,105241,0,0,634,14634,0,0;QS=3,0;MQSB=0.929991;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,238:8:0 0,24,242:8:0 17 638 . A 0 . DP=31;I16=12,18,0,0,1118,42406,0,0,1769,105241,0,0,631,14611,0,0;QS=3,0;MQSB=0.929991;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,235:8:0 0,24,238:8:0 17 639 . A 0 . DP=30;I16=11,18,0,0,1037,38233,0,0,1709,101641,0,0,602,13934,0,0;QS=3,0;MQSB=0.921439;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,24,230:8:0 0,24,227:8:0 17 640 . A 0 . DP=31;I16=11,19,0,0,1154,45368,0,0,1769,105241,0,0,596,13804,0,0;QS=3,0;MQSB=0.91982;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,242:8:0 0,24,240:8:0 17 641 . G 0 . DP=31;I16=11,19,0,0,1018,36496,0,0,1769,105241,0,0,590,13650,0,0;QS=3,0;MQSB=0.91982;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,214:8:0 0,24,218:8:0 17 642 . G 0 . DP=30;I16=11,19,0,0,1057,38459,0,0,1769,105241,0,0,610,14148,0,0;QS=3,0;MQSB=0.91982;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,187:7:0 0,24,221:8:0 17 643 . A 0 . DP=29;I16=11,17,0,0,969,35477,0,0,1649,98041,0,0,585,13605,0,0;QS=3,0;MQSB=0.923174;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,199:7:0 0,24,237:8:0 17 644 . C A, 0 . DP=29;I16=9,18,1,0,898,30864,17,289,1620,97200,60,3600,549,12567,25,625;QS=2.95685,0.0431472,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0 PL:DP:DV 0,22,236,36,239,243:13:1 0,21,195,21,195,195:7:0 0,24,204,24,204,204:8:0 17 645 . C 0 . DP=30;I16=10,19,0,0,1067,40337,0,0,1709,101641,0,0,584,13274,0,0;QS=3,0;MQSB=0.909373;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,21,198:7:0 0,24,226:8:0 17 646 . A T, 0 . DP=31;I16=9,20,1,0,1044,38174,14,196,1740,104400,60,3600,553,12499,25,625;QS=2.97113,0.028866,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0 PL:DP:DV 0,27,255,39,255,255:14:1 0,21,197,21,197,197:7:0 0,27,229,27,229,229:9:0 17 647 . A 0 . DP=33;I16=10,21,0,0,1041,35883,0,0,1829,108841,0,0,573,12999,0,0;QS=3,0;MQSB=0.906252;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,21,189:7:0 0,30,213:10:0 17 648 . A 0 . DP=33;I16=11,22,0,0,1030,34122,0,0,1949,116041,0,0,594,13478,0,0;QS=3,0;MQSB=0.915545;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,188:7:0 0,30,194:10:0 17 649 . C 0 . DP=32;I16=10,21,0,0,1099,39879,0,0,1860,111600,0,0,566,12738,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,188:7:0 0,27,217:9:0 17 650 . T 0 . DP=31;I16=11,18,0,0,1006,37114,0,0,1709,101641,0,0,549,12407,0,0;QS=3,0;MQSB=0.921439;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,187:6:0 0,24,226:8:0 17 651 . C 0 . DP=32;I16=11,20,0,0,1117,41181,0,0,1829,108841,0,0,581,13107,0,0;QS=3,0;MQSB=0.918304;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,186:6:0 0,27,229:9:0 17 652 . C 0 . DP=32;I16=9,21,0,0,1126,43084,0,0,1769,105241,0,0,557,12423,0,0;QS=3,0;MQSB=0.893237;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,196:6:0 0,24,213:8:0 17 653 . T 0 . DP=33;I16=9,23,0,0,1141,42631,0,0,1889,112441,0,0,556,12382,0,0;QS=3,0;MQSB=0.890331;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,198:6:0 0,24,225:8:0 17 654 . G 0 . DP=33;I16=10,23,0,0,1171,43025,0,0,1949,116041,0,0,578,12798,0,0;QS=3,0;MQSB=0.903508;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,18,191:6:0 0,24,223:8:0 17 655 . G 0 . DP=32;I16=10,22,0,0,1118,40202,0,0,1889,112441,0,0,575,12573,0,0;QS=3,0;MQSB=0.904837;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,181:6:0 0,24,213:8:0 17 656 . T 0 . DP=32;I16=10,22,0,0,1090,38566,0,0,1889,112441,0,0,571,12333,0,0;QS=3,0;MQSB=0.904837;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,185:6:0 0,24,213:8:0 17 657 . A 0 . DP=32;I16=10,21,0,0,1100,40006,0,0,1829,108841,0,0,557,12029,0,0;QS=3,0;MQSB=0.906252;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,183:6:0 0,24,191:8:0 17 658 . C 0 . DP=32;I16=10,21,0,0,1115,41039,0,0,1829,108841,0,0,542,11520,0,0;QS=3,0;MQSB=0.906252;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,15,168:5:0 0,24,211:8:0 17 659 . A 0 . DP=32;I16=10,21,0,0,1141,42897,0,0,1829,108841,0,0,547,11685,0,0;QS=3,0;MQSB=0.906252;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,191:6:0 0,24,216:8:0 17 660 . T 0 . DP=33;I16=10,22,0,0,1139,41887,0,0,1889,112441,0,0,553,11659,0,0;QS=3,0;MQSB=0.904837;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,186:6:0 0,24,214:8:0 17 661 . G 0 . DP=32;I16=9,22,0,0,1107,41531,0,0,1829,108841,0,0,549,11549,0,0;QS=3,0;MQSB=0.891738;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,191:6:0 0,21,188:7:0 17 662 . C 0 . DP=32;I16=8,22,0,0,1087,40811,0,0,1800,108000,0,0,523,10991,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,192:6:0 0,21,187:7:0 17 663 . A 0 . DP=32;I16=9,22,0,0,1036,36816,0,0,1829,108841,0,0,540,11388,0,0;QS=3,0;MQSB=0.891738;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,192:6:0 0,21,186:7:0 17 664 . A 0 . DP=32;I16=9,23,0,0,1125,40759,0,0,1889,112441,0,0,552,11628,0,0;QS=3,0;MQSB=0.890331;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,18,188:6:0 0,21,184:7:0 17 665 . C 0 . DP=30;I16=9,21,0,0,1075,39697,0,0,1769,105241,0,0,550,11650,0,0;QS=3,0;MQSB=0.893237;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,186:6:0 0,21,184:7:0 17 666 . T 0 . DP=29;I16=9,20,0,0,1096,41946,0,0,1709,101641,0,0,547,11607,0,0;QS=3,0;MQSB=0.894839;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,15,171:5:0 0,21,194:7:0 17 667 . G 0 . DP=31;I16=11,19,0,0,1037,37627,0,0,1769,105241,0,0,524,11198,0,0;QS=3,0;MQSB=0.91982;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,189:6:0 0,21,188:7:0 17 668 . A 0 . DP=31;I16=11,20,0,0,1102,39980,0,0,1829,108841,0,0,543,11625,0,0;QS=3,0;MQSB=0.918304;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,190:6:0 0,21,187:7:0 17 669 . C 0 . DP=30;I16=10,19,0,0,1051,38869,0,0,1740,104400,0,0,528,11464,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,182:6:0 0,21,189:7:0 17 670 . A 0 . DP=30;I16=11,19,0,0,1121,43423,0,0,1769,105241,0,0,540,11642,0,0;QS=3,0;MQSB=0.91982;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,194:6:0 0,21,205:7:0 17 671 . G 0 . DP=30;I16=11,19,0,0,1101,41035,0,0,1769,105241,0,0,537,11637,0,0;QS=3,0;MQSB=0.91982;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,181:6:0 0,21,191:7:0 17 672 . A 0 . DP=30;I16=10,19,0,0,1031,37795,0,0,1740,104400,0,0,521,11479,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,185:6:0 0,21,185:7:0 17 673 . T 0 . DP=29;I16=8,19,0,0,954,34984,0,0,1589,94441,0,0,497,10885,0,0;QS=3,0;MQSB=0.880529;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,126:4:0 0,21,192:7:0 17 674 . G 0 . DP=29;I16=10,18,0,0,974,35736,0,0,1649,98041,0,0,497,10827,0,0;QS=3,0;MQSB=0.911099;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,146:5:0 0,21,185:7:0 17 675 . A 0 . DP=28;I16=9,19,0,0,996,36338,0,0,1649,98041,0,0,517,11389,0,0;QS=3,0;MQSB=0.896555;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,124:4:0 0,21,189:7:0 17 676 . A 0 . DP=29;I16=8,19,0,0,988,36578,0,0,1589,94441,0,0,462,10106,0,0;QS=3,0;MQSB=0.880529;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,128:4:0 0,21,186:7:0 17 677 . T 0 . DP=29;I16=9,20,0,0,1006,36126,0,0,1709,101641,0,0,507,11303,0,0;QS=3,0;MQSB=0.894839;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,12,120:4:0 0,21,192:7:0 17 678 . C 0 . DP=29;I16=9,20,0,0,1020,36984,0,0,1709,101641,0,0,502,11280,0,0;QS=3,0;MQSB=0.894839;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,12,117:4:0 0,21,191:7:0 17 679 . T 0 . DP=27;I16=6,19,0,0,902,33612,0,0,1469,87241,0,0,460,10414,0,0;QS=3,0;MQSB=0.833024;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,96:3:0 0,21,195:7:0 17 680 . C 0 . DP=26;I16=7,18,0,0,879,32295,0,0,1469,87241,0,0,468,10482,0,0;QS=3,0;MQSB=0.862128;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,98:3:0 0,21,181:7:0 17 681 . A 0 . DP=27;I16=7,17,0,0,844,30190,0,0,1409,83641,0,0,465,10425,0,0;QS=3,0;MQSB=0.864405;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,106:3:0 0,21,174:7:0 17 682 . A 0 . DP=27;I16=7,20,0,0,919,32179,0,0,1589,94441,0,0,500,11096,0,0;QS=3,0;MQSB=0.858077;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,145:5:0 0,21,176:7:0 17 683 . A 0 . DP=29;I16=8,21,0,0,949,32735,0,0,1709,101641,0,0,499,11103,0,0;QS=3,0;MQSB=0.876998;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,172:6:0 0,21,167:7:0 17 684 . C 0 . DP=29;I16=6,21,0,0,802,24468,0,0,1589,94441,0,0,473,10459,0,0;QS=3,0;MQSB=0.829029;MQ0F=0 PL:DP:DV 0,45,251:15:0 0,15,118:5:0 0,21,145:7:0 17 685 . G 0 . DP=28;I16=6,21,0,0,908,32092,0,0,1620,97200,0,0,498,11090,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,130:5:0 0,21,175:7:0 17 686 . C 0 . DP=28;I16=7,21,0,0,977,35493,0,0,1680,100800,0,0,500,11080,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,170:6:0 0,21,180:7:0 17 687 . A 0 . DP=28;I16=6,20,0,0,900,31952,0,0,1560,93600,0,0,475,10469,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,175:6:0 0,21,174:7:0 17 688 . T 0 . DP=27;I16=6,21,0,0,937,33971,0,0,1620,97200,0,0,501,11135,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,153:6:0 0,21,187:7:0 17 689 . T A, 0 . DP=27;I16=5,20,1,0,829,28967,13,169,1500,90000,60,3600,463,10359,25,625;QS=2.97105,0.0289532,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0 PL:DP:DV 0,28,231,39,234,234:14:1 0,15,116,15,116,116:5:0 0,21,185,21,185,185:7:0 17 690 . C 0 . DP=27;I16=6,20,0,0,935,34553,0,0,1560,93600,0,0,486,10974,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,146:5:0 0,21,165:7:0 17 691 . C 0 . DP=26;I16=5,18,0,0,842,31906,0,0,1380,82800,0,0,446,10166,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,129:4:0 0,18,161:6:0 17 692 . T 0 . DP=26;I16=6,19,0,0,886,32434,0,0,1500,90000,0,0,486,11082,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,146:5:0 0,18,164:6:0 17 693 . C 0 . DP=27;I16=6,20,0,0,891,31839,0,0,1560,93600,0,0,483,11007,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,141:5:0 0,21,172:7:0 17 694 . C 0 . DP=27;I16=6,21,0,0,808,25004,0,0,1620,97200,0,0,498,11248,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,242:14:0 0,18,136:6:0 0,21,146:7:0 17 695 . G 0 . DP=25;I16=4,20,0,0,807,28037,0,0,1440,86400,0,0,476,10692,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,246:13:0 0,18,145:6:0 0,15,124:5:0 17 696 . T 0 . DP=25;I16=5,20,0,0,896,32870,0,0,1500,90000,0,0,499,11129,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,150:6:0 0,15,141:5:0 17 697 . G 0 . DP=26;I16=5,19,0,0,852,30594,0,0,1409,83641,0,0,450,9858,0,0;QS=3,0;MQSB=0.796124;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,142:5:0 0,15,120:5:0 17 698 . T 0 . DP=27;I16=6,20,0,0,917,33201,0,0,1529,90841,0,0,502,11114,0,0;QS=3,0;MQSB=0.83095;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,154:6:0 0,15,131:5:0 17 699 . G 0 . DP=28;I16=5,21,0,0,923,34103,0,0,1529,90841,0,0,498,10884,0,0;QS=3,0;MQSB=0.79189;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,163:6:0 0,15,132:5:0 17 700 . A 0 . DP=28;I16=6,22,0,0,989,35833,0,0,1618,95282,0,0,530,11626,0,0;QS=3,0;MQSB=0.904554;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,169:7:0 0,18,148:6:0 17 701 . A 0 . DP=29;I16=5,23,0,0,992,35956,0,0,1618,95282,0,0,517,11459,0,0;QS=3,0;MQSB=0.864394;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,186:7:0 0,18,144:6:0 17 702 . A 0 . DP=29;I16=5,23,0,0,1103,44105,0,0,1618,95282,0,0,520,11508,0,0;QS=3,0;MQSB=0.864394;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,204:7:0 0,18,159:6:0 17 703 . G 0 . DP=29;I16=5,23,0,0,1014,37508,0,0,1618,95282,0,0,522,11534,0,0;QS=3,0;MQSB=0.864394;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,172:7:0 0,18,144:6:0 17 704 . A T, 0 . DP=29;I16=4,23,1,0,954,34200,16,256,1558,91682,60,3600,499,10963,13,169;QS=2.97064,0.0293578,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.864394;BQB=1;MQ0F=0 PL:DP:DV 0,31,255,45,255,255:16:1 0,18,154,18,154,154:6:0 0,18,139,18,139,139:6:0 17 705 . A 0 . DP=29;I16=6,22,0,0,1042,39930,0,0,1618,95282,0,0,513,11189,0,0;QS=3,0;MQSB=0.904554;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,189:7:0 0,18,157:6:0 17 706 . G 0 . DP=30;I16=6,23,0,0,1029,37763,0,0,1678,98882,0,0,513,11225,0,0;QS=3,0;MQSB=0.900586;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,170:7:0 0,18,131:6:0 17 707 . C 0 . DP=30;I16=5,22,0,0,944,34494,0,0,1558,91682,0,0,464,10040,0,0;QS=3,0;MQSB=0.868709;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,131:5:0 0,18,134:6:0 17 708 . C 0 . DP=30;I16=6,24,0,0,898,27574,0,0,1738,102482,0,0,540,12010,0,0;QS=3,0;MQSB=0.896846;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,24,153:8:0 0,18,110:6:0 17 709 . G 0 . DP=29;I16=5,22,0,0,968,36130,0,0,1558,91682,0,0,507,11343,0,0;QS=3,0;MQSB=0.868709;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,178:8:0 0,15,132:5:0 17 710 . G 0 . DP=29;I16=5,23,0,0,961,34825,0,0,1618,95282,0,0,533,12029,0,0;QS=3,0;MQSB=0.864394;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,24,174:8:0 0,15,122:5:0 17 711 . A 0 . DP=29;I16=6,23,0,0,996,35700,0,0,1647,96123,0,0,565,12723,0,0;QS=3,0;MQSB=0.957107;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,171:7:0 0,18,142:6:0 17 712 . C 0 . DP=29;I16=6,23,0,0,1069,40863,0,0,1647,96123,0,0,565,12767,0,0;QS=3,0;MQSB=0.957107;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,156:7:0 0,18,146:6:0 17 713 . T 0 . DP=29;I16=6,23,0,0,1072,40426,0,0,1647,96123,0,0,565,12835,0,0;QS=3,0;MQSB=0.957107;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,187:7:0 0,18,149:6:0 17 714 . C 0 . DP=28;I16=6,21,0,0,988,37236,0,0,1558,91682,0,0,541,12301,0,0;QS=3,0;MQSB=0.90877;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,153:6:0 0,15,125:5:0 17 715 . A 0 . DP=28;I16=6,20,0,0,884,31414,0,0,1498,88082,0,0,522,12004,0,0;QS=3,0;MQSB=0.913254;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,138:6:0 0,15,120:5:0 17 716 . C 0 . DP=29;I16=4,23,0,0,998,37756,0,0,1527,88923,0,0,547,12501,0,0;QS=3,0;MQSB=0.877203;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,141:5:0 0,18,145:6:0 17 717 . A 0 . DP=31;I16=8,23,0,0,1136,42928,0,0,1767,103323,0,0,574,13254,0,0;QS=3,0;MQSB=0.987595;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,212:8:0 0,18,153:6:0 17 718 . G 0 . DP=29;I16=7,22,0,0,1066,40006,0,0,1647,96123,0,0,579,13407,0,0;QS=3,0;MQSB=0.979435;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,24,223:8:0 0,18,139:6:0 17 719 . G 0 . DP=29;I16=7,19,0,0,952,35868,0,0,1529,90841,0,0,510,11756,0,0;QS=3,0;MQSB=0.860025;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,204:8:0 0,12,120:4:0 17 720 . G 0 . DP=28;I16=6,20,0,0,986,37838,0,0,1467,85323,0,0,552,12940,0,0;QS=3,0;MQSB=0.970805;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,24,216:8:0 0,18,147:6:0 17 721 . C 0 . DP=28;I16=6,21,0,0,989,36901,0,0,1527,88923,0,0,567,13183,0,0;QS=3,0;MQSB=0.966147;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,24,197:8:0 0,18,147:6:0 17 722 . A 0 . DP=28;I16=6,21,0,0,949,33837,0,0,1527,88923,0,0,569,13225,0,0;QS=3,0;MQSB=0.966147;MQ0F=0 PL:DP:DV 0,39,244:13:0 0,24,205:8:0 0,18,143:6:0 17 723 . A 0 . DP=28;I16=6,19,0,0,925,34825,0,0,1438,84482,0,0,530,12366,0,0;QS=3,0;MQSB=0.918029;MQ0F=0 PL:DP:DV 0,36,253:12:0 0,24,206:8:0 0,15,135:5:0 17 724 . C 0 . DP=28;I16=5,22,0,0,967,35983,0,0,1527,88923,0,0,566,13028,0,0;QS=3,0;MQSB=0.932273;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,21,161:7:0 0,18,140:6:0 17 725 . A 0 . DP=28;I16=6,21,0,0,911,31971,0,0,1527,88923,0,0,565,12987,0,0;QS=3,0;MQSB=0.966147;MQ0F=0 PL:DP:DV 0,42,246:14:0 0,21,168:7:0 0,18,137:6:0 17 726 . C 0 . DP=28;I16=6,21,0,0,947,34531,0,0,1527,88923,0,0,563,12919,0,0;QS=3,0;MQSB=0.966147;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,21,166:7:0 0,18,136:6:0 17 727 . A C, 0 . DP=28;I16=6,20,0,1,904,32194,17,289,1498,88082,29,841,535,12199,25,625;QS=2.90811,0.0918919,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.966147;BQB=1;MQ0F=0 PL:DP:DV 0,42,247,42,247,247:14:0 0,21,191,21,191,191:7:0 0,1,109,15,112,119:6:1 17 728 . C 0 . DP=29;I16=6,22,0,0,1018,37990,0,0,1587,92523,0,0,557,12701,0,0;QS=3,0;MQSB=0.961573;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,208:8:0 0,18,142:6:0 17 729 . T 0 . DP=29;I16=7,22,0,0,1063,39315,0,0,1647,96123,0,0,581,13227,0,0;QS=3,0;MQSB=0.979435;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,244:9:0 0,18,142:6:0 17 730 . A 0 . DP=29;I16=7,21,0,0,993,35883,0,0,1618,95282,0,0,555,12529,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,219:9:0 0,15,135:5:0 17 731 . T 0 . DP=29;I16=7,22,0,0,1024,37312,0,0,1647,96123,0,0,578,13058,0,0;QS=3,0;MQSB=0.979435;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,210:9:0 0,18,148:6:0 17 732 . C 0 . DP=29;I16=7,21,0,0,1006,37058,0,0,1618,95282,0,0,550,12314,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,219:9:0 0,15,129:5:0 17 733 . T 0 . DP=29;I16=7,21,0,0,985,35497,0,0,1618,95282,0,0,547,12221,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,222:9:0 0,15,134:5:0 17 734 . G 0 . DP=29;I16=7,21,0,0,997,36453,0,0,1587,92523,0,0,544,12154,0,0;QS=3,0;MQSB=0.982906;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,204:8:0 0,18,139:6:0 17 735 . A 0 . DP=30;I16=7,21,0,0,963,33993,0,0,1618,95282,0,0,515,11437,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,190:8:0 0,18,148:6:0 17 736 . C 0 . DP=30;I16=8,20,0,0,1042,39326,0,0,1618,95282,0,0,512,11320,0,0;QS=3,0;MQSB=0.954515;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,218:8:0 0,18,152:6:0 17 737 . T 0 . DP=30;I16=8,22,0,0,1071,39825,0,0,1707,99723,0,0,560,12480,0,0;QS=3,0;MQSB=0.990151;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,243:9:0 0,21,149:7:0 17 738 . G 0 . DP=30;I16=8,21,0,0,1016,36816,0,0,1678,98882,0,0,533,11793,0,0;QS=3,0;MQSB=0.950946;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,210:9:0 0,18,141:6:0 17 739 . T 0 . DP=30;I16=7,22,0,0,1020,37326,0,0,1647,96123,0,0,534,11900,0,0;QS=3,0;MQSB=0.979435;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,202:8:0 0,21,155:7:0 17 740 . T 0 . DP=29;I16=7,21,0,0,1011,37465,0,0,1587,92523,0,0,532,11848,0,0;QS=3,0;MQSB=0.982906;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,24,232:8:0 0,21,155:7:0 17 741 . T 0 . DP=29;I16=7,21,0,0,984,36052,0,0,1587,92523,0,0,528,11722,0,0;QS=3,0;MQSB=0.982906;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,24,224:8:0 0,21,151:7:0 17 742 . C 0 . DP=30;I16=8,21,0,0,1046,39056,0,0,1678,98882,0,0,525,11671,0,0;QS=3,0;MQSB=0.950946;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,243:9:0 0,18,136:6:0 17 743 . A 0 . DP=30;I16=8,21,0,0,1026,37156,0,0,1678,98882,0,0,520,11500,0,0;QS=3,0;MQSB=0.950946;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,239:9:0 0,18,131:6:0 17 744 . T 0 . DP=30;I16=8,22,0,0,1100,41010,0,0,1707,99723,0,0,540,11984,0,0;QS=3,0;MQSB=0.990151;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,247:9:0 0,21,154:7:0 17 745 . G 0 . DP=31;I16=9,22,0,0,1078,38890,0,0,1767,103323,0,0,535,11873,0,0;QS=3,0;MQSB=0.996219;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,227:9:0 0,24,178:8:0 17 746 . G 0 . DP=31;I16=9,21,0,0,1001,35191,0,0,1707,99723,0,0,531,11793,0,0;QS=3,0;MQSB=0.997698;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,27,201:9:0 0,24,178:8:0 17 747 . G 0 . DP=30;I16=9,20,0,0,1017,36831,0,0,1647,96123,0,0,509,11343,0,0;QS=3,0;MQSB=0.99889;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,201:8:0 0,21,174:7:0 17 748 . A 0 . DP=30;I16=10,20,0,0,1046,37438,0,0,1707,99723,0,0,529,11721,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,27,242:9:0 0,18,159:6:0 17 749 . A 0 . DP=31;I16=10,21,0,0,1077,38303,0,0,1767,103323,0,0,530,11728,0,0;QS=3,0;MQSB=0.999777;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,27,240:9:0 0,18,164:6:0 17 750 . A 0 . DP=32;I16=10,20,0,0,1129,43093,0,0,1738,102482,0,0,500,11252,0,0;QS=3,0;MQSB=0.976097;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,232:8:0 0,15,161:5:0 17 751 . G 0 . DP=31;I16=11,20,0,0,1065,37955,0,0,1767,103323,0,0,535,11787,0,0;QS=3,0;MQSB=0.999148;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,27,206:9:0 0,18,150:6:0 17 752 . T 0 . DP=31;I16=11,20,0,0,1034,35786,0,0,1767,103323,0,0,537,11793,0,0;QS=3,0;MQSB=0.999148;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,27,223:9:0 0,18,143:6:0 17 753 . C 0 . DP=31;I16=12,19,0,0,1073,38779,0,0,1767,103323,0,0,540,11834,0,0;QS=3,0;MQSB=0.994873;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,30,227:10:0 0,18,158:6:0 17 754 . T 0 . DP=31;I16=12,19,0,0,1135,42527,0,0,1767,103323,0,0,542,11808,0,0;QS=3,0;MQSB=0.994873;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,30,255:10:0 0,18,172:6:0 17 755 . G 0 . DP=31;I16=12,19,0,0,1157,43695,0,0,1767,103323,0,0,544,11814,0,0;QS=3,0;MQSB=0.994873;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,30,255:10:0 0,18,162:6:0 17 756 . G 0 . DP=30;I16=12,17,0,0,1002,35566,0,0,1647,96123,0,0,539,11753,0,0;QS=3,0;MQSB=0.988062;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,30,242:10:0 0,18,157:6:0 17 757 . A 0 . DP=30;I16=12,17,0,0,1035,37723,0,0,1678,98882,0,0,523,11197,0,0;QS=3,0;MQSB=0.993891;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,30,248:10:0 0,15,152:5:0 17 758 . A 0 . DP=30;I16=12,18,0,0,1015,35685,0,0,1707,99723,0,0,549,11825,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,30,247:10:0 0,18,156:6:0 17 759 . A 0 . DP=30;I16=12,16,0,0,998,36498,0,0,1618,95282,0,0,526,11472,0,0;QS=3,0;MQSB=0.995699;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,242:9:0 0,15,151:5:0 17 760 . C 0 . DP=31;I16=12,18,0,0,916,29466,0,0,1707,99723,0,0,547,11741,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,30,217:10:0 0,18,128:6:0 17 761 . G 0 . DP=30;I16=11,16,0,0,932,32884,0,0,1589,94441,0,0,512,11028,0,0;QS=3,0;MQSB=0.925036;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,27,220:9:0 0,15,153:5:0 17 762 . G 0 . DP=29;I16=11,18,0,0,1012,36984,0,0,1647,96123,0,0,541,11629,0,0;QS=3,0;MQSB=0.995968;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,27,220:9:0 0,21,173:7:0 17 763 . C A, 0 . DP=29;I16=11,16,0,1,948,35124,15,225,1558,91682,29,841,527,11473,2,4;QS=2.93697,0.0630252,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.993109;BQB=1;MQ0F=0 PL:DP:DV 0,39,255,39,255,255:13:0 0,24,193,24,193,193:8:0 0,6,158,18,161,165:7:1 17 764 . A 0 . DP=31;I16=12,18,0,0,1051,37737,0,0,1738,102482,0,0,516,11020,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,30,250:10:0 0,18,163:6:0 17 765 . A 0 . DP=32;I16=12,18,0,0,1071,39369,0,0,1738,102482,0,0,525,11379,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,30,254:10:0 0,18,169:6:0 17 766 . C 0 . DP=31;I16=13,18,0,0,1065,38285,0,0,1798,106082,0,0,547,11797,0,0;QS=3,0;MQSB=0.995005;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,33,235:11:0 0,18,164:6:0 17 767 . A 0 . DP=30;I16=12,18,0,0,996,34692,0,0,1738,102482,0,0,552,11926,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,33,244:11:0 0,18,148:6:0 17 768 . C 0 . DP=30;I16=12,18,0,0,1095,40739,0,0,1738,102482,0,0,556,12038,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,33,255:11:0 0,18,173:6:0 17 769 . C 0 . DP=30;I16=12,18,0,0,1110,42272,0,0,1738,102482,0,0,559,12133,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,33,255:11:0 0,18,173:6:0 17 770 . A 0 . DP=30;I16=12,18,0,0,1098,40852,0,0,1738,102482,0,0,562,12262,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,33,255:11:0 0,18,171:6:0 17 771 . T 0 . DP=30;I16=12,18,0,0,1111,41771,0,0,1738,102482,0,0,563,12325,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,33,255:11:0 0,18,177:6:0 17 772 . T 0 . DP=30;I16=12,18,0,0,1107,41429,0,0,1738,102482,0,0,563,12373,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,33,255:11:0 0,18,176:6:0 17 773 . G 0 . DP=30;I16=12,18,0,0,1123,42761,0,0,1738,102482,0,0,562,12406,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,33,255:11:0 0,18,175:6:0 17 774 . A 0 . DP=30;I16=12,18,0,0,1151,44801,0,0,1738,102482,0,0,560,12422,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,33,255:11:0 0,18,179:6:0 17 775 . G 0 . DP=30;I16=12,18,0,0,1136,43766,0,0,1738,102482,0,0,557,12419,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,33,255:11:0 0,18,177:6:0 17 776 . A 0 . DP=29;I16=12,17,0,0,1053,38865,0,0,1678,98882,0,0,553,12345,0,0;QS=3,0;MQSB=0.993891;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,30,255:10:0 0,18,169:6:0 17 777 . C 0 . DP=28;I16=12,16,0,0,1019,37631,0,0,1618,95282,0,0,550,12298,0,0;QS=3,0;MQSB=0.995699;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,27,229:9:0 0,18,165:6:0 17 778 . A 0 . DP=28;I16=12,16,0,0,1079,42041,0,0,1618,95282,0,0,547,12277,0,0;QS=3,0;MQSB=0.995699;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,27,241:9:0 0,18,181:6:0 17 779 . G 0 . DP=28;I16=12,16,0,0,1025,38825,0,0,1618,95282,0,0,543,12231,0,0;QS=3,0;MQSB=0.995699;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,27,231:9:0 0,18,170:6:0 17 780 . A 0 . DP=28;I16=12,16,0,0,1005,36773,0,0,1618,95282,0,0,538,12160,0,0;QS=3,0;MQSB=0.995699;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,27,242:9:0 0,18,166:6:0 17 781 . A 0 . DP=28;I16=12,15,0,0,965,35857,0,0,1589,94441,0,0,519,11889,0,0;QS=3,0;MQSB=0.935229;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,222:8:0 0,15,159:5:0 17 782 . A 0 . DP=28;I16=12,16,0,0,1003,37315,0,0,1618,95282,0,0,530,12044,0,0;QS=3,0;MQSB=0.995699;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,229:8:0 0,18,171:6:0 17 783 . A 0 . DP=28;I16=12,16,0,0,989,36477,0,0,1618,95282,0,0,527,12001,0,0;QS=3,0;MQSB=0.995699;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,24,230:8:0 0,21,178:7:0 17 784 . C 0 . DP=26;I16=11,15,0,0,967,36387,0,0,1498,88082,0,0,527,11983,0,0;QS=3,0;MQSB=0.994627;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,207:7:0 0,21,178:7:0 17 785 . A 0 . DP=26;I16=11,15,0,0,989,38499,0,0,1498,88082,0,0,527,11989,0,0;QS=3,0;MQSB=0.994627;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,213:7:0 0,21,187:7:0 17 786 . G 0 . DP=26;I16=11,14,0,0,938,35698,0,0,1438,84482,0,0,501,11343,0,0;QS=3,0;MQSB=0.996634;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,21,211:7:0 0,21,180:7:0 17 787 . G 0 . DP=26;I16=11,15,0,0,914,33428,0,0,1498,88082,0,0,525,11969,0,0;QS=3,0;MQSB=0.994627;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,189:7:0 0,21,175:7:0 17 788 . T 0 . DP=26;I16=11,15,0,0,913,33357,0,0,1498,88082,0,0,524,11992,0,0;QS=3,0;MQSB=0.994627;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,180:7:0 0,21,188:7:0 17 789 . G 0 . DP=26;I16=11,15,0,0,963,36645,0,0,1498,88082,0,0,523,12037,0,0;QS=3,0;MQSB=0.994627;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,193:7:0 0,21,183:7:0 17 790 . A 0 . DP=26;I16=11,15,0,0,999,39113,0,0,1498,88082,0,0,520,12002,0,0;QS=3,0;MQSB=0.994627;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,198:7:0 0,21,201:7:0 17 791 . G 0 . DP=26;I16=11,15,0,0,934,34208,0,0,1498,88082,0,0,516,11934,0,0;QS=3,0;MQSB=0.994627;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,180:7:0 0,21,172:7:0 17 792 . T 0 . DP=26;I16=11,15,0,0,895,32009,0,0,1498,88082,0,0,511,11833,0,0;QS=3,0;MQSB=0.994627;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,184:7:0 0,21,172:7:0 17 793 . G 0 . DP=27;I16=11,15,0,0,965,36389,0,0,1498,88082,0,0,504,11652,0,0;QS=3,0;MQSB=0.994627;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,193:7:0 0,21,170:7:0 17 794 . G 0 . DP=26;I16=11,15,0,0,888,31804,0,0,1498,88082,0,0,508,11592,0,0;QS=3,0;MQSB=0.994627;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,24,198:8:0 0,18,161:6:0 17 795 . T 0 . DP=26;I16=9,15,0,0,859,31399,0,0,1409,83641,0,0,472,10908,0,0;QS=3,0;MQSB=0.96464;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,21,211:7:0 0,18,160:6:0 17 796 . T 0 . DP=27;I16=11,16,0,0,907,31641,0,0,1558,91682,0,0,499,11375,0,0;QS=3,0;MQSB=0.992419;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,27,221:9:0 0,15,150:5:0 17 797 . G 0 . DP=26;I16=11,15,0,0,882,31700,0,0,1529,90841,0,0,498,11298,0,0;QS=3,0;MQSB=0.927041;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,27,215:9:0 0,12,135:4:0 17 798 . C 0 . DP=26;I16=9,15,0,0,806,28552,0,0,1440,86400,0,0,466,10582,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,24,207:8:0 0,12,126:4:0 17 799 . C 0 . DP=26;I16=10,15,0,0,947,36407,0,0,1500,90000,0,0,491,11185,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,234:9:0 0,12,137:4:0 17 800 . T 0 . DP=26;I16=11,15,0,0,993,38475,0,0,1529,90841,0,0,495,11199,0,0;QS=3,0;MQSB=0.927041;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,27,255:9:0 0,12,139:4:0 17 801 . G 0 . DP=25;I16=11,14,0,0,938,35854,0,0,1469,87241,0,0,495,11209,0,0;QS=3,0;MQSB=0.929204;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,240:9:0 0,12,145:4:0 17 802 . G 0 . DP=25;I16=11,14,0,0,892,32802,0,0,1469,87241,0,0,495,11239,0,0;QS=3,0;MQSB=0.929204;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,229:9:0 0,12,137:4:0 17 803 . G 0 . DP=25;I16=11,14,0,0,906,33502,0,0,1469,87241,0,0,495,11289,0,0;QS=3,0;MQSB=0.929204;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,237:9:0 0,12,136:4:0 17 804 . G 0 . DP=25;I16=11,12,0,0,868,33326,0,0,1349,80041,0,0,466,10846,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,223:8:0 0,12,138:4:0 17 805 . C 0 . DP=24;I16=9,14,0,0,810,29684,0,0,1380,82800,0,0,482,11208,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,220:8:0 0,12,128:4:0 17 806 . C 0 . DP=25;I16=9,14,0,0,814,29856,0,0,1380,82800,0,0,483,11293,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,214:8:0 0,12,122:4:0 17 807 . A 0 . DP=25;I16=10,15,0,0,939,35997,0,0,1500,90000,0,0,503,11525,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,242:9:0 0,12,146:4:0 17 808 . G 0 . DP=25;I16=10,15,0,0,918,34720,0,0,1500,90000,0,0,505,11591,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,238:9:0 0,12,136:4:0 17 809 . G 0 . DP=25;I16=10,15,0,0,926,34932,0,0,1500,90000,0,0,506,11626,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,226:9:0 0,12,136:4:0 17 810 . G C, 0 . DP=25;I16=10,13,0,1,824,30436,14,196,1380,82800,60,3600,480,11288,14,196;QS=2.96552,0.0344828,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0 PL:DP:DV 0,21,255,33,255,255:12:1 0,24,216,24,216,216:8:0 0,12,132,12,132,132:4:0 17 811 . A 0 . DP=25;I16=9,14,0,0,808,29404,0,0,1380,82800,0,0,484,11294,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,204:7:0 0,12,139:4:0 17 812 . A 0 . DP=25;I16=10,15,0,0,831,29515,0,0,1500,90000,0,0,500,11392,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,216:9:0 0,12,139:4:0 17 813 . C 0 . DP=25;I16=10,15,0,0,915,34093,0,0,1500,90000,0,0,497,11307,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,239:9:0 0,12,138:4:0 17 814 . T 0 . DP=25;I16=10,15,0,0,987,39343,0,0,1500,90000,0,0,494,11244,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,255:9:0 0,12,148:4:0 17 815 . T 0 . DP=25;I16=10,15,0,0,894,32670,0,0,1500,90000,0,0,491,11203,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,235:9:0 0,12,143:4:0 17 816 . T 0 . DP=25;I16=10,15,0,0,898,32990,0,0,1500,90000,0,0,488,11184,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,239:9:0 0,12,136:4:0 17 817 . C 0 . DP=24;I16=10,14,0,0,898,34256,0,0,1440,86400,0,0,485,11137,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,235:9:0 0,12,124:4:0 17 818 . T 0 . DP=23;I16=9,14,0,0,883,34371,0,0,1380,82800,0,0,484,11110,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,225:8:0 0,12,143:4:0 17 819 . G 0 . DP=23;I16=9,13,0,0,832,31932,0,0,1320,79200,0,0,461,10573,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,24,217:8:0 0,12,142:4:0 17 820 . G 0 . DP=24;I16=10,14,0,0,844,30848,0,0,1440,86400,0,0,484,11114,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,230:9:0 0,12,135:4:0 17 821 . G 0 . DP=24;I16=10,14,0,0,852,31572,0,0,1440,86400,0,0,484,11098,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,234:9:0 0,12,142:4:0 17 822 . G 0 . DP=25;I16=10,15,0,0,879,31785,0,0,1500,90000,0,0,481,10955,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,220:9:0 0,12,127:4:0 17 823 . T 0 . DP=25;I16=10,15,0,0,844,29686,0,0,1500,90000,0,0,478,10786,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,217:9:0 0,12,127:4:0 17 824 . C 0 . DP=25;I16=9,14,0,0,824,30252,0,0,1380,82800,0,0,427,9477,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,215:9:0 0,9,98:3:0 17 825 . A 0 . DP=25;I16=10,15,0,0,881,31665,0,0,1500,90000,0,0,467,10277,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,234:9:0 0,12,116:4:0 17 826 . T 0 . DP=25;I16=10,15,0,0,826,28364,0,0,1500,90000,0,0,461,10039,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,250:12:0 0,27,232:9:0 0,12,117:4:0 17 827 . A 0 . DP=25;I16=10,15,0,0,851,29477,0,0,1500,90000,0,0,455,9829,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,227:9:0 0,12,123:4:0 17 828 . T C, 0 . DP=25;I16=2,4,8,11,199,6917,656,23340,360,21600,1140,68400,116,2716,333,6931;QS=0.597777,2.40222,0;VDB=0.842082;SGB=-4.20907;RPB=0.950652;MQB=1;MQSB=1;BQB=0.929717;MQ0F=0 PL:DP:DV 211,0,35,217,65,255:12:10 116,0,91,128,106,213:9:5 120,12,0,120,12,120:4:4 17 829 . T 0 . DP=24;I16=10,13,0,0,863,32931,0,0,1380,82800,0,0,418,8818,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,242:8:0 0,12,132:4:0 17 830 . C 0 . DP=24;I16=10,14,0,0,910,34966,0,0,1440,86400,0,0,437,9267,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,24,220:8:0 0,12,134:4:0 17 831 . T 0 . DP=24;I16=10,14,0,0,906,34886,0,0,1440,86400,0,0,431,9119,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,24,243:8:0 0,12,133:4:0 17 832 . C 0 . DP=24;I16=10,14,0,0,888,33634,0,0,1440,86400,0,0,425,8999,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,24,220:8:0 0,12,123:4:0 17 833 . T 0 . DP=25;I16=10,14,0,0,820,29920,0,0,1440,86400,0,0,418,8856,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,24,224:8:0 0,12,113:4:0 17 834 . G A, 0 . DP=25;I16=2,3,7,10,164,5898,590,21124,300,18000,1020,61200,104,2296,305,6441;QS=0.520056,2.47994,0;VDB=0.788006;SGB=-4.01214;RPB=0.999233;MQB=1;MQSB=1;BQB=0.821668;MQ0F=0 PL:DP:DV 185,0,46,191,73,246:11:9 128,0,59,137,74,193:8:5 89,9,0,89,9,89:3:3 17 835 . T 0 . DP=26;I16=9,15,0,0,767,26675,0,0,1440,86400,0,0,406,8652,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,240:12:0 0,24,213:8:0 0,12,98:4:0 17 836 . G 0 . DP=23;I16=8,15,0,0,833,30561,0,0,1380,82800,0,0,403,8541,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,243:11:0 0,24,214:8:0 0,12,130:4:0 17 837 . T 0 . DP=23;I16=8,15,0,0,843,31499,0,0,1380,82800,0,0,400,8456,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,241:11:0 0,24,221:8:0 0,12,143:4:0 17 838 . T 0 . DP=23;I16=8,15,0,0,870,33172,0,0,1380,82800,0,0,397,8397,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,252:11:0 0,24,224:8:0 0,12,134:4:0 17 839 . G 0 . DP=24;I16=8,15,0,0,862,32774,0,0,1380,82800,0,0,393,8315,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,249:11:0 0,24,212:8:0 0,12,142:4:0 17 840 . A 0 . DP=25;I16=9,15,0,0,876,32682,0,0,1440,86400,0,0,375,7731,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,243:11:0 0,21,196:7:0 0,18,180:6:0 17 841 . T 0 . DP=25;I16=9,16,0,0,861,30879,0,0,1500,90000,0,0,396,8260,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,213:11:0 0,24,226:8:0 0,18,185:6:0 17 842 . T 0 . DP=24;I16=9,15,0,0,872,31990,0,0,1440,86400,0,0,393,8199,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,227:10:0 0,24,231:8:0 0,18,175:6:0 17 843 . C 0 . DP=24;I16=9,15,0,0,844,30604,0,0,1440,86400,0,0,390,8172,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,228:10:0 0,24,229:8:0 0,18,160:6:0 17 844 . T 0 . DP=24;I16=9,15,0,0,900,34094,0,0,1440,86400,0,0,386,8128,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,227:10:0 0,24,238:8:0 0,18,189:6:0 17 845 . G 0 . DP=25;I16=10,15,0,0,916,33866,0,0,1500,90000,0,0,382,8116,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,239:10:0 0,24,225:8:0 0,21,196:7:0 17 846 . G 0 . DP=24;I16=9,14,0,0,802,28414,0,0,1380,82800,0,0,354,7460,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,223:9:0 0,24,208:8:0 0,18,164:6:0 17 847 . T 0 . DP=24;I16=8,15,0,0,809,29007,0,0,1380,82800,0,0,377,8083,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,210:9:0 0,24,219:8:0 0,18,149:6:0 17 848 . G 0 . DP=23;I16=8,15,0,0,829,30351,0,0,1380,82800,0,0,384,8138,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,199:8:0 0,27,243:9:0 0,18,167:6:0 17 849 . G 0 . DP=22;I16=8,12,0,0,684,23844,0,0,1200,72000,0,0,349,7517,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,160:6:0 0,27,233:9:0 0,15,140:5:0 17 850 . T 0 . DP=21;I16=6,14,0,0,706,25508,0,0,1200,72000,0,0,360,7568,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,153:6:0 0,24,226:8:0 0,18,147:6:0 17 851 . G 0 . DP=21;I16=7,14,0,0,707,24667,0,0,1260,75600,0,0,386,8254,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,140:6:0 0,27,233:9:0 0,18,156:6:0 17 852 . G 0 . DP=21;I16=7,13,0,0,687,24223,0,0,1200,72000,0,0,368,7976,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,147:6:0 0,27,222:9:0 0,15,146:5:0 17 853 . A 0 . DP=21;I16=7,14,0,0,721,25603,0,0,1260,75600,0,0,388,8442,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,146:6:0 0,27,223:9:0 0,18,166:6:0 17 854 . A 0 . DP=21;I16=7,14,0,0,725,25843,0,0,1260,75600,0,0,389,8517,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,179:7:0 0,24,209:8:0 0,18,168:6:0 17 855 . A 0 . DP=21;I16=7,14,0,0,683,23803,0,0,1260,75600,0,0,391,8611,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,171:7:0 0,24,204:8:0 0,18,152:6:0 17 856 . C 0 . DP=21;I16=7,14,0,0,763,28293,0,0,1260,75600,0,0,392,8676,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,192:7:0 0,24,222:8:0 0,18,172:6:0 17 857 . A 0 . DP=22;I16=8,14,0,0,786,28480,0,0,1320,79200,0,0,393,8763,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,182:7:0 0,24,220:8:0 0,21,194:7:0 17 858 . A 0 . DP=22;I16=8,14,0,0,816,31358,0,0,1320,79200,0,0,395,8873,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,192:7:0 0,24,236:8:0 0,21,198:7:0 17 859 . G 0 . DP=22;I16=8,14,0,0,824,31356,0,0,1320,79200,0,0,395,8907,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,202:7:0 0,24,223:8:0 0,21,203:7:0 17 860 . A 0 . DP=22;I16=8,13,0,0,743,26985,0,0,1260,75600,0,0,369,8291,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,184:7:0 0,21,202:7:0 0,21,190:7:0 17 861 . C 0 . DP=22;I16=8,14,0,0,770,28376,0,0,1320,79200,0,0,393,8899,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,206:8:0 0,24,216:8:0 0,18,161:6:0 17 862 . T 0 . DP=22;I16=8,14,0,0,762,27500,0,0,1320,79200,0,0,393,8905,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,188:8:0 0,24,213:8:0 0,18,177:6:0 17 863 . G 0 . DP=23;I16=9,14,0,0,836,30660,0,0,1380,82800,0,0,393,8935,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,228:9:0 0,24,221:8:0 0,18,180:6:0 17 864 . T 0 . DP=22;I16=9,13,0,0,795,29085,0,0,1320,79200,0,0,395,8989,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,215:8:0 0,24,221:8:0 0,18,180:6:0 17 865 . C 0 . DP=22;I16=8,14,0,0,785,28475,0,0,1320,79200,0,0,397,9015,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,227:9:0 0,21,193:7:0 0,18,177:6:0 17 866 . C 0 . DP=21;I16=7,13,0,0,694,24890,0,0,1200,72000,0,0,395,8985,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,220:8:0 0,18,149:6:0 0,18,177:6:0 17 867 . C 0 . DP=21;I16=7,14,0,0,761,28443,0,0,1260,75600,0,0,403,9023,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,223:9:0 0,18,182:6:0 0,18,187:6:0 17 868 . A 0 . DP=21;I16=7,14,0,0,799,31183,0,0,1260,75600,0,0,406,9054,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,228:9:0 0,18,189:6:0 0,18,202:6:0 17 869 . G 0 . DP=21;I16=7,14,0,0,795,30733,0,0,1260,75600,0,0,409,9103,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,236:9:0 0,18,190:6:0 0,18,187:6:0 17 870 . C 0 . DP=21;I16=7,13,0,0,758,29080,0,0,1200,72000,0,0,403,9089,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,226:8:0 0,18,183:6:0 0,18,187:6:0 17 871 . C 0 . DP=21;I16=7,14,0,0,769,29383,0,0,1260,75600,0,0,413,9155,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,232:9:0 0,18,176:6:0 0,18,192:6:0 17 872 . T 0 . DP=21;I16=7,14,0,0,785,29879,0,0,1260,75600,0,0,413,9109,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,228:9:0 0,18,187:6:0 0,18,188:6:0 17 873 . G 0 . DP=21;I16=7,14,0,0,768,28506,0,0,1260,75600,0,0,413,9083,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,233:9:0 0,18,172:6:0 0,18,182:6:0 17 874 . G 0 . DP=21;I16=7,12,0,0,700,26434,0,0,1140,68400,0,0,374,8234,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,227:8:0 0,15,143:5:0 0,18,189:6:0 17 875 . G 0 . DP=21;I16=7,14,0,0,717,25659,0,0,1260,75600,0,0,411,8995,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,227:9:0 0,18,163:6:0 0,18,159:6:0 17 876 . T 0 . DP=21;I16=7,14,0,0,719,25261,0,0,1260,75600,0,0,410,8984,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,222:9:0 0,18,156:6:0 0,18,173:6:0 17 877 . G 0 . DP=21;I16=7,14,0,0,747,27419,0,0,1260,75600,0,0,409,8995,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,235:9:0 0,18,162:6:0 0,18,176:6:0 17 878 . A 0 . DP=21;I16=7,13,0,0,738,27650,0,0,1200,72000,0,0,391,8739,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,216:8:0 0,18,172:6:0 0,18,191:6:0 17 879 . T 0 . DP=21;I16=7,13,0,0,731,26927,0,0,1200,72000,0,0,389,8759,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,214:8:0 0,18,175:6:0 0,18,184:6:0 17 880 . A 0 . DP=21;I16=7,14,0,0,737,26481,0,0,1260,75600,0,0,405,9109,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,227:9:0 0,18,165:6:0 0,18,172:6:0 17 881 . C 0 . DP=20;I16=7,13,0,0,742,27898,0,0,1200,72000,0,0,404,9154,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,231:8:0 0,18,164:6:0 0,18,183:6:0 17 882 . A 0 . DP=20;I16=7,13,0,0,776,30564,0,0,1200,72000,0,0,403,9217,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,225:8:0 0,18,188:6:0 0,18,200:6:0 17 883 . G 0 . DP=20;I16=7,13,0,0,702,25898,0,0,1200,72000,0,0,401,9247,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,214:8:0 0,18,175:6:0 0,18,176:6:0 17 884 . C 0 . DP=19;I16=7,12,0,0,629,21449,0,0,1140,68400,0,0,400,9292,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,201:8:0 0,18,155:6:0 0,15,144:5:0 17 885 . G 0 . DP=18;I16=7,11,0,0,605,20851,0,0,1080,64800,0,0,400,9350,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,215:8:0 0,15,140:5:0 0,15,130:5:0 17 886 . A 0 . DP=18;I16=7,11,0,0,681,26145,0,0,1080,64800,0,0,400,9420,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,222:8:0 0,15,164:5:0 0,15,163:5:0 17 887 . G 0 . DP=18;I16=7,11,0,0,631,22891,0,0,1080,64800,0,0,399,9451,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,222:8:0 0,15,138:5:0 0,15,149:5:0 17 888 . A 0 . DP=18;I16=7,10,0,0,593,21563,0,0,1020,61200,0,0,373,8867,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,216:8:0 0,15,132:5:0 0,12,127:4:0 17 889 . C 0 . DP=18;I16=7,11,0,0,624,22732,0,0,1080,64800,0,0,396,9492,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,213:8:0 0,15,134:5:0 0,15,160:5:0 17 890 . C 0 . DP=19;I16=7,11,0,0,616,22426,0,0,1080,64800,0,0,368,8826,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,215:8:0 0,15,138:5:0 0,15,152:5:0 17 891 . C 0 . DP=19;I16=7,11,0,0,661,24891,0,0,1080,64800,0,0,365,8745,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,227:8:0 0,15,141:5:0 0,15,165:5:0 17 892 . C 0 . DP=19;I16=7,12,0,0,691,25761,0,0,1140,68400,0,0,387,9299,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,230:9:0 0,15,146:5:0 0,15,167:5:0 17 893 . A 0 . DP=19;I16=7,12,0,0,673,24521,0,0,1140,68400,0,0,384,9238,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,221:9:0 0,15,142:5:0 0,15,166:5:0 17 894 . T 0 . DP=19;I16=7,12,0,0,670,24268,0,0,1140,68400,0,0,380,9138,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,225:9:0 0,15,134:5:0 0,15,165:5:0 17 895 . C 0 . DP=20;I16=8,12,0,0,712,26186,0,0,1200,72000,0,0,376,9050,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,248:10:0 0,15,149:5:0 0,15,162:5:0 17 896 . T 0 . DP=19;I16=8,10,0,0,684,26696,0,0,1080,64800,0,0,348,8300,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,238:9:0 0,12,141:4:0 0,15,180:5:0 17 897 . C 0 . DP=18;I16=8,10,0,0,693,27001,0,0,1080,64800,0,0,370,8764,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,12,131:4:0 0,15,166:5:0 17 898 . T 0 . DP=18;I16=8,10,0,0,674,25656,0,0,1080,64800,0,0,367,8617,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,243:9:0 0,12,125:4:0 0,15,172:5:0 17 899 . A 0 . DP=18;I16=9,8,0,0,597,21451,0,0,1020,61200,0,0,340,7858,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,239:9:0 0,9,99:3:0 0,15,145:5:0 17 900 . C 0 . DP=18;I16=9,9,0,0,658,24550,0,0,1080,64800,0,0,364,8362,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,98:3:0 0,15,166:5:0 17 901 . C 0 . DP=18;I16=9,9,0,0,680,26004,0,0,1080,64800,0,0,363,8255,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,99:3:0 0,15,169:5:0 17 902 . A 0 . DP=18;I16=9,9,0,0,681,26253,0,0,1080,64800,0,0,362,8162,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,253:10:0 0,9,115:3:0 0,15,174:5:0 17 903 . A 0 . DP=18;I16=9,8,0,0,645,24625,0,0,1020,61200,0,0,336,7458,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,250:9:0 0,9,101:3:0 0,15,171:5:0 17 904 . A 0 . DP=18;I16=9,9,0,0,640,23412,0,0,1080,64800,0,0,359,7969,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,249:10:0 0,9,85:3:0 0,15,174:5:0 17 905 . A 0 . DP=18;I16=9,9,0,0,675,25673,0,0,1080,64800,0,0,357,7871,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,103:3:0 0,15,172:5:0 17 906 . A 0 . DP=18;I16=9,9,0,0,669,25193,0,0,1080,64800,0,0,355,7789,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,102:3:0 0,15,166:5:0 17 907 . A 0 . DP=18;I16=9,9,0,0,661,24541,0,0,1080,64800,0,0,353,7723,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,94:3:0 0,15,165:5:0 17 908 . T 0 . DP=18;I16=9,9,0,0,673,25579,0,0,1080,64800,0,0,351,7673,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,83:3:0 0,15,168:5:0 17 909 . T 0 . DP=18;I16=9,9,0,0,653,23847,0,0,1080,64800,0,0,348,7590,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,95:3:0 0,15,161:5:0 17 910 . A 0 . DP=18;I16=9,9,0,0,652,23790,0,0,1080,64800,0,0,344,7476,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,248:10:0 0,9,104:3:0 0,15,162:5:0 17 911 . A 0 . DP=18;I16=9,9,0,0,688,26440,0,0,1080,64800,0,0,340,7382,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,109:3:0 0,15,168:5:0 17 912 . A 0 . DP=18;I16=9,9,0,0,691,26705,0,0,1080,64800,0,0,336,7308,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,115:3:0 0,15,173:5:0 17 913 . A 0 . DP=19;I16=9,10,0,0,692,25762,0,0,1140,68400,0,0,332,7254,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,109:3:0 0,15,171:5:0 17 914 . A 0 . DP=19;I16=9,10,0,0,712,26966,0,0,1140,68400,0,0,329,7221,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,109:3:0 0,15,164:5:0 17 915 . T 0 . DP=18;I16=9,9,0,0,638,23228,0,0,1080,64800,0,0,326,7160,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,247:10:0 0,9,99:3:0 0,15,163:5:0 17 916 . T 0 . DP=19;I16=9,8,0,0,626,23136,0,0,1020,61200,0,0,296,6396,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,241:9:0 0,9,103:3:0 0,15,164:5:0 17 917 . A 0 . DP=19;I16=9,10,0,0,726,27962,0,0,1117,66169,0,0,318,6908,0,0;QS=3,0;MQSB=0.934728;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,125:4:0 0,15,171:5:0 17 918 . G 0 . DP=19;I16=9,10,0,0,688,25456,0,0,1117,66169,0,0,314,6818,0,0;QS=3,0;MQSB=0.934728;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,129:4:0 0,15,147:5:0 17 919 . C 0 . DP=18;I16=8,10,0,0,672,25786,0,0,1057,62569,0,0,311,6751,0,0;QS=3,0;MQSB=0.938795;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,92:3:0 0,15,152:5:0 17 920 . T 0 . DP=18;I16=8,10,0,0,681,26113,0,0,1057,62569,0,0,308,6706,0,0;QS=3,0;MQSB=0.938795;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,95:3:0 0,15,171:5:0 17 921 . G 0 . DP=17;I16=7,10,0,0,617,22841,0,0,997,58969,0,0,304,6582,0,0;QS=3,0;MQSB=0.943335;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,6,47:2:0 0,15,164:5:0 17 922 . G 0 . DP=16;I16=7,8,0,0,560,21156,0,0,877,51769,0,0,276,5852,0,0;QS=3,0;MQSB=0.921243;MQ0F=0 PL:DP:DV 0,24,243:8:0 0,6,64:2:0 0,15,151:5:0 17 923 . G 0 . DP=16;I16=7,8,0,0,562,21350,0,0,877,51769,0,0,273,5765,0,0;QS=3,0;MQSB=0.921243;MQ0F=0 PL:DP:DV 0,24,245:8:0 0,6,65:2:0 0,15,144:5:0 17 924 . C 0 . DP=16;I16=7,9,0,0,603,22955,0,0,937,55369,0,0,295,6321,0,0;QS=3,0;MQSB=0.933674;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,6,57:2:0 0,15,157:5:0 17 925 . A 0 . DP=16;I16=7,9,0,0,576,21618,0,0,937,55369,0,0,291,6219,0,0;QS=3,0;MQSB=0.933674;MQ0F=0 PL:DP:DV 0,27,244:9:0 0,6,58:2:0 0,15,163:5:0 17 926 . T 0 . DP=16;I16=7,9,0,0,585,22015,0,0,937,55369,0,0,287,6133,0,0;QS=3,0;MQSB=0.933674;MQ0F=0 PL:DP:DV 0,27,241:9:0 0,6,63:2:0 0,15,161:5:0 17 927 . G 0 . DP=17;I16=7,10,0,0,641,24481,0,0,997,58969,0,0,283,6063,0,0;QS=3,0;MQSB=0.943335;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,9,82:3:0 0,15,158:5:0 17 928 . G 0 . DP=17;I16=7,10,0,0,625,23195,0,0,997,58969,0,0,280,6010,0,0;QS=3,0;MQSB=0.943335;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,9,84:3:0 0,15,150:5:0 17 929 . T 0 . DP=16;I16=7,9,0,0,580,21580,0,0,937,55369,0,0,277,5925,0,0;QS=3,0;MQSB=0.933674;MQ0F=0 PL:DP:DV 0,27,248:9:0 0,9,79:3:0 0,12,128:4:0 17 930 . G 0 . DP=16;I16=7,8,0,0,565,21561,0,0,877,51769,0,0,249,5233,0,0;QS=3,0;MQSB=0.921243;MQ0F=0 PL:DP:DV 0,24,247:8:0 0,9,82:3:0 0,12,127:4:0 17 931 . G 0 . DP=16;I16=7,9,0,0,553,19935,0,0,937,55369,0,0,271,5809,0,0;QS=3,0;MQSB=0.933674;MQ0F=0 PL:DP:DV 0,27,244:9:0 0,9,80:3:0 0,12,113:4:0 17 932 . T 0 . DP=16;I16=7,9,0,0,558,20128,0,0,937,55369,0,0,268,5778,0,0;QS=3,0;MQSB=0.933674;MQ0F=0 PL:DP:DV 0,27,229:9:0 0,9,89:3:0 0,12,122:4:0 17 933 . G 0 . DP=16;I16=7,8,0,0,558,20926,0,0,877,51769,0,0,239,5091,0,0;QS=3,0;MQSB=0.921243;MQ0F=0 PL:DP:DV 0,24,241:8:0 0,9,94:3:0 0,12,116:4:0 17 934 . C 0 . DP=15;I16=7,8,0,0,548,20256,0,0,877,51769,0,0,261,5673,0,0;QS=3,0;MQSB=0.921243;MQ0F=0 PL:DP:DV 0,27,244:9:0 0,9,83:3:0 0,9,99:3:0 17 935 . A 0 . DP=15;I16=7,8,0,0,534,19780,0,0,846,49010,0,0,259,5647,0,0;QS=3,0;MQSB=0.720273;MQ0F=0 PL:DP:DV 0,27,233:9:0 0,9,86:3:0 0,9,101:3:0 17 936 . T 0 . DP=15;I16=7,8,0,0,579,22499,0,0,846,49010,0,0,257,5589,0,0;QS=3,0;MQSB=0.720273;MQ0F=0 PL:DP:DV 0,27,252:9:0 0,9,91:3:0 0,9,100:3:0 17 937 . G 0 . DP=16;I16=8,7,0,0,550,20650,0,0,846,49010,0,0,232,5022,0,0;QS=3,0;MQSB=0.651439;MQ0F=0 PL:DP:DV 0,24,228:8:0 0,9,84:3:0 0,12,115:4:0 17 938 . C 0 . DP=16;I16=8,7,0,0,553,20669,0,0,846,49010,0,0,231,5001,0,0;QS=3,0;MQSB=0.651439;MQ0F=0 PL:DP:DV 0,24,234:8:0 0,9,77:3:0 0,12,119:4:0 17 939 . C 0 . DP=16;I16=8,8,0,0,582,22100,0,0,906,52610,0,0,250,5392,0,0;QS=3,0;MQSB=0.702619;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,9,71:3:0 0,12,115:4:0 17 940 . T 0 . DP=15;I16=8,7,0,0,583,23339,0,0,846,49010,0,0,248,5320,0,0;QS=3,0;MQSB=0.651439;MQ0F=0 PL:DP:DV 0,27,252:9:0 0,6,71:2:0 0,12,124:4:0 17 941 . G 0 . DP=14;I16=7,7,0,0,501,18707,0,0,786,45410,0,0,246,5216,0,0;QS=3,0;MQSB=0.714506;MQ0F=0 PL:DP:DV 0,27,240:9:0 0,6,59:2:0 0,9,93:3:0 17 942 . T 0 . DP=14;I16=7,7,0,0,525,19799,0,0,786,45410,0,0,244,5128,0,0;QS=3,0;MQSB=0.714506;MQ0F=0 PL:DP:DV 0,27,237:9:0 0,6,70:2:0 0,9,94:3:0 17 943 . A 0 . DP=14;I16=7,7,0,0,545,21399,0,0,786,45410,0,0,242,5056,0,0;QS=3,0;MQSB=0.714506;MQ0F=0 PL:DP:DV 0,27,248:9:0 0,6,71:2:0 0,9,93:3:0 17 944 . G 0 . DP=14;I16=7,7,0,0,529,20143,0,0,786,45410,0,0,240,5000,0,0;QS=3,0;MQSB=0.714506;MQ0F=0 PL:DP:DV 0,27,249:9:0 0,6,65:2:0 0,9,95:3:0 17 945 . T 0 . DP=14;I16=7,7,0,0,523,19621,0,0,786,45410,0,0,238,4960,0,0;QS=3,0;MQSB=0.714506;MQ0F=0 PL:DP:DV 0,27,239:9:0 0,6,70:2:0 0,9,95:3:0 17 946 . C 0 . DP=13;I16=6,6,0,0,455,17581,0,0,666,38210,0,0,223,4739,0,0;QS=3,0;MQSB=0.660716;MQ0F=0 PL:DP:DV 0,24,240:8:0 0,6,56:2:0 0,6,70:2:0 17 947 . C 0 . DP=14;I16=7,7,0,0,509,19007,0,0,755,42651,0,0,238,4928,0,0;QS=3,0;MQSB=0.925999;MQ0F=0 PL:DP:DV 0,27,251:9:0 0,9,83:3:0 0,6,66:2:0 17 948 . C 0 . DP=14;I16=7,7,0,0,520,20050,0,0,755,42651,0,0,237,4887,0,0;QS=3,0;MQSB=0.925999;MQ0F=0 PL:DP:DV 0,27,254:9:0 0,9,78:3:0 0,6,69:2:0 17 949 . A 0 . DP=15;I16=7,8,0,0,547,20705,0,0,815,46251,0,0,236,4864,0,0;QS=3,0;MQSB=0.959011;MQ0F=0 PL:DP:DV 0,30,250:10:0 0,9,90:3:0 0,6,72:2:0 17 950 . G 0 . DP=15;I16=6,8,0,0,563,22871,0,0,786,45410,0,0,231,4835,0,0;QS=3,0;MQSB=0.740818;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,6,71:2:0 0,6,72:2:0 17 951 . C 0 . DP=16;I16=7,8,0,0,554,21080,0,0,846,49010,0,0,230,4840,0,0;QS=3,0;MQSB=0.720273;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,6,65:2:0 0,6,73:2:0 17 952 . T 0 . DP=16;I16=8,8,0,0,595,22565,0,0,875,49851,0,0,237,4913,0,0;QS=3,0;MQSB=0.934676;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,93:3:0 0,6,73:2:0 17 953 . A 0 . DP=16;I16=8,8,0,0,567,20515,0,0,875,49851,0,0,237,4921,0,0;QS=3,0;MQSB=0.934676;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,91:3:0 0,6,69:2:0 17 954 . T 0 . DP=15;I16=7,8,0,0,544,20412,0,0,815,46251,0,0,238,4948,0,0;QS=3,0;MQSB=0.959011;MQ0F=0 PL:DP:DV 0,30,249:10:0 0,9,94:3:0 0,6,69:2:0 17 955 . T 0 . DP=15;I16=7,8,0,0,543,19953,0,0,815,46251,0,0,239,4993,0,0;QS=3,0;MQSB=0.959011;MQ0F=0 PL:DP:DV 0,30,246:10:0 0,9,95:3:0 0,6,68:2:0 17 956 . C 0 . DP=15;I16=6,8,0,0,543,21255,0,0,786,45410,0,0,229,4935,0,0;QS=3,0;MQSB=0.740818;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,6,62:2:0 0,6,70:2:0 17 957 . A 0 . DP=15;I16=7,8,0,0,504,17684,0,0,815,46251,0,0,241,5137,0,0;QS=3,0;MQSB=0.959011;MQ0F=0 PL:DP:DV 0,30,240:10:0 0,9,74:3:0 0,6,67:2:0 17 958 . C 0 . DP=14;I16=6,8,0,0,505,18981,0,0,755,42651,0,0,243,5235,0,0;QS=3,0;MQSB=0.981425;MQ0F=0 PL:DP:DV 0,30,253:10:0 0,9,78:3:0 0,3,39:1:0 17 959 . A 0 . DP=14;I16=5,8,0,0,519,20885,0,0,726,41810,0,0,231,5153,0,0;QS=3,0;MQSB=0.765017;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,6,72:2:0 0,3,38:1:0 17 960 . G 0 . DP=15;I16=6,9,0,0,539,19901,0,0,815,46251,0,0,247,5479,0,0;QS=3,0;MQSB=0.99308;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,106:4:0 0,3,41:1:0 17 961 . T 0 . DP=14;I16=6,8,0,0,523,19835,0,0,755,42651,0,0,250,5574,0,0;QS=3,0;MQSB=0.981425;MQ0F=0 PL:DP:DV 0,27,248:9:0 0,12,114:4:0 0,3,39:1:0 17 962 . G 0 . DP=14;I16=5,8,0,0,498,19194,0,0,726,41810,0,0,236,5394,0,0;QS=3,0;MQSB=0.765017;MQ0F=0 PL:DP:DV 0,27,253:9:0 0,9,91:3:0 0,3,36:1:0 17 963 . C 0 . DP=13;I16=5,8,0,0,500,19610,0,0,695,39051,0,0,256,5754,0,0;QS=3,0;MQSB=0.997325;MQ0F=0 PL:DP:DV 0,24,234:8:0 0,12,118:4:0 0,3,40:1:0 17 964 . T 0 . DP=13;I16=5,8,0,0,512,20430,0,0,695,39051,0,0,259,5835,0,0;QS=3,0;MQSB=0.997325;MQ0F=0 PL:DP:DV 0,24,227:8:0 0,12,128:4:0 0,3,42:1:0 17 965 . G 0 . DP=13;I16=4,8,0,0,467,18429,0,0,666,38210,0,0,241,5477,0,0;QS=3,0;MQSB=0.793923;MQ0F=0 PL:DP:DV 0,24,228:8:0 0,9,93:3:0 0,3,40:1:0 17 966 . A 0 . DP=13;I16=4,8,0,0,443,16785,0,0,666,38210,0,0,242,5490,0,0;QS=3,0;MQSB=0.793923;MQ0F=0 PL:DP:DV 0,24,212:8:0 0,9,95:3:0 0,3,40:1:0 17 967 . G 0 . DP=13;I16=4,8,0,0,464,18036,0,0,666,38210,0,0,243,5513,0,0;QS=3,0;MQSB=0.793923;MQ0F=0 PL:DP:DV 0,24,230:8:0 0,9,91:3:0 0,3,41:1:0 17 968 . G 0 . DP=13;I16=4,8,0,0,441,16549,0,0,666,38210,0,0,244,5546,0,0;QS=3,0;MQSB=0.793923;MQ0F=0 PL:DP:DV 0,24,218:8:0 0,9,87:3:0 0,3,42:1:0 17 969 . T 0 . DP=13;I16=4,8,0,0,450,16970,0,0,666,38210,0,0,245,5589,0,0;QS=3,0;MQSB=0.793923;MQ0F=0 PL:DP:DV 0,24,220:8:0 0,9,98:3:0 0,3,31:1:0 17 970 . G 0 . DP=13;I16=4,7,0,0,413,15579,0,0,629,36841,0,0,220,4968,0,0;QS=3,0;MQSB=0.924449;MQ0F=0 PL:DP:DV 0,24,221:8:0 0,6,71:2:0 0,3,33:1:0 17 971 . G 0 . DP=13;I16=4,8,0,0,459,17711,0,0,666,38210,0,0,245,5609,0,0;QS=3,0;MQSB=0.793923;MQ0F=0 PL:DP:DV 0,24,223:8:0 0,9,93:3:0 0,3,38:1:0 17 972 . G 0 . DP=13;I16=4,8,0,0,446,17192,0,0,666,38210,0,0,245,5637,0,0;QS=3,0;MQSB=0.793923;MQ0F=0 PL:DP:DV 0,24,210:8:0 0,9,96:3:0 0,3,39:1:0 17 973 . A 0 . DP=12;I16=4,7,0,0,402,15018,0,0,606,34610,0,0,246,5676,0,0;QS=3,0;MQSB=0.763675;MQ0F=0 PL:DP:DV 0,21,192:7:0 0,9,89:3:0 0,3,42:1:0 17 974 . A 0 . DP=12;I16=4,7,0,0,417,16273,0,0,606,34610,0,0,247,5725,0,0;QS=3,0;MQSB=0.763675;MQ0F=0 PL:DP:DV 0,21,198:7:0 0,9,96:3:0 0,3,42:1:0 17 975 . G 0 . DP=13;I16=4,8,0,0,454,17560,0,0,666,38210,0,0,247,5733,0,0;QS=3,0;MQSB=0.793923;MQ0F=0 PL:DP:DV 0,24,213:8:0 0,9,95:3:0 0,3,42:1:0 17 976 . A 0 . DP=13;I16=4,8,0,0,431,16083,0,0,666,38210,0,0,248,5750,0,0;QS=3,0;MQSB=0.793923;MQ0F=0 PL:DP:DV 0,24,227:8:0 0,9,70:3:0 0,3,41:1:0 17 977 . T 0 . DP=13;I16=4,8,0,0,438,16316,0,0,666,38210,0,0,248,5726,0,0;QS=3,0;MQSB=0.793923;MQ0F=0 PL:DP:DV 0,24,210:8:0 0,9,93:3:0 0,3,37:1:0 17 978 . G 0 . DP=12;I16=4,8,0,0,430,16060,0,0,666,38210,0,0,248,5710,0,0;QS=3,0;MQSB=0.793923;MQ0F=0 PL:DP:DV 0,24,224:8:0 0,9,75:3:0 0,3,40:1:0 17 979 . C 0 . DP=13;I16=4,8,0,0,453,17461,0,0,689,40441,0,0,223,5077,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,27,232:9:0 0,6,72:2:0 0,3,41:1:0 17 980 . T 0 . DP=13;I16=4,8,0,0,438,16412,0,0,689,40441,0,0,224,5078,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,27,220:9:0 0,6,76:2:0 0,3,43:1:0 17 981 . T 0 . DP=13;I16=4,9,0,0,484,18602,0,0,726,41810,0,0,250,5714,0,0;QS=3,0;MQSB=0.826565;MQ0F=0 PL:DP:DV 0,27,224:9:0 0,9,97:3:0 0,3,41:1:0 17 982 . G 0 . DP=14;I16=4,10,0,0,520,20128,0,0,786,45410,0,0,250,5686,0,0;QS=3,0;MQSB=0.852144;MQ0F=0 PL:DP:DV 0,30,237:10:0 0,9,99:3:0 0,3,40:1:0 17 983 . A 0 . DP=14;I16=4,10,0,0,570,23360,0,0,786,45410,0,0,251,5671,0,0;QS=3,0;MQSB=0.852144;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,101:3:0 0,3,42:1:0 17 984 . G 0 . DP=15;I16=4,10,0,0,529,20459,0,0,786,45410,0,0,252,5670,0,0;QS=3,0;MQSB=0.852144;MQ0F=0 PL:DP:DV 0,30,253:10:0 0,9,88:3:0 0,3,43:1:0 17 985 . C 0 . DP=17;I16=4,13,0,0,580,20280,0,0,966,56210,0,0,261,5747,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,33,246:11:0 0,12,100:4:0 0,6,65:2:0 17 986 . C A, 0 . DP=17;I16=3,12,0,1,519,18353,13,169,846,49010,60,3600,235,5101,4,16;QS=2.95873,0.0412698,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.921781;BQB=1;MQ0F=0 PL:DP:DV 0,16,197,27,200,201:10:1 0,12,108,12,108,108:4:0 0,6,69,6,69,69:2:0 17 987 . C 0 . DP=17;I16=4,13,0,0,634,23914,0,0,966,56210,0,0,267,5755,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,107:4:0 0,6,69:2:0 17 988 . A 0 . DP=17;I16=4,13,0,0,633,24341,0,0,966,56210,0,0,269,5737,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,111:4:0 0,6,82:2:0 17 989 . G 0 . DP=17;I16=4,13,0,0,635,24149,0,0,966,56210,0,0,271,5739,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,114:4:0 0,6,80:2:0 17 990 . G 0 . DP=16;I16=4,12,0,0,591,22177,0,0,906,52610,0,0,274,5760,0,0;QS=3,0;MQSB=0.88901;MQ0F=0 PL:DP:DV 0,30,246:10:0 0,12,105:4:0 0,6,83:2:0 17 991 . A 0 . DP=16;I16=3,11,0,0,548,21542,0,0,809,47641,0,0,227,4549,0,0;QS=3,0;MQSB=0.973096;MQ0F=0 PL:DP:DV 0,27,232:9:0 0,9,100:3:0 0,6,80:2:0 17 992 . G 0 . DP=16;I16=4,12,0,0,562,20436,0,0,906,52610,0,0,278,5760,0,0;QS=3,0;MQSB=0.88901;MQ0F=0 PL:DP:DV 0,30,240:10:0 0,12,102:4:0 0,6,76:2:0 17 993 . T 0 . DP=16;I16=4,12,0,0,564,20752,0,0,906,52610,0,0,280,5790,0,0;QS=3,0;MQSB=0.88901;MQ0F=0 PL:DP:DV 0,30,222:10:0 0,12,121:4:0 0,6,73:2:0 17 994 . T 0 . DP=17;I16=5,11,0,0,597,22625,0,0,906,52610,0,0,270,5696,0,0;QS=3,0;MQSB=0.851779;MQ0F=0 PL:DP:DV 0,27,227:9:0 0,12,123:4:0 0,9,103:3:0 17 995 . C 0 . DP=16;I16=4,11,0,0,588,23228,0,0,846,49010,0,0,273,5741,0,0;QS=3,0;MQSB=0.872525;MQ0F=0 PL:DP:DV 0,24,216:8:0 0,12,119:4:0 0,9,110:3:0 17 996 . A 0 . DP=16;I16=4,12,0,0,562,20416,0,0,906,52610,0,0,290,6000,0,0;QS=3,0;MQSB=0.88901;MQ0F=0 PL:DP:DV 0,27,207:9:0 0,12,107:4:0 0,9,113:3:0 17 997 . A 0 . DP=16;I16=4,12,0,0,600,23520,0,0,906,52610,0,0,294,6110,0,0;QS=3,0;MQSB=0.88901;MQ0F=0 PL:DP:DV 0,27,209:9:0 0,12,123:4:0 0,9,121:3:0 17 998 . G 0 . DP=17;I16=4,13,0,0,603,22299,0,0,966,56210,0,0,298,6240,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,30,226:10:0 0,12,99:4:0 0,9,115:3:0 17 999 . G 0 . DP=17;I16=4,12,0,0,575,21519,0,0,906,52610,0,0,302,6390,0,0;QS=3,0;MQSB=0.88901;MQ0F=0 PL:DP:DV 0,27,202:9:0 0,12,118:4:0 0,9,111:3:0 17 1000 . C 0 . DP=17;I16=4,13,0,0,637,24465,0,0,966,56210,0,0,308,6564,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,30,235:10:0 0,12,118:4:0 0,9,109:3:0 17 1001 . T 0 . DP=17;I16=4,13,0,0,647,24907,0,0,966,56210,0,0,312,6708,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,30,228:10:0 0,12,120:4:0 0,9,122:3:0 17 1002 . G 0 . DP=17;I16=4,13,0,0,627,23691,0,0,966,56210,0,0,316,6872,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,30,220:10:0 0,12,123:4:0 0,9,109:3:0 17 1003 . C T, 0 . DP=18;I16=4,13,0,1,633,23953,22,484,966,56210,60,3600,297,6515,21,441;QS=2.9375,0.0625,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.913725;BQB=1;MQ0F=0 PL:DP:DV 0,8,207,27,211,220:10:1 0,15,132,15,132,132:5:0 0,9,102,9,102,102:3:0 17 1004 . A 0 . DP=19;I16=5,14,0,0,653,23107,0,0,1086,63410,0,0,321,7061,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,33,231:11:0 0,15,131:5:0 0,9,98:3:0 17 1005 . A 0 . DP=19;I16=5,14,0,0,670,24380,0,0,1086,63410,0,0,324,7138,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,33,231:11:0 0,15,132:5:0 0,9,113:3:0 17 1006 . T 0 . DP=19;I16=4,14,0,0,659,24869,0,0,1026,59810,0,0,327,7237,0,0;QS=3,0;MQSB=0.913725;MQ0F=0 PL:DP:DV 0,30,220:10:0 0,15,134:5:0 0,9,115:3:0 17 1007 . G 0 . DP=18;I16=4,13,0,0,651,25373,0,0,966,56210,0,0,306,6732,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,27,225:9:0 0,15,138:5:0 0,9,113:3:0 17 1008 . A 0 . DP=18;I16=4,13,0,0,667,27041,0,0,966,56210,0,0,309,6821,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,27,222:9:0 0,15,147:5:0 0,9,118:3:0 17 1009 . G 0 . DP=18;I16=4,13,0,0,635,24431,0,0,966,56210,0,0,312,6928,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,27,213:9:0 0,15,139:5:0 0,9,116:3:0 17 1010 . C 0 . DP=18;I16=4,14,0,0,661,25289,0,0,1026,59810,0,0,339,7629,0,0;QS=3,0;MQSB=0.913725;MQ0F=0 PL:DP:DV 0,30,234:10:0 0,15,126:5:0 0,9,114:3:0 17 1011 . T 0 . DP=18;I16=4,14,0,0,671,25325,0,0,1026,59810,0,0,339,7623,0,0;QS=3,0;MQSB=0.913725;MQ0F=0 PL:DP:DV 0,30,226:10:0 0,15,131:5:0 0,9,118:3:0 17 1012 . A 0 . DP=19;I16=5,14,0,0,639,22891,0,0,1063,61179,0,0,339,7633,0,0;QS=3,0;MQSB=0.990403;MQ0F=0 PL:DP:DV 0,30,201:10:0 0,18,154:6:0 0,9,113:3:0 17 1013 . T 0 . DP=18;I16=5,12,0,0,623,23913,0,0,943,53979,0,0,325,7385,0,0;QS=3,0;MQSB=0.998612;MQ0F=0 PL:DP:DV 0,24,202:8:0 0,18,161:6:0 0,9,115:3:0 17 1014 . G 0 . DP=18;I16=5,13,0,0,656,24596,0,0,1003,57579,0,0,341,7605,0,0;QS=3,0;MQSB=0.995153;MQ0F=0 PL:DP:DV 0,27,218:9:0 0,18,159:6:0 0,9,113:3:0 17 1015 . A 0 . DP=18;I16=5,13,0,0,646,23878,0,0,1003,57579,0,0,342,7618,0,0;QS=3,0;MQSB=0.995153;MQ0F=0 PL:DP:DV 0,27,209:9:0 0,18,164:6:0 0,9,109:3:0 17 1016 . T 0 . DP=17;I16=4,12,0,0,588,22114,0,0,929,54841,0,0,340,7632,0,0;QS=3,0;MQSB=0.971017;MQ0F=0 PL:DP:DV 0,27,212:9:0 0,12,124:4:0 0,9,101:3:0 17 1017 . T 0 . DP=18;I16=5,13,0,0,635,23433,0,0,1026,59810,0,0,346,7694,0,0;QS=3,0;MQSB=0.942222;MQ0F=0 PL:DP:DV 0,27,213:9:0 0,18,155:6:0 0,9,105:3:0 17 1018 . G 0 . DP=18;I16=5,13,0,0,650,24550,0,0,1026,59810,0,0,349,7757,0,0;QS=3,0;MQSB=0.942222;MQ0F=0 PL:DP:DV 0,27,215:9:0 0,18,179:6:0 0,9,91:3:0 17 1019 . C 0 . DP=18;I16=5,12,0,0,555,18561,0,0,966,56210,0,0,327,7213,0,0;QS=3,0;MQSB=0.951229;MQ0F=0 PL:DP:DV 0,24,181:8:0 0,18,143:6:0 0,9,104:3:0 17 1020 . G 0 . DP=19;I16=6,12,0,0,625,22287,0,0,1026,59810,0,0,332,7402,0,0;QS=3,0;MQSB=0.97296;MQ0F=0 PL:DP:DV 0,27,228:9:0 0,18,157:6:0 0,9,87:3:0 17 1021 . C 0 . DP=19;I16=5,13,0,0,625,22761,0,0,1026,59810,0,0,332,7326,0,0;QS=3,0;MQSB=0.942222;MQ0F=0 PL:DP:DV 0,27,220:9:0 0,18,153:6:0 0,9,92:3:0 17 1022 . C 0 . DP=19;I16=6,13,0,0,727,27975,0,0,1086,63410,0,0,360,8034,0,0;QS=3,0;MQSB=0.965977;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,168:6:0 0,9,110:3:0 17 1023 . A 0 . DP=19;I16=6,12,0,0,647,23997,0,0,1026,59810,0,0,338,7510,0,0;QS=3,0;MQSB=0.97296;MQ0F=0 PL:DP:DV 0,27,216:9:0 0,18,168:6:0 0,9,112:3:0 17 1024 . C 0 . DP=20;I16=7,13,0,0,729,27471,0,0,1146,67010,0,0,364,8154,0,0;QS=3,0;MQSB=0.980568;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,169:6:0 0,9,106:3:0 17 1025 . T 0 . DP=20;I16=7,13,0,0,757,29387,0,0,1146,67010,0,0,366,8192,0,0;QS=3,0;MQSB=0.980568;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,181:6:0 0,9,117:3:0 17 1026 . G 0 . DP=20;I16=7,13,0,0,747,28435,0,0,1146,67010,0,0,367,8201,0,0;QS=3,0;MQSB=0.980568;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,169:6:0 0,9,113:3:0 17 1027 . C 0 . DP=20;I16=7,13,0,0,720,26688,0,0,1146,67010,0,0,368,8232,0,0;QS=3,0;MQSB=0.980568;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,145:6:0 0,9,110:3:0 17 1028 . A 0 . DP=19;I16=6,12,0,0,645,23681,0,0,1026,59810,0,0,348,7800,0,0;QS=3,0;MQSB=0.97296;MQ0F=0 PL:DP:DV 0,30,238:10:0 0,15,146:5:0 0,9,99:3:0 17 1029 . C 0 . DP=19;I16=7,12,0,0,699,26817,0,0,1086,63410,0,0,371,8305,0,0;QS=3,0;MQSB=0.985816;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,138:5:0 0,9,111:3:0 17 1030 . T 0 . DP=19;I16=7,12,0,0,749,29789,0,0,1086,63410,0,0,371,8293,0,0;QS=3,0;MQSB=0.985816;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,158:5:0 0,9,122:3:0 17 1031 . T 0 . DP=21;I16=9,12,0,0,748,27726,0,0,1206,70610,0,0,371,8297,0,0;QS=3,0;MQSB=0.997478;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,144:5:0 0,9,113:3:0 17 1032 . T 0 . DP=21;I16=9,12,0,0,761,28033,0,0,1206,70610,0,0,373,8319,0,0;QS=3,0;MQSB=0.997478;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,156:5:0 0,9,111:3:0 17 1033 . G 0 . DP=22;I16=10,12,0,0,773,28227,0,0,1266,74210,0,0,375,8361,0,0;QS=3,0;MQSB=0.999457;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,169:6:0 0,9,101:3:0 17 1034 . G 0 . DP=22;I16=8,12,0,0,703,25617,0,0,1169,69241,0,0,340,7684,0,0;QS=3,0;MQSB=0.953497;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,149:5:0 0,9,102:3:0 17 1035 . C 0 . DP=21;I16=10,11,0,0,719,26103,0,0,1237,73369,0,0,382,8508,0,0;QS=3,0;MQSB=0.927743;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,163:6:0 0,9,94:3:0 17 1036 . C 0 . DP=22;I16=11,10,0,0,756,28390,0,0,1237,73369,0,0,360,7938,0,0;QS=3,0;MQSB=0.939898;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,175:6:0 0,12,135:4:0 17 1037 . T 0 . DP=22;I16=11,11,0,0,839,32737,0,0,1297,76969,0,0,389,8641,0,0;QS=3,0;MQSB=0.936864;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,188:6:0 0,12,138:4:0 17 1038 . G 0 . DP=21;I16=10,10,0,0,752,28750,0,0,1177,69769,0,0,368,8066,0,0;QS=3,0;MQSB=0.931063;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,184:6:0 0,9,107:3:0 17 1039 . G 0 . DP=21;I16=10,11,0,0,775,29373,0,0,1237,73369,0,0,397,8761,0,0;QS=3,0;MQSB=0.927743;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,189:6:0 0,9,111:3:0 17 1040 . A 0 . DP=21;I16=10,11,0,0,759,28007,0,0,1237,73369,0,0,401,8851,0,0;QS=3,0;MQSB=0.927743;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,177:6:0 0,9,107:3:0 17 1041 . C 0 . DP=21;I16=8,11,0,0,713,27117,0,0,1140,68400,0,0,371,8255,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,155:5:0 0,9,110:3:0 17 1042 . A 0 . DP=23;I16=12,10,0,0,800,29464,0,0,1297,76969,0,0,384,8466,0,0;QS=3,0;MQSB=0.947103;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,185:6:0 0,15,145:5:0 17 1043 . A 0 . DP=23;I16=12,11,0,0,850,32160,0,0,1357,80569,0,0,414,9192,0,0;QS=3,0;MQSB=0.944319;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,188:6:0 0,15,159:5:0 17 1044 . C 0 . DP=23;I16=12,10,0,0,812,30758,0,0,1297,76969,0,0,394,8690,0,0;QS=3,0;MQSB=0.947103;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,181:6:0 0,15,150:5:0 17 1045 . A 0 . DP=23;I16=12,11,0,0,887,34519,0,0,1357,80569,0,0,424,9460,0,0;QS=3,0;MQSB=0.944319;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,197:6:0 0,15,156:5:0 17 1046 . G 0 . DP=23;I16=12,11,0,0,862,33240,0,0,1357,80569,0,0,428,9576,0,0;QS=3,0;MQSB=0.944319;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,183:6:0 0,15,158:5:0 17 1047 . A 0 . DP=23;I16=12,11,0,0,890,34804,0,0,1357,80569,0,0,432,9712,0,0;QS=3,0;MQSB=0.944319;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,198:6:0 0,15,165:5:0 17 1048 . G 0 . DP=23;I16=12,10,0,0,846,33054,0,0,1297,76969,0,0,411,9243,0,0;QS=3,0;MQSB=0.947103;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,195:6:0 0,15,156:5:0 17 1049 . C 0 . DP=22;I16=12,10,0,0,808,30644,0,0,1297,76969,0,0,441,10043,0,0;QS=3,0;MQSB=0.947103;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,182:6:0 0,15,160:5:0 17 1050 . A 0 . DP=22;I16=11,10,0,0,808,31334,0,0,1237,73369,0,0,430,9940,0,0;QS=3,0;MQSB=0.939898;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,193:6:0 0,12,138:4:0 17 1051 . A 0 . DP=21;I16=11,9,0,0,731,27495,0,0,1177,69769,0,0,423,9621,0,0;QS=3,0;MQSB=0.943233;MQ0F=0 PL:DP:DV 0,27,238:9:0 0,18,187:6:0 0,15,150:5:0 17 1052 . A 0 . DP=21;I16=11,9,0,0,759,29327,0,0,1177,69769,0,0,427,9747,0,0;QS=3,0;MQSB=0.943233;MQ0F=0 PL:DP:DV 0,27,247:9:0 0,18,190:6:0 0,15,150:5:0 17 1053 . A 0 . DP=21;I16=11,8,0,0,674,25210,0,0,1117,66169,0,0,406,9264,0,0;QS=3,0;MQSB=0.946915;MQ0F=0 PL:DP:DV 0,24,224:8:0 0,18,161:6:0 0,15,149:5:0 17 1054 . C 0 . DP=21;I16=11,10,0,0,791,30157,0,0,1237,73369,0,0,459,10623,0,0;QS=3,0;MQSB=0.939898;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,189:6:0 0,15,153:5:0 17 1055 . C 0 . DP=22;I16=12,10,0,0,818,31448,0,0,1297,76969,0,0,462,10750,0,0;QS=3,0;MQSB=0.947103;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,191:6:0 0,15,157:5:0 17 1056 . C 0 . DP=22;I16=12,9,0,0,793,31265,0,0,1237,73369,0,0,441,10271,0,0;QS=3,0;MQSB=0.950149;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,195:6:0 0,15,161:5:0 17 1057 . T 0 . DP=23;I16=12,10,0,0,821,31667,0,0,1297,76969,0,0,467,10911,0,0;QS=3,0;MQSB=0.947103;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,196:6:0 0,15,159:5:0 17 1058 . G 0 . DP=23;I16=12,11,0,0,863,32871,0,0,1357,80569,0,0,493,11569,0,0;QS=3,0;MQSB=0.944319;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,21,197:7:0 0,15,155:5:0 17 1059 . T 0 . DP=23;I16=12,10,0,0,797,29803,0,0,1297,76969,0,0,468,10944,0,0;QS=3,0;MQSB=0.947103;MQ0F=0 PL:DP:DV 0,30,249:10:0 0,21,202:7:0 0,15,152:5:0 17 1060 . C 0 . DP=23;I16=12,11,0,0,829,31041,0,0,1357,80569,0,0,492,11536,0,0;QS=3,0;MQSB=0.944319;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,21,178:7:0 0,15,163:5:0 17 1061 . T 0 . DP=22;I16=12,9,0,0,810,31840,0,0,1237,73369,0,0,465,10797,0,0;QS=3,0;MQSB=0.950149;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,175:5:0 0,15,158:5:0 17 1062 . C A, 0 . DP=22;I16=12,9,0,1,826,32780,33,1089,1237,73369,60,3600,462,10652,25,625;QS=2.85398,0.146018,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.947103;BQB=1;MQ0F=0 PL:DP:DV 0,33,255,33,255,255:11:0 15,0,151,30,154,176:6:1 0,15,164,15,164,164:5:0 17 1063 . T 0 . DP=22;I16=12,9,0,0,820,32460,0,0,1237,73369,0,0,459,10525,0,0;QS=3,0;MQSB=0.950149;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,183:6:0 0,15,166:5:0 17 1064 . A 0 . DP=23;I16=12,9,0,0,793,30355,0,0,1237,73369,0,0,464,10752,0,0;QS=3,0;MQSB=0.950149;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,180:6:0 0,15,162:5:0 17 1065 . A 0 . DP=23;I16=12,9,0,0,814,31978,0,0,1237,73369,0,0,462,10694,0,0;QS=3,0;MQSB=0.950149;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,186:6:0 0,15,174:5:0 17 1066 . A 0 . DP=27;I16=15,9,0,0,890,34378,0,0,1417,84169,0,0,460,10652,0,0;QS=3,0;MQSB=0.96464;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,21,198:7:0 0,18,181:6:0 17 1067 . A 0 . DP=27;I16=15,10,0,0,913,35031,0,0,1477,87769,0,0,470,10600,0,0;QS=3,0;MQSB=0.962269;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,208:7:0 0,18,182:6:0 17 1068 . A 0 . DP=27;I16=15,9,0,0,898,34580,0,0,1417,84169,0,0,456,10342,0,0;QS=3,0;MQSB=0.96464;MQ0F=0 PL:DP:DV 0,30,253:10:0 0,24,218:8:0 0,18,181:6:0 17 1069 . A 0 . DP=28;I16=16,10,0,0,914,33888,0,0,1537,91369,0,0,481,10925,0,0;QS=3,0;MQSB=0.965874;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,207:8:0 0,21,177:7:0 17 1070 . A 0 . DP=28;I16=15,11,0,0,955,36445,0,0,1537,91369,0,0,467,10351,0,0;QS=3,0;MQSB=0.960078;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,223:8:0 0,21,188:7:0 17 1071 . G 0 . DP=28;I16=16,10,0,0,974,37570,0,0,1537,91369,0,0,466,10284,0,0;QS=3,0;MQSB=0.965874;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,192:7:0 0,21,191:7:0 17 1072 . A 0 . DP=28;I16=16,11,0,0,1007,38205,0,0,1597,94969,0,0,490,10868,0,0;QS=3,0;MQSB=0.96384;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,24,239:8:0 0,21,195:7:0 17 1073 . A 0 . DP=28;I16=16,10,0,0,976,37822,0,0,1537,91369,0,0,463,10177,0,0;QS=3,0;MQSB=0.965874;MQ0F=0 PL:DP:DV 0,33,254:11:0 0,24,236:8:0 0,21,199:7:0 17 1074 . A 0 . DP=28;I16=16,11,0,0,1003,38097,0,0,1597,94969,0,0,484,10664,0,0;QS=3,0;MQSB=0.96384;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,24,232:8:0 0,21,203:7:0 17 1075 . A 0 . DP=28;I16=16,10,0,0,964,36762,0,0,1537,91369,0,0,476,10564,0,0;QS=3,0;MQSB=0.965874;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,222:8:0 0,21,200:7:0 17 1076 . G 0 . DP=28;I16=16,10,0,0,966,37048,0,0,1537,91369,0,0,476,10536,0,0;QS=3,0;MQSB=0.965874;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,209:8:0 0,21,183:7:0 17 1077 . A 0 . DP=29;I16=17,11,0,0,1038,39240,0,0,1657,98569,0,0,480,10548,0,0;QS=3,0;MQSB=0.967085;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,24,231:8:0 0,24,209:8:0 17 1078 . A 0 . DP=29;I16=17,11,0,0,1053,40425,0,0,1657,98569,0,0,480,10562,0,0;QS=3,0;MQSB=0.967085;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,24,234:8:0 0,24,214:8:0 17 1079 . A 0 . DP=28;I16=17,10,0,0,1019,39007,0,0,1597,94969,0,0,479,10505,0,0;QS=3,0;MQSB=0.968979;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,223:8:0 0,24,206:8:0 17 1080 . A 0 . DP=29;I16=18,10,0,0,1049,40827,0,0,1657,98569,0,0,478,10478,0,0;QS=3,0;MQSB=0.971673;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,246:9:0 0,24,218:8:0 17 1081 . G 0 . DP=30;I16=19,8,0,0,988,37392,0,0,1597,94969,0,0,436,9550,0,0;QS=3,0;MQSB=0.977696;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,24,210:8:0 0,27,210:9:0 17 1082 . A 0 . DP=30;I16=20,7,0,0,989,37109,0,0,1597,94969,0,0,438,9564,0,0;QS=3,0;MQSB=0.981425;MQ0F=0 PL:DP:DV 0,27,224:9:0 0,27,226:9:0 0,27,217:9:0 17 1083 . A 0 . DP=30;I16=20,8,0,0,1039,39827,0,0,1657,98569,0,0,455,9803,0,0;QS=3,0;MQSB=0.979523;MQ0F=0 PL:DP:DV 0,30,246:10:0 0,27,233:9:0 0,27,217:9:0 17 1084 . A 0 . DP=31;I16=21,8,0,0,1049,39097,0,0,1717,102169,0,0,457,9849,0,0;QS=3,0;MQSB=0.981133;MQ0F=0 PL:DP:DV 0,33,253:11:0 0,27,226:9:0 0,27,215:9:0 17 1085 . A 0 . DP=30;I16=21,8,0,0,1052,39534,0,0,1717,102169,0,0,486,10552,0,0;QS=3,0;MQSB=0.981133;MQ0F=0 PL:DP:DV 0,30,232:10:0 0,30,247:10:0 0,27,214:9:0 17 1086 . A 0 . DP=28;I16=21,6,0,0,969,36223,0,0,1597,94969,0,0,492,10660,0,0;QS=3,0;MQSB=0.98481;MQ0F=0 PL:DP:DV 0,30,219:10:0 0,27,236:9:0 0,24,179:8:0 17 1087 . C 0 . DP=28;I16=22,6,0,0,984,36046,0,0,1657,98569,0,0,498,10796,0,0;QS=3,0;MQSB=0.985992;MQ0F=0 PL:DP:DV 0,30,234:10:0 0,27,229:9:0 0,27,173:9:0 17 1088 . T 0 . DP=29;I16=23,6,0,0,1133,45203,0,0,1717,102169,0,0,503,10863,0,0;QS=3,0;MQSB=0.987041;MQ0F=0 PL:DP:DV 0,30,252:10:0 0,30,250:10:0 0,27,198:9:0 17 1089 . C 0 . DP=29;I16=23,6,0,0,1093,42347,0,0,1717,102169,0,0,509,10965,0,0;QS=3,0;MQSB=0.987041;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,30,237:10:0 0,27,188:9:0 17 1090 . A 0 . DP=29;I16=23,6,0,0,1061,39845,0,0,1717,102169,0,0,515,11103,0,0;QS=3,0;MQSB=0.987041;MQ0F=0 PL:DP:DV 0,30,221:10:0 0,30,250:10:0 0,27,183:9:0 17 1091 . C 0 . DP=29;I16=23,6,0,0,1063,39713,0,0,1717,102169,0,0,521,11277,0,0;QS=3,0;MQSB=0.987041;MQ0F=0 PL:DP:DV 0,30,247:10:0 0,30,244:10:0 0,27,177:9:0 17 1092 . T 0 . DP=29;I16=23,6,0,0,1121,44489,0,0,1717,102169,0,0,524,11334,0,0;QS=3,0;MQSB=0.987041;MQ0F=0 PL:DP:DV 0,30,244:10:0 0,30,251:10:0 0,27,198:9:0 17 1093 . G 0 . DP=29;I16=23,6,0,0,1085,41377,0,0,1717,102169,0,0,526,11372,0,0;QS=3,0;MQSB=0.987041;MQ0F=0 PL:DP:DV 0,30,249:10:0 0,30,243:10:0 0,27,184:9:0 17 1094 . G 0 . DP=29;I16=22,6,0,0,1018,38808,0,0,1657,98569,0,0,521,11393,0,0;QS=3,0;MQSB=0.985992;MQ0F=0 PL:DP:DV 0,30,243:10:0 0,30,232:10:0 0,24,185:8:0 17 1095 . A 0 . DP=28;I16=22,6,0,0,1023,38551,0,0,1657,98569,0,0,529,11443,0,0;QS=3,0;MQSB=0.985992;MQ0F=0 PL:DP:DV 0,30,236:10:0 0,30,244:10:0 0,24,178:8:0 17 1096 . T 0 . DP=30;I16=24,5,0,0,1020,37168,0,0,1717,102169,0,0,505,10849,0,0;QS=3,0;MQSB=0.989637;MQ0F=0 PL:DP:DV 0,33,234:11:0 0,27,220:9:0 0,27,178:9:0 17 1097 . A 0 . DP=30;I16=24,6,0,0,1056,38166,0,0,1777,105769,0,0,533,11537,0,0;QS=3,0;MQSB=0.987976;MQ0F=0 PL:DP:DV 0,33,230:11:0 0,30,237:10:0 0,27,177:9:0 17 1098 . T 0 . DP=29;I16=24,5,0,0,1081,40557,0,0,1717,102169,0,0,537,11633,0,0;QS=3,0;MQSB=0.989637;MQ0F=0 PL:DP:DV 0,30,210:10:0 0,30,251:10:0 0,27,186:9:0 17 1099 . G 0 . DP=29;I16=24,5,0,0,1091,41719,0,0,1717,102169,0,0,540,11712,0,0;QS=3,0;MQSB=0.989637;MQ0F=0 PL:DP:DV 0,30,229:10:0 0,30,242:10:0 0,27,182:9:0 17 1100 . A 0 . DP=29;I16=24,5,0,0,1106,42976,0,0,1717,102169,0,0,543,11825,0,0;QS=3,0;MQSB=0.989637;MQ0F=0 PL:DP:DV 0,30,210:10:0 0,30,255:10:0 0,27,191:9:0 17 1101 . A 0 . DP=29;I16=24,5,0,0,1154,46420,0,0,1717,102169,0,0,545,11921,0,0;QS=3,0;MQSB=0.989637;MQ0F=0 PL:DP:DV 0,30,222:10:0 0,30,255:10:0 0,27,198:9:0 17 1102 . T 0 . DP=29;I16=24,4,0,0,1046,39520,0,0,1657,98569,0,0,522,11424,0,0;QS=3,0;MQSB=0.991416;MQ0F=0 PL:DP:DV 0,30,210:10:0 0,27,233:9:0 0,27,186:9:0 17 1103 . G 0 . DP=30;I16=24,6,0,0,1134,43486,0,0,1777,105769,0,0,548,12158,0,0;QS=3,0;MQSB=0.987976;MQ0F=0 PL:DP:DV 0,30,223:10:0 0,30,255:10:0 0,30,222:10:0 17 1104 . A 0 . DP=28;I16=23,5,0,0,1089,43067,0,0,1657,98569,0,0,552,12296,0,0;QS=3,0;MQSB=0.988819;MQ0F=0 PL:DP:DV 0,27,213:9:0 0,27,233:9:0 0,30,233:10:0 17 1105 . T 0 . DP=28;I16=23,5,0,0,1035,38743,0,0,1657,98569,0,0,556,12462,0,0;QS=3,0;MQSB=0.988819;MQ0F=0 PL:DP:DV 0,27,203:9:0 0,27,227:9:0 0,30,222:10:0 17 1106 . A 0 . DP=29;I16=23,5,0,0,1003,36715,0,0,1657,98569,0,0,532,11882,0,0;QS=3,0;MQSB=0.988819;MQ0F=0 PL:DP:DV 0,30,227:10:0 0,24,198:8:0 0,30,217:10:0 17 1107 . C 0 . DP=29;I16=23,6,0,0,1093,41509,0,0,1717,102169,0,0,558,12532,0,0;QS=3,0;MQSB=0.987041;MQ0F=0 PL:DP:DV 0,30,244:10:0 0,27,233:9:0 0,30,220:10:0 17 1108 . A 0 . DP=29;I16=23,6,0,0,1138,44908,0,0,1717,102169,0,0,558,12536,0,0;QS=3,0;MQSB=0.987041;MQ0F=0 PL:DP:DV 0,30,239:10:0 0,27,253:9:0 0,30,227:10:0 17 1109 . G 0 . DP=29;I16=23,6,0,0,1140,45200,0,0,1717,102169,0,0,557,12519,0,0;QS=3,0;MQSB=0.987041;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,27,240:9:0 0,30,224:10:0 17 1110 . G 0 . DP=29;I16=23,6,0,0,1086,41054,0,0,1717,102169,0,0,555,12481,0,0;QS=3,0;MQSB=0.987041;MQ0F=0 PL:DP:DV 0,30,242:10:0 0,27,229:9:0 0,30,224:10:0 17 1111 . T 0 . DP=29;I16=22,6,0,0,1025,37919,0,0,1680,100800,0,0,552,12470,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,227:10:0 0,24,227:8:0 0,30,211:10:0 17 1112 . T 0 . DP=28;I16=22,6,0,0,1034,38986,0,0,1680,100800,0,0,550,12440,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,226:10:0 0,24,221:8:0 0,30,221:10:0 17 1113 . G 0 . DP=28;I16=23,5,0,0,1085,42273,0,0,1680,100800,0,0,548,12386,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,202:7:0 0,33,222:11:0 17 1114 . A 0 . DP=28;I16=23,5,0,0,1079,42279,0,0,1680,100800,0,0,546,12306,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,235:10:0 0,21,209:7:0 0,33,238:11:0 17 1115 . G 0 . DP=28;I16=23,5,0,0,1114,44550,0,0,1680,100800,0,0,544,12250,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,208:7:0 0,33,234:11:0 17 1116 . G 0 . DP=29;I16=24,5,0,0,1059,39531,0,0,1740,104400,0,0,542,12218,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,236:10:0 0,24,204:8:0 0,33,226:11:0 17 1117 . A 0 . DP=29;I16=24,5,0,0,1124,43896,0,0,1740,104400,0,0,541,12211,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,235:10:0 0,24,227:8:0 0,33,234:11:0 17 1118 . T 0 . DP=29;I16=24,4,0,0,1100,44802,0,0,1680,100800,0,0,539,12131,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,230:10:0 0,21,188:7:0 0,33,248:11:0 17 1119 . C 0 . DP=29;I16=23,4,0,0,1078,44864,0,0,1620,97200,0,0,526,11958,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,224:9:0 0,21,191:7:0 0,33,255:11:0 17 1120 . C 0 . DP=29;I16=23,5,0,0,1113,46071,0,0,1680,100800,0,0,536,12054,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,227:9:0 0,24,214:8:0 0,33,255:11:0 17 1121 . A 0 . DP=30;I16=24,5,0,0,1167,48549,0,0,1740,104400,0,0,536,12056,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,233:9:0 0,24,220:8:0 0,36,255:12:0 17 1122 . T 0 . DP=32;I16=26,5,0,0,1235,50967,0,0,1860,111600,0,0,535,11985,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,230:10:0 0,27,238:9:0 0,36,255:12:0 17 1123 . T 0 . DP=32;I16=26,5,0,0,1219,50121,0,0,1860,111600,0,0,535,11893,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,221:10:0 0,27,236:9:0 0,36,255:12:0 17 1124 . A 0 . DP=31;I16=25,5,0,0,1187,48827,0,0,1800,108000,0,0,536,11832,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,218:9:0 0,27,235:9:0 0,36,255:12:0 17 1125 . T 0 . DP=31;I16=25,5,0,0,1211,51001,0,0,1800,108000,0,0,537,11801,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,228:9:0 0,27,235:9:0 0,36,255:12:0 17 1126 . C 0 . DP=31;I16=25,5,0,0,1225,52001,0,0,1800,108000,0,0,538,11800,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,234:9:0 0,27,241:9:0 0,36,255:12:0 17 1127 . T 0 . DP=31;I16=25,5,0,0,1257,55245,0,0,1800,108000,0,0,539,11829,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,231:9:0 0,27,252:9:0 0,36,255:12:0 17 1128 . G 0 . DP=31;I16=25,5,0,0,1217,51743,0,0,1800,108000,0,0,540,11888,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,236:9:0 0,27,229:9:0 0,36,255:12:0 17 1129 . A G, 0 . DP=31;I16=24,4,0,1,1101,45631,32,1024,1680,100800,60,3600,514,11300,25,625;QS=2.93535,0.0646465,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0 PL:DP:DV 0,24,204,24,204,204:8:0 0,27,229,27,229,229:9:0 0,4,198,33,201,219:12:1 17 1130 . A 0 . DP=31;I16=25,5,0,0,1189,49509,0,0,1800,108000,0,0,539,11943,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,215:9:0 0,27,236:9:0 0,36,255:12:0 17 1131 . A 0 . DP=29;I16=23,5,0,0,1156,49362,0,0,1680,100800,0,0,540,11988,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,206:7:0 0,27,250:9:0 0,36,255:12:0 17 1132 . T 0 . DP=29;I16=23,5,0,0,1123,47359,0,0,1680,100800,0,0,540,12008,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,198:7:0 0,27,229:9:0 0,36,255:12:0 17 1133 . G 0 . DP=30;I16=23,5,0,0,1148,48796,0,0,1680,100800,0,0,540,12052,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,214:7:0 0,27,232:9:0 0,36,255:12:0 17 1134 . C 0 . DP=29;I16=22,6,0,0,1162,50224,0,0,1680,100800,0,0,547,12155,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,209:7:0 0,27,254:9:0 0,36,255:12:0 17 1135 . T 0 . DP=29;I16=22,6,0,0,1214,55020,0,0,1680,100800,0,0,549,12257,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,209:7:0 0,27,255:9:0 0,36,255:12:0 17 1136 . T 0 . DP=29;I16=22,6,0,0,1148,49270,0,0,1680,100800,0,0,551,12383,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,200:7:0 0,27,255:9:0 0,36,255:12:0 17 1137 . G 0 . DP=28;I16=21,6,0,0,1087,46051,0,0,1620,97200,0,0,554,12532,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,214:7:0 0,27,234:9:0 0,33,254:11:0 17 1138 . G 0 . DP=28;I16=21,6,0,0,1040,42504,0,0,1620,97200,0,0,557,12703,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,197:7:0 0,27,234:9:0 0,33,246:11:0 17 1139 . A 0 . DP=28;I16=21,6,0,0,1103,47389,0,0,1620,97200,0,0,559,12845,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,200:7:0 0,27,254:9:0 0,33,255:11:0 17 1140 . C 0 . DP=28;I16=21,6,0,0,1072,44372,0,0,1620,97200,0,0,561,13007,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,214:7:0 0,27,234:9:0 0,33,250:11:0 17 1141 . C 0 . DP=28;I16=21,6,0,0,1106,47298,0,0,1620,97200,0,0,562,13140,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,212:7:0 0,27,245:9:0 0,33,255:11:0 17 1142 . A 0 . DP=28;I16=21,6,0,0,1114,47788,0,0,1620,97200,0,0,560,13146,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,212:7:0 0,27,255:9:0 0,33,251:11:0 17 1143 . G 0 . DP=26;I16=19,7,0,0,1041,42249,0,0,1560,93600,0,0,585,13799,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,222:7:0 0,27,249:9:0 0,30,253:10:0 17 1144 . A 0 . DP=26;I16=19,7,0,0,1018,40154,0,0,1560,93600,0,0,585,13847,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,205:7:0 0,27,250:9:0 0,30,255:10:0 17 1145 . T 0 . DP=26;I16=19,7,0,0,1016,39852,0,0,1560,93600,0,0,584,13866,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,206:7:0 0,27,250:9:0 0,30,249:10:0 17 1146 . G 0 . DP=26;I16=19,7,0,0,1011,39743,0,0,1560,93600,0,0,582,13856,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,210:7:0 0,27,246:9:0 0,30,254:10:0 17 1147 . T 0 . DP=27;I16=20,6,0,0,1010,39730,0,0,1560,93600,0,0,579,13815,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,207:8:0 0,24,234:8:0 0,30,255:10:0 17 1148 . T 0 . DP=26;I16=20,6,0,0,1002,39214,0,0,1560,93600,0,0,576,13690,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,196:8:0 0,24,237:8:0 0,30,255:10:0 17 1149 . T 0 . DP=26;I16=20,6,0,0,1022,40532,0,0,1560,93600,0,0,573,13579,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,205:8:0 0,24,231:8:0 0,30,255:10:0 17 1150 . T 0 . DP=26;I16=20,6,0,0,1032,41212,0,0,1560,93600,0,0,569,13433,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,208:8:0 0,24,236:8:0 0,30,255:10:0 17 1151 . G 0 . DP=26;I16=20,6,0,0,1021,40285,0,0,1560,93600,0,0,565,13303,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,220:8:0 0,24,231:8:0 0,30,248:10:0 17 1152 . A 0 . DP=27;I16=20,7,0,0,1040,40772,0,0,1620,97200,0,0,561,13189,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,202:8:0 0,24,230:8:0 0,33,255:11:0 17 1153 . A 0 . DP=27;I16=20,7,0,0,1039,40717,0,0,1620,97200,0,0,557,13043,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,202:8:0 0,24,233:8:0 0,33,255:11:0 17 1154 . T 0 . DP=28;I16=21,7,0,0,1073,41861,0,0,1680,100800,0,0,552,12866,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,212:9:0 0,24,236:8:0 0,33,255:11:0 17 1155 . T 0 . DP=27;I16=20,7,0,0,1074,43046,0,0,1620,97200,0,0,549,12707,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,211:8:0 0,24,236:8:0 0,33,255:11:0 17 1156 . T 0 . DP=28;I16=19,8,0,0,1065,42533,0,0,1620,97200,0,0,520,11892,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,210:8:0 0,24,239:8:0 0,33,255:11:0 17 1157 . T 0 . DP=28;I16=20,8,0,0,1094,43108,0,0,1680,100800,0,0,541,12299,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,230:9:0 0,24,230:8:0 0,33,255:11:0 17 1158 . G 0 . DP=28;I16=20,8,0,0,1098,43584,0,0,1680,100800,0,0,536,12056,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,240:9:0 0,24,224:8:0 0,33,255:11:0 17 1159 . G 0 . DP=28;I16=20,8,0,0,1096,43222,0,0,1680,100800,0,0,530,11790,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,249:9:0 0,24,229:8:0 0,33,255:11:0 17 1160 . A 0 . DP=30;I16=20,10,0,0,1146,44510,0,0,1800,108000,0,0,524,11552,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,229:9:0 0,24,242:8:0 0,39,255:13:0 17 1161 . T 0 . DP=30;I16=20,10,0,0,1136,43548,0,0,1800,108000,0,0,520,11344,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,221:9:0 0,24,230:8:0 0,39,255:13:0 17 1162 . T 0 . DP=30;I16=20,10,0,0,1147,44365,0,0,1800,108000,0,0,516,11168,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,232:9:0 0,24,229:8:0 0,39,255:13:0 17 1163 . T 0 . DP=31;I16=20,11,0,0,1176,45394,0,0,1860,111600,0,0,511,10975,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,243:10:0 0,24,231:8:0 0,39,255:13:0 17 1164 . T 0 . DP=31;I16=20,11,0,0,1166,44864,0,0,1860,111600,0,0,506,10768,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,235:10:0 0,24,234:8:0 0,39,255:13:0 17 1165 . T 0 . DP=31;I16=20,11,0,0,1189,46635,0,0,1860,111600,0,0,501,10599,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,240:10:0 0,24,231:8:0 0,39,255:13:0 17 1166 . T 0 . DP=30;I16=19,11,0,0,1137,43791,0,0,1800,108000,0,0,497,10467,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,231:9:0 0,24,228:8:0 0,39,255:13:0 17 1167 . C 0 . DP=28;I16=17,11,0,0,1114,44588,0,0,1680,100800,0,0,495,10369,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,21,216:7:0 0,36,255:12:0 17 1168 . A 0 . DP=28;I16=17,11,0,0,1074,41862,0,0,1680,100800,0,0,493,10303,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,238:9:0 0,21,203:7:0 0,36,255:12:0 17 1169 . T 0 . DP=28;I16=17,11,0,0,1089,42589,0,0,1680,100800,0,0,491,10269,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,247:9:0 0,21,212:7:0 0,36,255:12:0 17 1170 . A 0 . DP=27;I16=16,11,0,0,1033,39891,0,0,1620,97200,0,0,490,10266,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,244:9:0 0,21,214:7:0 0,33,255:11:0 17 1171 . T 0 . DP=28;I16=17,11,0,0,1086,42472,0,0,1680,100800,0,0,488,10244,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,249:9:0 0,24,223:8:0 0,33,255:11:0 17 1172 . T 0 . DP=28;I16=17,11,0,0,1086,42598,0,0,1680,100800,0,0,486,10206,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,235:9:0 0,24,228:8:0 0,33,255:11:0 17 1173 . T 0 . DP=29;I16=18,11,0,0,1126,44348,0,0,1740,104400,0,0,483,10153,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,241:9:0 0,27,241:9:0 0,33,255:11:0 17 1174 . T 0 . DP=29;I16=18,11,0,0,1098,42352,0,0,1740,104400,0,0,481,10135,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,230:9:0 0,27,238:9:0 0,33,255:11:0 17 1175 . G 0 . DP=28;I16=18,10,0,0,1063,40975,0,0,1680,100800,0,0,480,10152,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,234:8:0 0,27,234:9:0 0,33,255:11:0 17 1176 . T 0 . DP=28;I16=18,10,0,0,1045,39823,0,0,1680,100800,0,0,479,10203,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,221:8:0 0,27,233:9:0 0,33,255:11:0 17 1177 . A 0 . DP=28;I16=18,10,0,0,1040,39006,0,0,1680,100800,0,0,478,10288,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,220:8:0 0,27,228:9:0 0,33,255:11:0 17 1178 . A 0 . DP=27;I16=17,10,0,0,1006,38238,0,0,1620,97200,0,0,477,10355,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,221:8:0 0,27,227:9:0 0,30,255:10:0 17 1179 . T 0 . DP=27;I16=17,10,0,0,1012,38396,0,0,1620,97200,0,0,475,10403,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,223:8:0 0,27,224:9:0 0,30,255:10:0 17 1180 . C 0 . DP=27;I16=16,10,0,0,1006,39508,0,0,1560,93600,0,0,469,10423,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,222:7:0 0,27,224:9:0 0,30,255:10:0 17 1181 . T 0 . DP=26;I16=16,10,0,0,1021,40581,0,0,1560,93600,0,0,469,10441,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,236:8:0 0,24,229:8:0 0,30,255:10:0 17 1182 . T 0 . DP=26;I16=14,11,0,0,939,35915,0,0,1500,90000,0,0,457,10347,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,236:9:0 0,21,209:7:0 0,27,255:9:0 17 1183 . T 0 . DP=25;I16=13,11,0,0,899,34555,0,0,1440,86400,0,0,455,10341,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,239:9:0 0,18,192:6:0 0,27,255:9:0 17 1184 . G 0 . DP=24;I16=13,11,0,0,916,35398,0,0,1440,86400,0,0,465,10479,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,234:8:0 0,21,201:7:0 0,27,255:9:0 17 1185 . C 0 . DP=24;I16=13,11,0,0,908,35014,0,0,1440,86400,0,0,465,10541,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,246:8:0 0,21,191:7:0 0,27,250:9:0 17 1186 . A 0 . DP=24;I16=13,11,0,0,946,37648,0,0,1440,86400,0,0,463,10525,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,240:8:0 0,21,205:7:0 0,27,255:9:0 17 1187 . G 0 . DP=25;I16=14,11,0,0,908,33870,0,0,1500,90000,0,0,461,10529,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,240:9:0 0,21,205:7:0 0,27,240:9:0 17 1188 . T 0 . DP=25;I16=14,11,0,0,886,32138,0,0,1500,90000,0,0,461,10553,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,248:10:0 0,21,196:7:0 0,24,216:8:0 17 1189 . A 0 . DP=25;I16=14,11,0,0,920,34392,0,0,1500,90000,0,0,461,10497,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,174:6:0 0,27,252:9:0 17 1190 . T 0 . DP=26;I16=14,12,0,0,960,35876,0,0,1560,93600,0,0,462,10462,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,185:6:0 0,27,252:9:0 17 1191 . A 0 . DP=26;I16=14,12,0,0,928,33922,0,0,1560,93600,0,0,464,10450,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,173:6:0 0,27,245:9:0 17 1192 . T 0 . DP=26;I16=14,12,0,0,981,37505,0,0,1560,93600,0,0,465,10413,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,191:6:0 0,27,255:9:0 17 1193 . T 0 . DP=26;I16=14,12,0,0,976,37054,0,0,1560,93600,0,0,466,10402,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,191:6:0 0,27,255:9:0 17 1194 . T 0 . DP=26;I16=14,12,0,0,951,35219,0,0,1560,93600,0,0,466,10368,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,178:6:0 0,27,249:9:0 17 1195 . A 0 . DP=26;I16=14,12,0,0,917,33253,0,0,1560,93600,0,0,466,10362,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,177:6:0 0,27,235:9:0 17 1196 . C 0 . DP=25;I16=13,12,0,0,921,34209,0,0,1500,90000,0,0,466,10334,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,187:6:0 0,27,236:9:0 17 1197 . C 0 . DP=24;I16=12,12,0,0,906,34862,0,0,1440,86400,0,0,464,10184,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,186:6:0 0,24,233:8:0 17 1198 . A 0 . DP=24;I16=12,12,0,0,937,36815,0,0,1440,86400,0,0,461,10013,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,190:6:0 0,24,245:8:0 17 1199 . G 0 . DP=24;I16=12,12,0,0,879,33035,0,0,1440,86400,0,0,457,9821,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,163:6:0 0,24,221:8:0 17 1200 . T 0 . DP=24;I16=11,12,0,0,895,35141,0,0,1380,82800,0,0,428,9032,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,176:5:0 0,24,247:8:0 17 1201 . T 0 . DP=24;I16=12,12,0,0,889,33727,0,0,1440,86400,0,0,449,9521,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,177:6:0 0,24,238:8:0 17 1202 . C 0 . DP=25;I16=13,12,0,0,929,35121,0,0,1500,90000,0,0,445,9413,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,175:6:0 0,24,230:8:0 17 1203 . A 0 . DP=25;I16=13,12,0,0,943,36107,0,0,1500,90000,0,0,442,9334,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,184:6:0 0,24,241:8:0 17 1204 . G 0 . DP=24;I16=13,11,0,0,876,33106,0,0,1440,86400,0,0,439,9235,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,174:6:0 0,21,218:7:0 17 1205 . C 0 . DP=24;I16=13,11,0,0,891,33869,0,0,1440,86400,0,0,436,9166,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,176:6:0 0,21,217:7:0 17 1206 . A 0 . DP=23;I16=13,10,0,0,846,31744,0,0,1380,82800,0,0,434,9126,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,179:6:0 0,21,212:7:0 17 1207 . T 0 . DP=23;I16=13,9,0,0,819,30877,0,0,1320,79200,0,0,407,8489,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,254:10:0 0,18,189:6:0 0,18,194:6:0 17 1208 . C 0 . DP=24;I16=14,10,0,0,910,35236,0,0,1440,86400,0,0,429,9079,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,190:7:0 0,21,217:7:0 17 1209 . C T, 0 . DP=24;I16=14,9,0,1,869,33481,21,441,1380,82800,60,3600,408,8710,19,361;QS=2.91393,0.0860656,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0 PL:DP:DV 0,30,255,30,255,255:10:0 0,0,153,18,156,166:7:1 0,21,209,21,209,209:7:0 17 1210 . C 0 . DP=24;I16=14,10,0,0,915,35713,0,0,1440,86400,0,0,425,9091,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,199:7:0 0,21,209:7:0 17 1211 . T 0 . DP=24;I16=14,10,0,0,905,34669,0,0,1440,86400,0,0,423,9139,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,213:7:0 0,21,214:7:0 17 1212 . A 0 . DP=24;I16=14,10,0,0,850,30690,0,0,1440,86400,0,0,421,9215,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,246:10:0 0,21,190:7:0 0,21,204:7:0 17 1213 . A 0 . DP=24;I16=14,10,0,0,852,31192,0,0,1440,86400,0,0,418,9268,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,253:10:0 0,21,197:7:0 0,21,195:7:0 17 1214 . C 0 . DP=23;I16=13,10,0,0,851,32099,0,0,1380,82800,0,0,415,9295,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,186:7:0 0,18,184:6:0 17 1215 . T 0 . DP=23;I16=13,9,0,0,839,32475,0,0,1320,79200,0,0,386,8668,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,211:7:0 0,15,172:5:0 17 1216 . C 0 . DP=23;I16=13,10,0,0,863,32923,0,0,1380,82800,0,0,406,9260,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,196:7:0 0,18,172:6:0 17 1217 . A 0 . DP=22;I16=12,10,0,0,820,30926,0,0,1320,79200,0,0,402,9244,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,172:6:0 0,18,191:6:0 17 1218 . A 0 . DP=22;I16=12,10,0,0,764,27286,0,0,1320,79200,0,0,398,9244,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,145:6:0 0,18,184:6:0 17 1219 . A 0 . DP=21;I16=12,9,0,0,803,31119,0,0,1260,75600,0,0,395,9259,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,175:6:0 0,15,173:5:0 17 1220 . A 0 . DP=21;I16=12,9,0,0,752,27700,0,0,1260,75600,0,0,392,9288,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,253:10:0 0,18,167:6:0 0,15,167:5:0 17 1221 . A 0 . DP=20;I16=12,8,0,0,722,26564,0,0,1200,72000,0,0,390,9330,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,147:5:0 0,15,157:5:0 17 1222 . T 0 . DP=18;I16=10,8,0,0,666,25034,0,0,1080,64800,0,0,389,9333,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,245:9:0 0,15,143:5:0 0,12,142:4:0 17 1223 . T 0 . DP=17;I16=9,7,0,0,574,21262,0,0,960,57600,0,0,364,8720,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,232:9:0 0,12,122:4:0 0,9,120:3:0 17 1224 . C 0 . DP=19;I16=10,7,0,0,639,24429,0,0,1020,61200,0,0,364,8740,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,128:4:0 0,9,112:3:0 17 1225 . A 0 . DP=19;I16=10,9,0,0,713,27139,0,0,1109,65641,0,0,395,9419,0,0;QS=3,0;MQSB=0.920044;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,137:5:0 0,12,145:4:0 17 1226 . A 0 . DP=19;I16=10,9,0,0,688,25468,0,0,1109,65641,0,0,397,9469,0,0;QS=3,0;MQSB=0.920044;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,127:5:0 0,12,147:4:0 17 1227 . A 0 . DP=19;I16=10,9,0,0,698,26104,0,0,1109,65641,0,0,399,9531,0,0;QS=3,0;MQSB=0.920044;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,133:5:0 0,12,146:4:0 17 1228 . A 0 . DP=19;I16=10,9,0,0,705,26721,0,0,1109,65641,0,0,399,9505,0,0;QS=3,0;MQSB=0.920044;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,143:5:0 0,12,145:4:0 17 1229 . A 0 . DP=18;I16=10,8,0,0,693,26891,0,0,1049,62041,0,0,400,9490,0,0;QS=3,0;MQSB=0.906029;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,123:4:0 0,12,138:4:0 17 1230 . T 0 . DP=18;I16=10,8,0,0,643,23601,0,0,1049,62041,0,0,401,9485,0,0;QS=3,0;MQSB=0.906029;MQ0F=0 PL:DP:DV 0,30,245:10:0 0,12,115:4:0 0,12,144:4:0 17 1231 . C 0 . DP=18;I16=10,7,0,0,663,26041,0,0,1020,61200,0,0,390,9320,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,98:3:0 0,12,138:4:0 17 1232 . T 0 . DP=18;I16=10,8,0,0,683,26261,0,0,1049,62041,0,0,401,9409,0,0;QS=3,0;MQSB=0.906029;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,133:4:0 0,12,129:4:0 17 1233 . G 0 . DP=18;I16=10,8,0,0,657,24509,0,0,1049,62041,0,0,401,9389,0,0;QS=3,0;MQSB=0.906029;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,124:4:0 0,12,129:4:0 17 1234 . A 0 . DP=19;I16=10,8,0,0,677,26177,0,0,1080,64800,0,0,386,9134,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,101:3:0 0,12,144:4:0 17 1235 . A 0 . DP=19;I16=10,9,0,0,697,26363,0,0,1109,65641,0,0,400,9282,0,0;QS=3,0;MQSB=0.920044;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,129:4:0 0,12,140:4:0 17 1236 . A 0 . DP=19;I16=10,9,0,0,671,24693,0,0,1109,65641,0,0,398,9148,0,0;QS=3,0;MQSB=0.920044;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,103:4:0 0,12,136:4:0 17 1237 . T 0 . DP=19;I16=9,9,0,0,649,24061,0,0,1049,62041,0,0,370,8356,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,98:3:0 0,12,135:4:0 17 1238 . C 0 . DP=20;I16=11,9,0,0,733,27709,0,0,1169,69241,0,0,391,8783,0,0;QS=3,0;MQSB=0.916401;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,104:4:0 0,12,145:4:0 17 1239 . C 0 . DP=20;I16=10,9,0,0,672,24596,0,0,1109,65641,0,0,363,7981,0,0;QS=3,0;MQSB=0.920044;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,76:3:0 0,12,124:4:0 17 1240 . C 0 . DP=20;I16=11,9,0,0,723,26895,0,0,1169,69241,0,0,385,8451,0,0;QS=3,0;MQSB=0.916401;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,118:4:0 0,12,129:4:0 17 1241 . A 0 . DP=20;I16=11,9,0,0,719,26575,0,0,1169,69241,0,0,382,8318,0,0;QS=3,0;MQSB=0.916401;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,104:4:0 0,12,141:4:0 17 1242 . A 0 . DP=21;I16=12,9,0,0,749,27599,0,0,1229,72841,0,0,379,8207,0,0;QS=3,0;MQSB=0.913101;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,109:4:0 0,15,153:5:0 17 1243 . A 0 . DP=21;I16=12,9,0,0,738,26862,0,0,1229,72841,0,0,377,8119,0,0;QS=3,0;MQSB=0.913101;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,110:4:0 0,15,158:5:0 17 1244 . C 0 . DP=21;I16=12,8,0,0,670,22952,0,0,1169,69241,0,0,365,7955,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,101:4:0 0,15,148:5:0 17 1245 . G 0 . DP=21;I16=12,9,0,0,657,21627,0,0,1229,72841,0,0,373,8015,0,0;QS=3,0;MQSB=0.913101;MQ0F=0 PL:DP:DV 0,36,245:12:0 0,12,95:4:0 0,15,158:5:0 17 1246 . C 0 . DP=21;I16=12,9,0,0,652,21348,0,0,1229,72841,0,0,370,7948,0,0;QS=3,0;MQSB=0.913101;MQ0F=0 PL:DP:DV 0,36,252:12:0 0,12,95:4:0 0,15,146:5:0 17 1247 . G 0 . DP=20;I16=11,9,0,0,655,22291,0,0,1169,69241,0,0,367,7853,0,0;QS=3,0;MQSB=0.916401;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,87:4:0 0,15,150:5:0 17 1248 . C 0 . DP=20;I16=10,8,0,0,659,25037,0,0,1080,64800,0,0,314,6530,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,90:3:0 0,15,156:5:0 17 1249 . C 0 . DP=21;I16=12,9,0,0,772,28954,0,0,1229,72841,0,0,360,7680,0,0;QS=3,0;MQSB=0.913101;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,111:4:0 0,15,166:5:0 17 1250 . A 0 . DP=21;I16=12,9,0,0,757,27599,0,0,1229,72841,0,0,356,7554,0,0;QS=3,0;MQSB=0.913101;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,114:4:0 0,15,161:5:0 17 1251 . A 0 . DP=21;I16=12,9,0,0,758,27890,0,0,1229,72841,0,0,352,7452,0,0;QS=3,0;MQSB=0.913101;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,115:4:0 0,15,174:5:0 17 1252 . T 0 . DP=21;I16=12,9,0,0,758,27574,0,0,1229,72841,0,0,348,7374,0,0;QS=3,0;MQSB=0.913101;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,124:4:0 0,15,159:5:0 17 1253 . A 0 . DP=20;I16=12,8,0,0,718,26292,0,0,1169,69241,0,0,345,7319,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,127:4:0 0,12,144:4:0 17 1254 . A 0 . DP=20;I16=12,8,0,0,781,30817,0,0,1169,69241,0,0,342,7286,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,134:4:0 0,12,156:4:0 17 1255 . G 0 . DP=21;I16=12,9,0,0,785,30039,0,0,1229,72841,0,0,339,7275,0,0;QS=3,0;MQSB=0.913101;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,128:4:0 0,15,169:5:0 17 1256 . C 0 . DP=20;I16=12,8,0,0,742,27910,0,0,1169,69241,0,0,338,7286,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,116:4:0 0,15,165:5:0 17 1257 . A 0 . DP=20;I16=12,8,0,0,745,28017,0,0,1169,69241,0,0,337,7319,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,132:4:0 0,15,167:5:0 17 1258 . T 0 . DP=20;I16=12,8,0,0,753,28507,0,0,1169,69241,0,0,336,7374,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,126:4:0 0,15,170:5:0 17 1259 . T 0 . DP=20;I16=12,8,0,0,690,24762,0,0,1169,69241,0,0,335,7451,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,33,236:11:0 0,12,128:4:0 0,15,170:5:0 17 1260 . C 0 . DP=20;I16=12,8,0,0,719,26541,0,0,1169,69241,0,0,333,7499,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,127:4:0 0,15,155:5:0 17 1261 . C 0 . DP=18;I16=11,6,0,0,602,22374,0,0,989,58441,0,0,308,6940,0,0;QS=3,0;MQSB=0.85832;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,117:4:0 0,9,109:3:0 17 1262 . C 0 . DP=18;I16=12,6,0,0,653,24345,0,0,1049,62041,0,0,333,7597,0,0;QS=3,0;MQSB=0.85394;MQ0F=0 PL:DP:DV 0,30,253:10:0 0,15,145:5:0 0,9,114:3:0 17 1263 . T 0 . DP=17;I16=12,5,0,0,654,25656,0,0,989,58441,0,0,335,7645,0,0;QS=3,0;MQSB=0.818731;MQ0F=0 PL:DP:DV 0,27,238:9:0 0,15,158:5:0 0,9,118:3:0 17 1264 . T 0 . DP=17;I16=12,5,0,0,615,22855,0,0,989,58441,0,0,336,7658,0,0;QS=3,0;MQSB=0.818731;MQ0F=0 PL:DP:DV 0,27,226:9:0 0,15,149:5:0 0,9,114:3:0 17 1265 . T 0 . DP=17;I16=12,5,0,0,620,23176,0,0,989,58441,0,0,334,7538,0,0;QS=3,0;MQSB=0.818731;MQ0F=0 PL:DP:DV 0,27,232:9:0 0,15,144:5:0 0,9,114:3:0 17 1266 . G 0 . DP=18;I16=13,5,0,0,675,25765,0,0,1049,62041,0,0,332,7438,0,0;QS=3,0;MQSB=0.814433;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,145:5:0 0,9,114:3:0 17 1267 . A 0 . DP=18;I16=13,5,0,0,663,24799,0,0,1049,62041,0,0,331,7359,0,0;QS=3,0;MQSB=0.814433;MQ0F=0 PL:DP:DV 0,30,253:10:0 0,15,138:5:0 0,9,114:3:0 17 1268 . G 0 . DP=18;I16=13,5,0,0,638,23584,0,0,1049,62041,0,0,329,7251,0,0;QS=3,0;MQSB=0.814433;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,112:5:0 0,9,108:3:0 17 1269 . C 0 . DP=18;I16=13,5,0,0,592,20458,0,0,1049,62041,0,0,327,7163,0,0;QS=3,0;MQSB=0.814433;MQ0F=0 PL:DP:DV 0,30,224:10:0 0,15,130:5:0 0,9,107:3:0 17 1270 . G 0 . DP=18;I16=13,5,0,0,537,16775,0,0,1049,62041,0,0,325,7095,0,0;QS=3,0;MQSB=0.814433;MQ0F=0 PL:DP:DV 0,30,206:10:0 0,15,104:5:0 0,9,98:3:0 17 1271 . T 0 . DP=18;I16=12,5,0,0,620,22824,0,0,989,58441,0,0,298,6422,0,0;QS=3,0;MQSB=0.818731;MQ0F=0 PL:DP:DV 0,27,240:9:0 0,15,138:5:0 0,9,109:3:0 17 1272 . C 0 . DP=17;I16=11,5,0,0,597,22587,0,0,929,54841,0,0,297,6393,0,0;QS=3,0;MQSB=0.823561;MQ0F=0 PL:DP:DV 0,27,251:9:0 0,12,113:4:0 0,9,110:3:0 17 1273 . A 0 . DP=18;I16=12,6,0,0,633,23063,0,0,1049,62041,0,0,318,6866,0,0;QS=3,0;MQSB=0.85394;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,109:4:0 0,9,113:3:0 17 1274 . T 0 . DP=17;I16=10,6,0,0,609,23335,0,0,929,54841,0,0,293,6205,0,0;QS=3,0;MQSB=0.863243;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,6,67:2:0 0,9,115:3:0 17 1275 . G 0 . DP=17;I16=11,6,0,0,602,21976,0,0,989,58441,0,0,317,6763,0,0;QS=3,0;MQSB=0.85832;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,87:3:0 0,9,112:3:0 17 1276 . T 0 . DP=17;I16=11,6,0,0,587,20925,0,0,989,58441,0,0,316,6714,0,0;QS=3,0;MQSB=0.85832;MQ0F=0 PL:DP:DV 0,33,248:11:0 0,9,94:3:0 0,9,110:3:0 17 1277 . C 0 . DP=18;I16=11,7,0,0,590,20126,0,0,1049,62041,0,0,314,6634,0,0;QS=3,0;MQSB=0.883327;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,85:3:0 0,9,106:3:0 17 1278 . G 0 . DP=18;I16=11,7,0,0,603,20675,0,0,1049,62041,0,0,313,6575,0,0;QS=3,0;MQSB=0.883327;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,77:3:0 0,9,96:3:0 17 1279 . G 0 . DP=18;I16=11,7,0,0,646,23662,0,0,1049,62041,0,0,312,6538,0,0;QS=3,0;MQSB=0.883327;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,85:3:0 0,9,113:3:0 17 1280 . T 0 . DP=18;I16=10,7,0,0,558,19192,0,0,989,58441,0,0,296,6298,0,0;QS=3,0;MQSB=0.887766;MQ0F=0 PL:DP:DV 0,33,249:11:0 0,9,86:3:0 0,9,97:3:0 17 1281 . G 0 . DP=17;I16=10,6,0,0,564,21244,0,0,929,54841,0,0,270,5658,0,0;QS=3,0;MQSB=0.863243;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,85:3:0 0,9,103:3:0 17 1282 . C 0 . DP=18;I16=10,8,0,0,654,24308,0,0,1049,62041,0,0,294,6286,0,0;QS=3,0;MQSB=0.906029;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,94:3:0 0,12,131:4:0 17 1283 . T 0 . DP=18;I16=10,8,0,0,677,26313,0,0,1049,62041,0,0,294,6308,0,0;QS=3,0;MQSB=0.906029;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,83:3:0 0,12,154:4:0 17 1284 . T 0 . DP=18;I16=10,8,0,0,631,22949,0,0,1049,62041,0,0,293,6301,0,0;QS=3,0;MQSB=0.906029;MQ0F=0 PL:DP:DV 0,33,254:11:0 0,9,92:3:0 0,12,145:4:0 17 1285 . G 0 . DP=18;I16=10,7,0,0,657,25545,0,0,989,58441,0,0,267,5691,0,0;QS=3,0;MQSB=0.887766;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,94:3:0 0,12,145:4:0 17 1286 . G 0 . DP=18;I16=10,8,0,0,650,24684,0,0,1049,62041,0,0,291,6353,0,0;QS=3,0;MQSB=0.906029;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,78:3:0 0,12,148:4:0 17 1287 . A 0 . DP=17;I16=9,8,0,0,609,22229,0,0,989,58441,0,0,291,6411,0,0;QS=3,0;MQSB=0.91051;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,91:3:0 0,12,137:4:0 17 1288 . A 0 . DP=17;I16=9,8,0,0,605,22315,0,0,989,58441,0,0,290,6438,0,0;QS=3,0;MQSB=0.91051;MQ0F=0 PL:DP:DV 0,30,245:10:0 0,9,103:3:0 0,12,140:4:0 17 1289 . T 0 . DP=18;I16=10,8,0,0,637,23207,0,0,1049,62041,0,0,289,6483,0,0;QS=3,0;MQSB=0.906029;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,99:3:0 0,12,141:4:0 17 1290 . G 0 . DP=15;I16=9,6,0,0,559,21163,0,0,869,51241,0,0,292,6544,0,0;QS=3,0;MQSB=0.868815;MQ0F=0 PL:DP:DV 0,27,242:9:0 0,9,97:3:0 0,9,106:3:0 17 1291 . T 0 . DP=15;I16=9,6,0,0,547,20303,0,0,869,51241,0,0,295,6619,0,0;QS=3,0;MQSB=0.868815;MQ0F=0 PL:DP:DV 0,27,225:9:0 0,9,91:3:0 0,9,117:3:0 17 1292 . T 0 . DP=15;I16=9,6,0,0,559,21121,0,0,869,51241,0,0,297,6657,0,0;QS=3,0;MQSB=0.868815;MQ0F=0 PL:DP:DV 0,27,236:9:0 0,9,95:3:0 0,9,113:3:0 17 1293 . T 0 . DP=15;I16=9,6,0,0,578,22428,0,0,869,51241,0,0,299,6707,0,0;QS=3,0;MQSB=0.868815;MQ0F=0 PL:DP:DV 0,27,242:9:0 0,9,100:3:0 0,9,116:3:0 17 1294 . G 0 . DP=16;I16=9,7,0,0,606,23572,0,0,929,54841,0,0,301,6769,0,0;QS=3,0;MQSB=0.892753;MQ0F=0 PL:DP:DV 0,27,254:9:0 0,12,110:4:0 0,9,119:3:0 17 1295 . G 0 . DP=16;I16=9,7,0,0,567,21419,0,0,929,54841,0,0,304,6844,0,0;QS=3,0;MQSB=0.892753;MQ0F=0 PL:DP:DV 0,27,244:9:0 0,12,102:4:0 0,9,114:3:0 17 1296 . G 0 . DP=16;I16=8,7,0,0,518,19300,0,0,869,51241,0,0,294,6740,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,27,227:9:0 0,9,89:3:0 0,9,114:3:0 17 1297 . G 0 . DP=18;I16=9,8,0,0,551,19323,0,0,958,55682,0,0,322,7444,0,0;QS=3,0;MQSB=0.998843;MQ0F=0 PL:DP:DV 0,30,219:10:0 0,9,86:3:0 0,12,133:4:0 17 1298 . T 0 . DP=18;I16=10,8,0,0,615,22371,0,0,1018,59282,0,0,336,7638,0,0;QS=3,0;MQSB=0.99606;MQ0F=0 PL:DP:DV 0,30,233:10:0 0,12,105:4:0 0,12,138:4:0 17 1299 . T 0 . DP=18;I16=9,8,0,0,619,23135,0,0,958,55682,0,0,328,7548,0,0;QS=3,0;MQSB=0.998843;MQ0F=0 PL:DP:DV 0,30,242:10:0 0,9,97:3:0 0,12,136:4:0 17 1300 . T 0 . DP=18;I16=10,8,0,0,631,23107,0,0,1018,59282,0,0,338,7638,0,0;QS=3,0;MQSB=0.99606;MQ0F=0 PL:DP:DV 0,30,235:10:0 0,12,121:4:0 0,12,136:4:0 17 1301 . T G, 0 . DP=18;I16=8,8,1,0,599,22623,18,324,929,54841,29,841,314,7040,25,625;QS=2.9434,0.0566038,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.998843;BQB=1;MQ0F=0 PL:DP:DV 0,9,213,24,216,222:9:1 0,12,126,12,126,126:4:0 0,12,135,12,135,135:4:0 17 1302 . G 0 . DP=17;I16=9,8,0,0,624,23544,0,0,958,55682,0,0,341,7709,0,0;QS=3,0;MQSB=0.998843;MQ0F=0 PL:DP:DV 0,27,240:9:0 0,12,127:4:0 0,12,140:4:0 17 1303 . G 0 . DP=17;I16=9,8,0,0,582,21200,0,0,958,55682,0,0,342,7718,0,0;QS=3,0;MQSB=0.998843;MQ0F=0 PL:DP:DV 0,27,217:9:0 0,12,125:4:0 0,12,138:4:0 17 1304 . A 0 . DP=18;I16=10,8,0,0,652,24124,0,0,1018,59282,0,0,343,7741,0,0;QS=3,0;MQSB=0.99606;MQ0F=0 PL:DP:DV 0,27,218:9:0 0,12,131:4:0 0,15,169:5:0 17 1305 . T 0 . DP=18;I16=10,8,0,0,685,26381,0,0,1018,59282,0,0,345,7779,0,0;QS=3,0;MQSB=0.99606;MQ0F=0 PL:DP:DV 0,27,241:9:0 0,12,122:4:0 0,15,174:5:0 17 1306 . T 0 . DP=18;I16=9,8,0,0,644,24628,0,0,958,55682,0,0,345,7829,0,0;QS=3,0;MQSB=0.998843;MQ0F=0 PL:DP:DV 0,27,236:9:0 0,9,106:3:0 0,15,168:5:0 17 1307 . T 0 . DP=17;I16=9,8,0,0,628,23746,0,0,958,55682,0,0,348,7902,0,0;QS=3,0;MQSB=0.998843;MQ0F=0 PL:DP:DV 0,27,226:9:0 0,9,104:3:0 0,15,167:5:0 17 1308 . A 0 . DP=19;I16=9,10,0,0,690,25500,0,0,1078,62882,0,0,350,7938,0,0;QS=3,0;MQSB=0.999167;MQ0F=0 PL:DP:DV 0,30,236:10:0 0,12,127:4:0 0,15,173:5:0 17 1309 . C 0 . DP=19;I16=9,9,0,0,698,27148,0,0,1049,62041,0,0,342,7818,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,106:3:0 0,15,169:5:0 17 1310 . A 0 . DP=19;I16=9,10,0,0,751,29953,0,0,1078,62882,0,0,356,7958,0,0;QS=3,0;MQSB=0.999167;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,132:4:0 0,15,183:5:0 17 1311 . G 0 . DP=19;I16=9,10,0,0,742,29384,0,0,1078,62882,0,0,359,7995,0,0;QS=3,0;MQSB=0.999167;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,128:4:0 0,15,174:5:0 17 1312 . C 0 . DP=19;I16=9,10,0,0,717,27783,0,0,1078,62882,0,0,362,8050,0,0;QS=3,0;MQSB=0.999167;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,115:4:0 0,15,166:5:0 17 1313 . T 0 . DP=19;I16=9,10,0,0,751,30061,0,0,1078,62882,0,0,364,8074,0,0;QS=3,0;MQSB=0.999167;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,135:4:0 0,15,175:5:0 17 1314 . T 0 . DP=19;I16=9,10,0,0,707,26983,0,0,1078,62882,0,0,366,8118,0,0;QS=3,0;MQSB=0.999167;MQ0F=0 PL:DP:DV 0,30,245:10:0 0,12,134:4:0 0,15,174:5:0 17 1315 . T 0 . DP=19;I16=9,10,0,0,717,27491,0,0,1078,62882,0,0,367,8131,0,0;QS=3,0;MQSB=0.999167;MQ0F=0 PL:DP:DV 0,30,250:10:0 0,12,132:4:0 0,15,174:5:0 17 1316 . G 0 . DP=21;I16=10,11,0,0,776,29502,0,0,1198,70082,0,0,368,8162,0,0;QS=3,0;MQSB=0.99938;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,128:4:0 0,18,188:6:0 17 1317 . G 0 . DP=21;I16=10,11,0,0,751,27855,0,0,1198,70082,0,0,371,8213,0,0;QS=3,0;MQSB=0.99938;MQ0F=0 PL:DP:DV 0,33,253:11:0 0,12,130:4:0 0,18,179:6:0 17 1318 . G 0 . DP=21;I16=10,11,0,0,749,27663,0,0,1198,70082,0,0,372,8188,0,0;QS=3,0;MQSB=0.99938;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,130:4:0 0,18,184:6:0 17 1319 . A 0 . DP=21;I16=10,11,0,0,768,28390,0,0,1198,70082,0,0,373,8189,0,0;QS=3,0;MQSB=0.99938;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,124:4:0 0,18,186:6:0 17 1320 . C 0 . DP=21;I16=10,11,0,0,728,25496,0,0,1198,70082,0,0,373,8165,0,0;QS=3,0;MQSB=0.99938;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,113:4:0 0,18,161:6:0 17 1321 . G 0 . DP=22;I16=10,12,0,0,762,27412,0,0,1289,76441,0,0,374,8164,0,0;QS=3,0;MQSB=0.924723;MQ0F=0 PL:DP:DV 0,33,244:11:0 0,9,104:3:0 0,24,208:8:0 17 1322 . C 0 . DP=22;I16=10,12,0,0,852,33528,0,0,1289,76441,0,0,377,8187,0,0;QS=3,0;MQSB=0.924723;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,111:3:0 0,24,225:8:0 17 1323 . T 0 . DP=22;I16=10,12,0,0,874,35152,0,0,1289,76441,0,0,379,8185,0,0;QS=3,0;MQSB=0.924723;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,112:3:0 0,24,237:8:0 17 1324 . C 0 . DP=21;I16=9,12,0,0,793,30585,0,0,1229,72841,0,0,381,8157,0,0;QS=3,0;MQSB=0.913101;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,109:3:0 0,24,218:8:0 17 1325 . A 0 . DP=21;I16=9,12,0,0,782,29430,0,0,1229,72841,0,0,383,8153,0,0;QS=3,0;MQSB=0.913101;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,105:3:0 0,24,218:8:0 17 1326 . A 0 . DP=21;I16=9,12,0,0,791,30479,0,0,1229,72841,0,0,385,8173,0,0;QS=3,0;MQSB=0.913101;MQ0F=0 PL:DP:DV 0,30,249:10:0 0,9,108:3:0 0,24,230:8:0 17 1327 . C 0 . DP=23;I16=11,12,0,0,834,31048,0,0,1349,80041,0,0,387,8217,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,99:3:0 0,27,237:9:0 17 1328 . C 0 . DP=23;I16=11,12,0,0,878,33972,0,0,1349,80041,0,0,391,8287,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,105:3:0 0,27,254:9:0 17 1329 . T 0 . DP=23;I16=11,12,0,0,882,34756,0,0,1349,80041,0,0,395,8385,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,112:3:0 0,27,255:9:0 17 1330 . G 0 . DP=23;I16=11,12,0,0,856,32638,0,0,1349,80041,0,0,398,8460,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,101:3:0 0,27,241:9:0 17 1331 . T 0 . DP=23;I16=11,12,0,0,847,31785,0,0,1349,80041,0,0,400,8512,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,106:3:0 0,27,247:9:0 17 1332 . A 0 . DP=23;I16=11,12,0,0,826,30264,0,0,1349,80041,0,0,402,8592,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,33,252:11:0 0,9,104:3:0 0,27,238:9:0 17 1333 . C 0 . DP=23;I16=11,12,0,0,836,30928,0,0,1349,80041,0,0,404,8700,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,101:3:0 0,27,236:9:0 17 1334 . C 0 . DP=22;I16=11,11,0,0,830,32380,0,0,1289,76441,0,0,405,8733,0,0;QS=3,0;MQSB=0.936864;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,107:3:0 0,27,238:9:0 17 1335 . T 0 . DP=22;I16=11,11,0,0,877,35371,0,0,1289,76441,0,0,406,8788,0,0;QS=3,0;MQSB=0.936864;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,113:3:0 0,27,253:9:0 17 1336 . C 0 . DP=25;I16=12,12,0,0,890,33648,0,0,1378,80882,0,0,398,8784,0,0;QS=3,0;MQSB=0.786628;MQ0F=0 PL:DP:DV 0,33,250:11:0 0,12,131:4:0 0,27,237:9:0 17 1337 . A 0 . DP=26;I16=13,13,0,0,941,34611,0,0,1498,88082,0,0,411,8967,0,0;QS=3,0;MQSB=0.800737;MQ0F=0 PL:DP:DV 0,33,249:11:0 0,15,152:5:0 0,30,247:10:0 17 1338 . A 0 . DP=26;I16=12,14,0,0,982,37358,0,0,1498,88082,0,0,416,9048,0,0;QS=3,0;MQSB=0.771623;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,153:5:0 0,30,255:10:0 17 1339 . T 0 . DP=27;I16=12,15,0,0,989,36855,0,0,1558,91682,0,0,422,9160,0,0;QS=3,0;MQSB=0.765017;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,151:5:0 0,33,255:11:0 17 1340 . A 0 . DP=27;I16=11,15,0,0,937,34141,0,0,1498,88082,0,0,425,9289,0,0;QS=3,0;MQSB=0.738577;MQ0F=0 PL:DP:DV 0,30,250:10:0 0,15,149:5:0 0,33,254:11:0 17 1341 . A 0 . DP=27;I16=12,14,0,0,929,33961,0,0,1498,88082,0,0,410,8810,0,0;QS=3,0;MQSB=0.771623;MQ0F=0 PL:DP:DV 0,30,245:10:0 0,15,141:5:0 0,33,255:11:0 17 1342 . A 0 . DP=27;I16=12,15,0,0,951,34575,0,0,1558,91682,0,0,439,9499,0,0;QS=3,0;MQSB=0.765017;MQ0F=0 PL:DP:DV 0,33,254:11:0 0,15,159:5:0 0,33,249:11:0 17 1343 . C 0 . DP=25;I16=10,15,0,0,902,33364,0,0,1469,87241,0,0,445,9593,0,0;QS=3,0;MQSB=0.9171;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,140:5:0 0,30,233:10:0 17 1344 . C 0 . DP=26;I16=10,16,0,0,985,37915,0,0,1529,90841,0,0,451,9715,0,0;QS=3,0;MQSB=0.914947;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,163:6:0 0,30,238:10:0 17 1345 . T 0 . DP=26;I16=10,16,0,0,1004,39262,0,0,1529,90841,0,0,458,9866,0,0;QS=3,0;MQSB=0.914947;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,182:6:0 0,30,242:10:0 17 1346 . G 0 . DP=26;I16=10,16,0,0,1003,38983,0,0,1529,90841,0,0,465,10047,0,0;QS=3,0;MQSB=0.914947;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,172:6:0 0,30,239:10:0 17 1347 . A 0 . DP=26;I16=10,16,0,0,995,38409,0,0,1529,90841,0,0,470,10156,0,0;QS=3,0;MQSB=0.914947;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,177:6:0 0,30,243:10:0 17 1348 . T 0 . DP=26;I16=10,16,0,0,988,38126,0,0,1529,90841,0,0,474,10242,0,0;QS=3,0;MQSB=0.914947;MQ0F=0 PL:DP:DV 0,30,252:10:0 0,18,185:6:0 0,30,251:10:0 17 1349 . T 0 . DP=26;I16=9,15,0,0,905,34409,0,0,1409,83641,0,0,454,9730,0,0;QS=3,0;MQSB=0.904837;MQ0F=0 PL:DP:DV 0,24,225:8:0 0,18,176:6:0 0,30,248:10:0 17 1350 . T 0 . DP=26;I16=10,16,0,0,975,36909,0,0,1498,88082,0,0,485,10495,0,0;QS=3,0;MQSB=0.700784;MQ0F=0 PL:DP:DV 0,27,242:9:0 0,21,195:7:0 0,30,249:10:0 17 1351 . T 0 . DP=26;I16=10,16,0,0,956,35602,0,0,1498,88082,0,0,491,10663,0,0;QS=3,0;MQSB=0.700784;MQ0F=0 PL:DP:DV 0,27,238:9:0 0,21,193:7:0 0,30,240:10:0 17 1352 . A 0 . DP=26;I16=10,16,0,0,901,31965,0,0,1498,88082,0,0,496,10810,0,0;QS=3,0;MQSB=0.700784;MQ0F=0 PL:DP:DV 0,27,250:9:0 0,21,180:7:0 0,30,211:10:0 17 1353 . A 0 . DP=26;I16=10,16,0,0,927,33583,0,0,1498,88082,0,0,499,10885,0,0;QS=3,0;MQSB=0.700784;MQ0F=0 PL:DP:DV 0,27,240:9:0 0,21,187:7:0 0,30,224:10:0 17 1354 . A 0 . DP=26;I16=10,16,0,0,927,33621,0,0,1498,88082,0,0,502,10986,0,0;QS=3,0;MQSB=0.700784;MQ0F=0 PL:DP:DV 0,27,237:9:0 0,21,190:7:0 0,30,223:10:0 17 1355 . A 0 . DP=26;I16=10,16,0,0,924,33736,0,0,1498,88082,0,0,505,11113,0,0;QS=3,0;MQSB=0.700784;MQ0F=0 PL:DP:DV 0,27,239:9:0 0,21,186:7:0 0,30,227:10:0 17 1356 . A 0 . DP=25;I16=10,15,0,0,916,34050,0,0,1438,84482,0,0,509,11265,0,0;QS=3,0;MQSB=0.707404;MQ0F=0 PL:DP:DV 0,27,249:9:0 0,21,178:7:0 0,27,226:9:0 17 1357 . A 0 . DP=25;I16=9,15,0,0,949,38007,0,0,1378,80882,0,0,488,10816,0,0;QS=3,0;MQSB=0.67032;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,21,193:7:0 0,24,217:8:0 17 1358 . G 0 . DP=25;I16=9,15,0,0,873,32435,0,0,1378,80882,0,0,491,10967,0,0;QS=3,0;MQSB=0.67032;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,21,172:7:0 0,24,188:8:0 17 1359 . T 0 . DP=25;I16=9,15,0,0,857,31139,0,0,1378,80882,0,0,494,11144,0,0;QS=3,0;MQSB=0.67032;MQ0F=0 PL:DP:DV 0,27,232:9:0 0,21,184:7:0 0,24,196:8:0 17 1360 . T 0 . DP=25;I16=9,15,0,0,879,32419,0,0,1378,80882,0,0,497,11347,0,0;QS=3,0;MQSB=0.67032;MQ0F=0 PL:DP:DV 0,27,248:9:0 0,21,184:7:0 0,24,192:8:0 17 1361 . T 0 . DP=27;I16=9,17,0,0,936,34238,0,0,1498,88082,0,0,500,11576,0,0;QS=3,0;MQSB=0.657209;MQ0F=0 PL:DP:DV 0,27,234:9:0 0,24,201:8:0 0,27,206:9:0 17 1362 . G 0 . DP=26;I16=9,17,0,0,973,36785,0,0,1498,88082,0,0,502,11680,0,0;QS=3,0;MQSB=0.657209;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,24,202:8:0 0,27,207:9:0 17 1363 . G 0 . DP=25;I16=8,17,0,0,936,35390,0,0,1438,84482,0,0,504,11756,0,0;QS=3,0;MQSB=0.612391;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,21,181:7:0 0,27,202:9:0 17 1364 . G 0 . DP=25;I16=8,17,0,0,902,32840,0,0,1438,84482,0,0,504,11752,0,0;QS=3,0;MQSB=0.612391;MQ0F=0 PL:DP:DV 0,27,233:9:0 0,21,184:7:0 0,27,200:9:0 17 1365 . G 0 . DP=26;I16=9,17,0,0,946,35224,0,0,1498,88082,0,0,503,11717,0,0;QS=3,0;MQSB=0.657209;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,24,190:8:0 0,27,202:9:0 17 1366 . G 0 . DP=25;I16=8,15,0,0,840,31058,0,0,1318,77282,0,0,454,10450,0,0;QS=3,0;MQSB=0.625784;MQ0F=0 PL:DP:DV 0,21,215:7:0 0,21,181:7:0 0,27,199:9:0 17 1367 . G C, 0 . DP=25;I16=7,16,0,1,836,30888,27,729,1349,80041,60,3600,472,11152,15,225;QS=2.91641,0.0835913,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.864405;BQB=1;MQ0F=0 PL:DP:DV 0,24,236,24,236,236:8:0 0,21,179,21,179,179:7:0 0,0,171,24,174,189:9:1 17 1368 . A 0 . DP=26;I16=8,17,0,0,869,30839,0,0,1438,84482,0,0,481,11095,0,0;QS=3,0;MQSB=0.612391;MQ0F=0 PL:DP:DV 0,27,240:9:0 0,21,180:7:0 0,27,197:9:0 17 1369 . T 0 . DP=26;I16=8,18,0,0,902,32160,0,0,1498,88082,0,0,508,11758,0,0;QS=3,0;MQSB=0.606531;MQ0F=0 PL:DP:DV 0,27,242:9:0 0,24,197:8:0 0,27,194:9:0 17 1370 . T 0 . DP=27;I16=8,17,0,0,926,34736,0,0,1438,84482,0,0,458,10466,0,0;QS=3,0;MQSB=0.612391;MQ0F=0 PL:DP:DV 0,27,238:9:0 0,21,195:7:0 0,27,215:9:0 17 1371 . C 0 . DP=27;I16=8,19,0,0,929,33255,0,0,1558,91682,0,0,509,11695,0,0;QS=3,0;MQSB=0.601139;MQ0F=0 PL:DP:DV 0,27,241:9:0 0,24,196:8:0 0,30,196:10:0 17 1372 . C 0 . DP=27;I16=8,19,0,0,954,34882,0,0,1558,91682,0,0,510,11696,0,0;QS=3,0;MQSB=0.601139;MQ0F=0 PL:DP:DV 0,27,251:9:0 0,24,198:8:0 0,30,200:10:0 17 1373 . C 0 . DP=26;I16=8,17,0,0,892,32692,0,0,1438,84482,0,0,486,11044,0,0;QS=3,0;MQSB=0.612391;MQ0F=0 PL:DP:DV 0,24,243:8:0 0,21,186:7:0 0,30,195:10:0 17 1374 . C 0 . DP=26;I16=8,17,0,0,953,36553,0,0,1438,84482,0,0,487,11039,0,0;QS=3,0;MQSB=0.612391;MQ0F=0 PL:DP:DV 0,24,249:8:0 0,21,193:7:0 0,30,211:10:0 17 1375 . T 0 . DP=27;I16=9,18,0,0,1011,38377,0,0,1558,91682,0,0,512,11630,0,0;QS=3,0;MQSB=0.651439;MQ0F=0 PL:DP:DV 0,27,243:9:0 0,24,215:8:0 0,30,215:10:0 17 1376 . A 0 . DP=27;I16=9,17,0,0,912,32444,0,0,1498,88082,0,0,488,10992,0,0;QS=3,0;MQSB=0.657209;MQ0F=0 PL:DP:DV 0,27,234:9:0 0,21,198:7:0 0,30,195:10:0 17 1377 . A 0 . DP=26;I16=9,17,0,0,994,38518,0,0,1498,88082,0,0,515,11625,0,0;QS=3,0;MQSB=0.657209;MQ0F=0 PL:DP:DV 0,24,228:8:0 0,24,221:8:0 0,30,228:10:0 17 1378 . G 0 . DP=26;I16=9,17,0,0,910,33180,0,0,1498,88082,0,0,517,11653,0,0;QS=3,0;MQSB=0.657209;MQ0F=0 PL:DP:DV 0,24,234:8:0 0,24,199:8:0 0,30,197:10:0 17 1379 . C 0 . DP=26;I16=9,17,0,0,891,31779,0,0,1498,88082,0,0,519,11701,0,0;QS=3,0;MQSB=0.657209;MQ0F=0 PL:DP:DV 0,24,228:8:0 0,24,198:8:0 0,30,193:10:0 17 1380 . C 0 . DP=26;I16=9,17,0,0,925,33925,0,0,1498,88082,0,0,520,11720,0,0;QS=3,0;MQSB=0.657209;MQ0F=0 PL:DP:DV 0,24,226:8:0 0,24,202:8:0 0,30,209:10:0 17 1381 . C 0 . DP=28;I16=10,18,0,0,878,28560,0,0,1618,95282,0,0,521,11761,0,0;QS=3,0;MQSB=0.689069;MQ0F=0 PL:DP:DV 0,24,211:8:0 0,24,177:8:0 0,36,208:12:0 17 1382 . G 0 . DP=28;I16=8,18,0,0,905,32553,0,0,1529,90841,0,0,482,10912,0,0;QS=3,0;MQSB=0.882497;MQ0F=0 PL:DP:DV 0,24,213:8:0 0,18,152:6:0 0,36,243:12:0 17 1383 . C 0 . DP=27;I16=10,16,0,0,897,32373,0,0,1498,88082,0,0,502,11242,0,0;QS=3,0;MQSB=0.700784;MQ0F=0 PL:DP:DV 0,24,225:8:0 0,21,163:7:0 0,33,241:11:0 17 1384 . C 0 . DP=28;I16=11,17,0,0,980,35558,0,0,1618,95282,0,0,529,11885,0,0;QS=3,0;MQSB=0.726331;MQ0F=0 PL:DP:DV 0,27,248:9:0 0,24,184:8:0 0,33,239:11:0 17 1385 . A 0 . DP=30;I16=11,18,0,0,949,33215,0,0,1678,98882,0,0,508,11356,0,0;QS=3,0;MQSB=0.720887;MQ0F=0 PL:DP:DV 0,30,232:10:0 0,27,185:9:0 0,30,234:10:0 17 1386 . C 0 . DP=30;I16=11,19,0,0,1088,39938,0,0,1738,102482,0,0,537,12011,0,0;QS=3,0;MQSB=0.715831;MQ0F=0 PL:DP:DV 0,30,248:10:0 0,27,218:9:0 0,33,255:11:0 17 1387 . C 0 . DP=31;I16=12,19,0,0,1101,40297,0,0,1798,106082,0,0,540,12022,0,0;QS=3,0;MQSB=0.743137;MQ0F=0 PL:DP:DV 0,33,250:11:0 0,27,219:9:0 0,33,252:11:0 17 1388 . C 0 . DP=31;I16=11,19,0,0,999,34081,0,0,1769,105241,0,0,519,11439,0,0;QS=3,0;MQSB=0.91982;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,24,185:8:0 0,33,211:11:0 17 1389 . G 0 . DP=31;I16=11,19,0,0,1065,39119,0,0,1738,102482,0,0,535,11941,0,0;QS=3,0;MQSB=0.715831;MQ0F=0 PL:DP:DV 0,27,234:9:0 0,30,234:10:0 0,33,255:11:0 17 1390 . G 0 . DP=31;I16=12,19,0,0,1152,43744,0,0,1798,106082,0,0,555,12241,0,0;QS=3,0;MQSB=0.743137;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,30,245:10:0 0,33,255:11:0 17 1391 . A 0 . DP=31;I16=12,19,0,0,1241,50255,0,0,1798,106082,0,0,560,12326,0,0;QS=3,0;MQSB=0.743137;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,30,255:10:0 0,33,255:11:0 17 1392 . G 0 . DP=31;I16=12,19,0,0,1160,44364,0,0,1798,106082,0,0,564,12392,0,0;QS=3,0;MQSB=0.743137;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,30,250:10:0 0,33,255:11:0 17 1393 . A 0 . DP=31;I16=12,19,0,0,1123,41811,0,0,1798,106082,0,0,568,12490,0,0;QS=3,0;MQSB=0.743137;MQ0F=0 PL:DP:DV 0,30,228:10:0 0,30,255:10:0 0,33,255:11:0 17 1394 . C 0 . DP=31;I16=12,19,0,0,1139,42555,0,0,1798,106082,0,0,571,12569,0,0;QS=3,0;MQSB=0.743137;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,30,244:10:0 0,33,255:11:0 17 1395 . A 0 . DP=31;I16=12,19,0,0,1206,48048,0,0,1798,106082,0,0,575,12677,0,0;QS=3,0;MQSB=0.743137;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,240:9:0 0,33,255:11:0 17 1396 . G 0 . DP=31;I16=12,19,0,0,1196,46882,0,0,1798,106082,0,0,579,12763,0,0;QS=3,0;MQSB=0.743137;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,249:9:0 0,33,255:11:0 17 1397 . C 0 . DP=31;I16=12,18,0,0,993,33339,0,0,1738,102482,0,0,579,12775,0,0;QS=3,0;MQSB=0.748022;MQ0F=0 PL:DP:DV 0,30,231:10:0 0,27,205:9:0 0,33,217:11:0 17 1398 . G 0 . DP=30;I16=12,18,0,0,1055,38309,0,0,1738,102482,0,0,584,12826,0,0;QS=3,0;MQSB=0.748022;MQ0F=0 PL:DP:DV 0,33,250:11:0 0,27,229:9:0 0,30,244:10:0 17 1399 . G 0 . DP=30;I16=12,18,0,0,1132,43666,0,0,1738,102482,0,0,586,12854,0,0;QS=3,0;MQSB=0.748022;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,239:9:0 0,30,255:10:0 17 1400 . A 0 . DP=30;I16=11,18,0,0,1101,42271,0,0,1678,98882,0,0,563,12289,0,0;QS=3,0;MQSB=0.720887;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,27,239:9:0 0,30,246:10:0 17 1401 . T 0 . DP=30;I16=12,18,0,0,1130,44170,0,0,1738,102482,0,0,589,12955,0,0;QS=3,0;MQSB=0.748022;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,251:9:0 0,30,255:10:0 17 1402 . T 0 . DP=31;I16=13,18,0,0,1152,44058,0,0,1798,106082,0,0,589,12977,0,0;QS=3,0;MQSB=0.771348;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,244:9:0 0,30,255:10:0 17 1403 . T 0 . DP=31;I16=13,18,0,0,1178,45296,0,0,1798,106082,0,0,590,13032,0,0;QS=3,0;MQSB=0.771348;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,249:9:0 0,30,255:10:0 17 1404 . C 0 . DP=31;I16=13,18,0,0,1102,40838,0,0,1798,106082,0,0,591,13121,0,0;QS=3,0;MQSB=0.771348;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,236:9:0 0,30,244:10:0 17 1405 . C 0 . DP=30;I16=12,18,0,0,1154,45216,0,0,1738,102482,0,0,593,13243,0,0;QS=3,0;MQSB=0.748022;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,241:9:0 0,27,229:9:0 17 1406 . T 0 . DP=30;I16=12,18,0,0,1153,44919,0,0,1738,102482,0,0,595,13397,0,0;QS=3,0;MQSB=0.748022;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,248:9:0 0,27,223:9:0 17 1407 . T 0 . DP=30;I16=11,18,0,0,1074,40182,0,0,1678,98882,0,0,570,12856,0,0;QS=3,0;MQSB=0.720887;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,231:9:0 0,27,215:9:0 17 1408 . A 0 . DP=30;I16=12,18,0,0,1131,43363,0,0,1738,102482,0,0,596,13592,0,0;QS=3,0;MQSB=0.748022;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,30,244:10:0 0,27,234:9:0 17 1409 . G 0 . DP=29;I16=12,17,0,0,1082,40752,0,0,1678,98882,0,0,599,13729,0,0;QS=3,0;MQSB=0.753269;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,237:9:0 0,27,204:9:0 17 1410 . T 0 . DP=29;I16=12,17,0,0,1084,41030,0,0,1678,98882,0,0,601,13841,0,0;QS=3,0;MQSB=0.753269;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,239:9:0 0,27,218:9:0 17 1411 . T 0 . DP=29;I16=12,17,0,0,958,32550,0,0,1678,98882,0,0,600,13826,0,0;QS=3,0;MQSB=0.753269;MQ0F=0 PL:DP:DV 0,33,250:11:0 0,27,206:9:0 0,27,194:9:0 17 1412 . A T, 0 . DP=29;I16=11,17,1,0,924,31586,25,625,1649,98041,29,841,573,13159,24,576;QS=2.90842,0.0915751,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.753269;BQB=1;MQ0F=0 PL:DP:DV 0,33,245,33,245,245:11:0 0,2,171,24,174,187:9:1 0,27,192,27,192,192:9:0 17 1413 . C 0 . DP=29;I16=12,17,0,0,1067,39941,0,0,1678,98882,0,0,591,13521,0,0;QS=3,0;MQSB=0.753269;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,230:9:0 0,27,209:9:0 17 1414 . T 0 . DP=29;I16=12,17,0,0,1123,44271,0,0,1678,98882,0,0,585,13335,0,0;QS=3,0;MQSB=0.753269;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,27,236:9:0 0,27,221:9:0 17 1415 . T 0 . DP=29;I16=12,17,0,0,1000,35254,0,0,1678,98882,0,0,577,13077,0,0;QS=3,0;MQSB=0.753269;MQ0F=0 PL:DP:DV 0,33,246:11:0 0,27,216:9:0 0,27,208:9:0 17 1416 . A 0 . DP=30;I16=13,17,0,0,1026,36124,0,0,1738,102482,0,0,569,12847,0,0;QS=3,0;MQSB=0.776389;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,208:9:0 0,27,199:9:0 17 1417 . C 0 . DP=29;I16=13,16,0,0,1111,42941,0,0,1678,98882,0,0,563,12645,0,0;QS=3,0;MQSB=0.781802;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,235:9:0 0,24,211:8:0 17 1418 . T 0 . DP=29;I16=13,16,0,0,1111,43021,0,0,1678,98882,0,0,557,12471,0,0;QS=3,0;MQSB=0.781802;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,231:9:0 0,24,207:8:0 17 1419 . A 0 . DP=29;I16=13,16,0,0,1039,38191,0,0,1678,98882,0,0,551,12325,0,0;QS=3,0;MQSB=0.781802;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,222:9:0 0,24,199:8:0 17 1420 . T 0 . DP=29;I16=13,16,0,0,1039,37885,0,0,1678,98882,0,0,544,12158,0,0;QS=3,0;MQSB=0.781802;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,27,234:9:0 0,24,185:8:0 17 1421 . G 0 . DP=31;I16=13,18,0,0,1129,41649,0,0,1798,106082,0,0,536,11970,0,0;QS=3,0;MQSB=0.771348;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,30,247:10:0 0,24,191:8:0 17 1422 . C 0 . DP=29;I16=13,16,0,0,1075,40645,0,0,1678,98882,0,0,532,11810,0,0;QS=3,0;MQSB=0.781802;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,30,239:10:0 0,18,180:6:0 17 1423 . T 0 . DP=31;I16=14,17,0,0,1179,45767,0,0,1798,106082,0,0,528,11678,0,0;QS=3,0;MQSB=0.79638;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,36,255:12:0 0,18,183:6:0 17 1424 . C 0 . DP=30;I16=13,17,0,0,1086,40448,0,0,1738,102482,0,0,527,11575,0,0;QS=3,0;MQSB=0.776389;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,36,255:12:0 0,18,174:6:0 17 1425 . C 0 . DP=30;I16=13,17,0,0,1124,42974,0,0,1738,102482,0,0,525,11453,0,0;QS=3,0;MQSB=0.776389;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,36,255:12:0 0,18,180:6:0 17 1426 . T 0 . DP=32;I16=15,17,0,0,1218,47130,0,0,1858,109682,0,0,523,11363,0,0;QS=3,0;MQSB=0.813784;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,36,255:12:0 0,18,188:6:0 17 1427 . T 0 . DP=32;I16=14,18,0,0,1126,40772,0,0,1827,106923,0,0,524,11306,0,0;QS=3,0;MQSB=0.916401;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,36,255:12:0 0,18,176:6:0 17 1428 . G 0 . DP=33;I16=15,18,0,0,1188,43596,0,0,1887,110523,0,0,525,11233,0,0;QS=3,0;MQSB=0.930476;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,36,255:12:0 0,18,166:6:0 17 1429 . G 0 . DP=33;I16=14,18,0,0,1070,37720,0,0,1858,109682,0,0,507,10795,0,0;QS=3,0;MQSB=0.997118;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,33,243:11:0 0,18,148:6:0 17 1430 . C 0 . DP=33;I16=15,18,0,0,1145,40795,0,0,1887,110523,0,0,529,11193,0,0;QS=3,0;MQSB=0.930476;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,36,247:12:0 0,18,157:6:0 17 1431 . C 0 . DP=33;I16=15,18,0,0,1160,41686,0,0,1887,110523,0,0,531,11227,0,0;QS=3,0;MQSB=0.930476;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,36,253:12:0 0,18,167:6:0 17 1432 . A 0 . DP=33;I16=15,18,0,0,1118,39032,0,0,1887,110523,0,0,533,11297,0,0;QS=3,0;MQSB=0.930476;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,36,255:12:0 0,18,169:6:0 17 1433 . T 0 . DP=34;I16=15,19,0,0,1238,45602,0,0,1947,114123,0,0,535,11403,0,0;QS=3,0;MQSB=0.923533;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,36,255:12:0 0,18,174:6:0 17 1434 . T 0 . DP=35;I16=15,20,0,0,1269,46757,0,0,2007,117723,0,0,537,11495,0,0;QS=3,0;MQSB=0.916855;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,36,255:12:0 0,18,179:6:0 17 1435 . T 0 . DP=35;I16=14,20,0,0,1256,46868,0,0,1947,114123,0,0,515,10999,0,0;QS=3,0;MQSB=0.901704;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,36,255:12:0 0,18,180:6:0 17 1436 . C 0 . DP=34;I16=14,20,0,0,1250,46978,0,0,1947,114123,0,0,544,11790,0,0;QS=3,0;MQSB=0.901704;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,36,255:12:0 0,18,165:6:0 17 1437 . T 0 . DP=32;I16=13,19,0,0,1222,47206,0,0,1858,109682,0,0,548,11892,0,0;QS=3,0;MQSB=0.993397;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,33,255:11:0 0,15,161:5:0 17 1438 . C 0 . DP=30;I16=13,17,0,0,1132,43284,0,0,1738,102482,0,0,554,12028,0,0;QS=3,0;MQSB=0.996503;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,30,251:10:0 0,15,159:5:0 17 1439 . T 0 . DP=30;I16=13,17,0,0,1110,41602,0,0,1738,102482,0,0,560,12196,0,0;QS=3,0;MQSB=0.996503;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,30,255:10:0 0,15,156:5:0 17 1440 . A 0 . DP=30;I16=12,16,0,0,1062,41028,0,0,1618,95282,0,0,550,12106,0,0;QS=3,0;MQSB=0.995699;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,27,255:9:0 0,12,144:4:0 17 1441 . G 0 . DP=30;I16=13,17,0,0,1138,44104,0,0,1738,102482,0,0,574,12576,0,0;QS=3,0;MQSB=0.996503;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,30,244:10:0 0,12,137:4:0 17 1442 . G 0 . DP=30;I16=13,17,0,0,1064,38534,0,0,1738,102482,0,0,580,12740,0,0;QS=3,0;MQSB=0.996503;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,30,242:10:0 0,12,128:4:0 17 1443 . T 0 . DP=30;I16=12,17,0,0,1013,36225,0,0,1678,98882,0,0,568,12598,0,0;QS=3,0;MQSB=0.993891;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,30,230:10:0 0,12,133:4:0 17 1444 . A 0 . DP=30;I16=12,17,0,0,968,33936,0,0,1678,98882,0,0,569,12627,0,0;QS=3,0;MQSB=0.993891;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,27,205:9:0 0,12,132:4:0 17 1445 . T 0 . DP=30;I16=14,15,0,0,1053,39129,0,0,1678,98882,0,0,585,13161,0,0;QS=3,0;MQSB=0.999762;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,27,237:9:0 0,15,169:5:0 17 1446 . T 0 . DP=30;I16=13,16,0,0,1051,38675,0,0,1678,98882,0,0,579,12951,0,0;QS=3,0;MQSB=0.997839;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,24,229:8:0 0,15,164:5:0 17 1447 . G 0 . DP=30;I16=14,16,0,0,1110,42692,0,0,1738,102482,0,0,606,13612,0,0;QS=3,0;MQSB=0.999136;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,27,229:9:0 0,15,157:5:0 17 1448 . G 0 . DP=30;I16=13,16,0,0,1037,37759,0,0,1678,98882,0,0,585,13151,0,0;QS=3,0;MQSB=0.997839;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,24,217:8:0 0,15,150:5:0 17 1449 . T 0 . DP=30;I16=14,16,0,0,1051,37869,0,0,1738,102482,0,0,612,13870,0,0;QS=3,0;MQSB=0.999136;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,27,230:9:0 0,15,152:5:0 17 1450 . A 0 . DP=29;I16=13,15,0,0,1039,38785,0,0,1649,98041,0,0,604,13842,0,0;QS=3,0;MQSB=0.956162;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,24,224:8:0 0,15,165:5:0 17 1451 . T 0 . DP=29;I16=13,16,0,0,1046,38586,0,0,1709,101641,0,0,616,14042,0,0;QS=3,0;MQSB=0.960189;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,24,228:8:0 0,15,158:5:0 17 1452 . A 0 . DP=31;I16=14,16,0,0,1066,38696,0,0,1769,105241,0,0,604,13924,0,0;QS=3,0;MQSB=0.958545;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,218:8:0 0,15,147:5:0 17 1453 . T 0 . DP=31;I16=13,17,0,0,1107,42059,0,0,1769,105241,0,0,592,13444,0,0;QS=3,0;MQSB=0.963674;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,199:7:0 0,15,165:5:0 17 1454 . T 0 . DP=31;I16=14,17,0,0,1139,42711,0,0,1829,108841,0,0,617,14045,0,0;QS=3,0;MQSB=0.962133;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,24,234:8:0 0,15,166:5:0 17 1455 . G 0 . DP=31;I16=13,17,0,0,1107,41765,0,0,1769,105241,0,0,592,13420,0,0;QS=3,0;MQSB=0.963674;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,197:7:0 0,15,148:5:0 17 1456 . T 0 . DP=31;I16=14,17,0,0,1139,42717,0,0,1829,108841,0,0,617,14069,0,0;QS=3,0;MQSB=0.962133;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,24,215:8:0 0,15,170:5:0 17 1457 . G 0 . DP=31;I16=14,17,0,0,1129,42071,0,0,1829,108841,0,0,615,14019,0,0;QS=3,0;MQSB=0.962133;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,24,212:8:0 0,15,164:5:0 17 1458 . T 0 . DP=31;I16=14,17,0,0,1101,40243,0,0,1829,108841,0,0,613,13997,0,0;QS=3,0;MQSB=0.962133;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,24,220:8:0 0,15,165:5:0 17 1459 . C A, 0 . DP=31;I16=14,16,0,1,1164,45842,24,576,1769,105241,60,3600,608,13948,2,4;QS=2.87234,0.12766,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.962133;BQB=1;MQ0F=0 PL:DP:DV 0,54,255,54,255,255:18:0 0,24,224,24,224,224:8:0 9,0,135,21,138,152:5:1 17 1460 . T 0 . DP=32;I16=15,17,0,0,1243,48813,0,0,1889,112441,0,0,605,13833,0,0;QS=3,0;MQSB=0.960687;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,24,240:8:0 0,15,170:5:0 17 1461 . G 0 . DP=32;I16=15,17,0,0,1171,44033,0,0,1889,112441,0,0,600,13692,0,0;QS=3,0;MQSB=0.960687;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,24,224:8:0 0,15,157:5:0 17 1462 . C 0 . DP=30;I16=14,15,0,0,1102,42216,0,0,1709,101641,0,0,579,13241,0,0;QS=3,0;MQSB=0.954405;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,202:7:0 0,9,112:3:0 17 1463 . T 0 . DP=30;I16=15,15,0,0,1128,43348,0,0,1769,105241,0,0,592,13396,0,0;QS=3,0;MQSB=0.952765;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,209:7:0 0,12,139:4:0 17 1464 . G 0 . DP=31;I16=14,16,0,0,1095,40557,0,0,1769,105241,0,0,563,12665,0,0;QS=3,0;MQSB=0.958545;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,18,186:6:0 0,15,144:5:0 17 1465 . T 0 . DP=31;I16=15,16,0,0,1143,42557,0,0,1829,108841,0,0,584,13164,0,0;QS=3,0;MQSB=0.957006;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,204:7:0 0,15,166:5:0 17 1466 . G 0 . DP=31;I16=15,16,0,0,1122,42294,0,0,1829,108841,0,0,580,13018,0,0;QS=3,0;MQSB=0.957006;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,206:7:0 0,15,163:5:0 17 1467 . A 0 . DP=31;I16=14,16,0,0,1031,36341,0,0,1769,105241,0,0,569,12803,0,0;QS=3,0;MQSB=0.958545;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,195:7:0 0,15,155:5:0 17 1468 . A 0 . DP=30;I16=15,15,0,0,991,34477,0,0,1769,105241,0,0,573,12717,0,0;QS=3,0;MQSB=0.952765;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,177:7:0 0,15,158:5:0 17 1469 . C T, 0 . DP=31;I16=13,15,1,0,1013,37887,38,1444,1649,98041,60,3600,536,12106,9,81;QS=2.94025,0.0597484,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.954405;BQB=1;MQ0F=0 PL:DP:DV 0,16,255,51,255,255:18:1 0,18,178,18,178,178:6:0 0,15,160,15,160,160:5:0 17 1470 . T 0 . DP=31;I16=16,15,0,0,1110,41070,0,0,1829,108841,0,0,567,12489,0,0;QS=3,0;MQSB=0.951229;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,210:7:0 0,15,165:5:0 17 1471 . G 0 . DP=30;I16=16,14,0,0,1051,38425,0,0,1769,105241,0,0,564,12348,0,0;QS=3,0;MQSB=0.946202;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,192:7:0 0,12,128:4:0 17 1472 . T 0 . DP=30;I16=16,14,0,0,1049,38097,0,0,1769,105241,0,0,561,12237,0,0;QS=3,0;MQSB=0.946202;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,200:7:0 0,12,134:4:0 17 1473 . C 0 . DP=30;I16=16,14,0,0,1058,38526,0,0,1769,105241,0,0,558,12156,0,0;QS=3,0;MQSB=0.946202;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,175:7:0 0,12,140:4:0 17 1474 . C 0 . DP=30;I16=16,14,0,0,1120,42756,0,0,1769,105241,0,0,555,12105,0,0;QS=3,0;MQSB=0.946202;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,202:7:0 0,12,141:4:0 17 1475 . T 0 . DP=29;I16=14,14,0,0,1047,40395,0,0,1649,98041,0,0,537,11827,0,0;QS=3,0;MQSB=0.949591;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,208:7:0 0,12,155:4:0 17 1476 . T G, 0 . DP=31;I16=15,15,1,0,1056,37884,14,196,1769,105241,60,3600,566,12614,10,100;QS=2.944,0.056,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.951229;BQB=1;MQ0F=0 PL:DP:DV 0,57,255,57,255,255:19:0 0,9,177,21,180,183:8:1 0,12,140,12,140,140:4:0 17 1477 . G 0 . DP=31;I16=16,15,0,0,1150,43390,0,0,1829,108841,0,0,574,12700,0,0;QS=3,0;MQSB=0.951229;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,24,217:8:0 0,12,138:4:0 17 1478 . G 0 . DP=31;I16=14,15,0,0,1008,36638,0,0,1709,101641,0,0,542,11982,0,0;QS=3,0;MQSB=0.954405;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,24,208:8:0 0,9,109:3:0 17 1479 . C 0 . DP=30;I16=15,15,0,0,1061,38671,0,0,1769,105241,0,0,564,12556,0,0;QS=3,0;MQSB=0.952765;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,24,211:8:0 0,9,106:3:0 17 1480 . C 0 . DP=30;I16=15,15,0,0,1083,40355,0,0,1769,105241,0,0,561,12531,0,0;QS=3,0;MQSB=0.952765;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,24,220:8:0 0,9,103:3:0 17 1481 . T 0 . DP=30;I16=15,15,0,0,1102,41308,0,0,1769,105241,0,0,558,12532,0,0;QS=3,0;MQSB=0.952765;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,24,235:8:0 0,9,105:3:0 17 1482 . G 0 . DP=29;I16=15,14,0,0,1044,38550,0,0,1709,101641,0,0,556,12558,0,0;QS=3,0;MQSB=0.947838;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,24,210:8:0 0,6,75:2:0 17 1483 . T 0 . DP=31;I16=14,16,0,0,1042,37190,0,0,1769,105241,0,0,521,11919,0,0;QS=3,0;MQSB=0.958545;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,21,215:7:0 0,9,100:3:0 17 1484 . T 0 . DP=31;I16=15,16,0,0,1067,37837,0,0,1829,108841,0,0,547,12587,0,0;QS=3,0;MQSB=0.957006;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,24,216:8:0 0,9,106:3:0 17 1485 . T 0 . DP=30;I16=15,15,0,0,1027,35863,0,0,1769,105241,0,0,549,12659,0,0;QS=3,0;MQSB=0.952765;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,24,209:8:0 0,9,93:3:0 17 1486 . G 0 . DP=29;I16=15,14,0,0,1089,41679,0,0,1709,101641,0,0,551,12707,0,0;QS=3,0;MQSB=0.947838;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,198:7:0 0,9,108:3:0 17 1487 . G 0 . DP=28;I16=14,14,0,0,973,34723,0,0,1649,98041,0,0,554,12778,0,0;QS=3,0;MQSB=0.949591;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,196:7:0 0,9,97:3:0 17 1488 . T 0 . DP=28;I16=13,14,0,0,928,32432,0,0,1589,94441,0,0,532,12246,0,0;QS=3,0;MQSB=0.951472;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,176:6:0 0,9,102:3:0 17 1489 . G 0 . DP=27;I16=13,14,0,0,958,35292,0,0,1589,94441,0,0,535,12361,0,0;QS=3,0;MQSB=0.951472;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,174:6:0 0,9,93:3:0 17 1490 . A 0 . DP=27;I16=13,14,0,0,928,33108,0,0,1589,94441,0,0,538,12446,0,0;QS=3,0;MQSB=0.951472;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,160:5:0 0,9,94:3:0 17 1491 . C 0 . DP=27;I16=12,13,0,0,798,26812,0,0,1469,87241,0,0,499,11587,0,0;QS=3,0;MQSB=0.948139;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,12,108:4:0 0,9,79:3:0 17 1492 . G 0 . DP=27;I16=13,14,0,0,893,30927,0,0,1589,94441,0,0,543,12527,0,0;QS=3,0;MQSB=0.951472;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,136:5:0 0,9,100:3:0 17 1493 . G 0 . DP=27;I16=13,13,0,0,907,32761,0,0,1529,90841,0,0,520,11948,0,0;QS=3,0;MQSB=0.945959;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,15,155:5:0 0,9,106:3:0 17 1494 . G 0 . DP=27;I16=13,14,0,0,939,33599,0,0,1589,94441,0,0,547,12639,0,0;QS=3,0;MQSB=0.951472;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,156:5:0 0,9,107:3:0 17 1495 . T 0 . DP=26;I16=12,13,0,0,814,27700,0,0,1469,87241,0,0,524,12048,0,0;QS=3,0;MQSB=0.948139;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,15,143:5:0 0,9,105:3:0 17 1496 . G 0 . DP=26;I16=13,13,0,0,969,36751,0,0,1529,90841,0,0,550,12674,0,0;QS=3,0;MQSB=0.945959;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,15,157:5:0 0,9,107:3:0 17 1497 . A 0 . DP=27;I16=12,14,0,0,904,32740,0,0,1529,90841,0,0,525,12019,0,0;QS=3,0;MQSB=0.953497;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,15,166:5:0 0,9,113:3:0 17 1498 . G 0 . DP=27;I16=13,14,0,0,1002,37852,0,0,1589,94441,0,0,551,12635,0,0;QS=3,0;MQSB=0.951472;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,153:5:0 0,9,112:3:0 17 1499 . G 0 . DP=28;I16=14,14,0,0,973,34891,0,0,1649,98041,0,0,551,12599,0,0;QS=3,0;MQSB=0.949591;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,15,146:5:0 0,9,108:3:0 17 1500 . A 0 . DP=28;I16=14,14,0,0,1023,38115,0,0,1649,98041,0,0,552,12588,0,0;QS=3,0;MQSB=0.949591;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,15,165:5:0 0,9,115:3:0 17 1501 . G 0 . DP=28;I16=14,14,0,0,1051,40105,0,0,1649,98041,0,0,551,12505,0,0;QS=3,0;MQSB=0.949591;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,15,163:5:0 0,9,100:3:0 17 1502 . C 0 . DP=27;I16=13,14,0,0,963,35241,0,0,1589,94441,0,0,549,12351,0,0;QS=3,0;MQSB=0.951472;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,140:5:0 0,9,105:3:0 17 1503 . A 0 . DP=28;I16=14,14,0,0,1026,38252,0,0,1649,98041,0,0,546,12176,0,0;QS=3,0;MQSB=0.949591;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,15,171:5:0 0,9,109:3:0 17 1504 . G 0 . DP=28;I16=13,14,0,0,1052,41226,0,0,1589,94441,0,0,523,11591,0,0;QS=3,0;MQSB=0.951472;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,163:5:0 0,9,111:3:0 17 1505 . G 0 . DP=28;I16=14,13,0,0,959,35545,0,0,1589,94441,0,0,517,11295,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,155:5:0 0,9,114:3:0 17 1506 . G 0 . DP=28;I16=14,14,0,0,1013,37355,0,0,1649,98041,0,0,540,11840,0,0;QS=3,0;MQSB=0.949591;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,15,139:5:0 0,9,112:3:0 17 1507 . A 0 . DP=28;I16=14,14,0,0,987,35217,0,0,1649,98041,0,0,538,11792,0,0;QS=3,0;MQSB=0.949591;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,15,162:5:0 0,9,114:3:0 17 1508 . C 0 . DP=28;I16=14,14,0,0,1033,38663,0,0,1649,98041,0,0,535,11727,0,0;QS=3,0;MQSB=0.949591;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,15,137:5:0 0,9,112:3:0 17 1509 . A 0 . DP=28;I16=15,13,0,0,1028,38610,0,0,1649,98041,0,0,529,11493,0,0;QS=3,0;MQSB=0.942064;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,12,128:4:0 0,9,122:3:0 17 1510 . G 0 . DP=28;I16=15,13,0,0,1064,40824,0,0,1649,98041,0,0,524,11288,0,0;QS=3,0;MQSB=0.942064;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,12,132:4:0 0,9,111:3:0 17 1511 . A 0 . DP=28;I16=15,13,0,0,976,34794,0,0,1649,98041,0,0,519,11113,0,0;QS=3,0;MQSB=0.942064;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,12,136:4:0 0,9,110:3:0 17 1512 . A 0 . DP=28;I16=15,12,0,0,998,37460,0,0,1589,94441,0,0,489,10343,0,0;QS=3,0;MQSB=0.935229;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,12,142:4:0 0,9,110:3:0 17 1513 . G 0 . DP=28;I16=14,13,0,0,1014,38940,0,0,1589,94441,0,0,497,10709,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,12,134:4:0 0,9,108:3:0 17 1514 . G 0 . DP=28;I16=15,13,0,0,1054,39954,0,0,1649,98041,0,0,504,10768,0,0;QS=3,0;MQSB=0.942064;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,12,134:4:0 0,9,108:3:0 17 1515 . G 0 . DP=28;I16=14,13,0,0,948,34152,0,0,1589,94441,0,0,488,10564,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,12,133:4:0 0,9,101:3:0 17 1516 . T 0 . DP=27;I16=12,13,0,0,811,27385,0,0,1469,87241,0,0,472,10338,0,0;QS=3,0;MQSB=0.948139;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,12,117:4:0 0,9,106:3:0 17 1517 . C 0 . DP=27;I16=13,13,0,0,943,35153,0,0,1529,90841,0,0,478,10380,0,0;QS=3,0;MQSB=0.945959;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,12,134:4:0 0,9,97:3:0 17 1518 . C 0 . DP=28;I16=13,12,0,0,908,33852,0,0,1469,87241,0,0,427,9261,0,0;QS=3,0;MQSB=0.939413;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,12,132:4:0 0,9,96:3:0 17 1519 . T 0 . DP=28;I16=15,13,0,0,988,35808,0,0,1649,98041,0,0,475,10337,0,0;QS=3,0;MQSB=0.942064;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,15,154:5:0 0,9,110:3:0 17 1520 . G 0 . DP=29;I16=16,13,0,0,1005,36307,0,0,1709,101641,0,0,470,10328,0,0;QS=3,0;MQSB=0.940317;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,18,150:6:0 0,9,104:3:0 17 1521 . C 0 . DP=28;I16=16,12,0,0,900,30324,0,0,1649,98041,0,0,466,10300,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,18,164:6:0 0,9,88:3:0 17 1522 . G 0 . DP=27;I16=15,10,0,0,784,25144,0,0,1469,87241,0,0,442,9956,0,0;QS=3,0;MQSB=0.9171;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,15,110:5:0 0,9,91:3:0 17 1523 . T 0 . DP=27;I16=16,11,0,0,921,32001,0,0,1589,94441,0,0,457,10189,0,0;QS=3,0;MQSB=0.925036;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,136:5:0 0,9,108:3:0 17 1524 . G 0 . DP=27;I16=16,10,0,0,932,34140,0,0,1529,90841,0,0,453,10153,0,0;QS=3,0;MQSB=0.914947;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,12,108:4:0 0,9,103:3:0 17 1525 . C 0 . DP=27;I16=16,11,0,0,972,35704,0,0,1589,94441,0,0,473,10719,0,0;QS=3,0;MQSB=0.925036;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,12,113:4:0 0,9,112:3:0 17 1526 . C 0 . DP=25;I16=14,11,0,0,883,31843,0,0,1469,87241,0,0,470,10684,0,0;QS=3,0;MQSB=0.929204;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,12,109:4:0 0,9,106:3:0 17 1527 . C 0 . DP=24;I16=14,10,0,0,867,31999,0,0,1440,86400,0,0,466,10572,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,107:4:0 0,9,112:3:0 17 1528 . T 0 . DP=23;I16=13,10,0,0,869,33133,0,0,1380,82800,0,0,463,10483,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,114:4:0 0,9,113:3:0 17 1529 . G 0 . DP=23;I16=13,10,0,0,812,29432,0,0,1380,82800,0,0,459,10365,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,108:4:0 0,9,104:3:0 17 1530 . C 0 . DP=24;I16=13,10,0,0,819,30073,0,0,1380,82800,0,0,446,10186,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,137:5:0 0,9,106:3:0 17 1531 . C 0 . DP=24;I16=13,11,0,0,877,32969,0,0,1440,86400,0,0,452,10190,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,144:5:0 0,9,105:3:0 17 1532 . T 0 . DP=24;I16=13,11,0,0,887,33721,0,0,1440,86400,0,0,449,10135,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,165:5:0 0,9,120:3:0 17 1533 . T 0 . DP=23;I16=13,10,0,0,793,27987,0,0,1380,82800,0,0,447,10101,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,146:5:0 0,9,107:3:0 17 1534 . C 0 . DP=23;I16=14,9,0,0,847,31627,0,0,1380,82800,0,0,446,10086,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,153:5:0 0,9,109:3:0 17 1535 . A 0 . DP=23;I16=14,9,0,0,770,26604,0,0,1380,82800,0,0,444,9990,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,144:5:0 0,9,99:3:0 17 1536 . C 0 . DP=24;I16=15,9,0,0,826,28984,0,0,1440,86400,0,0,442,9914,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,138:5:0 0,9,110:3:0 17 1537 . A 0 . DP=24;I16=14,9,0,0,844,31384,0,0,1380,82800,0,0,416,9234,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,149:5:0 0,9,115:3:0 17 1538 . A 0 . DP=24;I16=15,9,0,0,909,34727,0,0,1440,86400,0,0,440,9826,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,154:5:0 0,9,112:3:0 17 1539 . G 0 . DP=24;I16=15,9,0,0,913,35327,0,0,1440,86400,0,0,439,9815,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,149:5:0 0,9,117:3:0 17 1540 . C 0 . DP=23;I16=15,8,0,0,828,30260,0,0,1380,82800,0,0,438,9776,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,146:5:0 0,9,102:3:0 17 1541 . C 0 . DP=23;I16=15,8,0,0,823,30615,0,0,1380,82800,0,0,437,9759,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,142:5:0 0,9,108:3:0 17 1542 . C 0 . DP=25;I16=16,8,0,0,893,33843,0,0,1440,86400,0,0,435,9715,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,147:5:0 0,12,131:4:0 17 1543 . C 0 . DP=25;I16=16,8,0,0,880,33206,0,0,1440,86400,0,0,434,9696,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,141:5:0 0,12,136:4:0 17 1544 . T 0 . DP=25;I16=16,8,0,0,899,34405,0,0,1440,86400,0,0,431,9603,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,150:5:0 0,12,148:4:0 17 1545 . G 0 . DP=25;I16=16,8,0,0,874,32636,0,0,1440,86400,0,0,428,9536,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,146:5:0 0,12,129:4:0 17 1546 . G 0 . DP=24;I16=15,8,0,0,796,28796,0,0,1380,82800,0,0,425,9443,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,130:5:0 0,9,110:3:0 17 1547 . A 0 . DP=23;I16=15,8,0,0,837,30945,0,0,1380,82800,0,0,448,9996,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,176:6:0 0,9,112:3:0 17 1548 . A 0 . DP=23;I16=14,8,0,0,812,30830,0,0,1320,79200,0,0,421,9319,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,173:5:0 0,9,115:3:0 17 1549 . G 0 . DP=23;I16=15,8,0,0,870,33642,0,0,1380,82800,0,0,444,9912,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,174:6:0 0,9,114:3:0 17 1550 . G 0 . DP=23;I16=15,8,0,0,790,28502,0,0,1380,82800,0,0,442,9900,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,154:6:0 0,9,109:3:0 17 1551 . A 0 . DP=23;I16=15,8,0,0,802,28596,0,0,1380,82800,0,0,440,9908,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,173:6:0 0,9,107:3:0 17 1552 . A 0 . DP=21;I16=14,7,0,0,792,30156,0,0,1260,75600,0,0,438,9836,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,196:6:0 0,9,110:3:0 17 1553 . A 0 . DP=21;I16=13,7,0,0,738,28112,0,0,1200,72000,0,0,411,9159,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,251:11:0 0,18,192:6:0 0,9,115:3:0 17 1554 . G 0 . DP=21;I16=14,7,0,0,763,28221,0,0,1260,75600,0,0,434,9752,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,171:6:0 0,9,101:3:0 17 1555 . T 0 . DP=21;I16=14,7,0,0,726,26234,0,0,1260,75600,0,0,432,9740,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,252:12:0 0,18,169:6:0 0,9,108:3:0 17 1556 . T 0 . DP=21;I16=14,7,0,0,745,26971,0,0,1260,75600,0,0,429,9697,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,253:12:0 0,18,177:6:0 0,9,110:3:0 17 1557 . G 0 . DP=22;I16=15,7,0,0,801,29689,0,0,1320,79200,0,0,426,9672,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,166:6:0 0,9,101:3:0 17 1558 . T 0 . DP=22;I16=15,6,0,0,720,25620,0,0,1260,75600,0,0,404,9244,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,131:5:0 0,9,112:3:0 17 1559 . T 0 . DP=22;I16=15,7,0,0,712,24690,0,0,1320,79200,0,0,417,9439,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,248:13:0 0,18,158:6:0 0,9,103:3:0 17 1560 . T 0 . DP=21;I16=14,7,0,0,736,26560,0,0,1260,75600,0,0,412,9284,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,251:12:0 0,18,170:6:0 0,9,112:3:0 17 1561 . T 0 . DP=21;I16=14,7,0,0,706,24776,0,0,1260,75600,0,0,406,9102,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,241:12:0 0,18,174:6:0 0,9,106:3:0 17 1562 . G 0 . DP=21;I16=14,7,0,0,765,28655,0,0,1260,75600,0,0,399,8893,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,181:6:0 0,9,108:3:0 17 1563 . G 0 . DP=22;I16=13,7,0,0,713,26255,0,0,1200,72000,0,0,360,8176,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,147:5:0 0,9,108:3:0 17 1564 . G 0 . DP=22;I16=13,8,0,0,721,25457,0,0,1260,75600,0,0,368,8218,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,173:6:0 0,9,95:3:0 17 1565 . A 0 . DP=21;I16=14,7,0,0,724,25888,0,0,1260,75600,0,0,380,8352,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,162:6:0 0,6,71:2:0 17 1566 . T 0 . DP=21;I16=13,7,0,0,698,25286,0,0,1200,72000,0,0,364,8086,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,143:5:0 0,6,78:2:0 17 1567 . C 0 . DP=20;I16=12,6,0,0,652,24422,0,0,1080,64800,0,0,351,7881,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,104:3:0 0,6,72:2:0 17 1568 . T 0 . DP=20;I16=13,7,0,0,711,26125,0,0,1200,72000,0,0,363,7871,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,157:5:0 0,6,74:2:0 17 1569 . C 0 . DP=19;I16=12,6,0,0,676,25850,0,0,1080,64800,0,0,351,7669,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,128:4:0 0,6,76:2:0 17 1570 . T 0 . DP=19;I16=12,7,0,0,700,26750,0,0,1140,68400,0,0,353,7583,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,149:5:0 0,6,71:2:0 17 1571 . G 0 . DP=19;I16=12,6,0,0,639,23361,0,0,1080,64800,0,0,343,7441,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,125:4:0 0,6,70:2:0 17 1572 . C 0 . DP=19;I16=11,6,0,0,640,24568,0,0,1020,61200,0,0,328,7202,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,125:4:0 0,6,70:2:0 17 1573 . A 0 . DP=19;I16=11,4,0,0,565,21475,0,0,900,54000,0,0,304,6900,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,132:4:0 0,3,40:1:0 17 1574 . C 0 . DP=19;I16=12,5,0,0,636,24076,0,0,1020,61200,0,0,318,6948,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,128:4:0 0,3,38:1:0 17 1575 . C 0 . DP=19;I16=11,6,0,0,610,22742,0,0,1020,61200,0,0,296,6272,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,125:4:0 0,6,71:2:0 17 1576 . C 0 . DP=19;I16=12,6,0,0,675,25821,0,0,1080,64800,0,0,315,6785,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,133:4:0 0,6,78:2:0 17 1577 . T 0 . DP=17;I16=11,6,0,0,650,25330,0,0,1020,61200,0,0,310,6692,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,118:3:0 0,6,77:2:0 17 1578 . C 0 . DP=17;I16=10,6,0,0,622,24364,0,0,960,57600,0,0,279,5943,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,84:2:0 0,6,77:2:0 17 1579 . A 0 . DP=17;I16=11,6,0,0,596,22326,0,0,1020,61200,0,0,298,6464,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,116:3:0 0,6,80:2:0 17 1580 . G 0 . DP=17;I16=11,6,0,0,651,25195,0,0,1020,61200,0,0,292,6380,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,109:3:0 0,6,77:2:0 17 1581 . C 0 . DP=17;I16=11,6,0,0,586,21418,0,0,1020,61200,0,0,286,6316,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,103:3:0 0,6,71:2:0 17 1582 . C 0 . DP=18;I16=11,7,0,0,639,23491,0,0,1080,64800,0,0,280,6272,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,109:3:0 0,6,75:2:0 17 1583 . T 0 . DP=16;I16=10,6,0,0,591,22349,0,0,960,57600,0,0,276,6196,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,112:3:0 0,6,74:2:0 17 1584 . G 0 . DP=15;I16=10,5,0,0,563,21283,0,0,900,54000,0,0,272,6086,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,105:3:0 0,3,39:1:0 17 1585 . G 0 . DP=16;I16=10,5,0,0,494,17160,0,0,900,54000,0,0,251,5703,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,244:11:0 0,9,103:3:0 0,3,30:1:0 17 1586 . A 0 . DP=15;I16=10,3,0,0,470,17200,0,0,780,46800,0,0,237,5273,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,223:10:0 0,6,79:2:0 0,3,42:1:0 17 1587 . C 0 . DP=15;I16=11,4,0,0,508,17900,0,0,900,54000,0,0,264,5852,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,247:11:0 0,9,94:3:0 0,3,31:1:0 17 1588 . A 0 . DP=15;I16=11,4,0,0,511,17939,0,0,900,54000,0,0,262,5806,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,232:11:0 0,9,105:3:0 0,3,41:1:0 17 1589 . A 0 . DP=15;I16=11,4,0,0,536,19584,0,0,900,54000,0,0,259,5725,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,249:11:0 0,9,106:3:0 0,3,42:1:0 17 1590 . C 0 . DP=15;I16=10,5,0,0,514,18228,0,0,900,54000,0,0,257,5657,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,231:10:0 0,9,104:3:0 0,6,71:2:0 17 1591 . T 0 . DP=15;I16=10,5,0,0,515,18559,0,0,900,54000,0,0,256,5602,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,241:10:0 0,9,85:3:0 0,6,73:2:0 17 1592 . T 0 . DP=15;I16=10,5,0,0,503,17345,0,0,900,54000,0,0,255,5561,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,225:10:0 0,9,107:3:0 0,6,62:2:0 17 1593 . G 0 . DP=15;I16=10,5,0,0,540,19930,0,0,900,54000,0,0,254,5534,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,250:10:0 0,9,106:3:0 0,6,60:2:0 17 1594 . T 0 . DP=15;I16=10,5,0,0,535,19445,0,0,900,54000,0,0,252,5472,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,234:10:0 0,9,106:3:0 0,6,80:2:0 17 1595 . G 0 . DP=15;I16=10,4,0,0,494,17940,0,0,840,50400,0,0,225,4801,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,213:9:0 0,9,105:3:0 0,6,69:2:0 17 1596 . C 0 . DP=15;I16=10,4,0,0,479,17011,0,0,840,50400,0,0,233,5151,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,200:9:0 0,9,111:3:0 0,6,74:2:0 17 1597 . C 0 . DP=15;I16=10,5,0,0,489,16941,0,0,900,54000,0,0,245,5285,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,195:9:0 0,12,127:4:0 0,6,73:2:0 17 1598 . C 0 . DP=15;I16=10,5,0,0,514,18282,0,0,900,54000,0,0,244,5240,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,207:9:0 0,12,133:4:0 0,6,68:2:0 17 1599 . A 0 . DP=14;I16=8,5,0,0,436,15330,0,0,780,46800,0,0,225,4851,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,182:8:0 0,9,103:3:0 0,6,76:2:0 17 1600 . T 0 . DP=13;I16=8,5,0,0,456,16430,0,0,780,46800,0,0,226,4876,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,193:8:0 0,9,105:3:0 0,6,79:2:0 17 1601 . C 0 . DP=13;I16=8,4,0,0,431,15861,0,0,720,43200,0,0,208,4554,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,171:7:0 0,9,108:3:0 0,6,78:2:0 17 1602 . T 0 . DP=13;I16=7,5,0,0,439,16867,0,0,720,43200,0,0,228,4968,0,0;QS=3,0;MQSB=0.95494;MQ0F=0 PL:DP:DV 0,21,179:7:0 0,9,115:3:0 0,6,85:2:0 17 1603 . G 0 . DP=12;I16=7,5,0,0,455,17407,0,0,720,43200,0,0,230,5034,0,0;QS=3,0;MQSB=0.95494;MQ0F=0 PL:DP:DV 0,21,202:7:0 0,9,111:3:0 0,6,75:2:0 17 1604 . G 0 . DP=12;I16=7,5,0,0,362,11620,0,0,720,43200,0,0,232,5112,0,0;QS=3,0;MQSB=0.95494;MQ0F=0 PL:DP:DV 0,21,163:7:0 0,9,84:3:0 0,6,66:2:0 17 1605 . T 0 . DP=12;I16=7,5,0,0,410,14230,0,0,720,43200,0,0,234,5202,0,0;QS=3,0;MQSB=0.95494;MQ0F=0 PL:DP:DV 0,21,190:7:0 0,9,97:3:0 0,6,61:2:0 17 1606 . G 0 . DP=12;I16=7,4,0,0,370,12738,0,0,660,39600,0,0,211,4679,0,0;QS=3,0;MQSB=0.964642;MQ0F=0 PL:DP:DV 0,18,165:6:0 0,9,91:3:0 0,6,56:2:0 17 1607 . A 0 . DP=12;I16=7,4,0,0,337,11369,0,0,660,39600,0,0,226,5222,0,0;QS=3,0;MQSB=0.964642;MQ0F=0 PL:DP:DV 0,21,161:7:0 0,6,70:2:0 0,6,55:2:0 17 1608 . C 0 . DP=12;I16=7,4,0,0,366,12898,0,0,660,39600,0,0,211,4727,0,0;QS=3,0;MQSB=0.964642;MQ0F=0 PL:DP:DV 0,18,141:6:0 0,9,111:3:0 0,6,62:2:0 17 1609 . C 0 . DP=11;I16=6,5,0,0,365,12889,0,0,660,39600,0,0,237,5393,0,0;QS=3,0;MQSB=0.950952;MQ0F=0 PL:DP:DV 0,18,149:6:0 0,9,110:3:0 0,6,71:2:0 17 1610 . C 0 . DP=11;I16=5,5,0,0,313,10713,0,0,600,36000,0,0,213,4819,0,0;QS=3,0;MQSB=0.952347;MQ0F=0 PL:DP:DV 0,15,127:5:0 0,9,106:3:0 0,6,57:2:0 17 1611 . C 0 . DP=11;I16=6,5,0,0,398,14904,0,0,660,39600,0,0,238,5454,0,0;QS=3,0;MQSB=0.950952;MQ0F=0 PL:DP:DV 0,18,180:6:0 0,9,110:3:0 0,6,67:2:0 17 1612 . T 0 . DP=11;I16=6,5,0,0,409,15421,0,0,660,39600,0,0,238,5472,0,0;QS=3,0;MQSB=0.950952;MQ0F=0 PL:DP:DV 0,18,174:6:0 0,9,107:3:0 0,6,83:2:0 17 1613 . C 0 . DP=11;I16=6,5,0,0,372,12904,0,0,660,39600,0,0,238,5498,0,0;QS=3,0;MQSB=0.950952;MQ0F=0 PL:DP:DV 0,18,169:6:0 0,9,99:3:0 0,6,63:2:0 17 1614 . A 0 . DP=11;I16=6,5,0,0,353,12139,0,0,660,39600,0,0,238,5532,0,0;QS=3,0;MQSB=0.950952;MQ0F=0 PL:DP:DV 0,18,147:6:0 0,9,98:3:0 0,6,69:2:0 17 1615 . C 0 . DP=11;I16=6,5,0,0,366,13080,0,0,660,39600,0,0,238,5574,0,0;QS=3,0;MQSB=0.950952;MQ0F=0 PL:DP:DV 0,18,171:6:0 0,9,105:3:0 0,6,51:2:0 17 1616 . T 0 . DP=11;I16=6,3,0,0,348,13606,0,0,540,32400,0,0,187,4323,0,0;QS=3,0;MQSB=0.924584;MQ0F=0 PL:DP:DV 0,15,159:5:0 0,9,105:3:0 0,3,42:1:0 17 1617 . C 0 . DP=12;I16=6,3,0,0,330,12316,0,0,540,32400,0,0,185,4279,0,0;QS=3,0;MQSB=0.924584;MQ0F=0 PL:DP:DV 0,15,146:5:0 0,9,105:3:0 0,3,42:1:0 17 1618 . A 0 . DP=12;I16=5,6,0,0,385,14199,0,0,629,36841,0,0,244,5636,0,0;QS=3,0;MQSB=0.891517;MQ0F=0 PL:DP:DV 0,18,151:6:0 0,9,93:3:0 0,6,81:2:0 17 1619 . G 0 . DP=11;I16=5,6,0,0,377,13119,0,0,629,36841,0,0,242,5544,0,0;QS=3,0;MQSB=0.891517;MQ0F=0 PL:DP:DV 0,18,166:6:0 0,9,84:3:0 0,6,67:2:0 17 1620 . C 0 . DP=11;I16=5,5,0,0,323,10965,0,0,569,33241,0,0,215,4839,0,0;QS=3,0;MQSB=0.857112;MQ0F=0 PL:DP:DV 0,15,142:5:0 0,9,78:3:0 0,6,56:2:0 17 1621 . C 0 . DP=12;I16=5,7,0,0,363,11779,0,0,689,40441,0,0,263,6021,0,0;QS=3,0;MQSB=0.896474;MQ0F=0 PL:DP:DV 0,21,174:7:0 0,9,80:3:0 0,6,56:2:0 17 1622 . A 0 . DP=12;I16=5,7,0,0,365,12105,0,0,689,40441,0,0,261,5965,0,0;QS=3,0;MQSB=0.896474;MQ0F=0 PL:DP:DV 0,21,176:7:0 0,9,81:3:0 0,6,52:2:0 17 1623 . C 0 . DP=12;I16=5,5,0,0,325,10979,0,0,569,33241,0,0,208,4620,0,0;QS=3,0;MQSB=0.857112;MQ0F=0 PL:DP:DV 0,18,155:6:0 0,9,84:3:0 0,3,37:1:0 17 1624 . C 0 . DP=12;I16=4,6,0,0,336,11718,0,0,569,33241,0,0,211,4799,0,0;QS=3,0;MQSB=0.895781;MQ0F=0 PL:DP:DV 0,18,162:6:0 0,9,88:3:0 0,3,39:1:0 17 1625 . A 0 . DP=12;I16=4,7,0,0,375,13503,0,0,629,36841,0,0,234,5386,0,0;QS=3,0;MQSB=0.924449;MQ0F=0 PL:DP:DV 0,18,158:6:0 0,9,93:3:0 0,6,80:2:0 17 1626 . G 0 . DP=12;I16=5,7,0,0,358,11490,0,0,689,40441,0,0,249,5645,0,0;QS=3,0;MQSB=0.896474;MQ0F=0 PL:DP:DV 0,21,158:7:0 0,9,83:3:0 0,6,63:2:0 17 1627 . A 0 . DP=12;I16=5,7,0,0,363,11609,0,0,689,40441,0,0,246,5590,0,0;QS=3,0;MQSB=0.896474;MQ0F=0 PL:DP:DV 0,21,165:7:0 0,9,75:3:0 0,6,72:2:0 17 1628 . C 0 . DP=12;I16=5,7,0,0,357,11583,0,0,689,40441,0,0,243,5545,0,0;QS=3,0;MQSB=0.896474;MQ0F=0 PL:DP:DV 0,21,165:7:0 0,9,80:3:0 0,6,57:2:0 17 1629 . T 0 . DP=13;I16=6,7,0,0,451,16079,0,0,749,44041,0,0,240,5510,0,0;QS=3,0;MQSB=0.899815;MQ0F=0 PL:DP:DV 0,21,187:7:0 0,12,120:4:0 0,6,79:2:0 17 1630 . T 0 . DP=13;I16=6,7,0,0,403,13323,0,0,749,44041,0,0,237,5435,0,0;QS=3,0;MQSB=0.899815;MQ0F=0 PL:DP:DV 0,21,169:7:0 0,12,111:4:0 0,6,73:2:0 17 1631 . C 0 . DP=12;I16=6,5,0,0,354,12046,0,0,660,39600,0,0,210,4744,0,0;QS=3,0;MQSB=0.950952;MQ0F=0 PL:DP:DV 0,21,184:7:0 0,6,67:2:0 0,6,61:2:0 17 1632 . C 0 . DP=13;I16=6,7,0,0,387,12175,0,0,749,44041,0,0,232,5262,0,0;QS=3,0;MQSB=0.899815;MQ0F=0 PL:DP:DV 0,24,188:8:0 0,9,82:3:0 0,6,61:2:0 17 1633 . A 0 . DP=13;I16=6,7,0,0,404,13272,0,0,749,44041,0,0,230,5166,0,0;QS=3,0;MQSB=0.899815;MQ0F=0 PL:DP:DV 0,24,183:8:0 0,9,86:3:0 0,6,73:2:0 17 1634 . C 0 . DP=13;I16=6,6,0,0,328,9716,0,0,689,40441,0,0,203,4457,0,0;QS=3,0;MQSB=0.864013;MQ0F=0 PL:DP:DV 0,21,151:7:0 0,9,80:3:0 0,6,59:2:0 17 1635 . G 0 . DP=14;I16=6,8,0,0,419,12831,0,0,809,47641,0,0,226,5010,0,0;QS=3,0;MQSB=0.927743;MQ0F=0 PL:DP:DV 0,24,180:8:0 0,12,108:4:0 0,6,59:2:0 17 1636 . A 0 . DP=14;I16=6,8,0,0,418,13400,0,0,809,47641,0,0,225,4951,0,0;QS=3,0;MQSB=0.927743;MQ0F=0 PL:DP:DV 0,24,179:8:0 0,12,110:4:0 0,6,66:2:0 17 1637 . C 0 . DP=14;I16=6,8,0,0,422,13498,0,0,809,47641,0,0,224,4906,0,0;QS=3,0;MQSB=0.927743;MQ0F=0 PL:DP:DV 0,24,185:8:0 0,12,110:4:0 0,6,60:2:0 17 1638 . A 0 . DP=17;I16=9,7,0,0,513,17167,0,0,929,54841,0,0,216,4790,0,0;QS=3,0;MQSB=0.892753;MQ0F=0 PL:DP:DV 0,24,191:8:0 0,18,149:6:0 0,6,78:2:0 17 1639 . G 0 . DP=17;I16=9,8,0,0,552,18844,0,0,989,58441,0,0,223,4765,0,0;QS=3,0;MQSB=0.91051;MQ0F=0 PL:DP:DV 0,27,228:9:0 0,18,150:6:0 0,6,68:2:0 17 1640 . G 0 . DP=17;I16=8,6,0,0,414,13296,0,0,809,47641,0,0,171,3467,0,0;QS=3,0;MQSB=0.875173;MQ0F=0 PL:DP:DV 0,18,150:6:0 0,18,151:6:0 0,6,51:2:0 17 1641 . C 0 . DP=18;I16=9,8,0,0,511,16289,0,0,989,58441,0,0,225,4709,0,0;QS=3,0;MQSB=0.91051;MQ0F=0 PL:DP:DV 0,27,188:9:0 0,18,167:6:0 0,6,65:2:0 17 1642 . T 0 . DP=17;I16=8,7,0,0,519,18513,0,0,869,51241,0,0,217,4613,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,18,170:6:0 0,18,168:6:0 0,9,102:3:0 17 1643 . C 0 . DP=16;I16=7,9,0,0,508,16910,0,0,929,54841,0,0,255,5361,0,0;QS=3,0;MQSB=0.933674;MQ0F=0 PL:DP:DV 0,24,193:8:0 0,18,169:6:0 0,6,56:2:0 17 1644 . C 0 . DP=15;I16=6,9,0,0,514,18056,0,0,869,51241,0,0,259,5401,0,0;QS=3,0;MQSB=0.939413;MQ0F=0 PL:DP:DV 0,21,209:7:0 0,18,165:6:0 0,6,54:2:0 17 1645 . A 0 . DP=15;I16=6,9,0,0,520,18852,0,0,869,51241,0,0,263,5457,0,0;QS=3,0;MQSB=0.939413;MQ0F=0 PL:DP:DV 0,21,213:7:0 0,18,173:6:0 0,6,49:2:0 17 1646 . G 0 . DP=15;I16=6,7,0,0,440,15526,0,0,780,46800,0,0,217,4279,0,0;QS=3,0;MQSB=0.961166;MQ0F=0 PL:DP:DV 0,18,185:6:0 0,15,148:5:0 0,6,48:2:0 17 1647 . C 0 . DP=15;I16=6,9,0,0,433,13883,0,0,869,51241,0,0,271,5617,0,0;QS=3,0;MQSB=0.939413;MQ0F=0 PL:DP:DV 0,21,190:7:0 0,18,139:6:0 0,6,35:2:0 17 1648 . C 0 . DP=15;I16=6,9,0,0,495,16965,0,0,869,51241,0,0,275,5721,0,0;QS=3,0;MQSB=0.939413;MQ0F=0 PL:DP:DV 0,21,203:7:0 0,18,159:6:0 0,6,44:2:0 17 1649 . T 0 . DP=15;I16=6,9,0,0,516,18122,0,0,869,51241,0,0,279,5841,0,0;QS=3,0;MQSB=0.939413;MQ0F=0 PL:DP:DV 0,21,187:7:0 0,18,182:6:0 0,6,56:2:0 17 1650 . C 0 . DP=15;I16=6,7,0,0,405,13551,0,0,749,44041,0,0,240,5028,0,0;QS=3,0;MQSB=0.899815;MQ0F=0 PL:DP:DV 0,18,161:6:0 0,18,169:6:0 0,3,13:1:0 17 1651 . G 0 . DP=17;I16=5,9,0,0,424,13328,0,0,778,44882,0,0,249,5335,0,0;QS=3,0;MQSB=0.965069;MQ0F=0 PL:DP:DV 0,18,150:6:0 0,15,128:5:0 0,9,86:3:0 17 1652 . G 0 . DP=18;I16=7,9,0,0,532,18602,0,0,898,52082,0,0,267,5673,0,0;QS=3,0;MQSB=0.994413;MQ0F=0 PL:DP:DV 0,24,212:8:0 0,18,168:6:0 0,6,54:2:0 17 1653 . C 0 . DP=18;I16=8,10,0,0,581,19565,0,0,1018,59282,0,0,300,6490,0,0;QS=3,0;MQSB=0.99606;MQ0F=0 PL:DP:DV 0,24,208:8:0 0,21,175:7:0 0,9,82:3:0 17 1654 . A T, 0 . DP=18;I16=8,9,0,1,516,16718,15,225,958,55682,60,3600,285,6219,22,484;QS=2.93213,0.0678733,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.99606;BQB=1;MQ0F=0 PL:DP:DV 0,9,155,21,158,162:8:1 0,21,179,21,179,179:7:0 0,9,78,9,78,78:3:0 17 1655 . C 0 . DP=18;I16=8,10,0,0,565,19017,0,0,1018,59282,0,0,313,6887,0,0;QS=3,0;MQSB=0.99606;MQ0F=0 PL:DP:DV 0,24,198:8:0 0,21,180:7:0 0,9,79:3:0 17 1656 . C 0 . DP=19;I16=9,9,0,0,557,18013,0,0,1018,59282,0,0,295,6515,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,202:8:0 0,21,165:7:0 0,9,80:3:0 17 1657 . T A, 0 . DP=18;I16=8,9,0,1,580,20362,15,225,989,58441,29,841,301,6641,25,625;QS=2.93243,0.0675676,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.99606;BQB=1;MQ0F=0 PL:DP:DV 0,24,206,24,206,206:8:0 0,6,158,18,161,165:7:1 0,9,85,9,85,85:3:0 17 1658 . T 0 . DP=19;I16=8,10,0,0,573,19127,0,0,1018,59282,0,0,332,7412,0,0;QS=3,0;MQSB=0.99606;MQ0F=0 PL:DP:DV 0,24,187:8:0 0,21,189:7:0 0,9,91:3:0 17 1659 . C 0 . DP=20;I16=8,10,0,0,609,21517,0,0,1049,62041,0,0,338,7578,0,0;QS=3,0;MQSB=0.906029;MQ0F=0 PL:DP:DV 0,24,219:8:0 0,21,189:7:0 0,9,80:3:0 17 1660 . A 0 . DP=21;I16=8,11,0,0,641,23219,0,0,1078,62882,0,0,385,8901,0,0;QS=3,0;MQSB=0.992359;MQ0F=0 PL:DP:DV 0,21,172:7:0 0,24,219:8:0 0,12,127:4:0 17 1661 . G 0 . DP=21;I16=7,13,0,0,639,21739,0,0,1107,63723,0,0,412,9598,0,0;QS=3,0;MQSB=0.999215;MQ0F=0 PL:DP:DV 0,24,209:8:0 0,24,193:8:0 0,12,98:4:0 17 1662 . C 0 . DP=21;I16=8,12,0,0,641,21423,0,0,1107,63723,0,0,407,9465,0,0;QS=3,0;MQSB=0.988166;MQ0F=0 PL:DP:DV 0,21,192:7:0 0,27,211:9:0 0,12,100:4:0 17 1663 . C 0 . DP=20;I16=7,11,0,0,577,19507,0,0,1018,59282,0,0,394,9204,0,0;QS=3,0;MQSB=0.983729;MQ0F=0 PL:DP:DV 0,21,178:7:0 0,21,192:7:0 0,12,101:4:0 17 1664 . A C, 0 . DP=20;I16=6,10,0,1,530,17982,13,169,898,52082,29,841,358,8422,25,625;QS=2.93953,0.0604651,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.998738;BQB=1;MQ0F=0 PL:DP:DV 0,18,167,18,167,167:6:0 0,7,154,18,157,159:7:1 0,12,109,12,109,109:4:0 17 1665 . T C, 0 . DP=20;I16=7,11,1,1,592,20170,58,1924,1018,59282,89,4441,396,9188,39,821;QS=2.5913,0.408696,0;VDB=0.1;SGB=0.346553;RPB=0.222222;MQB=0.611111;MQSB=0.988166;BQB=0.944444;MQ0F=0 PL:DP:DV 0,21,185,21,185,185:7:0 0,27,222,27,222,222:9:0 35,0,51,41,57,90:4:2 17 1666 . G 0 . DP=20;I16=8,12,0,0,614,19760,0,0,1107,63723,0,0,435,9947,0,0;QS=3,0;MQSB=0.988166;MQ0F=0 PL:DP:DV 0,21,173:7:0 0,27,206:9:0 0,12,111:4:0 17 1667 . G 0 . DP=20;I16=8,11,0,0,617,21033,0,0,1078,62882,0,0,410,9276,0,0;QS=3,0;MQSB=0.992359;MQ0F=0 PL:DP:DV 0,21,183:7:0 0,24,195:8:0 0,12,115:4:0 17 1668 . A 0 . DP=20;I16=7,10,0,0,570,19908,0,0,958,55682,0,0,369,8365,0,0;QS=3,0;MQSB=0.989343;MQ0F=0 PL:DP:DV 0,18,158:6:0 0,21,186:7:0 0,12,124:4:0 17 1669 . C 0 . DP=20;I16=8,12,0,0,640,21336,0,0,1107,63723,0,0,435,9857,0,0;QS=3,0;MQSB=0.988166;MQ0F=0 PL:DP:DV 0,21,172:7:0 0,27,216:9:0 0,12,115:4:0 17 1670 . A 0 . DP=23;I16=9,13,0,0,765,27363,0,0,1258,73682,0,0,410,9234,0,0;QS=3,0;MQSB=0.991121;MQ0F=0 PL:DP:DV 0,27,215:9:0 0,27,233:9:0 0,12,129:4:0 17 1671 . G 0 . DP=23;I16=9,13,0,0,703,23631,0,0,1258,73682,0,0,412,9206,0,0;QS=3,0;MQSB=0.991121;MQ0F=0 PL:DP:DV 0,27,214:9:0 0,27,210:9:0 0,12,106:4:0 17 1672 . T 0 . DP=23;I16=9,13,0,0,724,24700,0,0,1258,73682,0,0,414,9202,0,0;QS=3,0;MQSB=0.991121;MQ0F=0 PL:DP:DV 0,27,203:9:0 0,27,232:9:0 0,12,111:4:0 17 1673 . T 0 . DP=25;I16=8,13,0,0,719,25183,0,0,1198,70082,0,0,372,8248,0,0;QS=3,0;MQSB=0.983744;MQ0F=0 PL:DP:DV 0,27,221:9:0 0,24,212:8:0 0,12,112:4:0 17 1674 . C 0 . DP=25;I16=8,14,0,0,755,26561,0,0,1289,76441,0,0,389,8417,0,0;QS=3,0;MQSB=0.892142;MQ0F=0 PL:DP:DV 0,33,244:11:0 0,21,206:7:0 0,12,96:4:0 17 1675 . C 0 . DP=25;I16=9,15,0,0,705,22355,0,0,1347,78123,0,0,447,9897,0,0;QS=3,0;MQSB=0.996008;MQ0F=0 PL:DP:DV 0,33,222:11:0 0,30,212:10:0 0,9,65:3:0 17 1676 . G 0 . DP=25;I16=6,15,0,0,660,21708,0,0,1167,67323,0,0,383,8265,0,0;QS=3,0;MQSB=0.993205;MQ0F=0 PL:DP:DV 0,33,222:11:0 0,21,170:7:0 0,9,96:3:0 17 1677 . C 0 . DP=25;I16=9,13,0,0,681,22869,0,0,1289,76441,0,0,394,8510,0,0;QS=3,0;MQSB=0.910098;MQ0F=0 PL:DP:DV 0,33,225:11:0 0,24,198:8:0 0,9,74:3:0 17 1678 . C 0 . DP=25;I16=9,15,0,0,775,26785,0,0,1347,78123,0,0,438,9496,0,0;QS=3,0;MQSB=0.996008;MQ0F=0 PL:DP:DV 0,33,235:11:0 0,30,231:10:0 0,9,83:3:0 17 1679 . A 0 . DP=25;I16=8,15,0,0,762,27552,0,0,1287,74523,0,0,412,8858,0,0;QS=3,0;MQSB=0.999479;MQ0F=0 PL:DP:DV 0,33,235:11:0 0,27,219:9:0 0,9,106:3:0 17 1680 . G 0 . DP=26;I16=9,17,0,0,843,29213,0,0,1467,85323,0,0,459,10021,0,0;QS=3,0;MQSB=0.999637;MQ0F=0 PL:DP:DV 0,33,253:11:0 0,33,232:11:0 0,12,108:4:0 17 1681 . C 0 . DP=26;I16=8,15,0,0,681,21293,0,0,1318,77282,0,0,407,8999,0,0;QS=3,0;MQSB=0.974802;MQ0F=0 PL:DP:DV 0,33,223:11:0 0,27,191:9:0 0,9,69:3:0 17 1682 . G 0 . DP=25;I16=9,16,0,0,728,22560,0,0,1407,81723,0,0,455,9877,0,0;QS=3,0;MQSB=0.998399;MQ0F=0 PL:DP:DV 0,30,222:10:0 0,33,208:11:0 0,12,98:4:0 17 1683 . T 0 . DP=25;I16=8,15,0,0,807,29159,0,0,1287,74523,0,0,403,8567,0,0;QS=3,0;MQSB=0.999479;MQ0F=0 PL:DP:DV 0,30,250:10:0 0,30,235:10:0 0,9,91:3:0 17 1684 . T 0 . DP=25;I16=8,15,0,0,802,28774,0,0,1318,77282,0,0,438,9612,0,0;QS=3,0;MQSB=0.974802;MQ0F=0 PL:DP:DV 0,27,227:9:0 0,30,237:10:0 0,12,107:4:0 17 1685 . G 0 . DP=24;I16=8,14,0,0,759,27319,0,0,1258,73682,0,0,413,8999,0,0;QS=3,0;MQSB=0.979255;MQ0F=0 PL:DP:DV 0,24,216:8:0 0,30,242:10:0 0,12,110:4:0 17 1686 . C 0 . DP=24;I16=8,15,0,0,793,28073,0,0,1318,77282,0,0,438,9656,0,0;QS=3,0;MQSB=0.974802;MQ0F=0 PL:DP:DV 0,27,225:9:0 0,30,241:10:0 0,12,103:4:0 17 1687 . C 0 . DP=24;I16=7,14,0,0,741,26765,0,0,1198,70082,0,0,388,8458,0,0;QS=3,0;MQSB=0.966484;MQ0F=0 PL:DP:DV 0,24,221:8:0 0,27,229:9:0 0,12,106:4:0 17 1688 . C 0 . DP=24;I16=8,15,0,0,794,28736,0,0,1318,77282,0,0,431,9605,0,0;QS=3,0;MQSB=0.974802;MQ0F=0 PL:DP:DV 0,27,239:9:0 0,30,221:10:0 0,12,120:4:0 17 1689 . T A, 0 . DP=24;I16=8,15,0,1,805,29443,33,1089,1287,74523,60,3600,421,9311,25,625;QS=2.74419,0.255814,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0 PL:DP:DV 0,27,224,27,224,224:9:0 0,33,255,33,255,255:11:0 21,0,79,30,82,106:4:1 17 1690 . C 0 . DP=24;I16=8,16,0,0,864,32420,0,0,1347,78123,0,0,445,10033,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,242:9:0 0,33,255:11:0 0,12,95:4:0 17 1691 . T 0 . DP=22;I16=7,14,0,0,734,27150,0,0,1167,67323,0,0,421,9525,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,225:9:0 0,30,242:10:0 0,6,57:2:0 17 1692 . G 0 . DP=22;I16=7,13,0,0,678,24110,0,0,1107,63723,0,0,400,9176,0,0;QS=3,0;MQSB=0.999215;MQ0F=0 PL:DP:DV 0,24,218:8:0 0,30,218:10:0 0,6,61:2:0 17 1693 . T 0 . DP=24;I16=10,13,0,0,795,28363,0,0,1318,77282,0,0,444,10422,0,0;QS=3,0;MQSB=0.995682;MQ0F=0 PL:DP:DV 0,27,227:9:0 0,30,235:10:0 0,12,104:4:0 17 1694 . T 0 . DP=24;I16=10,13,0,0,771,26775,0,0,1318,77282,0,0,448,10602,0,0;QS=3,0;MQSB=0.995682;MQ0F=0 PL:DP:DV 0,27,221:9:0 0,30,232:10:0 0,12,114:4:0 17 1695 . C 0 . DP=24;I16=10,14,0,0,811,28517,0,0,1347,78123,0,0,454,10806,0,0;QS=3,0;MQSB=0.98469;MQ0F=0 PL:DP:DV 0,27,227:9:0 0,33,239:11:0 0,12,121:4:0 17 1696 . T 0 . DP=23;I16=10,13,0,0,825,30819,0,0,1287,74523,0,0,455,10869,0,0;QS=3,0;MQSB=0.976718;MQ0F=0 PL:DP:DV 0,24,219:8:0 0,33,255:11:0 0,12,108:4:0 17 1697 . G 0 . DP=23;I16=10,13,0,0,767,26757,0,0,1287,74523,0,0,455,10897,0,0;QS=3,0;MQSB=0.976718;MQ0F=0 PL:DP:DV 0,24,208:8:0 0,33,246:11:0 0,12,112:4:0 17 1698 . C A, 0 . DP=21;I16=10,10,0,1,726,27088,27,729,1169,69241,29,841,451,10903,6,36;QS=2.91148,0.0885246,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.99938;BQB=1;MQ0F=0 PL:DP:DV 0,24,229,24,229,229:8:0 0,0,190,24,193,207:9:1 0,12,111,12,111,111:4:0 17 1699 . T 0 . DP=21;I16=9,10,0,0,698,25826,0,0,1078,62882,0,0,408,9692,0,0;QS=3,0;MQSB=0.999167;MQ0F=0 PL:DP:DV 0,21,203:7:0 0,24,219:8:0 0,12,115:4:0 17 1700 . G 0 . DP=20;I16=9,10,0,0,696,25850,0,0,1078,62882,0,0,409,9705,0,0;QS=3,0;MQSB=0.999167;MQ0F=0 PL:DP:DV 0,21,216:7:0 0,24,215:8:0 0,12,110:4:0 17 1701 . T 0 . DP=20;I16=9,11,0,0,711,25539,0,0,1138,66482,0,0,435,10353,0,0;QS=3,0;MQSB=0.997118;MQ0F=0 PL:DP:DV 0,24,207:8:0 0,24,210:8:0 0,12,125:4:0 17 1702 . T 0 . DP=20;I16=8,11,0,0,671,24367,0,0,1078,62882,0,0,410,9712,0,0;QS=3,0;MQSB=0.992359;MQ0F=0 PL:DP:DV 0,24,214:8:0 0,21,193:7:0 0,12,115:4:0 17 1703 . T 0 . DP=20;I16=9,10,0,0,648,22696,0,0,1078,62882,0,0,410,9708,0,0;QS=3,0;MQSB=0.999167;MQ0F=0 PL:DP:DV 0,21,197:7:0 0,24,195:8:0 0,12,114:4:0 17 1704 . T 0 . DP=20;I16=10,10,0,0,711,25771,0,0,1169,69241,0,0,435,10341,0,0;QS=3,0;MQSB=0.931063;MQ0F=0 PL:DP:DV 0,24,208:8:0 0,21,208:7:0 0,15,128:5:0 17 1705 . C 0 . DP=20;I16=10,10,0,0,744,28388,0,0,1169,69241,0,0,436,10312,0,0;QS=3,0;MQSB=0.931063;MQ0F=0 PL:DP:DV 0,24,232:8:0 0,21,213:7:0 0,15,120:5:0 17 1706 . T 0 . DP=20;I16=10,10,0,0,775,30329,0,0,1169,69241,0,0,436,10246,0,0;QS=3,0;MQSB=0.931063;MQ0F=0 PL:DP:DV 0,24,221:8:0 0,21,219:7:0 0,15,145:5:0 17 1707 . C 0 . DP=21;I16=9,11,0,0,724,26998,0,0,1169,69241,0,0,410,9518,0,0;QS=3,0;MQSB=0.916401;MQ0F=0 PL:DP:DV 0,27,235:9:0 0,18,180:6:0 0,15,134:5:0 17 1708 . T 0 . DP=21;I16=9,12,0,0,740,27282,0,0,1229,72841,0,0,410,9430,0,0;QS=3,0;MQSB=0.913101;MQ0F=0 PL:DP:DV 0,30,229:10:0 0,18,187:6:0 0,15,132:5:0 17 1709 . A 0 . DP=21;I16=9,11,0,0,675,23509,0,0,1169,69241,0,0,386,8734,0,0;QS=3,0;MQSB=0.916401;MQ0F=0 PL:DP:DV 0,27,210:9:0 0,18,166:6:0 0,15,131:5:0 17 1710 . C 0 . DP=22;I16=9,13,0,0,755,26817,0,0,1289,76441,0,0,412,9306,0,0;QS=3,0;MQSB=0.910098;MQ0F=0 PL:DP:DV 0,33,236:11:0 0,18,181:6:0 0,15,128:5:0 17 1711 . C 0 . DP=22;I16=9,13,0,0,786,28790,0,0,1289,76441,0,0,413,9223,0,0;QS=3,0;MQSB=0.910098;MQ0F=0 PL:DP:DV 0,33,250:11:0 0,18,169:6:0 0,15,138:5:0 17 1712 . A 0 . DP=22;I16=9,13,0,0,824,31342,0,0,1289,76441,0,0,414,9162,0,0;QS=3,0;MQSB=0.910098;MQ0F=0 PL:DP:DV 0,33,252:11:0 0,18,198:6:0 0,15,136:5:0 17 1713 . G 0 . DP=23;I16=8,14,0,0,823,31285,0,0,1289,76441,0,0,405,8993,0,0;QS=3,0;MQSB=0.892142;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,189:6:0 0,12,118:4:0 17 1714 . A 0 . DP=23;I16=9,13,0,0,763,27439,0,0,1289,76441,0,0,402,8818,0,0;QS=3,0;MQSB=0.910098;MQ0F=0 PL:DP:DV 0,36,234:12:0 0,18,190:6:0 0,12,101:4:0 17 1715 . A 0 . DP=23;I16=9,14,0,0,900,35416,0,0,1349,80041,0,0,414,8878,0,0;QS=3,0;MQSB=0.907354;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,206:6:0 0,15,145:5:0 17 1716 . G 0 . DP=23;I16=9,14,0,0,844,31500,0,0,1349,80041,0,0,414,8822,0,0;QS=3,0;MQSB=0.907354;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,188:6:0 0,15,129:5:0 17 1717 . T 0 . DP=24;I16=9,15,0,0,854,30878,0,0,1409,83641,0,0,414,8794,0,0;QS=3,0;MQSB=0.904837;MQ0F=0 PL:DP:DV 0,39,253:13:0 0,18,185:6:0 0,15,122:5:0 17 1718 . G 0 . DP=24;I16=9,14,0,0,806,29332,0,0,1349,80041,0,0,390,8170,0,0;QS=3,0;MQSB=0.907354;MQ0F=0 PL:DP:DV 0,36,249:12:0 0,18,188:6:0 0,15,124:5:0 17 1719 . C 0 . DP=26;I16=11,14,0,0,857,30717,0,0,1469,87241,0,0,407,8675,0,0;QS=3,0;MQSB=0.929204;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,173:6:0 0,12,98:4:0 17 1720 . C 0 . DP=26;I16=11,14,0,0,898,33274,0,0,1469,87241,0,0,409,8645,0,0;QS=3,0;MQSB=0.929204;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,166:6:0 0,12,99:4:0 17 1721 . C 0 . DP=26;I16=10,14,0,0,846,30642,0,0,1409,83641,0,0,388,8258,0,0;QS=3,0;MQSB=0.919431;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,187:6:0 0,12,119:4:0 17 1722 . T 0 . DP=25;I16=11,13,0,0,876,33054,0,0,1409,83641,0,0,406,8540,0,0;QS=3,0;MQSB=0.931547;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,174:5:0 0,15,123:5:0 17 1723 . T 0 . DP=25;I16=11,14,0,0,835,28861,0,0,1469,87241,0,0,420,8728,0,0;QS=3,0;MQSB=0.929204;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,167:5:0 0,15,130:5:0 17 1724 . C 0 . DP=25;I16=11,14,0,0,903,33735,0,0,1469,87241,0,0,422,8800,0,0;QS=3,0;MQSB=0.929204;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,164:5:0 0,15,139:5:0 17 1725 . C G, 0 . DP=25;I16=11,13,0,1,835,29699,25,625,1409,83641,60,3600,406,8576,18,324;QS=2.95164,0.0483559,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.929204;BQB=1;MQ0F=0 PL:DP:DV 0,20,255,42,255,255:15:1 0,15,153,15,153,153:5:0 0,15,132,15,132,132:5:0 17 1726 . C 0 . DP=25;I16=11,14,0,0,932,35588,0,0,1469,87241,0,0,426,9028,0,0;QS=3,0;MQSB=0.929204;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,165:5:0 0,15,122:5:0 17 1727 . T 0 . DP=25;I16=10,14,0,0,862,32080,0,0,1409,83641,0,0,404,8556,0,0;QS=3,0;MQSB=0.919431;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,167:5:0 0,12,120:4:0 17 1728 . C 0 . DP=25;I16=11,14,0,0,915,33845,0,0,1469,87241,0,0,429,9173,0,0;QS=3,0;MQSB=0.929204;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,156:5:0 0,15,144:5:0 17 1729 . C 0 . DP=25;I16=10,14,0,0,919,35797,0,0,1409,83641,0,0,406,8668,0,0;QS=3,0;MQSB=0.919431;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,142:5:0 0,12,128:4:0 17 1730 . T 0 . DP=24;I16=10,14,0,0,849,30711,0,0,1409,83641,0,0,433,9393,0,0;QS=3,0;MQSB=0.919431;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,138:4:0 0,15,148:5:0 17 1731 . C 0 . DP=24;I16=10,14,0,0,907,34875,0,0,1409,83641,0,0,434,9472,0,0;QS=3,0;MQSB=0.919431;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,123:4:0 0,15,140:5:0 17 1732 . A 0 . DP=24;I16=11,13,0,0,816,28582,0,0,1409,83641,0,0,436,9580,0,0;QS=3,0;MQSB=0.931547;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,123:4:0 0,15,143:5:0 17 1733 . C 0 . DP=25;I16=12,13,0,0,869,31451,0,0,1469,87241,0,0,438,9666,0,0;QS=3,0;MQSB=0.939413;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,116:4:0 0,15,136:5:0 17 1734 . C 0 . DP=25;I16=12,13,0,0,926,35482,0,0,1469,87241,0,0,440,9730,0,0;QS=3,0;MQSB=0.939413;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,122:4:0 0,15,141:5:0 17 1735 . T 0 . DP=26;I16=13,13,0,0,935,35169,0,0,1529,90841,0,0,442,9822,0,0;QS=3,0;MQSB=0.945959;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,112:4:0 0,15,148:5:0 17 1736 . G 0 . DP=25;I16=11,11,0,0,829,31469,0,0,1289,76441,0,0,380,8416,0,0;QS=3,0;MQSB=0.936864;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,72:2:0 0,12,118:4:0 17 1737 . A 0 . DP=25;I16=12,12,0,0,842,30606,0,0,1409,83641,0,0,422,9312,0,0;QS=3,0;MQSB=0.941765;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,76:2:0 0,15,131:5:0 17 1738 . C 0 . DP=24;I16=12,12,0,0,835,30251,0,0,1409,83641,0,0,450,10010,0,0;QS=3,0;MQSB=0.941765;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,78:3:0 0,15,130:5:0 17 1739 . C 0 . DP=23;I16=10,12,0,0,831,32123,0,0,1289,76441,0,0,428,9432,0,0;QS=3,0;MQSB=0.924723;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,55:2:0 0,12,135:4:0 17 1740 . A 0 . DP=23;I16=11,12,0,0,774,27126,0,0,1349,80041,0,0,456,10126,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,56:2:0 0,15,139:5:0 17 1741 . C 0 . DP=23;I16=11,12,0,0,850,32314,0,0,1349,80041,0,0,459,10217,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,70:2:0 0,15,136:5:0 17 1742 . T 0 . DP=23;I16=11,12,0,0,866,33204,0,0,1349,80041,0,0,462,10330,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,72:2:0 0,15,144:5:0 17 1743 . C 0 . DP=23;I16=11,12,0,0,894,35176,0,0,1349,80041,0,0,464,10414,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,74:2:0 0,15,138:5:0 17 1744 . T 0 . DP=23;I16=11,11,0,0,857,33579,0,0,1289,76441,0,0,459,10469,0,0;QS=3,0;MQSB=0.936864;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,80:2:0 0,15,156:5:0 17 1745 . G 0 . DP=23;I16=11,12,0,0,882,34572,0,0,1349,80041,0,0,464,10442,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,78:2:0 0,15,142:5:0 17 1746 . G 0 . DP=24;I16=12,11,0,0,836,31102,0,0,1349,80041,0,0,456,10312,0,0;QS=3,0;MQSB=0.944319;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,69:2:0 0,18,151:6:0 17 1747 . G 0 . DP=24;I16=12,11,0,0,839,31729,0,0,1349,80041,0,0,455,10239,0,0;QS=3,0;MQSB=0.944319;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,75:2:0 0,18,154:6:0 17 1748 . G 0 . DP=24;I16=11,12,0,0,843,31857,0,0,1349,80041,0,0,434,9664,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,3,42:1:0 0,18,154:6:0 17 1749 . A 0 . DP=25;I16=13,11,0,0,864,31748,0,0,1409,83641,0,0,451,10063,0,0;QS=3,0;MQSB=0.950498;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,72:2:0 0,21,172:7:0 17 1750 . A 0 . DP=25;I16=13,12,0,0,905,33667,0,0,1469,87241,0,0,451,10013,0,0;QS=3,0;MQSB=0.948139;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,56:2:0 0,21,187:7:0 17 1751 . A 0 . DP=25;I16=13,12,0,0,908,33658,0,0,1469,87241,0,0,449,9987,0,0;QS=3,0;MQSB=0.948139;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,67:2:0 0,21,183:7:0 17 1752 . T 0 . DP=23;I16=12,11,0,0,838,31088,0,0,1380,82800,0,0,449,9987,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,71:2:0 0,18,170:6:0 17 1753 . C 0 . DP=23;I16=12,11,0,0,869,33595,0,0,1380,82800,0,0,448,9960,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,77:2:0 0,18,163:6:0 17 1754 . C 0 . DP=23;I16=11,11,0,0,830,31628,0,0,1320,79200,0,0,431,9699,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,3,42:1:0 0,18,163:6:0 17 1755 . C 0 . DP=23;I16=12,11,0,0,856,33082,0,0,1380,82800,0,0,446,9972,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,61:2:0 0,18,165:6:0 17 1756 . T 0 . DP=22;I16=11,11,0,0,858,33720,0,0,1320,79200,0,0,445,9961,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,77:2:0 0,18,179:6:0 17 1757 . C 0 . DP=22;I16=11,11,0,0,869,34651,0,0,1320,79200,0,0,444,9972,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,78:2:0 0,18,176:6:0 17 1758 . A 0 . DP=22;I16=11,11,0,0,865,34205,0,0,1320,79200,0,0,442,9954,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,81:2:0 0,18,177:6:0 17 1759 . G 0 . DP=22;I16=11,11,0,0,833,31965,0,0,1320,79200,0,0,439,9905,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,77:2:0 0,18,159:6:0 17 1760 . C 0 . DP=22;I16=11,11,0,0,849,33441,0,0,1320,79200,0,0,436,9874,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,79:2:0 0,18,159:6:0 17 1761 . A 0 . DP=22;I16=11,11,0,0,730,25766,0,0,1320,79200,0,0,432,9810,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,75:2:0 0,18,153:6:0 17 1762 . C 0 . DP=21;I16=11,10,0,0,767,28667,0,0,1260,75600,0,0,429,9761,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,76:2:0 0,18,160:6:0 17 1763 . C 0 . DP=21;I16=11,10,0,0,789,30115,0,0,1260,75600,0,0,426,9726,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,72:2:0 0,18,162:6:0 17 1764 . C 0 . DP=21;I16=11,10,0,0,821,32443,0,0,1260,75600,0,0,423,9705,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,72:2:0 0,18,167:6:0 17 1765 . T 0 . DP=21;I16=11,10,0,0,814,31746,0,0,1260,75600,0,0,420,9698,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,83:2:0 0,18,179:6:0 17 1766 . C 0 . DP=21;I16=11,10,0,0,796,30564,0,0,1260,75600,0,0,417,9705,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,71:2:0 0,18,162:6:0 17 1767 . C 0 . DP=21;I16=11,10,0,0,779,29559,0,0,1260,75600,0,0,414,9726,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,73:2:0 0,18,163:6:0 17 1768 . C 0 . DP=21;I16=11,10,0,0,798,30670,0,0,1260,75600,0,0,411,9761,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,68:2:0 0,18,160:6:0 17 1769 . T 0 . DP=21;I16=11,10,0,0,819,32179,0,0,1260,75600,0,0,406,9712,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,77:2:0 0,18,184:6:0 17 1770 . G 0 . DP=19;I16=11,8,0,0,699,26453,0,0,1140,68400,0,0,403,9679,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,6,68:2:0 0,18,158:6:0 17 1771 . A 0 . DP=18;I16=10,8,0,0,707,27853,0,0,1080,64800,0,0,401,9659,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,3,42:1:0 0,18,177:6:0 17 1772 . G 0 . DP=18;I16=10,8,0,0,669,25513,0,0,1080,64800,0,0,398,9600,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,3,38:1:0 0,18,159:6:0 17 1773 . C 0 . DP=17;I16=10,7,0,0,675,26897,0,0,1020,61200,0,0,396,9550,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,3,39:1:0 0,18,172:6:0 17 1774 . A 0 . DP=17;I16=10,7,0,0,653,25153,0,0,1020,61200,0,0,394,9508,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,3,39:1:0 0,18,172:6:0 17 1775 . T 0 . DP=17;I16=10,7,0,0,605,22253,0,0,1020,61200,0,0,391,9423,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,3,38:1:0 0,18,156:6:0 17 1776 . A 0 . DP=17;I16=10,7,0,0,610,22124,0,0,1020,61200,0,0,388,9344,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,254:10:0 0,3,35:1:0 0,18,166:6:0 17 1777 . C 0 . DP=18;I16=11,7,0,0,661,24975,0,0,1080,64800,0,0,385,9271,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,3,35:1:0 0,18,158:6:0 17 1778 . C 0 . DP=18;I16=11,7,0,0,680,25970,0,0,1080,64800,0,0,383,9205,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,3,31:1:0 0,18,172:6:0 17 1779 . C 0 . DP=18;I16=11,7,0,0,703,27663,0,0,1080,64800,0,0,381,9147,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,3,34:1:0 0,18,174:6:0 17 1780 . T 0 . DP=18;I16=11,7,0,0,672,26000,0,0,1080,64800,0,0,378,9048,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,3,33:1:0 0,18,177:6:0 17 1781 . A 0 . DP=19;I16=10,6,0,0,579,21377,0,0,960,57600,0,0,328,7804,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,0,0:0:0 0,15,160:5:0 17 1782 . C 0 . DP=20;I16=11,9,0,0,715,26603,0,0,1200,72000,0,0,382,8892,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,47:2:0 0,18,156:6:0 17 1783 . T 0 . DP=20;I16=11,9,0,0,755,29057,0,0,1200,72000,0,0,381,8743,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,68:2:0 0,18,184:6:0 17 1784 . C 0 . DP=20;I16=10,9,0,0,723,28017,0,0,1140,68400,0,0,360,8212,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,62:2:0 0,15,151:5:0 17 1785 . T 0 . DP=20;I16=10,10,0,0,766,29878,0,0,1200,72000,0,0,359,8089,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,66:2:0 0,15,168:5:0 17 1786 . G 0 . DP=22;I16=11,11,0,0,841,32323,0,0,1320,79200,0,0,359,7985,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,68:2:0 0,15,158:5:0 17 1787 . G 0 . DP=22;I16=11,11,0,0,806,30294,0,0,1320,79200,0,0,361,7903,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,68:2:0 0,15,148:5:0 17 1788 . C 0 . DP=22;I16=11,11,0,0,858,33674,0,0,1320,79200,0,0,362,7796,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,67:2:0 0,15,158:5:0 17 1789 . A 0 . DP=23;I16=11,12,0,0,797,28705,0,0,1380,82800,0,0,363,7715,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,69:2:0 0,15,157:5:0 17 1790 . C 0 . DP=23;I16=11,12,0,0,852,31816,0,0,1380,82800,0,0,365,7661,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,63:2:0 0,15,153:5:0 17 1791 . A 0 . DP=23;I16=11,12,0,0,830,30484,0,0,1380,82800,0,0,367,7635,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,61:2:0 0,15,155:5:0 17 1792 . A 0 . DP=23;I16=11,12,0,0,862,32760,0,0,1380,82800,0,0,368,7588,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,67:2:0 0,15,161:5:0 17 1793 . G 0 . DP=23;I16=11,12,0,0,813,29603,0,0,1380,82800,0,0,369,7571,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,61:2:0 0,15,126:5:0 17 1794 . C 0 . DP=21;I16=9,12,0,0,736,26472,0,0,1260,75600,0,0,370,7484,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,48:2:0 0,9,96:3:0 17 1795 . C 0 . DP=21;I16=9,12,0,0,763,28807,0,0,1260,75600,0,0,371,7427,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,45:2:0 0,9,100:3:0 17 1796 . C 0 . DP=21;I16=9,12,0,0,782,29718,0,0,1260,75600,0,0,372,7400,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,52:2:0 0,9,102:3:0 17 1797 . A 0 . DP=21;I16=9,12,0,0,704,25190,0,0,1260,75600,0,0,373,7403,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,59:2:0 0,9,92:3:0 17 1798 . C 0 . DP=21;I16=9,12,0,0,759,28119,0,0,1260,75600,0,0,374,7436,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,49:2:0 0,9,99:3:0 17 1799 . C 0 . DP=22;I16=9,13,0,0,796,29520,0,0,1320,79200,0,0,375,7499,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,56:2:0 0,12,131:4:0 17 1800 . C 0 . DP=22;I16=9,13,0,0,866,34352,0,0,1320,79200,0,0,376,7542,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,63:2:0 0,12,138:4:0 17 1801 . T 0 . DP=22;I16=9,13,0,0,838,32282,0,0,1320,79200,0,0,377,7615,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,63:2:0 0,12,150:4:0 17 1802 . G 0 . DP=22;I16=9,13,0,0,844,32894,0,0,1320,79200,0,0,378,7718,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,60:2:0 0,12,137:4:0 17 1803 . C 0 . DP=22;I16=9,13,0,0,837,32691,0,0,1320,79200,0,0,377,7751,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,60:2:0 0,12,143:4:0 17 1804 . A 0 . DP=23;I16=9,14,0,0,793,28517,0,0,1380,82800,0,0,376,7814,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,54:2:0 0,12,144:4:0 17 1805 . A 0 . DP=23;I16=9,14,0,0,810,29148,0,0,1380,82800,0,0,376,7908,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,67:2:0 0,12,138:4:0 17 1806 . A 0 . DP=23;I16=9,14,0,0,816,29932,0,0,1380,82800,0,0,376,8034,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,61:2:0 0,12,139:4:0 17 1807 . G 0 . DP=22;I16=9,13,0,0,837,32299,0,0,1320,79200,0,0,375,8091,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,67:2:0 0,12,142:4:0 17 1808 . C 0 . DP=21;I16=9,11,0,0,705,25519,0,0,1200,72000,0,0,354,7716,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,53:2:0 0,12,140:4:0 17 1809 . C 0 . DP=22;I16=10,12,0,0,789,29123,0,0,1320,79200,0,0,371,8091,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,55:2:0 0,12,143:4:0 17 1810 . C 0 . DP=21;I16=10,11,0,0,754,27902,0,0,1260,75600,0,0,370,8084,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,51:2:0 0,12,145:4:0 17 1811 . C 0 . DP=22;I16=11,11,0,0,837,32353,0,0,1320,79200,0,0,368,8056,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,61:2:0 0,12,146:4:0 17 1812 . T 0 . DP=22;I16=11,11,0,0,830,31782,0,0,1320,79200,0,0,365,7955,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,60:2:0 0,12,151:4:0 17 1813 . G 0 . DP=21;I16=11,10,0,0,789,29971,0,0,1260,75600,0,0,363,7879,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,63:2:0 0,12,135:4:0 17 1814 . A 0 . DP=21;I16=11,10,0,0,780,29356,0,0,1260,75600,0,0,361,7827,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,63:2:0 0,12,148:4:0 17 1815 . G 0 . DP=21;I16=11,10,0,0,742,27064,0,0,1260,75600,0,0,358,7748,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,56:2:0 0,12,135:4:0 17 1816 . G 0 . DP=21;I16=11,10,0,0,702,24670,0,0,1260,75600,0,0,355,7691,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,49:2:0 0,12,124:4:0 17 1817 . C 0 . DP=20;I16=11,9,0,0,701,25263,0,0,1200,72000,0,0,353,7655,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,58:2:0 0,12,134:4:0 17 1818 . C 0 . DP=20;I16=11,8,0,0,665,23987,0,0,1140,68400,0,0,326,7014,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,53:2:0 0,12,132:4:0 17 1819 . C 0 . DP=18;I16=9,9,0,0,580,20092,0,0,1080,64800,0,0,351,7641,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,38:2:0 0,12,124:4:0 17 1820 . G 0 . DP=18;I16=9,9,0,0,589,19883,0,0,1080,64800,0,0,351,7659,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,58:2:0 0,12,117:4:0 17 1821 . C 0 . DP=18;I16=9,9,0,0,619,22131,0,0,1080,64800,0,0,351,7693,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,60:2:0 0,12,131:4:0 17 1822 . C 0 . DP=18;I16=9,9,0,0,655,24177,0,0,1080,64800,0,0,350,7694,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,58:2:0 0,12,131:4:0 17 1823 . C 0 . DP=19;I16=10,9,0,0,703,26765,0,0,1140,68400,0,0,349,7713,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,62:2:0 0,12,138:4:0 17 1824 . T 0 . DP=19;I16=10,9,0,0,714,27628,0,0,1140,68400,0,0,349,7751,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,69:2:0 0,12,149:4:0 17 1825 . G 0 . DP=19;I16=10,9,0,0,682,24804,0,0,1140,68400,0,0,347,7709,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,61:2:0 0,12,129:4:0 17 1826 . T 0 . DP=19;I16=10,9,0,0,643,22525,0,0,1140,68400,0,0,345,7687,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,62:2:0 0,12,141:4:0 17 1827 . G 0 . DP=19;I16=10,9,0,0,722,27844,0,0,1140,68400,0,0,343,7685,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,66:2:0 0,12,134:4:0 17 1828 . G 0 . DP=18;I16=10,8,0,0,619,21719,0,0,1080,64800,0,0,342,7702,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,61:2:0 0,9,100:3:0 17 1829 . C 0 . DP=19;I16=9,9,0,0,576,19228,0,0,1080,64800,0,0,323,7413,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,59:3:0 0,9,103:3:0 17 1830 . G 0 . DP=19;I16=9,9,0,0,582,19586,0,0,1080,64800,0,0,321,7379,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,82:3:0 0,9,80:3:0 17 1831 . T 0 . DP=20;I16=10,8,0,0,605,21015,0,0,1080,64800,0,0,294,6736,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,106:4:0 0,9,101:3:0 17 1832 . C 0 . DP=19;I16=9,9,0,0,675,25747,0,0,1080,64800,0,0,319,7359,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,110:4:0 0,9,105:3:0 17 1833 . T 0 . DP=18;I16=8,8,0,0,565,20733,0,0,960,57600,0,0,295,6747,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,248:10:0 0,9,105:3:0 0,9,106:3:0 17 1834 . C 0 . DP=19;I16=9,9,0,0,654,24424,0,0,1037,61489,0,0,321,7399,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,104:4:0 0,9,107:3:0 17 1835 . T 0 . DP=18;I16=9,9,0,0,641,23505,0,0,1037,61489,0,0,347,7965,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,123:4:0 0,9,115:3:0 17 1836 . C 0 . DP=18;I16=9,9,0,0,657,24427,0,0,1037,61489,0,0,350,8016,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,127:4:0 0,9,108:3:0 17 1837 . C 0 . DP=18;I16=9,9,0,0,586,20044,0,0,1037,61489,0,0,352,8030,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,33,233:11:0 0,12,114:4:0 0,9,108:3:0 17 1838 . C 0 . DP=18;I16=9,9,0,0,655,24607,0,0,1037,61489,0,0,354,8056,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,120:4:0 0,9,107:3:0 17 1839 . T 0 . DP=18;I16=9,7,0,0,557,20543,0,0,917,54289,0,0,321,7369,0,0;QS=3,0;MQSB=0.933674;MQ0F=0 PL:DP:DV 0,30,231:10:0 0,9,103:3:0 0,9,119:3:0 17 1840 . C 0 . DP=18;I16=9,9,0,0,637,23295,0,0,1037,61489,0,0,358,8144,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,116:4:0 0,9,109:3:0 17 1841 . C 0 . DP=18;I16=9,9,0,0,622,22458,0,0,1037,61489,0,0,360,8206,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,33,252:11:0 0,12,121:4:0 0,9,108:3:0 17 1842 . C 0 . DP=18;I16=9,9,0,0,672,26064,0,0,1037,61489,0,0,362,8280,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,124:4:0 0,9,108:3:0 17 1843 . T 0 . DP=18;I16=9,9,0,0,630,22746,0,0,1037,61489,0,0,364,8366,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,33,243:11:0 0,12,131:4:0 0,9,107:3:0 17 1844 . T 0 . DP=18;I16=9,9,0,0,618,21618,0,0,1037,61489,0,0,366,8464,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,33,240:11:0 0,12,137:4:0 0,9,98:3:0 17 1845 . G 0 . DP=18;I16=9,9,0,0,644,23976,0,0,1037,61489,0,0,368,8574,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,138:4:0 0,9,82:3:0 17 1846 . C 0 . DP=18;I16=9,9,0,0,687,26809,0,0,1037,61489,0,0,370,8696,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,129:4:0 0,9,100:3:0 17 1847 . T 0 . DP=18;I16=9,9,0,0,675,25529,0,0,1037,61489,0,0,373,8829,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,132:4:0 0,6,76:2:0 17 1848 . G 0 . DP=18;I16=9,9,0,0,597,20845,0,0,1037,61489,0,0,377,8973,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,118:4:0 0,6,64:2:0 17 1849 . T 0 . DP=20;I16=9,10,0,0,614,20770,0,0,1097,65089,0,0,380,9078,0,0;QS=3,0;MQSB=0.920044;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,119:4:0 0,6,71:2:0 17 1850 . C 0 . DP=19;I16=8,11,0,0,684,25154,0,0,1097,65089,0,0,409,9769,0,0;QS=3,0;MQSB=0.902014;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,120:4:0 0,3,37:1:0 17 1851 . A 0 . DP=20;I16=9,11,0,0,738,27838,0,0,1157,68689,0,0,413,9847,0,0;QS=3,0;MQSB=0.916401;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,143:4:0 0,3,41:1:0 17 1852 . G 0 . DP=19;I16=8,11,0,0,639,22239,0,0,1097,65089,0,0,392,9264,0,0;QS=3,0;MQSB=0.902014;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,117:4:0 0,3,36:1:0 17 1853 . G 0 . DP=20;I16=9,11,0,0,627,20873,0,0,1157,68689,0,0,396,9322,0,0;QS=3,0;MQSB=0.916401;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,127:5:0 0,3,38:1:0 17 1854 . A 0 . DP=22;I16=10,11,0,0,674,22428,0,0,1217,72289,0,0,401,9397,0,0;QS=3,0;MQSB=0.927743;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,145:5:0 0,3,38:1:0 17 1855 . C 0 . DP=22;I16=10,12,0,0,771,27949,0,0,1277,75889,0,0,407,9441,0,0;QS=3,0;MQSB=0.924723;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,132:5:0 0,3,37:1:0 17 1856 . A 0 . DP=22;I16=10,12,0,0,806,30230,0,0,1277,75889,0,0,412,9456,0,0;QS=3,0;MQSB=0.924723;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,146:5:0 0,3,41:1:0 17 1857 . G 0 . DP=22;I16=10,12,0,0,699,23919,0,0,1277,75889,0,0,416,9442,0,0;QS=3,0;MQSB=0.924723;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,134:5:0 0,3,35:1:0 17 1858 . T 0 . DP=23;I16=10,13,0,0,773,26797,0,0,1337,79489,0,0,419,9399,0,0;QS=3,0;MQSB=0.921963;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,15,148:5:0 0,3,37:1:0 17 1859 . G 0 . DP=23;I16=10,13,0,0,798,28594,0,0,1337,79489,0,0,423,9379,0,0;QS=3,0;MQSB=0.921963;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,15,146:5:0 0,3,36:1:0 17 1860 . G 0 . DP=23;I16=10,13,0,0,726,23842,0,0,1337,79489,0,0,425,9283,0,0;QS=3,0;MQSB=0.921963;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,15,126:5:0 0,3,33:1:0 17 1861 . T 0 . DP=23;I16=10,13,0,0,741,24793,0,0,1337,79489,0,0,425,9113,0,0;QS=3,0;MQSB=0.921963;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,15,143:5:0 0,3,39:1:0 17 1862 . C 0 . DP=23;I16=10,12,0,0,738,25732,0,0,1277,75889,0,0,403,8487,0,0;QS=3,0;MQSB=0.924723;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,135:5:0 0,3,34:1:0 17 1863 . C 0 . DP=24;I16=10,13,0,0,804,29186,0,0,1337,79489,0,0,400,8232,0,0;QS=3,0;MQSB=0.921963;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,15,133:5:0 0,3,36:1:0 17 1864 . T 0 . DP=24;I16=11,11,0,0,766,27572,0,0,1277,75889,0,0,392,8174,0,0;QS=3,0;MQSB=0.936864;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,125:4:0 0,3,37:1:0 17 1865 . G 0 . DP=24;I16=11,13,0,0,860,31394,0,0,1397,83089,0,0,425,8621,0,0;QS=3,0;MQSB=0.931547;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,15,154:5:0 0,3,38:1:0 17 1866 . G 0 . DP=24;I16=11,13,0,0,795,27503,0,0,1397,83089,0,0,425,8551,0,0;QS=3,0;MQSB=0.931547;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,15,152:5:0 0,3,36:1:0 17 1867 . C 0 . DP=24;I16=9,13,0,0,726,24886,0,0,1320,79200,0,0,375,7263,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,122:5:0 0,3,38:1:0 17 1868 . C 0 . DP=24;I16=11,12,0,0,857,32293,0,0,1337,79489,0,0,411,8311,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,12,118:4:0 0,3,41:1:0 17 1869 . A T, 0 . DP=24;I16=6,9,5,4,531,19001,268,8660,857,50689,540,32400,273,5667,152,2866;QS=1.4724,1.5276,0;VDB=0.928022;SGB=-11.9537;RPB=0.984127;MQB=0.96464;MQSB=0.931547;BQB=0.359155;MQ0F=0 PL:DP:DV 115,0,224,148,245,255:18:7 16,0,104,28,107,128:5:1 42,3,0,42,3,42:1:1 17 1870 . C 0 . DP=25;I16=11,13,0,0,804,27826,0,0,1397,83089,0,0,425,8591,0,0;QS=3,0;MQSB=0.931547;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,15,127:5:0 0,3,36:1:0 17 1871 . C 0 . DP=25;I16=11,14,0,0,761,24187,0,0,1457,86689,0,0,428,8690,0,0;QS=3,0;MQSB=0.929204;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,128:6:0 0,3,29:1:0 17 1872 . G 0 . DP=25;I16=10,14,0,0,763,25437,0,0,1397,83089,0,0,425,8803,0,0;QS=3,0;MQSB=0.919431;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,132:6:0 0,3,43:1:0 17 1873 . G 0 . DP=26;I16=11,15,0,0,790,25548,0,0,1517,90289,0,0,429,8931,0,0;QS=3,0;MQSB=0.927041;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,151:7:0 0,3,43:1:0 17 1874 . G 0 . DP=27;I16=11,16,0,0,861,29013,0,0,1577,93889,0,0,430,9076,0,0;QS=3,0;MQSB=0.925036;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,24,172:8:0 0,3,43:1:0 17 1875 . G 0 . DP=26;I16=11,15,0,0,800,26216,0,0,1517,90289,0,0,431,9155,0,0;QS=3,0;MQSB=0.927041;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,168:7:0 0,3,42:1:0 17 1876 . C 0 . DP=26;I16=10,15,0,0,894,32714,0,0,1457,86689,0,0,432,9268,0,0;QS=3,0;MQSB=0.9171;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,171:7:0 0,3,41:1:0 17 1877 . T 0 . DP=25;I16=9,15,0,0,848,30804,0,0,1397,83089,0,0,409,8787,0,0;QS=3,0;MQSB=0.904837;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,163:6:0 0,3,42:1:0 17 1878 . C 0 . DP=25;I16=10,15,0,0,906,33478,0,0,1457,86689,0,0,434,9488,0,0;QS=3,0;MQSB=0.9171;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,166:7:0 0,3,40:1:0 17 1879 . A 0 . DP=26;I16=10,16,0,0,911,32761,0,0,1517,90289,0,0,433,9543,0,0;QS=3,0;MQSB=0.914947;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,191:8:0 0,3,40:1:0 17 1880 . C 0 . DP=26;I16=10,16,0,0,790,24876,0,0,1517,90289,0,0,431,9527,0,0;QS=3,0;MQSB=0.914947;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,152:8:0 0,3,31:1:0 17 1881 . G 0 . DP=27;I16=10,15,0,0,782,25352,0,0,1500,90000,0,0,380,8288,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,144:7:0 0,3,42:1:0 17 1882 . G 0 . DP=28;I16=12,15,0,0,937,33215,0,0,1577,93889,0,0,406,8952,0,0;QS=3,0;MQSB=0.935229;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,164:7:0 0,3,42:1:0 17 1883 . A 0 . DP=29;I16=14,15,0,0,998,35394,0,0,1697,101089,0,0,434,9646,0,0;QS=3,0;MQSB=0.947838;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,24,180:8:0 0,3,43:1:0 17 1884 . G 0 . DP=29;I16=14,15,0,0,1036,37926,0,0,1697,101089,0,0,437,9647,0,0;QS=3,0;MQSB=0.947838;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,24,202:8:0 0,3,42:1:0 17 1885 . C 0 . DP=28;I16=14,13,0,0,925,32305,0,0,1577,93889,0,0,430,9560,0,0;QS=3,0;MQSB=0.951472;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,182:7:0 0,3,42:1:0 17 1886 . C 0 . DP=26;I16=13,13,0,0,826,27194,0,0,1517,90289,0,0,447,9745,0,0;QS=3,0;MQSB=0.945959;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,166:8:0 0,3,32:1:0 17 1887 . G 0 . DP=26;I16=12,13,0,0,798,26554,0,0,1500,90000,0,0,428,9212,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,24,185:8:0 0,3,40:1:0 17 1888 . C 0 . DP=26;I16=13,13,0,0,901,32343,0,0,1517,90289,0,0,459,9957,0,0;QS=3,0;MQSB=0.945959;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,178:8:0 0,3,39:1:0 17 1889 . C 0 . DP=25;I16=13,11,0,0,825,29127,0,0,1397,83089,0,0,444,9612,0,0;QS=3,0;MQSB=0.950498;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,187:7:0 0,3,37:1:0 17 1890 . C 0 . DP=25;I16=13,12,0,0,913,34029,0,0,1457,86689,0,0,471,10173,0,0;QS=3,0;MQSB=0.948139;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,24,198:8:0 0,3,40:1:0 17 1891 . T 0 . DP=25;I16=13,12,0,0,912,34028,0,0,1457,86689,0,0,477,10317,0,0;QS=3,0;MQSB=0.948139;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,24,212:8:0 0,3,39:1:0 17 1892 . G 0 . DP=25;I16=12,12,0,0,885,33149,0,0,1397,83089,0,0,458,9860,0,0;QS=3,0;MQSB=0.941765;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,166:7:0 0,3,37:1:0 17 1893 . T 0 . DP=26;I16=14,12,0,0,920,33494,0,0,1517,90289,0,0,489,10677,0,0;QS=3,0;MQSB=0.953497;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,24,205:8:0 0,6,72:2:0 17 1894 . G 0 . DP=26;I16=14,12,0,0,934,35048,0,0,1517,90289,0,0,495,10843,0,0;QS=3,0;MQSB=0.953497;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,24,188:8:0 0,6,75:2:0 17 1895 . C 0 . DP=26;I16=13,12,0,0,882,31980,0,0,1500,90000,0,0,476,10408,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,24,180:8:0 0,6,73:2:0 17 1896 . C 0 . DP=26;I16=14,11,0,0,827,28179,0,0,1457,86689,0,0,482,10622,0,0;QS=3,0;MQSB=0.955682;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,154:7:0 0,6,72:2:0 17 1897 . G 0 . DP=26;I16=13,11,0,0,763,25169,0,0,1397,83089,0,0,486,10856,0,0;QS=3,0;MQSB=0.950498;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,164:7:0 0,3,27:1:0 17 1898 . T 0 . DP=27;I16=14,11,0,0,846,29526,0,0,1500,90000,0,0,492,11072,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,24,187:8:0 0,6,62:2:0 17 1899 . G 0 . DP=27;I16=14,11,0,0,875,32007,0,0,1500,90000,0,0,497,11213,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,24,188:8:0 0,6,61:2:0 17 1900 . T 0 . DP=26;I16=14,11,0,0,850,29882,0,0,1500,90000,0,0,501,11329,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,24,185:8:0 0,6,69:2:0 17 1901 . A 0 . DP=27;I16=15,10,0,0,842,29298,0,0,1457,86689,0,0,505,11469,0,0;QS=3,0;MQSB=0.962269;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,190:7:0 0,6,68:2:0 17 1902 . C 0 . DP=27;I16=15,12,0,0,912,31970,0,0,1577,93889,0,0,537,12267,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,184:8:0 0,6,60:2:0 17 1903 . C 0 . DP=27;I16=15,12,0,0,959,34645,0,0,1577,93889,0,0,542,12462,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,201:8:0 0,6,63:2:0 17 1904 . T 0 . DP=27;I16=16,11,0,0,1017,38757,0,0,1577,93889,0,0,548,12682,0,0;QS=3,0;MQSB=0.96384;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,221:8:0 0,6,77:2:0 17 1905 . C 0 . DP=27;I16=16,11,0,0,985,36561,0,0,1577,93889,0,0,553,12827,0,0;QS=3,0;MQSB=0.96384;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,211:8:0 0,6,69:2:0 17 1906 . T 0 . DP=27;I16=16,11,0,0,1018,39242,0,0,1577,93889,0,0,558,12998,0,0;QS=3,0;MQSB=0.96384;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,207:8:0 0,6,75:2:0 17 1907 . G 0 . DP=27;I16=15,11,0,0,935,34679,0,0,1517,90289,0,0,535,12419,0,0;QS=3,0;MQSB=0.960078;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,187:7:0 0,6,68:2:0 17 1908 . A 0 . DP=28;I16=16,12,0,0,1023,38221,0,0,1637,97489,0,0,561,13063,0,0;QS=3,0;MQSB=0.96195;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,24,215:8:0 0,6,67:2:0 17 1909 . G 0 . DP=27;I16=15,12,0,0,1002,38398,0,0,1577,93889,0,0,561,12953,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,206:8:0 0,6,73:2:0 17 1910 . C 0 . DP=27;I16=14,10,0,0,855,31251,0,0,1440,86400,0,0,502,11588,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,179:7:0 0,6,71:2:0 17 1911 . C 0 . DP=27;I16=15,12,0,0,967,35429,0,0,1577,93889,0,0,561,12793,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,206:8:0 0,6,71:2:0 17 1912 . C 0 . DP=27;I16=15,12,0,0,1024,39272,0,0,1577,93889,0,0,561,12743,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,217:8:0 0,6,73:2:0 17 1913 . T 0 . DP=27;I16=15,12,0,0,1028,39768,0,0,1577,93889,0,0,561,12713,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,228:8:0 0,6,77:2:0 17 1914 . C 0 . DP=27;I16=15,12,0,0,1010,38762,0,0,1577,93889,0,0,561,12703,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,221:8:0 0,6,75:2:0 17 1915 . T C, 0 . DP=27;I16=14,12,1,0,974,37184,16,256,1560,93600,17,289,543,12389,18,324;QS=2.97351,0.0264901,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.958048;BQB=1;MQ0F=0 PL:DP:DV 0,34,255,48,255,255:17:1 0,24,231,24,231,231:8:0 0,6,60,6,60,60:2:0 17 1916 . G T, 0 . DP=27;I16=14,12,1,0,991,38291,15,225,1560,93600,17,289,544,12454,17,289;QS=2.97581,0.0241935,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.958048;BQB=1;MQ0F=0 PL:DP:DV 0,35,255,48,255,255:17:1 0,24,226,24,226,226:8:0 0,6,69,6,69,69:2:0 17 1917 . C 0 . DP=27;I16=15,12,0,0,1030,39740,0,0,1577,93889,0,0,561,12793,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,214:8:0 0,6,73:2:0 17 1918 . A 0 . DP=27;I16=15,11,0,0,963,36201,0,0,1517,90289,0,0,542,12502,0,0;QS=3,0;MQSB=0.960078;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,24,217:8:0 0,6,72:2:0 17 1919 . C 0 . DP=27;I16=15,12,0,0,987,36651,0,0,1577,93889,0,0,560,12902,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,214:8:0 0,6,72:2:0 17 1920 . A T, 0 . DP=29;I16=15,12,0,1,1007,38337,14,196,1546,91130,60,3600,538,12518,21,441;QS=2.97647,0.0235294,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.999735;BQB=1;MQ0F=0 PL:DP:DV 0,36,255,48,255,255:17:1 0,24,225,24,225,225:8:0 0,9,101,9,101,101:3:0 17 1921 . G 0 . DP=30;I16=15,14,0,0,1059,39815,0,0,1666,98330,0,0,542,12474,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,182:7:0 0,12,132:4:0 17 1922 . T 0 . DP=29;I16=14,14,0,0,1013,37513,0,0,1649,98041,0,0,532,12418,0,0;QS=3,0;MQSB=0.949591;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,197:7:0 0,12,134:4:0 17 1923 . G 0 . DP=28;I16=14,14,0,0,1034,38974,0,0,1606,94730,0,0,545,12629,0,0;QS=3,0;MQSB=0.999736;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,188:7:0 0,12,126:4:0 17 1924 . C 0 . DP=27;I16=13,13,0,0,965,36587,0,0,1486,87530,0,0,521,12017,0,0;QS=3,0;MQSB=0.999671;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,183:7:0 0,12,116:4:0 17 1925 . C 0 . DP=27;I16=14,13,0,0,987,37021,0,0,1546,91130,0,0,546,12626,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,187:7:0 0,12,124:4:0 17 1926 . T 0 . DP=27;I16=14,13,0,0,1031,40057,0,0,1546,91130,0,0,545,12581,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,197:7:0 0,12,141:4:0 17 1927 . T 0 . DP=27;I16=13,13,0,0,959,35773,0,0,1529,90841,0,0,538,12522,0,0;QS=3,0;MQSB=0.945959;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,191:7:0 0,12,133:4:0 17 1928 . C 0 . DP=27;I16=13,13,0,0,986,38264,0,0,1529,90841,0,0,538,12532,0,0;QS=3,0;MQSB=0.945959;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,189:7:0 0,12,125:4:0 17 1929 . T 0 . DP=27;I16=13,13,0,0,990,38630,0,0,1529,90841,0,0,538,12562,0,0;QS=3,0;MQSB=0.945959;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,189:7:0 0,12,139:4:0 17 1930 . G 0 . DP=26;I16=13,11,0,0,905,34865,0,0,1409,83641,0,0,521,12271,0,0;QS=3,0;MQSB=0.931547;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,179:6:0 0,9,99:3:0 17 1931 . C 0 . DP=27;I16=15,12,0,0,967,36293,0,0,1546,91130,0,0,540,12578,0,0;QS=3,0;MQSB=0.99881;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,165:6:0 0,12,121:4:0 17 1932 . T 0 . DP=27;I16=14,13,0,0,998,38154,0,0,1546,91130,0,0,541,12605,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,15,143:5:0 0,15,146:5:0 17 1933 . T 0 . DP=27;I16=14,13,0,0,962,35344,0,0,1546,91130,0,0,542,12602,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,15,142:5:0 0,15,158:5:0 17 1934 . G 0 . DP=27;I16=13,13,0,0,987,38219,0,0,1529,90841,0,0,543,12569,0,0;QS=3,0;MQSB=0.945959;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,134:5:0 0,15,143:5:0 17 1935 . C 0 . DP=27;I16=14,12,0,0,963,36627,0,0,1529,90841,0,0,520,11930,0,0;QS=3,0;MQSB=0.937241;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,162:6:0 0,15,154:5:0 17 1936 . C 0 . DP=27;I16=14,13,0,0,1018,39406,0,0,1589,94441,0,0,547,12561,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,177:6:0 0,15,159:5:0 17 1937 . T 0 . DP=27;I16=14,13,0,0,1036,40636,0,0,1589,94441,0,0,547,12489,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,182:6:0 0,15,170:5:0 17 1938 . G 0 . DP=27;I16=14,13,0,0,1001,38377,0,0,1589,94441,0,0,546,12392,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,166:6:0 0,15,155:5:0 17 1939 . T 0 . DP=27;I16=14,12,0,0,964,36430,0,0,1560,93600,0,0,525,11909,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,167:6:0 0,12,144:4:0 17 1940 . G 0 . DP=27;I16=14,13,0,0,1003,37937,0,0,1589,94441,0,0,542,12172,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,159:6:0 0,15,155:5:0 17 1941 . G 0 . DP=27;I16=14,13,0,0,1004,38072,0,0,1589,94441,0,0,540,12098,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,176:6:0 0,15,156:5:0 17 1942 . C 0 . DP=27;I16=14,12,0,0,996,38790,0,0,1560,93600,0,0,516,11564,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,174:6:0 0,12,140:4:0 17 1943 . T 0 . DP=27;I16=14,13,0,0,1044,40952,0,0,1589,94441,0,0,536,12022,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,184:6:0 0,15,167:5:0 17 1944 . T 0 . DP=27;I16=14,13,0,0,987,37237,0,0,1589,94441,0,0,533,11971,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,175:6:0 0,15,170:5:0 17 1945 . T 0 . DP=27;I16=14,13,0,0,993,37067,0,0,1589,94441,0,0,530,11946,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,174:6:0 0,15,171:5:0 17 1946 . G 0 . DP=27;I16=14,13,0,0,1013,38617,0,0,1589,94441,0,0,526,11896,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,162:6:0 0,15,162:5:0 17 1947 . A 0 . DP=26;I16=13,13,0,0,950,35522,0,0,1529,90841,0,0,522,11818,0,0;QS=3,0;MQSB=0.945959;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,167:6:0 0,15,160:5:0 17 1948 . G C, 0 . DP=27;I16=14,12,0,1,966,36912,16,256,1560,93600,29,841,493,11135,25,625;QS=2.90361,0.0963855,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.94394;BQB=1;MQ0F=0 PL:DP:DV 0,45,255,45,255,255:15:0 0,21,190,21,190,190:7:0 1,0,123,13,126,132:5:1 17 1949 . A 0 . DP=26;I16=14,11,0,0,871,31325,0,0,1469,87241,0,0,490,11048,0,0;QS=3,0;MQSB=0.929204;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,174:7:0 0,15,155:5:0 17 1950 . A 0 . DP=27;I16=14,13,0,0,1007,38049,0,0,1589,94441,0,0,511,11559,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,21,205:7:0 0,18,176:6:0 17 1951 . G 0 . DP=27;I16=14,13,0,0,960,35536,0,0,1589,94441,0,0,509,11469,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,21,180:7:0 0,18,181:6:0 17 1952 . A 0 . DP=27;I16=14,12,0,0,924,33366,0,0,1529,90841,0,0,483,10779,0,0;QS=3,0;MQSB=0.937241;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,184:7:0 0,18,182:6:0 17 1953 . A 0 . DP=28;I16=15,12,0,0,925,32719,0,0,1589,94441,0,0,482,10740,0,0;QS=3,0;MQSB=0.935229;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,21,189:7:0 0,18,168:6:0 17 1954 . A C, 0 . DP=29;I16=14,13,0,1,929,33219,18,324,1620,97200,29,841,475,10679,25,625;QS=2.90217,0.0978261,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.949591;BQB=1;MQ0F=0 PL:DP:DV 0,45,255,45,255,255:15:0 0,21,198,21,198,198:7:0 0,0,130,15,133,141:6:1 17 1955 . C 0 . DP=29;I16=14,12,0,0,961,36067,0,0,1560,93600,0,0,451,10035,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,21,206:7:0 0,15,165:5:0 17 1956 . C 0 . DP=29;I16=14,13,0,0,964,35402,0,0,1620,97200,0,0,475,10573,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,196:7:0 0,15,164:5:0 17 1957 . C 0 . DP=29;I16=14,13,0,0,980,36212,0,0,1620,97200,0,0,472,10420,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,183:7:0 0,15,167:5:0 17 1958 . C 0 . DP=29;I16=15,13,0,0,1003,37293,0,0,1649,98041,0,0,493,10825,0,0;QS=3,0;MQSB=0.942064;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,191:7:0 0,18,190:6:0 17 1959 . T 0 . DP=29;I16=16,13,0,0,1112,43258,0,0,1709,101641,0,0,491,10593,0,0;QS=3,0;MQSB=0.940317;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,213:7:0 0,18,193:6:0 17 1960 . T 0 . DP=30;I16=17,13,0,0,1053,37939,0,0,1769,105241,0,0,485,10339,0,0;QS=3,0;MQSB=0.938685;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,196:7:0 0,18,186:6:0 17 1961 . C 0 . DP=30;I16=17,13,0,0,1101,41737,0,0,1769,105241,0,0,480,10122,0,0;QS=3,0;MQSB=0.938685;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,195:7:0 0,18,183:6:0 17 1962 . T 0 . DP=29;I16=17,12,0,0,1134,44582,0,0,1709,101641,0,0,477,9941,0,0;QS=3,0;MQSB=0.931617;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,208:7:0 0,18,198:6:0 17 1963 . G 0 . DP=28;I16=16,12,0,0,1066,41050,0,0,1649,98041,0,0,476,9794,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,192:7:0 0,18,188:6:0 17 1964 . G 0 . DP=28;I16=16,12,0,0,970,34764,0,0,1649,98041,0,0,475,9681,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,178:7:0 0,18,169:6:0 17 1965 . T 0 . DP=29;I16=16,12,0,0,964,34772,0,0,1649,98041,0,0,449,8977,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,196:7:0 0,18,171:6:0 17 1966 . T 0 . DP=29;I16=16,13,0,0,1029,37027,0,0,1709,101641,0,0,474,9558,0,0;QS=3,0;MQSB=0.940317;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,191:7:0 0,18,181:6:0 17 1967 . A 0 . DP=29;I16=16,13,0,0,1030,37234,0,0,1709,101641,0,0,474,9550,0,0;QS=3,0;MQSB=0.940317;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,187:7:0 0,18,182:6:0 17 1968 . T 0 . DP=29;I16=16,13,0,0,1075,40173,0,0,1709,101641,0,0,474,9578,0,0;QS=3,0;MQSB=0.940317;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,195:7:0 0,18,190:6:0 17 1969 . A 0 . DP=28;I16=16,12,0,0,992,35828,0,0,1649,98041,0,0,474,9592,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,175:6:0 0,18,186:6:0 17 1970 . C 0 . DP=28;I16=16,12,0,0,1015,37503,0,0,1649,98041,0,0,474,9642,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,170:6:0 0,18,180:6:0 17 1971 . A 0 . DP=29;I16=16,13,0,0,1073,40273,0,0,1709,101641,0,0,474,9728,0,0;QS=3,0;MQSB=0.940317;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,178:6:0 0,21,206:7:0 17 1972 . T 0 . DP=30;I16=16,14,0,0,1068,38978,0,0,1769,105241,0,0,475,9851,0,0;QS=3,0;MQSB=0.946202;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,176:6:0 0,21,203:7:0 17 1973 . A 0 . DP=30;I16=16,13,0,0,1046,38070,0,0,1740,104400,0,0,452,9388,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,172:6:0 0,18,196:6:0 17 1974 . A 0 . DP=29;I16=16,13,0,0,1086,41474,0,0,1709,101641,0,0,477,10065,0,0;QS=3,0;MQSB=0.940317;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,15,157:5:0 0,21,214:7:0 17 1975 . G 0 . DP=28;I16=16,12,0,0,1067,41171,0,0,1649,98041,0,0,478,10156,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,133:4:0 0,21,195:7:0 17 1976 . A 0 . DP=28;I16=16,12,0,0,981,34839,0,0,1649,98041,0,0,478,10234,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,125:4:0 0,21,199:7:0 17 1977 . C 0 . DP=28;I16=16,12,0,0,1009,37471,0,0,1649,98041,0,0,477,10297,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,132:4:0 0,21,194:7:0 17 1978 . A 0 . DP=28;I16=16,12,0,0,1082,42132,0,0,1649,98041,0,0,476,10394,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,144:4:0 0,21,220:7:0 17 1979 . G 0 . DP=28;I16=16,11,0,0,1019,38927,0,0,1589,94441,0,0,454,10064,0,0;QS=3,0;MQSB=0.925036;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,122:4:0 0,21,208:7:0 17 1980 . C 0 . DP=27;I16=16,10,0,0,932,34456,0,0,1529,90841,0,0,452,10114,0,0;QS=3,0;MQSB=0.914947;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,107:3:0 0,21,197:7:0 17 1981 . C 0 . DP=28;I16=16,12,0,0,998,36510,0,0,1649,98041,0,0,469,10479,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,9,101:3:0 0,24,219:8:0 17 1982 . A 0 . DP=29;I16=16,12,0,0,1035,38815,0,0,1649,98041,0,0,472,10548,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,110:3:0 0,27,254:9:0 17 1983 . G 0 . DP=28;I16=16,12,0,0,1048,40322,0,0,1649,98041,0,0,477,10599,0,0;QS=3,0;MQSB=0.933359;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,96:3:0 0,27,249:9:0 17 1984 . A 0 . DP=27;I16=16,11,0,0,1024,39232,0,0,1589,94441,0,0,482,10632,0,0;QS=3,0;MQSB=0.925036;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,70:2:0 0,27,255:9:0 17 1985 . G 0 . DP=27;I16=16,11,0,0,1009,38449,0,0,1589,94441,0,0,487,10695,0,0;QS=3,0;MQSB=0.925036;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,66:2:0 0,27,231:9:0 17 1986 . A 0 . DP=27;I16=16,10,0,0,926,33696,0,0,1560,93600,0,0,466,10112,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,76:2:0 0,24,223:8:0 17 1987 . A 0 . DP=28;I16=17,11,0,0,1034,39088,0,0,1649,98041,0,0,495,10807,0,0;QS=3,0;MQSB=0.923174;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,78:2:0 0,27,247:9:0 17 1988 . G 0 . DP=28;I16=17,11,0,0,1050,39980,0,0,1649,98041,0,0,499,10855,0,0;QS=3,0;MQSB=0.923174;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,63:2:0 0,27,239:9:0 17 1989 . G 0 . DP=28;I16=17,10,0,0,994,37610,0,0,1589,94441,0,0,493,10801,0,0;QS=3,0;MQSB=0.912952;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,73:2:0 0,27,237:9:0 17 1990 . G T, 0 . DP=28;I16=17,9,0,1,965,36359,33,1089,1560,93600,29,841,472,10250,25,625;QS=2.90675,0.0932476,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.912952;BQB=1;MQ0F=0 PL:DP:DV 0,48,255,48,255,255:16:0 0,6,71,6,71,71:2:0 2,0,195,26,198,215:9:1 17 1991 . A 0 . DP=28;I16=17,11,0,0,1017,38203,0,0,1649,98041,0,0,507,10975,0,0;QS=3,0;MQSB=0.923174;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,60:2:0 0,27,250:9:0 17 1992 . G 0 . DP=28;I16=17,11,0,0,1028,38852,0,0,1649,98041,0,0,509,11039,0,0;QS=3,0;MQSB=0.923174;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,62:2:0 0,27,231:9:0 17 1993 . T 0 . DP=28;I16=17,11,0,0,1015,37325,0,0,1649,98041,0,0,511,11131,0,0;QS=3,0;MQSB=0.923174;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,59:2:0 0,27,243:9:0 17 1994 . T 0 . DP=27;I16=16,11,0,0,942,33698,0,0,1589,94441,0,0,514,11250,0,0;QS=3,0;MQSB=0.925036;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,54:2:0 0,24,216:8:0 17 1995 . G 0 . DP=28;I16=16,11,0,0,960,35432,0,0,1620,97200,0,0,492,10770,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,66:2:0 0,21,188:7:0 17 1996 . C 0 . DP=29;I16=17,12,0,0,1022,37426,0,0,1709,101641,0,0,519,11469,0,0;QS=3,0;MQSB=0.931617;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,6,64:2:0 0,24,192:8:0 17 1997 . C 0 . DP=29;I16=17,11,0,0,934,32858,0,0,1649,98041,0,0,520,11524,0,0;QS=3,0;MQSB=0.923174;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,70:2:0 0,24,193:8:0 17 1998 . C 0 . DP=29;I16=17,12,0,0,1027,37843,0,0,1709,101641,0,0,522,11562,0,0;QS=3,0;MQSB=0.931617;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,6,69:2:0 0,24,222:8:0 17 1999 . A 0 . DP=28;I16=17,11,0,0,1073,41493,0,0,1649,98041,0,0,525,11627,0,0;QS=3,0;MQSB=0.923174;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,6,72:2:0 0,21,206:7:0 17 2000 . G 0 . DP=28;I16=16,11,0,0,1036,40576,0,0,1589,94441,0,0,511,11395,0,0;QS=3,0;MQSB=0.925036;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,6,72:2:0 0,18,168:6:0 17 2001 . G 0 . DP=28;I16=15,11,0,0,955,35661,0,0,1529,90841,0,0,489,10853,0,0;QS=3,0;MQSB=0.927041;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,62:2:0 0,18,172:6:0 17 2002 . G 0 . DP=28;I16=17,10,0,0,907,32227,0,0,1620,97200,0,0,519,11663,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,6,53:2:0 0,18,163:6:0 17 2003 . T 0 . DP=28;I16=17,11,0,0,920,31866,0,0,1649,98041,0,0,541,12163,0,0;QS=3,0;MQSB=0.923174;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,6,49:2:0 0,21,190:7:0 17 2004 . G 0 . DP=27;I16=16,10,0,0,970,36928,0,0,1560,93600,0,0,530,12110,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,57:2:0 0,18,178:6:0 17 2005 . G 0 . DP=27;I16=16,10,0,0,868,30936,0,0,1560,93600,0,0,536,12370,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,59:2:0 0,18,153:6:0 17 2006 . C 0 . DP=27;I16=16,10,0,0,968,37046,0,0,1560,93600,0,0,541,12605,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,73:2:0 0,18,166:6:0 17 2007 . A 0 . DP=27;I16=16,11,0,0,1000,37396,0,0,1589,94441,0,0,556,12882,0,0;QS=3,0;MQSB=0.925036;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,76:2:0 0,21,193:7:0 17 2008 . C 0 . DP=26;I16=16,10,0,0,964,36892,0,0,1529,90841,0,0,555,12833,0,0;QS=3,0;MQSB=0.914947;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,73:2:0 0,21,183:7:0 17 2009 . A 0 . DP=26;I16=16,10,0,0,997,38955,0,0,1529,90841,0,0,554,12802,0,0;QS=3,0;MQSB=0.914947;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,74:2:0 0,21,210:7:0 17 2010 . G 0 . DP=26;I16=16,9,0,0,936,36208,0,0,1500,90000,0,0,542,12640,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,73:2:0 0,18,169:6:0 17 2011 . C 0 . DP=26;I16=16,9,0,0,966,37960,0,0,1500,90000,0,0,541,12617,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,74:2:0 0,18,175:6:0 17 2012 . A 0 . DP=27;I16=16,11,0,0,956,35018,0,0,1589,94441,0,0,548,12676,0,0;QS=3,0;MQSB=0.925036;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,74:2:0 0,21,189:7:0 17 2013 . C 0 . DP=27;I16=15,10,0,0,876,31370,0,0,1500,90000,0,0,514,11950,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,69:2:0 0,18,160:6:0 17 2014 . G 0 . DP=27;I16=17,10,0,0,903,31239,0,0,1589,94441,0,0,545,12591,0,0;QS=3,0;MQSB=0.912952;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,9,80:3:0 0,18,160:6:0 17 2015 . T 0 . DP=27;I16=17,10,0,0,999,37507,0,0,1589,94441,0,0,545,12577,0,0;QS=3,0;MQSB=0.912952;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,9,95:3:0 0,18,178:6:0 17 2016 . T 0 . DP=27;I16=17,10,0,0,971,35649,0,0,1589,94441,0,0,545,12583,0,0;QS=3,0;MQSB=0.912952;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,9,93:3:0 0,18,178:6:0 17 2017 . G 0 . DP=28;I16=17,11,0,0,1013,37849,0,0,1649,98041,0,0,545,12609,0,0;QS=3,0;MQSB=0.923174;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,9,91:3:0 0,18,172:6:0 17 2018 . C 0 . DP=28;I16=17,11,0,0,1060,41414,0,0,1649,98041,0,0,546,12656,0,0;QS=3,0;MQSB=0.923174;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,9,97:3:0 0,18,169:6:0 17 2019 . T 0 . DP=28;I16=17,11,0,0,1083,42253,0,0,1649,98041,0,0,547,12725,0,0;QS=3,0;MQSB=0.923174;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,9,106:3:0 0,18,187:6:0 17 2020 . G 0 . DP=29;I16=18,11,0,0,1095,42063,0,0,1678,98882,0,0,548,12816,0,0;QS=3,0;MQSB=0.987702;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,9,85:3:0 0,18,175:6:0 17 2021 . C 0 . DP=29;I16=18,11,0,0,1081,41535,0,0,1709,101641,0,0,551,12877,0,0;QS=3,0;MQSB=0.969907;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,9,97:3:0 0,15,167:5:0 17 2022 . C 0 . DP=30;I16=19,11,0,0,1115,42003,0,0,1769,105241,0,0,555,12907,0,0;QS=3,0;MQSB=0.972375;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,9,92:3:0 0,18,177:6:0 17 2023 . A 0 . DP=30;I16=19,10,0,0,1063,39991,0,0,1709,101641,0,0,549,12837,0,0;QS=3,0;MQSB=0.974027;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,9,96:3:0 0,18,186:6:0 17 2024 . G 0 . DP=32;I16=20,12,0,0,1168,43872,0,0,1889,112441,0,0,564,12982,0,0;QS=3,0;MQSB=0.973096;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,9,92:3:0 0,24,203:8:0 17 2025 . T 0 . DP=32;I16=20,12,0,0,1150,42122,0,0,1889,112441,0,0,569,12981,0,0;QS=3,0;MQSB=0.973096;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,9,87:3:0 0,24,226:8:0 17 2026 . T 0 . DP=32;I16=20,12,0,0,1130,40724,0,0,1889,112441,0,0,572,12908,0,0;QS=3,0;MQSB=0.973096;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,9,94:3:0 0,24,208:8:0 17 2027 . A 0 . DP=32;I16=19,12,0,0,1121,40871,0,0,1829,108841,0,0,550,12240,0,0;QS=3,0;MQSB=0.970829;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,9,84:3:0 0,21,207:7:0 17 2028 . C 0 . DP=32;I16=20,12,0,0,1242,48548,0,0,1889,112441,0,0,577,12803,0,0;QS=3,0;MQSB=0.973096;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,9,94:3:0 0,24,228:8:0 17 2029 . T 0 . DP=32;I16=20,12,0,0,1229,47605,0,0,1889,112441,0,0,578,12724,0,0;QS=3,0;MQSB=0.973096;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,9,102:3:0 0,24,236:8:0 17 2030 . G 0 . DP=32;I16=20,12,0,0,1222,47140,0,0,1889,112441,0,0,579,12679,0,0;QS=3,0;MQSB=0.973096;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,9,93:3:0 0,24,216:8:0 17 2031 . C 0 . DP=31;I16=19,12,0,0,1150,43656,0,0,1829,108841,0,0,581,12667,0,0;QS=3,0;MQSB=0.970829;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,9,96:3:0 0,24,204:8:0 17 2032 . C 0 . DP=31;I16=19,12,0,0,1157,44035,0,0,1829,108841,0,0,583,12687,0,0;QS=3,0;MQSB=0.970829;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,9,90:3:0 0,24,210:8:0 17 2033 . A 0 . DP=30;I16=19,11,0,0,1091,40223,0,0,1769,105241,0,0,585,12689,0,0;QS=3,0;MQSB=0.972375;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,9,93:3:0 0,21,203:7:0 17 2034 . T 0 . DP=30;I16=19,11,0,0,1104,41498,0,0,1769,105241,0,0,587,12723,0,0;QS=3,0;MQSB=0.972375;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,9,91:3:0 0,21,208:7:0 17 2035 . T 0 . DP=29;I16=18,11,0,0,1084,41024,0,0,1709,101641,0,0,589,12739,0,0;QS=3,0;MQSB=0.969907;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,6,69:2:0 0,21,211:7:0 17 2036 . T 0 . DP=29;I16=18,11,0,0,1080,40612,0,0,1709,101641,0,0,591,12787,0,0;QS=3,0;MQSB=0.969907;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,6,67:2:0 0,21,216:7:0 17 2037 . T 0 . DP=29;I16=18,11,0,0,1082,40956,0,0,1709,101641,0,0,592,12818,0,0;QS=3,0;MQSB=0.969907;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,6,69:2:0 0,21,217:7:0 17 2038 . C 0 . DP=29;I16=18,11,0,0,1117,43573,0,0,1709,101641,0,0,592,12832,0,0;QS=3,0;MQSB=0.969907;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,6,69:2:0 0,21,211:7:0 17 2039 . A 0 . DP=29;I16=18,11,0,0,1067,39581,0,0,1709,101641,0,0,592,12878,0,0;QS=3,0;MQSB=0.969907;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,6,72:2:0 0,21,212:7:0 17 2040 . C 0 . DP=29;I16=18,11,0,0,1077,40469,0,0,1709,101641,0,0,590,12856,0,0;QS=3,0;MQSB=0.969907;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,6,69:2:0 0,21,196:7:0 17 2041 . G A, 0 . DP=31;I16=6,5,12,7,389,14023,721,28149,660,39600,1109,65641,235,5607,353,7259;QS=0.917304,2.0827,0;VDB=0.816435;SGB=-4.18892;RPB=0.88473;MQB=0.972375;MQSB=0.968257;BQB=0.311275;MQ0F=0 PL:DP:DV 229,0,212,255,245,255:21:11 32,0,24,35,27,59:2:1 223,21,0,223,21,223:7:7 17 2042 . G 0 . DP=32;I16=18,14,0,0,1189,45617,0,0,1889,112441,0,0,588,12910,0,0;QS=3,0;MQSB=0.965264;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,9,86:3:0 0,21,206:7:0 17 2043 . G 0 . DP=32;I16=17,14,0,0,1115,41585,0,0,1829,108841,0,0,581,12891,0,0;QS=3,0;MQSB=0.962133;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,9,90:3:0 0,21,197:7:0 17 2044 . C 0 . DP=32;I16=18,14,0,0,1213,47029,0,0,1889,112441,0,0,588,13006,0,0;QS=3,0;MQSB=0.965264;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,9,85:3:0 0,21,207:7:0 17 2045 . A 0 . DP=32;I16=18,14,0,0,1181,44527,0,0,1889,112441,0,0,588,13108,0,0;QS=3,0;MQSB=0.965264;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,9,83:3:0 0,21,213:7:0 17 2046 . T 0 . DP=32;I16=18,14,0,0,1197,45135,0,0,1889,112441,0,0,587,13195,0,0;QS=3,0;MQSB=0.965264;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,9,103:3:0 0,21,216:7:0 17 2047 . G 0 . DP=34;I16=17,16,0,0,1248,47798,0,0,1949,116041,0,0,557,12491,0,0;QS=3,0;MQSB=0.959328;MQ0F=0 PL:DP:DV 0,72,255:24:0 0,9,88:3:0 0,18,195:6:0 17 2048 . A 0 . DP=34;I16=18,16,0,0,1230,45184,0,0,2009,119641,0,0,578,13022,0,0;QS=3,0;MQSB=0.962621;MQ0F=0 PL:DP:DV 0,72,255:24:0 0,9,87:3:0 0,21,206:7:0 17 2049 . A 0 . DP=33;I16=17,16,0,0,1207,44567,0,0,1949,116041,0,0,575,12963,0,0;QS=3,0;MQSB=0.959328;MQ0F=0 PL:DP:DV 0,72,255:24:0 0,6,74:2:0 0,21,208:7:0 17 2050 . A 0 . DP=33;I16=16,16,0,0,1169,43313,0,0,1889,112441,0,0,545,12211,0,0;QS=3,0;MQSB=0.955563;MQ0F=0 PL:DP:DV 0,72,255:24:0 0,6,77:2:0 0,18,188:6:0 17 2051 . T 0 . DP=31;I16=16,15,0,0,1134,42164,0,0,1829,108841,0,0,567,12737,0,0;QS=3,0;MQSB=0.957006;MQ0F=0 PL:DP:DV 0,69,255:23:0 0,6,69:2:0 0,18,170:6:0 17 2052 . G 0 . DP=31;I16=16,15,0,0,1180,45546,0,0,1829,108841,0,0,564,12664,0,0;QS=3,0;MQSB=0.957006;MQ0F=0 PL:DP:DV 0,69,255:23:0 0,6,74:2:0 0,18,175:6:0 17 2053 . G T, 0 . DP=31;I16=15,15,0,1,1126,42672,23,529,1769,105241,60,3600,537,11991,25,625;QS=2.97307,0.0269321,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.951229;BQB=1;MQ0F=0 PL:DP:DV 0,46,255,66,255,255:23:1 0,6,71,6,71,71:2:0 0,18,180,18,180,180:6:0 17 2054 . A 0 . DP=30;I16=15,15,0,0,1123,43027,0,0,1769,105241,0,0,562,12592,0,0;QS=3,0;MQSB=0.952765;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,6,78:2:0 0,18,171:6:0 17 2055 . G 0 . DP=30;I16=15,15,0,0,1156,45206,0,0,1769,105241,0,0,562,12592,0,0;QS=3,0;MQSB=0.952765;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,6,66:2:0 0,18,168:6:0 17 2056 . A 0 . DP=30;I16=15,15,0,0,1124,42416,0,0,1769,105241,0,0,560,12518,0,0;QS=3,0;MQSB=0.952765;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,6,71:2:0 0,18,179:6:0 17 2057 . T 0 . DP=30;I16=15,15,0,0,1094,40082,0,0,1769,105241,0,0,556,12374,0,0;QS=3,0;MQSB=0.952765;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,6,72:2:0 0,18,178:6:0 17 2058 . A 0 . DP=29;I16=14,15,0,0,1035,37517,0,0,1709,101641,0,0,552,12212,0,0;QS=3,0;MQSB=0.947838;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,6,57:2:0 0,18,179:6:0 17 2059 . A 0 . DP=29;I16=14,15,0,0,1063,39615,0,0,1709,101641,0,0,548,12082,0,0;QS=3,0;MQSB=0.947838;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,6,66:2:0 0,18,188:6:0 17 2060 . C 0 . DP=28;I16=12,15,0,0,1015,39057,0,0,1589,94441,0,0,523,11499,0,0;QS=3,0;MQSB=0.935229;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,6,62:2:0 0,15,143:5:0 17 2061 . A 0 . DP=27;I16=12,14,0,0,950,35084,0,0,1529,90841,0,0,501,11073,0,0;QS=3,0;MQSB=0.937241;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,3,41:1:0 0,15,163:5:0 17 2062 . A 0 . DP=26;I16=11,15,0,0,973,37349,0,0,1529,90841,0,0,519,11425,0,0;QS=3,0;MQSB=0.927041;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,6,57:2:0 0,15,164:5:0 17 2063 . C 0 . DP=26;I16=11,15,0,0,1017,40149,0,0,1529,90841,0,0,517,11405,0,0;QS=3,0;MQSB=0.927041;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,6,74:2:0 0,15,158:5:0 17 2064 . A 0 . DP=26;I16=11,15,0,0,1002,38980,0,0,1529,90841,0,0,515,11413,0,0;QS=3,0;MQSB=0.927041;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,6,75:2:0 0,15,164:5:0 17 2065 . G 0 . DP=26;I16=12,14,0,0,1030,41232,0,0,1529,90841,0,0,514,11448,0,0;QS=3,0;MQSB=0.937241;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,78:2:0 0,18,167:6:0 17 2066 . G 0 . DP=26;I16=12,14,0,0,973,37055,0,0,1529,90841,0,0,514,11510,0,0;QS=3,0;MQSB=0.937241;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,75:2:0 0,18,152:6:0 17 2067 . A 0 . DP=26;I16=12,14,0,0,1005,39095,0,0,1529,90841,0,0,512,11498,0,0;QS=3,0;MQSB=0.937241;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,69:2:0 0,18,187:6:0 17 2068 . G 0 . DP=26;I16=12,14,0,0,993,39005,0,0,1529,90841,0,0,509,11459,0,0;QS=3,0;MQSB=0.937241;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,53:2:0 0,18,167:6:0 17 2069 . C 0 . DP=26;I16=12,14,0,0,873,29879,0,0,1529,90841,0,0,506,11442,0,0;QS=3,0;MQSB=0.937241;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,59:2:0 0,18,156:6:0 17 2070 . G 0 . DP=27;I16=13,14,0,0,932,33090,0,0,1589,94441,0,0,502,11398,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,6,62:2:0 0,18,145:6:0 17 2071 . A 0 . DP=27;I16=13,14,0,0,946,33884,0,0,1589,94441,0,0,498,11330,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,6,53:2:0 0,18,177:6:0 17 2072 . C 0 . DP=25;I16=13,12,0,0,897,32897,0,0,1469,87241,0,0,496,11288,0,0;QS=3,0;MQSB=0.948139;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,59:2:0 0,15,128:5:0 17 2073 . C 0 . DP=26;I16=14,12,0,0,874,30184,0,0,1529,90841,0,0,492,11168,0,0;QS=3,0;MQSB=0.953497;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,6,53:2:0 0,15,133:5:0 17 2074 . G 0 . DP=26;I16=13,12,0,0,835,29219,0,0,1469,87241,0,0,463,10395,0,0;QS=3,0;MQSB=0.948139;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,6,67:2:0 0,12,83:4:0 17 2075 . C 0 . DP=27;I16=15,12,0,0,989,37027,0,0,1589,94441,0,0,484,10896,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,6,69:2:0 0,15,115:5:0 17 2076 . A 0 . DP=27;I16=14,12,0,0,929,33551,0,0,1529,90841,0,0,470,10676,0,0;QS=3,0;MQSB=0.953497;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,6,76:2:0 0,12,114:4:0 17 2077 . C 0 . DP=27;I16=15,12,0,0,990,37252,0,0,1589,94441,0,0,478,10724,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,6,65:2:0 0,15,125:5:0 17 2078 . A 0 . DP=27;I16=15,12,0,0,1023,39089,0,0,1589,94441,0,0,475,10677,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,6,72:2:0 0,15,148:5:0 17 2079 . G 0 . DP=28;I16=15,13,0,0,1042,39694,0,0,1649,98041,0,0,471,10605,0,0;QS=3,0;MQSB=0.956162;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,6,72:2:0 0,15,133:5:0 17 2080 . G 0 . DP=28;I16=14,13,0,0,968,35328,0,0,1589,94441,0,0,453,10333,0,0;QS=3,0;MQSB=0.951472;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,6,67:2:0 0,12,108:4:0 17 2081 . C 0 . DP=26;I16=14,12,0,0,937,35303,0,0,1529,90841,0,0,467,10535,0,0;QS=3,0;MQSB=0.953497;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,6,63:2:0 0,15,110:5:0 17 2082 . T 0 . DP=24;I16=12,12,0,0,901,34287,0,0,1409,83641,0,0,468,10532,0,0;QS=3,0;MQSB=0.941765;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,71:2:0 0,12,112:4:0 17 2083 . G 0 . DP=24;I16=12,12,0,0,887,33597,0,0,1409,83641,0,0,469,10547,0,0;QS=3,0;MQSB=0.941765;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,68:2:0 0,12,113:4:0 17 2084 . C 0 . DP=25;I16=13,12,0,0,938,35868,0,0,1469,87241,0,0,470,10580,0,0;QS=3,0;MQSB=0.948139;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,6,61:2:0 0,12,115:4:0 17 2085 . T 0 . DP=25;I16=13,12,0,0,932,35282,0,0,1469,87241,0,0,472,10632,0,0;QS=3,0;MQSB=0.948139;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,6,64:2:0 0,12,130:4:0 17 2086 . G 0 . DP=25;I16=13,12,0,0,932,35400,0,0,1469,87241,0,0,474,10704,0,0;QS=3,0;MQSB=0.948139;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,6,61:2:0 0,12,129:4:0 17 2087 . A 0 . DP=24;I16=12,12,0,0,903,34391,0,0,1409,83641,0,0,476,10746,0,0;QS=3,0;MQSB=0.941765;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,66:2:0 0,12,126:4:0 17 2088 . G 0 . DP=24;I16=12,12,0,0,880,33116,0,0,1409,83641,0,0,478,10808,0,0;QS=3,0;MQSB=0.941765;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,70:2:0 0,12,121:4:0 17 2089 . C 0 . DP=25;I16=13,12,0,0,817,27419,0,0,1469,87241,0,0,480,10890,0,0;QS=3,0;MQSB=0.948139;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,64:2:0 0,15,138:5:0 17 2090 . G 0 . DP=25;I16=12,12,0,0,802,27940,0,0,1409,83641,0,0,457,10319,0,0;QS=3,0;MQSB=0.941765;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,63:2:0 0,12,99:4:0 17 2091 . C 0 . DP=25;I16=12,12,0,0,800,27346,0,0,1440,86400,0,0,458,10346,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,61:2:0 0,15,125:5:0 17 2092 . G 0 . DP=26;I16=13,12,0,0,838,29188,0,0,1469,87241,0,0,461,10487,0,0;QS=3,0;MQSB=0.948139;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,64:2:0 0,18,133:6:0 17 2093 . T G, 0 . DP=26;I16=13,12,1,0,905,33415,17,289,1500,90000,29,841,459,10371,25,625;QS=2.97424,0.0257576,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.953497;BQB=1;MQ0F=0 PL:DP:DV 0,36,255,51,255,255:18:1 0,6,67,6,67,67:2:0 0,18,153,18,153,153:6:0 17 2094 . C 0 . DP=26;I16=14,12,0,0,949,35501,0,0,1529,90841,0,0,485,11047,0,0;QS=3,0;MQSB=0.953497;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,69:2:0 0,18,142:6:0 17 2095 . A 0 . DP=25;I16=14,11,0,0,879,31219,0,0,1469,87241,0,0,487,11123,0,0;QS=3,0;MQSB=0.955682;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,72:2:0 0,18,154:6:0 17 2096 . C 0 . DP=24;I16=13,11,0,0,878,32734,0,0,1409,83641,0,0,487,11073,0,0;QS=3,0;MQSB=0.950498;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,65:2:0 0,18,161:6:0 17 2097 . A 0 . DP=24;I16=13,11,0,0,849,30671,0,0,1409,83641,0,0,487,11047,0,0;QS=3,0;MQSB=0.950498;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,56:2:0 0,18,172:6:0 17 2098 . C 0 . DP=24;I16=13,11,0,0,781,26113,0,0,1409,83641,0,0,486,10996,0,0;QS=3,0;MQSB=0.950498;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,58:2:0 0,18,139:6:0 17 2099 . G 0 . DP=23;I16=12,10,0,0,719,25109,0,0,1289,76441,0,0,460,10294,0,0;QS=3,0;MQSB=0.947103;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,3,24:1:0 0,18,129:6:0 17 2100 . C 0 . DP=25;I16=13,11,0,0,873,32759,0,0,1409,83641,0,0,457,10141,0,0;QS=3,0;MQSB=0.950498;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,87:3:0 0,18,160:6:0 17 2101 . A 0 . DP=25;I16=12,12,0,0,907,34671,0,0,1440,86400,0,0,455,9965,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,135:4:0 0,18,161:6:0 17 2102 . G 0 . DP=25;I16=13,12,0,0,930,35596,0,0,1469,87241,0,0,478,10442,0,0;QS=3,0;MQSB=0.948139;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,118:4:0 0,18,151:6:0 17 2103 . C 0 . DP=25;I16=11,12,0,0,842,31630,0,0,1380,82800,0,0,432,9336,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,106:4:0 0,15,146:5:0 17 2104 . C 0 . DP=24;I16=12,12,0,0,869,32463,0,0,1409,83641,0,0,454,9810,0,0;QS=3,0;MQSB=0.941765;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,126:4:0 0,15,147:5:0 17 2105 . A 0 . DP=25;I16=11,12,0,0,836,30696,0,0,1380,82800,0,0,429,9201,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,138:4:0 0,15,130:5:0 17 2106 . T 0 . DP=25;I16=12,13,0,0,897,33087,0,0,1469,87241,0,0,473,10211,0,0;QS=3,0;MQSB=0.939413;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,129:4:0 0,15,146:5:0 17 2107 . C 0 . DP=25;I16=11,12,0,0,783,27185,0,0,1380,82800,0,0,425,9113,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,100:3:0 0,15,133:5:0 17 2108 . G 0 . DP=25;I16=11,13,0,0,829,29703,0,0,1440,86400,0,0,448,9730,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,122:4:0 0,15,111:5:0 17 2109 . C 0 . DP=25;I16=11,13,0,0,781,26717,0,0,1440,86400,0,0,446,9746,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,109:4:0 0,15,125:5:0 17 2110 . G 0 . DP=25;I16=12,12,0,0,804,28134,0,0,1440,86400,0,0,418,9110,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,92:3:0 0,15,117:5:0 17 2111 . C 0 . DP=25;I16=12,13,0,0,925,35081,0,0,1500,90000,0,0,441,9747,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,134:4:0 0,15,140:5:0 17 2112 . A 0 . DP=24;I16=12,12,0,0,885,33445,0,0,1440,86400,0,0,440,9782,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,129:4:0 0,15,140:5:0 17 2113 . G 0 . DP=24;I16=12,12,0,0,871,32641,0,0,1440,86400,0,0,439,9839,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,128:4:0 0,15,134:5:0 17 2114 . C 0 . DP=24;I16=12,11,0,0,844,32052,0,0,1380,82800,0,0,413,9293,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,103:3:0 0,15,126:5:0 17 2115 . T 0 . DP=23;I16=11,11,0,0,821,30869,0,0,1320,79200,0,0,412,9342,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,78:2:0 0,15,143:5:0 17 2116 . C 0 . DP=23;I16=11,12,0,0,890,34892,0,0,1380,82800,0,0,435,9985,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,102:3:0 0,15,152:5:0 17 2117 . A 0 . DP=23;I16=11,11,0,0,833,32121,0,0,1320,79200,0,0,432,9924,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,118:3:0 0,15,156:5:0 17 2118 . G 0 . DP=23;I16=12,11,0,0,842,31502,0,0,1380,82800,0,0,429,9835,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,125:4:0 0,15,147:5:0 17 2119 . G 0 . DP=23;I16=12,11,0,0,823,30553,0,0,1380,82800,0,0,426,9768,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,135:4:0 0,15,137:5:0 17 2120 . G 0 . DP=24;I16=13,11,0,0,864,32028,0,0,1440,86400,0,0,423,9723,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,137:4:0 0,15,140:5:0 17 2121 . A 0 . DP=22;I16=11,10,0,0,734,26712,0,0,1260,75600,0,0,398,9074,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,135:4:0 0,12,122:4:0 17 2122 . T 0 . DP=22;I16=11,10,0,0,752,27278,0,0,1260,75600,0,0,396,8972,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,136:4:0 0,12,123:4:0 17 2123 . A 0 . DP=22;I16=12,10,0,0,758,27024,0,0,1320,79200,0,0,419,9519,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,120:4:0 0,15,137:5:0 17 2124 . T 0 . DP=22;I16=12,10,0,0,789,28741,0,0,1320,79200,0,0,417,9465,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,128:4:0 0,15,136:5:0 17 2125 . T 0 . DP=20;I16=11,8,0,0,696,25704,0,0,1140,68400,0,0,401,9177,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,106:3:0 0,9,93:3:0 17 2126 . A 0 . DP=20;I16=11,9,0,0,717,25979,0,0,1200,72000,0,0,415,9319,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,133:4:0 0,9,95:3:0 17 2127 . C 0 . DP=21;I16=11,10,0,0,706,24426,0,0,1260,75600,0,0,413,9221,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,107:4:0 0,9,95:3:0 17 2128 . G 0 . DP=21;I16=11,10,0,0,677,22633,0,0,1260,75600,0,0,411,9091,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,116:4:0 0,9,72:3:0 17 2129 . T 0 . DP=21;I16=11,10,0,0,782,29386,0,0,1260,75600,0,0,409,8981,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,140:4:0 0,9,83:3:0 17 2130 . G 0 . DP=21;I16=11,10,0,0,766,28562,0,0,1260,75600,0,0,407,8891,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,135:4:0 0,9,87:3:0 17 2131 . T 0 . DP=21;I16=11,10,0,0,732,26216,0,0,1260,75600,0,0,405,8821,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,136:4:0 0,9,79:3:0 17 2132 . A 0 . DP=21;I16=11,10,0,0,743,26733,0,0,1260,75600,0,0,403,8771,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,135:4:0 0,9,88:3:0 17 2133 . A 0 . DP=21;I16=11,10,0,0,787,29651,0,0,1260,75600,0,0,401,8741,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,141:4:0 0,9,96:3:0 17 2134 . C 0 . DP=21;I16=11,10,0,0,804,31100,0,0,1260,75600,0,0,399,8731,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,138:4:0 0,9,86:3:0 17 2135 . T 0 . DP=22;I16=11,11,0,0,831,32197,0,0,1320,79200,0,0,397,8741,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,153:4:0 0,12,119:4:0 17 2136 . C 0 . DP=22;I16=11,11,0,0,760,26892,0,0,1320,79200,0,0,395,8721,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,124:4:0 0,12,127:4:0 17 2137 . G 0 . DP=22;I16=11,11,0,0,745,26047,0,0,1320,79200,0,0,393,8721,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,121:4:0 0,12,102:4:0 17 2138 . A 0 . DP=22;I16=11,11,0,0,816,30934,0,0,1320,79200,0,0,391,8741,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,128:4:0 0,12,139:4:0 17 2139 . C 0 . DP=22;I16=11,11,0,0,839,32237,0,0,1320,79200,0,0,389,8781,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,138:4:0 0,12,125:4:0 17 2140 . A 0 . DP=22;I16=11,11,0,0,826,31300,0,0,1320,79200,0,0,387,8841,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,140:4:0 0,12,138:4:0 17 2141 . T 0 . DP=21;I16=11,10,0,0,792,30156,0,0,1260,75600,0,0,385,8871,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,137:4:0 0,12,137:4:0 17 2142 . G 0 . DP=19;I16=11,8,0,0,724,27784,0,0,1140,68400,0,0,385,8919,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,113:3:0 0,12,129:4:0 17 2143 . T 0 . DP=19;I16=11,8,0,0,650,23454,0,0,1140,68400,0,0,384,8932,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,113:3:0 0,12,120:4:0 17 2144 . C 0 . DP=19;I16=11,8,0,0,739,29003,0,0,1140,68400,0,0,383,8959,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,108:3:0 0,12,125:4:0 17 2145 . A 0 . DP=20;I16=12,8,0,0,760,29304,0,0,1200,72000,0,0,381,8951,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,133:4:0 0,12,139:4:0 17 2146 . G 0 . DP=20;I16=12,8,0,0,745,28105,0,0,1200,72000,0,0,379,8909,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,139:4:0 0,12,127:4:0 17 2147 . C 0 . DP=20;I16=13,6,0,0,674,24296,0,0,1140,68400,0,0,379,8881,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,127:4:0 0,12,130:4:0 17 2148 . G 0 . DP=20;I16=12,7,0,0,630,21274,0,0,1140,68400,0,0,365,8537,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,250:11:0 0,12,112:4:0 0,12,120:4:0 17 2149 . A 0 . DP=20;I16=12,7,0,0,702,26212,0,0,1140,68400,0,0,367,8529,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,138:4:0 0,12,127:4:0 17 2150 . T 0 . DP=20;I16=13,7,0,0,675,23943,0,0,1200,72000,0,0,383,8713,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,247:11:0 0,12,138:4:0 0,15,139:5:0 17 2151 . T 0 . DP=20;I16=13,7,0,0,690,24274,0,0,1200,72000,0,0,383,8661,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,251:11:0 0,12,133:4:0 0,15,142:5:0 17 2152 . G 0 . DP=20;I16=13,7,0,0,693,24713,0,0,1200,72000,0,0,383,8629,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,131:4:0 0,15,146:5:0 17 2153 . T 0 . DP=19;I16=13,6,0,0,671,24571,0,0,1140,68400,0,0,383,8565,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,230:10:0 0,12,134:4:0 0,15,162:5:0 17 2154 . C 0 . DP=19;I16=13,6,0,0,703,26955,0,0,1140,68400,0,0,382,8468,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,251:10:0 0,12,131:4:0 0,15,159:5:0 17 2155 . A 0 . DP=19;I16=13,6,0,0,693,25727,0,0,1140,68400,0,0,381,8389,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,245:10:0 0,12,135:4:0 0,15,156:5:0 17 2156 . C 0 . DP=19;I16=13,6,0,0,702,26214,0,0,1140,68400,0,0,380,8328,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,125:4:0 0,15,154:5:0 17 2157 . A 0 . DP=19;I16=13,6,0,0,722,28058,0,0,1140,68400,0,0,379,8285,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,250:10:0 0,12,141:4:0 0,15,173:5:0 17 2158 . G 0 . DP=19;I16=13,6,0,0,713,27567,0,0,1140,68400,0,0,378,8260,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,136:4:0 0,15,144:5:0 17 2159 . G 0 . DP=19;I16=12,6,0,0,662,24744,0,0,1080,64800,0,0,366,8104,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,247:9:0 0,12,126:4:0 0,15,153:5:0 17 2160 . C 0 . DP=19;I16=12,6,0,0,651,24005,0,0,1080,64800,0,0,364,8038,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,244:9:0 0,12,119:4:0 0,15,154:5:0 17 2161 . A 0 . DP=19;I16=13,6,0,0,695,25883,0,0,1140,68400,0,0,369,8005,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,248:10:0 0,12,121:4:0 0,15,164:5:0 17 2162 . C 0 . DP=20;I16=13,7,0,0,742,27834,0,0,1200,72000,0,0,365,7911,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,127:4:0 0,15,155:5:0 17 2163 . T 0 . DP=20;I16=13,7,0,0,763,29517,0,0,1200,72000,0,0,362,7838,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,131:4:0 0,15,175:5:0 17 2164 . G 0 . DP=20;I16=13,7,0,0,715,26301,0,0,1200,72000,0,0,359,7787,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,116:4:0 0,15,156:5:0 17 2165 . C 0 . DP=20;I16=13,7,0,0,753,28781,0,0,1200,72000,0,0,355,7709,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,136:4:0 0,15,151:5:0 17 2166 . T 0 . DP=19;I16=12,7,0,0,711,27211,0,0,1140,68400,0,0,352,7654,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,142:4:0 0,12,140:4:0 17 2167 . A 0 . DP=19;I16=12,6,0,0,650,23760,0,0,1080,64800,0,0,327,7137,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,254:11:0 0,12,133:4:0 0,9,111:3:0 17 2168 . C 0 . DP=19;I16=12,7,0,0,685,25039,0,0,1140,68400,0,0,345,7561,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,130:4:0 0,12,124:4:0 17 2169 . T 0 . DP=19;I16=12,7,0,0,702,26940,0,0,1140,68400,0,0,341,7525,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,140:4:0 0,12,142:4:0 17 2170 . C 0 . DP=18;I16=11,7,0,0,665,24861,0,0,1080,64800,0,0,338,7512,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,132:4:0 0,12,135:4:0 17 2171 . C 0 . DP=20;I16=11,9,0,0,750,28368,0,0,1200,72000,0,0,333,7419,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,136:4:0 0,12,138:4:0 17 2172 . T 0 . DP=20;I16=11,9,0,0,732,27540,0,0,1200,72000,0,0,330,7346,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,149:4:0 0,12,147:4:0 17 2173 . G 0 . DP=19;I16=10,9,0,0,704,26534,0,0,1140,68400,0,0,327,7243,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,142:4:0 0,12,133:4:0 17 2174 . G 0 . DP=19;I16=10,9,0,0,674,24372,0,0,1140,68400,0,0,324,7158,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,126:4:0 0,12,129:4:0 17 2175 . G 0 . DP=18;I16=9,9,0,0,639,23059,0,0,1080,64800,0,0,322,7090,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,123:4:0 0,12,126:4:0 17 2176 . G 0 . DP=18;I16=9,9,0,0,621,21815,0,0,1080,64800,0,0,318,6940,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,247:10:0 0,12,121:4:0 0,12,123:4:0 17 2177 . T 0 . DP=18;I16=9,9,0,0,547,18051,0,0,1080,64800,0,0,314,6810,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,239:10:0 0,12,83:4:0 0,12,108:4:0 17 2178 . T 0 . DP=18;I16=9,9,0,0,579,19659,0,0,1080,64800,0,0,310,6700,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,234:10:0 0,12,110:4:0 0,12,118:4:0 17 2179 . T 0 . DP=18;I16=9,9,0,0,591,20403,0,0,1080,64800,0,0,307,6609,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,239:10:0 0,12,97:4:0 0,12,135:4:0 17 2180 . T 0 . DP=18;I16=9,9,0,0,616,21524,0,0,1080,64800,0,0,305,6537,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,236:10:0 0,12,122:4:0 0,12,133:4:0 17 2181 . C 0 . DP=18;I16=9,8,0,0,611,22485,0,0,1020,61200,0,0,293,6385,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,224:9:0 0,12,129:4:0 0,12,150:4:0 17 2182 . C 0 . DP=18;I16=9,9,0,0,665,24877,0,0,1080,64800,0,0,301,6453,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,125:4:0 0,12,145:4:0 17 2183 . A 0 . DP=18;I16=9,9,0,0,646,23624,0,0,1080,64800,0,0,299,6441,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,247:10:0 0,12,123:4:0 0,12,144:4:0 17 2184 . T 0 . DP=17;I16=8,9,0,0,610,22250,0,0,1020,61200,0,0,298,6448,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,224:9:0 0,12,134:4:0 0,12,136:4:0 17 2185 . C 0 . DP=16;I16=8,8,0,0,569,20761,0,0,960,57600,0,0,297,6423,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,211:8:0 0,12,128:4:0 0,12,137:4:0 17 2186 . A 0 . DP=16;I16=8,8,0,0,576,21314,0,0,960,57600,0,0,296,6416,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,204:8:0 0,12,134:4:0 0,12,145:4:0 17 2187 . A 0 . DP=16;I16=8,8,0,0,562,20396,0,0,960,57600,0,0,295,6427,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,201:8:0 0,12,136:4:0 0,12,135:4:0 17 2188 . A 0 . DP=17;I16=9,8,0,0,569,19925,0,0,1020,61200,0,0,293,6405,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,214:9:0 0,12,133:4:0 0,12,123:4:0 17 2189 . C 0 . DP=19;I16=9,10,0,0,645,22647,0,0,1097,65089,0,0,292,6400,0,0;QS=3,0;MQSB=0.934728;MQ0F=0 PL:DP:DV 0,30,230:10:0 0,12,133:4:0 0,15,142:5:0 17 2190 . C 0 . DP=19;I16=9,10,0,0,604,20072,0,0,1097,65089,0,0,294,6414,0,0;QS=3,0;MQSB=0.934728;MQ0F=0 PL:DP:DV 0,33,238:11:0 0,12,119:4:0 0,12,107:4:0 17 2191 . C 0 . DP=19;I16=9,10,0,0,647,23007,0,0,1097,65089,0,0,297,6449,0,0;QS=3,0;MQSB=0.934728;MQ0F=0 PL:DP:DV 0,33,251:11:0 0,12,129:4:0 0,12,119:4:0 17 2192 . T 0 . DP=19;I16=9,10,0,0,659,23723,0,0,1097,65089,0,0,300,6506,0,0;QS=3,0;MQSB=0.934728;MQ0F=0 PL:DP:DV 0,33,253:11:0 0,12,136:4:0 0,12,120:4:0 17 2193 . C 0 . DP=18;I16=8,10,0,0,642,23934,0,0,1037,61489,0,0,303,6535,0,0;QS=3,0;MQSB=0.938795;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,128:4:0 0,9,87:3:0 17 2194 . A 0 . DP=18;I16=8,9,0,0,617,22683,0,0,1020,61200,0,0,301,6561,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,247:10:0 0,12,134:4:0 0,9,98:3:0 17 2195 . A 0 . DP=18;I16=8,10,0,0,635,23271,0,0,1037,61489,0,0,309,6659,0,0;QS=3,0;MQSB=0.938795;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,128:4:0 0,9,101:3:0 17 2196 . G 0 . DP=19;I16=8,11,0,0,703,26383,0,0,1097,65089,0,0,312,6754,0,0;QS=3,0;MQSB=0.946915;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,117:4:0 0,12,120:4:0 17 2197 . A 0 . DP=19;I16=8,11,0,0,735,28599,0,0,1097,65089,0,0,314,6770,0,0;QS=3,0;MQSB=0.946915;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,141:4:0 0,12,125:4:0 17 2198 . G 0 . DP=19;I16=8,10,0,0,650,24206,0,0,1080,64800,0,0,307,6725,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,129:4:0 0,12,107:4:0 17 2199 . C 0 . DP=19;I16=8,11,0,0,701,26735,0,0,1097,65089,0,0,318,6862,0,0;QS=3,0;MQSB=0.946915;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,128:4:0 0,12,116:4:0 17 2200 . T 0 . DP=19;I16=8,11,0,0,710,26768,0,0,1097,65089,0,0,320,6938,0,0;QS=3,0;MQSB=0.946915;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,129:4:0 0,12,114:4:0 17 2201 . G 0 . DP=17;I16=7,10,0,0,628,23764,0,0,977,57889,0,0,324,7032,0,0;QS=3,0;MQSB=0.943335;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,6,66:2:0 0,12,119:4:0 17 2202 . G 0 . DP=17;I16=7,10,0,0,557,19249,0,0,977,57889,0,0,327,7093,0,0;QS=3,0;MQSB=0.943335;MQ0F=0 PL:DP:DV 0,33,245:11:0 0,6,57:2:0 0,12,115:4:0 17 2203 . G 0 . DP=17;I16=7,10,0,0,588,21184,0,0,977,57889,0,0,329,7123,0,0;QS=3,0;MQSB=0.943335;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,6,47:2:0 0,12,116:4:0 17 2204 . C 0 . DP=17;I16=7,10,0,0,538,18568,0,0,977,57889,0,0,331,7173,0,0;QS=3,0;MQSB=0.943335;MQ0F=0 PL:DP:DV 0,33,246:11:0 0,6,61:2:0 0,12,99:4:0 17 2205 . C 0 . DP=18;I16=7,11,0,0,628,22918,0,0,1037,61489,0,0,332,7192,0,0;QS=3,0;MQSB=0.951002;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,68:2:0 0,12,109:4:0 17 2206 . T 0 . DP=19;I16=7,12,0,0,677,25237,0,0,1097,65089,0,0,334,7230,0,0;QS=3,0;MQSB=0.957193;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,77:2:0 0,12,115:4:0 17 2207 . G 0 . DP=19;I16=7,11,0,0,663,24717,0,0,1080,64800,0,0,319,6965,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,67:2:0 0,12,110:4:0 17 2208 . G 0 . DP=21;I16=7,14,0,0,726,26038,0,0,1217,72289,0,0,340,7370,0,0;QS=3,0;MQSB=0.966484;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,110:4:0 0,12,122:4:0 17 2209 . G 0 . DP=22;I16=8,13,0,0,715,25133,0,0,1237,73369,0,0,325,7075,0,0;QS=3,0;MQSB=0.895122;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,128:5:0 0,12,117:4:0 17 2210 . G C, 0 . DP=21;I16=7,13,0,1,648,22186,25,625,1177,69769,17,289,331,7165,21,441;QS=2.95442,0.0455764,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.975265;BQB=1;MQ0F=0 PL:DP:DV 0,19,234,33,237,241:12:1 0,15,127,15,127,127:5:0 0,12,113,12,113,113:4:0 17 2211 . T 0 . DP=21;I16=7,13,0,0,724,27000,0,0,1177,69769,0,0,336,7230,0,0;QS=3,0;MQSB=0.875769;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,141:5:0 0,12,124:4:0 17 2212 . C 0 . DP=22;I16=8,14,0,0,791,29201,0,0,1254,73658,0,0,364,7850,0,0;QS=3,0;MQSB=0.985548;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,148:5:0 0,12,111:4:0 17 2213 . A 0 . DP=22;I16=8,14,0,0,767,27327,0,0,1254,73658,0,0,371,8015,0,0;QS=3,0;MQSB=0.985548;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,153:5:0 0,12,112:4:0 17 2214 . A 0 . DP=22;I16=8,14,0,0,756,26836,0,0,1254,73658,0,0,377,8159,0,0;QS=3,0;MQSB=0.985548;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,153:5:0 0,12,117:4:0 17 2215 . C 0 . DP=22;I16=8,14,0,0,815,31121,0,0,1254,73658,0,0,381,8229,0,0;QS=3,0;MQSB=0.985548;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,162:5:0 0,12,124:4:0 17 2216 . T 0 . DP=22;I16=8,14,0,0,815,31233,0,0,1254,73658,0,0,384,8272,0,0;QS=3,0;MQSB=0.985548;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,166:5:0 0,12,124:4:0 17 2217 . T 0 . DP=22;I16=8,13,0,0,741,26855,0,0,1237,73369,0,0,362,7712,0,0;QS=3,0;MQSB=0.895122;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,155:5:0 0,12,118:4:0 17 2218 . C 0 . DP=21;I16=7,14,0,0,753,27973,0,0,1194,70058,0,0,391,8423,0,0;QS=3,0;MQSB=0.975265;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,138:4:0 0,12,119:4:0 17 2219 . C 0 . DP=21;I16=7,13,0,0,757,29125,0,0,1177,69769,0,0,370,7904,0,0;QS=3,0;MQSB=0.875769;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,141:4:0 0,12,122:4:0 17 2220 . G A, 0 . DP=21;I16=6,2,1,11,256,8364,474,19128,457,26569,720,43200,141,2959,233,5071;QS=0.886504,2.1135,0;VDB=0.532753;SGB=-3.51597;RPB=0.964198;MQB=0.898397;MQSB=0.875769;BQB=0.0354359;MQ0F=0 PL:DP:DV 139,0,130,157,148,255:12:6 69,0,46,75,52,119:4:2 131,12,0,131,12,131:4:4 17 2221 . G 0 . DP=21;I16=7,13,0,0,738,27922,0,0,1177,69769,0,0,376,8078,0,0;QS=3,0;MQSB=0.875769;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,132:4:0 0,12,120:4:0 17 2222 . C 0 . DP=21;I16=7,14,0,0,746,28098,0,0,1194,70058,0,0,401,8675,0,0;QS=3,0;MQSB=0.975265;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,135:4:0 0,12,107:4:0 17 2223 . C 0 . DP=21;I16=7,14,0,0,796,30632,0,0,1194,70058,0,0,401,8671,0,0;QS=3,0;MQSB=0.975265;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,135:4:0 0,12,122:4:0 17 2224 . T 0 . DP=21;I16=7,14,0,0,811,31599,0,0,1194,70058,0,0,401,8691,0,0;QS=3,0;MQSB=0.975265;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,144:4:0 0,12,123:4:0 17 2225 . G 0 . DP=21;I16=7,14,0,0,778,29396,0,0,1194,70058,0,0,401,8735,0,0;QS=3,0;MQSB=0.975265;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,133:4:0 0,12,121:4:0 17 2226 . G 0 . DP=21;I16=7,14,0,0,727,26485,0,0,1194,70058,0,0,401,8803,0,0;QS=3,0;MQSB=0.975265;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,128:4:0 0,12,113:4:0 17 2227 . G 0 . DP=20;I16=7,12,0,0,663,23985,0,0,1117,66169,0,0,377,8269,0,0;QS=3,0;MQSB=0.879351;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,123:4:0 0,12,114:4:0 17 2228 . G C, 0 . DP=19;I16=5,12,0,1,643,24791,16,256,1020,61200,17,289,360,8020,25,625;QS=2.9603,0.0397022,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.970092;BQB=1;MQ0F=0 PL:DP:DV 0,17,255,30,255,255:11:1 0,9,95,9,95,95:3:0 0,12,121,12,121,121:4:0 17 2229 . A 0 . DP=20;I16=6,14,0,0,757,28857,0,0,1134,66458,0,0,406,9138,0,0;QS=3,0;MQSB=0.959189;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,138:4:0 0,12,122:4:0 17 2230 . A 0 . DP=20;I16=6,14,0,0,729,26985,0,0,1134,66458,0,0,409,9291,0,0;QS=3,0;MQSB=0.959189;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,141:4:0 0,12,119:4:0 17 2231 . A 0 . DP=20;I16=6,13,0,0,712,26990,0,0,1117,66169,0,0,386,8790,0,0;QS=3,0;MQSB=0.850016;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,144:4:0 0,12,124:4:0 17 2232 . C 0 . DP=20;I16=6,14,0,0,751,28657,0,0,1134,66458,0,0,412,9508,0,0;QS=3,0;MQSB=0.959189;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,130:4:0 0,12,107:4:0 17 2233 . T 0 . DP=20;I16=6,14,0,0,774,30432,0,0,1134,66458,0,0,413,9619,0,0;QS=3,0;MQSB=0.959189;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,150:4:0 0,12,117:4:0 17 2234 . G 0 . DP=20;I16=6,14,0,0,719,26621,0,0,1134,66458,0,0,412,9646,0,0;QS=3,0;MQSB=0.959189;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,145:4:0 0,12,107:4:0 17 2235 . G 0 . DP=20;I16=6,14,0,0,728,27036,0,0,1134,66458,0,0,410,9636,0,0;QS=3,0;MQSB=0.959189;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,139:4:0 0,12,104:4:0 17 2236 . G 0 . DP=19;I16=6,13,0,0,705,26985,0,0,1074,62858,0,0,409,9637,0,0;QS=3,0;MQSB=0.965977;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,142:4:0 0,9,84:3:0 17 2237 . G 0 . DP=19;I16=6,12,0,0,684,26576,0,0,1057,62569,0,0,383,9023,0,0;QS=3,0;MQSB=0.85394;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,141:4:0 0,9,86:3:0 17 2238 . C 0 . DP=19;I16=5,13,0,0,648,24496,0,0,1037,61489,0,0,381,8993,0,0;QS=3,0;MQSB=0.970092;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,108:3:0 0,9,78:3:0 17 2239 . A 0 . DP=19;I16=6,11,0,0,634,24178,0,0,997,58969,0,0,373,8935,0,0;QS=3,0;MQSB=0.85832;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,133:4:0 0,6,77:2:0 17 2240 . A 0 . DP=19;I16=6,13,0,0,731,28595,0,0,1074,62858,0,0,402,9582,0,0;QS=3,0;MQSB=0.965977;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,135:4:0 0,9,101:3:0 17 2241 . G 0 . DP=19;I16=6,12,0,0,683,26433,0,0,1057,62569,0,0,375,8951,0,0;QS=3,0;MQSB=0.85394;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,126:4:0 0,9,88:3:0 17 2242 . T 0 . DP=20;I16=5,13,0,0,635,22949,0,0,1057,62569,0,0,370,8944,0,0;QS=3,0;MQSB=0.814433;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,91:3:0 0,9,95:3:0 17 2243 . A 0 . DP=19;I16=5,14,0,0,704,26274,0,0,1074,62858,0,0,395,9585,0,0;QS=3,0;MQSB=0.933727;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,107:3:0 0,9,99:3:0 17 2244 . T 0 . DP=19;I16=5,14,0,0,681,25263,0,0,1074,62858,0,0,395,9609,0,0;QS=3,0;MQSB=0.933727;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,89:3:0 0,9,98:3:0 17 2245 . C 0 . DP=19;I16=5,14,0,0,722,27846,0,0,1074,62858,0,0,394,9592,0,0;QS=3,0;MQSB=0.933727;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,98:3:0 0,9,88:3:0 17 2246 . A 0 . DP=18;I16=5,12,0,0,597,21693,0,0,997,58969,0,0,367,8861,0,0;QS=3,0;MQSB=0.818731;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,95:3:0 0,6,72:2:0 17 2247 . C 0 . DP=17;I16=4,13,0,0,639,24373,0,0,954,55658,0,0,391,9391,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,105:3:0 0,6,71:2:0 17 2248 . C 0 . DP=17;I16=4,13,0,0,669,26863,0,0,954,55658,0,0,390,9306,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,90:3:0 0,6,75:2:0 17 2249 . A 0 . DP=17;I16=4,13,0,0,677,27371,0,0,954,55658,0,0,389,9231,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,116:3:0 0,6,80:2:0 17 2250 . G 0 . DP=17;I16=4,13,0,0,654,25800,0,0,954,55658,0,0,388,9166,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,100:3:0 0,6,78:2:0 17 2251 . A 0 . DP=17;I16=4,13,0,0,673,27327,0,0,954,55658,0,0,387,9111,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,112:3:0 0,6,80:2:0 17 2252 . G 0 . DP=17;I16=4,12,0,0,647,26249,0,0,937,55369,0,0,361,8441,0,0;QS=3,0;MQSB=0.767432;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,113:3:0 0,6,73:2:0 17 2253 . A 0 . DP=17;I16=4,13,0,0,641,24643,0,0,954,55658,0,0,385,9031,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,111:3:0 0,6,73:2:0 17 2254 . T 0 . DP=17;I16=4,12,0,0,615,23821,0,0,937,55369,0,0,358,8332,0,0;QS=3,0;MQSB=0.767432;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,112:3:0 0,6,73:2:0 17 2255 . G 0 . DP=17;I16=4,13,0,0,677,27243,0,0,954,55658,0,0,380,8844,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,115:3:0 0,6,71:2:0 17 2256 . A 0 . DP=17;I16=4,13,0,0,656,26088,0,0,954,55658,0,0,377,8741,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,110:3:0 0,6,78:2:0 17 2257 . G 0 . DP=17;I16=4,12,0,0,627,24863,0,0,937,55369,0,0,349,8023,0,0;QS=3,0;MQSB=0.767432;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,115:3:0 0,6,74:2:0 17 2258 . C 0 . DP=17;I16=4,13,0,0,667,26403,0,0,954,55658,0,0,371,8565,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,109:3:0 0,6,70:2:0 17 2259 . T 0 . DP=17;I16=4,13,0,0,646,25334,0,0,954,55658,0,0,368,8492,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,116:3:0 0,6,79:2:0 17 2260 . T 0 . DP=17;I16=4,13,0,0,638,24178,0,0,954,55658,0,0,365,8429,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,115:3:0 0,6,72:2:0 17 2261 . T 0 . DP=17;I16=4,13,0,0,633,23799,0,0,954,55658,0,0,362,8376,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,105:3:0 0,6,73:2:0 17 2262 . A 0 . DP=17;I16=4,13,0,0,628,23438,0,0,954,55658,0,0,359,8333,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,36,253:12:0 0,9,112:3:0 0,6,74:2:0 17 2263 . T 0 . DP=17;I16=4,13,0,0,631,23673,0,0,954,55658,0,0,355,8251,0,0;QS=3,0;MQSB=0.90252;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,112:3:0 0,6,74:2:0 17 2264 . A 0 . DP=17;I16=4,12,0,0,623,24371,0,0,937,55369,0,0,326,7556,0,0;QS=3,0;MQSB=0.767432;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,114:3:0 0,6,75:2:0 17 2265 . A 0 . DP=18;I16=4,13,0,0,642,24718,0,0,997,58969,0,0,320,7400,0,0;QS=3,0;MQSB=0.762744;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,113:3:0 0,6,75:2:0 17 2266 . A 0 . DP=19;I16=5,14,0,0,656,23962,0,0,1074,62858,0,0,337,7745,0,0;QS=3,0;MQSB=0.933727;MQ0F=0 PL:DP:DV 0,39,252:13:0 0,12,128:4:0 0,6,75:2:0 17 2267 . A 0 . DP=20;I16=6,14,0,0,712,26052,0,0,1134,66458,0,0,332,7582,0,0;QS=3,0;MQSB=0.959189;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,134:4:0 0,9,102:3:0 17 2268 . A 0 . DP=20;I16=6,14,0,0,741,27841,0,0,1134,66458,0,0,327,7391,0,0;QS=3,0;MQSB=0.959189;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,135:4:0 0,9,100:3:0 17 2269 . T 0 . DP=20;I16=6,13,0,0,681,24751,0,0,1074,62858,0,0,318,7206,0,0;QS=3,0;MQSB=0.965977;MQ0F=0 PL:DP:DV 0,36,246:12:0 0,12,137:4:0 0,9,111:3:0 17 2270 . A 0 . DP=19;I16=6,12,0,0,652,24154,0,0,1057,62569,0,0,300,6750,0,0;QS=3,0;MQSB=0.85394;MQ0F=0 PL:DP:DV 0,33,244:11:0 0,12,134:4:0 0,9,112:3:0 17 2271 . A 0 . DP=17;I16=6,11,0,0,654,25406,0,0,954,55658,0,0,316,6944,0,0;QS=3,0;MQSB=0.980001;MQ0F=0 PL:DP:DV 0,30,243:10:0 0,12,142:4:0 0,9,111:3:0 17 2272 . T 0 . DP=17;I16=6,10,0,0,604,22908,0,0,937,55369,0,0,297,6525,0,0;QS=3,0;MQSB=0.863243;MQ0F=0 PL:DP:DV 0,27,238:9:0 0,12,137:4:0 0,9,111:3:0 17 2273 . G 0 . DP=17;I16=6,10,0,0,581,22129,0,0,937,55369,0,0,295,6411,0,0;QS=3,0;MQSB=0.863243;MQ0F=0 PL:DP:DV 0,27,240:9:0 0,12,123:4:0 0,9,115:3:0 17 2274 . G 0 . DP=18;I16=6,11,0,0,621,23109,0,0,997,58969,0,0,293,6313,0,0;QS=3,0;MQSB=0.85832;MQ0F=0 PL:DP:DV 0,30,248:10:0 0,12,132:4:0 0,9,110:3:0 17 2275 . T 0 . DP=18;I16=6,11,0,0,624,23152,0,0,997,58969,0,0,292,6232,0,0;QS=3,0;MQSB=0.85832;MQ0F=0 PL:DP:DV 0,30,249:10:0 0,12,126:4:0 0,9,113:3:0 17 2276 . G 0 . DP=20;I16=7,12,0,0,710,26848,0,0,1074,62858,0,0,303,6313,0,0;QS=3,0;MQSB=0.985816;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,129:4:0 0,9,110:3:0 17 2277 . C 0 . DP=21;I16=8,13,0,0,797,30699,0,0,1194,70058,0,0,303,6247,0,0;QS=3,0;MQSB=0.989565;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,151:5:0 0,12,143:4:0 17 2278 . T 0 . DP=21;I16=7,13,0,0,736,27790,0,0,1157,68689,0,0,279,5581,0,0;QS=3,0;MQSB=0.962269;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,133:4:0 0,12,148:4:0 17 2279 . A 0 . DP=20;I16=8,12,0,0,723,27047,0,0,1134,66458,0,0,306,6190,0,0;QS=3,0;MQSB=0.993326;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,155:5:0 0,12,144:4:0 17 2280 . G 0 . DP=21;I16=8,12,0,0,766,29776,0,0,1177,69769,0,0,299,6085,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,166:5:0 0,12,144:4:0 17 2281 . C 0 . DP=22;I16=8,13,0,0,784,29748,0,0,1237,73369,0,0,301,6037,0,0;QS=3,0;MQSB=0.895122;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,165:5:0 0,12,140:4:0 17 2282 . T 0 . DP=22;I16=8,14,0,0,789,29699,0,0,1254,73658,0,0,310,6054,0,0;QS=3,0;MQSB=0.985548;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,162:5:0 0,12,150:4:0 17 2283 . G 0 . DP=22;I16=8,14,0,0,798,29774,0,0,1254,73658,0,0,312,6054,0,0;QS=3,0;MQSB=0.985548;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,158:5:0 0,12,142:4:0 17 2284 . G 0 . DP=22;I16=7,14,0,0,760,28408,0,0,1194,70058,0,0,294,5664,0,0;QS=3,0;MQSB=0.975265;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,130:4:0 0,12,141:4:0 17 2285 . G 0 . DP=23;I16=9,14,0,0,844,31592,0,0,1314,77258,0,0,311,5909,0,0;QS=3,0;MQSB=0.992095;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,152:5:0 0,12,144:4:0 17 2286 . C 0 . DP=23;I16=9,14,0,0,854,32244,0,0,1314,77258,0,0,311,5869,0,0;QS=3,0;MQSB=0.992095;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,155:5:0 0,12,130:4:0 17 2287 . A 0 . DP=23;I16=9,14,0,0,818,30288,0,0,1314,77258,0,0,311,5869,0,0;QS=3,0;MQSB=0.992095;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,166:5:0 0,12,143:4:0 17 2288 . T 0 . DP=22;I16=8,14,0,0,794,29190,0,0,1254,73658,0,0,312,5908,0,0;QS=3,0;MQSB=0.985548;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,158:5:0 0,12,142:4:0 17 2289 . G 0 . DP=21;I16=8,13,0,0,723,25835,0,0,1237,73369,0,0,314,5984,0,0;QS=3,0;MQSB=0.895122;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,154:5:0 0,12,126:4:0 17 2290 . G 0 . DP=20;I16=7,13,0,0,748,28318,0,0,1177,69769,0,0,318,6094,0,0;QS=3,0;MQSB=0.875769;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,149:5:0 0,9,114:3:0 17 2291 . T 0 . DP=20;I16=7,13,0,0,731,27165,0,0,1177,69769,0,0,322,6186,0,0;QS=3,0;MQSB=0.875769;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,151:5:0 0,9,112:3:0 17 2292 . G 0 . DP=20;I16=7,13,0,0,749,28747,0,0,1177,69769,0,0,325,6259,0,0;QS=3,0;MQSB=0.875769;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,156:5:0 0,9,109:3:0 17 2293 . G 0 . DP=20;I16=7,13,0,0,739,27903,0,0,1177,69769,0,0,327,6311,0,0;QS=3,0;MQSB=0.875769;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,156:5:0 0,9,112:3:0 17 2294 . C 0 . DP=21;I16=7,14,0,0,775,29453,0,0,1237,73369,0,0,329,6391,0,0;QS=3,0;MQSB=0.872525;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,169:5:0 0,9,114:3:0 17 2295 . T 0 . DP=21;I16=7,14,0,0,773,29209,0,0,1237,73369,0,0,331,6451,0,0;QS=3,0;MQSB=0.872525;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,164:5:0 0,9,115:3:0 17 2296 . T 0 . DP=21;I16=7,14,0,0,756,27780,0,0,1237,73369,0,0,333,6543,0,0;QS=3,0;MQSB=0.872525;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,160:5:0 0,9,106:3:0 17 2297 . G 0 . DP=20;I16=7,13,0,0,736,27692,0,0,1177,69769,0,0,336,6666,0,0;QS=3,0;MQSB=0.875769;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,165:5:0 0,6,74:2:0 17 2298 . C 0 . DP=21;I16=7,14,0,0,778,29300,0,0,1237,73369,0,0,339,6819,0,0;QS=3,0;MQSB=0.872525;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,139:5:0 0,9,106:3:0 17 2299 . A 0 . DP=21;I16=7,14,0,0,714,25282,0,0,1237,73369,0,0,343,7003,0,0;QS=3,0;MQSB=0.872525;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,128:5:0 0,9,110:3:0 17 2300 . C 0 . DP=21;I16=7,13,0,0,687,24749,0,0,1177,69769,0,0,326,6768,0,0;QS=3,0;MQSB=0.875769;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,148:5:0 0,9,109:3:0 17 2301 . C 0 . DP=23;I16=7,15,0,0,802,30292,0,0,1266,74210,0,0,349,7363,0,0;QS=3,0;MQSB=0.970024;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,147:5:0 0,9,116:3:0 17 2302 . T 0 . DP=23;I16=7,16,0,0,860,32956,0,0,1326,77810,0,0,354,7496,0,0;QS=3,0;MQSB=0.964916;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,166:5:0 0,12,150:4:0 17 2303 . G 0 . DP=23;I16=7,16,0,0,817,29823,0,0,1326,77810,0,0,356,7604,0,0;QS=3,0;MQSB=0.964916;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,151:5:0 0,12,139:4:0 17 2304 . T 0 . DP=23;I16=7,16,0,0,802,28628,0,0,1326,77810,0,0,357,7691,0,0;QS=3,0;MQSB=0.964916;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,148:5:0 0,12,140:4:0 17 2305 . A 0 . DP=22;I16=7,14,0,0,725,25585,0,0,1237,73369,0,0,355,7791,0,0;QS=3,0;MQSB=0.872525;MQ0F=0 PL:DP:DV 0,36,248:12:0 0,15,155:5:0 0,12,129:4:0 17 2306 . A 0 . DP=21;I16=7,14,0,0,727,26841,0,0,1206,70610,0,0,361,7899,0,0;QS=3,0;MQSB=0.975265;MQ0F=0 PL:DP:DV 0,36,246:12:0 0,15,168:5:0 0,12,130:4:0 17 2307 . T 0 . DP=21;I16=7,14,0,0,751,27427,0,0,1206,70610,0,0,362,7964,0,0;QS=3,0;MQSB=0.975265;MQ0F=0 PL:DP:DV 0,36,251:12:0 0,15,165:5:0 0,12,141:4:0 17 2308 . C 0 . DP=21;I16=7,14,0,0,735,26675,0,0,1206,70610,0,0,363,8051,0,0;QS=3,0;MQSB=0.975265;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,144:5:0 0,12,146:4:0 17 2309 . C A, 0 . DP=19;I16=7,11,0,1,604,21364,16,256,1057,62569,29,841,358,8094,8,64;QS=2.95676,0.0432432,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.985816;BQB=1;MQ0F=0 PL:DP:DV 0,20,224,33,227,230:12:1 0,9,94,9,94,94:3:0 0,12,138,12,138,138:4:0 17 2310 . C 0 . DP=18;I16=6,12,0,0,668,25392,0,0,1049,62041,0,0,370,8282,0,0;QS=3,0;MQSB=0.961295;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,80:2:0 0,12,139:4:0 17 2311 . A 0 . DP=19;I16=7,12,0,0,707,26855,0,0,1109,65641,0,0,373,8371,0,0;QS=3,0;MQSB=0.957193;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,78:2:0 0,12,137:4:0 17 2312 . G 0 . DP=20;I16=7,12,0,0,736,29118,0,0,1109,65641,0,0,364,8306,0,0;QS=3,0;MQSB=0.957193;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,82:2:0 0,9,122:3:0 17 2313 . C 0 . DP=20;I16=7,13,0,0,723,27231,0,0,1169,69241,0,0,382,8596,0,0;QS=3,0;MQSB=0.962269;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,76:2:0 0,12,130:4:0 17 2314 . A 0 . DP=21;I16=7,12,0,0,630,22184,0,0,1109,65641,0,0,372,8510,0,0;QS=3,0;MQSB=0.957193;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,78:2:0 0,9,115:3:0 17 2315 . C 0 . DP=23;I16=7,14,0,0,739,26861,0,0,1229,72841,0,0,392,8892,0,0;QS=3,0;MQSB=0.966484;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,71:2:0 0,12,135:4:0 17 2316 . T 0 . DP=23;I16=8,15,0,0,826,30690,0,0,1349,80041,0,0,408,9102,0,0;QS=3,0;MQSB=0.967216;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,105:3:0 0,15,170:5:0 17 2317 . T 0 . DP=24;I16=7,16,0,0,766,27014,0,0,1349,80041,0,0,411,9211,0,0;QS=3,0;MQSB=0.973027;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,78:2:0 0,15,170:5:0 17 2318 . T 0 . DP=24;I16=8,14,0,0,757,27433,0,0,1320,79200,0,0,379,8449,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,91:3:0 0,15,165:5:0 17 2319 . G 0 . DP=24;I16=6,16,0,0,827,31577,0,0,1289,76441,0,0,398,8882,0,0;QS=3,0;MQSB=0.97584;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,3,43:1:0 0,15,167:5:0 17 2320 . G 0 . DP=23;I16=7,16,0,0,803,29077,0,0,1349,80041,0,0,435,9675,0,0;QS=3,0;MQSB=0.973027;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,87:3:0 0,15,157:5:0 17 2321 . G 0 . DP=23;I16=7,16,0,0,804,29368,0,0,1349,80041,0,0,442,9840,0,0;QS=3,0;MQSB=0.973027;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,79:3:0 0,15,169:5:0 17 2322 . A 0 . DP=24;I16=7,17,0,0,860,32116,0,0,1409,83641,0,0,449,10027,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,95:3:0 0,15,178:5:0 17 2323 . G 0 . DP=24;I16=7,17,0,0,863,31887,0,0,1409,83641,0,0,457,10237,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,88:3:0 0,15,153:5:0 17 2324 . G 0 . DP=24;I16=7,16,0,0,786,27932,0,0,1349,80041,0,0,462,10416,0,0;QS=3,0;MQSB=0.973027;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,88:3:0 0,15,161:5:0 17 2325 . C 0 . DP=24;I16=7,15,0,0,730,25576,0,0,1289,76441,0,0,427,9625,0,0;QS=3,0;MQSB=0.970024;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,102:3:0 0,12,130:4:0 17 2326 . C 0 . DP=24;I16=7,15,0,0,709,23523,0,0,1320,79200,0,0,426,9498,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,241:15:0 0,9,95:3:0 0,12,133:4:0 17 2327 . G 0 . DP=24;I16=7,17,0,0,817,29275,0,0,1409,83641,0,0,481,10891,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,81:3:0 0,15,158:5:0 17 2328 . A 0 . DP=24;I16=6,17,0,0,814,29804,0,0,1349,80041,0,0,461,10427,0,0;QS=3,0;MQSB=0.978183;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,82:3:0 0,15,172:5:0 17 2329 . G 0 . DP=23;I16=6,16,0,0,755,27641,0,0,1289,76441,0,0,477,11005,0,0;QS=3,0;MQSB=0.97584;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,63:2:0 0,15,160:5:0 17 2330 . C 0 . DP=24;I16=7,17,0,0,875,33131,0,0,1409,83641,0,0,498,11424,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,87:3:0 0,18,181:6:0 17 2331 . T 0 . DP=24;I16=7,17,0,0,862,31882,0,0,1409,83641,0,0,505,11635,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,106:3:0 0,18,200:6:0 17 2332 . A 0 . DP=25;I16=8,17,0,0,926,35412,0,0,1469,87241,0,0,512,11864,0,0;QS=3,0;MQSB=0.973216;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,97:3:0 0,18,204:6:0 17 2333 . G 0 . DP=26;I16=8,17,0,0,923,35129,0,0,1469,87241,0,0,494,11436,0,0;QS=3,0;MQSB=0.973216;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,97:3:0 0,18,189:6:0 17 2334 . G 0 . DP=26;I16=8,18,0,0,928,34574,0,0,1529,90841,0,0,527,12277,0,0;QS=3,0;MQSB=0.975611;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,93:3:0 0,21,202:7:0 17 2335 . A 0 . DP=26;I16=8,18,0,0,967,36793,0,0,1529,90841,0,0,535,12513,0,0;QS=3,0;MQSB=0.975611;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,105:3:0 0,21,214:7:0 17 2336 . G 0 . DP=26;I16=8,18,0,0,962,36346,0,0,1529,90841,0,0,543,12769,0,0;QS=3,0;MQSB=0.975611;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,92:3:0 0,21,204:7:0 17 2337 . G 0 . DP=26;I16=7,18,0,0,895,32981,0,0,1469,87241,0,0,527,12465,0,0;QS=3,0;MQSB=0.977814;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,74:2:0 0,21,204:7:0 17 2338 . A 0 . DP=26;I16=8,18,0,0,892,31758,0,0,1529,90841,0,0,556,13186,0,0;QS=3,0;MQSB=0.975611;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,87:3:0 0,21,194:7:0 17 2339 . T 0 . DP=26;I16=7,18,0,0,827,28921,0,0,1469,87241,0,0,537,12769,0,0;QS=3,0;MQSB=0.977814;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,55:2:0 0,21,200:7:0 17 2340 . C 0 . DP=25;I16=7,18,0,0,792,25816,0,0,1469,87241,0,0,541,12893,0,0;QS=3,0;MQSB=0.977814;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,57:2:0 0,21,173:7:0 17 2341 . G 0 . DP=25;I16=6,17,0,0,771,26477,0,0,1349,80041,0,0,494,11732,0,0;QS=3,0;MQSB=0.978183;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,3,41:1:0 0,21,186:7:0 17 2342 . T 0 . DP=24;I16=6,17,0,0,863,32711,0,0,1349,80041,0,0,523,12459,0,0;QS=3,0;MQSB=0.978183;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,3,43:1:0 0,21,214:7:0 17 2343 . T 0 . DP=24;I16=6,16,0,0,770,28230,0,0,1289,76441,0,0,504,12032,0,0;QS=3,0;MQSB=0.97584;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,3,44:1:0 0,21,213:7:0 17 2344 . T 0 . DP=24;I16=7,17,0,0,843,30483,0,0,1409,83641,0,0,552,13124,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,56:2:0 0,21,202:7:0 17 2345 . G 0 . DP=24;I16=7,17,0,0,897,34121,0,0,1409,83641,0,0,554,13162,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,77:2:0 0,21,206:7:0 17 2346 . A 0 . DP=24;I16=7,17,0,0,908,34818,0,0,1409,83641,0,0,556,13212,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,68:2:0 0,21,220:7:0 17 2347 . G 0 . DP=24;I16=6,17,0,0,824,30406,0,0,1349,80041,0,0,533,12649,0,0;QS=3,0;MQSB=0.978183;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,3,39:1:0 0,21,190:7:0 17 2348 . T 0 . DP=24;I16=7,16,0,0,745,25653,0,0,1349,80041,0,0,544,13072,0,0;QS=3,0;MQSB=0.973027;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,64:2:0 0,18,183:6:0 17 2349 . C 0 . DP=24;I16=6,17,0,0,804,29224,0,0,1349,80041,0,0,534,12656,0,0;QS=3,0;MQSB=0.978183;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,3,36:1:0 0,21,200:7:0 17 2350 . C 0 . DP=25;I16=7,17,0,0,869,31905,0,0,1409,83641,0,0,534,12652,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,63:2:0 0,21,198:7:0 17 2351 . A 0 . DP=25;I16=8,17,0,0,945,36169,0,0,1469,87241,0,0,559,13237,0,0;QS=3,0;MQSB=0.973216;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,74:2:0 0,21,222:7:0 17 2352 . G 0 . DP=25;I16=7,17,0,0,893,34005,0,0,1409,83641,0,0,533,12539,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,3,41:1:0 0,21,207:7:0 17 2353 . C 0 . DP=24;I16=7,17,0,0,866,31864,0,0,1409,83641,0,0,531,12435,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,3,41:1:0 0,21,205:7:0 17 2354 . A 0 . DP=24;I16=7,17,0,0,880,33206,0,0,1409,83641,0,0,529,12351,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,3,43:1:0 0,21,214:7:0 17 2355 . G 0 . DP=24;I16=7,17,0,0,871,32261,0,0,1409,83641,0,0,526,12238,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,3,39:1:0 0,21,195:7:0 17 2356 . T 0 . DP=24;I16=7,17,0,0,887,33305,0,0,1409,83641,0,0,521,12047,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,3,43:1:0 0,21,219:7:0 17 2357 . T 0 . DP=24;I16=7,17,0,0,899,34225,0,0,1409,83641,0,0,516,11878,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,3,43:1:0 0,21,220:7:0 17 2358 . T 0 . DP=24;I16=7,17,0,0,913,34891,0,0,1409,83641,0,0,510,11680,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,3,40:1:0 0,21,218:7:0 17 2359 . G 0 . DP=25;I16=7,17,0,0,898,34048,0,0,1409,83641,0,0,503,11451,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,3,39:1:0 0,21,209:7:0 17 2360 . A 0 . DP=25;I16=7,18,0,0,959,37113,0,0,1469,87241,0,0,514,11552,0,0;QS=3,0;MQSB=0.977814;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,72:2:0 0,21,222:7:0 17 2361 . G 0 . DP=25;I16=7,17,0,0,850,30946,0,0,1409,83641,0,0,482,10726,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,67:2:0 0,18,168:6:0 17 2362 . A 0 . DP=25;I16=7,17,0,0,762,25482,0,0,1409,83641,0,0,475,10547,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,51:2:0 0,18,159:6:0 17 2363 . C 0 . DP=25;I16=7,18,0,0,875,31837,0,0,1469,87241,0,0,493,11015,0,0;QS=3,0;MQSB=0.977814;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,65:2:0 0,21,187:7:0 17 2364 . C 0 . DP=25;I16=7,18,0,0,927,34965,0,0,1469,87241,0,0,486,10880,0,0;QS=3,0;MQSB=0.977814;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,64:2:0 0,21,201:7:0 17 2365 . A 0 . DP=24;I16=7,17,0,0,908,34754,0,0,1409,83641,0,0,479,10717,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,65:2:0 0,21,221:7:0 17 2366 . G 0 . DP=25;I16=7,17,0,0,912,35074,0,0,1440,86400,0,0,447,9951,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,58:2:0 0,21,206:7:0 17 2367 . C 0 . DP=25;I16=7,17,0,0,872,32088,0,0,1409,83641,0,0,440,9782,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,3,36:1:0 0,21,197:7:0 17 2368 . C 0 . DP=24;I16=6,17,0,0,871,33801,0,0,1349,80041,0,0,434,9634,0,0;QS=3,0;MQSB=0.978183;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,3,39:1:0 0,18,180:6:0 17 2369 . T 0 . DP=24;I16=6,18,0,0,877,33009,0,0,1409,83641,0,0,452,10082,0,0;QS=3,0;MQSB=0.980199;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,49:2:0 0,18,179:6:0 17 2370 . G 0 . DP=24;I16=6,18,0,0,899,34289,0,0,1409,83641,0,0,445,9927,0,0;QS=3,0;MQSB=0.980199;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,56:2:0 0,18,179:6:0 17 2371 . G 0 . DP=24;I16=6,18,0,0,880,33088,0,0,1409,83641,0,0,438,9794,0,0;QS=3,0;MQSB=0.980199;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,51:2:0 0,18,177:6:0 17 2372 . C 0 . DP=24;I16=6,18,0,0,827,29615,0,0,1409,83641,0,0,431,9683,0,0;QS=3,0;MQSB=0.980199;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,47:2:0 0,18,163:6:0 17 2373 . C 0 . DP=24;I16=6,18,0,0,886,33212,0,0,1409,83641,0,0,424,9594,0,0;QS=3,0;MQSB=0.980199;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,58:2:0 0,18,166:6:0 17 2374 . A 0 . DP=23;I16=6,17,0,0,844,31230,0,0,1349,80041,0,0,417,9477,0,0;QS=3,0;MQSB=0.978183;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,66:2:0 0,18,177:6:0 17 2375 . A 0 . DP=23;I16=5,17,0,0,788,28340,0,0,1289,76441,0,0,409,9333,0,0;QS=3,0;MQSB=0.981001;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,64:2:0 0,18,171:6:0 17 2376 . T 0 . DP=22;I16=5,17,0,0,778,27804,0,0,1289,76441,0,0,401,9161,0,0;QS=3,0;MQSB=0.981001;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,62:2:0 0,18,162:6:0 17 2377 . A 0 . DP=21;I16=4,17,0,0,704,24488,0,0,1229,72841,0,0,394,9008,0,0;QS=3,0;MQSB=0.984085;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,49:2:0 0,15,135:5:0 17 2378 . C 0 . DP=20;I16=4,16,0,0,626,20138,0,0,1169,69241,0,0,388,8872,0,0;QS=3,0;MQSB=0.982301;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,3,15:1:0 0,15,108:5:0 17 2379 . G 0 . DP=20;I16=4,16,0,0,714,25924,0,0,1169,69241,0,0,382,8752,0,0;QS=3,0;MQSB=0.982301;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,3,34:1:0 0,15,140:5:0 17 2380 . G 0 . DP=19;I16=4,14,0,0,672,25672,0,0,1049,62041,0,0,352,8022,0,0;QS=3,0;MQSB=0.977696;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,3,18:1:0 0,15,134:5:0 17 2381 . C 0 . DP=18;I16=4,14,0,0,676,25626,0,0,1049,62041,0,0,373,8555,0,0;QS=3,0;MQSB=0.977696;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,3,28:1:0 0,15,133:5:0 17 2382 . A 0 . DP=19;I16=5,14,0,0,669,23995,0,0,1109,65641,0,0,369,8475,0,0;QS=3,0;MQSB=0.97357;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,3,28:1:0 0,15,131:5:0 17 2383 . A 0 . DP=19;I16=5,14,0,0,686,25162,0,0,1109,65641,0,0,365,8359,0,0;QS=3,0;MQSB=0.97357;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,3,26:1:0 0,15,140:5:0 17 2384 . A 0 . DP=19;I16=5,14,0,0,649,22943,0,0,1109,65641,0,0,360,8210,0,0;QS=3,0;MQSB=0.97357;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,3,13:1:0 0,15,132:5:0 17 2385 . A 0 . DP=18;I16=4,14,0,0,643,23235,0,0,1049,62041,0,0,356,8078,0,0;QS=3,0;MQSB=0.977696;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,3,38:1:0 0,15,134:5:0 17 2386 . C 0 . DP=18;I16=4,14,0,0,652,24008,0,0,1049,62041,0,0,351,7913,0,0;QS=3,0;MQSB=0.977696;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,3,25:1:0 0,15,130:5:0 17 2387 . C 0 . DP=18;I16=4,14,0,0,667,25007,0,0,1049,62041,0,0,345,7717,0,0;QS=3,0;MQSB=0.977696;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,3,25:1:0 0,15,128:5:0 17 2388 . C 0 . DP=18;I16=4,14,0,0,691,26659,0,0,1049,62041,0,0,339,7541,0,0;QS=3,0;MQSB=0.977696;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,3,39:1:0 0,15,134:5:0 17 2389 . A 0 . DP=19;I16=5,14,0,0,682,24912,0,0,1109,65641,0,0,333,7385,0,0;QS=3,0;MQSB=0.97357;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,3,35:1:0 0,15,140:5:0 17 2390 . G 0 . DP=18;I16=5,13,0,0,642,23418,0,0,1049,62041,0,0,328,7200,0,0;QS=3,0;MQSB=0.970092;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,3,27:1:0 0,15,126:5:0 17 2391 . T 0 . DP=18;I16=5,12,0,0,624,23122,0,0,989,58441,0,0,298,6412,0,0;QS=2,0;MQSB=0.965874;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,0,0:0:0 0,15,140:5:0 17 2392 . C 0 . DP=18;I16=5,12,0,0,660,25910,0,0,989,58441,0,0,291,6171,0,0;QS=2,0;MQSB=0.965874;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,0,0:0:0 0,15,131:5:0 17 2393 . T 0 . DP=18;I16=5,13,0,0,669,25099,0,0,1049,62041,0,0,309,6577,0,0;QS=3,0;MQSB=0.970092;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,3,38:1:0 0,15,134:5:0 17 2394 . C 0 . DP=17;I16=5,12,0,0,653,25323,0,0,989,58441,0,0,303,6379,0,0;QS=3,0;MQSB=0.965874;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,3,29:1:0 0,15,133:5:0 17 2395 . T 0 . DP=17;I16=5,12,0,0,613,22621,0,0,989,58441,0,0,297,6201,0,0;QS=3,0;MQSB=0.965874;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,3,19:1:0 0,15,128:5:0 17 2396 . A 0 . DP=17;I16=5,11,0,0,584,21426,0,0,929,54841,0,0,266,5418,0,0;QS=2,0;MQSB=0.960687;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,0,0:0:0 0,15,130:5:0 17 2397 . C 0 . DP=18;I16=5,13,0,0,650,24046,0,0,1049,62041,0,0,284,5856,0,0;QS=3,0;MQSB=0.970092;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,3,18:1:0 0,15,129:5:0 17 2398 . A 0 . DP=18;I16=5,13,0,0,617,21907,0,0,1049,62041,0,0,278,5692,0,0;QS=3,0;MQSB=0.970092;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,3,19:1:0 0,15,128:5:0 17 2399 . A 0 . DP=17;I16=5,11,0,0,592,22176,0,0,929,54841,0,0,248,4926,0,0;QS=2,0;MQSB=0.960687;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,0,0:0:0 0,12,115:4:0 17 2400 . A 0 . DP=16;I16=5,11,0,0,576,21010,0,0,929,54841,0,0,269,5431,0,0;QS=3,0;MQSB=0.960687;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,3,26:1:0 0,9,99:3:0 17 2401 . A 0 . DP=17;I16=6,11,0,0,594,21126,0,0,989,58441,0,0,265,5331,0,0;QS=3,0;MQSB=0.955563;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,3,23:1:0 0,9,98:3:0 17 2402 . A 0 . DP=17;I16=6,11,0,0,606,21986,0,0,989,58441,0,0,262,5252,0,0;QS=3,0;MQSB=0.955563;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,3,27:1:0 0,9,99:3:0 17 2403 . A 0 . DP=17;I16=6,11,0,0,608,22130,0,0,989,58441,0,0,259,5195,0,0;QS=3,0;MQSB=0.955563;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,3,21:1:0 0,9,97:3:0 17 2404 . T 0 . DP=17;I16=6,11,0,0,567,19449,0,0,989,58441,0,0,256,5160,0,0;QS=3,0;MQSB=0.955563;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,3,14:1:0 0,9,87:3:0 17 2405 . A 0 . DP=18;I16=7,11,0,0,618,21666,0,0,1049,62041,0,0,253,5147,0,0;QS=3,0;MQSB=0.951002;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,3,17:1:0 0,9,88:3:0 17 2406 . C 0 . DP=18;I16=7,11,0,0,676,25664,0,0,1049,62041,0,0,250,5108,0,0;QS=3,0;MQSB=0.951002;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,3,27:1:0 0,9,90:3:0 17 2407 . A 0 . DP=19;I16=8,11,0,0,673,24197,0,0,1109,65641,0,0,246,5046,0,0;QS=3,0;MQSB=0.946915;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,3,34:1:0 0,9,89:3:0 17 2408 . A 0 . DP=18;I16=8,10,0,0,619,21951,0,0,1049,62041,0,0,243,4961,0,0;QS=3,0;MQSB=0.938795;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,3,22:1:0 0,6,73:2:0 17 2409 . A 0 . DP=17;I16=8,9,0,0,615,22687,0,0,1020,61200,0,0,240,4852,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,3,39:1:0 0,6,74:2:0 17 2410 . A 0 . DP=17;I16=8,9,0,0,604,21938,0,0,1020,61200,0,0,237,4769,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,3,39:1:0 0,6,73:2:0 17 2411 . A 0 . DP=16;I16=7,9,0,0,567,20551,0,0,960,57600,0,0,235,4711,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,3,25:1:0 0,6,70:2:0 17 2412 . A 0 . DP=15;I16=7,8,0,0,527,18903,0,0,900,54000,0,0,234,4676,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,3,21:1:0 0,6,70:2:0 17 2413 . C 0 . DP=15;I16=7,8,0,0,559,21161,0,0,900,54000,0,0,233,4663,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,3,22:1:0 0,6,72:2:0 17 2414 . A 0 . DP=16;I16=8,8,0,0,570,20822,0,0,929,54841,0,0,232,4672,0,0;QS=3,0;MQSB=0.915545;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,3,25:1:0 0,6,73:2:0 17 2415 . A 0 . DP=16;I16=9,7,0,0,574,20768,0,0,929,54841,0,0,232,4652,0,0;QS=3,0;MQSB=0.933674;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,3,30:1:0 0,6,64:2:0 17 2416 . C 0 . DP=17;I16=8,8,0,0,576,21424,0,0,960,57600,0,0,231,4649,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,42:2:0 0,6,68:2:0 17 2417 . T 0 . DP=17;I16=10,7,0,0,612,22740,0,0,958,55682,0,0,235,4627,0,0;QS=3,0;MQSB=0.79189;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,57:2:0 0,9,92:3:0 17 2418 . A 0 . DP=17;I16=10,7,0,0,624,23210,0,0,958,55682,0,0,238,4626,0,0;QS=3,0;MQSB=0.79189;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,62:2:0 0,9,105:3:0 17 2419 . G 0 . DP=19;I16=11,7,0,0,651,24113,0,0,1018,59282,0,0,241,4651,0,0;QS=3,0;MQSB=0.817948;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,51:2:0 0,12,126:4:0 17 2420 . C 0 . DP=19;I16=11,8,0,0,663,23999,0,0,1078,62882,0,0,246,4704,0,0;QS=3,0;MQSB=0.803979;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,44:2:0 0,12,127:4:0 17 2421 . C 0 . DP=19;I16=10,8,0,0,657,24779,0,0,1049,62041,0,0,244,4738,0,0;QS=3,0;MQSB=0.938795;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,45:2:0 0,12,126:4:0 17 2422 . A 0 . DP=18;I16=11,6,0,0,647,24747,0,0,958,55682,0,0,238,4538,0,0;QS=3,0;MQSB=0.833753;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,3,33:1:0 0,12,137:4:0 17 2423 . G 0 . DP=18;I16=11,6,0,0,634,24250,0,0,958,55682,0,0,258,4972,0,0;QS=3,0;MQSB=0.833753;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,6,54:2:0 0,12,126:4:0 17 2424 . G 0 . DP=18;I16=10,6,0,0,595,22361,0,0,929,54841,0,0,240,4714,0,0;QS=3,0;MQSB=0.948436;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,3,36:1:0 0,12,126:4:0 17 2425 . C 0 . DP=18;I16=10,7,0,0,596,21716,0,0,989,58441,0,0,260,5074,0,0;QS=3,0;MQSB=0.943335;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,6,45:2:0 0,12,114:4:0 17 2426 . G 0 . DP=18;I16=10,7,0,0,550,18088,0,0,989,58441,0,0,263,5171,0,0;QS=3,0;MQSB=0.943335;MQ0F=0 PL:DP:DV 0,33,232:11:0 0,6,59:2:0 0,12,113:4:0 17 2427 . T 0 . DP=18;I16=11,6,0,0,618,22654,0,0,958,55682,0,0,275,5403,0,0;QS=3,0;MQSB=0.833753;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,62:2:0 0,9,99:3:0 17 2428 . G 0 . DP=18;I16=11,7,0,0,649,24633,0,0,1018,59282,0,0,281,5535,0,0;QS=3,0;MQSB=0.817948;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,48:2:0 0,12,128:4:0 17 2429 . G 0 . DP=18;I16=10,7,0,0,553,19057,0,0,989,58441,0,0,269,5459,0,0;QS=3,0;MQSB=0.943335;MQ0F=0 PL:DP:DV 0,33,241:11:0 0,6,55:2:0 0,12,117:4:0 17 2430 . T A, 0 . DP=18;I16=10,7,1,0,552,18556,21,441,989,58441,29,841,271,5603,16,256;QS=2.94278,0.0572207,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.817948;BQB=1;MQ0F=0 PL:DP:DV 0,15,212,33,215,222:12:1 0,6,60,6,60,60:2:0 0,12,125,12,125,125:4:0 17 2431 . G 0 . DP=18;I16=11,5,0,0,580,21766,0,0,898,52082,0,0,268,5764,0,0;QS=3,0;MQSB=0.851779;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,3,37:1:0 0,9,98:3:0 17 2432 . G 0 . DP=17;I16=10,7,0,0,593,21479,0,0,958,55682,0,0,295,6179,0,0;QS=3,0;MQSB=0.79189;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,53:2:0 0,9,97:3:0 17 2433 . T 0 . DP=17;I16=10,7,0,0,577,20425,0,0,958,55682,0,0,299,6321,0,0;QS=3,0;MQSB=0.79189;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,57:2:0 0,9,94:3:0 17 2434 . G 0 . DP=17;I16=10,6,0,0,546,19340,0,0,929,54841,0,0,284,6082,0,0;QS=3,0;MQSB=0.948436;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,46:2:0 0,6,62:2:0 17 2435 . C 0 . DP=18;I16=12,5,0,0,597,21843,0,0,958,55682,0,0,304,6626,0,0;QS=3,0;MQSB=0.870325;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,6,58:2:0 0,6,60:2:0 17 2436 . A 0 . DP=18;I16=11,6,0,0,545,18599,0,0,989,58441,0,0,295,6379,0,0;QS=3,0;MQSB=0.955563;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,64:3:0 0,6,61:2:0 17 2437 . C 0 . DP=18;I16=11,5,0,0,573,21195,0,0,929,54841,0,0,297,6541,0,0;QS=3,0;MQSB=0.960687;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,63:2:0 0,6,63:2:0 17 2438 . A 0 . DP=19;I16=12,6,0,0,583,20111,0,0,1018,59282,0,0,328,7322,0,0;QS=3,0;MQSB=0.85394;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,65:2:0 0,6,65:2:0 17 2439 . C 0 . DP=19;I16=11,7,0,0,635,22997,0,0,1049,62041,0,0,314,6974,0,0;QS=3,0;MQSB=0.951002;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,91:3:0 0,6,57:2:0 17 2440 . C 0 . DP=21;I16=13,7,0,0,701,26195,0,0,1138,66482,0,0,346,7840,0,0;QS=3,0;MQSB=0.857404;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,114:4:0 0,6,66:2:0 17 2441 . T 0 . DP=21;I16=13,8,0,0,788,29910,0,0,1198,70082,0,0,360,8068,0,0;QS=3,0;MQSB=0.845496;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,164:5:0 0,6,67:2:0 17 2442 . G 0 . DP=20;I16=12,7,0,0,695,25957,0,0,1109,65641,0,0,342,7596,0,0;QS=3,0;MQSB=0.957193;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,128:4:0 0,6,66:2:0 17 2443 . T 0 . DP=20;I16=13,7,0,0,697,24685,0,0,1138,66482,0,0,373,8345,0,0;QS=3,0;MQSB=0.857404;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,130:4:0 0,6,64:2:0 17 2444 . A 0 . DP=21;I16=13,8,0,0,755,27981,0,0,1198,70082,0,0,379,8489,0,0;QS=3,0;MQSB=0.845496;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,140:4:0 0,6,64:2:0 17 2445 . G 0 . DP=21;I16=12,8,0,0,731,27427,0,0,1169,69241,0,0,359,7927,0,0;QS=3,0;MQSB=0.953497;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,130:4:0 0,6,65:2:0 17 2446 . T 0 . DP=21;I16=13,8,0,0,723,25707,0,0,1198,70082,0,0,389,8633,0,0;QS=3,0;MQSB=0.845496;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,128:4:0 0,6,65:2:0 17 2447 . C 0 . DP=22;I16=14,8,0,0,770,27950,0,0,1258,73682,0,0,394,8732,0,0;QS=3,0;MQSB=0.86151;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,127:4:0 0,6,64:2:0 17 2448 . C 0 . DP=23;I16=14,7,0,0,727,26165,0,0,1167,67323,0,0,388,8674,0,0;QS=3,0;MQSB=0.735784;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,129:5:0 0,6,66:2:0 17 2449 . C 0 . DP=23;I16=13,8,0,0,727,26415,0,0,1198,70082,0,0,363,7787,0,0;QS=3,0;MQSB=0.845496;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,128:5:0 0,6,62:2:0 17 2450 . A 0 . DP=22;I16=12,8,0,0,673,24267,0,0,1138,66482,0,0,371,7959,0,0;QS=3,0;MQSB=0.826565;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,143:5:0 0,6,65:2:0 17 2451 . G 0 . DP=22;I16=14,8,0,0,821,31595,0,0,1227,70923,0,0,429,9401,0,0;QS=3,0;MQSB=0.715049;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,151:5:0 0,6,67:2:0 17 2452 . C 0 . DP=22;I16=14,8,0,0,743,26557,0,0,1227,70923,0,0,437,9613,0,0;QS=3,0;MQSB=0.715049;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,137:5:0 0,6,66:2:0 17 2453 . T 0 . DP=22;I16=14,8,0,0,775,28215,0,0,1227,70923,0,0,445,9845,0,0;QS=3,0;MQSB=0.715049;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,143:5:0 0,6,68:2:0 17 2454 . A 0 . DP=22;I16=14,8,0,0,721,24545,0,0,1227,70923,0,0,453,10097,0,0;QS=3,0;MQSB=0.715049;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,145:5:0 0,6,64:2:0 17 2455 . C 0 . DP=22;I16=14,8,0,0,776,28212,0,0,1227,70923,0,0,461,10369,0,0;QS=3,0;MQSB=0.715049;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,138:5:0 0,6,65:2:0 17 2456 . T 0 . DP=24;I16=14,9,0,0,834,30902,0,0,1318,77282,0,0,444,10036,0,0;QS=3,0;MQSB=0.851535;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,168:6:0 0,6,60:2:0 17 2457 . C 0 . DP=24;I16=15,9,0,0,816,29244,0,0,1347,78123,0,0,478,10924,0,0;QS=3,0;MQSB=0.72325;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,159:6:0 0,6,65:2:0 17 2458 . A 0 . DP=24;I16=15,9,0,0,869,32555,0,0,1347,78123,0,0,487,11209,0,0;QS=3,0;MQSB=0.72325;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,166:6:0 0,6,65:2:0 17 2459 . G T, 0 . DP=24;I16=13,9,1,0,822,31186,13,169,1289,76441,29,841,453,10551,17,289;QS=2.92973,0.0702703,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.851535;BQB=1;MQ0F=0 PL:DP:DV 0,45,255,45,255,255:15:0 0,5,138,15,141,144:6:1 0,6,64,6,64,64:2:0 17 2460 . G 0 . DP=24;I16=14,9,0,0,834,31432,0,0,1318,77282,0,0,477,11065,0,0;QS=3,0;MQSB=0.851535;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,160:6:0 0,6,65:2:0 17 2461 . A G, 0 . DP=24;I16=14,9,1,0,839,31487,13,169,1318,77282,29,841,489,11521,19,361;QS=2.93401,0.0659898,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.72325;BQB=1;MQ0F=0 PL:DP:DV 0,48,255,48,255,255:16:0 0,5,148,15,151,154:6:1 0,6,67,6,67,67:2:0 17 2462 . G 0 . DP=25;I16=15,10,0,0,893,33155,0,0,1407,81723,0,0,514,12090,0,0;QS=3,0;MQSB=0.707404;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,165:6:0 0,6,66:2:0 17 2463 . G T, 0 . DP=25;I16=12,10,1,0,777,28525,14,196,1289,76441,29,841,452,10720,25,625;QS=2.975,0.025,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.825053;BQB=1;MQ0F=0 PL:DP:DV 0,36,255,48,255,255:17:1 0,12,133,12,133,133:4:0 0,6,68,6,68,68:2:0 17 2464 . C 0 . DP=25;I16=15,10,0,0,904,34194,0,0,1407,81723,0,0,526,12458,0,0;QS=3,0;MQSB=0.707404;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,149:6:0 0,6,67:2:0 17 2465 . T 0 . DP=25;I16=14,10,0,0,857,32235,0,0,1347,78123,0,0,506,11994,0,0;QS=3,0;MQSB=0.679965;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,163:6:0 0,6,69:2:0 17 2466 . G 0 . DP=24;I16=14,9,0,0,852,32336,0,0,1318,77282,0,0,509,12025,0,0;QS=3,0;MQSB=0.851535;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,157:6:0 0,6,63:2:0 17 2467 . A 0 . DP=24;I16=14,9,0,0,829,30725,0,0,1318,77282,0,0,513,12121,0,0;QS=3,0;MQSB=0.851535;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,168:6:0 0,6,67:2:0 17 2468 . G 0 . DP=24;I16=15,9,0,0,890,34034,0,0,1347,78123,0,0,541,12807,0,0;QS=3,0;MQSB=0.72325;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,169:6:0 0,6,64:2:0 17 2469 . G 0 . DP=24;I16=15,9,0,0,865,32303,0,0,1347,78123,0,0,544,12882,0,0;QS=3,0;MQSB=0.72325;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,174:6:0 0,6,58:2:0 17 2470 . G 0 . DP=24;I16=14,9,0,0,829,30785,0,0,1318,77282,0,0,521,12295,0,0;QS=3,0;MQSB=0.851535;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,178:6:0 0,6,58:2:0 17 2471 . G 0 . DP=24;I16=14,9,0,0,833,31429,0,0,1318,77282,0,0,523,12345,0,0;QS=3,0;MQSB=0.851535;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,170:5:0 0,6,64:2:0 17 2472 . G 0 . DP=24;I16=13,9,0,0,774,28428,0,0,1289,76441,0,0,499,11733,0,0;QS=3,0;MQSB=0.955854;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,154:5:0 0,6,56:2:0 17 2473 . A 0 . DP=24;I16=13,9,0,0,775,28137,0,0,1258,73682,0,0,508,12078,0,0;QS=3,0;MQSB=0.834768;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,160:5:0 0,6,63:2:0 17 2474 . A 0 . DP=25;I16=15,9,0,0,851,31665,0,0,1378,80882,0,0,524,12322,0,0;QS=3,0;MQSB=0.865888;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,160:5:0 0,9,80:3:0 17 2475 . G 0 . DP=25;I16=15,9,0,0,913,35239,0,0,1378,80882,0,0,525,12323,0,0;QS=3,0;MQSB=0.865888;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,167:5:0 0,9,84:3:0 17 2476 . G 0 . DP=25;I16=13,9,0,0,776,28584,0,0,1289,76441,0,0,488,11544,0,0;QS=3,0;MQSB=0.955854;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,138:5:0 0,9,82:3:0 17 2477 . A 0 . DP=25;I16=14,9,0,0,867,34329,0,0,1318,77282,0,0,515,12223,0,0;QS=3,0;MQSB=0.851535;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,178:5:0 0,9,91:3:0 17 2478 . C 0 . DP=25;I16=14,9,0,0,897,36911,0,0,1318,77282,0,0,517,12289,0,0;QS=3,0;MQSB=0.851535;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,175:5:0 0,9,87:3:0 17 2479 . T 0 . DP=25;I16=15,9,0,0,921,37957,0,0,1378,80882,0,0,529,12467,0,0;QS=3,0;MQSB=0.865888;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,176:5:0 0,9,97:3:0 17 2480 . G 0 . DP=25;I16=14,9,0,0,877,35485,0,0,1349,80041,0,0,504,11864,0,0;QS=3,0;MQSB=0.960618;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,176:5:0 0,9,88:3:0 17 2481 . C 0 . DP=25;I16=13,9,0,0,848,34542,0,0,1289,76441,0,0,496,11838,0,0;QS=3,0;MQSB=0.955854;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,183:5:0 0,9,88:3:0 17 2482 . T 0 . DP=25;I16=15,9,0,0,905,37331,0,0,1378,80882,0,0,526,12430,0,0;QS=3,0;MQSB=0.865888;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,178:5:0 0,9,96:3:0 17 2483 . T 0 . DP=25;I16=14,9,0,0,879,36187,0,0,1318,77282,0,0,517,12311,0,0;QS=3,0;MQSB=0.851535;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,180:5:0 0,9,89:3:0 17 2484 . G 0 . DP=25;I16=14,9,0,0,884,35142,0,0,1349,80041,0,0,494,11604,0,0;QS=3,0;MQSB=0.960618;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,174:5:0 0,9,84:3:0 17 2485 . A T, 0 . DP=25;I16=14,9,1,0,891,36757,17,289,1349,80041,29,841,490,11502,25,625;QS=2.96926,0.0307414,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.865888;BQB=1;MQ0F=0 PL:DP:DV 0,31,255,45,255,255:16:1 0,15,193,15,193,193:5:0 0,9,93,9,93,93:3:0 17 2486 . G 0 . DP=25;I16=15,9,0,0,898,36230,0,0,1378,80882,0,0,511,12041,0,0;QS=3,0;MQSB=0.865888;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,153:5:0 0,9,90:3:0 17 2487 . C 0 . DP=25;I16=14,9,0,0,793,30695,0,0,1349,80041,0,0,482,11346,0,0;QS=3,0;MQSB=0.960618;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,175:5:0 0,9,91:3:0 17 2488 . C 0 . DP=25;I16=13,9,0,0,841,34597,0,0,1289,76441,0,0,457,10841,0,0;QS=3,0;MQSB=0.955854;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,174:5:0 0,9,93:3:0 17 2489 . C 0 . DP=23;I16=11,9,0,0,801,34227,0,0,1169,69241,0,0,454,10788,0,0;QS=3,0;MQSB=0.943233;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,187:5:0 0,9,93:3:0 17 2490 . A 0 . DP=23;I16=11,10,0,0,818,34642,0,0,1229,72841,0,0,451,10695,0,0;QS=3,0;MQSB=0.939898;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,175:5:0 0,12,128:4:0 17 2491 . G 0 . DP=23;I16=12,10,0,0,845,35143,0,0,1258,73682,0,0,471,11097,0,0;QS=3,0;MQSB=0.804615;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,178:5:0 0,12,121:4:0 17 2492 . G A, 0 . DP=23;I16=11,10,1,0,845,36207,16,256,1229,72841,29,841,446,10494,21,441;QS=2.96708,0.0329218,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.804615;BQB=1;MQ0F=0 PL:DP:DV 0,22,255,36,255,255:13:1 0,15,187,15,187,187:5:0 0,12,124,12,124,124:4:0 17 2493 . A G, 0 . DP=23;I16=11,10,1,0,855,37007,13,169,1229,72841,29,841,442,10340,20,400;QS=2.97269,0.0273109,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.804615;BQB=1;MQ0F=0 PL:DP:DV 0,25,255,36,255,255:13:1 0,15,191,15,191,191:5:0 0,12,127,12,127,127:4:0 17 2494 . G 0 . DP=23;I16=11,10,0,0,802,32720,0,0,1229,72841,0,0,437,10153,0,0;QS=3,0;MQSB=0.939898;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,179:5:0 0,12,118:4:0 17 2495 . T 0 . DP=23;I16=10,10,0,0,737,29861,0,0,1138,66482,0,0,413,9513,0,0;QS=3,0;MQSB=0.751477;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,172:5:0 0,12,113:4:0 17 2496 . T 0 . DP=23;I16=12,10,0,0,815,32793,0,0,1258,73682,0,0,442,10026,0,0;QS=3,0;MQSB=0.804615;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,183:5:0 0,12,123:4:0 17 2497 . T 0 . DP=22;I16=12,9,0,0,807,33723,0,0,1198,70082,0,0,436,9814,0,0;QS=3,0;MQSB=0.815018;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,177:5:0 0,12,131:4:0 17 2498 . G 0 . DP=22;I16=12,9,0,0,808,33264,0,0,1198,70082,0,0,430,9622,0,0;QS=3,0;MQSB=0.815018;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,173:5:0 0,12,127:4:0 17 2499 . A 0 . DP=22;I16=12,9,0,0,847,36803,0,0,1198,70082,0,0,424,9450,0,0;QS=3,0;MQSB=0.815018;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,185:5:0 0,12,133:4:0 17 2500 . G 0 . DP=22;I16=11,9,0,0,772,32546,0,0,1169,69241,0,0,404,9078,0,0;QS=3,0;MQSB=0.943233;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,168:5:0 0,12,129:4:0 17 2501 . G 0 . DP=22;I16=11,8,0,0,768,33120,0,0,1109,65641,0,0,373,8293,0,0;QS=3,0;MQSB=0.946915;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,146:4:0 0,12,118:4:0 17 2502 . C 0 . DP=22;I16=12,8,0,0,798,33902,0,0,1138,66482,0,0,378,8270,0,0;QS=3,0;MQSB=0.826565;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,155:4:0 0,12,125:4:0 17 2503 . T 0 . DP=22;I16=12,9,0,0,851,36841,0,0,1198,70082,0,0,396,8746,0,0;QS=3,0;MQSB=0.815018;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,180:5:0 0,12,139:4:0 17 2504 . G 0 . DP=22;I16=12,9,0,0,787,31955,0,0,1198,70082,0,0,389,8615,0,0;QS=3,0;MQSB=0.815018;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,171:5:0 0,12,129:4:0 17 2505 . C 0 . DP=21;I16=11,9,0,0,792,33440,0,0,1138,66482,0,0,383,8501,0,0;QS=3,0;MQSB=0.791547;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,169:5:0 0,12,132:4:0 17 2506 . T 0 . DP=22;I16=11,10,0,0,798,32868,0,0,1198,70082,0,0,376,8354,0,0;QS=3,0;MQSB=0.780412;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,175:5:0 0,15,169:5:0 17 2507 . G 0 . DP=21;I16=9,10,0,0,716,30074,0,0,1109,65641,0,0,365,8189,0,0;QS=3,0;MQSB=0.920044;MQ0F=0 PL:DP:DV 0,27,241:9:0 0,15,163:5:0 0,15,153:5:0 17 2508 . T 0 . DP=21;I16=9,10,0,0,724,30396,0,0,1109,65641,0,0,361,8089,0,0;QS=3,0;MQSB=0.920044;MQ0F=0 PL:DP:DV 0,27,241:9:0 0,15,158:5:0 0,15,162:5:0 17 2509 . G T, 0 . DP=21;I16=8,10,1,0,710,30408,35,1225,1049,62041,60,3600,330,7282,25,625;QS=2.80663,0.19337,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.920044;BQB=1;MQ0F=0 PL:DP:DV 0,27,253,27,253,253:9:0 20,0,122,32,125,150:5:1 0,15,163,15,163,163:5:0 17 2510 . A 0 . DP=21;I16=10,10,0,0,778,33128,0,0,1138,66482,0,0,352,7754,0,0;QS=3,0;MQSB=0.751477;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,174:5:0 0,15,161:5:0 17 2511 . G 0 . DP=21;I16=10,10,0,0,771,32165,0,0,1138,66482,0,0,344,7558,0,0;QS=3,0;MQSB=0.751477;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,161:5:0 0,15,159:5:0 17 2512 . C 0 . DP=21;I16=10,10,0,0,790,33406,0,0,1138,66482,0,0,336,7386,0,0;QS=3,0;MQSB=0.751477;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,175:5:0 0,15,160:5:0 17 2513 . T 0 . DP=21;I16=10,10,0,0,755,31503,0,0,1138,66482,0,0,327,7189,0,0;QS=3,0;MQSB=0.751477;MQ0F=0 PL:DP:DV 0,30,246:10:0 0,15,180:5:0 0,15,156:5:0 17 2514 . G 0 . DP=20;I16=9,10,0,0,702,28002,0,0,1109,65641,0,0,319,7017,0,0;QS=3,0;MQSB=0.920044;MQ0F=0 PL:DP:DV 0,27,228:9:0 0,15,171:5:0 0,15,155:5:0 17 2515 . T 0 . DP=19;I16=8,10,0,0,642,25888,0,0,1049,62041,0,0,312,6868,0,0;QS=3,0;MQSB=0.906029;MQ0F=0 PL:DP:DV 0,24,182:8:0 0,15,172:5:0 0,15,141:5:0 17 2516 . G 0 . DP=19;I16=8,10,0,0,685,28155,0,0,1049,62041,0,0,303,6641,0,0;QS=3,0;MQSB=0.906029;MQ0F=0 PL:DP:DV 0,24,211:8:0 0,15,175:5:0 0,15,147:5:0 17 2517 . A 0 . DP=18;I16=8,9,0,0,660,27600,0,0,989,58441,0,0,295,6435,0,0;QS=3,0;MQSB=0.91051;MQ0F=0 PL:DP:DV 0,24,215:8:0 0,12,139:4:0 0,15,161:5:0 17 2518 . T 0 . DP=17;I16=6,9,0,0,611,26911,0,0,900,54000,0,0,273,6023,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,188:7:0 0,12,144:4:0 0,12,146:4:0 17 2519 . C 0 . DP=16;I16=7,8,0,0,531,20111,0,0,900,54000,0,0,282,6078,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,175:7:0 0,12,141:4:0 0,12,125:4:0 17 2520 . G 0 . DP=15;I16=6,8,0,0,501,19731,0,0,840,50400,0,0,277,5923,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,187:7:0 0,12,129:4:0 0,9,101:3:0 17 2521 . C 0 . DP=15;I16=6,8,0,0,583,25777,0,0,840,50400,0,0,272,5782,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,214:7:0 0,12,149:4:0 0,9,111:3:0 17 2522 . A 0 . DP=16;I16=6,8,0,0,516,20930,0,0,840,50400,0,0,266,5606,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,173:7:0 0,12,139:4:0 0,9,114:3:0 17 2523 . T 0 . DP=16;I16=6,9,0,0,588,25538,0,0,900,54000,0,0,270,5546,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,207:7:0 0,15,157:5:0 0,9,117:3:0 17 2524 . C 0 . DP=16;I16=5,9,0,0,548,23502,0,0,840,50400,0,0,256,5342,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,182:6:0 0,15,156:5:0 0,9,112:3:0 17 2525 . A C, 0 . DP=17;I16=6,9,0,1,550,23138,28,784,900,54000,60,3600,248,5174,12,144;QS=2.86,0.14,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0 PL:DP:DV 0,24,168,24,168,168:8:0 13,0,138,25,141,159:5:1 0,9,117,9,117,117:3:0 17 2526 . C 0 . DP=18;I16=6,11,0,0,677,28649,0,0,1020,61200,0,0,256,5232,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,229:9:0 0,15,183:5:0 0,9,113:3:0 17 2527 . T 0 . DP=18;I16=6,11,0,0,674,29286,0,0,1020,61200,0,0,252,5118,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,217:9:0 0,15,183:5:0 0,9,119:3:0 17 2528 . G 0 . DP=18;I16=6,11,0,0,701,30729,0,0,1020,61200,0,0,248,5028,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,234:9:0 0,15,184:5:0 0,9,119:3:0 17 2529 . C 0 . DP=18;I16=6,11,0,0,676,28974,0,0,1020,61200,0,0,244,4962,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,228:9:0 0,15,175:5:0 0,9,117:3:0 17 2530 . A 0 . DP=18;I16=6,10,0,0,624,26620,0,0,960,57600,0,0,223,4631,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,209:9:0 0,12,153:4:0 0,9,116:3:0 17 2531 . T 0 . DP=17;I16=6,10,0,0,641,27911,0,0,960,57600,0,0,236,4852,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,210:8:0 0,15,175:5:0 0,9,122:3:0 17 2532 . T 0 . DP=17;I16=6,10,0,0,634,27460,0,0,960,57600,0,0,230,4708,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,212:8:0 0,15,173:5:0 0,9,117:3:0 17 2533 . C 0 . DP=18;I16=6,11,0,0,614,24196,0,0,1020,61200,0,0,224,4588,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,212:9:0 0,15,159:5:0 0,9,104:3:0 17 2534 . C 0 . DP=16;I16=5,10,0,0,595,25141,0,0,900,54000,0,0,221,4491,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,202:8:0 0,12,144:4:0 0,9,110:3:0 17 2535 . A 0 . DP=16;I16=5,10,0,0,617,27711,0,0,900,54000,0,0,218,4416,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,204:8:0 0,12,136:4:0 0,9,123:3:0 17 2536 . G 0 . DP=15;I16=4,10,0,0,543,23023,0,0,840,50400,0,0,216,4362,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,195:8:0 0,9,102:3:0 0,9,117:3:0 17 2537 . C 0 . DP=15;I16=4,10,0,0,555,24075,0,0,840,50400,0,0,213,4279,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,195:8:0 0,9,113:3:0 0,9,115:3:0 17 2538 . C 0 . DP=15;I16=5,9,0,0,510,21070,0,0,840,50400,0,0,211,4217,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,157:7:0 0,9,129:3:0 0,12,126:4:0 17 2539 . C 0 . DP=15;I16=5,9,0,0,464,16896,0,0,840,50400,0,0,209,4125,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,146:7:0 0,9,122:3:0 0,12,111:4:0 17 2540 . G 0 . DP=16;I16=6,9,0,0,548,21860,0,0,900,54000,0,0,207,4053,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,189:7:0 0,12,139:4:0 0,12,110:4:0 17 2541 . G 0 . DP=15;I16=5,9,0,0,535,22527,0,0,840,50400,0,0,207,4001,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,170:7:0 0,9,117:3:0 0,12,140:4:0 17 2542 . T 0 . DP=15;I16=4,9,0,0,527,23207,0,0,780,46800,0,0,203,3953,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,183:7:0 0,9,125:3:0 0,9,109:3:0 17 2543 . G 0 . DP=15;I16=5,9,0,0,540,23004,0,0,840,50400,0,0,207,3957,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,196:7:0 0,9,117:3:0 0,12,117:4:0 17 2544 . A 0 . DP=16;I16=6,9,0,0,569,24139,0,0,900,54000,0,0,207,3965,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,202:8:0 0,9,119:3:0 0,12,132:4:0 17 2545 . C 0 . DP=16;I16=6,9,0,0,583,24657,0,0,900,54000,0,0,208,3994,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,217:8:0 0,9,118:3:0 0,12,130:4:0 17 2546 . A 0 . DP=16;I16=6,9,0,0,608,27290,0,0,900,54000,0,0,209,4045,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,206:8:0 0,9,133:3:0 0,12,144:4:0 17 2547 . G 0 . DP=16;I16=6,9,0,0,597,26215,0,0,900,54000,0,0,211,4117,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,191:7:0 0,9,131:3:0 0,15,150:5:0 17 2548 . A 0 . DP=16;I16=6,9,0,0,632,28806,0,0,900,54000,0,0,214,4210,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,196:7:0 0,9,131:3:0 0,15,171:5:0 17 2549 . G 0 . DP=16;I16=6,9,0,0,594,25254,0,0,900,54000,0,0,217,4325,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,180:7:0 0,9,132:3:0 0,15,160:5:0 17 2550 . T 0 . DP=16;I16=6,9,0,0,572,24134,0,0,900,54000,0,0,219,4413,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,182:7:0 0,9,122:3:0 0,15,148:5:0 17 2551 . G 0 . DP=16;I16=6,9,0,0,578,24606,0,0,900,54000,0,0,220,4474,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,181:7:0 0,9,131:3:0 0,15,151:5:0 17 2552 . A 0 . DP=15;I16=6,8,0,0,585,26419,0,0,840,50400,0,0,221,4505,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,178:6:0 0,9,129:3:0 0,15,168:5:0 17 2553 . G 0 . DP=15;I16=6,8,0,0,528,21944,0,0,840,50400,0,0,222,4554,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,170:6:0 0,9,117:3:0 0,15,142:5:0 17 2554 . T 0 . DP=15;I16=6,8,0,0,543,23015,0,0,840,50400,0,0,223,4621,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,156:6:0 0,9,129:3:0 0,15,160:5:0 17 2555 . C 0 . DP=15;I16=6,8,0,0,566,24416,0,0,840,50400,0,0,224,4706,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,173:6:0 0,9,131:3:0 0,15,159:5:0 17 2556 . A 0 . DP=14;I16=6,7,0,0,487,20095,0,0,780,46800,0,0,226,4808,0,0;QS=3,0;MQSB=0.961166;MQ0F=0 PL:DP:DV 0,15,141:5:0 0,9,125:3:0 0,15,146:5:0 17 2557 . C 0 . DP=13;I16=5,8,0,0,471,17481,0,0,780,46800,0,0,249,5325,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,15,161:5:0 0,9,96:3:0 0,15,151:5:0 17 2558 . T 0 . DP=14;I16=5,9,0,0,535,20597,0,0,840,50400,0,0,251,5417,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,173:6:0 0,9,99:3:0 0,15,174:5:0 17 2559 . G 0 . DP=14;I16=5,9,0,0,509,18693,0,0,840,50400,0,0,253,5475,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,172:6:0 0,9,98:3:0 0,15,156:5:0 17 2560 . T 0 . DP=14;I16=5,9,0,0,489,17587,0,0,840,50400,0,0,255,5549,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,160:6:0 0,9,106:3:0 0,15,144:5:0 17 2561 . C 0 . DP=14;I16=5,9,0,0,489,17477,0,0,840,50400,0,0,257,5639,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,164:6:0 0,9,96:3:0 0,15,153:5:0 17 2562 . T 0 . DP=13;I16=5,8,0,0,460,17016,0,0,780,46800,0,0,260,5744,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,15,139:5:0 0,9,99:3:0 0,15,167:5:0 17 2563 . C 0 . DP=14;I16=5,8,0,0,433,15133,0,0,780,46800,0,0,263,5863,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,15,149:5:0 0,9,83:3:0 0,15,142:5:0 17 2564 . A G, 0 . DP=15;I16=1,4,4,5,174,6124,316,11352,300,18000,540,32400,61,1311,184,4034;QS=0.988263,2.01174,0;VDB=0.690812;SGB=-3.20711;RPB=0.197899;MQB=1;MQSB=1;BQB=0.965069;MQ0F=0 PL:DP:DV 88,0,78,98,87,171:6:3 57,0,56,63,62,117:4:2 124,12,0,124,12,124:4:4 17 2565 . A 0 . DP=15;I16=6,9,0,0,562,21216,0,0,900,54000,0,0,274,6076,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,178:6:0 0,12,134:4:0 0,15,166:5:0 17 2566 . A 0 . DP=15;I16=6,9,0,0,545,20019,0,0,900,54000,0,0,276,6098,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,181:6:0 0,12,120:4:0 0,15,162:5:0 17 2567 . A 0 . DP=16;I16=7,9,0,0,580,21342,0,0,960,57600,0,0,278,6136,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,177:6:0 0,15,151:5:0 0,15,166:5:0 17 2568 . A 0 . DP=16;I16=7,8,0,0,555,20795,0,0,900,54000,0,0,256,5566,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,180:6:0 0,12,132:4:0 0,15,167:5:0 17 2569 . A 0 . DP=17;I16=7,10,0,0,661,25943,0,0,1020,61200,0,0,284,6264,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,213:7:0 0,15,163:5:0 0,15,172:5:0 17 2570 . G 0 . DP=18;I16=8,10,0,0,664,24968,0,0,1080,64800,0,0,287,6305,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,213:7:0 0,15,152:5:0 0,18,176:6:0 17 2571 . A 0 . DP=18;I16=8,10,0,0,618,21870,0,0,1080,64800,0,0,291,6365,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,195:7:0 0,15,155:5:0 0,18,155:6:0 17 2572 . A 0 . DP=18;I16=8,10,0,0,631,22829,0,0,1080,64800,0,0,295,6445,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,194:7:0 0,15,150:5:0 0,18,173:6:0 17 2573 . A 0 . DP=18;I16=8,10,0,0,690,26830,0,0,1080,64800,0,0,298,6494,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,210:7:0 0,15,170:5:0 0,18,183:6:0 17 2574 . G 0 . DP=18;I16=8,10,0,0,664,24884,0,0,1080,64800,0,0,301,6561,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,211:7:0 0,15,152:5:0 0,18,176:6:0 17 2575 . G 0 . DP=18;I16=8,10,0,0,642,23904,0,0,1080,64800,0,0,305,6645,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,242:8:0 0,15,145:5:0 0,15,140:5:0 17 2576 . A 0 . DP=18;I16=7,10,0,0,595,21311,0,0,1020,61200,0,0,285,6121,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,210:8:0 0,15,153:5:0 0,12,131:4:0 17 2577 . A 0 . DP=19;I16=8,10,0,0,682,26398,0,0,1080,64800,0,0,290,6240,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,244:8:0 0,15,161:5:0 0,15,155:5:0 17 2578 . G 0 . DP=18;I16=9,9,0,0,643,24115,0,0,1080,64800,0,0,322,7002,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,249:8:0 0,12,114:4:0 0,18,161:6:0 17 2579 . A 0 . DP=18;I16=9,8,0,0,634,23870,0,0,1020,61200,0,0,304,6532,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,229:8:0 0,9,110:3:0 0,18,176:6:0 17 2580 . A 0 . DP=18;I16=9,9,0,0,629,22593,0,0,1080,64800,0,0,336,7330,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,223:8:0 0,12,117:4:0 0,18,169:6:0 17 2581 . A 0 . DP=19;I16=10,9,0,0,691,25669,0,0,1140,68400,0,0,343,7521,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,12,133:4:0 0,18,166:6:0 17 2582 . T 0 . DP=19;I16=10,9,0,0,674,24218,0,0,1140,68400,0,0,350,7682,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,235:9:0 0,12,138:4:0 0,18,168:6:0 17 2583 . A 0 . DP=19;I16=10,9,0,0,693,25465,0,0,1140,68400,0,0,357,7865,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,247:9:0 0,12,126:4:0 0,18,178:6:0 17 2584 . A 0 . DP=19;I16=10,9,0,0,702,26340,0,0,1140,68400,0,0,363,8019,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,243:9:0 0,12,134:4:0 0,18,187:6:0 17 2585 . A 0 . DP=19;I16=9,9,0,0,690,26700,0,0,1080,64800,0,0,350,7818,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,250:9:0 0,9,114:3:0 0,18,190:6:0 17 2586 . G 0 . DP=19;I16=10,9,0,0,714,27314,0,0,1140,68400,0,0,373,8283,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,12,127:4:0 0,18,176:6:0 17 2587 . A 0 . DP=20;I16=9,9,0,0,665,24781,0,0,1049,62041,0,0,343,7717,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,137:4:0 0,12,136:4:0 17 2588 . A 0 . DP=21;I16=12,9,0,0,737,26531,0,0,1229,72841,0,0,384,8620,0,0;QS=3,0;MQSB=0.950149;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,138:4:0 0,18,162:6:0 17 2589 . A 0 . DP=22;I16=13,9,0,0,780,28412,0,0,1289,76441,0,0,390,8770,0,0;QS=3,0;MQSB=0.955854;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,142:4:0 0,18,170:6:0 17 2590 . A 0 . DP=22;I16=13,9,0,0,774,28352,0,0,1289,76441,0,0,396,8894,0,0;QS=3,0;MQSB=0.955854;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,135:4:0 0,18,160:6:0 17 2591 . C 0 . DP=21;I16=13,8,0,0,765,28617,0,0,1229,72841,0,0,403,9041,0,0;QS=3,0;MQSB=0.95891;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,136:4:0 0,15,136:5:0 17 2592 . A 0 . DP=21;I16=13,8,0,0,763,28325,0,0,1229,72841,0,0,410,9210,0,0;QS=3,0;MQSB=0.95891;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,140:4:0 0,15,139:5:0 17 2593 . A 0 . DP=21;I16=13,8,0,0,754,27888,0,0,1229,72841,0,0,416,9350,0,0;QS=3,0;MQSB=0.95891;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,141:4:0 0,15,147:5:0 17 2594 . A 0 . DP=21;I16=13,8,0,0,775,29179,0,0,1229,72841,0,0,422,9510,0,0;QS=3,0;MQSB=0.95891;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,130:4:0 0,15,153:5:0 17 2595 . T 0 . DP=22;I16=14,8,0,0,766,27472,0,0,1289,76441,0,0,427,9639,0,0;QS=3,0;MQSB=0.963419;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,119:4:0 0,15,143:5:0 17 2596 . A 0 . DP=22;I16=13,8,0,0,751,27409,0,0,1229,72841,0,0,407,9111,0,0;QS=3,0;MQSB=0.95891;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,123:4:0 0,12,129:4:0 17 2597 . A 0 . DP=22;I16=14,8,0,0,811,30147,0,0,1289,76441,0,0,437,9851,0,0;QS=3,0;MQSB=0.963419;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,145:4:0 0,15,145:5:0 17 2598 . A 0 . DP=22;I16=14,8,0,0,817,30915,0,0,1289,76441,0,0,442,9984,0,0;QS=3,0;MQSB=0.963419;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,136:4:0 0,15,159:5:0 17 2599 . A 0 . DP=22;I16=14,8,0,0,800,29912,0,0,1289,76441,0,0,447,10135,0,0;QS=3,0;MQSB=0.963419;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,126:4:0 0,15,150:5:0 17 2600 . A 0 . DP=22;I16=14,8,0,0,784,29138,0,0,1289,76441,0,0,451,10255,0,0;QS=3,0;MQSB=0.963419;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,113:4:0 0,15,150:5:0 17 2601 . T 0 . DP=22;I16=14,8,0,0,770,27820,0,0,1289,76441,0,0,454,10344,0,0;QS=3,0;MQSB=0.963419;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,118:4:0 0,15,142:5:0 17 2602 . A 0 . DP=23;I16=15,8,0,0,825,29971,0,0,1349,80041,0,0,457,10451,0,0;QS=3,0;MQSB=0.967216;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,143:4:0 0,18,154:6:0 17 2603 . A 0 . DP=23;I16=15,8,0,0,862,32840,0,0,1349,80041,0,0,460,10526,0,0;QS=3,0;MQSB=0.967216;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,138:4:0 0,18,172:6:0 17 2604 . T 0 . DP=23;I16=15,8,0,0,822,29902,0,0,1349,80041,0,0,463,10619,0,0;QS=3,0;MQSB=0.967216;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,129:4:0 0,18,154:6:0 17 2605 . A 0 . DP=23;I16=14,8,0,0,845,32617,0,0,1289,76441,0,0,441,10105,0,0;QS=3,0;MQSB=0.963419;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,143:4:0 0,15,157:5:0 17 2606 . G 0 . DP=23;I16=14,8,0,0,812,30796,0,0,1289,76441,0,0,444,10234,0,0;QS=3,0;MQSB=0.963419;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,129:4:0 0,15,142:5:0 17 2607 . T 0 . DP=22;I16=15,7,0,0,782,28440,0,0,1289,76441,0,0,472,10954,0,0;QS=3,0;MQSB=0.970024;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,119:4:0 0,15,132:5:0 17 2608 . G 0 . DP=22;I16=15,7,0,0,851,33169,0,0,1289,76441,0,0,474,11014,0,0;QS=3,0;MQSB=0.970024;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,132:4:0 0,15,127:5:0 17 2609 . C 0 . DP=22;I16=15,7,0,0,796,29454,0,0,1289,76441,0,0,475,11041,0,0;QS=3,0;MQSB=0.970024;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,137:4:0 0,15,124:5:0 17 2610 . A 0 . DP=22;I16=15,7,0,0,830,31684,0,0,1289,76441,0,0,476,11086,0,0;QS=3,0;MQSB=0.970024;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,146:4:0 0,15,121:5:0 17 2611 . G 0 . DP=22;I16=15,7,0,0,824,32048,0,0,1289,76441,0,0,477,11149,0,0;QS=3,0;MQSB=0.970024;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,129:4:0 0,15,122:5:0 17 2612 . A 0 . DP=22;I16=14,7,0,0,771,29035,0,0,1229,72841,0,0,453,10605,0,0;QS=3,0;MQSB=0.966484;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,130:4:0 0,12,117:4:0 17 2613 . C 0 . DP=22;I16=15,7,0,0,832,31926,0,0,1289,76441,0,0,478,11278,0,0;QS=3,0;MQSB=0.970024;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,138:4:0 0,15,120:5:0 17 2614 . A 0 . DP=21;I16=15,6,0,0,791,30065,0,0,1229,72841,0,0,477,11241,0,0;QS=3,0;MQSB=0.973096;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,110:3:0 0,15,140:5:0 17 2615 . A 0 . DP=21;I16=15,6,0,0,777,29101,0,0,1229,72841,0,0,475,11167,0,0;QS=3,0;MQSB=0.973096;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,99:3:0 0,15,142:5:0 17 2616 . A 0 . DP=21;I16=15,6,0,0,734,26922,0,0,1229,72841,0,0,472,11056,0,0;QS=3,0;MQSB=0.973096;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,90:3:0 0,15,144:5:0 17 2617 . A 0 . DP=21;I16=15,6,0,0,810,31654,0,0,1229,72841,0,0,469,10959,0,0;QS=3,0;MQSB=0.973096;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,95:3:0 0,15,140:5:0 17 2618 . G 0 . DP=23;I16=16,6,0,0,863,34219,0,0,1289,76441,0,0,441,10251,0,0;QS=3,0;MQSB=0.97584;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,74:2:0 0,15,132:5:0 17 2619 . G 0 . DP=23;I16=16,6,0,0,807,30109,0,0,1289,76441,0,0,439,10135,0,0;QS=3,0;MQSB=0.97584;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,66:2:0 0,15,127:5:0 17 2620 . C 0 . DP=23;I16=16,7,0,0,780,28242,0,0,1349,80041,0,0,462,10664,0,0;QS=3,0;MQSB=0.973027;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,87:3:0 0,15,115:5:0 17 2621 . C 0 . DP=23;I16=16,7,0,0,860,32944,0,0,1349,80041,0,0,459,10537,0,0;QS=3,0;MQSB=0.973027;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,112:3:0 0,15,124:5:0 17 2622 . T 0 . DP=24;I16=17,7,0,0,908,35474,0,0,1409,83641,0,0,456,10428,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,115:3:0 0,15,134:5:0 17 2623 . T 0 . DP=24;I16=17,7,0,0,850,30978,0,0,1409,83641,0,0,453,10289,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,114:3:0 0,15,129:5:0 17 2624 . G 0 . DP=24;I16=17,7,0,0,882,33332,0,0,1409,83641,0,0,450,10172,0,0;QS=3,0;MQSB=0.975597;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,101:3:0 0,15,133:5:0 17 2625 . A 0 . DP=23;I16=17,6,0,0,855,32593,0,0,1349,80041,0,0,448,10076,0,0;QS=3,0;MQSB=0.978183;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,114:3:0 0,15,135:5:0 17 2626 . C 0 . DP=23;I16=17,6,0,0,847,31625,0,0,1349,80041,0,0,446,10000,0,0;QS=3,0;MQSB=0.978183;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,100:3:0 0,15,122:5:0 17 2627 . C 0 . DP=23;I16=17,6,0,0,863,32865,0,0,1349,80041,0,0,444,9944,0,0;QS=3,0;MQSB=0.978183;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,104:3:0 0,15,129:5:0 17 2628 . C 0 . DP=24;I16=17,6,0,0,793,28559,0,0,1349,80041,0,0,431,9757,0,0;QS=3,0;MQSB=0.978183;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,95:3:0 0,15,127:5:0 17 2629 . A 0 . DP=24;I16=18,6,0,0,893,33537,0,0,1409,83641,0,0,439,9789,0,0;QS=3,0;MQSB=0.980199;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,101:3:0 0,18,148:6:0 17 2630 . T 0 . DP=24;I16=17,6,0,0,836,31094,0,0,1380,82800,0,0,412,9116,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,112:3:0 0,18,140:6:0 17 2631 . C 0 . DP=24;I16=18,6,0,0,900,34378,0,0,1409,83641,0,0,435,9713,0,0;QS=3,0;MQSB=0.980199;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,104:3:0 0,18,142:6:0 17 2632 . T 0 . DP=24;I16=18,6,0,0,937,37097,0,0,1409,83641,0,0,433,9705,0,0;QS=3,0;MQSB=0.980199;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,113:3:0 0,18,164:6:0 17 2633 . A 0 . DP=23;I16=18,5,0,0,801,28913,0,0,1349,80041,0,0,431,9667,0,0;QS=3,0;MQSB=0.982789;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,95:3:0 0,18,148:6:0 17 2634 . G 0 . DP=23;I16=19,3,0,0,861,34125,0,0,1289,76441,0,0,405,9023,0,0;QS=3,0;MQSB=0.989755;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,74:2:0 0,18,149:6:0 17 2635 . C 0 . DP=23;I16=19,4,0,0,848,32154,0,0,1349,80041,0,0,429,9599,0,0;QS=3,0;MQSB=0.986928;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,107:3:0 0,18,148:6:0 17 2636 . T 0 . DP=24;I16=19,4,0,0,871,33973,0,0,1349,80041,0,0,428,9572,0,0;QS=3,0;MQSB=0.986928;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,98:3:0 0,18,160:6:0 17 2637 . T 0 . DP=24;I16=19,5,0,0,871,32073,0,0,1409,83641,0,0,428,9568,0,0;QS=3,0;MQSB=0.984335;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,111:3:0 0,18,144:6:0 17 2638 . T 0 . DP=24;I16=19,5,0,0,821,28851,0,0,1409,83641,0,0,428,9588,0,0;QS=3,0;MQSB=0.984335;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,103:3:0 0,18,135:6:0 17 2639 . G 0 . DP=24;I16=18,6,0,0,842,30840,0,0,1409,83641,0,0,428,9582,0,0;QS=3,0;MQSB=0.980199;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,106:3:0 0,15,125:5:0 17 2640 . G 0 . DP=24;I16=17,6,0,0,749,25957,0,0,1380,82800,0,0,404,8976,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,84:3:0 0,15,117:5:0 17 2641 . C 0 . DP=23;I16=17,6,0,0,834,31792,0,0,1349,80041,0,0,431,9645,0,0;QS=3,0;MQSB=0.978183;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,61:2:0 0,15,129:5:0 17 2642 . C 0 . DP=23;I16=17,6,0,0,809,30033,0,0,1349,80041,0,0,433,9713,0,0;QS=3,0;MQSB=0.978183;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,58:2:0 0,15,131:5:0 17 2643 . C 0 . DP=23;I16=17,6,0,0,856,33004,0,0,1349,80041,0,0,434,9756,0,0;QS=3,0;MQSB=0.978183;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,57:2:0 0,15,133:5:0 17 2644 . T 0 . DP=22;I16=16,5,0,0,805,31419,0,0,1229,72841,0,0,428,9648,0,0;QS=3,0;MQSB=0.978919;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,73:2:0 0,15,143:5:0 17 2645 . C 0 . DP=22;I16=15,6,0,0,823,32499,0,0,1229,72841,0,0,415,9323,0,0;QS=3,0;MQSB=0.973096;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,78:2:0 0,12,122:4:0 17 2646 . A 0 . DP=22;I16=16,6,0,0,826,31566,0,0,1289,76441,0,0,430,9524,0,0;QS=3,0;MQSB=0.97584;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,79:2:0 0,15,129:5:0 17 2647 . G 0 . DP=22;I16=16,6,0,0,803,30643,0,0,1289,76441,0,0,428,9460,0,0;QS=3,0;MQSB=0.97584;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,77:2:0 0,15,129:5:0 17 2648 . C 0 . DP=21;I16=15,6,0,0,811,31709,0,0,1229,72841,0,0,426,9368,0,0;QS=3,0;MQSB=0.973096;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,80:2:0 0,12,118:4:0 17 2649 . A 0 . DP=23;I16=16,6,0,0,789,29215,0,0,1258,73682,0,0,414,9196,0,0;QS=3,0;MQSB=0.934321;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,93:3:0 0,12,105:4:0 17 2650 . T 0 . DP=23;I16=16,7,0,0,856,32340,0,0,1318,77282,0,0,423,9197,0,0;QS=3,0;MQSB=0.955805;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,103:3:0 0,12,118:4:0 17 2651 . C 0 . DP=23;I16=16,7,0,0,871,33599,0,0,1318,77282,0,0,422,9124,0,0;QS=3,0;MQSB=0.955805;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,95:3:0 0,12,121:4:0 17 2652 . A 0 . DP=23;I16=16,7,0,0,820,30324,0,0,1318,77282,0,0,421,9077,0,0;QS=3,0;MQSB=0.955805;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,87:3:0 0,12,126:4:0 17 2653 . A 0 . DP=23;I16=15,6,0,0,759,28489,0,0,1198,70082,0,0,389,8395,0,0;QS=3,0;MQSB=0.940481;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,73:2:0 0,9,109:3:0 17 2654 . C 0 . DP=23;I16=16,7,0,0,836,31006,0,0,1318,77282,0,0,393,8385,0,0;QS=3,0;MQSB=0.955805;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,9,88:3:0 0,9,99:3:0 17 2655 . C 0 . DP=23;I16=16,7,0,0,774,27190,0,0,1318,77282,0,0,392,8364,0,0;QS=3,0;MQSB=0.955805;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,9,83:3:0 0,9,95:3:0 17 2656 . G 0 . DP=24;I16=17,7,0,0,781,25689,0,0,1378,80882,0,0,390,8320,0,0;QS=3,0;MQSB=0.95083;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,116:4:0 0,9,74:3:0 17 2657 . C 0 . DP=24;I16=17,6,0,0,871,33435,0,0,1349,80041,0,0,381,8241,0,0;QS=3,0;MQSB=0.978183;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,9,98:3:0 0,9,99:3:0 17 2658 . T 0 . DP=23;I16=17,6,0,0,897,35255,0,0,1318,77282,0,0,389,8319,0,0;QS=3,0;MQSB=0.92854;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,133:4:0 0,9,107:3:0 17 2659 . A 0 . DP=23;I16=17,6,0,0,817,29729,0,0,1318,77282,0,0,389,8361,0,0;QS=3,0;MQSB=0.92854;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,120:4:0 0,9,93:3:0 17 2660 . G 0 . DP=23;I16=17,6,0,0,900,35462,0,0,1318,77282,0,0,389,8379,0,0;QS=3,0;MQSB=0.92854;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,126:4:0 0,9,94:3:0 17 2661 . A 0 . DP=24;I16=18,6,0,0,871,32185,0,0,1378,80882,0,0,390,8422,0,0;QS=3,0;MQSB=0.923116;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,127:4:0 0,9,104:3:0 17 2662 . T 0 . DP=24;I16=17,6,0,0,836,30812,0,0,1349,80041,0,0,366,7816,0,0;QS=3,0;MQSB=0.83771;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,122:4:0 0,9,99:3:0 17 2663 . A 0 . DP=24;I16=17,6,0,0,827,30583,0,0,1318,77282,0,0,389,8341,0,0;QS=3,0;MQSB=0.92854;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,104:4:0 0,9,101:3:0 17 2664 . C 0 . DP=23;I16=17,6,0,0,787,28083,0,0,1318,77282,0,0,388,8270,0,0;QS=3,0;MQSB=0.92854;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,107:4:0 0,9,91:3:0 17 2665 . G 0 . DP=23;I16=17,6,0,0,728,23290,0,0,1318,77282,0,0,386,8178,0,0;QS=3,0;MQSB=0.92854;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,112:4:0 0,9,78:3:0 17 2666 . T 0 . DP=23;I16=17,5,0,0,799,29273,0,0,1289,76441,0,0,367,7825,0,0;QS=3,0;MQSB=0.981001;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,105:3:0 0,9,94:3:0 17 2667 . C 0 . DP=23;I16=16,5,0,0,792,30190,0,0,1260,75600,0,0,345,7393,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,96:3:0 0,9,98:3:0 17 2668 . C 0 . DP=24;I16=18,6,0,0,832,29692,0,0,1378,80882,0,0,381,8069,0,0;QS=3,0;MQSB=0.923116;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,107:4:0 0,9,95:3:0 17 2669 . C 0 . DP=23;I16=18,5,0,0,829,30953,0,0,1318,77282,0,0,383,8087,0,0;QS=3,0;MQSB=0.889264;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,120:4:0 0,9,88:3:0 17 2670 . T 0 . DP=23;I16=17,5,0,0,841,32669,0,0,1289,76441,0,0,368,7828,0,0;QS=3,0;MQSB=0.801124;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,131:4:0 0,9,100:3:0 17 2671 . C 0 . DP=22;I16=17,5,0,0,842,32448,0,0,1258,73682,0,0,386,8110,0,0;QS=3,0;MQSB=0.895399;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,128:4:0 0,6,70:2:0 17 2672 . C 0 . DP=22;I16=17,5,0,0,808,30180,0,0,1258,73682,0,0,388,8164,0,0;QS=3,0;MQSB=0.895399;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,129:4:0 0,6,75:2:0 17 2673 . C 0 . DP=22;I16=17,5,0,0,842,32528,0,0,1258,73682,0,0,390,8246,0,0;QS=3,0;MQSB=0.895399;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,133:4:0 0,6,75:2:0 17 2674 . T 0 . DP=23;I16=17,6,0,0,860,33242,0,0,1318,77282,0,0,392,8356,0,0;QS=3,0;MQSB=0.92854;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,142:4:0 0,6,79:2:0 17 2675 . T 0 . DP=22;I16=16,6,0,0,756,26986,0,0,1258,73682,0,0,394,8392,0,0;QS=3,0;MQSB=0.934321;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,134:4:0 0,6,74:2:0 17 2676 . T 0 . DP=22;I16=16,6,0,0,774,28022,0,0,1258,73682,0,0,396,8452,0,0;QS=3,0;MQSB=0.934321;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,135:4:0 0,6,75:2:0 17 2677 . C 0 . DP=22;I16=16,6,0,0,866,34444,0,0,1258,73682,0,0,398,8536,0,0;QS=3,0;MQSB=0.934321;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,131:4:0 0,6,77:2:0 17 2678 . T 0 . DP=22;I16=16,5,0,0,818,32216,0,0,1229,72841,0,0,374,7970,0,0;QS=3,0;MQSB=0.978919;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,111:3:0 0,6,80:2:0 17 2679 . T 0 . DP=22;I16=16,6,0,0,808,29912,0,0,1258,73682,0,0,400,8680,0,0;QS=3,0;MQSB=0.934321;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,137:4:0 0,6,75:2:0 17 2680 . C 0 . DP=23;I16=16,7,0,0,873,33605,0,0,1318,77282,0,0,400,8740,0,0;QS=3,0;MQSB=0.955805;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,131:4:0 0,6,70:2:0 17 2681 . T 0 . DP=22;I16=15,7,0,0,820,31248,0,0,1258,73682,0,0,402,8824,0,0;QS=3,0;MQSB=0.961028;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,133:4:0 0,6,75:2:0 17 2682 . G 0 . DP=22;I16=15,7,0,0,831,31865,0,0,1258,73682,0,0,403,8881,0,0;QS=3,0;MQSB=0.961028;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,135:4:0 0,6,64:2:0 17 2683 . G 0 . DP=22;I16=13,6,0,0,702,26368,0,0,1109,65641,0,0,394,8926,0,0;QS=3,0;MQSB=0.850016;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,136:4:0 0,6,53:2:0 17 2684 . G 0 . DP=22;I16=14,7,0,0,754,27678,0,0,1229,72841,0,0,403,9057,0,0;QS=3,0;MQSB=0.872525;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,128:4:0 0,6,62:2:0 17 2685 . G 0 . DP=22;I16=15,7,0,0,736,25916,0,0,1258,73682,0,0,406,9184,0,0;QS=3,0;MQSB=0.961028;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,130:4:0 0,6,45:2:0 17 2686 . C 0 . DP=22;I16=15,7,0,0,803,29933,0,0,1258,73682,0,0,406,9278,0,0;QS=3,0;MQSB=0.961028;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,134:4:0 0,6,68:2:0 17 2687 . A 0 . DP=21;I16=13,7,0,0,716,26356,0,0,1169,69241,0,0,406,9340,0,0;QS=3,0;MQSB=0.875769;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,136:4:0 0,6,61:2:0 17 2688 . C 0 . DP=20;I16=13,7,0,0,750,28308,0,0,1169,69241,0,0,407,9417,0,0;QS=3,0;MQSB=0.875769;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,134:4:0 0,6,66:2:0 17 2689 . A 0 . DP=19;I16=12,7,0,0,741,29105,0,0,1109,65641,0,0,409,9507,0,0;QS=3,0;MQSB=0.879351;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,140:4:0 0,6,73:2:0 17 2690 . G 0 . DP=20;I16=12,8,0,0,751,28929,0,0,1169,69241,0,0,411,9609,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,135:4:0 0,6,61:2:0 17 2691 . G 0 . DP=20;I16=12,7,0,0,682,25006,0,0,1109,65641,0,0,403,9603,0,0;QS=3,0;MQSB=0.879351;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,132:4:0 0,6,63:2:0 17 2692 . T 0 . DP=20;I16=12,8,0,0,648,21758,0,0,1169,69241,0,0,417,9853,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,125:4:0 0,6,72:2:0 17 2693 . C 0 . DP=20;I16=12,8,0,0,779,30645,0,0,1169,69241,0,0,418,9898,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,130:4:0 0,6,70:2:0 17 2694 . A 0 . DP=21;I16=13,8,0,0,723,25649,0,0,1229,72841,0,0,417,9859,0,0;QS=3,0;MQSB=0.895122;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,126:4:0 0,6,73:2:0 17 2695 . C 0 . DP=20;I16=12,8,0,0,757,28835,0,0,1169,69241,0,0,418,9834,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,130:4:0 0,6,63:2:0 17 2696 . A 0 . DP=20;I16=12,8,0,0,733,27357,0,0,1169,69241,0,0,419,9823,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,130:4:0 0,6,69:2:0 17 2697 . C 0 . DP=20;I16=12,8,0,0,738,27604,0,0,1169,69241,0,0,419,9777,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,128:4:0 0,6,66:2:0 17 2698 . T 0 . DP=21;I16=12,8,0,0,788,31268,0,0,1169,69241,0,0,419,9747,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,144:4:0 0,6,77:2:0 17 2699 . C 0 . DP=22;I16=13,9,0,0,864,34148,0,0,1258,73682,0,0,443,10309,0,0;QS=3,0;MQSB=0.686045;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,135:4:0 0,9,98:3:0 17 2700 . T 0 . DP=22;I16=13,9,0,0,850,33296,0,0,1258,73682,0,0,444,10310,0,0;QS=3,0;MQSB=0.686045;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,141:4:0 0,9,105:3:0 17 2701 . C 0 . DP=22;I16=13,9,0,0,863,34157,0,0,1258,73682,0,0,444,10278,0,0;QS=3,0;MQSB=0.686045;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,140:4:0 0,9,94:3:0 17 2702 . T 0 . DP=22;I16=13,9,0,0,837,32525,0,0,1258,73682,0,0,444,10262,0,0;QS=3,0;MQSB=0.686045;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,143:4:0 0,9,83:3:0 17 2703 . T 0 . DP=21;I16=12,8,0,0,717,25881,0,0,1169,69241,0,0,420,9636,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,130:4:0 0,3,32:1:0 17 2704 . C 0 . DP=21;I16=12,9,0,0,794,30766,0,0,1198,70082,0,0,445,10225,0,0;QS=3,0;MQSB=0.695144;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,137:4:0 0,6,55:2:0 17 2705 . C 0 . DP=21;I16=12,9,0,0,809,31649,0,0,1198,70082,0,0,445,10205,0,0;QS=3,0;MQSB=0.695144;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,136:4:0 0,6,56:2:0 17 2706 . A 0 . DP=22;I16=12,10,0,0,820,31386,0,0,1258,73682,0,0,444,10150,0,0;QS=3,0;MQSB=0.731218;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,141:4:0 0,6,70:2:0 17 2707 . G 0 . DP=22;I16=12,10,0,0,832,32104,0,0,1258,73682,0,0,444,10110,0,0;QS=3,0;MQSB=0.731218;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,139:4:0 0,6,60:2:0 17 2708 . G 0 . DP=22;I16=12,10,0,0,777,28329,0,0,1258,73682,0,0,444,10086,0,0;QS=3,0;MQSB=0.731218;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,136:4:0 0,6,57:2:0 17 2709 . T 0 . DP=22;I16=12,9,0,0,761,28341,0,0,1229,72841,0,0,418,9404,0,0;QS=3,0;MQSB=0.913101;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,137:4:0 0,3,39:1:0 17 2710 . C 0 . DP=23;I16=12,10,0,0,840,32478,0,0,1289,76441,0,0,417,9365,0,0;QS=3,0;MQSB=0.924723;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,134:4:0 0,3,38:1:0 17 2711 . T 0 . DP=24;I16=13,11,0,0,893,33721,0,0,1378,80882,0,0,442,9970,0,0;QS=3,0;MQSB=0.75304;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,12,138:4:0 0,6,72:2:0 17 2712 . A 0 . DP=24;I16=13,11,0,0,889,33333,0,0,1378,80882,0,0,443,9971,0,0;QS=3,0;MQSB=0.75304;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,12,141:4:0 0,6,65:2:0 17 2713 . G T, 0 . DP=25;I16=13,11,0,1,915,35485,14,196,1378,80882,29,841,419,9369,25,625;QS=2.72549,0.27451,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.569783;BQB=1;MQ0F=0 PL:DP:DV 0,57,255,57,255,255:19:0 0,12,131,12,131,131:4:0 8,0,31,11,34,42:2:1 17 2714 . G 0 . DP=25;I16=13,11,0,0,891,33457,0,0,1378,80882,0,0,444,9990,0,0;QS=3,0;MQSB=0.75304;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,12,132:4:0 0,6,64:2:0 17 2715 . A 0 . DP=25;I16=13,12,0,0,888,32012,0,0,1407,81723,0,0,446,10014,0,0;QS=3,0;MQSB=0.569783;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,12,135:4:0 0,6,63:2:0 17 2716 . T 0 . DP=26;I16=13,13,0,0,937,34241,0,0,1467,85323,0,0,446,10012,0,0;QS=3,0;MQSB=0.606531;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,12,134:4:0 0,9,90:3:0 17 2717 . G 0 . DP=26;I16=13,12,0,0,939,35995,0,0,1438,84482,0,0,422,9410,0,0;QS=3,0;MQSB=0.778801;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,12,134:4:0 0,6,72:2:0 17 2718 . C 0 . DP=24;I16=12,11,0,0,864,33118,0,0,1318,77282,0,0,425,9457,0,0;QS=3,0;MQSB=0.7613;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,130:4:0 0,6,65:2:0 17 2719 . A 0 . DP=24;I16=12,12,0,0,911,35371,0,0,1347,78123,0,0,452,10102,0,0;QS=3,0;MQSB=0.582748;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,139:4:0 0,9,99:3:0 17 2720 . G 0 . DP=24;I16=12,12,0,0,940,37044,0,0,1347,78123,0,0,453,10095,0,0;QS=3,0;MQSB=0.582748;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,136:4:0 0,9,97:3:0 17 2721 . C 0 . DP=24;I16=12,11,0,0,881,34499,0,0,1318,77282,0,0,429,9485,0,0;QS=3,0;MQSB=0.7613;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,131:4:0 0,6,76:2:0 17 2722 . T 0 . DP=24;I16=11,13,0,0,898,34310,0,0,1347,78123,0,0,456,10146,0,0;QS=3,0;MQSB=0.633229;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,156:5:0 0,9,98:3:0 17 2723 . G 0 . DP=25;I16=12,13,0,0,941,36257,0,0,1407,81723,0,0,459,10203,0,0;QS=3,0;MQSB=0.619223;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,184:6:0 0,9,76:3:0 17 2724 . A 0 . DP=25;I16=12,13,0,0,917,34211,0,0,1407,81723,0,0,462,10234,0,0;QS=3,0;MQSB=0.619223;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,188:6:0 0,9,84:3:0 17 2725 . G 0 . DP=25;I16=12,12,0,0,916,35656,0,0,1378,80882,0,0,438,9566,0,0;QS=3,0;MQSB=0.786628;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,185:6:0 0,6,67:2:0 17 2726 . G 0 . DP=25;I16=11,12,0,0,841,31809,0,0,1287,74523,0,0,437,9545,0,0;QS=3,0;MQSB=0.597127;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,186:6:0 0,6,46:2:0 17 2727 . G 0 . DP=25;I16=12,13,0,0,889,32233,0,0,1407,81723,0,0,464,10182,0,0;QS=3,0;MQSB=0.619223;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,194:7:0 0,6,56:2:0 17 2728 . G 0 . DP=25;I16=12,13,0,0,883,32287,0,0,1407,81723,0,0,467,10219,0,0;QS=3,0;MQSB=0.619223;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,189:7:0 0,6,50:2:0 17 2729 . T 0 . DP=25;I16=12,13,0,0,871,31019,0,0,1407,81723,0,0,469,10233,0,0;QS=3,0;MQSB=0.619223;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,189:7:0 0,6,62:2:0 17 2730 . G 0 . DP=25;I16=12,13,0,0,907,34299,0,0,1407,81723,0,0,471,10275,0,0;QS=3,0;MQSB=0.619223;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,192:7:0 0,6,49:2:0 17 2731 . C 0 . DP=25;I16=12,13,0,0,893,33027,0,0,1407,81723,0,0,473,10345,0,0;QS=3,0;MQSB=0.619223;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,192:7:0 0,6,52:2:0 17 2732 . C 0 . DP=25;I16=12,13,0,0,882,32170,0,0,1407,81723,0,0,473,10343,0,0;QS=3,0;MQSB=0.619223;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,195:7:0 0,6,38:2:0 17 2733 . C A, 0 . DP=25;I16=12,12,0,1,835,30063,13,169,1378,80882,29,841,448,9744,25,625;QS=2.64865,0.351351,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.619223;BQB=1;MQ0F=0 PL:DP:DV 0,48,255,48,255,255:16:0 0,21,187,21,187,187:7:0 7,0,18,10,21,28:2:1 17 2734 . C 0 . DP=25;I16=12,12,0,0,890,33726,0,0,1378,80882,0,0,449,9797,0,0;QS=3,0;MQSB=0.786628;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,24,208:8:0 0,3,39:1:0 17 2735 . T 0 . DP=26;I16=13,12,0,0,955,36875,0,0,1438,84482,0,0,451,9877,0,0;QS=3,0;MQSB=0.778801;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,24,228:8:0 0,3,41:1:0 17 2736 . C 0 . DP=26;I16=13,11,0,0,921,35553,0,0,1409,83641,0,0,428,9308,0,0;QS=3,0;MQSB=0.931547;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,24,220:8:0 0,3,33:1:0 17 2737 . T 0 . DP=26;I16=13,13,0,0,967,36581,0,0,1467,85323,0,0,475,10403,0,0;QS=3,0;MQSB=0.606531;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,24,228:8:0 0,6,64:2:0 17 2738 . T 0 . DP=26;I16=13,13,0,0,895,31873,0,0,1467,85323,0,0,474,10374,0,0;QS=3,0;MQSB=0.606531;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,24,201:8:0 0,6,47:2:0 17 2739 . A 0 . DP=26;I16=13,12,0,0,871,31051,0,0,1407,81723,0,0,448,9698,0,0;QS=3,0;MQSB=0.569783;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,224:8:0 0,9,78:3:0 17 2740 . C 0 . DP=26;I16=14,11,0,0,952,36456,0,0,1438,84482,0,0,448,9674,0,0;QS=3,0;MQSB=0.745495;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,24,216:8:0 0,6,71:2:0 17 2741 . C 0 . DP=26;I16=14,11,0,0,945,36141,0,0,1438,84482,0,0,448,9678,0,0;QS=3,0;MQSB=0.745495;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,24,215:8:0 0,6,75:2:0 17 2742 . A 0 . DP=27;I16=15,12,0,0,949,34263,0,0,1527,88923,0,0,472,10284,0,0;QS=3,0;MQSB=0.547344;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,27,230:9:0 0,9,92:3:0 17 2743 . T 0 . DP=27;I16=15,12,0,0,955,34479,0,0,1527,88923,0,0,471,10243,0,0;QS=3,0;MQSB=0.547344;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,27,224:9:0 0,9,99:3:0 17 2744 . C 0 . DP=26;I16=15,10,0,0,955,36951,0,0,1438,84482,0,0,445,9557,0,0;QS=3,0;MQSB=0.707404;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,234:9:0 0,6,76:2:0 17 2745 . T 0 . DP=26;I16=15,11,0,0,962,36786,0,0,1467,85323,0,0,469,10151,0,0;QS=3,0;MQSB=0.505697;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,243:9:0 0,9,104:3:0 17 2746 . A 0 . DP=26;I16=15,10,0,0,912,33536,0,0,1438,84482,0,0,443,9525,0,0;QS=3,0;MQSB=0.707404;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,235:9:0 0,6,78:2:0 17 2747 . A 0 . DP=26;I16=15,11,0,0,945,35117,0,0,1467,85323,0,0,467,10179,0,0;QS=3,0;MQSB=0.505697;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,238:9:0 0,9,96:3:0 17 2748 . T 0 . DP=27;I16=15,12,0,0,1005,37901,0,0,1527,88923,0,0,465,10187,0,0;QS=3,0;MQSB=0.547344;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,27,246:9:0 0,9,103:3:0 17 2749 . C 0 . DP=26;I16=14,12,0,0,975,37353,0,0,1467,85323,0,0,463,10123,0,0;QS=3,0;MQSB=0.558035;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,232:9:0 0,9,98:3:0 17 2750 . T 0 . DP=26;I16=15,11,0,0,982,37714,0,0,1498,88082,0,0,462,10086,0,0;QS=3,0;MQSB=0.738577;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,227:8:0 0,12,130:4:0 17 2751 . G 0 . DP=26;I16=15,10,0,0,945,36031,0,0,1469,87241,0,0,437,9451,0,0;QS=3,0;MQSB=0.9171;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,205:8:0 0,9,103:3:0 17 2752 . T 0 . DP=26;I16=15,11,0,0,870,30288,0,0,1498,88082,0,0,460,9998,0,0;QS=3,0;MQSB=0.738577;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,189:8:0 0,12,119:4:0 17 2753 . G 0 . DP=26;I16=15,11,0,0,867,30913,0,0,1498,88082,0,0,458,9948,0,0;QS=3,0;MQSB=0.738577;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,202:8:0 0,12,116:4:0 17 2754 . C 0 . DP=25;I16=14,10,0,0,873,32607,0,0,1409,83641,0,0,436,9484,0,0;QS=3,0;MQSB=0.919431;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,24,205:8:0 0,9,104:3:0 17 2755 . C 0 . DP=25;I16=14,10,0,0,865,32243,0,0,1409,83641,0,0,436,9528,0,0;QS=3,0;MQSB=0.919431;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,24,209:8:0 0,9,97:3:0 17 2756 . C 0 . DP=25;I16=14,9,0,0,838,31276,0,0,1380,82800,0,0,411,8971,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,24,204:8:0 0,9,97:3:0 17 2757 . T 0 . DP=25;I16=14,11,0,0,904,33980,0,0,1438,84482,0,0,455,10011,0,0;QS=3,0;MQSB=0.745495;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,212:7:0 0,15,147:5:0 17 2758 . T 0 . DP=25;I16=14,10,0,0,841,30293,0,0,1409,83641,0,0,439,9801,0,0;QS=3,0;MQSB=0.919431;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,195:7:0 0,12,133:4:0 17 2759 . A 0 . DP=25;I16=14,11,0,0,884,31728,0,0,1438,84482,0,0,457,10195,0,0;QS=3,0;MQSB=0.745495;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,192:7:0 0,15,147:5:0 17 2760 . T 0 . DP=25;I16=14,11,0,0,907,33965,0,0,1438,84482,0,0,457,10275,0,0;QS=3,0;MQSB=0.745495;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,204:7:0 0,15,150:5:0 17 2761 . T 0 . DP=23;I16=13,9,0,0,838,32530,0,0,1289,76441,0,0,444,10130,0,0;QS=3,0;MQSB=0.910098;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,185:6:0 0,12,141:4:0 17 2762 . T 0 . DP=24;I16=13,11,0,0,869,32261,0,0,1378,80882,0,0,459,10395,0,0;QS=3,0;MQSB=0.75304;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,178:6:0 0,18,177:6:0 17 2763 . C A, 0 . DP=24;I16=13,10,0,1,843,31711,13,169,1349,80041,29,841,448,10290,12,144;QS=2.93333,0.0666667,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.75304;BQB=1;MQ0F=0 PL:DP:DV 0,36,255,36,255,255:12:0 0,18,170,18,170,170:6:0 0,5,147,15,150,153:6:1 17 2764 . C 0 . DP=24;I16=13,10,0,0,854,32376,0,0,1349,80041,0,0,450,10374,0,0;QS=3,0;MQSB=0.921963;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,163:6:0 0,15,162:5:0 17 2765 . T 0 . DP=24;I16=12,11,0,0,853,32173,0,0,1318,77282,0,0,457,10469,0,0;QS=3,0;MQSB=0.7613;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,154:5:0 0,18,189:6:0 17 2766 . C 0 . DP=24;I16=12,11,0,0,887,34485,0,0,1349,80041,0,0,448,10398,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,160:5:0 0,18,186:6:0 17 2767 . T 0 . DP=24;I16=12,12,0,0,905,34757,0,0,1378,80882,0,0,458,10510,0,0;QS=3,0;MQSB=0.786628;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,153:5:0 0,21,217:7:0 17 2768 . G 0 . DP=23;I16=11,11,0,0,852,33258,0,0,1289,76441,0,0,452,10462,0,0;QS=3,0;MQSB=0.936864;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,141:5:0 0,18,197:6:0 17 2769 . C 0 . DP=23;I16=11,12,0,0,832,31390,0,0,1318,77282,0,0,459,10481,0,0;QS=3,0;MQSB=0.795196;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,142:5:0 0,21,202:7:0 17 2770 . T 0 . DP=23;I16=11,12,0,0,828,30854,0,0,1318,77282,0,0,459,10471,0,0;QS=3,0;MQSB=0.795196;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,152:5:0 0,21,199:7:0 17 2771 . T 0 . DP=23;I16=11,11,0,0,817,30957,0,0,1258,73682,0,0,454,10456,0,0;QS=3,0;MQSB=0.770381;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,154:5:0 0,18,198:6:0 17 2772 . T 0 . DP=23;I16=11,12,0,0,856,32206,0,0,1318,77282,0,0,459,10511,0,0;QS=3,0;MQSB=0.795196;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,160:5:0 0,21,210:7:0 17 2773 . A 0 . DP=23;I16=11,12,0,0,828,30664,0,0,1318,77282,0,0,459,10561,0,0;QS=3,0;MQSB=0.795196;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,140:5:0 0,21,209:7:0 17 2774 . G 0 . DP=22;I16=11,11,0,0,843,32715,0,0,1258,73682,0,0,458,10530,0,0;QS=3,0;MQSB=0.770381;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,154:5:0 0,21,207:7:0 17 2775 . T 0 . DP=22;I16=10,11,0,0,748,27720,0,0,1198,70082,0,0,432,9892,0,0;QS=3,0;MQSB=0.780412;MQ0F=0 PL:DP:DV 0,30,253:10:0 0,12,115:4:0 0,21,203:7:0 17 2776 . G 0 . DP=22;I16=12,10,0,0,803,30251,0,0,1289,76441,0,0,456,10470,0,0;QS=3,0;MQSB=0.924723;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,135:5:0 0,21,210:7:0 17 2777 . A 0 . DP=22;I16=12,10,0,0,852,33524,0,0,1289,76441,0,0,456,10438,0,0;QS=3,0;MQSB=0.924723;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,148:5:0 0,21,220:7:0 17 2778 . G 0 . DP=23;I16=12,11,0,0,861,33049,0,0,1349,80041,0,0,456,10422,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,154:5:0 0,21,224:7:0 17 2779 . G 0 . DP=23;I16=11,11,0,0,834,32014,0,0,1289,76441,0,0,432,9798,0,0;QS=3,0;MQSB=0.936864;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,136:4:0 0,21,219:7:0 17 2780 . A 0 . DP=23;I16=11,11,0,0,792,29162,0,0,1289,76441,0,0,433,9817,0,0;QS=3,0;MQSB=0.936864;MQ0F=0 PL:DP:DV 0,33,244:11:0 0,12,133:4:0 0,21,215:7:0 17 2781 . A 0 . DP=23;I16=11,11,0,0,844,33092,0,0,1289,76441,0,0,441,10141,0,0;QS=3,0;MQSB=0.936864;MQ0F=0 PL:DP:DV 0,30,253:10:0 0,15,151:5:0 0,21,233:7:0 17 2782 . G 0 . DP=23;I16=12,11,0,0,840,31826,0,0,1349,80041,0,0,458,10438,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,154:5:0 0,21,218:7:0 17 2783 . A 0 . DP=23;I16=11,11,0,0,860,33888,0,0,1289,76441,0,0,432,9790,0,0;QS=3,0;MQSB=0.936864;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,142:4:0 0,21,230:7:0 17 2784 . G 0 . DP=23;I16=12,11,0,0,860,33092,0,0,1349,80041,0,0,456,10410,0,0;QS=3,0;MQSB=0.934091;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,143:5:0 0,21,212:7:0 17 2785 . G 0 . DP=23;I16=11,11,0,0,797,29747,0,0,1289,76441,0,0,429,9749,0,0;QS=3,0;MQSB=0.936864;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,134:4:0 0,21,204:7:0 17 2786 . C 0 . DP=24;I16=12,12,0,0,831,29497,0,0,1409,83641,0,0,451,10309,0,0;QS=3,0;MQSB=0.941765;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,141:5:0 0,21,199:7:0 17 2787 . C 0 . DP=24;I16=11,12,0,0,792,28454,0,0,1349,80041,0,0,424,9642,0,0;QS=3,0;MQSB=0.944319;MQ0F=0 PL:DP:DV 0,36,252:12:0 0,12,127:4:0 0,21,200:7:0 17 2788 . C 0 . DP=23;I16=11,11,0,0,791,29517,0,0,1289,76441,0,0,421,9523,0,0;QS=3,0;MQSB=0.936864;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,125:4:0 0,21,209:7:0 17 2789 . C 0 . DP=24;I16=13,11,0,0,880,33470,0,0,1409,83641,0,0,443,10051,0,0;QS=3,0;MQSB=0.931547;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,150:5:0 0,21,226:7:0 17 2790 . T 0 . DP=23;I16=13,10,0,0,886,34738,0,0,1349,80041,0,0,442,9976,0,0;QS=3,0;MQSB=0.921963;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,165:5:0 0,21,227:7:0 17 2791 . G 0 . DP=23;I16=13,10,0,0,873,34037,0,0,1349,80041,0,0,441,9923,0,0;QS=3,0;MQSB=0.921963;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,155:5:0 0,21,226:7:0 17 2792 . G 0 . DP=23;I16=13,10,0,0,832,30870,0,0,1349,80041,0,0,438,9792,0,0;QS=3,0;MQSB=0.921963;MQ0F=0 PL:DP:DV 0,33,254:11:0 0,15,146:5:0 0,21,221:7:0 17 2793 . T 0 . DP=23;I16=13,10,0,0,827,30693,0,0,1349,80041,0,0,435,9683,0,0;QS=3,0;MQSB=0.921963;MQ0F=0 PL:DP:DV 0,33,247:11:0 0,15,157:5:0 0,21,214:7:0 17 2794 . C 0 . DP=22;I16=12,10,0,0,786,29122,0,0,1289,76441,0,0,433,9595,0,0;QS=3,0;MQSB=0.924723;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,145:5:0 0,21,201:7:0 17 2795 . C 0 . DP=22;I16=11,10,0,0,814,31804,0,0,1229,72841,0,0,428,9518,0,0;QS=3,0;MQSB=0.927743;MQ0F=0 PL:DP:DV 0,27,240:9:0 0,15,159:5:0 0,21,222:7:0 17 2796 . A 0 . DP=22;I16=11,10,0,0,762,28412,0,0,1229,72841,0,0,427,9475,0,0;QS=3,0;MQSB=0.927743;MQ0F=0 PL:DP:DV 0,27,221:9:0 0,15,156:5:0 0,21,211:7:0 17 2797 . T 0 . DP=22;I16=12,10,0,0,792,29116,0,0,1289,76441,0,0,427,9451,0,0;QS=3,0;MQSB=0.924723;MQ0F=0 PL:DP:DV 0,30,243:10:0 0,15,148:5:0 0,21,214:7:0 17 2798 . G 0 . DP=22;I16=12,10,0,0,817,31105,0,0,1289,76441,0,0,424,9394,0,0;QS=3,0;MQSB=0.924723;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,152:5:0 0,21,205:7:0 17 2799 . A 0 . DP=21;I16=11,10,0,0,788,30210,0,0,1229,72841,0,0,421,9309,0,0;QS=3,0;MQSB=0.927743;MQ0F=0 PL:DP:DV 0,27,220:9:0 0,15,167:5:0 0,21,218:7:0 17 2800 . A 0 . DP=21;I16=11,10,0,0,836,33616,0,0,1229,72841,0,0,418,9246,0,0;QS=3,0;MQSB=0.927743;MQ0F=0 PL:DP:DV 0,27,237:9:0 0,15,164:5:0 0,21,237:7:0 17 2801 . G 0 . DP=21;I16=11,10,0,0,792,30182,0,0,1229,72841,0,0,415,9205,0,0;QS=3,0;MQSB=0.927743;MQ0F=0 PL:DP:DV 0,27,234:9:0 0,15,157:5:0 0,21,214:7:0 17 2802 . G 0 . DP=21;I16=11,9,0,0,729,27275,0,0,1169,69241,0,0,387,8559,0,0;QS=3,0;MQSB=0.916401;MQ0F=0 PL:DP:DV 0,24,229:8:0 0,15,135:5:0 0,21,215:7:0 17 2803 . G 0 . DP=21;I16=11,10,0,0,737,26709,0,0,1229,72841,0,0,406,9036,0,0;QS=3,0;MQSB=0.927743;MQ0F=0 PL:DP:DV 0,27,233:9:0 0,15,125:5:0 0,21,207:7:0 17 2804 . G 0 . DP=21;I16=11,10,0,0,727,26103,0,0,1229,72841,0,0,400,8908,0,0;QS=3,0;MQSB=0.927743;MQ0F=0 PL:DP:DV 0,27,218:9:0 0,15,129:5:0 0,21,210:7:0 17 2805 . C 0 . DP=21;I16=10,10,0,0,714,26194,0,0,1169,69241,0,0,372,8316,0,0;QS=3,0;MQSB=0.931063;MQ0F=0 PL:DP:DV 0,27,229:9:0 0,12,111:4:0 0,21,205:7:0 17 2806 . C 0 . DP=20;I16=11,8,0,0,682,25074,0,0,1109,65641,0,0,379,8611,0,0;QS=3,0;MQSB=0.902014;MQ0F=0 PL:DP:DV 0,24,215:8:0 0,15,138:5:0 0,18,191:6:0 17 2807 . T 0 . DP=20;I16=11,9,0,0,755,29373,0,0,1169,69241,0,0,384,8640,0,0;QS=3,0;MQSB=0.916401;MQ0F=0 PL:DP:DV 0,24,221:8:0 0,15,152:5:0 0,21,227:7:0 17 2808 . T 0 . DP=20;I16=11,9,0,0,749,28383,0,0,1169,69241,0,0,379,8587,0,0;QS=3,0;MQSB=0.916401;MQ0F=0 PL:DP:DV 0,24,209:8:0 0,15,157:5:0 0,21,225:7:0 17 2809 . T 0 . DP=20;I16=11,8,0,0,697,26019,0,0,1109,65641,0,0,349,7927,0,0;QS=3,0;MQSB=0.902014;MQ0F=0 PL:DP:DV 0,21,189:7:0 0,15,148:5:0 0,21,226:7:0 17 2810 . C 0 . DP=20;I16=12,8,0,0,754,28900,0,0,1169,69241,0,0,368,8436,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,24,229:8:0 0,15,150:5:0 0,21,215:7:0 17 2811 . A 0 . DP=19;I16=11,8,0,0,759,30405,0,0,1109,65641,0,0,364,8340,0,0;QS=3,0;MQSB=0.902014;MQ0F=0 PL:DP:DV 0,21,209:7:0 0,15,166:5:0 0,21,228:7:0 17 2812 . G 0 . DP=20;I16=11,9,0,0,758,29062,0,0,1169,69241,0,0,359,8213,0,0;QS=3,0;MQSB=0.916401;MQ0F=0 PL:DP:DV 0,24,229:8:0 0,15,150:5:0 0,21,216:7:0 17 2813 . A 0 . DP=19;I16=11,8,0,0,702,27106,0,0,1140,68400,0,0,356,8104,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,195:7:0 0,15,160:5:0 0,21,217:7:0 17 2814 . G 0 . DP=19;I16=11,8,0,0,703,26579,0,0,1140,68400,0,0,353,8013,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,217:7:0 0,15,147:5:0 0,21,204:7:0 17 2815 . A 0 . DP=19;I16=10,7,0,0,597,21979,0,0,1020,61200,0,0,326,7470,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,170:6:0 0,12,122:4:0 0,21,208:7:0 17 2816 . C 0 . DP=19;I16=11,8,0,0,630,21880,0,0,1140,68400,0,0,343,7685,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,193:7:0 0,15,134:5:0 0,21,180:7:0 17 2817 . G 0 . DP=20;I16=11,8,0,0,612,20368,0,0,1140,68400,0,0,339,7547,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,200:7:0 0,15,115:5:0 0,21,178:7:0 17 2818 . G 0 . DP=20;I16=11,8,0,0,688,25280,0,0,1140,68400,0,0,335,7377,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,216:7:0 0,15,132:5:0 0,21,206:7:0 17 2819 . G 0 . DP=20;I16=11,8,0,0,696,25880,0,0,1140,68400,0,0,331,7227,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,222:7:0 0,15,142:5:0 0,21,195:7:0 17 2820 . G 0 . DP=20;I16=10,8,0,0,624,22228,0,0,1080,64800,0,0,320,7048,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,205:7:0 0,12,119:4:0 0,21,188:7:0 17 2821 . A 0 . DP=20;I16=9,7,0,0,496,16222,0,0,960,57600,0,0,279,6157,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,159:6:0 0,9,93:3:0 0,21,171:7:0 17 2822 . C 0 . DP=19;I16=8,9,0,0,543,18163,0,0,1020,61200,0,0,310,7064,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,203:7:0 0,9,79:3:0 0,21,168:7:0 17 2823 . C 0 . DP=18;I16=9,7,0,0,550,19506,0,0,960,57600,0,0,300,6640,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,201:7:0 0,6,58:2:0 0,21,192:7:0 17 2824 . C 0 . DP=18;I16=9,7,0,0,584,21560,0,0,960,57600,0,0,299,6567,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,219:8:0 0,3,37:1:0 0,21,210:7:0 17 2825 . C 0 . DP=18;I16=9,8,0,0,606,22286,0,0,1020,61200,0,0,324,7134,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,234:8:0 0,3,37:1:0 0,24,208:8:0 17 2826 . T 0 . DP=18;I16=9,8,0,0,604,21766,0,0,1020,61200,0,0,323,7043,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,222:8:0 0,3,33:1:0 0,24,220:8:0 17 2827 . G 0 . DP=18;I16=9,8,0,0,597,21643,0,0,1020,61200,0,0,322,6970,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,230:8:0 0,3,28:1:0 0,24,214:8:0 17 2828 . A 0 . DP=20;I16=9,9,0,0,598,20740,0,0,1080,64800,0,0,321,6915,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,213:9:0 0,3,36:1:0 0,24,219:8:0 17 2829 . G 0 . DP=20;I16=9,9,0,0,649,23719,0,0,1080,64800,0,0,305,6591,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,239:8:0 0,3,31:1:0 0,27,227:9:0 17 2830 . G 0 . DP=20;I16=9,10,0,0,661,23841,0,0,1140,68400,0,0,323,6867,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,248:9:0 0,3,34:1:0 0,27,222:9:0 17 2831 . A 0 . DP=21;I16=10,10,0,0,682,24052,0,0,1200,72000,0,0,324,6876,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,223:9:0 0,3,34:1:0 0,30,254:10:0 17 2832 . G 0 . DP=21;I16=10,8,0,0,626,22384,0,0,1080,64800,0,0,281,5883,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,230:8:0 0,3,31:1:0 0,27,223:9:0 17 2833 . C 0 . DP=21;I16=10,10,0,0,712,25708,0,0,1200,72000,0,0,327,6915,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,233:9:0 0,3,37:1:0 0,30,255:10:0 17 2834 . C 0 . DP=21;I16=10,9,0,0,674,24470,0,0,1140,68400,0,0,306,6464,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,230:8:0 0,3,36:1:0 0,30,243:10:0 17 2835 . C 0 . DP=20;I16=9,9,0,0,617,21835,0,0,1080,64800,0,0,305,6381,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,202:8:0 0,3,39:1:0 0,27,235:9:0 17 2836 . C 0 . DP=20;I16=9,9,0,0,580,19624,0,0,1080,64800,0,0,306,6412,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,206:8:0 0,3,39:1:0 0,27,200:9:0 17 2837 . G 0 . DP=21;I16=9,10,0,0,558,17614,0,0,1140,68400,0,0,330,7086,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,207:9:0 0,3,31:1:0 0,27,193:9:0 17 2838 . A 0 . DP=20;I16=9,10,0,0,640,22674,0,0,1140,68400,0,0,331,7065,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,245:9:0 0,3,17:1:0 0,27,220:9:0 17 2839 . G 0 . DP=20;I16=9,8,0,0,623,23267,0,0,1020,61200,0,0,282,5816,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,248:8:0 0,3,26:1:0 0,24,212:8:0 17 2840 . C 0 . DP=20;I16=9,10,0,0,644,22798,0,0,1140,68400,0,0,333,7089,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,233:9:0 0,3,32:1:0 0,27,227:9:0 17 2841 . A 0 . DP=20;I16=9,9,0,0,675,25801,0,0,1080,64800,0,0,309,6509,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,252:9:0 0,3,35:1:0 0,24,234:8:0 17 2842 . G 0 . DP=20;I16=9,10,0,0,688,25554,0,0,1140,68400,0,0,332,7056,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,3,31:1:0 0,27,233:9:0 17 2843 . C 0 . DP=20;I16=8,10,0,0,651,23969,0,0,1080,64800,0,0,309,6517,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,0,0:0:0 0,24,211:8:0 17 2844 . A 0 . DP=20;I16=8,11,0,0,685,25399,0,0,1140,68400,0,0,331,6969,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,0,0:0:0 0,27,231:9:0 17 2845 . G 0 . DP=20;I16=8,10,0,0,673,25399,0,0,1080,64800,0,0,311,6561,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,0,0:0:0 0,24,228:8:0 17 2846 . C 0 . DP=20;I16=8,10,0,0,617,22007,0,0,1080,64800,0,0,311,6577,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,243:10:0 0,0,0:0:0 0,24,218:8:0 17 2847 . C 0 . DP=20;I16=7,11,0,0,580,19468,0,0,1080,64800,0,0,321,6917,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,241:10:0 0,0,0:0:0 0,24,187:8:0 17 2848 . G 0 . DP=19;I16=7,10,0,0,542,17884,0,0,1020,61200,0,0,323,6999,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,218:9:0 0,0,0:0:0 0,24,191:8:0 17 2849 . T 0 . DP=19;I16=7,11,0,0,609,21717,0,0,1080,64800,0,0,341,7357,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,231:9:0 0,0,0:0:0 0,27,214:9:0 17 2850 . C 0 . DP=19;I16=7,11,0,0,574,18980,0,0,1080,64800,0,0,343,7461,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,234:9:0 0,0,0:0:0 0,27,188:9:0 17 2851 . G 0 . DP=17;I16=6,11,0,0,589,20831,0,0,1020,61200,0,0,346,7584,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,239:9:0 0,0,0:0:0 0,24,194:8:0 17 2852 . T 0 . DP=17;I16=6,11,0,0,625,23241,0,0,1020,61200,0,0,348,7676,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,244:9:0 0,0,0:0:0 0,24,214:8:0 17 2853 . G 0 . DP=17;I16=6,11,0,0,635,23949,0,0,1020,61200,0,0,349,7739,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,0,0:0:0 0,24,202:8:0 17 2854 . T 0 . DP=17;I16=6,11,0,0,602,21990,0,0,1020,61200,0,0,348,7722,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,238:9:0 0,0,0:0:0 0,24,207:8:0 17 2855 . C 0 . DP=17;I16=6,10,0,0,577,21539,0,0,960,57600,0,0,337,7623,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,0,0:0:0 0,21,185:7:0 17 2856 . T 0 . DP=17;I16=6,8,0,0,530,20570,0,0,840,50400,0,0,289,6507,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,252:9:0 0,0,0:0:0 0,15,167:5:0 17 2857 . C 0 . DP=18;I16=6,10,0,0,577,21641,0,0,960,57600,0,0,336,7664,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,0,0:0:0 0,21,185:7:0 17 2858 . A 0 . DP=19;I16=6,12,0,0,634,23006,0,0,1080,64800,0,0,355,8081,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,254:10:0 0,3,39:1:0 0,21,185:7:0 17 2859 . C 0 . DP=19;I16=6,13,0,0,646,23056,0,0,1140,68400,0,0,361,8139,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,3,26:1:0 0,24,194:8:0 17 2860 . C 0 . DP=19;I16=6,13,0,0,655,23687,0,0,1140,68400,0,0,360,8166,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,251:10:0 0,3,37:1:0 0,24,200:8:0 17 2861 . C 0 . DP=20;I16=7,12,0,0,708,26756,0,0,1140,68400,0,0,333,7537,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,3,38:1:0 0,21,203:7:0 17 2862 . A 0 . DP=20;I16=6,13,0,0,749,29753,0,0,1140,68400,0,0,332,7554,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,3,43:1:0 0,24,225:8:0 17 2863 . G 0 . DP=19;I16=7,12,0,0,707,26901,0,0,1140,68400,0,0,356,8166,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,3,35:1:0 0,21,195:7:0 17 2864 . G 0 . DP=19;I16=7,11,0,0,677,25833,0,0,1080,64800,0,0,352,8070,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,3,36:1:0 0,18,185:6:0 17 2865 . G 0 . DP=19;I16=7,12,0,0,671,24569,0,0,1140,68400,0,0,350,7994,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,3,27:1:0 0,21,193:7:0 17 2866 . T 0 . DP=18;I16=6,11,0,0,591,21071,0,0,1020,61200,0,0,338,7834,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,3,32:1:0 0,15,147:5:0 17 2867 . G 0 . DP=18;I16=7,11,0,0,655,24279,0,0,1080,64800,0,0,347,7889,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,6,58:2:0 0,15,150:5:0 17 2868 . T 0 . DP=18;I16=7,11,0,0,640,23702,0,0,1080,64800,0,0,347,7859,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,6,68:2:0 0,15,157:5:0 17 2869 . C 0 . DP=18;I16=7,11,0,0,655,24737,0,0,1080,64800,0,0,346,7794,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,6,56:2:0 0,15,165:5:0 17 2870 . T 0 . DP=18;I16=7,11,0,0,700,27480,0,0,1080,64800,0,0,345,7743,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,6,70:2:0 0,15,176:5:0 17 2871 . G 0 . DP=18;I16=7,11,0,0,700,27554,0,0,1080,64800,0,0,344,7706,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,6,68:2:0 0,15,172:5:0 17 2872 . A 0 . DP=18;I16=7,11,0,0,651,23869,0,0,1080,64800,0,0,343,7683,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,6,66:2:0 0,15,171:5:0 17 2873 . A 0 . DP=18;I16=7,11,0,0,664,25000,0,0,1080,64800,0,0,342,7674,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,6,61:2:0 0,15,159:5:0 17 2874 . A 0 . DP=18;I16=7,11,0,0,636,23286,0,0,1080,64800,0,0,341,7679,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,6,57:2:0 0,15,162:5:0 17 2875 . C 0 . DP=18;I16=7,11,0,0,664,25148,0,0,1080,64800,0,0,340,7698,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,6,63:2:0 0,15,166:5:0 17 2876 . A 0 . DP=18;I16=6,11,0,0,666,26684,0,0,1020,61200,0,0,314,7106,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,6,74:2:0 0,15,185:5:0 17 2877 . G 0 . DP=17;I16=6,11,0,0,659,26009,0,0,1020,61200,0,0,339,7777,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,6,67:2:0 0,12,148:4:0 17 2878 . A 0 . DP=17;I16=7,10,0,0,628,23656,0,0,1020,61200,0,0,340,7834,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,6,69:2:0 0,15,166:5:0 17 2879 . T 0 . DP=18;I16=7,11,0,0,671,25359,0,0,1080,64800,0,0,342,7902,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,253:10:0 0,9,96:3:0 0,15,175:5:0 17 2880 . G 0 . DP=19;I16=8,11,0,0,708,26694,0,0,1140,68400,0,0,345,7983,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,118:4:0 0,15,173:5:0 17 2881 . T 0 . DP=19;I16=6,11,0,0,618,23304,0,0,1020,61200,0,0,299,6829,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,209:8:0 0,12,124:4:0 0,15,178:5:0 17 2882 . G 0 . DP=19;I16=8,11,0,0,700,26464,0,0,1140,68400,0,0,353,8191,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,118:4:0 0,15,176:5:0 17 2883 . G 0 . DP=19;I16=8,11,0,0,707,26939,0,0,1140,68400,0,0,357,8319,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,121:4:0 0,15,175:5:0 17 2884 . A 0 . DP=19;I16=7,11,0,0,649,24531,0,0,1080,64800,0,0,335,7787,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,229:9:0 0,12,121:4:0 0,15,181:5:0 17 2885 . G 0 . DP=19;I16=8,11,0,0,737,29253,0,0,1140,68400,0,0,362,8470,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,113:4:0 0,15,182:5:0 17 2886 . G A, 0 . DP=18;I16=7,9,1,0,571,21281,20,400,960,57600,60,3600,334,7882,6,36;QS=2.76471,0.235294,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0 PL:DP:DV 0,27,236,27,236,236:9:0 11,0,51,17,54,66:3:1 0,15,171,15,171,171:5:0 17 2887 . T 0 . DP=19;I16=9,9,0,0,581,20407,0,0,1080,64800,0,0,341,7905,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,213:10:0 0,9,94:3:0 0,15,166:5:0 17 2888 . C 0 . DP=20;I16=10,10,0,0,714,26630,0,0,1200,72000,0,0,368,8532,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,94:4:0 0,15,159:5:0 17 2889 . T 0 . DP=19;I16=9,10,0,0,712,27106,0,0,1140,68400,0,0,372,8550,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,114:4:0 0,15,175:5:0 17 2890 . C 0 . DP=19;I16=9,10,0,0,613,20711,0,0,1140,68400,0,0,376,8584,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,247:10:0 0,12,95:4:0 0,15,139:5:0 17 2891 . G 0 . DP=20;I16=9,11,0,0,674,23754,0,0,1200,72000,0,0,380,8634,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,242:10:0 0,15,141:5:0 0,15,143:5:0 17 2892 . G 0 . DP=21;I16=9,12,0,0,731,26677,0,0,1260,75600,0,0,385,8701,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,142:5:0 0,15,164:5:0 17 2893 . G 0 . DP=21;I16=9,12,0,0,696,24498,0,0,1260,75600,0,0,389,8687,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,251:11:0 0,15,141:5:0 0,15,150:5:0 17 2894 . T 0 . DP=21;I16=9,12,0,0,716,25490,0,0,1260,75600,0,0,393,8693,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,137:5:0 0,15,155:5:0 17 2895 . G 0 . DP=22;I16=9,12,0,0,773,29225,0,0,1260,75600,0,0,372,8094,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,149:6:0 0,15,166:5:0 17 2896 . A 0 . DP=22;I16=8,12,0,0,732,27684,0,0,1200,72000,0,0,361,7885,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,151:5:0 0,15,163:5:0 17 2897 . G 0 . DP=22;I16=10,12,0,0,783,28783,0,0,1320,79200,0,0,407,8835,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,161:6:0 0,15,168:5:0 17 2898 . G 0 . DP=22;I16=10,12,0,0,724,25596,0,0,1320,79200,0,0,412,8926,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,254:11:0 0,18,143:6:0 0,15,169:5:0 17 2899 . C 0 . DP=22;I16=8,11,0,0,625,21143,0,0,1140,68400,0,0,356,7640,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,228:9:0 0,15,130:5:0 0,15,147:5:0 17 2900 . G 0 . DP=24;I16=11,13,0,0,782,26700,0,0,1440,86400,0,0,420,9078,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,164:6:0 0,15,153:5:0 17 2901 . T 0 . DP=24;I16=11,13,0,0,821,29195,0,0,1440,86400,0,0,426,9192,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,174:6:0 0,15,166:5:0 17 2902 . G 0 . DP=24;I16=11,13,0,0,898,34176,0,0,1440,86400,0,0,432,9334,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,171:6:0 0,15,164:5:0 17 2903 . G 0 . DP=24;I16=11,13,0,0,894,33764,0,0,1440,86400,0,0,437,9455,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,183:6:0 0,15,168:5:0 17 2904 . C 0 . DP=24;I16=11,13,0,0,892,34010,0,0,1440,86400,0,0,440,9506,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,176:6:0 0,15,169:5:0 17 2905 . T 0 . DP=25;I16=12,13,0,0,926,34994,0,0,1500,90000,0,0,442,9536,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,175:6:0 0,15,174:5:0 17 2906 . C 0 . DP=25;I16=12,13,0,0,960,37070,0,0,1500,90000,0,0,444,9544,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,178:6:0 0,15,173:5:0 17 2907 . A 0 . DP=25;I16=11,13,0,0,933,36707,0,0,1440,86400,0,0,429,9275,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,202:6:0 0,15,170:5:0 17 2908 . G 0 . DP=25;I16=12,13,0,0,943,36315,0,0,1500,90000,0,0,446,9548,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,167:6:0 0,15,164:5:0 17 2909 . A 0 . DP=25;I16=10,13,0,0,864,32764,0,0,1380,82800,0,0,425,9105,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,182:6:0 0,15,171:5:0 17 2910 . T 0 . DP=25;I16=11,13,0,0,870,32054,0,0,1440,86400,0,0,447,9575,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,181:6:0 0,15,166:5:0 17 2911 . A 0 . DP=25;I16=11,14,0,0,871,31227,0,0,1500,90000,0,0,473,10259,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,174:6:0 0,18,188:6:0 17 2912 . C 0 . DP=24;I16=11,13,0,0,899,34121,0,0,1440,86400,0,0,474,10298,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,173:6:0 0,18,189:6:0 17 2913 . A 0 . DP=24;I16=10,13,0,0,879,34357,0,0,1380,82800,0,0,449,9691,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,164:5:0 0,18,199:6:0 17 2914 . G 0 . DP=24;I16=11,13,0,0,899,34575,0,0,1440,86400,0,0,472,10262,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,170:6:0 0,18,187:6:0 17 2915 . G 0 . DP=24;I16=11,13,0,0,867,32523,0,0,1440,86400,0,0,470,10236,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,177:6:0 0,18,178:6:0 17 2916 . G 0 . DP=24;I16=11,12,0,0,813,29935,0,0,1380,82800,0,0,443,9613,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,173:6:0 0,15,155:5:0 17 2917 . A 0 . DP=25;I16=11,13,0,0,864,31926,0,0,1440,86400,0,0,459,10181,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,185:6:0 0,18,178:6:0 17 2918 . G 0 . DP=25;I16=13,12,0,0,903,33489,0,0,1500,90000,0,0,462,10122,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,165:6:0 0,18,170:6:0 17 2919 . T 0 . DP=25;I16=12,12,0,0,837,29647,0,0,1440,86400,0,0,443,9765,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,169:6:0 0,18,178:6:0 17 2920 . G 0 . DP=25;I16=12,12,0,0,850,31266,0,0,1440,86400,0,0,433,9389,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,132:5:0 0,18,180:6:0 17 2921 . G 0 . DP=25;I16=13,12,0,0,874,31518,0,0,1500,90000,0,0,455,9951,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,159:6:0 0,18,176:6:0 17 2922 . C 0 . DP=26;I16=13,12,0,0,872,31374,0,0,1500,90000,0,0,438,9718,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,160:6:0 0,18,172:6:0 17 2923 . C 0 . DP=26;I16=13,12,0,0,873,31687,0,0,1500,90000,0,0,437,9735,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,172:6:0 0,18,175:6:0 17 2924 . C 0 . DP=25;I16=13,12,0,0,940,36016,0,0,1500,90000,0,0,449,9921,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,173:6:0 0,18,184:6:0 17 2925 . A 0 . DP=25;I16=13,12,0,0,912,33580,0,0,1500,90000,0,0,448,9964,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,174:6:0 0,18,189:6:0 17 2926 . C 0 . DP=25;I16=13,12,0,0,902,33224,0,0,1500,90000,0,0,445,9931,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,176:6:0 0,18,174:6:0 17 2927 . A 0 . DP=25;I16=12,12,0,0,896,33828,0,0,1440,86400,0,0,417,9295,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,162:5:0 0,18,191:6:0 17 2928 . G 0 . DP=24;I16=13,11,0,0,862,32078,0,0,1440,86400,0,0,440,9930,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,170:6:0 0,18,161:6:0 17 2929 . C 0 . DP=23;I16=13,9,0,0,794,29716,0,0,1320,79200,0,0,430,9878,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,153:5:0 0,15,149:5:0 17 2930 . T 0 . DP=23;I16=13,10,0,0,822,30260,0,0,1380,82800,0,0,438,10006,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,168:6:0 0,15,157:5:0 17 2931 . C 0 . DP=23;I16=13,10,0,0,803,28963,0,0,1380,82800,0,0,436,10020,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,162:6:0 0,15,140:5:0 17 2932 . G 0 . DP=22;I16=12,10,0,0,728,25158,0,0,1320,79200,0,0,435,10049,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,171:6:0 0,12,117:4:0 17 2933 . G 0 . DP=22;I16=11,10,0,0,760,28336,0,0,1260,75600,0,0,430,10034,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,175:6:0 0,12,116:4:0 17 2934 . C 0 . DP=21;I16=12,9,0,0,759,28241,0,0,1260,75600,0,0,432,10052,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,161:6:0 0,9,104:3:0 17 2935 . C 0 . DP=21;I16=12,9,0,0,766,28494,0,0,1260,75600,0,0,431,10075,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,178:6:0 0,9,94:3:0 17 2936 . T 0 . DP=21;I16=11,9,0,0,760,29284,0,0,1200,72000,0,0,429,10063,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,188:6:0 0,9,98:3:0 17 2937 . G 0 . DP=20;I16=11,9,0,0,728,27374,0,0,1200,72000,0,0,428,10066,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,177:6:0 0,9,79:3:0 17 2938 . T 0 . DP=20;I16=11,9,0,0,719,26217,0,0,1200,72000,0,0,427,10083,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,188:6:0 0,9,101:3:0 17 2939 . C 0 . DP=19;I16=11,8,0,0,729,28277,0,0,1140,68400,0,0,427,10113,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,163:5:0 0,9,106:3:0 17 2940 . T 0 . DP=19;I16=11,8,0,0,739,29133,0,0,1140,68400,0,0,427,10155,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,171:5:0 0,9,109:3:0 17 2941 . T 0 . DP=19;I16=11,8,0,0,702,26358,0,0,1140,68400,0,0,427,10209,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,170:5:0 0,9,99:3:0 17 2942 . T 0 . DP=19;I16=11,8,0,0,695,25671,0,0,1140,68400,0,0,427,10275,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,169:5:0 0,9,100:3:0 17 2943 . G 0 . DP=18;I16=11,6,0,0,621,23187,0,0,1020,61200,0,0,401,9627,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,228:9:0 0,15,170:5:0 0,9,86:3:0 17 2944 . A 0 . DP=18;I16=10,7,0,0,642,24406,0,0,1020,61200,0,0,399,9563,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,239:9:0 0,15,168:5:0 0,9,111:3:0 17 2945 . A 0 . DP=18;I16=11,7,0,0,637,23199,0,0,1080,64800,0,0,422,10132,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,242:10:0 0,15,152:5:0 0,9,107:3:0 17 2946 . A 0 . DP=18;I16=11,7,0,0,692,26794,0,0,1080,64800,0,0,420,10084,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,174:5:0 0,9,108:3:0 17 2947 . G 0 . DP=18;I16=11,7,0,0,639,23329,0,0,1080,64800,0,0,418,10044,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,160:5:0 0,9,78:3:0 17 2948 . G 0 . DP=19;I16=11,8,0,0,702,26272,0,0,1140,68400,0,0,415,9961,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,163:5:0 0,12,116:4:0 17 2949 . C 0 . DP=19;I16=10,8,0,0,657,24565,0,0,1080,64800,0,0,388,9260,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,125:4:0 0,12,124:4:0 17 2950 . C 0 . DP=19;I16=11,8,0,0,667,24359,0,0,1140,68400,0,0,411,9817,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,131:5:0 0,12,115:4:0 17 2951 . A 0 . DP=20;I16=11,8,0,0,682,24956,0,0,1140,68400,0,0,409,9757,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,250:10:0 0,15,149:5:0 0,12,133:4:0 17 2952 . C 0 . DP=20;I16=11,9,0,0,647,21709,0,0,1200,72000,0,0,408,9706,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,128:5:0 0,12,112:4:0 17 2953 . G 0 . DP=21;I16=11,9,0,0,620,19954,0,0,1200,72000,0,0,407,9665,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,237:11:0 0,15,132:5:0 0,12,111:4:0 17 2954 . T 0 . DP=21;I16=11,10,0,0,742,26662,0,0,1260,75600,0,0,414,9666,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,175:6:0 0,12,135:4:0 17 2955 . G 0 . DP=22;I16=11,11,0,0,787,28475,0,0,1320,79200,0,0,412,9568,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,170:6:0 0,12,127:4:0 17 2956 . A 0 . DP=22;I16=11,11,0,0,747,26449,0,0,1320,79200,0,0,410,9438,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,161:6:0 0,12,125:4:0 17 2957 . C 0 . DP=22;I16=10,10,0,0,693,24757,0,0,1200,72000,0,0,358,8078,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,143:6:0 0,9,104:3:0 17 2958 . C 0 . DP=21;I16=11,10,0,0,784,29744,0,0,1260,75600,0,0,407,9237,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,172:6:0 0,12,135:4:0 17 2959 . T 0 . DP=21;I16=11,10,0,0,791,30411,0,0,1260,75600,0,0,406,9164,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,182:6:0 0,12,139:4:0 17 2960 . G 0 . DP=21;I16=11,10,0,0,778,29502,0,0,1260,75600,0,0,405,9109,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,178:6:0 0,12,126:4:0 17 2961 . G 0 . DP=20;I16=10,9,0,0,657,23303,0,0,1140,68400,0,0,380,8446,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,231:10:0 0,18,176:6:0 0,9,111:3:0 17 2962 . C 0 . DP=21;I16=10,10,0,0,731,27459,0,0,1200,72000,0,0,385,8615,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,151:5:0 0,12,132:4:0 17 2963 . C 0 . DP=21;I16=10,10,0,0,722,26502,0,0,1200,72000,0,0,378,8274,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,169:6:0 0,9,100:3:0 17 2964 . C 0 . DP=22;I16=11,10,0,0,752,27740,0,0,1260,75600,0,0,379,8275,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,148:5:0 0,15,151:5:0 17 2965 . A 0 . DP=22;I16=11,11,0,0,804,29606,0,0,1320,79200,0,0,397,8539,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,176:6:0 0,15,155:5:0 17 2966 . C 0 . DP=22;I16=11,11,0,0,705,23557,0,0,1320,79200,0,0,396,8468,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,254:11:0 0,18,146:6:0 0,15,142:5:0 17 2967 . G 0 . DP=24;I16=11,13,0,0,767,25405,0,0,1440,86400,0,0,393,8325,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,162:6:0 0,18,135:6:0 17 2968 . G 0 . DP=23;I16=11,12,0,0,812,29824,0,0,1380,82800,0,0,393,8213,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,152:5:0 0,18,162:6:0 17 2969 . C 0 . DP=23;I16=11,12,0,0,866,32982,0,0,1380,82800,0,0,393,8133,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,151:5:0 0,18,181:6:0 17 2970 . T 0 . DP=24;I16=11,12,0,0,816,29806,0,0,1380,82800,0,0,393,8085,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,154:5:0 0,18,189:6:0 17 2971 . G 0 . DP=24;I16=12,12,0,0,878,33010,0,0,1440,86400,0,0,392,7970,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,173:6:0 0,18,184:6:0 17 2972 . G 0 . DP=24;I16=12,12,0,0,839,30363,0,0,1440,86400,0,0,391,7889,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,175:6:0 0,18,161:6:0 17 2973 . C 0 . DP=24;I16=12,12,0,0,854,31154,0,0,1440,86400,0,0,390,7842,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,177:6:0 0,18,165:6:0 17 2974 . A 0 . DP=24;I16=12,12,0,0,869,32231,0,0,1440,86400,0,0,388,7778,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,169:6:0 0,18,174:6:0 17 2975 . G 0 . DP=24;I16=12,12,0,0,857,31835,0,0,1440,86400,0,0,384,7648,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,173:6:0 0,18,167:6:0 17 2976 . G 0 . DP=25;I16=13,12,0,0,866,31118,0,0,1500,90000,0,0,380,7554,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,180:7:0 0,18,171:6:0 17 2977 . T 0 . DP=26;I16=13,12,0,0,760,25216,0,0,1469,87241,0,0,373,7437,0,0;QS=3,0;MQSB=0.948139;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,151:6:0 0,18,155:6:0 17 2978 . G 0 . DP=25;I16=12,12,0,0,862,31574,0,0,1409,83641,0,0,362,7290,0,0;QS=3,0;MQSB=0.941765;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,168:6:0 0,18,167:6:0 17 2979 . G 0 . DP=24;I16=12,12,0,0,816,28440,0,0,1409,83641,0,0,370,7340,0,0;QS=3,0;MQSB=0.941765;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,164:6:0 0,15,142:5:0 17 2980 . G 0 . DP=23;I16=12,11,0,0,834,30882,0,0,1349,80041,0,0,369,7293,0,0;QS=3,0;MQSB=0.944319;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,144:5:0 0,15,148:5:0 17 2981 . A 0 . DP=23;I16=9,11,0,0,622,20306,0,0,1169,69241,0,0,318,6244,0,0;QS=3,0;MQSB=0.916401;MQ0F=0 PL:DP:DV 0,30,201:10:0 0,15,145:5:0 0,15,150:5:0 17 2982 . C 0 . DP=23;I16=10,11,0,0,706,24366,0,0,1229,72841,0,0,340,6884,0,0;QS=3,0;MQSB=0.927743;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,93:3:0 0,15,144:5:0 17 2983 . C 0 . DP=23;I16=12,11,0,0,781,27427,0,0,1349,80041,0,0,363,7197,0,0;QS=3,0;MQSB=0.944319;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,137:5:0 0,15,142:5:0 17 2984 . C 0 . DP=23;I16=12,11,0,0,861,32655,0,0,1349,80041,0,0,361,7229,0,0;QS=3,0;MQSB=0.944319;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,154:5:0 0,15,157:5:0 17 2985 . A 0 . DP=23;I16=12,11,0,0,798,28906,0,0,1349,80041,0,0,359,7293,0,0;QS=3,0;MQSB=0.944319;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,161:5:0 0,15,151:5:0 17 2986 . G 0 . DP=21;I16=11,10,0,0,701,24335,0,0,1229,72841,0,0,358,7388,0,0;QS=3,0;MQSB=0.939898;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,129:5:0 0,12,126:4:0 17 2987 . C 0 . DP=21;I16=10,10,0,0,720,26782,0,0,1169,69241,0,0,350,7448,0,0;QS=3,0;MQSB=0.931063;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,136:4:0 0,12,132:4:0 17 2988 . T 0 . DP=20;I16=10,10,0,0,693,25143,0,0,1169,69241,0,0,358,7612,0,0;QS=3,0;MQSB=0.931063;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,132:5:0 0,12,136:4:0 17 2989 . G 0 . DP=20;I16=10,10,0,0,708,25886,0,0,1169,69241,0,0,358,7736,0,0;QS=3,0;MQSB=0.931063;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,141:5:0 0,12,125:4:0 17 2990 . C 0 . DP=20;I16=10,10,0,0,697,25083,0,0,1169,69241,0,0,357,7833,0,0;QS=3,0;MQSB=0.931063;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,149:5:0 0,12,129:4:0 17 2991 . A 0 . DP=20;I16=10,10,0,0,717,26309,0,0,1169,69241,0,0,356,7952,0,0;QS=3,0;MQSB=0.931063;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,151:5:0 0,12,131:4:0 17 2992 . G 0 . DP=18;I16=10,8,0,0,625,22505,0,0,1049,62041,0,0,356,8042,0,0;QS=3,0;MQSB=0.938795;MQ0F=0 PL:DP:DV 0,30,243:10:0 0,12,130:4:0 0,12,120:4:0 17 2993 . G 0 . DP=18;I16=10,8,0,0,646,23620,0,0,1049,62041,0,0,354,8050,0,0;QS=3,0;MQSB=0.938795;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,118:4:0 0,12,123:4:0 17 2994 . G 0 . DP=18;I16=10,8,0,0,620,21828,0,0,1049,62041,0,0,351,8025,0,0;QS=3,0;MQSB=0.938795;MQ0F=0 PL:DP:DV 0,30,254:10:0 0,12,115:4:0 0,12,116:4:0 17 2995 . G 0 . DP=18;I16=10,8,0,0,628,22284,0,0,1049,62041,0,0,348,8018,0,0;QS=3,0;MQSB=0.938795;MQ0F=0 PL:DP:DV 0,30,248:10:0 0,12,110:4:0 0,12,135:4:0 17 2996 . T 0 . DP=17;I16=8,8,0,0,497,16697,0,0,929,54841,0,0,323,7493,0,0;QS=3,0;MQSB=0.915545;MQ0F=0 PL:DP:DV 0,30,215:10:0 0,9,75:3:0 0,9,107:3:0 17 2997 . C 0 . DP=17;I16=9,8,0,0,603,21925,0,0,989,58441,0,0,341,7901,0,0;QS=3,0;MQSB=0.928603;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,85:3:0 0,12,115:4:0 17 2998 . C 0 . DP=17;I16=9,8,0,0,576,19964,0,0,989,58441,0,0,337,7841,0,0;QS=3,0;MQSB=0.928603;MQ0F=0 PL:DP:DV 0,30,247:10:0 0,9,84:3:0 0,12,123:4:0 17 2999 . A 0 . DP=17;I16=9,8,0,0,599,22077,0,0,989,58441,0,0,333,7797,0,0;QS=3,0;MQSB=0.928603;MQ0F=0 PL:DP:DV 0,30,238:10:0 0,9,109:3:0 0,12,136:4:0 17 3000 . G 0 . DP=15;I16=8,7,0,0,554,20620,0,0,869,51241,0,0,331,7767,0,0;QS=3,0;MQSB=0.921243;MQ0F=0 PL:DP:DV 0,24,220:8:0 0,9,102:3:0 0,12,135:4:0 17 3001 . C 0 . DP=15;I16=7,7,0,0,521,19695,0,0,809,47641,0,0,309,7349,0,0;QS=3,0;MQSB=0.885987;MQ0F=0 PL:DP:DV 0,21,213:7:0 0,9,103:3:0 0,12,128:4:0 17 3002 . A 0 . DP=15;I16=8,7,0,0,542,20082,0,0,869,51241,0,0,326,7692,0,0;QS=3,0;MQSB=0.921243;MQ0F=0 PL:DP:DV 0,24,224:8:0 0,9,92:3:0 0,12,139:4:0 17 3003 . G 0 . DP=15;I16=8,7,0,0,540,19814,0,0,869,51241,0,0,322,7594,0,0;QS=3,0;MQSB=0.921243;MQ0F=0 PL:DP:DV 0,24,230:8:0 0,9,96:3:0 0,12,120:4:0 17 3004 . C 0 . DP=15;I16=8,7,0,0,525,19113,0,0,869,51241,0,0,318,7504,0,0;QS=3,0;MQSB=0.921243;MQ0F=0 PL:DP:DV 0,24,211:8:0 0,9,100:3:0 0,12,129:4:0 17 3005 . A 0 . DP=14;I16=7,7,0,0,491,17343,0,0,809,47641,0,0,315,7421,0,0;QS=3,0;MQSB=0.885987;MQ0F=0 PL:DP:DV 0,21,197:7:0 0,9,95:3:0 0,12,124:4:0 17 3006 . C 0 . DP=16;I16=7,8,0,0,516,18376,0,0,869,51241,0,0,312,7344,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,24,200:8:0 0,9,95:3:0 0,12,135:4:0 17 3007 . C 0 . DP=16;I16=7,8,0,0,553,20853,0,0,869,51241,0,0,310,7274,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,24,214:8:0 0,9,103:3:0 0,12,144:4:0 17 3008 . C 0 . DP=16;I16=7,8,0,0,576,22238,0,0,869,51241,0,0,308,7212,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,24,228:8:0 0,9,105:3:0 0,12,145:4:0 17 3009 . A 0 . DP=16;I16=7,8,0,0,493,16739,0,0,869,51241,0,0,306,7158,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,24,200:8:0 0,9,83:3:0 0,12,130:4:0 17 3010 . C 0 . DP=15;I16=7,7,0,0,534,20464,0,0,809,47641,0,0,300,7096,0,0;QS=3,0;MQSB=0.885987;MQ0F=0 PL:DP:DV 0,21,211:7:0 0,9,106:3:0 0,12,135:4:0 17 3011 . A 0 . DP=16;I16=7,8,0,0,559,21173,0,0,869,51241,0,0,302,7074,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,24,218:8:0 0,9,104:3:0 0,12,145:4:0 17 3012 . G 0 . DP=16;I16=7,8,0,0,503,17903,0,0,869,51241,0,0,300,7044,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,24,193:8:0 0,9,93:3:0 0,12,141:4:0 17 3013 . C 0 . DP=16;I16=7,9,0,0,549,19967,0,0,898,52082,0,0,305,7071,0,0;QS=3,0;MQSB=0.994413;MQ0F=0 PL:DP:DV 0,27,207:9:0 0,9,98:3:0 0,12,141:4:0 17 3014 . A 0 . DP=18;I16=6,9,0,0,542,20362,0,0,838,48482,0,0,289,6959,0,0;QS=3,0;MQSB=0.984496;MQ0F=0 PL:DP:DV 0,24,195:8:0 0,9,109:3:0 0,12,141:4:0 17 3015 . G 0 . DP=20;I16=7,9,0,0,549,19441,0,0,929,54841,0,0,311,7547,0,0;QS=3,0;MQSB=0.892753;MQ0F=0 PL:DP:DV 0,24,206:8:0 0,12,116:4:0 0,12,131:4:0 17 3016 . C 0 . DP=20;I16=7,13,0,0,690,24374,0,0,1138,66482,0,0,337,7783,0,0;QS=3,0;MQSB=0.972138;MQ0F=0 PL:DP:DV 0,33,248:11:0 0,15,136:5:0 0,12,132:4:0 17 3017 . C A, 0 . DP=20;I16=7,12,0,1,650,23264,23,529,1109,65641,29,841,329,7715,11,121;QS=2.93801,0.0619946,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.972138;BQB=1;MQ0F=0 PL:DP:DV 0,10,224,30,227,236:11:1 0,15,133,15,133,133:5:0 0,12,128,12,128,128:4:0 17 3018 . A 0 . DP=21;I16=6,10,0,0,475,15057,0,0,929,54841,0,0,295,6991,0,0;QS=3,0;MQSB=0.863243;MQ0F=0 PL:DP:DV 0,27,192:9:0 0,12,111:4:0 0,9,81:3:0 17 3019 . C 0 . DP=20;I16=6,12,0,0,618,21842,0,0,1049,62041,0,0,336,7818,0,0;QS=3,0;MQSB=0.85394;MQ0F=0 PL:DP:DV 0,30,242:10:0 0,15,129:5:0 0,9,104:3:0 17 3020 . C 0 . DP=20;I16=6,12,0,0,588,21046,0,0,1049,62041,0,0,340,7888,0,0;QS=3,0;MQSB=0.85394;MQ0F=0 PL:DP:DV 0,30,221:10:0 0,15,129:5:0 0,9,114:3:0 17 3021 . T 0 . DP=21;I16=5,13,0,0,618,22454,0,0,1049,62041,0,0,343,7921,0,0;QS=3,0;MQSB=0.814433;MQ0F=0 PL:DP:DV 0,30,216:10:0 0,15,147:5:0 0,9,109:3:0 17 3022 . G 0 . DP=20;I16=5,13,0,0,672,25292,0,0,1049,62041,0,0,348,7968,0,0;QS=3,0;MQSB=0.814433;MQ0F=0 PL:DP:DV 0,30,234:10:0 0,15,153:5:0 0,9,109:3:0 17 3023 . T G, 0 . DP=20;I16=5,12,0,1,554,19110,18,324,989,58441,60,3600,336,7740,17,289;QS=2.94231,0.0576923,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.814433;BQB=1;MQ0F=0 PL:DP:DV 0,12,191,27,194,199:10:1 0,15,125,15,125,125:5:0 0,9,111,9,111,111:3:0 17 3024 . G 0 . DP=20;I16=5,14,0,0,684,25122,0,0,1109,65641,0,0,382,8680,0,0;QS=3,0;MQSB=0.810584;MQ0F=0 PL:DP:DV 0,30,235:10:0 0,18,152:6:0 0,9,113:3:0 17 3025 . G 0 . DP=20;I16=5,14,0,0,625,21465,0,0,1109,65641,0,0,386,8722,0,0;QS=3,0;MQSB=0.810584;MQ0F=0 PL:DP:DV 0,30,225:10:0 0,18,133:6:0 0,9,109:3:0 17 3026 . C 0 . DP=20;I16=5,13,0,0,632,23132,0,0,1018,59282,0,0,367,8217,0,0;QS=3,0;MQSB=0.925212;MQ0F=0 PL:DP:DV 0,33,235:11:0 0,12,105:4:0 0,9,107:3:0 17 3027 . A 0 . DP=21;I16=6,15,0,0,693,24199,0,0,1198,70082,0,0,413,9199,0,0;QS=3,0;MQSB=0.940481;MQ0F=0 PL:DP:DV 0,33,222:11:0 0,21,175:7:0 0,9,108:3:0 17 3028 . G 0 . DP=21;I16=6,15,0,0,753,27935,0,0,1198,70082,0,0,417,9239,0,0;QS=3,0;MQSB=0.940481;MQ0F=0 PL:DP:DV 0,33,241:11:0 0,21,192:7:0 0,9,107:3:0 17 3029 . G 0 . DP=21;I16=6,15,0,0,787,29949,0,0,1198,70082,0,0,421,9303,0,0;QS=3,0;MQSB=0.940481;MQ0F=0 PL:DP:DV 0,33,251:11:0 0,21,189:7:0 0,9,109:3:0 17 3030 . G 0 . DP=22;I16=6,16,0,0,720,24728,0,0,1258,73682,0,0,424,9342,0,0;QS=3,0;MQSB=0.934321;MQ0F=0 PL:DP:DV 0,36,229:12:0 0,21,174:7:0 0,9,106:3:0 17 3031 . A 0 . DP=22;I16=5,16,0,0,693,24631,0,0,1198,70082,0,0,403,8783,0,0;QS=3,0;MQSB=0.902014;MQ0F=0 PL:DP:DV 0,36,222:12:0 0,18,162:6:0 0,9,113:3:0 17 3032 . G 0 . DP=22;I16=6,15,0,0,727,26093,0,0,1229,72841,0,0,405,8775,0,0;QS=3,0;MQSB=0.843281;MQ0F=0 PL:DP:DV 0,33,237:11:0 0,21,180:7:0 0,9,106:3:0 17 3033 . G 0 . DP=22;I16=6,15,0,0,697,24039,0,0,1198,70082,0,0,429,9407,0,0;QS=3,0;MQSB=0.940481;MQ0F=0 PL:DP:DV 0,33,235:11:0 0,21,160:7:0 0,9,106:3:0 17 3034 . A 0 . DP=23;I16=6,14,0,0,617,20947,0,0,1138,66482,0,0,387,8491,0,0;QS=3,0;MQSB=0.947033;MQ0F=0 PL:DP:DV 0,33,203:11:0 0,15,134:5:0 0,12,126:4:0 17 3035 . G 0 . DP=23;I16=7,13,0,0,641,22007,0,0,1138,66482,0,0,385,8379,0,0;QS=3,0;MQSB=0.972138;MQ0F=0 PL:DP:DV 0,30,208:10:0 0,18,151:6:0 0,12,128:4:0 17 3036 . C 0 . DP=23;I16=6,15,0,0,688,23652,0,0,1198,70082,0,0,388,8258,0,0;QS=3,0;MQSB=0.940481;MQ0F=0 PL:DP:DV 0,36,216:12:0 0,15,132:5:0 0,12,140:4:0 17 3037 . T 0 . DP=24;I16=7,16,0,0,797,28671,0,0,1318,77282,0,0,439,9521,0,0;QS=3,0;MQSB=0.955805;MQ0F=0 PL:DP:DV 0,36,242:12:0 0,21,170:7:0 0,12,143:4:0 17 3038 . T 0 . DP=25;I16=8,17,0,0,815,27469,0,0,1438,84482,0,0,441,9561,0,0;QS=3,0;MQSB=0.966223;MQ0F=0 PL:DP:DV 0,42,249:14:0 0,21,190:7:0 0,12,125:4:0 17 3039 . G 0 . DP=25;I16=8,17,0,0,780,25946,0,0,1438,84482,0,0,444,9630,0,0;QS=3,0;MQSB=0.966223;MQ0F=0 PL:DP:DV 0,42,249:14:0 0,21,156:7:0 0,12,127:4:0 17 3040 . T 0 . DP=25;I16=7,15,0,0,701,23777,0,0,1258,73682,0,0,385,8279,0,0;QS=3,0;MQSB=0.961028;MQ0F=0 PL:DP:DV 0,36,239:12:0 0,18,154:6:0 0,12,130:4:0 17 3041 . G 0 . DP=25;I16=8,16,0,0,824,29228,0,0,1378,80882,0,0,420,8982,0,0;QS=3,0;MQSB=0.970446;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,186:7:0 0,12,135:4:0 17 3042 . G 0 . DP=25;I16=8,15,0,0,795,28227,0,0,1349,80041,0,0,409,8839,0,0;QS=3,0;MQSB=0.889418;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,148:6:0 0,12,120:4:0 17 3043 . T 0 . DP=25;I16=8,16,0,0,723,23191,0,0,1378,80882,0,0,435,9415,0,0;QS=3,0;MQSB=0.970446;MQ0F=0 PL:DP:DV 0,39,233:13:0 0,21,151:7:0 0,12,124:4:0 17 3044 . A C, 0 . DP=26;I16=8,15,0,1,665,20809,15,225,1318,77282,60,3600,392,8498,14,196;QS=2.96104,0.038961,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.970446;BQB=1;MQ0F=0 PL:DP:DV 0,26,216,39,219,220:14:1 0,18,133,18,133,133:6:0 0,12,124,12,124,124:4:0 17 3045 . C 0 . DP=26;I16=8,16,0,0,754,25232,0,0,1378,80882,0,0,403,8627,0,0;QS=3,0;MQSB=0.970446;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,133:6:0 0,12,122:4:0 17 3046 . A 0 . DP=25;I16=8,15,0,0,748,26170,0,0,1349,80041,0,0,400,8540,0,0;QS=3,0;MQSB=0.889418;MQ0F=0 PL:DP:DV 0,39,245:13:0 0,18,160:6:0 0,12,141:4:0 17 3047 . G 0 . DP=25;I16=8,15,0,0,766,26998,0,0,1318,77282,0,0,396,8432,0,0;QS=3,0;MQSB=0.974802;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,143:6:0 0,12,129:4:0 17 3048 . T 0 . DP=25;I16=8,11,0,0,608,20554,0,0,1109,65641,0,0,320,6762,0,0;QS=3,0;MQSB=0.902014;MQ0F=0 PL:DP:DV 0,30,226:10:0 0,15,128:5:0 0,12,129:4:0 17 3049 . G 0 . DP=24;I16=8,16,0,0,831,29557,0,0,1378,80882,0,0,426,9068,0,0;QS=3,0;MQSB=0.970446;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,161:6:0 0,9,105:3:0 17 3050 . G 0 . DP=24;I16=8,14,0,0,729,25093,0,0,1258,73682,0,0,379,8041,0,0;QS=3,0;MQSB=0.979255;MQ0F=0 PL:DP:DV 0,39,253:13:0 0,18,159:6:0 0,9,100:3:0 17 3051 . A 0 . DP=23;I16=8,15,0,0,724,24200,0,0,1318,77282,0,0,423,9091,0,0;QS=3,0;MQSB=0.974802;MQ0F=0 PL:DP:DV 0,42,243:14:0 0,18,152:6:0 0,9,103:3:0 17 3052 . C 0 . DP=23;I16=7,13,0,0,612,19876,0,0,1169,69241,0,0,363,7779,0,0;QS=3,0;MQSB=0.962269;MQ0F=0 PL:DP:DV 0,36,226:12:0 0,15,117:5:0 0,9,93:3:0 17 3053 . A 0 . DP=22;I16=8,11,0,0,616,21288,0,0,1078,62882,0,0,360,7740,0,0;QS=3,0;MQSB=0.992359;MQ0F=0 PL:DP:DV 0,33,234:11:0 0,15,118:5:0 0,9,110:3:0 17 3054 . G 0 . DP=22;I16=8,14,0,0,761,27617,0,0,1258,73682,0,0,414,8932,0,0;QS=3,0;MQSB=0.979255;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,126:5:0 0,9,99:3:0 17 3055 . G 0 . DP=22;I16=8,9,0,0,566,19890,0,0,958,55682,0,0,333,7219,0,0;QS=3,0;MQSB=0.998843;MQ0F=0 PL:DP:DV 0,30,228:10:0 0,12,111:4:0 0,9,105:3:0 17 3056 . C 0 . DP=22;I16=7,14,0,0,701,24685,0,0,1198,70082,0,0,420,9298,0,0;QS=3,0;MQSB=0.966484;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,125:4:0 0,12,122:4:0 17 3057 . C 0 . DP=23;I16=8,13,0,0,706,24988,0,0,1198,70082,0,0,411,9253,0,0;QS=3,0;MQSB=0.983744;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,128:5:0 0,9,92:3:0 17 3058 . C 0 . DP=23;I16=9,14,0,0,707,23327,0,0,1318,77282,0,0,429,9471,0,0;QS=3,0;MQSB=0.987676;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,131:5:0 0,12,116:4:0 17 3059 . T G, 0 . DP=23;I16=9,11,0,1,638,21954,16,256,1169,69241,60,3600,355,7761,22,484;QS=2.95876,0.0412371,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.913101;BQB=1;MQ0F=0 PL:DP:DV 0,22,234,36,237,239:13:1 0,15,144,15,144,144:5:0 0,9,94,9,94,94:3:0 17 3060 . G 0 . DP=24;I16=9,10,0,0,578,19136,0,0,1109,65641,0,0,324,6992,0,0;QS=3,0;MQSB=0.920044;MQ0F=0 PL:DP:DV 0,33,221:11:0 0,12,121:4:0 0,12,116:4:0 17 3061 . C 0 . DP=24;I16=9,15,0,0,770,25918,0,0,1378,80882,0,0,446,10136,0,0;QS=3,0;MQSB=0.984127;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,138:5:0 0,15,145:5:0 17 3062 . C 0 . DP=23;I16=9,13,0,0,727,25019,0,0,1289,76441,0,0,419,9551,0,0;QS=3,0;MQSB=0.910098;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,137:5:0 0,15,134:5:0 17 3063 . C 0 . DP=23;I16=9,13,0,0,651,21695,0,0,1258,73682,0,0,415,9511,0,0;QS=3,0;MQSB=0.991121;MQ0F=0 PL:DP:DV 0,36,233:12:0 0,15,130:5:0 0,15,133:5:0 17 3064 . A 0 . DP=23;I16=9,12,0,0,691,24125,0,0,1198,70082,0,0,385,8815,0,0;QS=3,0;MQSB=0.994334;MQ0F=0 PL:DP:DV 0,36,254:12:0 0,12,118:4:0 0,15,144:5:0 17 3065 . G 0 . DP=22;I16=7,14,0,0,653,21733,0,0,1198,70082,0,0,426,9986,0,0;QS=3,0;MQSB=0.966484;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,109:4:0 0,12,118:4:0 17 3066 . A 0 . DP=21;I16=7,10,0,0,501,16143,0,0,989,58441,0,0,326,7598,0,0;QS=3,0;MQSB=0.887766;MQ0F=0 PL:DP:DV 0,27,202:9:0 0,12,109:4:0 0,12,104:4:0 17 3067 . T 0 . DP=21;I16=7,12,0,0,535,16779,0,0,1078,62882,0,0,373,8787,0,0;QS=3,0;MQSB=0.977926;MQ0F=0 PL:DP:DV 0,36,223:12:0 0,9,82:3:0 0,12,103:4:0 17 3068 . G 0 . DP=20;I16=7,12,0,0,645,22781,0,0,1109,65641,0,0,396,9312,0,0;QS=3,0;MQSB=0.879351;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,115:4:0 0,9,84:3:0 17 3069 . G 0 . DP=20;I16=7,10,0,0,468,14412,0,0,989,58441,0,0,346,8070,0,0;QS=3,0;MQSB=0.887766;MQ0F=0 PL:DP:DV 0,33,203:11:0 0,12,102:4:0 0,6,57:2:0 17 3070 . C 0 . DP=20;I16=6,13,0,0,627,22135,0,0,1078,62882,0,0,414,9878,0,0;QS=3,0;MQSB=0.953977;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,98:3:0 0,9,98:3:0 17 3071 . C 0 . DP=20;I16=7,13,0,0,715,26563,0,0,1138,66482,0,0,419,9893,0,0;QS=3,0;MQSB=0.972138;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,123:4:0 0,9,98:3:0 17 3072 . C 0 . DP=20;I16=6,13,0,0,696,25784,0,0,1109,65641,0,0,414,9866,0,0;QS=3,0;MQSB=0.965977;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,128:4:0 0,9,106:3:0 17 3073 . C 0 . DP=20;I16=6,13,0,0,706,26822,0,0,1109,65641,0,0,414,9872,0,0;QS=3,0;MQSB=0.965977;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,135:4:0 0,9,115:3:0 17 3074 . C 0 . DP=20;I16=6,13,0,0,724,28052,0,0,1109,65641,0,0,414,9886,0,0;QS=3,0;MQSB=0.965977;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,135:4:0 0,9,116:3:0 17 3075 . C 0 . DP=21;I16=6,13,0,0,630,21614,0,0,1109,65641,0,0,389,9283,0,0;QS=3,0;MQSB=0.965977;MQ0F=0 PL:DP:DV 0,33,239:11:0 0,15,140:5:0 0,9,94:3:0 17 3076 . G 0 . DP=21;I16=6,14,0,0,645,21809,0,0,1169,69241,0,0,415,9939,0,0;QS=3,0;MQSB=0.969852;MQ0F=0 PL:DP:DV 0,36,252:12:0 0,15,126:5:0 0,9,91:3:0 17 3077 . C 0 . DP=20;I16=5,15,0,0,694,25228,0,0,1169,69241,0,0,417,9979,0,0;QS=3,0;MQSB=0.976472;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,116:4:0 0,9,111:3:0 17 3078 . C 0 . DP=20;I16=5,15,0,0,767,29761,0,0,1169,69241,0,0,420,10028,0,0;QS=3,0;MQSB=0.976472;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,113:4:0 0,9,111:3:0 17 3079 . T 0 . DP=20;I16=5,15,0,0,749,28479,0,0,1169,69241,0,0,422,10038,0,0;QS=3,0;MQSB=0.976472;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,119:4:0 0,9,116:3:0 17 3080 . G 0 . DP=21;I16=6,15,0,0,785,29901,0,0,1229,72841,0,0,424,10060,0,0;QS=3,0;MQSB=0.973096;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,124:4:0 0,9,103:3:0 17 3081 . C 0 . DP=21;I16=6,15,0,0,752,27438,0,0,1229,72841,0,0,425,9997,0,0;QS=3,0;MQSB=0.973096;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,118:4:0 0,9,92:3:0 17 3082 . C 0 . DP=21;I16=6,15,0,0,787,30211,0,0,1229,72841,0,0,426,9952,0,0;QS=3,0;MQSB=0.973096;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,118:4:0 0,9,112:3:0 17 3083 . T C, 0 . DP=21;I16=6,14,0,1,764,29458,33,1089,1169,69241,60,3600,401,9249,25,625;QS=2.93666,0.0633397,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.973096;BQB=1;MQ0F=0 PL:DP:DV 0,9,255,39,255,255:14:1 0,12,130,12,130,130:4:0 0,9,113,9,113,113:3:0 17 3084 . G 0 . DP=23;I16=6,17,0,0,859,32755,0,0,1349,80041,0,0,426,9812,0,0;QS=3,0;MQSB=0.978183;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,118:4:0 0,12,135:4:0 17 3085 . T 0 . DP=23;I16=6,16,0,0,824,31132,0,0,1289,76441,0,0,426,9718,0,0;QS=3,0;MQSB=0.97584;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,124:4:0 0,12,135:4:0 17 3086 . G 0 . DP=23;I16=6,17,0,0,853,32429,0,0,1349,80041,0,0,428,9648,0,0;QS=3,0;MQSB=0.978183;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,125:4:0 0,12,131:4:0 17 3087 . G 0 . DP=23;I16=6,17,0,0,897,35253,0,0,1349,80041,0,0,429,9599,0,0;QS=3,0;MQSB=0.978183;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,130:4:0 0,12,137:4:0 17 3088 . A 0 . DP=22;I16=6,16,0,0,834,31738,0,0,1289,76441,0,0,431,9571,0,0;QS=3,0;MQSB=0.97584;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,110:3:0 0,12,135:4:0 17 3089 . A 0 . DP=23;I16=7,16,0,0,919,36903,0,0,1349,80041,0,0,432,9514,0,0;QS=3,0;MQSB=0.973027;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,113:3:0 0,15,175:5:0 17 3090 . G 0 . DP=23;I16=7,16,0,0,885,34349,0,0,1349,80041,0,0,433,9431,0,0;QS=3,0;MQSB=0.973027;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,103:3:0 0,15,160:5:0 17 3091 . T 0 . DP=23;I16=7,16,0,0,840,31352,0,0,1349,80041,0,0,434,9374,0,0;QS=3,0;MQSB=0.973027;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,105:3:0 0,15,153:5:0 17 3092 . T 0 . DP=24;I16=8,16,0,0,895,33673,0,0,1409,83641,0,0,434,9294,0,0;QS=3,0;MQSB=0.970446;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,109:3:0 0,15,165:5:0 17 3093 . G 0 . DP=25;I16=9,16,0,0,955,37033,0,0,1469,87241,0,0,434,9192,0,0;QS=3,0;MQSB=0.968069;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,133:4:0 0,15,157:5:0 17 3094 . A 0 . DP=25;I16=9,15,0,0,891,33319,0,0,1409,83641,0,0,425,9019,0,0;QS=3,0;MQSB=0.96464;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,134:4:0 0,15,161:5:0 17 3095 . C 0 . DP=25;I16=9,15,0,0,891,33469,0,0,1409,83641,0,0,425,8955,0,0;QS=3,0;MQSB=0.96464;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,131:4:0 0,15,163:5:0 17 3096 . C 0 . DP=25;I16=9,16,0,0,952,36626,0,0,1469,87241,0,0,436,9014,0,0;QS=3,0;MQSB=0.968069;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,121:4:0 0,15,167:5:0 17 3097 . A 0 . DP=25;I16=9,16,0,0,972,38200,0,0,1469,87241,0,0,436,8984,0,0;QS=3,0;MQSB=0.968069;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,144:4:0 0,15,174:5:0 17 3098 . G 0 . DP=25;I16=9,16,0,0,959,37269,0,0,1469,87241,0,0,436,8986,0,0;QS=3,0;MQSB=0.968069;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,134:4:0 0,15,166:5:0 17 3099 . A 0 . DP=25;I16=9,16,0,0,888,32632,0,0,1469,87241,0,0,435,8971,0,0;QS=3,0;MQSB=0.968069;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,124:4:0 0,15,165:5:0 17 3100 . C 0 . DP=25;I16=9,16,0,0,870,31084,0,0,1469,87241,0,0,434,8990,0,0;QS=3,0;MQSB=0.968069;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,126:4:0 0,15,159:5:0 17 3101 . C 0 . DP=25;I16=9,16,0,0,960,37090,0,0,1469,87241,0,0,432,8992,0,0;QS=3,0;MQSB=0.968069;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,134:4:0 0,15,161:5:0 17 3102 . A 0 . DP=26;I16=10,16,0,0,952,35186,0,0,1529,90841,0,0,430,9026,0,0;QS=3,0;MQSB=0.965874;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,134:4:0 0,18,189:6:0 17 3103 . T 0 . DP=26;I16=10,16,0,0,920,33094,0,0,1529,90841,0,0,427,8993,0,0;QS=3,0;MQSB=0.965874;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,131:4:0 0,18,187:6:0 17 3104 . C T, 0 . DP=25;I16=8,15,2,0,900,35584,80,3202,1349,80041,120,7200,385,8143,40,850;QS=2.58763,0.412371,0;VDB=0.8;SGB=0.346553;RPB=0.717391;MQB=0.956522;MQSB=0.962269;BQB=0.978261;MQ0F=0 PL:DP:DV 0,48,255,48,255,255:16:0 0,12,144,12,144,144:4:0 59,0,93,68,99,157:5:2 17 3105 . T 0 . DP=25;I16=10,15,0,0,959,37057,0,0,1469,87241,0,0,422,8976,0,0;QS=3,0;MQSB=0.962269;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,12,149:4:0 0,15,169:5:0 17 3106 . G 0 . DP=23;I16=10,13,0,0,881,33891,0,0,1380,82800,0,0,420,8940,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,132:4:0 0,15,167:5:0 17 3107 . T 0 . DP=24;I16=10,14,0,0,878,32496,0,0,1440,86400,0,0,418,8932,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,132:4:0 0,18,195:6:0 17 3108 . C 0 . DP=24;I16=10,14,0,0,909,34897,0,0,1440,86400,0,0,417,8953,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,137:4:0 0,18,189:6:0 17 3109 . A 0 . DP=24;I16=10,14,0,0,905,34249,0,0,1440,86400,0,0,416,9004,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,136:4:0 0,18,195:6:0 17 3110 . C 0 . DP=24;I16=10,13,0,0,919,37099,0,0,1380,82800,0,0,413,8933,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,105:3:0 0,18,189:6:0 17 3111 . A 0 . DP=25;I16=10,14,0,0,961,38943,0,0,1440,86400,0,0,410,8888,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,111:3:0 0,18,205:6:0 17 3112 . G 0 . DP=25;I16=10,14,0,0,958,39370,0,0,1440,86400,0,0,407,8821,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,116:3:0 0,18,191:6:0 17 3113 . C 0 . DP=25;I16=10,14,0,0,961,39641,0,0,1440,86400,0,0,403,8735,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,112:3:0 0,18,193:6:0 17 3114 . A 0 . DP=24;I16=10,13,0,0,928,38448,0,0,1380,82800,0,0,400,8680,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,116:3:0 0,18,200:6:0 17 3115 . G 0 . DP=23;I16=10,12,0,0,872,35800,0,0,1320,79200,0,0,397,8603,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,117:3:0 0,18,195:6:0 17 3116 . G 0 . DP=23;I16=10,12,0,0,870,35372,0,0,1320,79200,0,0,394,8552,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,108:3:0 0,18,194:6:0 17 3117 . T 0 . DP=22;I16=9,13,0,0,771,27537,0,0,1320,79200,0,0,399,8575,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,96:3:0 0,18,177:6:0 17 3118 . A 0 . DP=22;I16=9,13,0,0,813,30285,0,0,1320,79200,0,0,397,8537,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,111:3:0 0,18,193:6:0 17 3119 . A 0 . DP=22;I16=9,13,0,0,886,35954,0,0,1320,79200,0,0,393,8423,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,120:3:0 0,18,207:6:0 17 3120 . G 0 . DP=22;I16=9,13,0,0,839,32281,0,0,1320,79200,0,0,389,8333,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,104:3:0 0,18,182:6:0 17 3121 . A 0 . DP=22;I16=10,12,0,0,777,28399,0,0,1320,79200,0,0,386,8266,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,111:3:0 0,18,194:6:0 17 3122 . C 0 . DP=22;I16=10,12,0,0,848,33002,0,0,1320,79200,0,0,384,8222,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,110:3:0 0,18,192:6:0 17 3123 . T 0 . DP=22;I16=10,12,0,0,854,33456,0,0,1320,79200,0,0,382,8202,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,114:3:0 0,18,204:6:0 17 3124 . C 0 . DP=22;I16=10,11,0,0,882,38286,0,0,1260,75600,0,0,381,8205,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,104:3:0 0,15,178:5:0 17 3125 . T 0 . DP=22;I16=10,11,0,0,860,36764,0,0,1260,75600,0,0,380,8230,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,106:3:0 0,15,185:5:0 17 3126 . G 0 . DP=22;I16=10,11,0,0,834,34998,0,0,1260,75600,0,0,379,8277,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,97:3:0 0,15,174:5:0 17 3127 . C 0 . DP=23;I16=10,11,0,0,847,36381,0,0,1260,75600,0,0,378,8346,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,87:3:0 0,15,175:5:0 17 3128 . T 0 . DP=22;I16=9,12,0,0,850,35972,0,0,1229,72841,0,0,402,9010,0,0;QS=3,0;MQSB=0.950149;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,107:3:0 0,15,182:5:0 17 3129 . T 0 . DP=22;I16=9,12,0,0,809,32621,0,0,1229,72841,0,0,401,9067,0,0;QS=3,0;MQSB=0.950149;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,107:3:0 0,15,178:5:0 17 3130 . T 0 . DP=21;I16=9,10,0,0,744,30322,0,0,1140,68400,0,0,376,8516,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,82:2:0 0,15,169:5:0 17 3131 . C 0 . DP=21;I16=9,10,0,0,792,34778,0,0,1140,68400,0,0,376,8606,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,6,83:2:0 0,15,170:5:0 17 3132 . T 0 . DP=21;I16=9,11,0,0,819,35197,0,0,1169,69241,0,0,400,9288,0,0;QS=3,0;MQSB=0.943233;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,109:3:0 0,15,174:5:0 17 3133 . G 0 . DP=21;I16=9,11,0,0,800,34016,0,0,1169,69241,0,0,398,9312,0,0;QS=3,0;MQSB=0.943233;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,97:3:0 0,15,166:5:0 17 3134 . G 0 . DP=22;I16=10,11,0,0,773,30227,0,0,1229,72841,0,0,396,9352,0,0;QS=3,0;MQSB=0.939898;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,97:3:0 0,15,163:5:0 17 3135 . G 0 . DP=22;I16=9,12,0,0,812,33714,0,0,1229,72841,0,0,396,9408,0,0;QS=3,0;MQSB=0.950149;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,94:3:0 0,12,151:4:0 17 3136 . C 0 . DP=22;I16=9,12,0,0,811,33469,0,0,1229,72841,0,0,396,9430,0,0;QS=3,0;MQSB=0.950149;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,102:3:0 0,12,140:4:0 17 3137 . A 0 . DP=22;I16=10,11,0,0,786,31226,0,0,1229,72841,0,0,396,9416,0,0;QS=3,0;MQSB=0.939898;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,95:3:0 0,12,142:4:0 17 3138 . A 0 . DP=21;I16=9,11,0,0,720,28200,0,0,1169,69241,0,0,398,9414,0,0;QS=3,0;MQSB=0.943233;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,98:3:0 0,12,147:4:0 17 3139 . C 0 . DP=21;I16=9,11,0,0,755,31433,0,0,1169,69241,0,0,400,9424,0,0;QS=3,0;MQSB=0.943233;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,91:3:0 0,12,139:4:0 17 3140 . C 0 . DP=21;I16=9,11,0,0,790,33238,0,0,1169,69241,0,0,402,9446,0,0;QS=3,0;MQSB=0.943233;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,102:3:0 0,12,145:4:0 17 3141 . C 0 . DP=21;I16=9,11,0,0,819,35401,0,0,1169,69241,0,0,404,9480,0,0;QS=3,0;MQSB=0.943233;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,101:3:0 0,12,150:4:0 17 3142 . A 0 . DP=21;I16=9,11,0,0,822,35744,0,0,1169,69241,0,0,405,9477,0,0;QS=3,0;MQSB=0.943233;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,107:3:0 0,12,155:4:0 17 3143 . G 0 . DP=22;I16=10,11,0,0,846,36468,0,0,1198,70082,0,0,406,9488,0,0;QS=3,0;MQSB=0.99938;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,104:3:0 0,12,151:4:0 17 3144 . C 0 . DP=23;I16=12,10,0,0,855,35905,0,0,1258,73682,0,0,409,9513,0,0;QS=3,0;MQSB=0.997828;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,104:3:0 0,15,165:5:0 17 3145 . A 0 . DP=23;I16=12,10,0,0,869,35937,0,0,1258,73682,0,0,414,9554,0,0;QS=3,0;MQSB=0.997828;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,106:3:0 0,15,174:5:0 17 3146 . G 0 . DP=23;I16=12,10,0,0,895,38345,0,0,1258,73682,0,0,419,9613,0,0;QS=3,0;MQSB=0.997828;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,105:3:0 0,15,179:5:0 17 3147 . G 0 . DP=24;I16=11,11,0,0,851,34815,0,0,1289,76441,0,0,419,9623,0,0;QS=3,0;MQSB=0.936864;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,123:4:0 0,15,172:5:0 17 3148 . T 0 . DP=24;I16=12,11,0,0,795,30097,0,0,1318,77282,0,0,428,9682,0,0;QS=3,0;MQSB=0.999527;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,107:4:0 0,15,149:5:0 17 3149 . G 0 . DP=24;I16=12,11,0,0,910,38142,0,0,1318,77282,0,0,433,9743,0,0;QS=3,0;MQSB=0.999527;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,120:4:0 0,15,167:5:0 17 3150 . A 0 . DP=24;I16=12,11,0,0,854,33784,0,0,1318,77282,0,0,438,9822,0,0;QS=3,0;MQSB=0.999527;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,129:4:0 0,15,167:5:0 17 3151 . C 0 . DP=24;I16=12,11,0,0,873,35315,0,0,1318,77282,0,0,442,9870,0,0;QS=3,0;MQSB=0.999527;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,125:4:0 0,15,163:5:0 17 3152 . C 0 . DP=24;I16=12,11,0,0,857,34239,0,0,1318,77282,0,0,445,9889,0,0;QS=3,0;MQSB=0.999527;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,123:4:0 0,15,155:5:0 17 3153 . C 0 . DP=24;I16=12,11,0,0,891,36711,0,0,1318,77282,0,0,448,9930,0,0;QS=3,0;MQSB=0.999527;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,125:4:0 0,15,166:5:0 17 3154 . T 0 . DP=25;I16=13,11,0,0,938,38052,0,0,1378,80882,0,0,451,9993,0,0;QS=3,0;MQSB=0.998323;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,133:4:0 0,18,194:6:0 17 3155 . G 0 . DP=25;I16=13,11,0,0,953,39423,0,0,1378,80882,0,0,454,10030,0,0;QS=3,0;MQSB=0.998323;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,127:4:0 0,18,180:6:0 17 3156 . G 0 . DP=25;I16=13,11,0,0,946,38818,0,0,1378,80882,0,0,457,10093,0,0;QS=3,0;MQSB=0.998323;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,126:4:0 0,18,189:6:0 17 3157 . A 0 . DP=24;I16=12,12,0,0,896,33648,0,0,1378,80882,0,0,486,10806,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,130:4:0 0,18,193:6:0 17 3158 . A 0 . DP=24;I16=12,12,0,0,856,31670,0,0,1378,80882,0,0,490,10918,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,125:4:0 0,18,192:6:0 17 3159 . T 0 . DP=24;I16=11,12,0,0,864,32952,0,0,1349,80041,0,0,477,10749,0,0;QS=3,0;MQSB=0.944319;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,134:4:0 0,18,194:6:0 17 3160 . T 0 . DP=24;I16=12,12,0,0,907,34719,0,0,1378,80882,0,0,494,11018,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,134:4:0 0,18,193:6:0 17 3161 . C 0 . DP=24;I16=12,12,0,0,880,33336,0,0,1378,80882,0,0,494,11006,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,123:4:0 0,18,196:6:0 17 3162 . C 0 . DP=24;I16=12,12,0,0,916,35764,0,0,1378,80882,0,0,494,11018,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,127:4:0 0,18,188:6:0 17 3163 . T A, 0 . DP=24;I16=11,12,1,0,895,35099,13,169,1349,80041,29,841,473,10603,20,400;QS=2.97436,0.025641,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0 PL:DP:DV 0,28,255,39,255,255:14:1 0,12,137,12,137,137:4:0 0,18,200,18,200,200:6:0 17 3164 . G 0 . DP=24;I16=12,11,0,0,887,34437,0,0,1349,80041,0,0,467,10385,0,0;QS=3,0;MQSB=0.944319;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,106:3:0 0,18,188:6:0 17 3165 . T 0 . DP=24;I16=12,12,0,0,880,32654,0,0,1378,80882,0,0,490,10990,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,133:4:0 0,18,189:6:0 17 3166 . C 0 . DP=24;I16=12,11,0,0,871,33389,0,0,1349,80041,0,0,463,10369,0,0;QS=3,0;MQSB=0.944319;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,111:3:0 0,18,193:6:0 17 3167 . C 0 . DP=23;I16=12,11,0,0,879,34199,0,0,1318,77282,0,0,487,11021,0,0;QS=3,0;MQSB=0.999527;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,131:4:0 0,18,189:6:0 17 3168 . A 0 . DP=23;I16=12,11,0,0,874,33294,0,0,1318,77282,0,0,486,11070,0,0;QS=3,0;MQSB=0.999527;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,125:4:0 0,18,192:6:0 17 3169 . T 0 . DP=23;I16=11,11,0,0,824,31124,0,0,1289,76441,0,0,458,10416,0,0;QS=3,0;MQSB=0.936864;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,128:4:0 0,18,187:6:0 17 3170 . C 0 . DP=23;I16=11,11,0,0,877,35167,0,0,1289,76441,0,0,453,10307,0,0;QS=3,0;MQSB=0.936864;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,129:4:0 0,18,201:6:0 17 3171 . T 0 . DP=23;I16=11,11,0,0,843,32615,0,0,1289,76441,0,0,448,10216,0,0;QS=3,0;MQSB=0.936864;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,127:4:0 0,18,201:6:0 17 3172 . G 0 . DP=23;I16=12,11,0,0,813,29605,0,0,1318,77282,0,0,468,10768,0,0;QS=3,0;MQSB=0.999527;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,120:4:0 0,18,176:6:0 17 3173 . G 0 . DP=23;I16=11,11,0,0,801,29659,0,0,1289,76441,0,0,436,9988,0,0;QS=3,0;MQSB=0.936864;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,126:4:0 0,18,174:6:0 17 3174 . C 0 . DP=24;I16=13,11,0,0,938,36926,0,0,1378,80882,0,0,454,10476,0,0;QS=3,0;MQSB=0.998323;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,127:4:0 0,18,194:6:0 17 3175 . A 0 . DP=25;I16=13,11,0,0,914,35230,0,0,1409,83641,0,0,422,9684,0,0;QS=3,0;MQSB=0.931547;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,127:4:0 0,21,209:7:0 17 3176 . G 0 . DP=24;I16=14,10,0,0,909,34913,0,0,1378,80882,0,0,442,10164,0,0;QS=3,0;MQSB=0.993166;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,97:3:0 0,21,205:7:0 17 3177 . G 0 . DP=23;I16=14,9,0,0,793,28159,0,0,1318,77282,0,0,438,10040,0,0;QS=3,0;MQSB=0.987676;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,9,93:3:0 0,21,195:7:0 17 3178 . T 0 . DP=23;I16=13,9,0,0,754,26290,0,0,1289,76441,0,0,408,9262,0,0;QS=3,0;MQSB=0.910098;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,96:3:0 0,21,173:7:0 17 3179 . G 0 . DP=23;I16=13,9,0,0,802,29686,0,0,1289,76441,0,0,403,9131,0,0;QS=3,0;MQSB=0.910098;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,97:3:0 0,21,188:7:0 17 3180 . G 0 . DP=22;I16=12,9,0,0,701,24107,0,0,1229,72841,0,0,398,8970,0,0;QS=3,0;MQSB=0.913101;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,92:3:0 0,21,175:7:0 17 3181 . G 0 . DP=22;I16=13,9,0,0,795,29503,0,0,1258,73682,0,0,418,9452,0,0;QS=3,0;MQSB=0.991121;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,99:3:0 0,21,193:7:0 17 3182 . C 0 . DP=22;I16=12,9,0,0,772,28878,0,0,1198,70082,0,0,396,9038,0,0;QS=3,0;MQSB=0.994334;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,99:3:0 0,21,184:7:0 17 3183 . A 0 . DP=22;I16=12,9,0,0,766,28304,0,0,1229,72841,0,0,382,8546,0,0;QS=3,0;MQSB=0.913101;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,106:3:0 0,21,193:7:0 17 3184 . T 0 . DP=21;I16=12,8,0,0,721,26533,0,0,1169,69241,0,0,377,8409,0,0;QS=3,0;MQSB=0.898397;MQ0F=0 PL:DP:DV 0,30,248:10:0 0,9,106:3:0 0,21,196:7:0 17 3185 . T 0 . DP=20;I16=13,7,0,0,740,27660,0,0,1138,66482,0,0,397,8865,0,0;QS=3,0;MQSB=0.972138;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,103:3:0 0,18,174:6:0 17 3186 . G 0 . DP=20;I16=13,7,0,0,741,28311,0,0,1138,66482,0,0,391,8665,0,0;QS=3,0;MQSB=0.972138;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,103:3:0 0,18,169:6:0 17 3187 . A 0 . DP=20;I16=13,7,0,0,693,24927,0,0,1138,66482,0,0,385,8485,0,0;QS=3,0;MQSB=0.972138;MQ0F=0 PL:DP:DV 0,33,247:11:0 0,9,102:3:0 0,18,171:6:0 17 3188 . A 0 . DP=20;I16=13,7,0,0,704,25746,0,0,1138,66482,0,0,379,8325,0,0;QS=3,0;MQSB=0.972138;MQ0F=0 PL:DP:DV 0,33,249:11:0 0,9,100:3:0 0,18,174:6:0 17 3189 . A 0 . DP=21;I16=13,8,0,0,763,28171,0,0,1198,70082,0,0,373,8185,0,0;QS=3,0;MQSB=0.983744;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,125:4:0 0,18,174:6:0 17 3190 . C 0 . DP=20;I16=11,8,0,0,675,24915,0,0,1109,65641,0,0,344,7440,0,0;QS=3,0;MQSB=0.902014;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,123:4:0 0,15,148:5:0 17 3191 . T 0 . DP=21;I16=11,9,0,0,773,30203,0,0,1169,69241,0,0,340,7340,0,0;QS=3,0;MQSB=0.916401;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,130:4:0 0,15,171:5:0 17 3192 . G 0 . DP=21;I16=12,9,0,0,751,27785,0,0,1198,70082,0,0,362,7886,0,0;QS=3,0;MQSB=0.994334;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,125:4:0 0,15,152:5:0 17 3193 . G 0 . DP=21;I16=12,9,0,0,756,27614,0,0,1198,70082,0,0,359,7829,0,0;QS=3,0;MQSB=0.994334;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,121:4:0 0,15,148:5:0 17 3194 . T C, 0 . DP=21;I16=10,10,1,0,730,26992,18,324,1169,69241,29,841,332,7168,25,625;QS=2.95652,0.0434783,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.99938;BQB=1;MQ0F=0 PL:DP:DV 0,18,255,33,255,255:12:1 0,9,98,9,98,98:3:0 0,18,178,18,178,178:6:0 17 3195 . T 0 . DP=21;I16=11,10,0,0,776,29184,0,0,1198,70082,0,0,356,7778,0,0;QS=3,0;MQSB=0.99938;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,95:3:0 0,18,189:6:0 17 3196 . T 0 . DP=21;I16=10,10,0,0,722,26472,0,0,1169,69241,0,0,329,7111,0,0;QS=3,0;MQSB=0.931063;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,91:3:0 0,18,186:6:0 17 3197 . A 0 . DP=22;I16=12,9,0,0,715,24911,0,0,1229,72841,0,0,352,7718,0,0;QS=3,0;MQSB=0.950149;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,103:3:0 0,18,179:6:0 17 3198 . A 0 . DP=21;I16=12,8,0,0,745,28155,0,0,1169,69241,0,0,345,7675,0,0;QS=3,0;MQSB=0.953497;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,105:3:0 0,18,198:6:0 17 3199 . A 0 . DP=21;I16=11,9,0,0,736,27514,0,0,1200,72000,0,0,326,7080,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,105:3:0 0,18,196:6:0 17 3200 . A 0 . DP=20;I16=11,9,0,0,735,27533,0,0,1169,69241,0,0,350,7660,0,0;QS=3,0;MQSB=0.943233;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,108:3:0 0,18,195:6:0 17 3201 . A 0 . DP=20;I16=11,8,0,0,706,26814,0,0,1109,65641,0,0,338,7486,0,0;QS=3,0;MQSB=0.946915;MQ0F=0 PL:DP:DV 0,30,248:10:0 0,9,107:3:0 0,18,198:6:0 17 3202 . T 0 . DP=20;I16=10,9,0,0,692,25620,0,0,1140,68400,0,0,321,6907,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,249:10:0 0,9,109:3:0 0,18,187:6:0 17 3203 . G A, 0 . DP=19;I16=9,9,1,0,644,23760,19,361,1080,64800,29,841,320,6872,25,625;QS=2.94823,0.0517711,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.934728;BQB=1;MQ0F=0 PL:DP:DV 0,14,239,30,242,248:11:1 0,9,105,9,105,105:3:0 0,15,160,15,160,160:5:0 17 3204 . T 0 . DP=19;I16=9,8,0,0,619,22955,0,0,1020,61200,0,0,306,6686,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,234:9:0 0,9,101:3:0 0,15,169:5:0 17 3205 . C 0 . DP=19;I16=9,9,0,0,672,25340,0,0,1080,64800,0,0,318,6856,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,99:3:0 0,15,157:5:0 17 3206 . A 0 . DP=19;I16=10,9,0,0,661,23623,0,0,1109,65641,0,0,342,7500,0,0;QS=3,0;MQSB=0.934728;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,91:3:0 0,15,165:5:0 17 3207 . C 0 . DP=19;I16=9,9,0,0,642,23618,0,0,1080,64800,0,0,316,6912,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,90:3:0 0,15,158:5:0 17 3208 . A 0 . DP=18;I16=10,8,0,0,619,22023,0,0,1049,62041,0,0,341,7591,0,0;QS=3,0;MQSB=0.938795;MQ0F=0 PL:DP:DV 0,33,247:11:0 0,9,95:3:0 0,12,142:4:0 17 3209 . C 0 . DP=19;I16=11,8,0,0,681,24747,0,0,1109,65641,0,0,340,7612,0,0;QS=3,0;MQSB=0.946915;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,106:3:0 0,12,134:4:0 17 3210 . C 0 . DP=18;I16=11,7,0,0,664,24900,0,0,1049,62041,0,0,340,7602,0,0;QS=3,0;MQSB=0.951002;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,101:3:0 0,12,141:4:0 17 3211 . A 0 . DP=17;I16=11,6,0,0,645,24627,0,0,989,58441,0,0,341,7611,0,0;QS=3,0;MQSB=0.955563;MQ0F=0 PL:DP:DV 0,30,243:10:0 0,9,106:3:0 0,12,140:4:0 17 3212 . T 0 . DP=17;I16=10,6,0,0,575,21295,0,0,960,57600,0,0,316,6964,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,222:9:0 0,9,100:3:0 0,12,144:4:0 17 3213 . A 0 . DP=17;I16=10,6,0,0,597,22631,0,0,960,57600,0,0,316,6962,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,228:9:0 0,9,117:3:0 0,12,138:4:0 17 3214 . G 0 . DP=17;I16=11,6,0,0,615,23103,0,0,989,58441,0,0,341,7605,0,0;QS=3,0;MQSB=0.955563;MQ0F=0 PL:DP:DV 0,30,243:10:0 0,9,110:3:0 0,12,134:4:0 17 3215 . G 0 . DP=17;I16=11,6,0,0,591,21523,0,0,989,58441,0,0,340,7592,0,0;QS=3,0;MQSB=0.955563;MQ0F=0 PL:DP:DV 0,30,241:10:0 0,9,93:3:0 0,12,128:4:0 17 3216 . C 0 . DP=17;I16=11,6,0,0,623,23303,0,0,989,58441,0,0,339,7597,0,0;QS=3,0;MQSB=0.955563;MQ0F=0 PL:DP:DV 0,30,242:10:0 0,9,94:3:0 0,12,138:4:0 17 3217 . C 0 . DP=17;I16=11,6,0,0,570,19896,0,0,989,58441,0,0,337,7569,0,0;QS=3,0;MQSB=0.955563;MQ0F=0 PL:DP:DV 0,30,236:10:0 0,9,84:3:0 0,12,123:4:0 17 3218 . G C, 0 . DP=18;I16=10,7,1,0,547,18185,29,841,1020,61200,29,841,310,6932,24,576;QS=2.91667,0.0833333,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.951002;BQB=1;MQ0F=0 PL:DP:DV 0,4,202,30,205,221:11:1 0,9,96,9,96,96:3:0 0,12,112,12,112,112:4:0 17 3219 . G A, 0 . DP=18;I16=10,7,1,0,606,22158,19,361,1020,61200,29,841,308,6888,23,529;QS=2.94837,0.0516304,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.951002;BQB=1;MQ0F=0 PL:DP:DV 0,14,234,30,237,243:11:1 0,9,107,9,107,107:3:0 0,12,126,12,126,126:4:0 17 3220 . G 0 . DP=18;I16=11,7,0,0,631,22827,0,0,1049,62041,0,0,326,7248,0,0;QS=3,0;MQSB=0.951002;MQ0F=0 PL:DP:DV 0,33,252:11:0 0,9,112:3:0 0,12,126:4:0 17 3221 . C 0 . DP=17;I16=9,7,0,0,598,22586,0,0,960,57600,0,0,301,6659,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,244:9:0 0,9,103:3:0 0,12,139:4:0 17 3222 . A 0 . DP=17;I16=10,7,0,0,623,23037,0,0,989,58441,0,0,318,6972,0,0;QS=3,0;MQSB=0.943335;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,100:3:0 0,12,133:4:0 17 3223 . C 0 . DP=17;I16=10,7,0,0,611,22603,0,0,989,58441,0,0,312,6764,0,0;QS=3,0;MQSB=0.943335;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,96:3:0 0,12,132:4:0 17 3224 . A 0 . DP=16;I16=10,6,0,0,609,23381,0,0,929,54841,0,0,307,6575,0,0;QS=3,0;MQSB=0.948436;MQ0F=0 PL:DP:DV 0,27,238:9:0 0,9,97:3:0 0,12,141:4:0 17 3225 . G 0 . DP=17;I16=11,6,0,0,604,22692,0,0,989,58441,0,0,302,6404,0,0;QS=3,0;MQSB=0.955563;MQ0F=0 PL:DP:DV 0,27,247:9:0 0,12,108:4:0 0,12,134:4:0 17 3226 . T 0 . DP=18;I16=11,6,0,0,582,20466,0,0,1020,61200,0,0,282,5996,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,224:9:0 0,12,112:4:0 0,12,131:4:0 17 3227 . G 0 . DP=18;I16=12,5,0,0,618,23008,0,0,989,58441,0,0,270,5496,0,0;QS=3,0;MQSB=0.965874;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,91:3:0 0,12,125:4:0 17 3228 . G 0 . DP=18;I16=11,6,0,0,611,22581,0,0,1020,61200,0,0,278,5816,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,248:9:0 0,12,118:4:0 0,12,121:4:0 17 3229 . C 0 . DP=19;I16=12,7,0,0,686,25394,0,0,1109,65641,0,0,289,5925,0,0;QS=3,0;MQSB=0.957193;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,118:4:0 0,12,126:4:0 17 3230 . T 0 . DP=19;I16=12,6,0,0,669,25441,0,0,1049,62041,0,0,261,5187,0,0;QS=3,0;MQSB=0.961295;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,107:3:0 0,12,139:4:0 17 3231 . C 0 . DP=19;I16=12,7,0,0,682,25046,0,0,1109,65641,0,0,283,5725,0,0;QS=3,0;MQSB=0.957193;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,112:4:0 0,12,128:4:0 17 3232 . A 0 . DP=19;I16=11,7,0,0,597,20779,0,0,1080,64800,0,0,270,5564,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,229:10:0 0,12,117:4:0 0,12,132:4:0 17 3233 . C 0 . DP=19;I16=12,7,0,0,573,18239,0,0,1109,65641,0,0,277,5629,0,0;QS=3,0;MQSB=0.957193;MQ0F=0 PL:DP:DV 0,33,238:11:0 0,12,91:4:0 0,12,110:4:0 17 3234 . G T, 0 . DP=19;I16=10,6,1,0,511,17305,22,484,960,57600,29,841,233,4849,8,64;QS=2.93855,0.0614525,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.955563;BQB=1;MQ0F=0 PL:DP:DV 0,11,222,30,225,234:11:1 0,6,63,6,63,63:2:0 0,12,100,12,100,100:4:0 17 3235 . C 0 . DP=18;I16=12,6,0,0,625,22649,0,0,1049,62041,0,0,274,5582,0,0;QS=3,0;MQSB=0.961295;MQ0F=0 PL:DP:DV 0,30,249:10:0 0,12,119:4:0 0,12,123:4:0 17 3236 . C 0 . DP=18;I16=12,6,0,0,668,25124,0,0,1049,62041,0,0,273,5567,0,0;QS=3,0;MQSB=0.961295;MQ0F=0 PL:DP:DV 0,30,244:10:0 0,12,137:4:0 0,12,134:4:0 17 3237 . T 0 . DP=17;I16=11,6,0,0,589,21235,0,0,989,58441,0,0,273,5573,0,0;QS=3,0;MQSB=0.955563;MQ0F=0 PL:DP:DV 0,27,230:9:0 0,12,102:4:0 0,12,136:4:0 17 3238 . G 0 . DP=17;I16=11,6,0,0,609,22539,0,0,989,58441,0,0,273,5599,0,0;QS=3,0;MQSB=0.955563;MQ0F=0 PL:DP:DV 0,27,243:9:0 0,12,104:4:0 0,12,129:4:0 17 3239 . T 0 . DP=17;I16=11,6,0,0,574,20204,0,0,989,58441,0,0,273,5645,0,0;QS=3,0;MQSB=0.955563;MQ0F=0 PL:DP:DV 0,27,220:9:0 0,12,107:4:0 0,12,131:4:0 17 3240 . A 0 . DP=19;I16=12,7,0,0,624,21552,0,0,1109,65641,0,0,273,5711,0,0;QS=3,0;MQSB=0.957193;MQ0F=0 PL:DP:DV 0,33,248:11:0 0,12,101:4:0 0,12,132:4:0 17 3241 . A 0 . DP=19;I16=11,6,0,0,619,23121,0,0,1020,61200,0,0,249,5173,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,252:10:0 0,9,96:3:0 0,12,143:4:0 17 3242 . T 0 . DP=19;I16=12,7,0,0,655,23279,0,0,1140,68400,0,0,277,5911,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,252:10:0 0,12,104:4:0 0,15,152:5:0 17 3243 . C 0 . DP=19;I16=12,7,0,0,682,25216,0,0,1140,68400,0,0,281,6047,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,111:4:0 0,15,148:5:0 17 3244 . C 0 . DP=18;I16=11,6,0,0,587,21119,0,0,1020,61200,0,0,281,6139,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,221:8:0 0,12,116:4:0 0,15,142:5:0 17 3245 . C 0 . DP=17;I16=10,7,0,0,631,23851,0,0,1020,61200,0,0,290,6282,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,12,126:4:0 0,12,131:4:0 17 3246 . A 0 . DP=17;I16=10,7,0,0,646,24716,0,0,1020,61200,0,0,295,6427,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,12,129:4:0 0,12,139:4:0 17 3247 . G 0 . DP=17;I16=10,7,0,0,606,22554,0,0,1020,61200,0,0,300,6590,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,12,92:4:0 0,12,133:4:0 17 3248 . C 0 . DP=16;I16=10,6,0,0,603,23115,0,0,960,57600,0,0,306,6770,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,9,99:3:0 0,12,132:4:0 17 3249 . C 0 . DP=16;I16=10,6,0,0,587,21913,0,0,960,57600,0,0,311,6917,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,246:9:0 0,9,99:3:0 0,12,133:4:0 17 3250 . C 0 . DP=16;I16=10,6,0,0,616,24080,0,0,960,57600,0,0,316,7082,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,9,95:3:0 0,12,137:4:0 17 3251 . T 0 . DP=16;I16=10,6,0,0,585,22193,0,0,960,57600,0,0,319,7165,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,241:9:0 0,9,104:3:0 0,12,135:4:0 17 3252 . T 0 . DP=16;I16=10,6,0,0,553,19781,0,0,960,57600,0,0,321,7215,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,239:9:0 0,9,84:3:0 0,12,126:4:0 17 3253 . T 0 . DP=16;I16=10,6,0,0,589,21933,0,0,960,57600,0,0,323,7281,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,245:9:0 0,9,102:3:0 0,12,132:4:0 17 3254 . G 0 . DP=17;I16=10,7,0,0,631,23737,0,0,1020,61200,0,0,325,7363,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,9,100:3:0 0,15,154:5:0 17 3255 . G 0 . DP=16;I16=9,7,0,0,554,20088,0,0,960,57600,0,0,328,7410,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,243:9:0 0,9,93:3:0 0,12,120:4:0 17 3256 . G 0 . DP=16;I16=8,7,0,0,571,21985,0,0,900,54000,0,0,306,6846,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,255:9:0 0,6,62:2:0 0,12,142:4:0 17 3257 . A 0 . DP=16;I16=9,7,0,0,585,21881,0,0,960,57600,0,0,334,7546,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,243:9:0 0,9,94:3:0 0,12,144:4:0 17 3258 . G 0 . DP=17;I16=9,8,0,0,647,25407,0,0,1020,61200,0,0,337,7635,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,81:3:0 0,12,146:4:0 17 3259 . G 0 . DP=17;I16=9,8,0,0,605,22237,0,0,1020,61200,0,0,341,7739,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,9,79:3:0 0,12,139:4:0 17 3260 . C 0 . DP=17;I16=9,7,0,0,616,23908,0,0,960,57600,0,0,319,7183,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,6,64:2:0 0,12,133:4:0 17 3261 . C 0 . DP=18;I16=9,9,0,0,655,24475,0,0,1080,64800,0,0,347,7891,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,90:3:0 0,12,138:4:0 17 3262 . A 0 . DP=19;I16=10,9,0,0,712,27036,0,0,1140,68400,0,0,351,7989,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,123:4:0 0,12,148:4:0 17 3263 . G 0 . DP=19;I16=10,9,0,0,715,27317,0,0,1140,68400,0,0,356,8104,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,116:4:0 0,12,146:4:0 17 3264 . G 0 . DP=19;I16=10,9,0,0,676,24734,0,0,1140,68400,0,0,361,8237,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,93:4:0 0,12,143:4:0 17 3265 . G 0 . DP=19;I16=10,9,0,0,701,26199,0,0,1140,68400,0,0,365,8339,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,109:4:0 0,12,137:4:0 17 3266 . T 0 . DP=19;I16=9,9,0,0,597,20769,0,0,1080,64800,0,0,357,8229,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,82:4:0 0,9,113:3:0 17 3267 . G 0 . DP=19;I16=10,9,0,0,659,24285,0,0,1140,68400,0,0,367,8299,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,76:4:0 0,12,133:4:0 17 3268 . G 0 . DP=19;I16=10,9,0,0,668,24306,0,0,1140,68400,0,0,367,8255,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,92:4:0 0,12,142:4:0 17 3269 . G 0 . DP=19;I16=10,9,0,0,714,27122,0,0,1140,68400,0,0,367,8227,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,115:4:0 0,12,137:4:0 17 3270 . T 0 . DP=20;I16=10,9,0,0,606,20364,0,0,1140,68400,0,0,361,8141,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,251:11:0 0,12,83:4:0 0,12,136:4:0 17 3271 . G 0 . DP=19;I16=10,9,0,0,709,26993,0,0,1140,68400,0,0,362,8108,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,100:4:0 0,12,143:4:0 17 3272 . G 0 . DP=19;I16=10,9,0,0,672,24398,0,0,1140,68400,0,0,363,8093,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,97:4:0 0,12,140:4:0 17 3273 . A 0 . DP=20;I16=10,10,0,0,735,27355,0,0,1200,72000,0,0,363,8047,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,153:5:0 0,12,147:4:0 17 3274 . T 0 . DP=19;I16=9,10,0,0,699,26089,0,0,1140,68400,0,0,365,8021,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,142:5:0 0,12,149:4:0 17 3275 . C 0 . DP=19;I16=9,10,0,0,727,28197,0,0,1140,68400,0,0,367,8015,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,137:5:0 0,12,139:4:0 17 3276 . A 0 . DP=19;I16=9,10,0,0,681,25123,0,0,1140,68400,0,0,369,8029,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,253:10:0 0,15,150:5:0 0,12,141:4:0 17 3277 . C 0 . DP=19;I16=9,10,0,0,733,28485,0,0,1140,68400,0,0,371,8063,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,153:5:0 0,12,146:4:0 17 3278 . T 0 . DP=19;I16=9,10,0,0,737,28901,0,0,1140,68400,0,0,373,8117,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,168:5:0 0,12,153:4:0 17 3279 . T 0 . DP=19;I16=9,10,0,0,714,27132,0,0,1140,68400,0,0,375,8191,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,148:5:0 0,12,150:4:0 17 3280 . G 0 . DP=20;I16=9,11,0,0,758,29178,0,0,1200,72000,0,0,376,8234,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,143:5:0 0,12,146:4:0 17 3281 . A 0 . DP=21;I16=10,11,0,0,822,32654,0,0,1260,75600,0,0,378,8296,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,179:6:0 0,12,157:4:0 17 3282 . G 0 . DP=21;I16=10,11,0,0,815,32059,0,0,1260,75600,0,0,381,8379,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,173:6:0 0,12,150:4:0 17 3283 . G 0 . DP=21;I16=10,11,0,0,790,30110,0,0,1260,75600,0,0,384,8484,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,161:6:0 0,12,146:4:0 17 3284 . T 0 . DP=21;I16=10,11,0,0,748,27628,0,0,1260,75600,0,0,385,8511,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,155:6:0 0,12,146:4:0 17 3285 . C 0 . DP=21;I16=10,11,0,0,776,29442,0,0,1260,75600,0,0,386,8560,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,169:6:0 0,12,143:4:0 17 3286 . A 0 . DP=21;I16=10,11,0,0,826,32732,0,0,1260,75600,0,0,387,8631,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,187:6:0 0,12,153:4:0 17 3287 . G 0 . DP=21;I16=10,11,0,0,804,31448,0,0,1260,75600,0,0,387,8673,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,164:6:0 0,12,150:4:0 17 3288 . G 0 . DP=22;I16=10,11,0,0,754,27862,0,0,1260,75600,0,0,379,8635,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,141:5:0 0,15,164:5:0 17 3289 . A 0 . DP=24;I16=13,11,0,0,897,34439,0,0,1440,86400,0,0,386,8714,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,166:6:0 0,15,178:5:0 17 3290 . G 0 . DP=23;I16=12,10,0,0,832,31964,0,0,1320,79200,0,0,380,8684,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,112:4:0 0,15,168:5:0 17 3291 . T 0 . DP=22;I16=11,9,0,0,707,25481,0,0,1200,72000,0,0,358,8112,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,9,95:3:0 0,15,168:5:0 17 3292 . T 0 . DP=22;I16=13,9,0,0,783,28439,0,0,1320,79200,0,0,397,8929,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,127:5:0 0,15,164:5:0 17 3293 . C 0 . DP=22;I16=13,9,0,0,852,33430,0,0,1320,79200,0,0,400,8992,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,136:5:0 0,15,170:5:0 17 3294 . A 0 . DP=22;I16=13,9,0,0,818,30684,0,0,1320,79200,0,0,403,9077,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,145:5:0 0,15,162:5:0 17 3295 . A 0 . DP=22;I16=14,8,0,0,820,31004,0,0,1320,79200,0,0,407,9183,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,140:5:0 0,12,143:4:0 17 3296 . G 0 . DP=22;I16=14,8,0,0,837,32209,0,0,1320,79200,0,0,411,9259,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,133:5:0 0,12,140:4:0 17 3297 . A 0 . DP=22;I16=14,8,0,0,787,28771,0,0,1320,79200,0,0,415,9355,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,134:5:0 0,12,140:4:0 17 3298 . C 0 . DP=22;I16=13,9,0,0,826,31506,0,0,1320,79200,0,0,420,9470,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,113:4:0 0,12,135:4:0 17 3299 . C 0 . DP=22;I16=13,9,0,0,857,33829,0,0,1320,79200,0,0,425,9553,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,117:4:0 0,12,140:4:0 17 3300 . A 0 . DP=22;I16=13,9,0,0,861,33855,0,0,1320,79200,0,0,430,9654,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,134:4:0 0,12,144:4:0 17 3301 . G 0 . DP=22;I16=13,9,0,0,869,34495,0,0,1320,79200,0,0,433,9675,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,131:4:0 0,12,141:4:0 17 3302 . C 0 . DP=23;I16=14,9,0,0,863,32885,0,0,1380,82800,0,0,436,9718,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,115:4:0 0,15,155:5:0 17 3303 . C 0 . DP=23;I16=14,9,0,0,887,34451,0,0,1380,82800,0,0,440,9784,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,125:4:0 0,15,153:5:0 17 3304 . T 0 . DP=23;I16=14,9,0,0,886,34388,0,0,1380,82800,0,0,443,9825,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,134:4:0 0,15,167:5:0 17 3305 . G 0 . DP=23;I16=14,9,0,0,878,33846,0,0,1380,82800,0,0,446,9892,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,119:4:0 0,15,151:5:0 17 3306 . G 0 . DP=23;I16=14,9,0,0,886,34386,0,0,1380,82800,0,0,448,9934,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,127:4:0 0,15,150:5:0 17 3307 . C 0 . DP=23;I16=14,8,0,0,812,30814,0,0,1320,79200,0,0,428,9508,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,116:4:0 0,15,153:5:0 17 3308 . C 0 . DP=23;I16=14,9,0,0,835,31367,0,0,1380,82800,0,0,450,9986,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,118:4:0 0,15,151:5:0 17 3309 . A 0 . DP=23;I16=14,9,0,0,875,33541,0,0,1380,82800,0,0,451,9995,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,128:4:0 0,15,162:5:0 17 3310 . A 0 . DP=24;I16=15,9,0,0,882,32950,0,0,1440,86400,0,0,453,10027,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,152:5:0 0,15,164:5:0 17 3311 . C 0 . DP=24;I16=15,9,0,0,913,35161,0,0,1440,86400,0,0,456,10084,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,145:5:0 0,15,157:5:0 17 3312 . A 0 . DP=26;I16=16,9,0,0,950,36336,0,0,1500,90000,0,0,459,10167,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,153:5:0 0,15,160:5:0 17 3313 . T 0 . DP=26;I16=16,10,0,0,987,37617,0,0,1560,93600,0,0,488,10902,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,180:6:0 0,15,161:5:0 17 3314 . G 0 . DP=26;I16=16,10,0,0,1007,39401,0,0,1560,93600,0,0,491,10989,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,171:6:0 0,15,161:5:0 17 3315 . G 0 . DP=26;I16=15,10,0,0,945,36073,0,0,1500,90000,0,0,484,10866,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,172:6:0 0,15,159:5:0 17 3316 . T 0 . DP=26;I16=15,10,0,0,853,29881,0,0,1500,90000,0,0,464,10216,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,157:6:0 0,12,122:4:0 17 3317 . G 0 . DP=27;I16=16,11,0,0,997,37431,0,0,1620,97200,0,0,488,10806,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,164:6:0 0,18,167:6:0 17 3318 . A 0 . DP=26;I16=15,10,0,0,904,33212,0,0,1500,90000,0,0,481,10699,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,155:5:0 0,18,185:6:0 17 3319 . A 0 . DP=25;I16=15,10,0,0,912,34090,0,0,1500,90000,0,0,482,10682,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,139:5:0 0,18,188:6:0 17 3320 . A 0 . DP=25;I16=15,10,0,0,888,32722,0,0,1500,90000,0,0,483,10691,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,133:5:0 0,18,178:6:0 17 3321 . C 0 . DP=25;I16=14,10,0,0,900,34142,0,0,1440,86400,0,0,471,10531,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,157:5:0 0,18,186:6:0 17 3322 . C 0 . DP=25;I16=15,10,0,0,980,38582,0,0,1500,90000,0,0,483,10683,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,164:5:0 0,18,198:6:0 17 3323 . C 0 . DP=25;I16=15,10,0,0,922,34948,0,0,1500,90000,0,0,483,10715,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,146:5:0 0,18,201:6:0 17 3324 . C 0 . DP=25;I16=15,10,0,0,869,31271,0,0,1500,90000,0,0,482,10720,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,142:5:0 0,18,186:6:0 17 3325 . G 0 . DP=25;I16=15,10,0,0,827,28293,0,0,1500,90000,0,0,481,10747,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,134:5:0 0,18,163:6:0 17 3326 . T 0 . DP=24;I16=13,10,0,0,842,31362,0,0,1380,82800,0,0,464,10506,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,150:5:0 0,18,186:6:0 17 3327 . C 0 . DP=24;I16=14,10,0,0,938,37076,0,0,1440,86400,0,0,481,10863,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,154:5:0 0,18,190:6:0 17 3328 . T 0 . DP=25;I16=15,10,0,0,959,37033,0,0,1500,90000,0,0,480,10900,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,163:5:0 0,21,213:7:0 17 3329 . A 0 . DP=24;I16=15,9,0,0,888,33082,0,0,1440,86400,0,0,481,10955,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,149:5:0 0,21,207:7:0 17 3330 . C 0 . DP=25;I16=16,9,0,0,954,37064,0,0,1500,90000,0,0,481,10979,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,168:5:0 0,21,203:7:0 17 3331 . T 0 . DP=25;I16=16,9,0,0,960,37332,0,0,1500,90000,0,0,482,11024,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,165:5:0 0,21,224:7:0 17 3332 . A 0 . DP=25;I16=16,9,0,0,933,35065,0,0,1500,90000,0,0,483,11091,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,165:5:0 0,21,211:7:0 17 3333 . A 0 . DP=26;I16=16,10,0,0,976,37344,0,0,1560,93600,0,0,483,11131,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,166:5:0 0,24,241:8:0 17 3334 . A 0 . DP=25;I16=15,10,0,0,972,38210,0,0,1500,90000,0,0,485,11195,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,168:5:0 0,24,241:8:0 17 3335 . A 0 . DP=25;I16=15,10,0,0,970,38200,0,0,1500,90000,0,0,486,11232,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,170:5:0 0,24,239:8:0 17 3336 . A 0 . DP=25;I16=15,10,0,0,929,35277,0,0,1500,90000,0,0,485,11191,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,166:5:0 0,24,232:8:0 17 3337 . T 0 . DP=25;I16=15,10,0,0,902,32856,0,0,1500,90000,0,0,484,11172,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,157:5:0 0,24,224:8:0 17 3338 . A 0 . DP=25;I16=15,10,0,0,892,32056,0,0,1500,90000,0,0,481,11075,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,153:5:0 0,24,214:8:0 17 3339 . C 0 . DP=25;I16=15,10,0,0,964,37444,0,0,1500,90000,0,0,478,11000,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,167:5:0 0,24,232:8:0 17 3340 . A 0 . DP=23;I16=14,9,0,0,890,34616,0,0,1380,82800,0,0,477,10945,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,173:5:0 0,24,233:8:0 17 3341 . A 0 . DP=23;I16=14,9,0,0,885,34459,0,0,1380,82800,0,0,476,10908,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,167:5:0 0,24,238:8:0 17 3342 . A 0 . DP=23;I16=14,9,0,0,862,33262,0,0,1380,82800,0,0,475,10889,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,170:5:0 0,24,240:8:0 17 3343 . A 0 . DP=22;I16=13,9,0,0,866,34280,0,0,1320,79200,0,0,474,10836,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,173:5:0 0,21,231:7:0 17 3344 . A 0 . DP=22;I16=13,9,0,0,859,33731,0,0,1320,79200,0,0,473,10797,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,173:5:0 0,21,226:7:0 17 3345 . T 0 . DP=22;I16=13,9,0,0,822,31100,0,0,1320,79200,0,0,472,10772,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,254:10:0 0,15,159:5:0 0,21,220:7:0 17 3346 . T 0 . DP=22;I16=13,9,0,0,847,32679,0,0,1320,79200,0,0,470,10712,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,168:5:0 0,21,221:7:0 17 3347 . A 0 . DP=23;I16=14,9,0,0,888,34650,0,0,1380,82800,0,0,468,10668,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,185:6:0 0,21,227:7:0 17 3348 . G 0 . DP=23;I16=14,9,0,0,873,33609,0,0,1380,82800,0,0,467,10641,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,171:6:0 0,21,219:7:0 17 3349 . C 0 . DP=24;I16=14,9,0,0,815,29785,0,0,1380,82800,0,0,465,10583,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,166:6:0 0,21,205:7:0 17 3350 . C 0 . DP=24;I16=14,10,0,0,939,37249,0,0,1440,86400,0,0,464,10546,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,209:7:0 0,21,220:7:0 17 3351 . T 0 . DP=24;I16=14,10,0,0,943,37255,0,0,1440,86400,0,0,463,10531,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,210:7:0 0,21,228:7:0 17 3352 . G 0 . DP=24;I16=14,10,0,0,887,33267,0,0,1440,86400,0,0,462,10538,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,192:7:0 0,21,214:7:0 17 3353 . G 0 . DP=24;I16=14,10,0,0,864,32092,0,0,1440,86400,0,0,461,10567,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,190:7:0 0,21,214:7:0 17 3354 . C 0 . DP=24;I16=14,10,0,0,848,30714,0,0,1440,86400,0,0,459,10567,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,178:7:0 0,21,209:7:0 17 3355 . G 0 . DP=23;I16=14,9,0,0,738,24386,0,0,1380,82800,0,0,457,10537,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,232:10:0 0,21,170:7:0 0,18,166:6:0 17 3356 . T 0 . DP=23;I16=14,9,0,0,847,31689,0,0,1380,82800,0,0,454,10476,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,191:7:0 0,18,195:6:0 17 3357 . G 0 . DP=23;I16=14,9,0,0,863,32919,0,0,1380,82800,0,0,449,10335,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,193:7:0 0,18,192:6:0 17 3358 . G 0 . DP=22;I16=14,8,0,0,810,30184,0,0,1320,79200,0,0,445,10215,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,239:9:0 0,21,200:7:0 0,18,187:6:0 17 3359 . T 0 . DP=23;I16=13,8,0,0,743,26737,0,0,1260,75600,0,0,404,9318,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,228:9:0 0,21,194:7:0 0,15,162:5:0 17 3360 . G 0 . DP=23;I16=15,8,0,0,822,30058,0,0,1380,82800,0,0,436,9932,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,186:7:0 0,18,175:6:0 17 3361 . G 0 . DP=22;I16=15,7,0,0,751,26793,0,0,1320,79200,0,0,433,9819,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,218:9:0 0,21,187:7:0 0,18,175:6:0 17 3362 . C 0 . DP=22;I16=15,7,0,0,768,27462,0,0,1320,79200,0,0,430,9724,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,230:9:0 0,21,178:7:0 0,18,173:6:0 17 3363 . G 0 . DP=21;I16=14,7,0,0,662,21572,0,0,1260,75600,0,0,428,9646,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,194:9:0 0,18,155:6:0 0,18,161:6:0 17 3364 . C 0 . DP=21;I16=14,7,0,0,818,32184,0,0,1260,75600,0,0,423,9437,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,241:9:0 0,18,191:6:0 0,18,194:6:0 17 3365 . A 0 . DP=21;I16=14,7,0,0,791,29983,0,0,1260,75600,0,0,418,9250,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,226:9:0 0,18,186:6:0 0,18,190:6:0 17 3366 . T 0 . DP=21;I16=14,7,0,0,759,28053,0,0,1260,75600,0,0,413,9085,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,221:9:0 0,18,185:6:0 0,18,177:6:0 17 3367 . G 0 . DP=21;I16=14,7,0,0,767,28539,0,0,1260,75600,0,0,408,8942,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,235:9:0 0,18,185:6:0 0,18,169:6:0 17 3368 . C 0 . DP=21;I16=14,7,0,0,807,31329,0,0,1260,75600,0,0,403,8821,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,245:9:0 0,18,181:6:0 0,18,189:6:0 17 3369 . C 0 . DP=21;I16=14,7,0,0,789,30271,0,0,1260,75600,0,0,398,8722,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,228:9:0 0,18,186:6:0 0,18,193:6:0 17 3370 . T 0 . DP=21;I16=13,7,0,0,798,31960,0,0,1200,72000,0,0,392,8596,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,239:9:0 0,18,201:6:0 0,15,178:5:0 17 3371 . G 0 . DP=20;I16=13,7,0,0,739,27875,0,0,1200,72000,0,0,387,8493,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,233:9:0 0,18,173:6:0 0,15,169:5:0 17 3372 . T 0 . DP=20;I16=13,6,0,0,693,25677,0,0,1140,68400,0,0,359,7883,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,220:9:0 0,15,154:5:0 0,15,167:5:0 17 3373 . A 0 . DP=20;I16=13,7,0,0,729,27019,0,0,1200,72000,0,0,375,8253,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,226:9:0 0,18,162:6:0 0,15,174:5:0 17 3374 . A 0 . DP=19;I16=13,6,0,0,719,27437,0,0,1140,68400,0,0,369,8115,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,225:9:0 0,15,167:5:0 0,15,164:5:0 17 3375 . T 0 . DP=20;I16=12,6,0,0,683,26039,0,0,1080,64800,0,0,337,7321,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,221:8:0 0,15,155:5:0 0,15,172:5:0 17 3376 . C 0 . DP=20;I16=13,7,0,0,739,27867,0,0,1200,72000,0,0,356,7796,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,254:10:0 0,15,146:5:0 0,15,173:5:0 17 3377 . C 0 . DP=20;I16=13,7,0,0,741,27905,0,0,1200,72000,0,0,350,7666,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,252:10:0 0,15,149:5:0 0,15,173:5:0 17 3378 . C 0 . DP=20;I16=13,7,0,0,775,30179,0,0,1200,72000,0,0,343,7507,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,15,161:5:0 0,15,172:5:0 17 3379 . A 0 . DP=20;I16=13,7,0,0,736,27530,0,0,1200,72000,0,0,336,7370,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,245:10:0 0,15,157:5:0 0,15,169:5:0 17 3380 . G 0 . DP=19;I16=13,6,0,0,729,28249,0,0,1140,68400,0,0,330,7254,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,233:9:0 0,15,157:5:0 0,15,178:5:0 17 3381 . C 0 . DP=19;I16=13,6,0,0,742,29288,0,0,1140,68400,0,0,324,7158,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,241:9:0 0,15,155:5:0 0,15,178:5:0 17 3382 . T 0 . DP=17;I16=12,5,0,0,649,25245,0,0,1020,61200,0,0,320,7080,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,222:9:0 0,9,105:3:0 0,15,184:5:0 17 3383 . A 0 . DP=17;I16=12,4,0,0,556,19808,0,0,960,57600,0,0,291,6393,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,27,196:9:0 0,6,71:2:0 0,15,167:5:0 17 3384 . C 0 . DP=19;I16=14,5,0,0,701,26181,0,0,1140,68400,0,0,311,6923,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,235:10:0 0,9,98:3:0 0,18,187:6:0 17 3385 . T 0 . DP=19;I16=14,5,0,0,730,28456,0,0,1140,68400,0,0,307,6797,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,237:10:0 0,9,109:3:0 0,18,195:6:0 17 3386 . T 0 . DP=19;I16=14,5,0,0,642,22450,0,0,1140,68400,0,0,302,6642,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,197:10:0 0,9,104:3:0 0,18,188:6:0 17 3387 . G 0 . DP=20;I16=14,6,0,0,723,26761,0,0,1200,72000,0,0,296,6460,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,230:10:0 0,9,105:3:0 0,21,210:7:0 17 3388 . G 0 . DP=20;I16=14,6,0,0,705,25891,0,0,1200,72000,0,0,291,6303,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,225:10:0 0,9,109:3:0 0,21,202:7:0 17 3389 . G 0 . DP=18;I16=10,7,0,0,617,22635,0,0,1020,61200,0,0,270,5808,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,190:7:0 0,9,105:3:0 0,21,207:7:0 17 3390 . A 0 . DP=18;I16=11,7,0,0,631,22681,0,0,1080,64800,0,0,288,6056,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,200:8:0 0,9,105:3:0 0,21,198:7:0 17 3391 . A 0 . DP=18;I16=11,7,0,0,663,24951,0,0,1080,64800,0,0,287,5965,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,192:8:0 0,9,113:3:0 0,21,224:7:0 17 3392 . G 0 . DP=18;I16=11,7,0,0,666,25104,0,0,1080,64800,0,0,286,5896,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,211:8:0 0,9,103:3:0 0,21,216:7:0 17 3393 . C 0 . DP=18;I16=11,7,0,0,686,26472,0,0,1080,64800,0,0,284,5800,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,222:8:0 0,9,106:3:0 0,21,214:7:0 17 3394 . T 0 . DP=18;I16=11,7,0,0,699,27481,0,0,1080,64800,0,0,282,5728,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,219:8:0 0,9,114:3:0 0,21,222:7:0 17 3395 . G 0 . DP=17;I16=10,7,0,0,656,25512,0,0,1020,61200,0,0,281,5679,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,205:7:0 0,9,105:3:0 0,21,222:7:0 17 3396 . A 0 . DP=17;I16=10,7,0,0,644,25446,0,0,1020,61200,0,0,280,5652,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,182:7:0 0,9,119:3:0 0,21,232:7:0 17 3397 . G 0 . DP=17;I16=10,7,0,0,633,23895,0,0,1020,61200,0,0,279,5647,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,193:7:0 0,9,107:3:0 0,21,218:7:0 17 3398 . G 0 . DP=16;I16=10,6,0,0,610,23380,0,0,960,57600,0,0,279,5663,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,170:6:0 0,9,110:3:0 0,21,215:7:0 17 3399 . G 0 . DP=17;I16=10,6,0,0,585,21729,0,0,960,57600,0,0,270,5618,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,15,147:5:0 0,12,122:4:0 0,21,216:7:0 17 3400 . A 0 . DP=17;I16=10,6,0,0,576,21400,0,0,960,57600,0,0,264,5500,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,15,136:5:0 0,12,131:4:0 0,21,213:7:0 17 3401 . T 0 . DP=17;I16=11,6,0,0,605,22159,0,0,1020,61200,0,0,280,5784,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,146:6:0 0,12,131:4:0 0,21,216:7:0 17 3402 . G 0 . DP=17;I16=11,6,0,0,638,24150,0,0,1020,61200,0,0,280,5832,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,172:6:0 0,12,130:4:0 0,21,213:7:0 17 3403 . A 0 . DP=16;I16=9,6,0,0,579,22815,0,0,900,54000,0,0,276,5874,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,15,139:5:0 0,12,140:4:0 0,18,205:6:0 17 3404 . G 0 . DP=16;I16=10,6,0,0,564,20652,0,0,960,57600,0,0,281,5935,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,148:6:0 0,12,131:4:0 0,18,186:6:0 17 3405 . A 0 . DP=16;I16=10,6,0,0,560,20158,0,0,960,57600,0,0,281,5991,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,157:6:0 0,12,124:4:0 0,18,179:6:0 17 3406 . A 0 . DP=16;I16=10,6,0,0,568,20556,0,0,960,57600,0,0,281,6067,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,150:6:0 0,12,129:4:0 0,18,186:6:0 17 3407 . C 0 . DP=17;I16=11,6,0,0,591,21225,0,0,1020,61200,0,0,281,6163,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,178:7:0 0,12,106:4:0 0,18,183:6:0 17 3408 . T 0 . DP=17;I16=11,6,0,0,632,23754,0,0,1020,61200,0,0,282,6280,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,21,177:7:0 0,12,133:4:0 0,18,189:6:0 17 3409 . G 0 . DP=16;I16=10,6,0,0,599,22667,0,0,960,57600,0,0,283,6369,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,172:6:0 0,12,119:4:0 0,18,192:6:0 17 3410 . C 0 . DP=16;I16=10,6,0,0,585,21685,0,0,960,57600,0,0,282,6378,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,174:6:0 0,12,126:4:0 0,18,176:6:0 17 3411 . T 0 . DP=15;I16=9,6,0,0,578,22492,0,0,900,54000,0,0,282,6404,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,165:6:0 0,9,114:3:0 0,18,196:6:0 17 3412 . T 0 . DP=15;I16=9,6,0,0,546,20202,0,0,900,54000,0,0,283,6445,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,15,148:5:0 0,12,120:4:0 0,18,189:6:0 17 3413 . G 0 . DP=15;I16=9,6,0,0,559,21023,0,0,900,54000,0,0,283,6401,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,15,158:5:0 0,12,124:4:0 0,18,184:6:0 17 3414 . A 0 . DP=15;I16=9,6,0,0,512,17878,0,0,900,54000,0,0,283,6373,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,15,148:5:0 0,12,116:4:0 0,18,166:6:0 17 3415 . A 0 . DP=16;I16=9,7,0,0,544,19656,0,0,960,57600,0,0,282,6310,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,15,142:5:0 0,12,132:4:0 0,21,179:7:0 17 3416 . C 0 . DP=16;I16=9,7,0,0,562,20168,0,0,960,57600,0,0,282,6262,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,15,143:5:0 0,12,127:4:0 0,21,191:7:0 17 3417 . C 0 . DP=16;I16=9,7,0,0,566,20666,0,0,960,57600,0,0,282,6230,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,15,147:5:0 0,12,126:4:0 0,21,192:7:0 17 3418 . T 0 . DP=17;I16=10,5,0,0,557,21637,0,0,900,54000,0,0,268,5988,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,15,150:5:0 0,15,148:5:0 0,15,176:5:0 17 3419 . G 0 . DP=17;I16=10,7,0,0,594,21642,0,0,1020,61200,0,0,310,6836,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,161:6:0 0,15,140:5:0 0,18,190:6:0 17 3420 . G 0 . DP=17;I16=10,7,0,0,596,21580,0,0,1020,61200,0,0,312,6850,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,172:6:0 0,15,132:5:0 0,18,188:6:0 17 3421 . G 0 . DP=18;I16=10,8,0,0,633,22781,0,0,1049,62041,0,0,314,6880,0,0;QS=3,0;MQSB=0.906029;MQ0F=0 PL:DP:DV 0,21,184:7:0 0,15,138:5:0 0,18,191:6:0 17 3422 . A 0 . DP=18;I16=9,8,0,0,576,20954,0,0,989,58441,0,0,302,6702,0,0;QS=3,0;MQSB=0.91051;MQ0F=0 PL:DP:DV 0,18,167:6:0 0,15,130:5:0 0,18,188:6:0 17 3423 . G 0 . DP=18;I16=9,9,0,0,651,24391,0,0,1049,62041,0,0,305,6747,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,21,200:7:0 0,15,149:5:0 0,18,183:6:0 17 3424 . G 0 . DP=18;I16=8,8,0,0,555,20175,0,0,929,54841,0,0,275,6105,0,0;QS=3,0;MQSB=0.915545;MQ0F=0 PL:DP:DV 0,18,161:6:0 0,15,141:5:0 0,15,169:5:0 17 3425 . C 0 . DP=18;I16=9,8,0,0,594,21676,0,0,1020,61200,0,0,307,6779,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,168:6:0 0,15,142:5:0 0,18,186:6:0 17 3426 . A 0 . DP=18;I16=9,8,0,0,633,23967,0,0,1020,61200,0,0,308,6774,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,18,172:6:0 0,15,151:5:0 0,18,202:6:0 17 3427 . G 0 . DP=18;I16=9,9,0,0,655,24639,0,0,1049,62041,0,0,315,6823,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,21,209:7:0 0,15,144:5:0 0,18,184:6:0 17 3428 . A 0 . DP=18;I16=9,7,0,0,573,21235,0,0,960,57600,0,0,305,6793,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,15,158:5:0 0,15,147:5:0 0,18,178:6:0 17 3429 . C 0 . DP=17;I16=8,9,0,0,516,16610,0,0,989,58441,0,0,320,6930,0,0;QS=3,0;MQSB=0.928603;MQ0F=0 PL:DP:DV 0,21,176:7:0 0,15,125:5:0 0,15,124:5:0 17 3430 . G 0 . DP=18;I16=9,9,0,0,539,17155,0,0,1049,62041,0,0,323,7011,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,24,181:8:0 0,15,113:5:0 0,15,144:5:0 17 3431 . T 0 . DP=18;I16=9,9,0,0,651,24101,0,0,1049,62041,0,0,327,7111,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,24,210:8:0 0,15,139:5:0 0,15,166:5:0 17 3432 . T 0 . DP=18;I16=8,9,0,0,577,20601,0,0,989,58441,0,0,306,6606,0,0;QS=3,0;MQSB=0.928603;MQ0F=0 PL:DP:DV 0,21,162:7:0 0,15,144:5:0 0,15,165:5:0 17 3433 . G 0 . DP=18;I16=9,9,0,0,623,22589,0,0,1049,62041,0,0,334,7320,0,0;QS=3,0;MQSB=0.924089;MQ0F=0 PL:DP:DV 0,24,199:8:0 0,15,138:5:0 0,15,164:5:0 17 3434 . C 0 . DP=18;I16=10,7,0,0,582,20838,0,0,1020,61200,0,0,324,7208,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,208:8:0 0,15,130:5:0 0,12,137:4:0 17 3435 . A 0 . DP=18;I16=10,8,0,0,595,21619,0,0,1049,62041,0,0,341,7453,0,0;QS=3,0;MQSB=0.906029;MQ0F=0 PL:DP:DV 0,27,185:9:0 0,15,150:5:0 0,12,151:4:0 17 3436 . G 0 . DP=18;I16=10,8,0,0,617,22277,0,0,1049,62041,0,0,345,7549,0,0;QS=3,0;MQSB=0.906029;MQ0F=0 PL:DP:DV 0,27,218:9:0 0,15,146:5:0 0,12,133:4:0 17 3437 . T G, 0 . DP=18;I16=10,7,0,1,594,21168,16,256,1020,61200,29,841,333,7409,16,256;QS=2.94286,0.0571429,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.906029;BQB=1;MQ0F=0 PL:DP:DV 0,11,188,24,191,195:9:1 0,15,148,15,148,148:5:0 0,12,132,12,132,132:4:0 17 3438 . G 0 . DP=18;I16=10,7,0,0,622,23296,0,0,1020,61200,0,0,335,7461,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,215:8:0 0,15,152:5:0 0,12,140:4:0 17 3439 . A 0 . DP=18;I16=10,8,0,0,619,22965,0,0,1049,62041,0,0,355,7853,0,0;QS=3,0;MQSB=0.906029;MQ0F=0 PL:DP:DV 0,27,215:9:0 0,15,153:5:0 0,12,132:4:0 17 3440 . G 0 . DP=18;I16=10,7,0,0,610,22660,0,0,1020,61200,0,0,339,7613,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,221:8:0 0,15,143:5:0 0,12,131:4:0 17 3441 . C 0 . DP=18;I16=10,6,0,0,585,22165,0,0,960,57600,0,0,315,7037,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,226:8:0 0,15,140:5:0 0,9,115:3:0 17 3442 . T 0 . DP=20;I16=11,9,0,0,670,24716,0,0,1138,66482,0,0,362,8166,0,0;QS=3,0;MQSB=0.997118;MQ0F=0 PL:DP:DV 0,33,248:11:0 0,15,143:5:0 0,12,128:4:0 17 3443 . G 0 . DP=20;I16=11,9,0,0,708,26092,0,0,1138,66482,0,0,366,8288,0,0;QS=3,0;MQSB=0.997118;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,143:5:0 0,12,137:4:0 17 3444 . A 0 . DP=20;I16=11,9,0,0,698,25978,0,0,1138,66482,0,0,369,8379,0,0;QS=3,0;MQSB=0.997118;MQ0F=0 PL:DP:DV 0,33,244:11:0 0,15,161:5:0 0,12,131:4:0 17 3445 . G 0 . DP=20;I16=11,9,0,0,718,26590,0,0,1138,66482,0,0,372,8488,0,0;QS=3,0;MQSB=0.997118;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,153:5:0 0,12,132:4:0 17 3446 . A 0 . DP=20;I16=10,8,0,0,635,23323,0,0,1049,62041,0,0,325,7365,0,0;QS=3,0;MQSB=0.938795;MQ0F=0 PL:DP:DV 0,27,222:9:0 0,15,154:5:0 0,12,131:4:0 17 3447 . T 0 . DP=20;I16=11,8,0,0,669,24331,0,0,1109,65641,0,0,352,8084,0,0;QS=3,0;MQSB=0.946915;MQ0F=0 PL:DP:DV 0,30,230:10:0 0,15,150:5:0 0,12,137:4:0 17 3448 . C 0 . DP=19;I16=10,8,0,0,641,23693,0,0,1049,62041,0,0,355,8193,0,0;QS=3,0;MQSB=0.938795;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,113:4:0 0,12,128:4:0 17 3449 . A 0 . DP=19;I16=10,8,0,0,565,19185,0,0,1049,62041,0,0,357,8265,0,0;QS=3,0;MQSB=0.938795;MQ0F=0 PL:DP:DV 0,30,217:10:0 0,12,111:4:0 0,12,119:4:0 17 3450 . C 0 . DP=18;I16=8,8,0,0,520,17846,0,0,898,52082,0,0,334,7674,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,30,229:10:0 0,6,70:2:0 0,12,106:4:0 17 3451 . G 0 . DP=18;I16=10,7,0,0,528,17178,0,0,989,58441,0,0,361,8345,0,0;QS=3,0;MQSB=0.943335;MQ0F=0 PL:DP:DV 0,30,226:10:0 0,9,56:3:0 0,12,123:4:0 17 3452 . C 0 . DP=18;I16=10,8,0,0,657,24597,0,0,1018,59282,0,0,388,9028,0,0;QS=3,0;MQSB=0.99606;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,84:3:0 0,12,128:4:0 17 3453 . C 0 . DP=18;I16=10,8,0,0,663,24951,0,0,1018,59282,0,0,390,9098,0,0;QS=3,0;MQSB=0.99606;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,90:3:0 0,12,125:4:0 17 3454 . A 0 . DP=18;I16=10,8,0,0,614,21898,0,0,1018,59282,0,0,392,9180,0,0;QS=3,0;MQSB=0.99606;MQ0F=0 PL:DP:DV 0,33,251:11:0 0,9,90:3:0 0,12,128:4:0 17 3455 . C 0 . DP=18;I16=10,8,0,0,650,23968,0,0,1018,59282,0,0,394,9274,0,0;QS=3,0;MQSB=0.99606;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,90:3:0 0,12,127:4:0 17 3456 . T 0 . DP=18;I16=10,8,0,0,607,21961,0,0,1018,59282,0,0,395,9329,0,0;QS=3,0;MQSB=0.99606;MQ0F=0 PL:DP:DV 0,33,243:11:0 0,9,88:3:0 0,12,135:4:0 17 3457 . G 0 . DP=18;I16=9,7,0,0,549,19861,0,0,898,52082,0,0,346,8144,0,0;QS=3,0;MQSB=0.994413;MQ0F=0 PL:DP:DV 0,33,254:11:0 0,6,63:2:0 0,9,107:3:0 17 3458 . C 0 . DP=18;I16=10,8,0,0,638,23236,0,0,1018,59282,0,0,397,9469,0,0;QS=3,0;MQSB=0.99606;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,81:3:0 0,12,125:4:0 17 3459 . A C, 0 . DP=17;I16=9,7,0,1,520,18390,22,484,929,54841,29,841,372,8830,25,625;QS=2.92971,0.0702875,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.998843;BQB=1;MQ0F=0 PL:DP:DV 0,8,201,27,204,213:10:1 0,9,84,9,84,84:3:0 0,12,116,12,116,116:4:0 17 3460 . C 0 . DP=17;I16=8,7,0,0,554,20952,0,0,869,51241,0,0,345,8103,0,0;QS=3,0;MQSB=0.921243;MQ0F=0 PL:DP:DV 0,27,244:9:0 0,6,65:2:0 0,12,131:4:0 17 3461 . T 0 . DP=17;I16=9,7,0,0,591,22805,0,0,929,54841,0,0,368,8638,0,0;QS=3,0;MQSB=0.933674;MQ0F=0 PL:DP:DV 0,27,231:9:0 0,9,103:3:0 0,12,133:4:0 17 3462 . C 0 . DP=18;I16=9,9,0,0,672,25486,0,0,1018,59282,0,0,391,9185,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,90:3:0 0,12,130:4:0 17 3463 . C 0 . DP=18;I16=8,9,0,0,584,21458,0,0,958,55682,0,0,369,8671,0,0;QS=3,0;MQSB=0.998843;MQ0F=0 PL:DP:DV 0,30,244:10:0 0,9,86:3:0 0,12,132:4:0 17 3464 . A 0 . DP=18;I16=9,9,0,0,628,23490,0,0,1018,59282,0,0,387,8973,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,244:11:0 0,9,96:3:0 0,12,142:4:0 17 3465 . G 0 . DP=19;I16=9,9,0,0,642,24008,0,0,1018,59282,0,0,384,8842,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,91:3:0 0,12,135:4:0 17 3466 . C 0 . DP=20;I16=9,10,0,0,682,25218,0,0,1047,60123,0,0,378,8588,0,0;QS=3,0;MQSB=0.904084;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,9,101:3:0 0,15,147:5:0 17 3467 . C 0 . DP=20;I16=10,9,0,0,685,25919,0,0,1047,60123,0,0,397,9139,0,0;QS=3,0;MQSB=0.948064;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,12,115:4:0 0,15,154:5:0 17 3468 . T 0 . DP=20;I16=10,9,0,0,672,25426,0,0,1047,60123,0,0,374,8410,0,0;QS=3,0;MQSB=0.948064;MQ0F=0 PL:DP:DV 0,30,246:10:0 0,12,126:4:0 0,15,164:5:0 17 3469 . G 0 . DP=21;I16=10,11,0,0,788,30064,0,0,1167,67323,0,0,396,8924,0,0;QS=3,0;MQSB=0.914611;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,146:5:0 0,15,163:5:0 17 3470 . G 0 . DP=22;I16=11,11,0,0,796,29754,0,0,1196,68164,0,0,393,8781,0,0;QS=3,0;MQSB=0.770381;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,131:5:0 0,15,161:5:0 17 3471 . G 0 . DP=23;I16=12,11,0,0,801,29083,0,0,1256,71764,0,0,391,8657,0,0;QS=3,0;MQSB=0.811552;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,133:5:0 0,15,151:5:0 17 3472 . C 0 . DP=23;I16=12,11,0,0,757,26535,0,0,1256,71764,0,0,390,8554,0,0;QS=3,0;MQSB=0.811552;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,145:5:0 0,15,136:5:0 17 3473 . A 0 . DP=23;I16=11,11,0,0,777,28175,0,0,1196,68164,0,0,379,8373,0,0;QS=3,0;MQSB=0.770381;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,148:5:0 0,15,153:5:0 17 3474 . A 0 . DP=23;I16=12,11,0,0,807,29181,0,0,1256,71764,0,0,388,8414,0,0;QS=3,0;MQSB=0.811552;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,150:5:0 0,15,155:5:0 17 3475 . C 0 . DP=22;I16=12,10,0,0,737,25849,0,0,1196,68164,0,0,387,8327,0,0;QS=3,0;MQSB=0.838545;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,137:5:0 0,15,143:5:0 17 3476 . A 0 . DP=22;I16=12,10,0,0,790,29312,0,0,1196,68164,0,0,386,8262,0,0;QS=3,0;MQSB=0.838545;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,149:5:0 0,15,154:5:0 17 3477 . G 0 . DP=22;I16=11,10,0,0,755,28237,0,0,1136,64564,0,0,363,7735,0,0;QS=3,0;MQSB=0.799507;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,126:4:0 0,15,153:5:0 17 3478 . A 0 . DP=25;I16=13,11,0,0,857,31637,0,0,1316,75364,0,0,384,8198,0,0;QS=3,0;MQSB=0.845496;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,167:6:0 0,18,190:6:0 17 3479 . G 0 . DP=25;I16=12,10,0,0,783,28623,0,0,1227,70923,0,0,355,7701,0,0;QS=3,0;MQSB=0.613159;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,152:5:0 0,18,160:6:0 17 3480 . T 0 . DP=25;I16=13,11,0,0,788,27124,0,0,1316,75364,0,0,393,8531,0,0;QS=3,0;MQSB=0.845496;MQ0F=0 PL:DP:DV 0,33,249:11:0 0,21,182:7:0 0,18,155:6:0 17 3481 . A 0 . DP=25;I16=12,12,0,0,858,30884,0,0,1347,78123,0,0,397,8685,0,0;QS=3,0;MQSB=0.941765;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,194:7:0 0,15,148:5:0 17 3482 . A 0 . DP=25;I16=13,12,0,0,927,35013,0,0,1376,78964,0,0,412,8942,0,0;QS=3,0;MQSB=0.822311;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,202:7:0 0,18,182:6:0 17 3483 . G 0 . DP=25;I16=12,12,0,0,794,27410,0,0,1316,75364,0,0,412,9002,0,0;QS=3,0;MQSB=0.786628;MQ0F=0 PL:DP:DV 0,33,253:11:0 0,21,183:7:0 0,18,158:6:0 17 3484 . A C, 0 . DP=24;I16=12,9,0,1,710,25220,14,196,1198,70082,60,3600,346,7514,25,625;QS=2.93665,0.0633484,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.804615;BQB=1;MQ0F=0 PL:DP:DV 0,30,227,30,227,227:10:0 0,7,156,18,159,162:7:1 0,15,133,15,133,133:5:0 17 3485 . C 0 . DP=23;I16=11,11,0,0,766,27656,0,0,1196,68164,0,0,393,8573,0,0;QS=3,0;MQSB=0.770381;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,163:6:0 0,15,120:5:0 17 3486 . T 0 . DP=24;I16=12,10,0,0,799,30529,0,0,1196,68164,0,0,398,8756,0,0;QS=3,0;MQSB=0.838545;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,21,198:7:0 0,15,128:5:0 17 3487 . C 0 . DP=24;I16=10,11,0,0,724,27586,0,0,1167,67323,0,0,393,8905,0,0;QS=3,0;MQSB=0.914611;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,162:6:0 0,12,92:4:0 17 3488 . T 0 . DP=24;I16=12,10,0,0,742,27454,0,0,1196,68164,0,0,429,9571,0,0;QS=3,0;MQSB=0.838545;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,21,172:7:0 0,12,98:4:0 17 3489 . G 0 . DP=25;I16=13,11,0,0,778,28994,0,0,1285,72605,0,0,433,9675,0,0;QS=3,0;MQSB=0.97965;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,24,177:8:0 0,12,75:4:0 17 3490 . T C, 0 . DP=27;I16=14,10,1,0,806,28886,25,625,1254,69846,60,3600,427,9659,12,144;QS=2.90775,0.0922509,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.962269;BQB=1;MQ0F=0 PL:DP:DV 0,39,255,39,255,255:13:0 0,2,168,24,171,183:9:1 0,9,85,9,85,85:3:0 17 3491 . C 0 . DP=28;I16=15,11,0,0,864,31232,0,0,1405,79805,0,0,445,9903,0,0;QS=3,0;MQSB=0.753395;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,197:9:0 0,9,78:3:0 17 3492 . T 0 . DP=28;I16=15,11,0,0,852,31516,0,0,1405,79805,0,0,452,9986,0,0;QS=3,0;MQSB=0.753395;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,212:9:0 0,9,96:3:0 17 3493 . C 0 . DP=28;I16=15,12,0,0,891,32181,0,0,1434,80646,0,0,464,10124,0,0;QS=3,0;MQSB=0.908075;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,27,214:9:0 0,9,80:3:0 17 3493 . CAAAAAAAAAAAAA CAAAAAAAAAAAA,CAAAAAAAAAAAAAA 0 . INDEL;IDV=2;IMF=0.125;DP=28;I16=9,9,0,2,443,11071,47,1129,1049,62041,89,4441,320,7120,30,650;QS=2.81818,0.0909091,0.0909091;VDB=0.504964;SGB=0.346553;MQSB=0.997118;MQ0F=0 PL:DP:DV 0,19,37,19,40,37:11:2 0,18,39,18,39,39:6:0 0,9,23,9,23,23:3:0 17 3494 . A 0 . DP=30;I16=15,12,0,0,970,37408,0,0,1465,83405,0,0,440,9568,0,0;QS=3,0;MQSB=0.723173;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,220:9:0 0,12,117:4:0 17 3495 . A 0 . DP=31;I16=15,13,0,0,966,36178,0,0,1525,87005,0,0,472,10270,0,0;QS=3,0;MQSB=0.695496;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,210:9:0 0,15,129:5:0 17 3496 . A 0 . DP=31;I16=15,13,0,0,974,36928,0,0,1525,87005,0,0,477,10281,0,0;QS=3,0;MQSB=0.695496;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,207:9:0 0,15,138:5:0 17 3497 . A 0 . DP=32;I16=14,14,0,0,1015,39525,0,0,1525,87005,0,0,460,9834,0,0;QS=3,0;MQSB=0.62781;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,208:8:0 0,18,148:6:0 17 3498 . A 0 . DP=32;I16=14,14,0,0,982,37264,0,0,1556,89764,0,0,460,9628,0,0;QS=3,0;MQSB=0.813104;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,27,220:9:0 0,15,115:5:0 17 3499 . A 0 . DP=32;I16=14,14,0,0,1024,40072,0,0,1525,87005,0,0,489,10267,0,0;QS=3,0;MQSB=0.62781;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,222:8:0 0,18,144:6:0 17 3500 . A 0 . DP=31;I16=14,14,0,0,995,38097,0,0,1525,87005,0,0,494,10316,0,0;QS=3,0;MQSB=0.62781;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,214:8:0 0,18,150:6:0 17 3501 . A 0 . DP=31;I16=14,14,0,0,1017,39845,0,0,1525,87005,0,0,499,10399,0,0;QS=3,0;MQSB=0.62781;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,221:8:0 0,18,149:6:0 17 3502 . A 0 . DP=31;I16=14,14,0,0,1051,41955,0,0,1525,87005,0,0,504,10516,0,0;QS=3,0;MQSB=0.62781;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,223:8:0 0,18,154:6:0 17 3503 . A 0 . DP=31;I16=14,14,0,0,1038,40832,0,0,1525,87005,0,0,509,10667,0,0;QS=3,0;MQSB=0.62781;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,229:8:0 0,18,155:6:0 17 3504 . A 0 . DP=31;I16=14,14,0,0,1037,41235,0,0,1525,87005,0,0,512,10750,0,0;QS=3,0;MQSB=0.62781;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,229:8:0 0,18,145:6:0 17 3505 . A 0 . DP=31;I16=14,14,0,0,1039,40959,0,0,1525,87005,0,0,514,10814,0,0;QS=3,0;MQSB=0.62781;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,24,233:8:0 0,18,156:6:0 17 3506 . A 0 . DP=32;I16=11,13,0,0,889,34265,0,0,1378,80882,0,0,427,9079,0,0;QS=3,0;MQSB=0.998323;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,201:6:0 0,18,158:6:0 17 3507 . T 0 . DP=31;I16=10,17,0,0,995,38431,0,0,1527,88923,0,0,493,10499,0,0;QS=3,0;MQSB=0.997168;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,187:6:0 0,18,153:6:0 17 3508 . C 0 . DP=31;I16=10,17,0,0,997,40091,0,0,1527,88923,0,0,499,10675,0,0;QS=3,0;MQSB=0.997168;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,160:6:0 0,15,119:5:0 17 3509 . A 0 . DP=30;I16=11,17,0,0,995,37147,0,0,1556,89764,0,0,529,11459,0,0;QS=3,0;MQSB=0.960952;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,175:6:0 0,15,125:5:0 17 3510 . C A, 0 . DP=29;I16=9,17,1,0,959,37997,40,1600,1498,88082,29,841,483,10351,25,625;QS=2.95246,0.047541,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.997168;BQB=1;MQ0F=0 PL:DP:DV 0,19,255,45,255,255:16:1 0,18,150,18,150,150:6:0 0,15,119,15,119,119:5:0 17 3511 . A 0 . DP=32;I16=11,17,0,0,923,33125,0,0,1587,92523,0,0,512,11150,0,0;QS=3,0;MQSB=0.993109;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,169:6:0 0,15,115:5:0 17 3512 . C 0 . DP=32;I16=10,18,0,0,1058,42208,0,0,1618,95282,0,0,493,10733,0,0;QS=3,0;MQSB=0.891417;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,181:6:0 0,15,131:5:0 17 3513 . C A, 0 . DP=32;I16=10,18,1,0,1074,42406,13,169,1618,95282,29,841,498,10926,25,625;QS=2.98,0.02,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.995968;BQB=1;MQ0F=0 PL:DP:DV 0,39,255,51,255,255:18:1 0,18,190,18,190,190:6:0 0,15,136,15,136,136:5:0 17 3514 . A 0 . DP=33;I16=11,19,0,0,1101,42213,0,0,1707,99723,0,0,528,11778,0,0;QS=3,0;MQSB=0.997918;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,199:7:0 0,15,132:5:0 17 3515 . T 0 . DP=33;I16=12,19,0,0,1130,43380,0,0,1736,100564,0,0,557,12563,0,0;QS=3,0;MQSB=0.960505;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,192:7:0 0,15,126:5:0 17 3516 . T 0 . DP=34;I16=14,17,0,0,1147,44331,0,0,1767,103323,0,0,536,12078,0,0;QS=3,0;MQSB=0.924242;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,203:7:0 0,15,133:5:0 17 3517 . T 0 . DP=34;I16=14,18,0,0,1183,46549,0,0,1796,104164,0,0,565,12773,0,0;QS=3,0;MQSB=0.988522;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,21,210:7:0 0,15,153:5:0 17 3518 . T 0 . DP=34;I16=14,18,0,0,1165,44867,0,0,1796,104164,0,0,568,12826,0,0;QS=3,0;MQSB=0.988522;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,21,203:7:0 0,15,141:5:0 17 3519 . G 0 . DP=33;I16=13,18,0,0,1158,46678,0,0,1736,100564,0,0,572,12912,0,0;QS=3,0;MQSB=0.980167;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,18,189:6:0 0,15,154:5:0 17 3520 . G 0 . DP=33;I16=12,18,0,0,1111,42471,0,0,1707,99723,0,0,552,12438,0,0;QS=3,0;MQSB=0.991968;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,18,183:6:0 0,15,151:5:0 17 3521 . C 0 . DP=32;I16=11,17,0,0,1077,43755,0,0,1649,98041,0,0,530,11850,0,0;QS=3,0;MQSB=0.967085;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,185:6:0 0,15,143:5:0 17 3522 . T 0 . DP=32;I16=12,17,0,0,1125,46329,0,0,1678,98882,0,0,552,12274,0,0;QS=3,0;MQSB=0.993891;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,199:6:0 0,15,158:5:0 17 3523 . T 0 . DP=31;I16=11,16,0,0,996,38204,0,0,1558,91682,0,0,544,12286,0,0;QS=3,0;MQSB=0.992419;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,193:6:0 0,15,145:5:0 17 3524 . C 0 . DP=31;I16=11,16,0,0,1067,43883,0,0,1589,94441,0,0,538,11960,0,0;QS=3,0;MQSB=0.96384;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,192:6:0 0,15,152:5:0 17 3525 . A 0 . DP=31;I16=13,16,0,0,1132,46402,0,0,1678,98882,0,0,556,12250,0,0;QS=3,0;MQSB=0.997839;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,200:6:0 0,15,165:5:0 17 3526 . G 0 . DP=31;I16=12,16,0,0,1090,43912,0,0,1649,98041,0,0,543,12053,0,0;QS=3,0;MQSB=0.96195;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,181:6:0 0,15,147:5:0 17 3527 . A 0 . DP=31;I16=13,16,0,0,1068,41740,0,0,1678,98882,0,0,560,12334,0,0;QS=3,0;MQSB=0.997839;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,170:6:0 0,15,157:5:0 17 3528 . T 0 . DP=31;I16=13,16,0,0,1076,41782,0,0,1678,98882,0,0,561,12369,0,0;QS=3,0;MQSB=0.997839;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,193:6:0 0,15,152:5:0 17 3529 . T 0 . DP=31;I16=12,16,0,0,1016,38674,0,0,1649,98041,0,0,550,12290,0,0;QS=3,0;MQSB=0.96195;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,184:6:0 0,15,154:5:0 17 3530 . G 0 . DP=30;I16=12,17,0,0,1070,40570,0,0,1709,101641,0,0,578,13032,0,0;QS=3,0;MQSB=0.965321;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,176:6:0 0,18,175:6:0 17 3531 . C A, 0 . DP=31;I16=13,17,1,0,1080,40304,38,1444,1738,102482,29,841,607,13801,10,100;QS=2.95773,0.0422741,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.924242;BQB=1;MQ0F=0 PL:DP:DV 0,28,255,54,255,255:19:1 0,18,172,18,172,172:6:0 0,18,175,18,175,175:6:0 17 3532 . A 0 . DP=31;I16=14,17,0,0,1136,42380,0,0,1767,103323,0,0,619,14003,0,0;QS=3,0;MQSB=0.924242;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,18,180:6:0 0,18,184:6:0 17 3533 . T 0 . DP=31;I16=13,17,0,0,1074,39206,0,0,1738,102482,0,0,595,13457,0,0;QS=3,0;MQSB=0.996503;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,180:6:0 0,18,176:6:0 17 3534 . A 0 . DP=30;I16=12,17,0,0,1014,36168,0,0,1678,98882,0,0,597,13561,0,0;QS=3,0;MQSB=0.993891;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,165:6:0 0,18,175:6:0 17 3535 . T 0 . DP=31;I16=14,17,0,0,1042,36456,0,0,1767,103323,0,0,624,14314,0,0;QS=3,0;MQSB=0.924242;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,18,162:6:0 0,18,164:6:0 17 3536 . C 0 . DP=31;I16=13,17,0,0,1102,41182,0,0,1738,102482,0,0,602,13842,0,0;QS=3,0;MQSB=0.996503;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,178:6:0 0,18,180:6:0 17 3537 . C 0 . DP=32;I16=13,17,0,0,1142,43828,0,0,1769,105241,0,0,598,13854,0,0;QS=3,0;MQSB=0.963674;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,194:7:0 0,18,185:6:0 17 3538 . T 0 . DP=32;I16=14,17,0,0,1151,43471,0,0,1798,106082,0,0,603,13923,0,0;QS=3,0;MQSB=0.998229;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,211:7:0 0,18,185:6:0 17 3539 . C 0 . DP=32;I16=12,17,0,0,1077,40959,0,0,1709,101641,0,0,600,13994,0,0;QS=3,0;MQSB=0.965321;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,170:6:0 0,18,173:6:0 17 3540 . C 0 . DP=31;I16=13,17,0,0,1134,43546,0,0,1769,105241,0,0,603,14055,0,0;QS=3,0;MQSB=0.963674;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,209:7:0 0,18,172:6:0 17 3541 . T 0 . DP=32;I16=13,18,0,0,1143,42901,0,0,1829,108841,0,0,604,14134,0,0;QS=3,0;MQSB=0.966712;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,224:8:0 0,18,187:6:0 17 3542 . G 0 . DP=32;I16=13,18,0,0,1152,43714,0,0,1829,108841,0,0,604,14134,0,0;QS=3,0;MQSB=0.966712;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,223:8:0 0,18,172:6:0 17 3543 . C 0 . DP=31;I16=13,17,0,0,1142,43818,0,0,1769,105241,0,0,605,14153,0,0;QS=3,0;MQSB=0.963674;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,24,221:8:0 0,18,188:6:0 17 3544 . A 0 . DP=31;I16=13,17,0,0,1089,40065,0,0,1769,105241,0,0,606,14190,0,0;QS=3,0;MQSB=0.963674;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,24,221:8:0 0,18,173:6:0 17 3545 . A 0 . DP=30;I16=13,16,0,0,1118,43688,0,0,1709,101641,0,0,607,14195,0,0;QS=3,0;MQSB=0.960189;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,21,228:7:0 0,18,196:6:0 17 3546 . G 0 . DP=30;I16=14,16,0,0,1131,43425,0,0,1738,102482,0,0,630,14698,0,0;QS=3,0;MQSB=0.999136;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,206:7:0 0,18,179:6:0 17 3547 . G 0 . DP=31;I16=13,17,0,0,1076,39798,0,0,1769,105241,0,0,607,14163,0,0;QS=3,0;MQSB=0.963674;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,189:7:0 0,18,187:6:0 17 3548 . A 0 . DP=31;I16=13,17,0,0,1071,39059,0,0,1769,105241,0,0,608,14178,0,0;QS=3,0;MQSB=0.963674;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,208:7:0 0,18,179:6:0 17 3549 . T 0 . DP=30;I16=13,16,0,0,1008,35928,0,0,1678,98882,0,0,605,13989,0,0;QS=3,0;MQSB=0.997839;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,180:6:0 0,15,152:5:0 17 3550 . A 0 . DP=30;I16=13,16,0,0,1061,39371,0,0,1709,101641,0,0,612,14270,0,0;QS=3,0;MQSB=0.960189;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,194:7:0 0,15,173:5:0 17 3551 . T 0 . DP=30;I16=13,16,0,0,1026,36844,0,0,1709,101641,0,0,613,14295,0,0;QS=3,0;MQSB=0.960189;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,192:7:0 0,15,162:5:0 17 3552 . A 0 . DP=30;I16=14,16,0,0,1080,39588,0,0,1738,102482,0,0,631,14627,0,0;QS=3,0;MQSB=0.999136;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,202:7:0 0,15,158:5:0 17 3553 . T A, 0 . DP=30;I16=13,16,1,0,1047,38333,20,400,1709,101641,29,841,616,14398,16,256;QS=2.96795,0.0320513,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.999136;BQB=1;MQ0F=0 PL:DP:DV 0,34,255,51,255,255:18:1 0,18,178,18,178,178:6:0 0,18,183,18,183,183:6:0 17 3554 . A 0 . DP=30;I16=14,16,0,0,1063,38413,0,0,1738,102482,0,0,632,14602,0,0;QS=3,0;MQSB=0.999136;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,185:6:0 0,18,179:6:0 17 3555 . C 0 . DP=30;I16=14,16,0,0,1021,35781,0,0,1738,102482,0,0,632,14574,0,0;QS=3,0;MQSB=0.999136;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,169:6:0 0,18,163:6:0 17 3556 . G 0 . DP=30;I16=13,16,0,0,1014,36426,0,0,1709,101641,0,0,618,14350,0,0;QS=3,0;MQSB=0.960189;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,168:6:0 0,18,173:6:0 17 3557 . C 0 . DP=31;I16=14,16,0,0,1001,34919,0,0,1769,105241,0,0,618,14342,0,0;QS=3,0;MQSB=0.958545;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,140:6:0 0,18,165:6:0 17 3558 . G 0 . DP=31;I16=15,16,0,0,1036,35974,0,0,1798,106082,0,0,630,14476,0,0;QS=3,0;MQSB=0.999805;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,18,159:6:0 0,18,171:6:0 17 3559 . T 0 . DP=30;I16=14,16,0,0,1086,40202,0,0,1769,105241,0,0,619,14341,0,0;QS=3,0;MQSB=0.958545;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,171:6:0 0,18,192:6:0 17 3560 . G 0 . DP=31;I16=14,16,0,0,1151,45035,0,0,1738,102482,0,0,611,14127,0,0;QS=3,0;MQSB=0.999136;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,165:5:0 0,18,194:6:0 17 3561 . A 0 . DP=31;I16=15,16,0,0,1147,43431,0,0,1798,106082,0,0,626,14366,0,0;QS=3,0;MQSB=0.999805;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,18,186:6:0 0,18,196:6:0 17 3562 . A 0 . DP=31;I16=16,15,0,0,1157,43859,0,0,1798,106082,0,0,623,14257,0,0;QS=3,0;MQSB=0.999805;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,192:6:0 0,21,213:7:0 17 3563 . A 0 . DP=31;I16=16,15,0,0,1162,44010,0,0,1798,106082,0,0,620,14124,0,0;QS=3,0;MQSB=0.999805;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,192:6:0 0,21,216:7:0 17 3564 . T 0 . DP=31;I16=15,15,0,0,1132,43298,0,0,1769,105241,0,0,610,13932,0,0;QS=3,0;MQSB=0.952765;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,192:6:0 0,21,214:7:0 17 3565 . T 0 . DP=32;I16=16,16,0,0,1163,42649,0,0,1858,109682,0,0,611,13791,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,18,189:6:0 0,21,212:7:0 17 3566 . C 0 . DP=32;I16=16,16,0,0,1162,43586,0,0,1858,109682,0,0,606,13596,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,18,181:6:0 0,21,209:7:0 17 3567 . A 0 . DP=32;I16=15,16,0,0,1181,45245,0,0,1829,108841,0,0,597,13375,0,0;QS=3,0;MQSB=0.957006;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,192:6:0 0,21,222:7:0 17 3568 . A 0 . DP=32;I16=15,16,0,0,1194,46670,0,0,1829,108841,0,0,592,13200,0,0;QS=3,0;MQSB=0.957006;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,204:6:0 0,21,228:7:0 17 3569 . G 0 . DP=31;I16=15,16,0,0,1164,44544,0,0,1829,108841,0,0,586,13006,0,0;QS=3,0;MQSB=0.957006;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,18,180:6:0 0,21,204:7:0 17 3570 . T 0 . DP=30;I16=14,15,0,0,1029,37527,0,0,1709,101641,0,0,572,12730,0,0;QS=3,0;MQSB=0.954405;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,12,136:4:0 0,21,213:7:0 17 3571 . C 0 . DP=28;I16=13,15,0,0,1066,41192,0,0,1649,98041,0,0,568,12564,0,0;QS=3,0;MQSB=0.956162;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,138:4:0 0,21,215:7:0 17 3572 . A 0 . DP=29;I16=13,16,0,0,1108,42566,0,0,1709,101641,0,0,564,12426,0,0;QS=3,0;MQSB=0.960189;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,15,172:5:0 0,21,218:7:0 17 3573 . A 0 . DP=29;I16=13,16,0,0,1119,43403,0,0,1709,101641,0,0,558,12168,0,0;QS=3,0;MQSB=0.960189;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,15,174:5:0 0,21,220:7:0 17 3574 . T 0 . DP=29;I16=13,16,0,0,1104,42220,0,0,1709,101641,0,0,552,11942,0,0;QS=3,0;MQSB=0.960189;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,15,175:5:0 0,21,218:7:0 17 3575 . G 0 . DP=29;I16=13,16,0,0,1122,43748,0,0,1709,101641,0,0,546,11748,0,0;QS=3,0;MQSB=0.960189;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,15,161:5:0 0,21,212:7:0 17 3576 . A 0 . DP=29;I16=13,16,0,0,1043,38405,0,0,1709,101641,0,0,540,11586,0,0;QS=3,0;MQSB=0.960189;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,15,170:5:0 0,21,213:7:0 17 3577 . C 0 . DP=30;I16=13,17,0,0,1123,42529,0,0,1769,105241,0,0,534,11456,0,0;QS=3,0;MQSB=0.963674;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,15,161:5:0 0,21,211:7:0 17 3578 . A 0 . DP=30;I16=13,17,0,0,1148,44236,0,0,1769,105241,0,0,529,11359,0,0;QS=3,0;MQSB=0.963674;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,15,178:5:0 0,21,216:7:0 17 3579 . A 0 . DP=29;I16=13,16,0,0,1051,38961,0,0,1709,101641,0,0,524,11244,0,0;QS=3,0;MQSB=0.960189;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,15,172:5:0 0,18,192:6:0 17 3580 . A 0 . DP=29;I16=13,16,0,0,1104,42344,0,0,1709,101641,0,0,519,11159,0,0;QS=3,0;MQSB=0.960189;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,15,172:5:0 0,18,201:6:0 17 3581 . T 0 . DP=29;I16=13,16,0,0,1088,41226,0,0,1709,101641,0,0,513,11055,0,0;QS=3,0;MQSB=0.960189;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,15,161:5:0 0,18,199:6:0 17 3582 . C 0 . DP=29;I16=13,16,0,0,1120,43616,0,0,1709,101641,0,0,506,10934,0,0;QS=3,0;MQSB=0.960189;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,15,172:5:0 0,18,201:6:0 17 3583 . A 0 . DP=30;I16=14,16,0,0,1130,43606,0,0,1769,105241,0,0,498,10796,0,0;QS=3,0;MQSB=0.958545;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,15,183:5:0 0,21,213:7:0 17 3584 . G 0 . DP=29;I16=12,16,0,0,1027,38895,0,0,1649,98041,0,0,487,10665,0,0;QS=3,0;MQSB=0.96195;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,12,137:4:0 0,21,209:7:0 17 3585 . A 0 . DP=31;I16=14,17,0,0,1057,37719,0,0,1829,108841,0,0,486,10616,0,0;QS=3,0;MQSB=0.962133;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,15,168:5:0 0,24,209:8:0 17 3586 . A 0 . DP=30;I16=14,15,0,0,1053,40043,0,0,1709,101641,0,0,477,10461,0,0;QS=3,0;MQSB=0.954405;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,174:5:0 0,24,213:8:0 17 3587 . G A, 0 . DP=29;I16=4,7,10,6,426,16884,555,20381,660,39600,960,57600,192,4420,280,5942;QS=1.32665,1.67335,0;VDB=0.902044;SGB=-3.91326;RPB=0.800999;MQB=1;MQSB=1;BQB=0.156944;MQ0F=0 PL:DP:DV 161,0,184,182,205,255:14:7 22,0,118,34,121,148:5:1 212,24,0,212,24,212:8:8 17 3588 . A 0 . DP=29;I16=14,14,0,0,999,36973,0,0,1680,100800,0,0,470,10254,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,166:5:0 0,24,215:8:0 17 3589 . A 0 . DP=28;I16=14,13,0,0,967,35935,0,0,1620,97200,0,0,467,10173,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,166:5:0 0,24,206:8:0 17 3590 . A 0 . DP=27;I16=13,13,0,0,948,35860,0,0,1560,93600,0,0,464,10072,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,146:4:0 0,24,211:8:0 17 3591 . A 0 . DP=26;I16=13,13,0,0,913,33539,0,0,1560,93600,0,0,459,9901,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,144:4:0 0,24,212:8:0 17 3592 . A 0 . DP=26;I16=13,13,0,0,893,32087,0,0,1560,93600,0,0,453,9711,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,143:4:0 0,24,199:8:0 17 3593 . A 0 . DP=26;I16=13,13,0,0,891,31363,0,0,1560,93600,0,0,447,9553,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,137:4:0 0,24,198:8:0 17 3594 . C 0 . DP=25;I16=12,12,0,0,910,34868,0,0,1440,86400,0,0,426,9170,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,142:4:0 0,24,216:8:0 17 3595 . A 0 . DP=24;I16=13,11,0,0,918,35274,0,0,1440,86400,0,0,438,9328,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,143:4:0 0,21,199:7:0 17 3596 . T 0 . DP=24;I16=13,11,0,0,837,30077,0,0,1440,86400,0,0,434,9258,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,138:4:0 0,21,188:7:0 17 3597 . A 0 . DP=24;I16=13,11,0,0,893,33549,0,0,1440,86400,0,0,430,9216,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,132:4:0 0,21,204:7:0 17 3598 . T 0 . DP=23;I16=12,10,0,0,778,28226,0,0,1320,79200,0,0,415,9005,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,140:4:0 0,21,162:7:0 17 3599 . A 0 . DP=23;I16=13,10,0,0,859,32403,0,0,1380,82800,0,0,425,9105,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,142:4:0 0,21,182:7:0 17 3600 . T 0 . DP=24;I16=14,10,0,0,872,32086,0,0,1435,85825,0,0,422,9036,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,139:4:0 0,21,183:7:0 17 3601 . A 0 . DP=24;I16=14,10,0,0,862,31424,0,0,1435,85825,0,0,420,8994,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,132:4:0 0,21,186:7:0 17 3602 . T 0 . DP=25;I16=15,10,0,0,873,31323,0,0,1495,89425,0,0,418,8980,0,0;QS=3,0;MQSB=0.962269;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,161:5:0 0,21,174:7:0 17 3603 . A 0 . DP=26;I16=15,11,0,0,907,32447,0,0,1555,93025,0,0,416,8944,0,0;QS=3,0;MQSB=0.960078;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,151:5:0 0,24,216:8:0 17 3604 . C 0 . DP=27;I16=16,11,0,0,905,31199,0,0,1615,96625,0,0,415,8937,0,0;QS=3,0;MQSB=0.96384;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,147:6:0 0,24,201:8:0 17 3605 . G 0 . DP=27;I16=15,11,0,0,853,28887,0,0,1555,93025,0,0,393,8477,0,0;QS=3,0;MQSB=0.960078;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,171:6:0 0,21,174:7:0 17 3606 . C 0 . DP=27;I16=15,12,0,0,982,36756,0,0,1615,96625,0,0,415,8967,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,187:6:0 0,27,231:9:0 17 3607 . A 0 . DP=26;I16=15,11,0,0,927,33859,0,0,1555,93025,0,0,417,9005,0,0;QS=3,0;MQSB=0.960078;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,183:6:0 0,27,232:9:0 17 3608 . A 0 . DP=27;I16=14,12,0,0,930,34054,0,0,1555,93025,0,0,394,8450,0,0;QS=3,0;MQSB=0.953497;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,176:6:0 0,24,232:8:0 17 3609 . A 0 . DP=27;I16=15,12,0,0,955,34701,0,0,1615,96625,0,0,421,9127,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,186:6:0 0,27,238:9:0 17 3610 . C 0 . DP=27;I16=14,12,0,0,915,33071,0,0,1555,93025,0,0,397,8537,0,0;QS=3,0;MQSB=0.953497;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,172:6:0 0,24,221:8:0 17 3611 . C 0 . DP=25;I16=13,11,0,0,899,33995,0,0,1435,85825,0,0,398,8502,0,0;QS=3,0;MQSB=0.950498;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,173:6:0 0,24,234:8:0 17 3612 . A 0 . DP=25;I16=14,11,0,0,957,37723,0,0,1495,89425,0,0,424,9118,0,0;QS=3,0;MQSB=0.955682;MQ0F=0 PL:DP:DV 0,30,255:10:0 0,18,194:6:0 0,27,251:9:0 17 3613 . G 0 . DP=26;I16=13,12,0,0,902,33110,0,0,1495,89425,0,0,399,8461,0,0;QS=3,0;MQSB=0.948139;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,172:6:0 0,24,223:8:0 17 3614 . T 0 . DP=26;I16=14,12,0,0,912,33340,0,0,1555,93025,0,0,425,9083,0,0;QS=3,0;MQSB=0.953497;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,156:6:0 0,27,237:9:0 17 3615 . A 0 . DP=26;I16=15,11,0,0,946,35138,0,0,1555,93025,0,0,427,9109,0,0;QS=3,0;MQSB=0.960078;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,176:6:0 0,27,242:9:0 17 3616 . T 0 . DP=26;I16=15,11,0,0,965,36541,0,0,1555,93025,0,0,431,9163,0,0;QS=3,0;MQSB=0.960078;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,185:6:0 0,27,238:9:0 17 3617 . C 0 . DP=25;I16=14,11,0,0,907,33675,0,0,1495,89425,0,0,436,9196,0,0;QS=3,0;MQSB=0.955682;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,176:6:0 0,24,215:8:0 17 3618 . C 0 . DP=25;I16=14,11,0,0,964,37698,0,0,1495,89425,0,0,441,9259,0,0;QS=3,0;MQSB=0.955682;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,190:6:0 0,24,225:8:0 17 3619 . T 0 . DP=25;I16=14,11,0,0,961,37553,0,0,1495,89425,0,0,446,9352,0,0;QS=3,0;MQSB=0.955682;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,194:6:0 0,24,238:8:0 17 3620 . A 0 . DP=25;I16=14,11,0,0,880,31656,0,0,1495,89425,0,0,451,9475,0,0;QS=3,0;MQSB=0.955682;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,178:6:0 0,24,212:8:0 17 3621 . C 0 . DP=25;I16=14,11,0,0,949,36561,0,0,1495,89425,0,0,456,9628,0,0;QS=3,0;MQSB=0.955682;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,18,182:6:0 0,24,230:8:0 17 3622 . T 0 . DP=26;I16=15,11,0,0,983,38067,0,0,1555,93025,0,0,460,9762,0,0;QS=3,0;MQSB=0.960078;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,21,194:7:0 0,24,242:8:0 17 3623 . G 0 . DP=27;I16=16,11,0,0,969,35645,0,0,1615,96625,0,0,465,9929,0,0;QS=3,0;MQSB=0.96384;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,195:7:0 0,24,209:8:0 17 3624 . T 0 . DP=27;I16=15,11,0,0,948,35330,0,0,1555,93025,0,0,448,9596,0,0;QS=3,0;MQSB=0.960078;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,188:6:0 0,24,224:8:0 17 3625 . G 0 . DP=27;I16=14,12,0,0,983,37483,0,0,1555,93025,0,0,453,9735,0,0;QS=3,0;MQSB=0.953497;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,21,210:7:0 0,24,219:8:0 17 3626 . T 0 . DP=28;I16=15,12,0,0,998,37364,0,0,1615,96625,0,0,458,9854,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,21,214:7:0 0,27,229:9:0 17 3627 . G 0 . DP=29;I16=15,13,0,0,999,36375,0,0,1675,100225,0,0,464,10004,0,0;QS=3,0;MQSB=0.956162;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,199:7:0 0,27,224:9:0 17 3628 . T 0 . DP=29;I16=15,13,0,0,1033,38721,0,0,1675,100225,0,0,471,10187,0,0;QS=3,0;MQSB=0.956162;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,206:7:0 0,27,232:9:0 17 3629 . G 0 . DP=29;I16=15,13,0,0,1058,40226,0,0,1675,100225,0,0,476,10304,0,0;QS=3,0;MQSB=0.956162;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,207:7:0 0,27,235:9:0 17 3630 . T 0 . DP=30;I16=15,14,0,0,1052,39072,0,0,1735,103825,0,0,480,10404,0,0;QS=3,0;MQSB=0.954405;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,216:7:0 0,27,223:9:0 17 3631 . C 0 . DP=29;I16=13,14,0,0,884,29476,0,0,1615,96625,0,0,461,9911,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,186:7:0 0,21,179:7:0 17 3632 . G 0 . DP=29;I16=14,14,0,0,914,31068,0,0,1675,100225,0,0,491,10649,0,0;QS=3,0;MQSB=0.949591;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,183:7:0 0,24,181:8:0 17 3633 . T 0 . DP=29;I16=14,14,0,0,1022,38214,0,0,1675,100225,0,0,496,10792,0,0;QS=3,0;MQSB=0.949591;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,216:7:0 0,24,222:8:0 17 3634 . T 0 . DP=29;I16=14,14,0,0,1043,39891,0,0,1675,100225,0,0,500,10914,0,0;QS=3,0;MQSB=0.949591;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,219:7:0 0,24,227:8:0 17 3635 . T 0 . DP=28;I16=13,14,0,0,1029,39507,0,0,1615,96625,0,0,505,11063,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,219:7:0 0,24,224:8:0 17 3636 . G 0 . DP=28;I16=13,14,0,0,1001,37681,0,0,1615,96625,0,0,510,11238,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,201:7:0 0,24,217:8:0 17 3637 . T 0 . DP=27;I16=13,14,0,0,1019,39331,0,0,1615,96625,0,0,515,11439,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,212:7:0 0,24,240:8:0 17 3638 . T 0 . DP=26;I16=12,14,0,0,967,36467,0,0,1555,93025,0,0,520,11616,0,0;QS=3,0;MQSB=0.937241;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,185:6:0 0,24,222:8:0 17 3639 . G 0 . DP=27;I16=13,14,0,0,997,37265,0,0,1615,96625,0,0,524,11768,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,183:6:0 0,27,221:9:0 17 3640 . T 0 . DP=27;I16=13,14,0,0,1001,37533,0,0,1615,96625,0,0,527,11847,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,191:6:0 0,27,229:9:0 17 3641 . G 0 . DP=27;I16=13,14,0,0,977,36379,0,0,1615,96625,0,0,529,11905,0,0;QS=3,0;MQSB=0.94394;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,178:6:0 0,27,224:9:0 17 3642 . T 0 . DP=26;I16=12,13,0,0,931,35169,0,0,1495,89425,0,0,506,11314,0,0;QS=3,0;MQSB=0.939413;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,169:5:0 0,24,225:8:0 17 3643 . T 0 . DP=26;I16=13,13,0,0,968,36820,0,0,1555,93025,0,0,533,11997,0,0;QS=3,0;MQSB=0.945959;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,176:5:0 0,27,230:9:0 17 3644 . T 0 . DP=26;I16=12,13,0,0,961,37477,0,0,1495,89425,0,0,517,11755,0,0;QS=3,0;MQSB=0.939413;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,175:5:0 0,24,236:8:0 17 3645 . T 0 . DP=26;I16=13,13,0,0,980,37508,0,0,1555,93025,0,0,537,12185,0,0;QS=3,0;MQSB=0.945959;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,175:5:0 0,27,239:9:0 17 3646 . C 0 . DP=26;I16=12,13,0,0,766,25026,0,0,1495,89425,0,0,514,11690,0,0;QS=3,0;MQSB=0.939413;MQ0F=0 PL:DP:DV 0,33,234:11:0 0,15,136:5:0 0,27,188:9:0 17 3647 . G 0 . DP=26;I16=12,12,0,0,820,29600,0,0,1435,85825,0,0,492,11218,0,0;QS=3,0;MQSB=0.941765;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,151:5:0 0,21,173:7:0 17 3648 . A 0 . DP=26;I16=13,12,0,0,924,34680,0,0,1495,89425,0,0,519,11919,0,0;QS=3,0;MQSB=0.948139;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,174:5:0 0,24,225:8:0 17 3649 . C 0 . DP=26;I16=14,12,0,0,955,36105,0,0,1555,93025,0,0,545,12593,0,0;QS=3,0;MQSB=0.953497;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,173:5:0 0,27,226:9:0 17 3650 . A 0 . DP=27;I16=15,12,0,0,1033,40511,0,0,1615,96625,0,0,546,12664,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,196:6:0 0,27,246:9:0 17 3651 . G 0 . DP=27;I16=15,12,0,0,1035,40351,0,0,1615,96625,0,0,547,12707,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,194:6:0 0,27,233:9:0 17 3652 . C 0 . DP=29;I16=15,13,0,0,1010,38264,0,0,1675,100225,0,0,521,12047,0,0;QS=3,0;MQSB=0.956162;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,198:6:0 0,27,225:9:0 17 3653 . T 0 . DP=29;I16=16,13,0,0,1109,42919,0,0,1735,103825,0,0,546,12610,0,0;QS=3,0;MQSB=0.960189;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,202:6:0 0,27,239:9:0 17 3654 . G 0 . DP=28;I16=14,12,0,0,981,37447,0,0,1555,93025,0,0,514,11882,0,0;QS=3,0;MQSB=0.953497;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,184:6:0 0,18,180:6:0 17 3655 . T 0 . DP=28;I16=16,12,0,0,999,37029,0,0,1675,100225,0,0,541,12505,0,0;QS=3,0;MQSB=0.96195;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,190:6:0 0,21,179:7:0 17 3656 . C 0 . DP=28;I16=15,12,0,0,963,35883,0,0,1615,96625,0,0,518,11848,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,164:5:0 0,21,191:7:0 17 3657 . C 0 . DP=28;I16=15,12,0,0,923,32223,0,0,1615,96625,0,0,520,11836,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,154:5:0 0,21,192:7:0 17 3658 . G 0 . DP=28;I16=15,12,0,0,876,29960,0,0,1615,96625,0,0,539,12405,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,142:5:0 0,21,163:7:0 17 3659 . T 0 . DP=28;I16=16,12,0,0,996,36682,0,0,1675,100225,0,0,548,12448,0,0;QS=3,0;MQSB=0.96195;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,163:6:0 0,21,186:7:0 17 3660 . G 0 . DP=28;I16=15,12,0,0,997,37571,0,0,1615,96625,0,0,526,11920,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,172:6:0 0,18,171:6:0 17 3661 . T 0 . DP=28;I16=16,12,0,0,1013,37837,0,0,1675,100225,0,0,549,12423,0,0;QS=3,0;MQSB=0.96195;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,180:6:0 0,21,188:7:0 17 3662 . T 0 . DP=28;I16=15,12,0,0,987,36627,0,0,1615,96625,0,0,528,11980,0,0;QS=3,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,182:6:0 0,18,184:6:0 17 3663 . A 0 . DP=29;I16=16,12,0,0,997,36233,0,0,1675,100225,0,0,527,11959,0,0;QS=3,0;MQSB=0.96195;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,170:6:0 0,18,180:6:0 17 3664 . T 0 . DP=29;I16=17,12,0,0,1044,38402,0,0,1735,103825,0,0,550,12490,0,0;QS=3,0;MQSB=0.965321;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,183:6:0 0,21,196:7:0 17 3665 . A 0 . DP=27;I16=16,11,0,0,1017,38535,0,0,1615,96625,0,0,552,12510,0,0;QS=3,0;MQSB=0.96384;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,182:6:0 0,21,208:7:0 17 3666 . A 0 . DP=27;I16=16,11,0,0,1036,40292,0,0,1615,96625,0,0,554,12550,0,0;QS=3,0;MQSB=0.96384;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,193:6:0 0,21,208:7:0 17 3667 . T 0 . DP=27;I16=15,11,0,0,955,35895,0,0,1555,93025,0,0,540,12354,0,0;QS=3,0;MQSB=0.960078;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,189:6:0 0,18,189:6:0 17 3668 . A 0 . DP=27;I16=16,11,0,0,981,36297,0,0,1615,96625,0,0,557,12641,0,0;QS=3,0;MQSB=0.96384;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,182:6:0 0,21,209:7:0 17 3669 . A 0 . DP=27;I16=16,11,0,0,1030,39666,0,0,1615,96625,0,0,558,12694,0,0;QS=3,0;MQSB=0.96384;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,194:6:0 0,21,209:7:0 17 3670 . T 0 . DP=28;I16=17,11,0,0,1057,40245,0,0,1675,100225,0,0,559,12769,0,0;QS=3,0;MQSB=0.967085;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,181:6:0 0,24,220:8:0 17 3671 . T 0 . DP=28;I16=17,11,0,0,1059,40427,0,0,1675,100225,0,0,561,12867,0,0;QS=3,0;MQSB=0.967085;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,193:6:0 0,24,223:8:0 17 3672 . C 0 . DP=28;I16=16,11,0,0,988,37264,0,0,1615,96625,0,0,550,12820,0,0;QS=3,0;MQSB=0.96384;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,169:6:0 0,21,205:7:0 17 3673 . C 0 . DP=27;I16=16,10,0,0,1016,40148,0,0,1555,93025,0,0,528,12314,0,0;QS=3,0;MQSB=0.965874;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,154:5:0 0,24,219:8:0 17 3674 . T 0 . DP=27;I16=17,10,0,0,1035,40645,0,0,1615,96625,0,0,556,13028,0,0;QS=3,0;MQSB=0.968979;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,155:5:0 0,24,228:8:0 17 3675 . C 0 . DP=27;I16=17,10,0,0,1049,41413,0,0,1615,96625,0,0,558,13090,0,0;QS=3,0;MQSB=0.968979;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,149:5:0 0,24,222:8:0 17 3676 . T 0 . DP=27;I16=17,10,0,0,1040,40640,0,0,1615,96625,0,0,559,13125,0,0;QS=3,0;MQSB=0.968979;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,159:5:0 0,24,231:8:0 17 3677 . A 0 . DP=27;I16=16,10,0,0,922,34182,0,0,1555,93025,0,0,537,12557,0,0;QS=3,0;MQSB=0.965874;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,136:5:0 0,24,217:8:0 17 3678 . G 0 . DP=27;I16=17,10,0,0,1009,38307,0,0,1615,96625,0,0,563,13159,0,0;QS=3,0;MQSB=0.968979;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,149:5:0 0,24,216:8:0 17 3679 . T 0 . DP=27;I16=17,10,0,0,1018,39274,0,0,1615,96625,0,0,563,13105,0,0;QS=3,0;MQSB=0.968979;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,156:5:0 0,24,227:8:0 17 3680 . T 0 . DP=27;I16=17,10,0,0,988,36872,0,0,1615,96625,0,0,562,13022,0,0;QS=3,0;MQSB=0.968979;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,157:5:0 0,24,202:8:0 17 3681 . C 0 . DP=27;I16=17,10,0,0,1012,39154,0,0,1615,96625,0,0,560,12910,0,0;QS=3,0;MQSB=0.968979;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,157:5:0 0,24,217:8:0 17 3682 . A 0 . DP=27;I16=17,10,0,0,1009,38221,0,0,1615,96625,0,0,557,12769,0,0;QS=3,0;MQSB=0.968979;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,156:5:0 0,24,220:8:0 17 3683 . A 0 . DP=27;I16=17,9,0,0,966,36748,0,0,1555,93025,0,0,546,12552,0,0;QS=3,0;MQSB=0.971017;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,153:5:0 0,24,225:8:0 17 3684 . A 0 . DP=26;I16=16,10,0,0,974,37440,0,0,1555,93025,0,0,550,12456,0,0;QS=3,0;MQSB=0.965874;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,155:5:0 0,21,215:7:0 17 3685 . T 0 . DP=26;I16=16,10,0,0,954,36330,0,0,1555,93025,0,0,547,12333,0,0;QS=3,0;MQSB=0.965874;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,148:5:0 0,21,207:7:0 17 3686 . T 0 . DP=26;I16=16,10,0,0,979,37645,0,0,1555,93025,0,0,544,12232,0,0;QS=3,0;MQSB=0.965874;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,162:5:0 0,21,216:7:0 17 3687 . T 0 . DP=27;I16=16,11,0,0,1028,39276,0,0,1592,94394,0,0,541,12153,0,0;QS=3,0;MQSB=0.989102;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,159:5:0 0,24,229:8:0 17 3688 . A 0 . DP=28;I16=17,10,0,0,1007,37883,0,0,1569,92163,0,0,526,11904,0,0;QS=3,0;MQSB=0.99874;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,148:5:0 0,24,232:8:0 17 3689 . T 0 . DP=28;I16=17,11,0,0,1042,39780,0,0,1629,95763,0,0,535,11919,0,0;QS=3,0;MQSB=0.995584;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,162:5:0 0,24,233:8:0 17 3690 . T 0 . DP=28;I16=17,11,0,0,1051,39981,0,0,1629,95763,0,0,532,11816,0,0;QS=3,0;MQSB=0.995584;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,160:5:0 0,24,225:8:0 17 3691 . C 0 . DP=28;I16=16,11,0,0,1037,40549,0,0,1569,92163,0,0,520,11592,0,0;QS=3,0;MQSB=0.992419;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,155:5:0 0,21,214:7:0 17 3692 . A 0 . DP=29;I16=18,11,0,0,1028,37574,0,0,1689,99363,0,0,522,11496,0,0;QS=3,0;MQSB=0.99773;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,156:5:0 0,24,209:8:0 17 3693 . T 0 . DP=28;I16=18,10,0,0,1067,40901,0,0,1629,95763,0,0,519,11381,0,0;QS=3,0;MQSB=0.999713;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,160:5:0 0,24,230:8:0 17 3694 . T 0 . DP=28;I16=18,10,0,0,1043,39659,0,0,1629,95763,0,0,516,11296,0,0;QS=3,0;MQSB=0.999713;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,159:5:0 0,24,234:8:0 17 3695 . T 0 . DP=28;I16=18,10,0,0,1046,39662,0,0,1629,95763,0,0,513,11241,0,0;QS=3,0;MQSB=0.999713;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,156:5:0 0,24,233:8:0 17 3696 . T 0 . DP=28;I16=18,10,0,0,1075,41567,0,0,1629,95763,0,0,509,11165,0,0;QS=3,0;MQSB=0.999713;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,162:5:0 0,24,235:8:0 17 3697 . T 0 . DP=28;I16=18,10,0,0,1031,38399,0,0,1629,95763,0,0,505,11117,0,0;QS=3,0;MQSB=0.999713;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,155:5:0 0,24,229:8:0 17 3698 . A 0 . DP=28;I16=18,9,0,0,983,36457,0,0,1569,92163,0,0,477,10515,0,0;QS=3,0;MQSB=0.999669;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,144:5:0 0,24,229:8:0 17 3699 . A 0 . DP=27;I16=17,10,0,0,1011,38455,0,0,1569,92163,0,0,493,10861,0,0;QS=3,0;MQSB=0.99874;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,150:5:0 0,21,226:7:0 17 3700 . C 0 . DP=28;I16=16,11,0,0,970,35910,0,0,1574,92738,0,0,473,10525,0,0;QS=3,0;MQSB=0.992419;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,168:6:0 0,24,212:8:0 17 3701 . T 0 . DP=28;I16=17,11,0,0,1071,41669,0,0,1634,96338,0,0,484,10618,0,0;QS=3,0;MQSB=0.990092;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,196:6:0 0,24,233:8:0 17 3702 . T 0 . DP=28;I16=17,10,0,0,1019,38653,0,0,1597,94969,0,0,462,10144,0,0;QS=3,0;MQSB=0.968979;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,184:6:0 0,21,207:7:0 17 3703 . C 0 . DP=28;I16=17,11,0,0,1059,40561,0,0,1634,96338,0,0,470,10154,0,0;QS=3,0;MQSB=0.990092;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,183:6:0 0,24,232:8:0 17 3704 . A 0 . DP=28;I16=17,10,0,0,1015,38483,0,0,1574,92738,0,0,439,9347,0,0;QS=3,0;MQSB=0.984677;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,182:6:0 0,21,211:7:0 17 3705 . T 0 . DP=27;I16=17,10,0,0,992,37112,0,0,1574,92738,0,0,459,9773,0,0;QS=3,0;MQSB=0.984677;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,180:6:0 0,21,209:7:0 17 3706 . A 0 . DP=27;I16=17,10,0,0,1040,40262,0,0,1574,92738,0,0,454,9608,0,0;QS=3,0;MQSB=0.984677;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,186:6:0 0,21,204:7:0 17 3707 . G 0 . DP=26;I16=17,9,0,0,974,37394,0,0,1514,89138,0,0,450,9476,0,0;QS=3,0;MQSB=0.977029;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,173:6:0 0,18,168:6:0 17 3708 . T 0 . DP=26;I16=18,7,0,0,911,33609,0,0,1454,85538,0,0,426,8934,0,0;QS=3,0;MQSB=0.914166;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,182:6:0 0,15,139:5:0 17 3709 . A 0 . DP=26;I16=18,8,0,0,905,32473,0,0,1491,86907,0,0,445,9305,0,0;QS=3,0;MQSB=0.998458;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,168:6:0 0,18,162:6:0 17 3710 . C 0 . DP=26;I16=18,8,0,0,924,33504,0,0,1491,86907,0,0,443,9267,0,0;QS=3,0;MQSB=0.998458;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,165:6:0 0,18,159:6:0 17 3711 . C 0 . DP=26;I16=18,8,0,0,953,35965,0,0,1491,86907,0,0,441,9261,0,0;QS=3,0;MQSB=0.998458;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,164:6:0 0,18,173:6:0 17 3712 . A 0 . DP=26;I16=18,8,0,0,893,31917,0,0,1491,86907,0,0,439,9287,0,0;QS=3,0;MQSB=0.998458;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,144:6:0 0,18,165:6:0 17 3713 . C 0 . DP=26;I16=19,7,0,0,908,32834,0,0,1491,86907,0,0,437,9293,0,0;QS=3,0;MQSB=0.989612;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,163:6:0 0,18,164:6:0 17 3714 . A 0 . DP=27;I16=19,6,0,0,920,34366,0,0,1408,81076,0,0,409,8651,0,0;QS=3,0;MQSB=0.999494;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,177:6:0 0,18,174:6:0 17 3715 . T 0 . DP=28;I16=21,6,0,0,982,36286,0,0,1505,86045,0,0,408,8616,0,0;QS=3,0;MQSB=0.996181;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,180:6:0 0,18,164:6:0 17 3716 . T 0 . DP=26;I16=19,7,0,0,927,33833,0,0,1445,82445,0,0,434,9236,0,0;QS=3,0;MQSB=0.966731;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,165:5:0 0,18,164:6:0 17 3717 . C 0 . DP=26;I16=19,7,0,0,979,37527,0,0,1445,82445,0,0,435,9261,0,0;QS=3,0;MQSB=0.966731;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,165:5:0 0,18,168:6:0 17 3718 . T 0 . DP=26;I16=19,7,0,0,994,39040,0,0,1445,82445,0,0,435,9265,0,0;QS=3,0;MQSB=0.966731;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,169:5:0 0,18,165:6:0 17 3719 . A 0 . DP=26;I16=19,6,0,0,889,32163,0,0,1408,81076,0,0,410,8672,0,0;QS=3,0;MQSB=0.747144;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,148:5:0 0,15,142:5:0 17 3720 . C 0 . DP=26;I16=19,7,0,0,934,34326,0,0,1445,82445,0,0,435,9357,0,0;QS=3,0;MQSB=0.966731;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,148:5:0 0,18,158:6:0 17 3721 . A 0 . DP=27;I16=20,5,0,0,910,33944,0,0,1405,80725,0,0,385,8195,0,0;QS=3,0;MQSB=0.697274;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,153:5:0 0,15,145:5:0 17 3722 . C 0 . DP=27;I16=20,7,0,0,964,35276,0,0,1502,85694,0,0,436,9562,0,0;QS=3,0;MQSB=0.927743;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,151:5:0 0,18,165:6:0 17 3723 . A 0 . DP=25;I16=18,6,0,0,888,33516,0,0,1345,77125,0,0,414,9082,0,0;QS=3,0;MQSB=0.606531;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,134:4:0 0,15,145:5:0 17 3724 . C 0 . DP=25;I16=18,7,0,0,944,35956,0,0,1382,78494,0,0,442,9878,0,0;QS=3,0;MQSB=0.889393;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,137:4:0 0,18,169:6:0 17 3725 . T 0 . DP=25;I16=18,7,0,0,973,38263,0,0,1382,78494,0,0,445,10075,0,0;QS=3,0;MQSB=0.889393;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,138:4:0 0,18,190:6:0 17 3726 . G 0 . DP=24;I16=18,6,0,0,865,32339,0,0,1322,74894,0,0,446,10146,0,0;QS=3,0;MQSB=0.934987;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,93:3:0 0,18,166:6:0 17 3727 . C 0 . DP=22;I16=17,5,0,0,762,27812,0,0,1202,67694,0,0,447,10139,0,0;QS=3,0;MQSB=0.963102;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,82:3:0 0,15,143:5:0 17 3728 . C 0 . DP=22;I16=17,5,0,0,792,29850,0,0,1202,67694,0,0,448,10154,0,0;QS=3,0;MQSB=0.963102;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,89:3:0 0,15,160:5:0 17 3729 . C 0 . DP=22;I16=17,5,0,0,838,32312,0,0,1202,67694,0,0,449,10191,0,0;QS=3,0;MQSB=0.963102;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,9,93:3:0 0,15,164:5:0 17 3730 . A 0 . DP=21;I16=17,4,0,0,749,27561,0,0,1142,64094,0,0,448,10100,0,0;QS=3,0;MQSB=0.995997;MQ0F=0 PL:DP:DV 0,39,241:13:0 0,9,98:3:0 0,15,159:5:0 17 3731 . T 0 . DP=22;I16=18,4,0,0,787,29489,0,0,1152,64194,0,0,447,10031,0,0;QS=3,0;MQSB=0.967917;MQ0F=0 PL:DP:DV 0,42,253:14:0 0,9,101:3:0 0,15,139:5:0 17 3732 . G 0 . DP=22;I16=18,4,0,0,811,30695,0,0,1152,64194,0,0,447,9985,0,0;QS=3,0;MQSB=0.967917;MQ0F=0 PL:DP:DV 0,42,252:14:0 0,9,101:3:0 0,15,149:5:0 17 3733 . T 0 . DP=23;I16=19,4,0,0,820,29616,0,0,1212,67794,0,0,447,9963,0,0;QS=3,0;MQSB=0.979651;MQ0F=0 PL:DP:DV 0,45,248:15:0 0,9,101:3:0 0,15,152:5:0 17 3734 . C 0 . DP=24;I16=20,4,0,0,863,32277,0,0,1272,71394,0,0,447,9915,0,0;QS=3,0;MQSB=0.988072;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,86:3:0 0,15,152:5:0 17 3735 . C 0 . DP=24;I16=20,4,0,0,826,30112,0,0,1272,71394,0,0,448,9892,0,0;QS=3,0;MQSB=0.988072;MQ0F=0 PL:DP:DV 0,48,254:16:0 0,9,83:3:0 0,15,152:5:0 17 3736 . C 0 . DP=25;I16=20,3,0,0,839,31471,0,0,1262,71294,0,0,419,9245,0,0;QS=3,0;MQSB=0.963194;MQ0F=0 PL:DP:DV 0,42,235:14:0 0,9,99:3:0 0,18,156:6:0 17 3737 . C 0 . DP=25;I16=21,4,0,0,894,33402,0,0,1332,74994,0,0,451,9925,0,0;QS=3,0;MQSB=0.993838;MQ0F=0 PL:DP:DV 0,48,251:16:0 0,9,95:3:0 0,18,175:6:0 17 3738 . T 0 . DP=25;I16=21,4,0,0,926,35484,0,0,1332,74994,0,0,452,9934,0,0;QS=3,0;MQSB=0.993838;MQ0F=0 PL:DP:DV 0,48,253:16:0 0,9,101:3:0 0,18,189:6:0 17 3739 . C 0 . DP=25;I16=21,4,0,0,958,37390,0,0,1332,74994,0,0,452,9922,0,0;QS=3,0;MQSB=0.993838;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,105:3:0 0,18,181:6:0 17 3740 . A 0 . DP=24;I16=20,4,0,0,848,31126,0,0,1272,71394,0,0,452,9886,0,0;QS=3,0;MQSB=0.988072;MQ0F=0 PL:DP:DV 0,48,247:16:0 0,9,109:3:0 0,15,163:5:0 17 3741 . A 0 . DP=24;I16=19,4,0,0,814,30176,0,0,1212,67794,0,0,442,9742,0,0;QS=3,0;MQSB=0.979651;MQ0F=0 PL:DP:DV 0,48,238:16:0 0,6,82:2:0 0,15,172:5:0 17 3742 . G 0 . DP=24;I16=20,4,0,0,893,34371,0,0,1272,71394,0,0,450,9782,0,0;QS=3,0;MQSB=0.988072;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,101:3:0 0,15,162:5:0 17 3743 . C 0 . DP=24;I16=19,4,0,0,839,31673,0,0,1262,71294,0,0,437,9619,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,254:15:0 0,9,103:3:0 0,15,158:5:0 17 3744 . T 0 . DP=24;I16=20,4,0,0,918,36126,0,0,1272,71394,0,0,448,9766,0,0;QS=3,0;MQSB=0.988072;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,118:3:0 0,15,164:5:0 17 3745 . T 0 . DP=24;I16=19,4,0,0,808,29490,0,0,1212,67794,0,0,422,9166,0,0;QS=3,0;MQSB=0.979651;MQ0F=0 PL:DP:DV 0,45,233:15:0 0,9,108:3:0 0,15,158:5:0 17 3746 . C 0 . DP=24;I16=20,4,0,0,886,33564,0,0,1272,71394,0,0,445,9789,0,0;QS=3,0;MQSB=0.988072;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,9,101:3:0 0,15,165:5:0 17 3747 . C 0 . DP=24;I16=19,4,0,0,833,31071,0,0,1262,71294,0,0,426,9504,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,249:15:0 0,9,103:3:0 0,15,162:5:0 17 3748 . C 0 . DP=24;I16=19,4,0,0,845,31753,0,0,1262,71294,0,0,422,9464,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,9,106:3:0 0,15,161:5:0 17 3749 . C 0 . DP=24;I16=19,4,0,0,844,31888,0,0,1262,71294,0,0,417,9395,0,0;QS=3,0;MQSB=1;MQ0F=0 PL:DP:DV 0,45,250:15:0 0,9,111:3:0 0,15,154:5:0 17 3750 . T 0 . DP=25;I16=21,4,0,0,876,32880,0,0,1309,72763,0,0,431,9709,0,0;QS=3,0;MQSB=0.966906;MQ0F=0 PL:DP:DV 0,51,251:17:0 0,9,112:3:0 0,15,146:5:0 17 3751 . G 0 . DP=24;I16=19,4,0,0,836,31374,0,0,1239,69063,0,0,408,9274,0,0;QS=3,0;MQSB=0.986928;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,82:2:0 0,15,162:5:0 17 3752 . G 0 . DP=22;I16=19,2,0,0,728,26270,0,0,1069,58363,0,0,404,9134,0,0;QS=3,0;MQSB=0.868421;MQ0F=0 PL:DP:DV 0,42,182:14:0 0,6,74:2:0 0,15,159:5:0 17 3753 . C 0 . DP=22;I16=19,3,0,0,761,28017,0,0,1129,61963,0,0,426,9674,0,0;QS=3,0;MQSB=0.995434;MQ0F=0 PL:DP:DV 0,45,213:15:0 0,6,61:2:0 0,15,163:5:0 17 3754 . T 0 . DP=23;I16=19,3,0,0,767,28751,0,0,1129,61963,0,0,425,9707,0,0;QS=3,0;MQSB=0.995434;MQ0F=0 PL:DP:DV 0,45,218:15:0 0,6,69:2:0 0,15,157:5:0 17 3755 . C 0 . DP=21;I16=16,4,0,0,749,28747,0,0,1028,55504,0,0,404,9188,0,0;QS=3,0;MQSB=0.777932;MQ0F=0 PL:DP:DV 0,36,219:12:0 0,9,93:3:0 0,15,161:5:0 17 3756 . C 0 . DP=22;I16=17,4,0,0,782,29994,0,0,1038,55604,0,0,430,9840,0,0;QS=3,0;MQSB=0.885747;MQ0F=0 PL:DP:DV 0,39,229:13:0 0,9,93:3:0 0,15,159:5:0 17 3757 . T 0 . DP=23;I16=17,6,0,0,828,30942,0,0,1158,62804,0,0,456,10510,0,0;QS=3,0;MQSB=0.9945;MQ0F=0 PL:DP:DV 0,39,210:13:0 0,15,143:5:0 0,15,169:5:0 17 3758 . G 0 . DP=23;I16=17,6,0,0,832,30792,0,0,1158,62804,0,0,458,10574,0,0;QS=3,0;MQSB=0.9945;MQ0F=0 PL:DP:DV 0,39,220:13:0 0,15,135:5:0 0,15,147:5:0 17 3759 . C 0 . DP=23;I16=17,4,0,0,756,28338,0,0,1061,57835,0,0,409,9357,0,0;QS=3,0;MQSB=0.964547;MQ0F=0 PL:DP:DV 0,39,220:13:0 0,12,112:4:0 0,12,127:4:0 17 3760 . A 0 . DP=24;I16=17,7,0,0,792,27956,0,0,1218,66404,0,0,459,10607,0,0;QS=3,0;MQSB=0.95083;MQ0F=0 PL:DP:DV 0,39,203:13:0 0,15,127:5:0 0,18,180:6:0 17 3761 . A 0 . DP=24;I16=17,7,0,0,871,32185,0,0,1218,66404,0,0,460,10624,0,0;QS=3,0;MQSB=0.95083;MQ0F=0 PL:DP:DV 0,39,215:13:0 0,15,136:5:0 0,18,188:6:0 17 3762 . C 0 . DP=25;I16=17,7,0,0,831,29665,0,0,1241,68635,0,0,435,9983,0,0;QS=3,0;MQSB=0.69242;MQ0F=0 PL:DP:DV 0,39,209:13:0 0,18,149:6:0 0,15,159:5:0 17 3763 . C 0 . DP=24;I16=16,8,0,0,837,30619,0,0,1218,66404,0,0,460,10510,0,0;QS=3,0;MQSB=0.844324;MQ0F=0 PL:DP:DV 0,36,213:12:0 0,18,149:6:0 0,18,186:6:0 17 3764 . A 0 . DP=25;I16=17,7,0,0,856,31506,0,0,1241,68635,0,0,437,9903,0,0;QS=3,0;MQSB=0.69242;MQ0F=0 PL:DP:DV 0,39,212:13:0 0,18,139:6:0 0,15,167:5:0 17 3765 . C 0 . DP=25;I16=17,7,0,0,845,31099,0,0,1218,66404,0,0,436,9750,0,0;QS=3,0;MQSB=0.95083;MQ0F=0 PL:DP:DV 0,39,200:13:0 0,15,129:5:0 0,18,189:6:0 17 3766 . A 0 . DP=26;I16=17,9,0,0,953,35331,0,0,1338,73604,0,0,462,10340,0,0;QS=3,0;MQSB=0.811273;MQ0F=0 PL:DP:DV 0,42,248:14:0 0,18,158:6:0 0,18,191:6:0 17 3767 . A 0 . DP=26;I16=17,9,0,0,924,33656,0,0,1338,73604,0,0,464,10328,0,0;QS=3,0;MQSB=0.811273;MQ0F=0 PL:DP:DV 0,42,246:14:0 0,18,148:6:0 0,18,191:6:0 17 3768 . A 0 . DP=26;I16=17,9,0,0,922,33842,0,0,1338,73604,0,0,466,10340,0,0;QS=3,0;MQSB=0.811273;MQ0F=0 PL:DP:DV 0,42,237:14:0 0,18,146:6:0 0,18,195:6:0 17 3769 . T 0 . DP=26;I16=17,8,0,0,891,32735,0,0,1278,70004,0,0,461,10327,0,0;QS=3,0;MQSB=0.884621;MQ0F=0 PL:DP:DV 0,42,246:14:0 0,15,144:5:0 0,18,191:6:0 17 3770 . C 0 . DP=26;I16=17,9,0,0,923,34049,0,0,1338,73604,0,0,470,10436,0,0;QS=3,0;MQSB=0.811273;MQ0F=0 PL:DP:DV 0,42,252:14:0 0,18,142:6:0 0,18,180:6:0 17 3771 . T 0 . DP=25;I16=16,8,0,0,852,31694,0,0,1218,66404,0,0,464,10438,0,0;QS=3,0;MQSB=0.844324;MQ0F=0 PL:DP:DV 0,42,253:14:0 0,15,140:5:0 0,15,157:5:0 17 3772 . A 0 . DP=26;I16=16,10,0,0,836,28434,0,0,1338,73604,0,0,476,10624,0,0;QS=3,0;MQSB=0.685145;MQ0F=0 PL:DP:DV 0,42,236:14:0 0,18,122:6:0 0,18,160:6:0 17 3773 . C 0 . DP=26;I16=16,10,0,0,893,31801,0,0,1338,73604,0,0,480,10752,0,0;QS=3,0;MQSB=0.685145;MQ0F=0 PL:DP:DV 0,42,253:14:0 0,18,140:6:0 0,18,171:6:0 17 3774 . T 0 . DP=25;I16=15,10,0,0,895,33455,0,0,1278,70004,0,0,485,10903,0,0;QS=3,0;MQSB=0.624282;MQ0F=0 PL:DP:DV 0,42,253:14:0 0,18,129:6:0 0,15,178:5:0 17 3775 . C 0 . DP=25;I16=15,9,0,0,855,31825,0,0,1241,68635,0,0,477,10883,0,0;QS=3,0;MQSB=0.439649;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,18,147:6:0 0,12,143:4:0 17 3776 . T 0 . DP=24;I16=15,7,0,0,838,32738,0,0,1152,64194,0,0,457,10375,0,0;QS=3,0;MQSB=0.225079;MQ0F=0 PL:DP:DV 0,39,229:13:0 0,15,149:5:0 0,12,152:4:0 17 3777 . C 0 . DP=24;I16=15,9,0,0,864,32180,0,0,1218,66404,0,0,492,10998,0,0;QS=3,0;MQSB=0.705785;MQ0F=0 PL:DP:DV 0,39,220:13:0 0,18,160:6:0 0,15,168:5:0 17 3778 . T 0 . DP=24;I16=14,9,0,0,859,32957,0,0,1208,66304,0,0,468,10372,0,0;QS=3,0;MQSB=0.836049;MQ0F=0 PL:DP:DV 0,36,227:12:0 0,18,157:6:0 0,15,167:5:0 17 3779 . G 0 . DP=24;I16=15,9,0,0,844,31206,0,0,1218,66404,0,0,493,10971,0,0;QS=3,0;MQSB=0.705785;MQ0F=0 PL:DP:DV 0,39,220:13:0 0,18,152:6:0 0,15,167:5:0 17 3780 . C 0 . DP=25;I16=14,10,0,0,829,29949,0,0,1268,69904,0,0,467,10295,0,0;QS=3,0;MQSB=0.765017;MQ0F=0 PL:DP:DV 0,39,235:13:0 0,18,144:6:0 0,15,169:5:0 17 3781 . C 0 . DP=26;I16=16,10,0,0,939,34725,0,0,1315,71373,0,0,492,10896,0,0;QS=3,0;MQSB=0.541994;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,139:6:0 0,15,175:5:0 17 3782 . T 0 . DP=26;I16=16,10,0,0,989,38031,0,0,1315,71373,0,0,493,10901,0,0;QS=3,0;MQSB=0.541994;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,169:6:0 0,15,176:5:0 17 3783 . C 0 . DP=26;I16=16,10,0,0,941,34801,0,0,1315,71373,0,0,492,10836,0,0;QS=3,0;MQSB=0.541994;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,155:6:0 0,15,162:5:0 17 3784 . T 0 . DP=27;I16=16,11,0,0,1001,38035,0,0,1375,74973,0,0,491,10801,0,0;QS=3,0;MQSB=0.467219;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,155:6:0 0,15,178:5:0 17 3785 . G 0 . DP=28;I16=15,10,0,0,922,36426,0,0,1305,71273,0,0,450,9916,0,0;QS=3,0;MQSB=0.674458;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,125:5:0 0,15,179:5:0 17 3786 . T 0 . DP=28;I16=16,11,0,0,935,35697,0,0,1375,74973,0,0,490,10774,0,0;QS=3,0;MQSB=0.467219;MQ0F=0 PL:DP:DV 0,48,250:16:0 0,18,145:6:0 0,15,186:5:0 17 3787 . G 0 . DP=28;I16=16,11,0,0,963,37951,0,0,1375,74973,0,0,489,10781,0,0;QS=3,0;MQSB=0.467219;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,18,143:6:0 0,15,177:5:0 17 3788 . G 0 . DP=26;I16=14,9,0,0,849,33683,0,0,1184,65386,0,0,459,10075,0,0;QS=3,0;MQSB=0.525788;MQ0F=0 PL:DP:DV 0,39,239:13:0 0,18,144:6:0 0,12,161:4:0 17 3789 . G 0 . DP=26;I16=13,10,0,0,807,31019,0,0,1231,68535,0,0,438,9474,0,0;QS=3,0;MQSB=0.445672;MQ0F=0 PL:DP:DV 0,39,251:13:0 0,18,133:6:0 0,12,161:4:0 17 3790 . T 0 . DP=26;I16=13,10,0,0,829,32293,0,0,1231,68535,0,0,435,9359,0,0;QS=3,0;MQSB=0.445672;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,150:6:0 0,12,159:4:0 17 3791 . T 0 . DP=26;I16=15,10,0,0,887,34725,0,0,1301,72235,0,0,478,10336,0,0;QS=3,0;MQSB=0.382304;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,136:6:0 0,12,159:4:0 17 3792 . G A, 0 . DP=26;I16=14,8,1,0,809,32805,38,1444,1175,66425,37,1369,417,8991,22,484;QS=2.92629,0.0737052,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.195278;BQB=1;MQ0F=0 PL:DP:DV 0,8,238,42,241,255:15:1 0,12,109,12,109,109:4:0 0,12,157,12,157,157:4:0 17 3793 . A 0 . DP=26;I16=15,8,0,0,833,33775,0,0,1212,67794,0,0,435,9363,0,0;QS=3,0;MQSB=0.195278;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,12,93:4:0 0,12,159:4:0 17 3794 . C 0 . DP=26;I16=15,9,0,0,845,33023,0,0,1241,68635,0,0,438,9324,0,0;QS=3,0;MQSB=0.439649;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,15,120:5:0 0,12,157:4:0 17 3795 . C 0 . DP=26;I16=14,9,0,0,865,35649,0,0,1231,68535,0,0,408,8622,0,0;QS=3,0;MQSB=0.563599;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,132:5:0 0,12,161:4:0 17 3796 . T 0 . DP=27;I16=15,11,0,0,964,38998,0,0,1361,75835,0,0,453,9821,0,0;QS=3,0;MQSB=0.334895;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,149:7:0 0,12,164:4:0 17 3797 . A 0 . DP=27;I16=15,10,0,0,848,32392,0,0,1301,72235,0,0,424,9172,0,0;QS=3,0;MQSB=0.382304;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,124:6:0 0,12,159:4:0 17 3798 . T 0 . DP=27;I16=14,11,0,0,921,37161,0,0,1301,72235,0,0,430,9554,0,0;QS=3,0;MQSB=0.283586;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,21,148:7:0 0,12,165:4:0 17 3799 . T 0 . DP=27;I16=15,11,0,0,913,35929,0,0,1361,75835,0,0,439,9729,0,0;QS=3,0;MQSB=0.334895;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,21,150:7:0 0,12,161:4:0 17 3800 . C 0 . DP=26;I16=13,10,0,0,866,36182,0,0,1181,65035,0,0,406,9092,0,0;QS=3,0;MQSB=0.272532;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,104:5:0 0,12,161:4:0 17 3801 . T 0 . DP=23;I16=12,10,0,0,804,33600,0,0,1121,61435,0,0,430,9750,0,0;QS=3,0;MQSB=0.217267;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,99:5:0 0,9,133:3:0 17 3802 . G 0 . DP=22;I16=10,9,0,0,730,30516,0,0,994,54486,0,0,380,8550,0,0;QS=3,0;MQSB=0.472367;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,106:4:0 0,9,130:3:0 17 3803 . G 0 . DP=22;I16=11,9,0,0,703,27393,0,0,1051,57735,0,0,405,9241,0,0;QS=3,0;MQSB=0.372419;MQ0F=0 PL:DP:DV 0,39,249:13:0 0,12,92:4:0 0,9,129:3:0 17 3804 . A 0 . DP=22;I16=11,9,0,0,740,30360,0,0,1051,57735,0,0,404,9274,0,0;QS=3,0;MQSB=0.372419;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,98:4:0 0,9,132:3:0 17 3805 . C 0 . DP=22;I16=12,9,0,0,737,29243,0,0,1061,57835,0,0,428,9950,0,0;QS=3,0;MQSB=0.262932;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,81:4:0 0,9,130:3:0 17 3806 . A 0 . DP=22;I16=12,9,0,0,798,32550,0,0,1061,57835,0,0,426,9968,0,0;QS=3,0;MQSB=0.262932;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,12,105:4:0 0,9,128:3:0 17 3807 . C 0 . DP=22;I16=10,9,0,0,664,25304,0,0,994,54486,0,0,377,8885,0,0;QS=3,0;MQSB=0.472367;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,83:4:0 0,9,115:3:0 17 3808 . G 0 . DP=21;I16=9,9,0,0,653,25297,0,0,957,53117,0,0,375,8873,0,0;QS=3,0;MQSB=0.597145;MQ0F=0 PL:DP:DV 0,33,233:11:0 0,12,109:4:0 0,9,132:3:0 17 3809 . T 0 . DP=22;I16=11,10,0,0,779,32151,0,0,1084,60066,0,0,416,9810,0,0;QS=3,0;MQSB=0.285029;MQ0F=0 PL:DP:DV 0,39,249:13:0 0,12,115:4:0 0,12,161:4:0 17 3810 . C 0 . DP=23;I16=9,11,0,0,811,34815,0,0,1077,60317,0,0,369,8739,0,0;QS=3,0;MQSB=0.499893;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,112:4:0 0,12,153:4:0 17 3811 . A C, 0 . DP=23;I16=9,11,2,0,777,32631,39,785,1077,60317,67,3349,367,8669,42,914;QS=2.94104,0.0589569,0;VDB=0.18;SGB=0.346553;RPB=0.425;MQB=0.25;MQSB=0.246128;BQB=0.025;MQ0F=0 PL:DP:DV 0,15,248,36,254,255:14:2 0,12,116,12,116,116:4:0 0,12,158,12,158,158:4:0 17 3812 . T 0 . DP=23;I16=10,11,0,0,802,32860,0,0,1084,60066,0,0,405,9447,0,0;QS=3,0;MQSB=0.187115;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,114:4:0 0,12,160:4:0 17 3813 . A 0 . DP=22;I16=10,11,0,0,815,35077,0,0,1084,60066,0,0,402,9330,0,0;QS=3,0;MQSB=0.187115;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,119:4:0 0,12,170:4:0 17 3814 . G 0 . DP=21;I16=8,11,0,0,775,33731,0,0,1037,58597,0,0,375,8605,0,0;QS=3,0;MQSB=0.41781;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,113:4:0 0,12,163:4:0 17 3815 . A 0 . DP=20;I16=8,11,0,0,708,29130,0,0,1010,57328,0,0,397,9049,0,0;QS=3,0;MQSB=0.373354;MQ0F=0 PL:DP:DV 0,33,239:11:0 0,12,110:4:0 0,12,162:4:0 17 3816 . A 0 . DP=20;I16=8,11,0,0,729,31027,0,0,1010,57328,0,0,395,8933,0,0;QS=3,0;MQSB=0.373354;MQ0F=0 PL:DP:DV 0,33,248:11:0 0,12,104:4:0 0,12,160:4:0 17 3817 . A 0 . DP=20;I16=8,11,0,0,752,31580,0,0,1010,57328,0,0,393,8833,0,0;QS=3,0;MQSB=0.373354;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,12,110:4:0 0,12,161:4:0 17 3818 . T 0 . DP=20;I16=8,11,0,0,728,30466,0,0,1010,57328,0,0,391,8749,0,0;QS=3,0;MQSB=0.373354;MQ0F=0 PL:DP:DV 0,33,252:11:0 0,12,115:4:0 0,12,160:4:0 17 3819 . A 0 . DP=21;I16=9,11,0,0,790,34094,0,0,1039,58169,0,0,389,8681,0,0;QS=3,0;MQSB=0.247381;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,121:4:0 0,12,167:4:0 17 3820 . G 0 . DP=21;I16=9,11,0,0,788,33636,0,0,1039,58169,0,0,388,8630,0,0;QS=3,0;MQSB=0.247381;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,112:4:0 0,12,157:4:0 17 3821 . A 0 . DP=22;I16=10,11,0,0,832,35864,0,0,1099,61769,0,0,387,8597,0,0;QS=3,0;MQSB=0.317882;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,115:4:0 0,12,168:4:0 17 3822 . G 0 . DP=22;I16=10,11,0,0,810,33228,0,0,1099,61769,0,0,386,8532,0,0;QS=3,0;MQSB=0.317882;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,103:4:0 0,12,157:4:0 17 3823 . T 0 . DP=22;I16=9,11,0,0,753,30705,0,0,1042,58520,0,0,380,8460,0,0;QS=3,0;MQSB=0.434285;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,12,102:4:0 0,12,163:4:0 17 3824 . C 0 . DP=22;I16=10,11,0,0,802,32368,0,0,1099,61769,0,0,384,8456,0,0;QS=3,0;MQSB=0.317882;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,105:4:0 0,12,159:4:0 17 3825 . C 0 . DP=23;I16=10,11,0,0,813,33955,0,0,1079,59889,0,0,379,8387,0,0;QS=3,0;MQSB=0.317882;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,130:5:0 0,12,157:4:0 17 3826 . T 0 . DP=23;I16=11,11,0,0,827,34611,0,0,1136,63138,0,0,381,8357,0,0;QS=3,0;MQSB=0.232836;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,133:5:0 0,12,155:4:0 17 3827 . G 0 . DP=23;I16=11,10,0,0,820,34130,0,0,1076,59538,0,0,355,7715,0,0;QS=3,0;MQSB=0.269397;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,121:4:0 0,12,162:4:0 17 3828 . C 0 . DP=23;I16=11,10,0,0,805,33147,0,0,1076,59538,0,0,354,7720,0,0;QS=3,0;MQSB=0.269397;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,12,114:4:0 0,12,157:4:0 17 3829 . A 0 . DP=22;I16=9,11,0,0,763,31295,0,0,1069,59789,0,0,369,8241,0,0;QS=3,0;MQSB=0.477679;MQ0F=0 PL:DP:DV 0,33,255:11:0 0,15,132:5:0 0,12,158:4:0 17 3830 . A 0 . DP=23;I16=11,11,0,0,825,32693,0,0,1136,63138,0,0,377,8321,0,0;QS=3,0;MQSB=0.232836;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,15,143:5:0 0,12,150:4:0 17 3831 . C A, 0 . DP=23;I16=10,11,1,0,802,32198,14,196,1126,63038,10,100,370,8294,7,49;QS=2.97758,0.0224215,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.232836;BQB=1;MQ0F=0 PL:DP:DV 0,27,255,36,255,255:13:1 0,15,134,15,134,134:5:0 0,12,149,12,149,149:4:0 17 3832 . A 0 . DP=24;I16=12,11,0,0,836,32436,0,0,1196,66738,0,0,377,8389,0,0;QS=3,0;MQSB=0.291845;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,15,136:5:0 0,12,139:4:0 17 3833 . C 0 . DP=23;I16=10,11,0,0,690,24938,0,0,1076,59538,0,0,377,8409,0,0;QS=3,0;MQSB=0.175325;MQ0F=0 PL:DP:DV 0,36,249:12:0 0,15,113:5:0 0,12,131:4:0 17 3834 . G 0 . DP=22;I16=9,10,0,0,684,26250,0,0,1037,58597,0,0,357,8079,0,0;QS=3,0;MQSB=0.124514;MQ0F=0 PL:DP:DV 0,33,242:11:0 0,12,122:4:0 0,12,156:4:0 17 3835 . T 0 . DP=22;I16=10,11,0,0,773,30373,0,0,1076,59538,0,0,381,8475,0,0;QS=3,0;MQSB=0.175325;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,15,140:5:0 0,12,153:4:0 17 3836 . G C, 0 . DP=24;I16=10,12,1,0,833,33183,14,196,1132,61648,10,100,378,8412,2,4;QS=2.97647,0.0235294,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.251407;BQB=1;MQ0F=0 PL:DP:DV 0,27,255,36,255,255:13:1 0,15,149,15,149,149:5:0 0,15,188,15,188,188:5:0 17 3837 . G 0 . DP=24;I16=8,14,0,0,759,26931,0,0,1135,61999,0,0,399,8955,0,0;QS=3,0;MQSB=0.291667;MQ0F=0 PL:DP:DV 0,33,245:11:0 0,18,149:6:0 0,15,157:5:0 17 3838 . C 0 . DP=24;I16=10,14,0,0,817,28975,0,0,1202,65348,0,0,409,8945,0,0;QS=3,0;MQSB=0.122456;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,143:6:0 0,15,148:5:0 17 3839 . C 0 . DP=23;I16=9,13,0,0,688,22248,0,0,1132,61648,0,0,386,8238,0,0;QS=3,0;MQSB=0.248197;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,123:6:0 0,12,112:4:0 17 3840 . G 0 . DP=23;I16=9,14,0,0,793,27941,0,0,1192,65248,0,0,413,8809,0,0;QS=3,0;MQSB=0.211072;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,160:6:0 0,15,146:5:0 17 3841 . T 0 . DP=23;I16=9,14,0,0,867,33103,0,0,1192,65248,0,0,414,8734,0,0;QS=3,0;MQSB=0.211072;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,167:6:0 0,15,162:5:0 17 3842 . C 0 . DP=23;I16=9,14,0,0,890,34728,0,0,1192,65248,0,0,415,8689,0,0;QS=3,0;MQSB=0.211072;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,167:6:0 0,15,159:5:0 17 3843 . T 0 . DP=24;I16=9,14,0,0,896,35122,0,0,1192,65248,0,0,416,8674,0,0;QS=3,0;MQSB=0.211072;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,164:6:0 0,15,159:5:0 17 3844 . G 0 . DP=26;I16=10,16,0,0,959,35715,0,0,1372,76048,0,0,442,9314,0,0;QS=3,0;MQSB=0.220358;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,177:7:0 0,18,172:6:0 17 3845 . T 0 . DP=26;I16=10,16,0,0,970,36442,0,0,1372,76048,0,0,444,9310,0,0;QS=3,0;MQSB=0.220358;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,186:7:0 0,18,166:6:0 17 3846 . G 0 . DP=27;I16=10,17,0,0,993,37297,0,0,1432,79648,0,0,446,9338,0,0;QS=3,0;MQSB=0.195223;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,175:7:0 0,21,172:7:0 17 3847 . T 0 . DP=27;I16=10,16,0,0,952,35268,0,0,1372,76048,0,0,423,8723,0,0;QS=3,0;MQSB=0.220358;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,18,170:6:0 0,21,189:7:0 17 3848 . C 0 . DP=27;I16=10,17,0,0,1025,39533,0,0,1432,79648,0,0,449,9341,0,0;QS=3,0;MQSB=0.195223;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,21,177:7:0 0,21,188:7:0 17 3849 . T 0 . DP=27;I16=9,17,0,0,987,37685,0,0,1395,78279,0,0,450,9368,0,0;QS=3,0;MQSB=0.282527;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,179:7:0 0,21,190:7:0 17 3850 . G 0 . DP=26;I16=9,17,0,0,957,35751,0,0,1395,78279,0,0,452,9428,0,0;QS=3,0;MQSB=0.282527;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,179:7:0 0,21,182:7:0 17 3851 . G 0 . DP=26;I16=9,17,0,0,965,36475,0,0,1395,78279,0,0,453,9469,0,0;QS=3,0;MQSB=0.282527;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,21,177:7:0 0,21,183:7:0 17 3852 . C 0 . DP=26;I16=9,16,0,0,921,34525,0,0,1335,74679,0,0,444,9440,0,0;QS=3,0;MQSB=0.310905;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,18,163:6:0 0,21,179:7:0 17 3853 . T 0 . DP=28;I16=10,18,0,0,1050,40222,0,0,1484,82720,0,0,455,9641,0,0;QS=3,0;MQSB=0.515783;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,21,185:7:0 0,21,192:7:0 17 3854 . T 0 . DP=28;I16=10,17,0,0,955,34605,0,0,1455,81879,0,0,451,9709,0,0;QS=3,0;MQSB=0.359211;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,171:6:0 0,18,178:6:0 17 3855 . C 0 . DP=29;I16=10,18,0,0,992,36040,0,0,1515,85479,0,0,438,9264,0,0;QS=3,0;MQSB=0.331344;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,139:5:0 0,21,175:7:0 17 3856 . T 0 . DP=30;I16=10,18,0,0,1033,38725,0,0,1546,88238,0,0,464,9982,0,0;QS=3,0;MQSB=0.190183;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,18,163:6:0 0,21,185:7:0 17 3857 . C 0 . DP=30;I16=10,19,0,0,1077,40979,0,0,1575,89079,0,0,447,9505,0,0;QS=3,0;MQSB=0.306875;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,15,154:5:0 0,24,194:8:0 17 3858 . T 0 . DP=31;I16=10,21,0,0,1141,42567,0,0,1695,96279,0,0,477,10255,0,0;QS=3,0;MQSB=0.266219;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,171:6:0 0,24,208:8:0 17 3859 . C 0 . DP=32;I16=10,21,0,0,950,30078,0,0,1695,96279,0,0,484,10416,0,0;QS=3,0;MQSB=0.266219;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,136:6:0 0,24,156:8:0 17 3860 . G 0 . DP=32;I16=10,22,0,0,1070,37794,0,0,1755,99879,0,0,516,11240,0,0;QS=3,0;MQSB=0.249261;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,154:6:0 0,27,209:9:0 17 3861 . C 0 . DP=33;I16=11,20,0,0,1133,42547,0,0,1672,94048,0,0,517,11391,0,0;QS=3,0;MQSB=0.19205;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,162:6:0 0,24,199:8:0 17 3862 . T 0 . DP=34;I16=11,22,0,0,1241,47443,0,0,1792,101248,0,0,526,11518,0,0;QS=3,0;MQSB=0.161533;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,185:7:0 0,27,221:9:0 17 3863 . T 0 . DP=34;I16=12,22,0,0,1200,43542,0,0,1852,104848,0,0,541,11685,0,0;QS=3,0;MQSB=0.210327;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,199:7:0 0,27,213:9:0 17 3864 . A 0 . DP=33;I16=11,22,0,0,1238,47448,0,0,1792,101248,0,0,550,11790,0,0;QS=3,0;MQSB=0.161533;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,210:7:0 0,27,230:9:0 17 3865 . G 0 . DP=34;I16=11,23,0,0,1251,47113,0,0,1852,104848,0,0,559,11933,0,0;QS=3,0;MQSB=0.149071;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,21,200:7:0 0,27,212:9:0 17 3866 . C 0 . DP=33;I16=11,21,0,0,1165,43545,0,0,1732,97648,0,0,545,11489,0,0;QS=3,0;MQSB=0.175751;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,189:7:0 0,24,197:8:0 17 3867 . A 0 . DP=33;I16=11,22,0,0,1152,41510,0,0,1792,101248,0,0,580,12284,0,0;QS=3,0;MQSB=0.161533;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,188:7:0 0,27,195:9:0 17 3868 . T 0 . DP=33;I16=11,22,0,0,1255,48141,0,0,1792,101248,0,0,590,12494,0,0;QS=3,0;MQSB=0.161533;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,21,202:7:0 0,27,221:9:0 17 3869 . C 0 . DP=33;I16=11,21,0,0,1169,43987,0,0,1732,97648,0,0,589,12623,0,0;QS=3,0;MQSB=0.175751;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,18,164:6:0 0,27,211:9:0 17 3870 . T 0 . DP=34;I16=11,23,0,0,1262,47808,0,0,1852,104848,0,0,608,12932,0,0;QS=3,0;MQSB=0.149071;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,24,215:8:0 0,27,211:9:0 17 3871 . T 0 . DP=36;I16=11,25,0,0,1341,50655,0,0,1972,112048,0,0,617,13157,0,0;QS=3,0;MQSB=0.128391;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,24,221:8:0 0,27,223:9:0 17 3872 . G 0 . DP=36;I16=11,25,0,0,1287,47095,0,0,1972,112048,0,0,626,13322,0,0;QS=3,0;MQSB=0.128391;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,24,211:8:0 0,27,200:9:0 17 3873 . T 0 . DP=35;I16=10,24,0,0,1242,46680,0,0,1852,104848,0,0,626,13428,0,0;QS=3,0;MQSB=0.0982034;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,21,194:7:0 0,24,212:8:0 17 3874 . T 0 . DP=36;I16=12,24,0,0,1290,47660,0,0,1972,112048,0,0,646,13774,0,0;QS=3,0;MQSB=0.182088;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,24,228:8:0 0,24,204:8:0 17 3875 . T 0 . DP=37;I16=13,24,0,0,1324,48418,0,0,2029,115297,0,0,657,14061,0,0;QS=3,0;MQSB=0.117872;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,24,231:8:0 0,24,197:8:0 17 3876 . C 0 . DP=37;I16=13,22,0,0,1248,45354,0,0,1909,108097,0,0,623,13325,0,0;QS=3,0;MQSB=0.140806;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,21,196:7:0 0,24,180:8:0 17 3877 . C 0 . DP=37;I16=13,24,0,0,1363,51201,0,0,2029,115297,0,0,681,14765,0,0;QS=3,0;MQSB=0.117872;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,24,217:8:0 0,24,196:8:0 17 3878 . A 0 . DP=37;I16=13,23,0,0,1309,48045,0,0,1969,111697,0,0,670,14654,0,0;QS=3,0;MQSB=0.128568;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,24,218:8:0 0,24,188:8:0 17 3879 . A 0 . DP=38;I16=14,24,0,0,1453,56567,0,0,2066,116666,0,0,703,15543,0,0;QS=3,0;MQSB=0.076112;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,24,226:8:0 0,24,206:8:0 17 3880 . G 0 . DP=37;I16=14,22,0,0,1311,48735,0,0,1946,109466,0,0,689,15267,0,0;QS=3,0;MQSB=0.094094;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,24,209:8:0 0,24,183:8:0 17 3881 . G 0 . DP=37;I16=14,21,0,0,1200,42778,0,0,1886,105866,0,0,690,15578,0,0;QS=3,0;MQSB=0.105399;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,18,178:6:0 0,24,174:8:0 17 3882 . T 0 . DP=37;I16=14,21,0,0,1192,42352,0,0,1886,105866,0,0,684,15348,0,0;QS=3,0;MQSB=0.105399;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,21,189:7:0 0,21,178:7:0 17 3883 . C 0 . DP=38;I16=15,22,0,0,1295,46659,0,0,2006,113066,0,0,717,16279,0,0;QS=3,0;MQSB=0.124405;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,24,205:8:0 0,24,171:8:0 17 3884 . C 0 . DP=39;I16=16,23,0,0,1363,49387,0,0,2103,118035,0,0,749,17141,0,0;QS=3,0;MQSB=0.0760633;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,24,202:8:0 0,27,212:9:0 17 3885 . T 0 . DP=40;I16=16,24,0,0,1445,53663,0,0,2163,121635,0,0,754,17262,0,0;QS=3,0;MQSB=0.0679472;MQ0F=0 PL:DP:DV 0,69,255:23:0 0,24,219:8:0 0,27,214:9:0 17 3886 . C 0 . DP=39;I16=16,23,0,0,1370,49762,0,0,2103,118035,0,0,761,17421,0,0;QS=3,0;MQSB=0.0760633;MQ0F=0 PL:DP:DV 0,69,255:23:0 0,24,199:8:0 0,24,191:8:0 17 3887 . C 0 . DP=39;I16=16,23,0,0,1364,49222,0,0,2103,118035,0,0,767,17567,0,0;QS=3,0;MQSB=0.0760633;MQ0F=0 PL:DP:DV 0,69,255:23:0 0,24,194:8:0 0,24,201:8:0 17 3888 . C 0 . DP=39;I16=15,23,0,0,1387,51885,0,0,2043,114435,0,0,747,17073,0,0;QS=3,0;MQSB=0.0555905;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,24,195:8:0 0,24,208:8:0 17 3889 . A 0 . DP=38;I16=15,23,0,0,1364,49918,0,0,2066,116666,0,0,777,17811,0,0;QS=3,0;MQSB=0.112471;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,24,202:8:0 0,24,218:8:0 17 3890 . C 0 . DP=38;I16=14,23,0,0,1362,51064,0,0,2006,113066,0,0,757,17329,0,0;QS=3,0;MQSB=0.0844255;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,24,203:8:0 0,24,216:8:0 17 3891 . A 0 . DP=39;I16=15,24,0,0,1466,56312,0,0,2126,120266,0,0,786,18076,0,0;QS=3,0;MQSB=0.102114;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,24,203:8:0 0,27,239:9:0 17 3892 . G 0 . DP=38;I16=15,23,0,0,1340,48838,0,0,2066,116666,0,0,792,18226,0,0;QS=3,0;MQSB=0.112471;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,24,186:8:0 0,27,208:9:0 17 3893 . T 0 . DP=39;I16=15,24,0,0,1348,48170,0,0,2126,120266,0,0,798,18404,0,0;QS=3,0;MQSB=0.102114;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,24,196:8:0 0,27,212:9:0 17 3894 . G 0 . DP=39;I16=15,23,0,0,1364,49900,0,0,2066,116666,0,0,779,17937,0,0;QS=3,0;MQSB=0.112471;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,24,202:8:0 0,27,208:9:0 17 3895 . T 0 . DP=39;I16=15,24,0,0,1375,49773,0,0,2126,120266,0,0,810,18752,0,0;QS=3,0;MQSB=0.102114;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,24,202:8:0 0,27,225:9:0 17 3896 . A 0 . DP=40;I16=15,24,0,0,1468,57326,0,0,2126,120266,0,0,815,18923,0,0;QS=3,0;MQSB=0.102114;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,24,224:8:0 0,27,233:9:0 17 3897 . G 0 . DP=40;I16=15,24,0,0,1468,56812,0,0,2126,120266,0,0,819,19021,0,0;QS=3,0;MQSB=0.102114;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,21,199:7:0 0,30,234:10:0 17 3898 . C 0 . DP=40;I16=15,23,0,0,1487,59351,0,0,2066,116666,0,0,813,19023,0,0;QS=3,0;MQSB=0.112471;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,18,186:6:0 0,30,238:10:0 17 3899 . A 0 . DP=40;I16=15,24,0,0,1449,55055,0,0,2126,120266,0,0,829,19293,0,0;QS=3,0;MQSB=0.102114;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,21,191:7:0 0,30,231:10:0 17 3900 . T 0 . DP=40;I16=15,24,0,0,1458,55516,0,0,2126,120266,0,0,833,19417,0,0;QS=3,0;MQSB=0.102114;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,21,197:7:0 0,30,240:10:0 17 3901 . G 0 . DP=40;I16=14,22,0,0,1303,48245,0,0,1969,111697,0,0,761,17639,0,0;QS=3,0;MQSB=0.180757;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,18,154:6:0 0,27,218:9:0 17 3902 . C 0 . DP=40;I16=15,24,0,0,1436,55412,0,0,2126,120266,0,0,837,19537,0,0;QS=3,0;MQSB=0.102114;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,21,166:7:0 0,30,217:10:0 17 3903 . A 0 . DP=42;I16=15,24,0,0,1299,45567,0,0,2089,117417,0,0,814,19008,0,0;QS=3,0;MQSB=0.0901152;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,24,175:8:0 0,30,204:10:0 17 3904 . C 0 . DP=42;I16=15,24,0,0,1439,54545,0,0,2086,117066,0,0,815,19113,0,0;QS=3,0;MQSB=0.0426917;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,24,191:8:0 0,30,244:10:0 17 3905 . C 0 . DP=42;I16=15,25,0,0,1572,63314,0,0,2146,120666,0,0,822,19192,0,0;QS=3,0;MQSB=0.0381122;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,24,212:8:0 0,33,255:11:0 17 3906 . T 0 . DP=42;I16=16,25,0,0,1593,63039,0,0,2206,124266,0,0,846,19758,0,0;QS=3,0;MQSB=0.0536599;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,24,210:8:0 0,33,253:11:0 17 3907 . G 0 . DP=42;I16=16,25,0,0,1558,60192,0,0,2206,124266,0,0,846,19776,0,0;QS=3,0;MQSB=0.0536599;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,24,203:8:0 0,33,249:11:0 17 3908 . C 0 . DP=42;I16=16,25,0,0,1514,58968,0,0,2206,124266,0,0,845,19777,0,0;QS=3,0;MQSB=0.0536599;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,24,193:8:0 0,33,244:11:0 17 3909 . T 0 . DP=43;I16=16,25,0,0,1491,55895,0,0,2201,123691,0,0,835,19697,0,0;QS=3,0;MQSB=0.0757469;MQ0F=0 PL:DP:DV 0,69,255:23:0 0,24,202:8:0 0,30,237:10:0 17 3910 . A 0 . DP=40;I16=16,23,0,0,1432,53926,0,0,2081,116491,0,0,844,19724,0,0;QS=3,0;MQSB=0.0949554;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,21,193:7:0 0,30,224:10:0 17 3911 . C 0 . DP=40;I16=16,23,0,0,1440,55290,0,0,2081,116491,0,0,843,19613,0,0;QS=3,0;MQSB=0.0949554;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,21,184:7:0 0,30,214:10:0 17 3912 . A C, 0 . DP=41;I16=17,22,0,1,1358,49508,16,256,2081,116491,60,3600,830,19358,11,121;QS=2.95181,0.0481928,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.125266;BQB=1;MQ0F=0 PL:DP:DV 0,69,255,69,255,255:23:0 0,21,166,21,166,166:7:0 0,14,202,27,205,208:10:1 17 3913 . C 0 . DP=40;I16=16,23,0,0,1494,59096,0,0,2081,116491,0,0,824,19100,0,0;QS=3,0;MQSB=0.0949554;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,21,183:7:0 0,30,234:10:0 17 3914 . T 0 . DP=42;I16=16,24,0,0,1512,59342,0,0,2141,120091,0,0,823,19007,0,0;QS=3,0;MQSB=0.0846181;MQ0F=0 PL:DP:DV 0,69,255:23:0 0,21,192:7:0 0,30,228:10:0 17 3915 . C 0 . DP=42;I16=16,24,0,0,1537,60953,0,0,2141,120091,0,0,799,18321,0,0;QS=3,0;MQSB=0.0846181;MQ0F=0 PL:DP:DV 0,72,255:24:0 0,18,176:6:0 0,30,229:10:0 17 3916 . C 0 . DP=42;I16=16,25,0,0,1618,66342,0,0,2201,123691,0,0,825,18919,0,0;QS=3,0;MQSB=0.0757469;MQ0F=0 PL:DP:DV 0,72,255:24:0 0,21,190:7:0 0,30,251:10:0 17 3917 . T 0 . DP=43;I16=16,25,0,0,1601,65219,0,0,2201,123691,0,0,825,18875,0,0;QS=3,0;MQSB=0.0757469;MQ0F=0 PL:DP:DV 0,72,255:24:0 0,21,191:7:0 0,30,254:10:0 17 3918 . T 0 . DP=43;I16=16,25,0,0,1541,60711,0,0,2201,123691,0,0,801,18239,0,0;QS=3,0;MQSB=0.0757469;MQ0F=0 PL:DP:DV 0,75,255:25:0 0,18,179:6:0 0,30,254:10:0 17 3919 . C 0 . DP=42;I16=15,26,0,0,1621,66337,0,0,2232,126450,0,0,826,18786,0,0;QS=3,0;MQSB=0.110793;MQ0F=0 PL:DP:DV 0,72,255:24:0 0,21,200:7:0 0,30,242:10:0 17 3920 . T 0 . DP=42;I16=15,26,0,0,1605,64327,0,0,2232,126450,0,0,825,18691,0,0;QS=3,0;MQSB=0.110793;MQ0F=0 PL:DP:DV 0,72,255:24:0 0,21,207:7:0 0,30,251:10:0 17 3921 . T 0 . DP=42;I16=15,26,0,0,1519,58507,0,0,2232,126450,0,0,824,18630,0,0;QS=3,0;MQSB=0.110793;MQ0F=0 PL:DP:DV 0,72,255:24:0 0,21,201:7:0 0,30,242:10:0 17 3922 . A 0 . DP=42;I16=14,26,0,0,1539,61289,0,0,2195,125081,0,0,819,18545,0,0;QS=3,0;MQSB=0.168991;MQ0F=0 PL:DP:DV 0,72,255:24:0 0,18,179:6:0 0,30,244:10:0 17 3923 . G 0 . DP=41;I16=14,25,0,0,1515,60945,0,0,2135,121481,0,0,792,17834,0,0;QS=3,0;MQSB=0.182501;MQ0F=0 PL:DP:DV 0,69,255:23:0 0,18,172:6:0 0,30,240:10:0 17 3924 . G 0 . DP=41;I16=13,26,0,0,1524,61106,0,0,2135,121481,0,0,808,18356,0,0;QS=3,0;MQSB=0.128469;MQ0F=0 PL:DP:DV 0,69,255:23:0 0,18,158:6:0 0,30,247:10:0 17 3925 . G 0 . DP=41;I16=14,25,0,0,1425,55687,0,0,2135,121481,0,0,788,17758,0,0;QS=3,0;MQSB=0.182501;MQ0F=0 PL:DP:DV 0,72,255:24:0 0,15,134:5:0 0,30,245:10:0 17 3926 . C 0 . DP=41;I16=14,26,0,0,1512,59674,0,0,2195,125081,0,0,811,18393,0,0;QS=3,0;MQSB=0.168991;MQ0F=0 PL:DP:DV 0,72,255:24:0 0,18,152:6:0 0,30,237:10:0 17 3927 . T 0 . DP=41;I16=14,26,0,0,1512,59566,0,0,2195,125081,0,0,808,18384,0,0;QS=3,0;MQSB=0.168991;MQ0F=0 PL:DP:DV 0,72,255:24:0 0,18,160:6:0 0,30,237:10:0 17 3928 . G 0 . DP=41;I16=14,25,0,0,1479,58495,0,0,2135,121481,0,0,779,17731,0,0;QS=3,0;MQSB=0.182501;MQ0F=0 PL:DP:DV 0,72,255:24:0 0,18,149:6:0 0,27,233:9:0 17 3929 . A 0 . DP=41;I16=13,26,0,0,1452,56436,0,0,2135,121481,0,0,796,18256,0,0;QS=3,0;MQSB=0.128469;MQ0F=0 PL:DP:DV 0,69,255:23:0 0,18,161:6:0 0,30,220:10:0 17 3930 . T 0 . DP=40;I16=13,25,0,0,1387,52929,0,0,2078,118232,0,0,767,17521,0,0;QS=3,0;MQSB=0.25797;MQ0F=0 PL:DP:DV 0,69,255:23:0 0,15,149:5:0 0,30,224:10:0 17 3931 . A 0 . DP=40;I16=13,25,0,0,1387,52877,0,0,2078,118232,0,0,761,17439,0,0;QS=3,0;MQSB=0.25797;MQ0F=0 PL:DP:DV 0,69,255:23:0 0,18,166:6:0 0,27,222:9:0 17 3932 . T 0 . DP=39;I16=12,26,0,0,1394,53644,0,0,2078,118232,0,0,780,17964,0,0;QS=3,0;MQSB=0.190372;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,18,161:6:0 0,30,231:10:0 17 3933 . T 0 . DP=38;I16=12,24,0,0,1389,54743,0,0,1958,111032,0,0,752,17366,0,0;QS=3,0;MQSB=0.218161;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,18,166:6:0 0,24,220:8:0 17 3934 . C 0 . DP=38;I16=12,25,0,0,1395,54915,0,0,2018,114632,0,0,768,17758,0,0;QS=3,0;MQSB=0.203497;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,18,152:6:0 0,27,209:9:0 17 3935 . C 0 . DP=38;I16=12,24,0,0,1239,44621,0,0,1958,111032,0,0,737,17075,0,0;QS=3,0;MQSB=0.218161;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,15,134:5:0 0,27,170:9:0 17 3936 . A G, 0 . DP=37;I16=5,6,6,17,424,16384,801,30271,609,34563,1314,77018,225,5325,511,11851;QS=0.87994,2.12006,0;VDB=0.0574114;SGB=-4.60123;RPB=0.741697;MQB=0.812605;MQSB=0.143788;BQB=0.883831;MQ0F=0 PL:DP:DV 233,0,206,255,239,255:20:11 77,0,58,83,70,141:6:4 196,24,0,196,24,196:8:8 17 3937 . C 0 . DP=36;I16=11,24,0,0,1155,39875,0,0,1952,112422,0,0,745,17291,0,0;QS=3,0;MQSB=0.219636;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,18,143:6:0 0,24,156:8:0 17 3938 . G 0 . DP=35;I16=11,23,0,0,1155,41761,0,0,1892,108822,0,0,737,17079,0,0;QS=3,0;MQSB=0.231054;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,15,119:5:0 0,24,176:8:0 17 3939 . C 0 . DP=35;I16=11,22,0,0,1273,50651,0,0,1832,105222,0,0,703,16221,0,0;QS=3,0;MQSB=0.243713;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,15,135:5:0 0,21,174:7:0 17 3940 . A 0 . DP=35;I16=11,22,0,0,1148,42754,0,0,1832,105222,0,0,691,15867,0,0;QS=3,0;MQSB=0.243713;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,15,117:5:0 0,21,163:7:0 17 3941 . C 0 . DP=35;I16=11,22,0,0,1216,47488,0,0,1832,105222,0,0,688,15892,0,0;QS=3,0;MQSB=0.243713;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,15,133:5:0 0,21,181:7:0 17 3942 . C 0 . DP=35;I16=11,23,0,0,1336,54166,0,0,1892,108822,0,0,690,15772,0,0;QS=3,0;MQSB=0.231054;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,15,140:5:0 0,24,195:8:0 17 3943 . T 0 . DP=35;I16=11,23,0,0,1296,51618,0,0,1892,108822,0,0,676,15406,0,0;QS=3,0;MQSB=0.231054;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,15,142:5:0 0,24,195:8:0 17 3944 . G 0 . DP=34;I16=10,22,0,0,1199,46445,0,0,1803,104381,0,0,654,14954,0,0;QS=3,0;MQSB=0.1117;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,149:5:0 0,24,195:8:0 17 3945 . C 0 . DP=33;I16=10,22,0,0,1256,51226,0,0,1772,101622,0,0,649,14657,0,0;QS=3,0;MQSB=0.187579;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,15,142:5:0 0,21,177:7:0 17 3946 . T 0 . DP=33;I16=10,21,0,0,1170,45884,0,0,1712,98022,0,0,609,13599,0,0;QS=3,0;MQSB=0.199344;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,15,144:5:0 0,18,158:6:0 17 3947 . A 0 . DP=32;I16=10,21,0,0,1094,41048,0,0,1712,98022,0,0,620,13820,0,0;QS=3,0;MQSB=0.199344;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,15,128:5:0 0,18,149:6:0 17 3948 . C 0 . DP=32;I16=10,20,0,0,1134,45104,0,0,1652,94422,0,0,596,13344,0,0;QS=3,0;MQSB=0.212591;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,12,105:4:0 0,18,155:6:0 17 3949 . A C, 0 . DP=32;I16=10,17,0,1,1027,39909,24,576,1472,83622,60,3600,541,12211,25,625;QS=2.85093,0.149068,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.244621;BQB=1;MQ0F=0 PL:DP:DV 0,60,255,60,255,255:20:0 0,9,89,9,89,89:3:0 9,0,107,21,110,124:5:1 17 3950 . C 0 . DP=33;I16=11,21,0,0,1191,46247,0,0,1772,101622,0,0,576,12680,0,0;QS=3,0;MQSB=0.257801;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,15,118:5:0 0,18,168:6:0 17 3951 . T 0 . DP=34;I16=12,19,0,0,1148,44272,0,0,1712,98022,0,0,530,11670,0,0;QS=3,0;MQSB=0.354733;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,15,146:5:0 0,15,152:5:0 17 3952 . C 0 . DP=35;I16=13,20,0,0,1176,43762,0,0,1832,105222,0,0,545,12025,0,0;QS=3,0;MQSB=0.394875;MQ0F=0 PL:DP:DV 0,66,255:22:0 0,18,158:6:0 0,15,143:5:0 17 3953 . C 0 . DP=34;I16=13,20,0,0,1246,48436,0,0,1863,107981,0,0,538,11772,0,0;QS=3,0;MQSB=0.252983;MQ0F=0 PL:DP:DV 0,63,255:21:0 0,18,163:6:0 0,18,168:6:0 17 3954 . T 0 . DP=33;I16=13,19,0,0,1195,45815,0,0,1803,104381,0,0,526,11438,0,0;QS=3,0;MQSB=0.264585;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,18,171:6:0 0,18,167:6:0 17 3955 . T 0 . DP=32;I16=13,18,0,0,1180,46092,0,0,1743,100781,0,0,515,11139,0,0;QS=3,0;MQSB=0.277468;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,18,173:6:0 0,18,174:6:0 17 3956 . C 0 . DP=32;I16=13,18,0,0,1181,47021,0,0,1743,100781,0,0,504,10874,0,0;QS=3,0;MQSB=0.277468;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,18,167:6:0 0,18,167:6:0 17 3957 . T 0 . DP=31;I16=13,17,0,0,1153,46051,0,0,1683,97181,0,0,494,10642,0,0;QS=3,0;MQSB=0.291833;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,18,161:6:0 0,15,162:5:0 17 3958 . T 0 . DP=31;I16=13,16,0,0,1070,40600,0,0,1623,93581,0,0,483,10393,0,0;QS=3,0;MQSB=0.307929;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,15,139:5:0 0,15,157:5:0 17 3959 . A 0 . DP=30;I16=14,15,0,0,1062,39758,0,0,1623,93581,0,0,474,10176,0,0;QS=3,0;MQSB=0.377103;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,12,118:4:0 0,15,154:5:0 17 3960 . T 0 . DP=30;I16=14,15,0,0,995,36027,0,0,1623,93581,0,0,464,9892,0,0;QS=3,0;MQSB=0.377103;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,12,105:4:0 0,15,142:5:0 17 3961 . G 0 . DP=29;I16=12,16,0,0,1067,40899,0,0,1586,92212,0,0,457,9739,0,0;QS=3,0;MQSB=0.455864;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,12,114:4:0 0,12,122:4:0 17 3962 . G 0 . DP=29;I16=13,16,0,0,1025,37125,0,0,1623,93581,0,0,471,10053,0,0;QS=3,0;MQSB=0.307929;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,12,108:4:0 0,15,144:5:0 17 3963 . C 0 . DP=28;I16=13,15,0,0,1040,39100,0,0,1563,89981,0,0,463,9871,0,0;QS=3,0;MQSB=0.326055;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,12,111:4:0 0,12,126:4:0 17 3964 . T 0 . DP=27;I16=12,15,0,0,1036,39928,0,0,1503,86381,0,0,456,9720,0,0;QS=3,0;MQSB=0.273507;MQ0F=0 PL:DP:DV 0,60,255:20:0 0,9,94:3:0 0,12,133:4:0 17 3965 . G 0 . DP=26;I16=12,14,0,0,993,38165,0,0,1443,82781,0,0,450,9598,0,0;QS=3,0;MQSB=0.29215;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,9,92:3:0 0,12,134:4:0 17 3966 . A 0 . DP=26;I16=12,13,0,0,930,34834,0,0,1406,81412,0,0,432,9310,0,0;QS=3,0;MQSB=0.520812;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,9,90:3:0 0,12,140:4:0 17 3967 . T 0 . DP=26;I16=12,13,0,0,900,32830,0,0,1406,81412,0,0,421,9001,0,0;QS=3,0;MQSB=0.520812;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,9,90:3:0 0,9,100:3:0 17 3968 . A 0 . DP=25;I16=12,13,0,0,885,31773,0,0,1406,81412,0,0,415,8853,0,0;QS=3,0;MQSB=0.520812;MQ0F=0 PL:DP:DV 0,57,255:19:0 0,9,90:3:0 0,9,99:3:0 17 3969 . T 0 . DP=24;I16=11,13,0,0,886,32972,0,0,1369,80043,0,0,410,8736,0,0;QS=3,0;MQSB=0.702671;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,9,85:3:0 0,9,108:3:0 17 3970 . T 0 . DP=24;I16=11,13,0,0,861,31313,0,0,1369,80043,0,0,405,8649,0,0;QS=3,0;MQSB=0.702671;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,9,83:3:0 0,9,104:3:0 17 3971 . C 0 . DP=22;I16=11,11,0,0,815,30625,0,0,1249,72843,0,0,402,8590,0,0;QS=3,0;MQSB=0.751921;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,57:2:0 0,9,103:3:0 17 3972 . C 0 . DP=22;I16=11,11,0,0,812,30486,0,0,1249,72843,0,0,399,8557,0,0;QS=3,0;MQSB=0.751921;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,55:2:0 0,9,96:3:0 17 3973 . A 0 . DP=22;I16=11,11,0,0,795,28873,0,0,1249,72843,0,0,395,8501,0,0;QS=3,0;MQSB=0.751921;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,52:2:0 0,9,97:3:0 17 3974 . C 0 . DP=22;I16=11,11,0,0,729,24447,0,0,1249,72843,0,0,392,8472,0,0;QS=3,0;MQSB=0.751921;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,55:2:0 0,9,78:3:0 17 3975 . G 0 . DP=22;I16=11,11,0,0,717,24525,0,0,1249,72843,0,0,390,8470,0,0;QS=3,0;MQSB=0.751921;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,46:2:0 0,9,96:3:0 17 3976 . C 0 . DP=22;I16=11,11,0,0,816,30652,0,0,1249,72843,0,0,387,8445,0,0;QS=3,0;MQSB=0.751921;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,55:2:0 0,9,97:3:0 17 3977 . A 0 . DP=23;I16=11,11,0,0,740,25384,0,0,1249,72843,0,0,382,8346,0,0;QS=3,0;MQSB=0.751921;MQ0F=0 PL:DP:DV 0,51,255:17:0 0,6,48:2:0 0,9,90:3:0 17 3978 . C 0 . DP=23;I16=11,12,0,0,769,26631,0,0,1309,76443,0,0,377,8223,0,0;QS=3,0;MQSB=0.726094;MQ0F=0 PL:DP:DV 0,54,255:18:0 0,6,47:2:0 0,9,103:3:0 17 3979 . C 0 . DP=21;I16=9,11,0,0,744,28160,0,0,1152,67874,0,0,361,7905,0,0;QS=3,0;MQSB=0.885207;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,51:2:0 0,9,104:3:0 17 3980 . T 0 . DP=21;I16=10,11,0,0,756,27872,0,0,1212,71474,0,0,367,7855,0,0;QS=3,0;MQSB=0.914611;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,51:2:0 0,9,98:3:0 17 3981 . G 0 . DP=21;I16=10,11,0,0,785,29641,0,0,1212,71474,0,0,362,7710,0,0;QS=3,0;MQSB=0.914611;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,50:2:0 0,9,93:3:0 17 3982 . C 0 . DP=21;I16=10,11,0,0,779,29495,0,0,1212,71474,0,0,357,7591,0,0;QS=3,0;MQSB=0.914611;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,55:2:0 0,9,97:3:0 17 3983 . T 0 . DP=20;I16=9,11,0,0,720,26274,0,0,1155,68225,0,0,353,7497,0,0;QS=3,0;MQSB=0.993528;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,57:2:0 0,9,93:3:0 17 3984 . A 0 . DP=21;I16=10,11,0,0,739,26279,0,0,1192,69594,0,0,348,7378,0,0;QS=3,0;MQSB=0.885602;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,53:2:0 0,9,80:3:0 17 3985 . C 0 . DP=20;I16=10,10,0,0,728,26998,0,0,1132,65994,0,0,344,7234,0,0;QS=3,0;MQSB=0.902256;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,55:2:0 0,9,91:3:0 17 3986 . A 0 . DP=20;I16=10,10,0,0,685,23815,0,0,1132,65994,0,0,340,7114,0,0;QS=3,0;MQSB=0.902256;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,56:2:0 0,9,84:3:0 17 3987 . C 0 . DP=20;I16=10,10,0,0,736,28118,0,0,1132,65994,0,0,336,7018,0,0;QS=3,0;MQSB=0.902256;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,55:2:0 0,9,94:3:0 17 3988 . T 0 . DP=21;I16=10,10,0,0,741,27797,0,0,1132,65994,0,0,332,6946,0,0;QS=3,0;MQSB=0.902256;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,58:2:0 0,9,91:3:0 17 3989 . C 0 . DP=21;I16=10,11,0,0,729,26185,0,0,1192,69594,0,0,327,6801,0,0;QS=3,0;MQSB=0.885602;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,55:2:0 0,9,88:3:0 17 3990 . C 0 . DP=21;I16=10,11,0,0,766,28674,0,0,1192,69594,0,0,322,6686,0,0;QS=3,0;MQSB=0.885602;MQ0F=0 PL:DP:DV 0,48,255:16:0 0,6,55:2:0 0,9,95:3:0 17 3991 . T 0 . DP=20;I16=9,11,0,0,729,27311,0,0,1132,65994,0,0,318,6600,0,0;QS=3,0;MQSB=0.850154;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,55:2:0 0,9,89:3:0 17 3992 . T 0 . DP=19;I16=9,10,0,0,667,24173,0,0,1072,62394,0,0,313,6441,0,0;QS=3,0;MQSB=0.868634;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,56:2:0 0,6,72:2:0 17 3993 . C 0 . DP=18;I16=9,9,0,0,701,27529,0,0,1012,58794,0,0,309,6307,0,0;QS=3,0;MQSB=0.888755;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,59:2:0 0,6,70:2:0 17 3994 . T 0 . DP=19;I16=10,9,0,0,718,27520,0,0,1049,60163,0,0,305,6197,0,0;QS=3,0;MQSB=0.716531;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,58:2:0 0,6,72:2:0 17 3995 . T 0 . DP=19;I16=9,9,0,0,641,23025,0,0,989,56563,0,0,277,5487,0,0;QS=3,0;MQSB=0.650623;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,53:2:0 0,6,69:2:0 17 3996 . A 0 . DP=19;I16=9,9,0,0,665,24815,0,0,989,56563,0,0,274,5428,0,0;QS=3,0;MQSB=0.650623;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,54:2:0 0,6,73:2:0 17 3997 . G C, 0 . DP=19;I16=10,8,0,1,645,23703,21,441,989,56563,60,3600,287,5843,6,36;QS=2.96023,0.0397727,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.716531;BQB=1;MQ0F=0 PL:DP:DV 0,24,255,42,255,255:15:1 0,6,57,6,57,57:2:0 0,6,60,6,60,60:2:0 17 3998 . G 0 . DP=18;I16=9,8,0,0,626,23710,0,0,929,52963,0,0,265,5203,0,0;QS=3,0;MQSB=0.687289;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,6,58:2:0 0,3,36:1:0 17 3999 . G A, 0 . DP=18;I16=9,7,0,1,596,22974,37,1369,869,49363,60,3600,263,5387,4,16;QS=2.92871,0.0712909,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.687289;BQB=1;MQ0F=0 PL:DP:DV 0,5,255,39,255,255:14:1 0,6,56,6,56,56:2:0 0,3,38,3,38,38:1:0 17 4000 . C 0 . DP=18;I16=10,8,0,0,669,25389,0,0,989,56563,0,0,283,5753,0,0;QS=3,0;MQSB=0.751866;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,55:2:0 0,3,36:1:0 17 4001 . T 0 . DP=18;I16=10,8,0,0,680,26006,0,0,989,56563,0,0,279,5727,0,0;QS=3,0;MQSB=0.751866;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,56:2:0 0,3,33:1:0 17 4002 . G 0 . DP=17;I16=10,7,0,0,621,23281,0,0,929,52963,0,0,276,5724,0,0;QS=2,0;MQSB=0.79189;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,44:2:0 0,0,0:0:0 17 4003 . A 0 . DP=17;I16=10,7,0,0,597,21507,0,0,929,52963,0,0,272,5692,0,0;QS=2,0;MQSB=0.79189;MQ0F=0 PL:DP:DV 0,45,255:15:0 0,6,55:2:0 0,0,0:0:0 17 4004 . T 0 . DP=15;I16=9,6,0,0,527,19031,0,0,849,48963,0,0,270,5678,0,0;QS=2,0;MQSB=0.957526;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,3,40:1:0 0,0,0:0:0 17 4005 . A 0 . DP=15;I16=8,5,0,0,479,17731,0,0,729,41763,0,0,237,5195,0,0;QS=2,0;MQSB=0.958048;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,3,35:1:0 0,0,0:0:0 17 4006 . T 0 . DP=15;I16=9,6,0,0,554,20776,0,0,849,48963,0,0,266,5698,0,0;QS=2,0;MQSB=0.957526;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,3,40:1:0 0,0,0:0:0 17 4007 . T G, 0 . DP=15;I16=9,5,0,1,490,17810,15,225,789,45363,60,3600,245,5371,19,361;QS=1.96746,0.032538,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.957526;BQB=1;MQ0F=0 PL:DP:DV 0,26,255,39,255,255:14:1 0,3,41,3,41,41:1:0 0,0,0,0,0,0:0:0 17 4008 . C 0 . DP=15;I16=9,6,0,0,488,16890,0,0,849,48963,0,0,262,5782,0,0;QS=2,0;MQSB=0.957526;MQ0F=0 PL:DP:DV 0,42,255:14:0 0,3,30:1:0 0,0,0:0:0 17 4009 . C 0 . DP=14;I16=9,5,0,0,524,20150,0,0,794,45938,0,0,261,5847,0,0;QS=2,0;MQSB=0.800737;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,3,40:1:0 0,0,0:0:0 17 4010 . A 0 . DP=14;I16=9,5,0,0,523,19731,0,0,794,45938,0,0,259,5875,0,0;QS=2,0;MQSB=0.800737;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,3,41:1:0 0,0,0:0:0 17 4011 . C 0 . DP=14;I16=9,5,0,0,489,17613,0,0,794,45938,0,0,257,5915,0,0;QS=2,0;MQSB=0.800737;MQ0F=0 PL:DP:DV 0,39,255:13:0 0,3,41:1:0 0,0,0:0:0 17 4012 . A 0 . DP=14;I16=7,5,0,0,365,11867,0,0,674,38738,0,0,223,5293,0,0;QS=2,0;MQSB=0.6821;MQ0F=0 PL:DP:DV 0,33,231:11:0 0,3,30:1:0 0,0,0:0:0 17 4013 . C 0 . DP=14;I16=8,5,0,0,451,15885,0,0,734,42338,0,0,247,5995,0,0;QS=2,0;MQSB=0.765017;MQ0F=0 PL:DP:DV 0,36,255:12:0 0,3,37:1:0 0,0,0:0:0 17 4014 . A 0 . DP=12;I16=8,2,0,0,358,13372,0,0,554,31538,0,0,222,5404,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,213:9:0 0,3,34:1:0 0,0,0:0:0 17 4015 . C 0 . DP=12;I16=8,2,0,0,350,12812,0,0,554,31538,0,0,222,5442,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,222:9:0 0,3,21:1:0 0,0,0:0:0 17 4016 . C 0 . DP=12;I16=8,3,0,0,350,11956,0,0,614,35138,0,0,247,6109,0,0;QS=2,0;MQSB=0.829029;MQ0F=0 PL:DP:DV 0,30,215:10:0 0,3,32:1:0 0,0,0:0:0 17 4017 . C 0 . DP=11;I16=5,2,0,0,227,7765,0,0,374,20738,0,0,173,4279,0,0;QS=2,0;MQSB=0.6;MQ0F=0 PL:DP:DV 0,18,161:6:0 0,3,20:1:0 0,0,0:0:0 17 4018 . G 0 . DP=11;I16=8,2,0,0,284,8442,0,0,554,31538,0,0,249,6201,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,183:9:0 0,3,17:1:0 0,0,0:0:0 17 4019 . C 0 . DP=11;I16=9,2,0,0,383,14427,0,0,614,35138,0,0,250,6250,0,0;QS=2,0;MQSB=0.777778;MQ0F=0 PL:DP:DV 0,30,228:10:0 0,3,33:1:0 0,0,0:0:0 17 4020 . T 0 . DP=10;I16=8,2,0,0,362,13668,0,0,554,31538,0,0,250,6250,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,213:9:0 0,3,38:1:0 0,0,0:0:0 17 4021 . A 0 . DP=10;I16=8,1,0,0,304,10512,0,0,494,27938,0,0,225,5625,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,177:8:0 0,3,33:1:0 0,0,0:0:0 17 4022 . C 0 . DP=10;I16=7,2,0,0,310,11582,0,0,494,27938,0,0,225,5625,0,0;QS=2,0;MQSB=0.714286;MQ0F=0 PL:DP:DV 0,24,202:8:0 0,3,36:1:0 0,0,0:0:0 17 4023 . A 0 . DP=10;I16=8,2,0,0,354,13116,0,0,554,31538,0,0,250,6250,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,209:9:0 0,3,40:1:0 0,0,0:0:0 17 4024 . C 0 . DP=10;I16=8,2,0,0,361,13669,0,0,554,31538,0,0,250,6250,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,214:9:0 0,3,39:1:0 0,0,0:0:0 17 4025 . T 0 . DP=10;I16=8,1,0,0,360,14488,0,0,494,27938,0,0,224,5576,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,199:8:0 0,3,42:1:0 0,0,0:0:0 17 4026 . C 0 . DP=10;I16=8,2,0,0,329,11655,0,0,554,31538,0,0,248,6154,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,192:9:0 0,3,39:1:0 0,0,0:0:0 17 4027 . C 0 . DP=10;I16=8,2,0,0,380,14888,0,0,554,31538,0,0,246,6060,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,231:9:0 0,3,38:1:0 0,0,0:0:0 17 4028 . T 0 . DP=10;I16=8,2,0,0,333,12157,0,0,554,31538,0,0,244,5970,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,202:9:0 0,3,41:1:0 0,0,0:0:0 17 4029 . T 0 . DP=10;I16=8,2,0,0,377,14333,0,0,554,31538,0,0,242,5884,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,225:9:0 0,3,38:1:0 0,0,0:0:0 17 4030 . C 0 . DP=10;I16=8,2,0,0,348,12558,0,0,554,31538,0,0,240,5802,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,211:9:0 0,3,30:1:0 0,0,0:0:0 17 4031 . T 0 . DP=10;I16=8,2,0,0,388,15302,0,0,554,31538,0,0,238,5724,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,229:9:0 0,3,39:1:0 0,0,0:0:0 17 4032 . T 0 . DP=10;I16=8,2,0,0,341,11901,0,0,554,31538,0,0,236,5650,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,204:9:0 0,3,39:1:0 0,0,0:0:0 17 4033 . A 0 . DP=10;I16=7,2,0,0,299,10717,0,0,494,27938,0,0,218,5324,0,0;QS=2,0;MQSB=0.714286;MQ0F=0 PL:DP:DV 0,24,193:8:0 0,3,38:1:0 0,0,0:0:0 17 4034 . G 0 . DP=10;I16=8,2,0,0,347,12527,0,0,554,31538,0,0,231,5465,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,211:9:0 0,3,39:1:0 0,0,0:0:0 17 4035 . G T, 0 . DP=10;I16=7,2,1,0,316,11900,13,169,494,27938,60,3600,202,4682,25,625;QS=1.9547,0.0452962,0;SGB=-0.556633;RPB=1;MQB=1;MQSB=0.75;BQB=1;MQ0F=0 PL:DP:DV 0,13,195,24,198,200:9:1 0,3,31,3,31,31:1:0 0,0,0,0,0,0:0:0 17 4036 . G 0 . DP=10;I16=8,1,0,0,269,8839,0,0,494,27938,0,0,198,4532,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,156:8:0 0,3,34:1:0 0,0,0:0:0 17 4037 . C 0 . DP=10;I16=8,2,0,0,342,12466,0,0,554,31538,0,0,219,5015,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,210:9:0 0,3,30:1:0 0,0,0:0:0 17 4038 . T 0 . DP=10;I16=8,2,0,0,335,12149,0,0,554,31538,0,0,215,4881,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,203:9:0 0,3,29:1:0 0,0,0:0:0 17 4039 . G 0 . DP=10;I16=8,1,0,0,302,10798,0,0,494,27938,0,0,186,4130,0,0;QS=2,0;MQSB=1;MQ0F=0 PL:DP:DV 0,24,180:8:0 0,3,31:1:0 0,0,0:0:0 17 4040 . A 0 . DP=10;I16=8,2,0,0,380,14602,0,0,554,31538,0,0,207,4637,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,223:9:0 0,3,41:1:0 0,0,0:0:0 17 4041 . T 0 . DP=10;I16=8,2,0,0,367,13633,0,0,554,31538,0,0,202,4478,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,216:9:0 0,3,40:1:0 0,0,0:0:0 17 4042 . A 0 . DP=10;I16=8,2,0,0,337,11657,0,0,554,31538,0,0,197,4329,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,205:9:0 0,3,34:1:0 0,0,0:0:0 17 4043 . T 0 . DP=10;I16=8,2,0,0,314,10428,0,0,554,31538,0,0,192,4190,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,190:9:0 0,3,35:1:0 0,0,0:0:0 17 4044 . T 0 . DP=10;I16=8,2,0,0,333,11579,0,0,554,31538,0,0,187,4061,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,199:9:0 0,3,37:1:0 0,0,0:0:0 17 4045 . C 0 . DP=10;I16=7,2,0,0,291,10099,0,0,494,27938,0,0,176,3906,0,0;QS=1,0;MQSB=0.714286;MQ0F=0 PL:DP:DV 0,27,204:9:0 0,0,0:0:0 0,0,0:0:0 17 4046 . C 0 . DP=10;I16=8,2,0,0,356,13032,0,0,554,31538,0,0,177,3833,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,212:9:0 0,3,35:1:0 0,0,0:0:0 17 4047 . A 0 . DP=10;I16=8,2,0,0,347,12557,0,0,554,31538,0,0,172,3734,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,204:9:0 0,3,40:1:0 0,0,0:0:0 17 4048 . C 0 . DP=10;I16=8,2,0,0,342,12124,0,0,554,31538,0,0,167,3645,0,0;QS=2,0;MQSB=0.75;MQ0F=0 PL:DP:DV 0,27,202:9:0 0,3,37:1:0 0,0,0:0:0 17 4049 . G 0 . DP=10;I16=7,2,0,0,260,7786,0,0,494,27938,0,0,146,3310,0,0;QS=2,0;MQSB=0.714286;MQ0F=0 PL:DP:DV 0,24,173:8:0 0,3,24:1:0 0,0,0:0:0 17 4050 . C 0 . DP=9;I16=6,2,0,0,291,10813,0,0,434,24338,0,0,157,3495,0,0;QS=1,0;MQSB=0.666667;MQ0F=0 PL:DP:DV 0,24,204:8:0 0,0,0:0:0 0,0,0:0:0 17 4051 . A 0 . DP=8;I16=5,2,0,0,259,9679,0,0,374,20738,0,0,146,3370,0,0;QS=1,0;MQSB=0.6;MQ0F=0 PL:DP:DV 0,21,192:7:0 0,0,0:0:0 0,0,0:0:0 17 4052 . C 0 . DP=8;I16=5,2,0,0,247,9025,0,0,374,20738,0,0,143,3281,0,0;QS=1,0;MQSB=0.6;MQ0F=0 PL:DP:DV 0,21,190:7:0 0,0,0:0:0 0,0,0:0:0 17 4053 . C 0 . DP=8;I16=6,2,0,0,254,9000,0,0,434,24338,0,0,146,3234,0,0;QS=1,0;MQSB=0.666667;MQ0F=0 PL:DP:DV 0,24,184:8:0 0,0,0:0:0 0,0,0:0:0 17 4054 . C 0 . DP=8;I16=3,2,0,0,160,5344,0,0,254,13538,0,0,122,2984,0,0;QS=1,0;MQSB=0.333333;MQ0F=0 PL:DP:DV 0,15,134:5:0 0,0,0:0:0 0,0,0:0:0 17 4055 . G 0 . DP=8;I16=6,2,0,0,230,6982,0,0,434,24338,0,0,138,3066,0,0;QS=1,0;MQSB=0.666667;MQ0F=0 PL:DP:DV 0,24,169:8:0 0,0,0:0:0 0,0,0:0:0 17 4056 . C 0 . DP=8;I16=6,2,0,0,275,10153,0,0,434,24338,0,0,134,2994,0,0;QS=1,0;MQSB=0.666667;MQ0F=0 PL:DP:DV 0,24,197:8:0 0,0,0:0:0 0,0,0:0:0 17 4057 . T 0 . DP=8;I16=5,2,0,0,243,8603,0,0,374,20738,0,0,127,2877,0,0;QS=1,0;MQSB=0.6;MQ0F=0 PL:DP:DV 0,21,182:7:0 0,0,0:0:0 0,0,0:0:0 17 4058 . A 0 . DP=7;I16=4,2,0,0,214,7742,0,0,314,17138,0,0,116,2728,0,0;QS=1,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,18,171:6:0 0,0,0:0:0 0,0,0:0:0 17 4059 . C 0 . DP=6;I16=4,2,0,0,204,7164,0,0,314,17138,0,0,119,2635,0,0;QS=1,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,18,168:6:0 0,0,0:0:0 0,0,0:0:0 17 4060 . A 0 . DP=6;I16=4,2,0,0,227,8683,0,0,314,17138,0,0,115,2501,0,0;QS=1,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,18,177:6:0 0,0,0:0:0 0,0,0:0:0 17 4061 . C 0 . DP=6;I16=4,2,0,0,193,6681,0,0,314,17138,0,0,111,2375,0,0;QS=1,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,18,157:6:0 0,0,0:0:0 0,0,0:0:0 17 4062 . T 0 . DP=6;I16=4,1,0,0,195,7621,0,0,254,13538,0,0,82,1632,0,0;QS=1,0;MQSB=1;MQ0F=0 PL:DP:DV 0,15,151:5:0 0,0,0:0:0 0,0,0:0:0 17 4063 . C 0 . DP=6;I16=4,2,0,0,216,7984,0,0,314,17138,0,0,102,2098,0,0;QS=1,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,18,170:6:0 0,0,0:0:0 0,0,0:0:0 17 4064 . C 0 . DP=6;I16=4,2,0,0,227,8747,0,0,314,17138,0,0,97,1949,0,0;QS=1,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,18,177:6:0 0,0,0:0:0 0,0,0:0:0 17 4065 . T 0 . DP=6;I16=4,2,0,0,202,6880,0,0,314,17138,0,0,92,1810,0,0;QS=1,0;MQSB=0.5;MQ0F=0 PL:DP:DV 0,18,161:6:0 0,0,0:0:0 0,0,0:0:0 17 4066 . T 0 . DP=5;I16=3,2,0,0,180,6554,0,0,254,13538,0,0,88,1680,0,0;QS=1,0;MQSB=0.333333;MQ0F=0 PL:DP:DV 0,15,153:5:0 0,0,0:0:0 0,0,0:0:0 17 4067 . C 0 . DP=5;I16=3,2,0,0,181,6637,0,0,254,13538,0,0,84,1558,0,0;QS=1,0;MQSB=0.333333;MQ0F=0 PL:DP:DV 0,15,153:5:0 0,0,0:0:0 0,0,0:0:0 17 4068 . T 0 . DP=5;I16=3,2,0,0,198,7868,0,0,254,13538,0,0,80,1444,0,0;QS=1,0;MQSB=0.333333;MQ0F=0 PL:DP:DV 0,15,164:5:0 0,0,0:0:0 0,0,0:0:0 17 4069 . T 0 . DP=5;I16=3,2,0,0,177,6325,0,0,254,13538,0,0,76,1338,0,0;QS=1,0;MQSB=0.333333;MQ0F=0 PL:DP:DV 0,15,154:5:0 0,0,0:0:0 0,0,0:0:0 17 4070 . A 0 . DP=5;I16=3,2,0,0,161,5263,0,0,254,13538,0,0,72,1240,0,0;QS=1,0;MQSB=0.333333;MQ0F=0 PL:DP:DV 0,15,140:5:0 0,0,0:0:0 0,0,0:0:0 17 4071 . G 0 . DP=5;I16=3,2,0,0,166,5658,0,0,254,13538,0,0,68,1150,0,0;QS=1,0;MQSB=0.333333;MQ0F=0 PL:DP:DV 0,15,142:5:0 0,0,0:0:0 0,0,0:0:0 17 4072 . G 0 . DP=5;I16=2,2,0,0,138,4974,0,0,194,9938,0,0,55,987,0,0;QS=1,0;MQSB=0;MQ0F=0 PL:DP:DV 0,12,122:4:0 0,0,0:0:0 0,0,0:0:0 17 4073 . G 0 . DP=5;I16=3,2,0,0,156,5082,0,0,254,13538,0,0,60,994,0,0;QS=1,0;MQSB=0.333333;MQ0F=0 PL:DP:DV 0,15,136:5:0 0,0,0:0:0 0,0,0:0:0 17 4074 . C 0 . DP=5;I16=3,2,0,0,160,5602,0,0,254,13538,0,0,56,928,0,0;QS=1,0;MQSB=0.333333;MQ0F=0 PL:DP:DV 0,15,142:5:0 0,0,0:0:0 0,0,0:0:0 17 4075 . T 0 . DP=5;I16=3,2,0,0,187,7069,0,0,254,13538,0,0,52,870,0,0;QS=1,0;MQSB=0.333333;MQ0F=0 PL:DP:DV 0,15,155:5:0 0,0,0:0:0 0,0,0:0:0 17 4076 . G 0 . DP=5;I16=3,2,0,0,174,6298,0,0,254,13538,0,0,48,820,0,0;QS=1,0;MQSB=0.333333;MQ0F=0 PL:DP:DV 0,15,149:5:0 0,0,0:0:0 0,0,0:0:0 17 4077 . A 0 . DP=4;I16=3,1,0,0,138,4810,0,0,194,9938,0,0,44,728,0,0;QS=1,0;MQSB=1;MQ0F=0 PL:DP:DV 0,12,121:4:0 0,0,0:0:0 0,0,0:0:0 17 4078 . T 0 . DP=4;I16=3,1,0,0,143,5173,0,0,194,9938,0,0,40,644,0,0;QS=1,0;MQSB=1;MQ0F=0 PL:DP:DV 0,12,124:4:0 0,0,0:0:0 0,0,0:0:0 17 4079 . A 0 . DP=4;I16=3,1,0,0,121,3847,0,0,194,9938,0,0,36,568,0,0;QS=1,0;MQSB=1;MQ0F=0 PL:DP:DV 0,12,107:4:0 0,0,0:0:0 0,0,0:0:0 17 4080 . T 0 . DP=4;I16=3,0,0,0,106,3778,0,0,134,6338,0,0,25,451,0,0;QS=1,0;MQ0F=0 PL:DP:DV 0,9,87:3:0 0,0,0:0:0 0,0,0:0:0 17 4081 . T 0 . DP=4;I16=3,1,0,0,106,2934,0,0,194,9938,0,0,28,440,0,0;QS=1,0;MQSB=1;MQ0F=0 PL:DP:DV 0,12,94:4:0 0,0,0:0:0 0,0,0:0:0 17 4082 . C 0 . DP=3;I16=2,1,0,0,110,4042,0,0,134,6338,0,0,25,387,0,0;QS=1,0;MQSB=1;MQ0F=0 PL:DP:DV 0,9,103:3:0 0,0,0:0:0 0,0,0:0:0 17 4083 . C 0 . DP=3;I16=2,1,0,0,104,3648,0,0,134,6338,0,0,22,340,0,0;QS=1,0;MQSB=1;MQ0F=0 PL:DP:DV 0,9,98:3:0 0,0,0:0:0 0,0,0:0:0 17 4084 . A 0 . DP=2;I16=1,1,0,0,78,3050,0,0,97,4969,0,0,20,298,0,0;QS=1,0;MQSB=1;MQ0F=0 PL:DP:DV 0,6,74:2:0 0,0,0:0:0 0,0,0:0:0 17 4085 . C 0 . DP=2;I16=1,1,0,0,62,1940,0,0,97,4969,0,0,18,260,0,0;QS=1,0;MQSB=1;MQ0F=0 PL:DP:DV 0,6,62:2:0 0,0,0:0:0 0,0,0:0:0 17 4086 . G 0 . DP=2;I16=1,1,0,0,56,1640,0,0,97,4969,0,0,16,226,0,0;QS=1,0;MQSB=1;MQ0F=0 PL:DP:DV 0,6,56:2:0 0,0,0:0:0 0,0,0:0:0 17 4087 . C 0 . DP=2;I16=1,1,0,0,69,2405,0,0,97,4969,0,0,14,196,0,0;QS=1,0;MQSB=1;MQ0F=0 PL:DP:DV 0,6,68:2:0 0,0,0:0:0 0,0,0:0:0 17 4088 . A 0 . DP=1;I16=1,0,0,0,39,1521,0,0,37,1369,0,0,13,169,0,0;QS=1,0;MQ0F=0 PL:DP:DV 0,3,37:1:0 0,0,0:0:0 0,0,0:0:0 17 4089 . C 0 . DP=1;I16=1,0,0,0,36,1296,0,0,37,1369,0,0,12,144,0,0;QS=1,0;MQ0F=0 PL:DP:DV 0,3,36:1:0 0,0,0:0:0 0,0,0:0:0 17 4090 . C 0 . DP=1;I16=1,0,0,0,33,1089,0,0,37,1369,0,0,11,121,0,0;QS=1,0;MQ0F=0 PL:DP:DV 0,3,33:1:0 0,0,0:0:0 0,0,0:0:0 17 4091 . T 0 . DP=1;I16=1,0,0,0,36,1296,0,0,37,1369,0,0,10,100,0,0;QS=1,0;MQ0F=0 PL:DP:DV 0,3,36:1:0 0,0,0:0:0 0,0,0:0:0 17 4092 . G 0 . DP=1;I16=1,0,0,0,37,1369,0,0,37,1369,0,0,9,81,0,0;QS=1,0;MQ0F=0 PL:DP:DV 0,3,37:1:0 0,0,0:0:0 0,0,0:0:0 17 4093 . C 0 . DP=1;I16=1,0,0,0,35,1225,0,0,37,1369,0,0,8,64,0,0;QS=1,0;MQ0F=0 PL:DP:DV 0,3,35:1:0 0,0,0:0:0 0,0,0:0:0 17 4094 . T 0 . DP=1;I16=1,0,0,0,40,1600,0,0,37,1369,0,0,7,49,0,0;QS=1,0;MQ0F=0 PL:DP:DV 0,3,37:1:0 0,0,0:0:0 0,0,0:0:0 17 4095 . A 0 . DP=1;I16=1,0,0,0,35,1225,0,0,37,1369,0,0,6,36,0,0;QS=1,0;MQ0F=0 PL:DP:DV 0,3,35:1:0 0,0,0:0:0 0,0,0:0:0 17 4096 . C 0 . DP=1;I16=1,0,0,0,32,1024,0,0,37,1369,0,0,5,25,0,0;QS=1,0;MQ0F=0 PL:DP:DV 0,3,32:1:0 0,0,0:0:0 0,0,0:0:0 17 4097 . A 0 . DP=1;I16=1,0,0,0,35,1225,0,0,37,1369,0,0,4,16,0,0;QS=1,0;MQ0F=0 PL:DP:DV 0,3,35:1:0 0,0,0:0:0 0,0,0:0:0 17 4098 . C 0 . DP=1;I16=1,0,0,0,31,961,0,0,37,1369,0,0,3,9,0,0;QS=1,0;MQ0F=0 PL:DP:DV 0,3,31:1:0 0,0,0:0:0 0,0,0:0:0 17 4099 . T 0 . DP=1;I16=1,0,0,0,32,1024,0,0,37,1369,0,0,2,4,0,0;QS=1,0;MQ0F=0 PL:DP:DV 0,3,32:1:0 0,0,0:0:0 0,0,0:0:0 17 4100 . C 0 . DP=1;I16=1,0,0,0,27,729,0,0,37,1369,0,0,1,1,0,0;QS=1,0;MQ0F=0 PL:DP:DV 0,3,27:1:0 0,0,0:0:0 0,0,0:0:0 17 4101 . C 0 . DP=1;I16=1,0,0,0,26,676,0,0,37,1369,0,0,0,0,0,0;QS=1,0;MQ0F=0 PL:DP:DV 0,3,26:1:0 0,0,0:0:0 0,0,0:0:0 bcftools-1.2/test/norm.fa000066400000000000000000000021161246371514100154350ustar00rootroot00000000000000>20 20:1339000-1339300 AGGATGGGGCTCATTAATAGAGCTCCACTTGTCTCCAGAATCACTGGTGAGGAAGGGGAG TGTTGCCCCCACATTCGTGCACAGCAGGGATGGTTCACCGAACTCCACACCAGTCTCTGC AGAGCCTGTTGGGGAGAGGAGGGCTGTGGTTTCTTTGATGGTGTTCACCTGGAGTAGAGC AAGTATTGTCAAAAGGGTCATCCTCGGAGGTTGCAGTGAGCCGAGATCGCACCATTGCAC TGCAGCCTGGGAGACAGAGCAAGACTCCATCTCAAAAAAAAAAAAAAAAAAAAAGGCCAT C >1 1:10143-10443 CTAACCCCTAACCCTAACCCTAACCCTAACCCTAACCTAACCCTAACCCTAACCCTAACC CTAACCCTAACCCTAACCCTAACCCTAACCCCTAACCCTAACCCTAAACCCTAAACCCTA ACCCTAACCCTAACCCTAACCCTAACCCCAACCCCAACCCCAACCCCAACCCCAACCCCA ACCCTAACCCCTAACCCTAACCCTAACCCTACCCTAACCCTAACCCTAACCCTAACCCTA ACCCTAACCCCTAACCCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCC >2 1:1382388-1382602 GGGCGTCTCATAGCTGGAGCAATGGCGAGCGCCTGGACAAGGGAGGGGAAGGGGTTCTTA TTACTGACGCGGGTAGCCCCTACTGCTGTGTGGTTCCCCTATTTTTTTTTTTTTCTTTTT GAGACGGAGTCTCGCTCTGTCACCCAGGCTGGAGTGCAGTGGCACAATCTCGGCTCACTG CAAGCTCCACCTCCTGGGTTCACGCCATTCTCCTG >3 madeup ACTGGACACGTGGACACACACACACACACACACACACACACAGTCAAACCACCTACCAGA >4 20:8917026-8917085 TCCCCTCTTGACCTCTCTCTATTTTTTTTTTTTTTTCTGAGATGGATTTTTGCTCTTGTT >5 20:18724313-18724343 GTCTCAAAAAAAAAAAAAAAAAAAAGAAAAG bcftools-1.2/test/norm.fa.fai000066400000000000000000000001361246371514100161730ustar00rootroot0000000000000020 301 23 60 61 1 300 347 60 61 2 215 673 60 61 3 60 902 60 61 4 60 985 60 61 5 31 1070 31 32 bcftools-1.2/test/norm.merge.out000066400000000000000000000112011246371514100167470ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##contig= ##contig= ##contig= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##INFO= ##FILTER= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT XY00001 XY00002 1 105 . TAAACCCTAAA TAA,TAACCCTAAA 999 PASS INDEL;AN=4;AC=2,2;DP=19;ISTR=SomeString;XRF=1e+06,2e+06,500000;XRI=1111,2222,5555;XRS=AAA,BBB,DDD;XAF=1e+06,500000;XAI=1111,5555;XAS=AAA,DDD;XGF=1e+06,2e+06,3e+06,500000,.,9e+09;XGI=1111,2222,3333,5555,.,9999;XGS=A,B,C,E,.,F GT:PL:DP:FRF:FRI:FRS:FAF:FAI:FAS:FGF:FGI:FGS 1/2:1,2,3,4,.,6:1:1e+06,2e+06,500000:1111,2222,5555:AAAA,BBB,CC:1e+06,500000:1111,5555:A,BB:1e+06,2e+06,3e+06,500000,.,9e+09:1111,2222,3333,5555,.,9999:A,BB,CCC,EEEE,.,FFFFF 1/2:1,2,3,4,.,6:1:1e+06,2e+06,500000:1111,2222,5555:AAAA,BBB,CC:1e+06,500000:1111,5555:A,BB:1e+06,2e+06,3e+06,500000,.,9e+09:1111,2222,3333,5555,.,9999:A,BB,CCC,EEEE,.,FFFFF 2 1 . GGGCGTCTCATAGCTGGAGCAATGGCGAGCGCCTGGACAAGGGAGGGGAAGGGGTTCTTATTACTGACGCGGGTAGCCCCTACTGCTGTGTGGTTCCCCTATTTTTTTTTTTTTCTTTTTGAGACGGAGTCTCGCTCTGTCACCCAGGCTGGAGTGCAGTGGCACAATCTCGGCTCACTGCAAGCTCCACCT ACGT 999 PASS INDEL;AN=4;AC=2 GT:DP 1/0:1 1/0:1 2 101 . ATTTTTTTTTTTTT ATTTTTTTTTTTTTTT 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 2 114 . TC TTCC,TTC 999 FAIL1 INDEL;AN=4;AC=2,2 GT:DP 1/2:1 1/2:1 2 115 . C T 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 20 3 . GATG CTATG,GACT 999 PASS INDEL;AN=4;AC=2,2 GT 2/1 2/1 20 5 id0001;id0002 TGGG TAC,TG,TGGGG,AC . PASS INDEL;AN=4;AC=2,2,0,0 GT:PL:DP 1/2:1,2,3,4,.,6,7,.,.,10,11,.,.,.,15:1 1/2:1,2,3,4,.,6,7,.,.,10,11,.,.,.,15:1 20 59 id0003 AG . 999 PASS AN=4 GT:PL:DP 0/0:0:4 0/0:0:4 20 80 . CACAG CACAT 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 81 . A C 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG ACACCG 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG AAAAAA 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 273 . CAAAAAAAAAAAAAAAAAAAAA CAAAAAAAAAAAAAAAAAAAAAAA,CAAAAAAAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=4;AC=2,2 GT:PL:DP 1/2:0,3,5,3,.,5:1 1/2:0,3,5,3,.,5:1 20 274 . AAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 275 . A C,G 0 FAIL1;FAIL2 INDEL;AN=2;AC=0,2 GT:PL:DP:FGF:FGI:FGS:FSTR 2:0,0,0:0:1e+06,2e+06,3e+06:1111,2222,3333:A,BB,CCC:WORD 2:0,0,0:0:1e+06,2e+06,3e+06:1111,2222,3333:A,BB,CCC:WORD 20 278 . AAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 300 . A C,G 999 PASS INDEL;AN=0;AC=0,0 GT:PL:DP ./.:0,0,0,0,.,0:0 ./.:0,0,0,0,.,0:0 bcftools-1.2/test/norm.merge.strict.out000066400000000000000000000112001246371514100202550ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##contig= ##contig= ##contig= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##INFO= ##FILTER= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT XY00001 XY00002 1 105 . TAAACCCTAAA TAA,TAACCCTAAA 999 PASS INDEL;AN=4;AC=2,2;DP=19;ISTR=SomeString;XRF=1e+06,2e+06,500000;XRI=1111,2222,5555;XRS=AAA,BBB,DDD;XAF=1e+06,500000;XAI=1111,5555;XAS=AAA,DDD;XGF=1e+06,2e+06,3e+06,500000,.,9e+09;XGI=1111,2222,3333,5555,.,9999;XGS=A,B,C,E,.,F GT:PL:DP:FRF:FRI:FRS:FAF:FAI:FAS:FGF:FGI:FGS 1/2:1,2,3,4,.,6:1:1e+06,2e+06,500000:1111,2222,5555:AAAA,BBB,CC:1e+06,500000:1111,5555:A,BB:1e+06,2e+06,3e+06,500000,.,9e+09:1111,2222,3333,5555,.,9999:A,BB,CCC,EEEE,.,FFFFF 1/2:1,2,3,4,.,6:1:1e+06,2e+06,500000:1111,2222,5555:AAAA,BBB,CC:1e+06,500000:1111,5555:A,BB:1e+06,2e+06,3e+06,500000,.,9e+09:1111,2222,3333,5555,.,9999:A,BB,CCC,EEEE,.,FFFFF 2 1 . GGGCGTCTCATAGCTGGAGCAATGGCGAGCGCCTGGACAAGGGAGGGGAAGGGGTTCTTATTACTGACGCGGGTAGCCCCTACTGCTGTGTGGTTCCCCTATTTTTTTTTTTTTCTTTTTGAGACGGAGTCTCGCTCTGTCACCCAGGCTGGAGTGCAGTGGCACAATCTCGGCTCACTGCAAGCTCCACCT ACGT 999 PASS INDEL;AN=4;AC=2 GT:DP 1/0:1 1/0:1 2 101 . ATTTTTTTTTTTTT ATTTTTTTTTTTTTTT 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 2 114 . TC TTCC,TTC 999 PASS INDEL;AN=4;AC=2,2 GT:DP 1/2:1 1/2:1 2 115 . C T 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 20 3 . GATG CTATG,GACT 999 PASS INDEL;AN=4;AC=2,2 GT 2/1 2/1 20 5 id0001;id0002 TGGG TAC,TG,TGGGG,AC . PASS INDEL;AN=4;AC=2,2,0,0 GT:PL:DP 1/2:1,2,3,4,.,6,7,.,.,10,11,.,.,.,15:1 1/2:1,2,3,4,.,6,7,.,.,10,11,.,.,.,15:1 20 59 id0003 AG . 999 PASS AN=4 GT:PL:DP 0/0:0:4 0/0:0:4 20 80 . CACAG CACAT 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 81 . A C 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG ACACCG 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG AAAAAA 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 273 . CAAAAAAAAAAAAAAAAAAAAA CAAAAAAAAAAAAAAAAAAAAAAA,CAAAAAAAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=4;AC=2,2 GT:PL:DP 1/2:0,3,5,3,.,5:1 1/2:0,3,5,3,.,5:1 20 274 . AAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 275 . A C,G 0 FAIL1;FAIL2 INDEL;AN=2;AC=0,2 GT:PL:DP:FGF:FGI:FGS:FSTR 2:0,0,0:0:1e+06,2e+06,3e+06:1111,2222,3333:A,BB,CCC:WORD 2:0,0,0:0:1e+06,2e+06,3e+06:1111,2222,3333:A,BB,CCC:WORD 20 278 . AAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 300 . A C,G 999 PASS INDEL;AN=0;AC=0,0 GT:PL:DP ./.:0,0,0,0,.,0:0 ./.:0,0,0,0,.,0:0 bcftools-1.2/test/norm.merge.vcf000066400000000000000000000124731246371514100167320ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##contig= ##contig= ##contig= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##INFO= ##FILTER= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT XY00001 XY00002 1 105 . TAAACCCTAAA TAA 999 PASS INDEL;AN=4;AC=2;DP=19;ISTR=SomeString;XRF=1e+06,2e+06;XRI=1111,2222;XRS=AAA,BBB;XAF=1e+06;XAI=1111;XAS=AAA;XGF=1e+06,2e+06,3e+06;XGI=1111,2222,3333;XGS=A,B,C GT:PL:DP:FRF:FRI:FRS:FAF:FAI:FAS:FGF:FGI:FGS 1/0:1,2,3:1:1e+06,2e+06:1111,2222:AAAA,BBB:1e+06:1111:A:1e+06,2e+06,3e+06:1111,2222,3333:A,BB,CCC 1/0:1,2,3:1:1e+06,2e+06:1111,2222:AAAA,BBB:1e+06:1111:A:1e+06,2e+06,3e+06:1111,2222,3333:A,BB,CCC 1 105 . TAAACCCTAAA TAACCCTAAA 999 PASS INDEL;AN=4;AC=2;DP=19;ISTR=SomeString;XRF=1e+06,500000;XRI=1111,5555;XRS=AAA,DDD;XAF=500000;XAI=5555;XAS=DDD;XGF=1e+06,500000,9e+09;XGI=1111,5555,9999;XGS=A,E,F GT:PL:DP:FRF:FRI:FRS:FAF:FAI:FAS:FGF:FGI:FGS 0/1:1,4,6:1:1e+06,500000:1111,5555:AAAA,CC:500000:5555:BB:1e+06,500000,9e+09:1111,5555,9999:A,EEEE,FFFFF 0/1:1,4,6:1:1e+06,500000:1111,5555:AAAA,CC:500000:5555:BB:1e+06,500000,9e+09:1111,5555,9999:A,EEEE,FFFFF 2 1 . GGGCGTCTCATAGCTGGAGCAATGGCGAGCGCCTGGACAAGGGAGGGGAAGGGGTTCTTATTACTGACGCGGGTAGCCCCTACTGCTGTGTGGTTCCCCTATTTTTTTTTTTTTCTTTTTGAGACGGAGTCTCGCTCTGTCACCCAGGCTGGAGTGCAGTGGCACAATCTCGGCTCACTGCAAGCTCCACCT ACGT 999 PASS INDEL;AN=4;AC=2 GT:DP 1/0:1 1/0:1 2 101 . ATTTTTTTTTTTTT ATTTTTTTTTTTTTTT 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 2 114 . TC TTCC 999 FAIL1 INDEL;AN=4;AC=2 GT:DP 1/0:1 1/0:1 2 114 . TC TTC 999 PASS INDEL;AN=4;AC=2 GT:DP 0/1:1 0/1:1 2 115 . C T 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 20 3 . G CT 999 PASS INDEL;AN=4;AC=2 GT 0/1 0/1 20 3 . GATG GACT 999 PASS INDEL;AN=4;AC=2 GT 1/0 1/0 20 5 . TGGG TAC . PASS INDEL;AN=4;AC=2 GT:PL:DP 1/0:1,2,3:1 1/0:1,2,3:1 20 5 id0001 TGGG TG . PASS INDEL;AN=4;AC=2 GT:PL:DP 0/1:1,4,6:1 0/1:1,4,6:1 20 5 id0002 TGGG TGGGG . PASS INDEL;AN=4;AC=0 GT:PL:DP 0/0:1,7,10:1 0/0:1,7,10:1 20 5 . TGGG AC . PASS INDEL;AN=4;AC=0 GT:PL:DP 0/0:1,11,15:1 0/0:1,11,15:1 20 59 id0003 AG . 999 PASS AN=4 GT:PL:DP 0/0:0:4 0/0:0:4 20 80 . CACAG CACAT 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 81 . A C 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG ACACCG 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG AAAAAA 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 273 . CAAAAAAAAAAAAAAAAAAAAA CAAAAAAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=4;AC=2 GT:PL:DP 1/0:0,3,5:1 1/0:0,3,5:1 20 273 . CAAAAAAAAAAAAAAAAAAAAA CAAAAAAAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=4;AC=2 GT:PL:DP 0/1:0,3,5:1 0/1:0,3,5:1 20 274 . AAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 275 . A C 0 FAIL1 INDEL;AN=2;AC=0 GT:PL:DP:FGF:FGI:FGS:FSTR 0:0,0:0:1e+06,2e+06:1111,2222:A,BB:WORD 0:0,0:0:1e+06,2e+06:1111,2222:A,BB:WORD 20 275 . A G 0 FAIL2 INDEL;AN=2;AC=2 GT:PL:DP:FGF:FGI:FGS:FSTR 1:0,0:0:1e+06,3e+06:1111,3333:A,CCC:WORD 1:0,0:0:1e+06,3e+06:1111,3333:A,CCC:WORD 20 278 . AAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 300 . A C 998 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 300 . A G 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 bcftools-1.2/test/norm.out000066400000000000000000000112551246371514100156620ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##contig= ##contig= ##contig= ##contig= ##contig= ##contig= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT XY00001 XY00002 1 105 . TAAACCCTA T,TAACCCTA 999 PASS INDEL;AN=4;AC=2,2;DP=19;ISTR=SomeString;XRF=1e+06,2e+06,500000;XRI=1111,2222,5555;XRS=AAA,BBB,DDD;XAF=1e+06,500000;XAI=1111,5555;XAS=AAA,DDD;XGF=1e+06,2e+06,3e+06,500000,.,9e+09;XGI=1111,2222,3333,5555,.,9999;XGS=A,B,C,E,.,F GT:PL:DP:FRF:FRI:FRS:FAF:FAI:FAS:FGF:FGI:FGS 1/2:1,2,3,4,5,6:1:1e+06,2e+06,500000:1111,2222,5555:AAAA,BBB,CC:1e+06,500000:1111,5555:A,BB:1e+06,2e+06,3e+06,500000,.,9e+09:1111,2222,3333,5555,.,9999:A,BB,CCC,EEEE,.,FFFFF 1/2:1,2,3,4,5,6:1:1e+06,2e+06,500000:1111,2222,5555:AAAA,BBB,CC:1e+06,500000:1111,5555:A,BB:1e+06,2e+06,3e+06,500000,.,9e+09:1111,2222,3333,5555,.,9999:A,BB,CCC,EEEE,.,FFFFF 2 1 . GGGCGTCTCATAGCTGGAGCAATGGCGAGCGCCTGGACAAGGGAGGGGAAGGGGTTCTTATTACTGACGCGGGTAGCCCCTACTGCTGTGTGGTTCCCCTATTTTTTTTTTTTTCTTTTTGAGACGGAGTCTCGCTCTGTCACCCAGGCTGGAGTGCAGTGGCACAATCTCGGCTCACTGCAAGCTCCACC ACG 999 PASS INDEL;AN=4;AC=2 GT:DP 1/0:1 1/0:1 2 101 . A ATT 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 2 114 . T TTC,TT 999 PASS INDEL;AN=4;AC=2,2 GT:DP 1/2:1 1/2:1 2 115 . C T 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 20 3 . G CT 999 PASS INDEL;AN=4;AC=2 GT 0/1 0/1 20 5 . TG CT 999 PASS INDEL;AN=4;AC=2 GT 1/0 1/0 20 5 . TGGG TAC,TG,TGGGG,AC . PASS INDEL;AN=4;AC=2,2,0,0 GT:PL:DP 1/2:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15:1 1/2:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15:1 20 59 . AG . 999 PASS AN=4 GT:PL:DP 0/0:0:4 0/0:0:4 20 81 . A C 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 84 . G T 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . T A 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG AAAAAA 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 273 . C CAA,CAAA 999 PASS INDEL;AN=4;AC=2,2 GT:PL:DP 1/2:0,3,5,3,5,5:1 1/2:0,3,5,3,5,5:1 20 273 . C CAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 273 . C CAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 275 . A C,G 999 PASS INDEL;AN=2;AC=0,2 GT:PL:DP:FGF:FGI:FGS:FSTR 2:0,0,0:0:1e+06,2e+06,3e+06:1111,2222,3333:A,BB,CCC:WORD 2:0,0,0:0:1e+06,2e+06,3e+06:1111,2222,3333:A,BB,CCC:WORD 3 10 . GTGGAC GTGGACACAC,GTGGACAC,GTGGACACACAC,GTGG,GTGGACACACACAC,ATGGACACACAC 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 3 17 . CA C 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 4 36 . TC C,TT,TTC 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 5 21 . A AAG 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 bcftools-1.2/test/norm.split.out000066400000000000000000000123111246371514100170060ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##contig= ##contig= ##contig= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT XY00001 XY00002 1 105 . TAAACCCTAAA TAA 999 PASS INDEL;AN=4;AC=2;DP=19;ISTR=SomeString;XRF=1e+06,2e+06;XRI=1111,2222;XRS=AAA,BBB;XAF=1e+06;XAI=1111;XAS=AAA;XGF=1e+06,2e+06,3e+06;XGI=1111,2222,3333;XGS=A,B,C GT:PL:DP:FRF:FRI:FRS:FAF:FAI:FAS:FGF:FGI:FGS 1/0:1,2,3:1:1e+06,2e+06:1111,2222:AAAA,BBB:1e+06:1111:A:1e+06,2e+06,3e+06:1111,2222,3333:A,BB,CCC 1/0:1,2,3:1:1e+06,2e+06:1111,2222:AAAA,BBB:1e+06:1111:A:1e+06,2e+06,3e+06:1111,2222,3333:A,BB,CCC 1 105 . TAAACCCTAAA TAACCCTAAA 999 PASS INDEL;AN=4;AC=2;DP=19;ISTR=SomeString;XRF=1e+06,500000;XRI=1111,5555;XRS=AAA,DDD;XAF=500000;XAI=5555;XAS=DDD;XGF=1e+06,500000,9e+09;XGI=1111,5555,9999;XGS=A,E,F GT:PL:DP:FRF:FRI:FRS:FAF:FAI:FAS:FGF:FGI:FGS 0/1:1,4,6:1:1e+06,500000:1111,5555:AAAA,CC:500000:5555:BB:1e+06,500000,9e+09:1111,5555,9999:A,EEEE,FFFFF 0/1:1,4,6:1:1e+06,500000:1111,5555:AAAA,CC:500000:5555:BB:1e+06,500000,9e+09:1111,5555,9999:A,EEEE,FFFFF 2 1 . GGGCGTCTCATAGCTGGAGCAATGGCGAGCGCCTGGACAAGGGAGGGGAAGGGGTTCTTATTACTGACGCGGGTAGCCCCTACTGCTGTGTGGTTCCCCTATTTTTTTTTTTTTCTTTTTGAGACGGAGTCTCGCTCTGTCACCCAGGCTGGAGTGCAGTGGCACAATCTCGGCTCACTGCAAGCTCCACCT ACGT 999 PASS INDEL;AN=4;AC=2 GT:DP 1/0:1 1/0:1 2 101 . ATTTTTTTTTTTTT ATTTTTTTTTTTTTTT 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 2 114 . TC TTCC 999 PASS INDEL;AN=4;AC=2 GT:DP 1/0:1 1/0:1 2 114 . TC TTC 999 PASS INDEL;AN=4;AC=2 GT:DP 0/1:1 0/1:1 2 115 . C T 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 20 3 . G CT 999 PASS INDEL;AN=4;AC=2 GT 0/1 0/1 20 3 . GATG GACT 999 PASS INDEL;AN=4;AC=2 GT 1/0 1/0 20 5 . TGGG TAC . PASS INDEL;AN=4;AC=2 GT:PL:DP 1/0:1,2,3:1 1/0:1,2,3:1 20 5 . TGGG TG . PASS INDEL;AN=4;AC=2 GT:PL:DP 0/1:1,4,6:1 0/1:1,4,6:1 20 5 . TGGG TGGGG . PASS INDEL;AN=4;AC=0 GT:PL:DP 0/0:1,7,10:1 0/0:1,7,10:1 20 5 . TGGG AC . PASS INDEL;AN=4;AC=0 GT:PL:DP 0/0:1,11,15:1 0/0:1,11,15:1 20 59 . AG . 999 PASS AN=4 GT:PL:DP 0/0:0:4 0/0:0:4 20 80 . CACAG CACAT 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 81 . A C 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG ACACCG 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG AAAAAA 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 273 . CAAAAAAAAAAAAAAAAAAAAA CAAAAAAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=4;AC=2 GT:PL:DP 1/0:0,3,5:1 1/0:0,3,5:1 20 273 . CAAAAAAAAAAAAAAAAAAAAA CAAAAAAAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=4;AC=2 GT:PL:DP 0/1:0,3,5:1 0/1:0,3,5:1 20 274 . AAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 275 . A C 999 PASS INDEL;AN=2;AC=0 GT:PL:DP:FGF:FGI:FGS:FSTR 0:0,0:0:1e+06,2e+06:1111,2222:A,BB:WORD 0:0,0:0:1e+06,2e+06:1111,2222:A,BB:WORD 20 275 . A G 999 PASS INDEL;AN=2;AC=2 GT:PL:DP:FGF:FGI:FGS:FSTR 1:0,0:0:1e+06,3e+06:1111,3333:A,CCC:WORD 1:0,0:0:1e+06,3e+06:1111,3333:A,CCC:WORD 20 278 . AAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 300 . A C 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 300 . A G 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 bcftools-1.2/test/norm.split.vcf000066400000000000000000000110641246371514100167610ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##contig= ##contig= ##contig= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT XY00001 XY00002 1 105 . TAAACCCTAAA TAA,TAACCCTAAA 999 PASS INDEL;AN=4;AC=2,2;DP=19;ISTR=SomeString;XRF=1e+06,2e+06,500000;XRI=1111,2222,5555;XRS=AAA,BBB,DDD;XAF=1e+06,500000;XAI=1111,5555;XAS=AAA,DDD;XGF=1e+06,2e+06,3e+06,500000,.,9e+09;XGI=1111,2222,3333,5555,.,9999;XGS=A,B,C,E,.,F GT:PL:DP:FRF:FRI:FRS:FAF:FAI:FAS:FGF:FGI:FGS 1/2:1,2,3,4,5,6:1:1e+06,2e+06,500000:1111,2222,5555:AAAA,BBB,CC:1e+06,500000:1111,5555:A,BB:1e+06,2e+06,3e+06,500000,.,9e+09:1111,2222,3333,5555,.,9999:A,BB,CCC,EEEE,.,FFFFF 1/2:1,2,3,4,5,6:1:1e+06,2e+06,500000:1111,2222,5555:AAAA,BBB,CC:1e+06,500000:1111,5555:A,BB:1e+06,2e+06,3e+06,500000,.,9e+09:1111,2222,3333,5555,.,9999:A,BB,CCC,EEEE,.,FFFFF 2 1 . GGGCGTCTCATAGCTGGAGCAATGGCGAGCGCCTGGACAAGGGAGGGGAAGGGGTTCTTATTACTGACGCGGGTAGCCCCTACTGCTGTGTGGTTCCCCTATTTTTTTTTTTTTCTTTTTGAGACGGAGTCTCGCTCTGTCACCCAGGCTGGAGTGCAGTGGCACAATCTCGGCTCACTGCAAGCTCCACCT ACGT 999 PASS INDEL;AN=4;AC=2 GT:DP 1/0:1 1/0:1 2 101 . ATTTTTTTTTTTTT ATTTTTTTTTTTTTTT 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 2 114 . TC TTCC,TTC 999 PASS INDEL;AN=4;AC=2,2 GT:DP 1/2:1 1/2:1 2 115 . C T 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 20 3 . G CT 999 PASS INDEL;AN=4;AC=2 GT 0/1 0/1 20 3 . GATG GACT 999 PASS INDEL;AN=4;AC=2 GT 1/0 1/0 20 5 . TGGG TAC,TG,TGGGG,AC . PASS INDEL;AN=4;AC=2,2,0,0 GT:PL:DP 1/2:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15:1 1/2:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15:1 20 59 . AG . 999 PASS AN=4 GT:PL:DP 0/0:0:4 0/0:0:4 20 80 . CACAG CACAT 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 81 . A C 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG ACACCG 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG AAAAAA 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 273 . CAAAAAAAAAAAAAAAAAAAAA CAAAAAAAAAAAAAAAAAAAAAAA,CAAAAAAAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=4;AC=2,2 GT:PL:DP 1/2:0,3,5,3,5,5:1 1/2:0,3,5,3,5,5:1 20 274 . AAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 275 . A C,G 999 PASS INDEL;AN=2;AC=0,2 GT:PL:DP:FGF:FGI:FGS:FSTR 2:0,0,0:0:1e+06,2e+06,3e+06:1111,2222,3333:A,BB,CCC:WORD 2:0,0,0:0:1e+06,2e+06,3e+06:1111,2222,3333:A,BB,CCC:WORD 20 278 . AAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 300 . A C,G 999 PASS INDEL;AN=0;AC=0,0 GT:PL:DP ./.:0,0,0,0,0,0:0 ./.:0,0,0,0,0,0:0 bcftools-1.2/test/norm.vcf000066400000000000000000000116271246371514100156340ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##contig= ##contig= ##contig= ##contig= ##contig= ##contig= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT XY00001 XY00002 1 105 . TAAACCCTAAA TAA,TAACCCTAAA 999 PASS INDEL;AN=4;AC=2,2;DP=19;ISTR=SomeString;XRF=1e+06,2e+06,500000;XRI=1111,2222,5555;XRS=AAA,BBB,DDD;XAF=1e+06,500000;XAI=1111,5555;XAS=AAA,DDD;XGF=1e+06,2e+06,3e+06,500000,.,9e+09;XGI=1111,2222,3333,5555,.,9999;XGS=A,B,C,E,.,F GT:PL:DP:FRF:FRI:FRS:FAF:FAI:FAS:FGF:FGI:FGS 1/2:1,2,3,4,5,6:1:1e+06,2e+06,500000:1111,2222,5555:AAAA,BBB,CC:1e+06,500000:1111,5555:A,BB:1e+06,2e+06,3e+06,500000,.,9e+09:1111,2222,3333,5555,.,9999:A,BB,CCC,EEEE,.,FFFFF 1/2:1,2,3,4,5,6:1:1e+06,2e+06,500000:1111,2222,5555:AAAA,BBB,CC:1e+06,500000:1111,5555:A,BB:1e+06,2e+06,3e+06,500000,.,9e+09:1111,2222,3333,5555,.,9999:A,BB,CCC,EEEE,.,FFFFF 2 1 . GGGCGTCTCATAGCTGGAGCAATGGCGAGCGCCTGGACAAGGGAGGGGAAGGGGTTCTTATTACTGACGCGGGTAGCCCCTACTGCTGTGTGGTTCCCCTATTTTTTTTTTTTTCTTTTTGAGACGGAGTCTCGCTCTGTCACCCAGGCTGGAGTGCAGTGGCACAATCTCGGCTCACTGCAAGCTCCACCT ACGT 999 PASS INDEL;AN=4;AC=2 GT:DP 1/0:1 1/0:1 2 101 . ATTTTTTTTTTTTT ATTTTTTTTTTTTTTT 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 2 114 . TC TTCC,TTC 999 PASS INDEL;AN=4;AC=2,2 GT:DP 1/2:1 1/2:1 2 115 . C T 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 20 3 . G CT 999 PASS INDEL;AN=4;AC=2 GT 0/1 0/1 20 3 . GATG GACT 999 PASS INDEL;AN=4;AC=2 GT 1/0 1/0 20 5 . TGGG TAC,TG,TGGGG,AC . PASS INDEL;AN=4;AC=2,2,0,0 GT:PL:DP 1/2:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15:1 1/2:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15:1 20 59 . AG . 999 PASS AN=4 GT:PL:DP 0/0:0:4 0/0:0:4 20 80 . CACAG CACAT 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 81 . A C 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG ACACCG 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG AAAAAA 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 273 . CAAAAAAAAAAAAAAAAAAAAA CAAAAAAAAAAAAAAAAAAAAAAA,CAAAAAAAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=4;AC=2,2 GT:PL:DP 1/2:0,3,5,3,5,5:1 1/2:0,3,5,3,5,5:1 20 274 . AAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 275 . A C,G 999 PASS INDEL;AN=2;AC=0,2 GT:PL:DP:FGF:FGI:FGS:FSTR 2:0,0,0:0:1e+06,2e+06,3e+06:1111,2222,3333:A,BB,CCC:WORD 2:0,0,0:0:1e+06,2e+06,3e+06:1111,2222,3333:A,BB,CCC:WORD 20 278 . AAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 3 10 . GTGGAC GTGGACACAC,GTGGACAC,GTGGACACACAC,GTGG,GTGGACACACACAC,ATGGACACACAC 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 3 15 . CACA CAC 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 4 21 . ATTTTTTTTTTTTTTTC ATTTTTTTTTTTTTTC,ATTTTTTTTTTTTTTTT,ATTTTTTTTTTTTTTTTC 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 5 22 . A AGA 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 bcftools-1.2/test/plugin-missing2ref.out.vcf000066400000000000000000000041271246371514100212100ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##test= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= ##bcftools_pluginCommand=plugin missing2ref -o test.out.vcf -- missing2ref -p -m #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B C D 1 3000150 . C T 59.2 PASS . GT:GQ 1|1:245 1|1:245 0/1:245 1/1:245 1 3000151 . C T 59.2 PASS . GT:DP:GQ 0|0:32:245 0|0:32:245 0|0:32:245 0|0:32:245 1 3062915 id3D GTTT G 12.9 q10 DP4=1,2,3,4;INDEL;STR=test GT:GQ:DP:GL 0/1:409:35:-20,-5,-20 0/1:409:35:-20,-5,-20 0/1:409:35:-20,-5,-20 0/1:409:35:-20,-5,-20 1 3062915 idSNP G T,C 12.6 test TEST=5;DP4=1,2,3,4 GT:TT:GQ:DP:GL 0/1:0,1:409:35:-20,-5,-20,-20,-5,-20 2:0,1:409:35:-20,-5,-20 2/2:0,1:409:35:-20,-5,-20 2|2:0,1:409:35:-20,-5,-20 2 3199812 . G GTT,GT 82.7 PASS . GT:GQ:DP 0|0:322:26 0|0:322:26 0|0:322:26 0|0:322:26 3 3212016 . CTT C,CT 79 PASS . GT:GQ:DP 0|0:91:26 0|0:91:26 0|0:91:26 0|0:91:26 4 3258448 . TACACACAC T 59.9 PASS . GT:GQ:DP 0|0:325:31 0|0:325:31 0|0:325:31 0|0:325:31 bcftools-1.2/test/plugin-missing2ref.vcf000066400000000000000000000040061246371514100203760ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##test= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B C D 1 3000150 . C T 59.2 PASS . GT:GQ ./.:245 ./.:245 0/1:245 1/1:245 1 3000151 . C T 59.2 PASS . GT:DP:GQ ./.:32:245 ./.:32:245 0|0:32:245 0|0:32:245 1 3062915 id3D GTTT G 12.9 q10 DP4=1,2,3,4;INDEL;STR=test GT:GQ:DP:GL 0/1:409:35:-20,-5,-20 0/1:409:35:-20,-5,-20 0/1:409:35:-20,-5,-20 0/1:409:35:-20,-5,-20 1 3062915 idSNP G T,C 12.6 test TEST=5;DP4=1,2,3,4 GT:TT:GQ:DP:GL 0/1:0,1:409:35:-20,-5,-20,-20,-5,-20 2:0,1:409:35:-20,-5,-20 2/2:0,1:409:35:-20,-5,-20 ./.:0,1:409:35:-20,-5,-20 2 3199812 . G GTT,GT 82.7 PASS . GT:GQ:DP ./.:322:26 ./.:322:26 ./.:322:26 ./.:322:26 3 3212016 . CTT C,CT 79 PASS . GT:GQ:DP ./.:91:26 ./.:91:26 ./.:91:26 ./.:91:26 4 3258448 . TACACACAC T 59.9 PASS . GT:GQ:DP ./.:325:31 ./.:325:31 ./.:325:31 ./.:325:31 bcftools-1.2/test/plugin1.vcf000066400000000000000000000043351246371514100162360ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##FORMAT= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##test= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3000150 . C T 59.2 PASS . GT:GQ ./.:245 ./.:245 1 3000151 . C T 59.2 PASS . GT:DP:GQ ./.:32:245 ./.:32:245 1 3062915 id3D GTTT G 12.9 q10 DP4=1,2,3,4;INDEL;STR=test GT:GQ:DP:GL 0/1:409:35:-20,-5,-20 0/1:409:35:-20,-5,-20 1 3062915 idSNP G T,C 12.6 test TEST=5;DP4=1,2,3,4 GT:TT:GQ:DP:GL 0/1:0,1:409:35:-20,-5,-20,-20,-5,-20 2:0,1:409:35:-20,-5,-20 1 3106154 . CAAA C 342 PASS . GT:GQ:DP ./.:245:32 ./.:245:32 1 3106154 . C CT 59.2 PASS . GT:GQ:DP ./.:245:32 ./.:245:32 1 3157410 . GA G 90.6 q10 . GT:GQ:DP 1/1:21:21 1/1:21:21 1 3162006 . GAA G 60.2 PASS . GT:GQ:DP ./.:212:22 ./.:212:22 1 3177144 . G T 45 PASS . GT:GQ:DP ./.:150:30 ./.:150:30 1 3177144 . G . 45 PASS . GT:GQ:DP ./.:150:30 ./.:150:30 1 3184885 . TAAAA TA,T 61.5 PASS . GT:GQ:DP ./.:12:10 ./.:12:10 2 3199812 . G GTT,GT 82.7 PASS . GT:GQ:DP ./.:322:26 ./.:322:26 3 3212016 . CTT C,CT 79 PASS . GT:GQ:DP ./.:91:26 ./.:91:26 4 3258448 . TACACACAC T 59.9 PASS . GT:GQ:DP ./.:325:31 ./.:325:31 bcftools-1.2/test/query.10.out000066400000000000000000000000201246371514100162570ustar00rootroot000000000000003258449 1/1 0/1 bcftools-1.2/test/query.11.out000066400000000000000000000000401246371514100162620ustar00rootroot000000000000003062915 0/1 0/2 3258449 1/1 0/1 bcftools-1.2/test/query.12.out000066400000000000000000000010031246371514100162630ustar00rootroot00000000000000I8=. I16=. I32=. IF=. IA8=. IA16=. IA32=. IAF=. IA8=. IA16=. IA32=. IAF=. .:.:.:. .:.:.:. I8=. I16=. I32=. IF=. IA8=. IA16=. IA32=. IAF=. IA8=. IA16=. IA32=. IAF=. .:.:.:. .:.:.:. I8=. I16=. I32=. IF=. IA8=10,. IA16=1000,. IA32=100000,. IAF=0.003,. IA8=. IA16=. IA32=. IAF=. 10:1000:100000:0.003 .:.:.:. I8=. I16=. I32=. IF=. IA8=. IA16=. IA32=. IAF=. IA8=. IA16=. IA32=. IAF=. 10:1000:100000:0.003 .:.:.:. I8=. I16=. I32=. IF=. IA8=. IA16=. IA32=. IAF=. IA8=. IA16=. IA32=. IAF=. 10:1000:100000:0.003 .:.:.:. bcftools-1.2/test/query.13.out000066400000000000000000000000441246371514100162700ustar00rootroot000000000000003000151 1 0 3000152 0 1 3000153 1 1 bcftools-1.2/test/query.14.out000066400000000000000000000000441246371514100162710ustar00rootroot000000000000003000150 0 0 3000151 1 0 3000152 0 1 bcftools-1.2/test/query.15.out000066400000000000000000000000141246371514100162670ustar00rootroot000000000000003000150 0 0 bcftools-1.2/test/query.16.out000066400000000000000000000000141246371514100162700ustar00rootroot000000000000003000153 1 1 bcftools-1.2/test/query.17.out000066400000000000000000000000101246371514100162650ustar00rootroot000000000000001 2 3 4 bcftools-1.2/test/query.2.out000066400000000000000000000000171246371514100162060ustar00rootroot000000000000001111,2222,3333 bcftools-1.2/test/query.2.vcf000066400000000000000000000012271246371514100161610ustar00rootroot00000000000000##fileformat=VCFv4.1 ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT C D 1 3062915 . GTTT G 48.7 . XX_A=1;XX.A=2;XX.A0=3;xx.a0=4 GT 0/1 0/1 bcftools-1.2/test/query.3.out000066400000000000000000000000221246371514100162030ustar00rootroot000000000000001e+06,2e+06,3e+06 bcftools-1.2/test/query.4.out000066400000000000000000000000301246371514100162030ustar00rootroot00000000000000ABC,DEF,GHI,JKL,MNO,PQR bcftools-1.2/test/query.5.out000066400000000000000000000000361246371514100162120ustar00rootroot000000000000003106154 C CT 3212016 CTT C,CT bcftools-1.2/test/query.6.out000066400000000000000000000001031246371514100162060ustar00rootroot000000000000003062915 G C,T 3184885 TAAAA TA,T 3199812 G GTT,GT 3212016 CTT C,CT bcftools-1.2/test/query.7.out000066400000000000000000000000121246371514100162060ustar00rootroot000000000000003106154 0 bcftools-1.2/test/query.8.out000066400000000000000000000001601246371514100162130ustar00rootroot000000000000003062915 -20,-5,-20 -10,-5,-20 3062915 -20,-5,-20,-20,-5,-20 -10,-5,-20,-20,-5,-20 3062915 -20,-5,-20 -10,-5,-20 bcftools-1.2/test/query.9.out000066400000000000000000000000241246371514100162130ustar00rootroot000000000000003162007 1X4M,1X3M1X bcftools-1.2/test/query.filter.vcf000066400000000000000000000022451246371514100173060ustar00rootroot00000000000000##fileformat=VCFv4.1 ##INFO= ##FORMAT= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3000150 . C T 59.2 PASS . GT 0 0 1 3000151 . C T 59.2 PASS . GT 1 0 1 3000152 . C T 59.2 PASS . GT 0 1 1 3000153 . C T 59.2 PASS . GT 1 1 bcftools-1.2/test/query.out000066400000000000000000000010301246371514100160420ustar00rootroot000000000000001 3062915 GTTT G 1,2,3,4 4 0/1 GTTT/G 0/1 GTTT/G 1 3062915 G C,T 1,2,3,4 4 0/1 G/C 0/2 G/T 1 3062915 GTT G 1,2,3,4 4 0/1 GTT/G 0/1 GTT/G 1 3106154 CAAA C . 0 . . ./. ./. 1 3106154 C CT . 4 0/1 C/CT 0/1 C/CT 1 3157410 G T . 4 1/1 T/T 1/1 T/T 1 3162006 GAA G . 4 0/1 GAA/G 0/1 GAA/G 1 3177144 G . . 4 0/0 G/G 0/0 G/G 1 3184885 TAAAA TA,T . 4 1/2 TA/T 1/2 TA/T 2 3199812 G GTT,GT . 4 1/2 GTT/GT 1/2 GTT/GT 3 3212016 CTT C,CT . 4 1/2 C/CT 1/2 C/CT 4 3258448 TACACACAC T . 4 0/1 TACACACAC/T 0/1 TACACACAC/T 4 3258449 A C . 4 1/1 C/C 0/1 A/C bcftools-1.2/test/query.vcf000066400000000000000000000044101246371514100160160ustar00rootroot00000000000000##fileformat=VCFv4.1 ##INFO= ##FORMAT= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##readme=AAAAAA ##readme=BBBBBB ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT C D 1 3062915 id3D GTTT G 48.7 q10 DP4=1,2,3,4;AN=4;AC=2 GT:GQ:DP:GL 0/1:409:35:-20,-5,-20 0/1:109:25:-10,-5,-20 1 3062915 idSNP G C,T 419 test TEST=5;DP4=1,2,3,4;AN=4;AC=1,1 GT:TT:GQ:DP:GL 0/1:0,1:409:35:-20,-5,-20,-20,-5,-20 0/2:0,1:109:35:-10,-5,-20,-20,-5,-20 1 3062915 id2D GTT G 999 q10 DP4=1,2,3,4;AN=4;AC=2 GT:GQ:DP:GL 0/1:409:35:-20,-5,-20 0/1:109:25:-10,-5,-20 1 3106154 . CAAA C 72.6 PASS AN=0;AC=0 GT:GQ:DP .:245:32 ./.:145:22 1 3106154 . C CT 459 PASS AN=4;AC=2 GT:GQ:DP 0/1:245:32 0/1:145:22 1 3157410 . G T 46.7 q10 AN=4;AC=4 GT:GQ:DP 1/1:21:21 1/1:11:11 1 3162006 . GAA G 206 PASS AN=4;AC=2 GT:GQ:DP 0/1:212:22 0/1:112:12 1 3177144 . G . 364 PASS AN=4;AC=0 GT:GQ:DP 0/0:150:30 0/0:150:20 1 3184885 . TAAAA TA,T 8.42 PASS AN=4;AC=2,2 GT:GQ:DP 1/2:22:20 1/2:12:10 2 3199812 . G GTT,GT 291 PASS AN=4;AC=2,2 GT:GQ:DP 1/2:322:26 1/2:122:16 3 3212016 . CTT C,CT 52.5 PASS AN=4;AC=2,2 GT:GQ:DP 1/2:91:26 1/2:11:16 4 3258448 . TACACACAC T 123 PASS AN=4;AC=2 GT:GQ:DP 0/1:325:31 0/1:125:11 4 3258449 . A C 123 PASS AN=4;AC=3 GT:GQ:DP 1/1:325:31 0/1:125:11 bcftools-1.2/test/regions.out000066400000000000000000000002231246371514100163460ustar00rootroot000000000000001 3062915 GTT,G 1 3062915 G,T 1 3106154 CA,C 1 3106154 C,T,CT 1 3157410 G,A 1 3162006 G,A 1 3184885 T,TA 2 3199815 C,T 3 3212016 C,A 3 3212036 C,A bcftools-1.2/test/regions.tab000066400000000000000000000002201246371514100163020ustar00rootroot000000000000001 3062915 3062915 1 3106154 3106154 1 3157410 3157410 1 3162006 3162006 1 3184885 3184885 2 3199815 3199815 3 3212016 3212016 3 3212036 3212036 bcftools-1.2/test/regions.vcf000066400000000000000000000034411246371514100163220ustar00rootroot00000000000000##fileformat=VCFv4.1 ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##test= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= ##contig= ##contig= ##contig= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A 1 3062915 . GTT G 1806 q10 DP=35;DP4=1,2,3,4;AN=2;AC=1 GT:GQ:DP:GL 0/1:409:35:-20,-5,-20 1 3062915 . G T 1806 q10 DP=35;DP4=1,2,3,4;AN=2;AC=1 GT:GQ:DP:GL 0/1:409:35:-20,-5,-20 1 3106154 . CA C 1792 PASS DP=32;AN=2;AC=1 GT:GQ:DP 0/1:245:32 1 3106154 . C T,CT 1792 PASS DP=32;AN=2;AC=1 GT:GQ:DP 0/1:245:32 1 3157410 . G A 628 q10 DP=21;AN=2;AC=2 GT:GQ:DP 1/1:21:21 1 3162006 . G A 1016 PASS DP=22;AN=2;AC=1 GT:GQ:DP 0/1:212:22 1 3177144 . GT G 727 PASS DP=30;AN=2;AC=1 GT:GQ:DP 0/1:150:30 1 3184885 . T TA 246 PASS DP=10;AN=2;AC=1,1 GT:GQ:DP 1/2:12:10 2 3199812 . G T 481 PASS DP=26;AN=2;AC=1,1 GT:GQ:DP 1/2:322:26 2 3199815 . C T 481 PASS DP=26;AN=2;AC=1,1 GT:GQ:DP 1/2:322:26 3 3212016 . C A 565 PASS DP=26;AN=2;AC=1,1 GT:GQ:DP 1/2:91:26 3 3212026 . C A 565 PASS DP=26;AN=2;AC=1,1 GT:GQ:DP 1/2:91:26 3 3212036 . C A 565 PASS DP=26;AN=2;AC=1,1 GT:GQ:DP 1/2:91:26 bcftools-1.2/test/reheader.1.out000066400000000000000000000050331246371514100166220ustar00rootroot00000000000000##fileformat=VCFv4.2 ##FILTER= ##INFO= ##FILTER= ##FORMAT= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##contig= ##contig= ##contig= ##contig= ##contig= ##contig= ##contig= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT AAA0001 BBB0002 2 101 . ATTTTTTTTTTTTT ATTTTTTTTTTTTTTT 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 2 114 . TC TTCC,TTC 999 PASS INDEL;AN=4;AC=2,2 GT:DP 1/2:1 1/2:1 2 115 . C T 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 20 3 . G CT 999 PASS INDEL;AN=4;AC=2 GT 0/1 0/1 20 3 . GATG GACT 999 PASS INDEL;AN=4;AC=2 GT 1/0 1/0 20 5 . TGGG TAC,TG,TGGGG,AC . PASS INDEL;AN=4;AC=2,2,0,0 GT:PL:DP 1/2:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15:1 1/2:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15:1 20 59 . AG . 999 PASS AN=4 GT:PL:DP 0/0:0:4 0/0:0:4 20 80 . CACAG CACAT 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 81 . A C 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG ACACCG 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG AAAAAA 999 Test AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 273 . CAAAAAAAAAAAAAAAAAAAAA CAAAAAAAAAAAAAAAAAAAAAAA,CAAAAAAAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=4;AC=2,2 GT:PL:DP 1/2:0,3,5,3,5,5:1 1/2:0,3,5,3,5,5:1 20 274 . AAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 278 . AAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 3 10 . GTGGAC GTGGACACAC,GTGGACAC,GTGGACACACAC,GTGG,GTGGACACACACAC,ATGGACACACAC 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 3 15 . CACA CAC 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 4 21 . ATTTTTTTTTTTTTTTC ATTTTTTTTTTTTTTC,ATTTTTTTTTTTTTTTT,ATTTTTTTTTTTTTTTTC 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 5 22 . A AGA 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 bcftools-1.2/test/reheader.1.out.bcf000066400000000000000000000050331246371514100173530ustar00rootroot00000000000000##fileformat=VCFv4.2 ##FILTER= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##contig= ##contig= ##contig= ##contig= ##contig= ##contig= ##FILTER= ##INFO= ##FORMAT= ##contig= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT AAA0001 BBB0002 2 101 . ATTTTTTTTTTTTT ATTTTTTTTTTTTTTT 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 2 114 . TC TTCC,TTC 999 PASS INDEL;AN=4;AC=2,2 GT:DP 1/2:1 1/2:1 2 115 . C T 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 20 3 . G CT 999 PASS INDEL;AN=4;AC=2 GT 0/1 0/1 20 3 . GATG GACT 999 PASS INDEL;AN=4;AC=2 GT 1/0 1/0 20 5 . TGGG TAC,TG,TGGGG,AC . PASS INDEL;AN=4;AC=2,2,0,0 GT:PL:DP 1/2:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15:1 1/2:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15:1 20 59 . AG . 999 PASS AN=4 GT:PL:DP 0/0:0:4 0/0:0:4 20 80 . CACAG CACAT 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 81 . A C 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG ACACCG 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG AAAAAA 999 Test AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 273 . CAAAAAAAAAAAAAAAAAAAAA CAAAAAAAAAAAAAAAAAAAAAAA,CAAAAAAAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=4;AC=2,2 GT:PL:DP 1/2:0,3,5,3,5,5:1 1/2:0,3,5,3,5,5:1 20 274 . AAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 278 . AAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 3 10 . GTGGAC GTGGACACAC,GTGGACAC,GTGGACACACAC,GTGG,GTGGACACACACAC,ATGGACACACAC 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 3 15 . CACA CAC 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 4 21 . ATTTTTTTTTTTTTTTC ATTTTTTTTTTTTTTC,ATTTTTTTTTTTTTTTT,ATTTTTTTTTTTTTTTTC 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 5 22 . A AGA 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 bcftools-1.2/test/reheader.2.out000066400000000000000000000075711246371514100166340ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##contig= ##contig= ##contig= ##contig= ##contig= ##contig= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT AAA BBB 2 101 . ATTTTTTTTTTTTT ATTTTTTTTTTTTTTT 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 2 114 . TC TTCC,TTC 999 PASS INDEL;AN=4;AC=2,2 GT:DP 1/2:1 1/2:1 2 115 . C T 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 20 3 . G CT 999 PASS INDEL;AN=4;AC=2 GT 0/1 0/1 20 3 . GATG GACT 999 PASS INDEL;AN=4;AC=2 GT 1/0 1/0 20 5 . TGGG TAC,TG,TGGGG,AC . PASS INDEL;AN=4;AC=2,2,0,0 GT:PL:DP 1/2:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15:1 1/2:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15:1 20 59 . AG . 999 PASS AN=4 GT:PL:DP 0/0:0:4 0/0:0:4 20 80 . CACAG CACAT 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 81 . A C 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG ACACCG 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG AAAAAA 999 Test AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 273 . CAAAAAAAAAAAAAAAAAAAAA CAAAAAAAAAAAAAAAAAAAAAAA,CAAAAAAAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=4;AC=2,2 GT:PL:DP 1/2:0,3,5,3,5,5:1 1/2:0,3,5,3,5,5:1 20 274 . AAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 278 . AAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 3 10 . GTGGAC GTGGACACAC,GTGGACAC,GTGGACACACAC,GTGG,GTGGACACACACAC,ATGGACACACAC 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 3 15 . CACA CAC 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 4 21 . ATTTTTTTTTTTTTTTC ATTTTTTTTTTTTTTC,ATTTTTTTTTTTTTTTT,ATTTTTTTTTTTTTTTTC 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 5 22 . A AGA 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 bcftools-1.2/test/reheader.hdr000066400000000000000000000017601246371514100164340ustar00rootroot00000000000000##fileformat=VCFv4.2 ##INFO= ##FILTER= ##FORMAT= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##contig= ##contig= ##contig= ##contig= ##contig= ##contig= ##contig= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT AAA0001 BBB0002 bcftools-1.2/test/reheader.samples000066400000000000000000000000101246371514100173060ustar00rootroot00000000000000AAA BBB bcftools-1.2/test/reheader.samples2000066400000000000000000000000301246371514100173720ustar00rootroot00000000000000XY00002 BBB XY00001 AAA bcftools-1.2/test/reheader.vcf000066400000000000000000000075151246371514100164410ustar00rootroot00000000000000##fileformat=VCFv4.1 ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##contig= ##contig= ##contig= ##contig= ##contig= ##contig= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT XY00001 XY00002 2 101 . ATTTTTTTTTTTTT ATTTTTTTTTTTTTTT 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 2 114 . TC TTCC,TTC 999 PASS INDEL;AN=4;AC=2,2 GT:DP 1/2:1 1/2:1 2 115 . C T 999 PASS INDEL;AN=4;AC=4 GT:DP 1/1:1 1/1:1 20 3 . G CT 999 PASS INDEL;AN=4;AC=2 GT 0/1 0/1 20 3 . GATG GACT 999 PASS INDEL;AN=4;AC=2 GT 1/0 1/0 20 5 . TGGG TAC,TG,TGGGG,AC . PASS INDEL;AN=4;AC=2,2,0,0 GT:PL:DP 1/2:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15:1 1/2:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15:1 20 59 . AG . 999 PASS AN=4 GT:PL:DP 0/0:0:4 0/0:0:4 20 80 . CACAG CACAT 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 81 . A C 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG ACACCG 999 PASS AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 95 . TCACCG AAAAAA 999 Test AN=4;AC=2 GT:PL:DP 0/1:255,0,255:13 0/1:255,0,255:13 20 273 . CAAAAAAAAAAAAAAAAAAAAA CAAAAAAAAAAAAAAAAAAAAAAA,CAAAAAAAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=4;AC=2,2 GT:PL:DP 1/2:0,3,5,3,5,5:1 1/2:0,3,5,3,5,5:1 20 274 . AAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 20 278 . AAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA 999 PASS INDEL;AN=0;AC=0 GT:PL:DP ./.:0,0,0:0 ./.:0,0,0:0 3 10 . GTGGAC GTGGACACAC,GTGGACAC,GTGGACACACAC,GTGG,GTGGACACACACAC,ATGGACACACAC 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 3 15 . CACA CAC 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 4 21 . ATTTTTTTTTTTTTTTC ATTTTTTTTTTTTTTC,ATTTTTTTTTTTTTTTT,ATTTTTTTTTTTTTTTTC 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 5 22 . A AGA 999 PASS INDEL;AN=0 GT:DP ./.:0 ./.:0 bcftools-1.2/test/stats.a.vcf000066400000000000000000000005731246371514100162340ustar00rootroot00000000000000##fileformat=VCFv4.2 ##FORMAT= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B C 1 1000 . G A . PASS . GT 0/0 0/1 1/1 1 1001 . G A . PASS . GT 0/0 0/1 1/1 1 1002 . G A . PASS . GT 0/0 0/1 1/1 bcftools-1.2/test/stats.b.vcf000066400000000000000000000005731246371514100162350ustar00rootroot00000000000000##fileformat=VCFv4.2 ##FORMAT= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B C 1 1000 . G A . PASS . GT 0/1 0/0 0/0 1 1001 . G A . PASS . GT 0/0 0/0 0/0 1 1002 . G A . PASS . GT 0/0 0/1 0/0 bcftools-1.2/test/stats.chk000066400000000000000000000034611246371514100160030ustar00rootroot00000000000000SN 0 number of samples: 3 SN 1 number of samples: 3 SN 0 number of records: 0 SN 0 number of SNPs: 0 SN 0 number of MNPs: 0 SN 0 number of indels: 0 SN 0 number of others: 0 SN 0 number of multiallelic sites: 0 SN 0 number of multiallelic SNP sites: 0 SN 1 number of records: 0 SN 1 number of SNPs: 0 SN 1 number of MNPs: 0 SN 1 number of indels: 0 SN 1 number of others: 0 SN 1 number of multiallelic sites: 0 SN 1 number of multiallelic SNP sites: 0 SN 2 number of records: 3 SN 2 number of SNPs: 3 SN 2 number of MNPs: 0 SN 2 number of indels: 0 SN 2 number of others: 0 SN 2 number of multiallelic sites: 0 SN 2 number of multiallelic SNP sites: 0 TSTV 0 0 0 0.00 0 0 0.00 TSTV 1 0 0 0.00 0 0 0.00 TSTV 2 3 0 0.00 3 0 0.00 SiS 0 1 0 0 0 0 0 0 0 SiS 1 1 0 0 0 0 0 0 0 SiS 2 1 0 0 0 0 0 0 0 AF 2 49.000000 3 3 0 0 0 0 0 QUAL 2 998 3 3 0 0 ST 0 A>C 0 ST 0 A>G 0 ST 0 A>T 0 ST 0 C>A 0 ST 0 C>G 0 ST 0 C>T 0 ST 0 G>A 0 ST 0 G>C 0 ST 0 G>T 0 ST 0 T>A 0 ST 0 T>C 0 ST 0 T>G 0 ST 1 A>C 0 ST 1 A>G 0 ST 1 A>T 0 ST 1 C>A 0 ST 1 C>G 0 ST 1 C>T 0 ST 1 G>A 0 ST 1 G>C 0 ST 1 G>T 0 ST 1 T>A 0 ST 1 T>C 0 ST 1 T>G 0 ST 2 A>C 0 ST 2 A>G 0 ST 2 A>T 0 ST 2 C>A 0 ST 2 C>G 0 ST 2 C>T 0 ST 2 G>A 3 ST 2 G>C 0 ST 2 G>T 0 ST 2 T>A 0 ST 2 T>C 0 ST 2 T>G 0 SN 2 number of samples: 3 GCsAF 2 49.000000 2 1 0 1 2 3 0.375000 2 NRDs 2 85.714286 33.333333 66.666667 100.000000 NRDi 2 0.000000 0.000000 0.000000 0.000000 GCsS 2 A 100.000 2 0 0 1 0 0 GCsS 2 B 66.667 0 1 0 0 2 0 GCsS 2 C 100.000 0 0 0 0 0 3 GCiS 2 A 0.000 0 0 0 0 0 0 GCiS 2 B 0.000 0 0 0 0 0 0 GCiS 2 C 0.000 0 0 0 0 0 0 PSC 0 A 0 0 0 0 0 0 0.0 0 PSC 0 B 0 0 0 0 0 0 0.0 0 PSC 0 C 0 0 0 0 0 0 0.0 0 PSC 1 A 0 0 0 0 0 0 0.0 0 PSC 1 B 0 0 0 0 0 0 0.0 0 PSC 1 C 0 0 0 0 0 0 0.0 0 PSC 2 A 3 0 0 0 0 0 0.0 0 PSC 2 B 0 0 3 3 0 0 0.0 0 PSC 2 C 0 3 0 3 0 0 0.0 0 HWE 2 49.000000 3 0.330000 0.330000 0.330000 bcftools-1.2/test/tabix.1.3000151.out000066400000000000000000000001031246371514100167530ustar00rootroot000000000000001 3000151 . C T 59.2 PASS AN=4;AC=2 GT:DP:GQ 0/1:32:245 0/1:32:245 bcftools-1.2/test/tabix.2.3199812.out000066400000000000000000000001121246371514100170030ustar00rootroot000000000000002 3199812 . G GTT,GT 82.7 PASS AN=4;AC=2,2 GT:GQ:DP 1/2:322:26 1/2:322:26 bcftools-1.2/test/test-rbuf.c000066400000000000000000000042141246371514100162320ustar00rootroot00000000000000/* test/test-rbuf.c -- rbuf_t test harness. Copyright (C) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include "rbuf.h" void debug_print(rbuf_t *rbuf, int *dat) { int i; for (i=-1; rbuf_next(rbuf, &i); ) printf(" %2d", i); printf("\n"); for (i=-1; rbuf_next(rbuf, &i); ) printf(" %2d", dat[i]); printf("\n"); } int main(int argc, char **argv) { int i, j, *dat = (int*)calloc(10,sizeof(int)); rbuf_t rbuf; rbuf_init(&rbuf,10); rbuf.f = 5; // force wrapping for (i=0; i<9; i++) { j = rbuf_append(&rbuf); dat[j] = i+1; } printf("Inserted 1-9 starting at offset 5:\n"); debug_print(&rbuf, dat); i = rbuf_kth(&rbuf, 3); printf("4th is %d\n", dat[i]); printf("Deleting 1-2:\n"); rbuf_shift_n(&rbuf, 2); debug_print(&rbuf, dat); printf("Prepending 0-8:\n"); for (i=0; i<9; i++) { j = rbuf_prepend(&rbuf); dat[j] = i; } debug_print(&rbuf, dat); printf("Expanding:\n"); rbuf_expand0(&rbuf,int,dat); debug_print(&rbuf, dat); free(dat); return 0; } bcftools-1.2/test/test.pl000077500000000000000000001206231246371514100154750ustar00rootroot00000000000000#!/usr/bin/env perl # # Copyright (C) 2012-2014 Genome Research Ltd. # # Author: Petr Danecek # # Permission is hereby granted, free of charge, to any person obtaining a copy # of this software and associated documentation files (the "Software"), to deal # in the Software without restriction, including without limitation the rights # to use, copy, modify, merge, publish, distribute, sublicense, and/or sell # copies of the Software, and to permit persons to whom the Software is # furnished to do so, subject to the following conditions: # # The above copyright notice and this permission notice shall be included in # all copies or substantial portions of the Software. # # THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR # IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, # FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL # THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER # LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING # FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER # DEALINGS IN THE SOFTWARE. use strict; use warnings; use Carp; #use IPC::Open2; use FindBin; use lib "$FindBin::Bin"; use Getopt::Long; use File::Temp qw/ tempfile tempdir /; my $opts = parse_params(); test_usage($opts,cmd=>'bcftools'); test_tabix($opts,in=>'merge.a',reg=>'2:3199812-3199812',out=>'tabix.2.3199812.out'); test_tabix($opts,in=>'merge.a',reg=>'1:3000151-3000151',out=>'tabix.1.3000151.out'); test_tabix($opts,in=>'large_chrom_tbi_limit',reg=>'chr11:1-536870912',out=>'large_chrom_tbi_limit.20.1.536870912.out'); # 536870912 (1<<29) is the current limit for tbi. cannot retrieve regions larger than that test_index($opts,in=>'large_chrom_csi_limit',reg=>'chr20:1-2147483647',out=>'large_chrom_csi_limit.20.1.2147483647.out'); # 2147483647 (1<<31-1) is the current chrom limit for csi. bcf conversion and indexing fail above this test_index($opts,in=>'large_chrom_csi_limit',reg=>'chr20',out=>'large_chrom.20.1.2147483647.out'); # this fails until bug resolved test_vcf_idxstats($opts,in=>'idx',args=>'-s',out=>'idx.out'); test_vcf_idxstats($opts,in=>'idx',args=>'-n',out=>'idx_count.out'); test_vcf_idxstats($opts,in=>'empty',args=>'-s',out=>'empty.idx.out'); test_vcf_idxstats($opts,in=>'empty',args=>'-n',out=>'empty.idx_count.out'); test_vcf_check($opts,in=>'check',out=>'check.chk'); test_vcf_stats($opts,in=>['stats.a','stats.b'],out=>'stats.chk',args=>'-s -'); test_vcf_isec($opts,in=>['isec.a','isec.b'],out=>'isec.ab.out',args=>'-n =2'); test_vcf_isec($opts,in=>['isec.a','isec.b'],out=>'isec.ab.flt.out',args=>'-n =2 -i"STRLEN(REF)==2"'); test_vcf_isec($opts,in=>['isec.a','isec.b'],out=>'isec.ab.both.out',args=>'-n =2 -c both'); test_vcf_isec($opts,in=>['isec.a','isec.b'],out=>'isec.ab.any.out',args=>'-n =2 -c any'); test_vcf_isec($opts,in=>['isec.a','isec.b'],out=>'isec.ab.C.out',args=>'-C -c any'); test_vcf_isec2($opts,vcf_in=>['isec.a'],tab_in=>'isec',out=>'isec.tab.out',args=>''); test_vcf_merge($opts,in=>['merge.a','merge.b','merge.c'],out=>'merge.abc.out',args=>'--force-samples'); test_vcf_merge($opts,in=>['merge.2.a','merge.2.b'],out=>'merge.2.none.out',args=>'--force-samples -m none'); test_vcf_merge($opts,in=>['merge.2.a','merge.2.b'],out=>'merge.2.both.out',args=>'--force-samples -m both'); test_vcf_merge($opts,in=>['merge.2.a','merge.2.b'],out=>'merge.2.all.out',args=>'--force-samples -m all'); test_vcf_merge($opts,in=>['merge.3.a','merge.3.b'],out=>'merge.3.out',args=>'--force-samples -i TR:sum,TA:sum,TG:sum'); test_vcf_merge($opts,in=>['merge.4.a','merge.4.b'],out=>'merge.4.out',args=>'--force-samples -m id'); test_vcf_query($opts,in=>'query',out=>'query.out',args=>q[-f '%CHROM\\t%POS\\t%REF\\t%ALT\\t%DP4\\t%AN[\\t%GT\\t%TGT]\\n']); test_vcf_query($opts,in=>'view.filter',out=>'query.2.out',args=>q[-f'%XRI\\n' -i'XRI[*]>1111']); test_vcf_query($opts,in=>'view.filter',out=>'query.3.out',args=>q[-f'%XRF\\n' -i'XRF[*]=2e6']); test_vcf_query($opts,in=>'view.filter',out=>'query.4.out',args=>q[-f'%XGS\\n' -i'XGS[5]="PQR"']); test_vcf_query($opts,in=>'view.filter',out=>'query.4.out',args=>q[-f'%XGS\\n' -i'XGS[*]="GHI"']); test_vcf_query($opts,in=>'view.filter',out=>'query.4.out',args=>q[-f'%XGS\\n' -i'XGS[2]~"H"']); test_vcf_query($opts,in=>'view.filter',out=>'query.4.out',args=>q[-f'%XGS\\n' -i'XGS[3]!~"H"']); test_vcf_query($opts,in=>'view.filter',out=>'query.4.out',args=>q[-f'%XGS\\n' -i'XGS[*]~"H"']); test_vcf_query($opts,in=>'view.filter',out=>'query.4.out',args=>q[-f'%XGS\\n' -i'XGS[*]~"HI,JK"']); test_vcf_query($opts,in=>'query',out=>'query.5.out',args=>q[-f'%POS %REF %ALT\\n' -i'REF~"C" && ALT~"CT"']); test_vcf_query($opts,in=>'query',out=>'query.6.out',args=>q[-f'%POS %REF %ALT\\n' -i'N_ALT=2']); test_vcf_query($opts,in=>'query',out=>'query.7.out',args=>q[-f'%POS %AN\\n' -i'AN!=2*N_SAMPLES']); test_vcf_query($opts,in=>'query',out=>'query.8.out',args=>q[-f'%POS[ %GL]\\n' -i'min(abs(GL[0]))=10']); test_vcf_query($opts,in=>'view.filter',out=>'query.9.out',args=>q[-f'%POS %CIGAR\\n' -i'strlen(CIGAR[*])=4']); test_vcf_query($opts,in=>'query',out=>'query.10.out',args=>q[-f'%POS[ %GT]\\n' -i'AC[0]=3']); test_vcf_query($opts,in=>'query',out=>'query.10.out',args=>q[-f'%POS[ %GT]\\n' -i'AF[0]=3/4']); test_vcf_query($opts,in=>'query',out=>'query.11.out',args=>q[-f'%POS[ %GT]\\n' -i'MAC[0]=1']); test_vcf_query($opts,in=>'query',out=>'query.11.out',args=>q[-f'%POS[ %GT]\\n' -i'MAF[0]=1/4']); test_vcf_query($opts,in=>'view.vectors',out=>'query.12.out',args=>q[-f'I8=%I8 I16=%I16 I32=%I32 IF=%IF IA8=%IA8 IA16=%IA16 IA32=%IA32 IAF=%IAF IA8=%IA8{1} IA16=%IA16{1} IA32=%IA32{1} IAF=%IAF{1} [ %F8:%F16:%F32:%FF]\\n']); test_vcf_query($opts,in=>'query.filter',out=>'query.13.out',args=>q[-f'%POS[ %GT]\\n' -i'GT ="1"']); test_vcf_query($opts,in=>'query.filter',out=>'query.14.out',args=>q[-f'%POS[ %GT]\\n' -i'GT!="1"']); test_vcf_query($opts,in=>'query.filter',out=>'query.15.out',args=>q[-f'%POS[ %GT]\\n' -e'GT ="1"']); test_vcf_query($opts,in=>'query.filter',out=>'query.16.out',args=>q[-f'%POS[ %GT]\\n' -e'GT!="1"']); test_vcf_query($opts,in=>'query.2',out=>'query.17.out',args=>q[-f'%XX_A %XX.A %XX.A0 %xx.a0\\n']); test_vcf_norm($opts,in=>'norm',out=>'norm.out',fai=>'norm'); test_vcf_norm($opts,in=>'norm.split',out=>'norm.split.out',args=>'-m-'); test_vcf_norm($opts,in=>'norm.merge',out=>'norm.merge.out',args=>'-m+'); test_vcf_norm($opts,in=>'norm.merge',out=>'norm.merge.strict.out',args=>'-m+ -s'); test_vcf_view($opts,in=>'view',out=>'view.1.out',args=>'-aUc1 -C1 -s NA00002 -v snps',reg=>''); test_vcf_view($opts,in=>'view',out=>'view.2.out',args=>'-f PASS -Xks NA00003',reg=>'-r20,Y'); test_vcf_view($opts,in=>'view',out=>'view.3.out',args=>'-xs NA00003',reg=>''); test_vcf_view($opts,in=>'view',out=>'view.4.out',args=>q[-i 'QUAL==999 && (FS<20 || FS>=41.02) && ICF>-0.1 && HWE*2>1.2'],reg=>''); test_vcf_view($opts,in=>'view',out=>'view.5.out',args=>q[-p],reg=>''); test_vcf_view($opts,in=>'view',out=>'view.6.out',args=>q[-P],reg=>''); test_vcf_view($opts,in=>'view',out=>'view.7.out',args=>q[-hm2 -M2 -q0.3 -Q0.7],reg=>''); test_vcf_view($opts,in=>'view',out=>'view.8.out',args=>q[-Hu],reg=>''); test_vcf_view($opts,in=>'view',out=>'view.9.out',args=>q[-GVsnps],reg=>''); test_vcf_view($opts,in=>'view',out=>'view.10.out',args=>q[-ne 'INDEL=1 || PV4[0]<0.006'],reg=>''); test_vcf_view($opts,in=>'view',out=>'view.exclude.out',args=>'-s ^NA00003',reg=>''); test_vcf_view($opts,in=>'view.omitgenotypes',out=>'view.omitgenotypes.out',args=>'',reg=>''); test_vcf_view($opts,in=>'view.vectors',out=>'view.vectors.A.out',args=>'-asA',reg=>''); test_vcf_view($opts,in=>'view.vectors',out=>'view.vectors.B.out',args=>'-asB',reg=>''); test_vcf_view($opts,in=>'view.filter',out=>'view.filter.1.out',args=>q[-H -i'FMT/FGS[0]="AAAAAA"'],reg=>''); # test expressions test_vcf_view($opts,in=>'view.filter',out=>'view.filter.2.out',args=>q[-H -i'FMT/FGS[2]="C"'],reg=>''); test_vcf_view($opts,in=>'view.filter',out=>'view.filter.3.out',args=>q[-H -i'FMT/FGS[4]="EE"'],reg=>''); test_vcf_view($opts,in=>'view.filter',out=>'view.filter.4.out',args=>q[-H -i'FMT/FRS[1]="BB"'],reg=>''); test_vcf_view($opts,in=>'view.filter',out=>'view.filter.5.out',args=>q[-H -i'TXT0="text"'],reg=>''); test_vcf_view($opts,in=>'view.chrs',out=>'view.chrs.out',args=>'',reg=>'',tgts=>'view.chrs.tab'); test_vcf_filter($opts,in=>'view.filter',out=>'view.filter.6.out',args=>q[-S. -e'TXT0="text"'],reg=>''); test_vcf_filter($opts,in=>'view.filter',out=>'view.filter.7.out',args=>q[-S. -e'FMT/FRS[1]="BB"'],reg=>''); test_vcf_filter($opts,in=>'view.filter',out=>'view.filter.8.out',args=>q[-S. -e'FMT/FGS[0]="AAAAAA"'],reg=>''); test_vcf_filter($opts,in=>'view.filter',out=>'view.filter.9.out',args=>q[-S. -e'FMT/FGS[1]="BBB"'],reg=>''); test_vcf_filter($opts,in=>'view.filter',out=>'view.filter.10.out',args=>q[-S. -e'FMT/FGS[4]="EE"'],reg=>''); test_vcf_filter($opts,in=>'view.filter',out=>'view.filter.11.out',args=>q[-S. -e'FMT/STR="XX"'],reg=>''); test_vcf_view($opts,in=>'view.minmaxac',out=>'view.minmaxac.1.out',args=>q[-H -C5:nonmajor],reg=>''); test_vcf_view($opts,in=>'view.minmaxac',out=>'view.minmaxac.2.out',args=>q[-H -c6:nonmajor],reg=>''); test_vcf_view($opts,in=>'view.minmaxac',out=>'view.minmaxac.1.out',args=>q[-H -q0.3:major],reg=>''); test_vcf_call($opts,in=>'mpileup',out=>'mpileup.1.out',args=>'-mv'); test_vcf_call($opts,in=>'mpileup',out=>'mpileup.2.out',args=>'-mvg0'); test_vcf_call_cAls($opts,in=>'mpileup',out=>'mpileup.cAls.out',tab=>'mpileup'); test_vcf_filter($opts,in=>'filter.1',out=>'filter.1.out',args=>'-mx -g2 -G2'); test_vcf_filter($opts,in=>'filter.2',out=>'filter.2.out',args=>q[-e'QUAL==59.2 || (INDEL=0 & (FMT/GQ=25 | FMT/DP=10))' -sModified -S.]); test_vcf_filter($opts,in=>'filter.3',out=>'filter.3.out',args=>q[-e'DP=19'],fmt=>'%POS\\t%FILTER\\t%DP[\\t%GT]\\n'); test_vcf_filter($opts,in=>'filter.3',out=>'filter.4.out',args=>q[-e'DP=19' -s XX],fmt=>'%POS\\t%FILTER\\t%DP[\\t%GT]\\n'); test_vcf_filter($opts,in=>'filter.3',out=>'filter.5.out',args=>q[-e'DP=19' -s XX -m+],fmt=>'%POS\\t%FILTER\\t%DP[\\t%GT]\\n'); test_vcf_filter($opts,in=>'filter.3',out=>'filter.6.out',args=>q[-e'DP=19' -s XX -mx],fmt=>'%POS\\t%FILTER\\t%DP[\\t%GT]\\n'); test_vcf_filter($opts,in=>'filter.3',out=>'filter.7.out',args=>q[-e'DP=19' -s XX -m+x],fmt=>'%POS\\t%FILTER\\t%DP[\\t%GT]\\n'); test_vcf_filter($opts,in=>'filter.3',out=>'filter.3.out',args=>q[-e'FMT/GT="0/2"'],fmt=>'%POS\\t%FILTER\\t%DP[\\t%GT]\\n'); test_vcf_filter($opts,in=>'filter.3',out=>'filter.4.out',args=>q[-e'FMT/GT="0/2"' -s XX],fmt=>'%POS\\t%FILTER\\t%DP[\\t%GT]\\n'); test_vcf_filter($opts,in=>'filter.3',out=>'filter.5.out',args=>q[-e'FMT/GT="0/2"' -s XX -m+],fmt=>'%POS\\t%FILTER\\t%DP[\\t%GT]\\n'); test_vcf_filter($opts,in=>'filter.3',out=>'filter.6.out',args=>q[-e'FMT/GT="0/2"' -s XX -mx],fmt=>'%POS\\t%FILTER\\t%DP[\\t%GT]\\n'); test_vcf_filter($opts,in=>'filter.3',out=>'filter.7.out',args=>q[-e'FMT/GT="0/2"' -s XX -m+x],fmt=>'%POS\\t%FILTER\\t%DP[\\t%GT]\\n'); test_vcf_filter($opts,in=>'filter.2',out=>'filter.8.out',args=>q[-i'FMT/GT="0/0" && AC[*]=2'],fmt=>'%POS\\t%AC[\\t%GT]\\n'); test_vcf_filter($opts,in=>'filter.2',out=>'filter.8.out',args=>q[-i'AC[*]=2 && FMT/GT="0/0"'],fmt=>'%POS\\t%AC[\\t%GT]\\n'); test_vcf_regions($opts,in=>'regions'); test_vcf_annotate($opts,in=>'annotate',tab=>'annotate',out=>'annotate.out',args=>'-c CHROM,POS,REF,ALT,ID,QUAL,INFO/T_INT,INFO/T_FLOAT,INDEL'); test_vcf_annotate($opts,in=>'annotate',tab=>'annotate2',out=>'annotate2.out',args=>'-c CHROM,FROM,TO,T_STR'); test_vcf_annotate($opts,in=>'annotate',vcf=>'annots',out=>'annotate3.out',args=>'-c STR,ID,QUAL,FILTER'); test_vcf_annotate($opts,in=>'annotate2',vcf=>'annots2',out=>'annotate4.out',args=>'-c ID,QUAL,FILTER,INFO,FMT'); test_vcf_annotate($opts,in=>'annotate2',vcf=>'annots2',out=>'annotate5.out',args=>'-c ID,QUAL,+FILTER,+INFO,FMT/GT -s A'); test_vcf_annotate($opts,in=>'annotate3',out=>'annotate6.out',args=>'-x ID,QUAL,^FILTER/fltA,FILTER/fltB,^INFO/AA,INFO/BB,^FMT/GT,FMT/PL'); test_vcf_annotate($opts,in=>'annotate3',out=>'annotate7.out',args=>'-x FORMAT'); test_vcf_annotate($opts,in=>'annotate4',vcf=>'annots4',out=>'annotate8.out',args=>'-c +INFO'); test_vcf_annotate($opts,in=>'annotate4',tab=>'annots4',out=>'annotate8.out',args=>'-c CHROM,POS,REF,ALT,+FA,+FR,+IA,+IR,+SA,+SR'); test_vcf_plugin($opts,in=>'plugin1',out=>'missing2ref.out',cmd=>'+missing2ref'); test_vcf_plugin($opts,in=>'plugin1',out=>'fill-AN-AC.out',cmd=>'+fill-AN-AC'); test_vcf_plugin($opts,in=>'plugin1',out=>'dosage.out',cmd=>'+dosage'); test_vcf_plugin($opts,in=>'fixploidy',out=>'fixploidy.out',cmd=>'+fixploidy',args=>'-- -s {PATH}/fixploidy.samples -p {PATH}/fixploidy.ploidy'); test_vcf_plugin($opts,in=>'vcf2sex',out=>'vcf2sex.out',cmd=>'+vcf2sex',args=>'-- -n 5'); test_vcf_plugin($opts,in=>'vcf2sex',out=>'vcf2sex.out',cmd=>'+vcf2sex',args=>'-- -gn 5'); test_vcf_concat($opts,in=>['concat.1.a','concat.1.b'],out=>'concat.1.vcf.out',do_bcf=>0,args=>''); test_vcf_concat($opts,in=>['concat.1.a','concat.1.b'],out=>'concat.1.bcf.out',do_bcf=>1,args=>''); test_vcf_concat($opts,in=>['concat.2.a','concat.2.b'],out=>'concat.2.vcf.out',do_bcf=>0,args=>'-a'); test_vcf_concat($opts,in=>['concat.2.a','concat.2.b'],out=>'concat.2.bcf.out',do_bcf=>1,args=>'-a'); test_vcf_concat($opts,in=>['concat.2.a','concat.2.b'],out=>'concat.4.vcf.out',do_bcf=>0,args=>'-aD'); test_vcf_concat($opts,in=>['concat.2.a','concat.2.b'],out=>'concat.4.bcf.out',do_bcf=>1,args=>'-aD'); test_vcf_concat($opts,in=>['concat.3.a','concat.3.b','concat.3.0','concat.3.c','concat.3.d','concat.3.e','concat.3.f'],out=>'concat.3.vcf.out',do_bcf=>0,args=>'-l'); test_vcf_concat($opts,in=>['concat.3.a','concat.3.b','concat.3.0','concat.3.c','concat.3.d','concat.3.e','concat.3.f'],out=>'concat.3.bcf.out',do_bcf=>1,args=>'-l'); test_vcf_reheader($opts,in=>'reheader',out=>'reheader.1.out',header=>'reheader.hdr'); test_vcf_reheader($opts,in=>'reheader',out=>'reheader.2.out',samples=>'reheader.samples'); test_vcf_reheader($opts,in=>'reheader',out=>'reheader.2.out',samples=>'reheader.samples2'); test_rename_chrs($opts,in=>'annotate'); test_vcf_convert($opts,in=>'convert',out=>'convert.gs.gt.gen',args=>'-g -,.'); test_vcf_convert($opts,in=>'convert',out=>'convert.gs.gt.samples',args=>'-g .,-'); test_vcf_convert($opts,in=>'convert',out=>'convert.gs.pl.gen',args=>'-g -,. --tag PL'); test_vcf_convert($opts,in=>'convert',out=>'convert.gs.pl.samples',args=>'-g .,- --tag PL'); test_vcf_convert($opts,in=>'check',out=>'check.gs.vcfids.gen',args=>'-g -,. --vcf-ids'); test_vcf_convert($opts,in=>'check',out=>'check.gs.vcfids.samples',args=>'-g .,- --vcf-ids'); test_vcf_convert($opts,in=>'check',out=>'check.gs.chrom.gen',args=>'-g -,. --chrom'); test_vcf_convert($opts,in=>'check',out=>'check.gs.chrom.samples',args=>'-g .,- --chrom'); test_vcf_convert($opts,in=>'check',out=>'check.gs.vcfids_chrom.gen',args=>'-g -,. --chrom --vcf-ids'); test_vcf_convert($opts,in=>'check',out=>'check.gs.vcfids_chrom.samples',args=>'-g .,- --chrom --vcf-ids'); test_vcf_convert($opts,in=>'convert',out=>'convert.hls.haps',args=>'-h -,.,.'); test_vcf_convert($opts,in=>'convert',out=>'convert.hls.legend',args=>'-h .,-,.'); test_vcf_convert($opts,in=>'convert',out=>'convert.hls.samples',args=>'-h .,.,-'); test_vcf_convert($opts,in=>'convert',out=>'convert.hs.hap',args=>'--hapsample -,.'); test_vcf_convert($opts,in=>'convert',out=>'convert.hs.sample',args=>'--hapsample .,-'); test_vcf_convert_gvcf($opts,in=>'convert.gvcf',out=>'convert.gvcf.out',args=>'--gvcf2vcf'); test_vcf_convert_tsv2vcf($opts,in=>'convert.23andme',out=>'convert.23andme.vcf',args=>'-c ID,CHROM,POS,AA -s SAMPLE1',fai=>'23andme'); test_vcf_consensus($opts,in=>'consensus',out=>'consensus.1.out',fa=>'consensus.fa',mask=>'consensus.tab',args=>''); test_vcf_consensus_chain($opts,in=>'consensus',out=>'consensus.1.chain',chain=>'consensus.1.chain',fa=>'consensus.fa',mask=>'consensus.tab',args=>''); test_vcf_consensus($opts,in=>'consensus',out=>'consensus.2.out',fa=>'consensus.fa',mask=>'consensus.tab',args=>'-H 1'); test_vcf_consensus_chain($opts,in=>'consensus',out=>'consensus.2.chain',chain=>'consensus.2.chain',fa=>'consensus.fa',mask=>'consensus.tab',args=>'-H 1'); test_vcf_consensus($opts,in=>'consensus',out=>'consensus.3.out',fa=>'consensus.fa',mask=>'consensus.tab',args=>'-i'); test_vcf_consensus_chain($opts,in=>'consensus',out=>'consensus.3.chain',chain=>'consensus.3.chain',fa=>'consensus.fa',mask=>'consensus.tab',args=>'-i'); test_vcf_consensus($opts,in=>'consensus',out=>'consensus.4.out',fa=>'consensus.fa',args=>'-H 1'); test_vcf_consensus_chain($opts,in=>'consensus',out=>'consensus.4.chain',chain=>'consensus.4.chain',fa=>'consensus.fa',args=>'-H 1'); print "\nNumber of tests:\n"; printf " total .. %d\n", $$opts{nok}+$$opts{nfailed}; printf " passed .. %d\n", $$opts{nok}; printf " failed .. %d\n", $$opts{nfailed}; print "\n"; exit ($$opts{nfailed} != 0); #-------------------- sub error { my (@msg) = @_; if ( scalar @msg ) { confess @msg; } print "About: htslib consistency test script\n", "Usage: test.pl [OPTIONS]\n", "Options:\n", " -p, --plugins Test also plugins, requires libhts.so.\n", " -r, --redo-outputs Recreate expected output files.\n", " -t, --temp-dir When given, temporary files will not be removed.\n", " -h, -?, --help This help message.\n", "\n"; exit -1; } sub parse_params { my $opts = { bgzip=>"bgzip", keep_files=>0, nok=>0, nfailed=>0, tabix=>"tabix", plugins=>0 }; my $help; Getopt::Long::Configure('bundling'); my $ret = GetOptions ( 'e|exec=s' => sub { my ($tool, $path) = split /=/, $_[1]; $$opts{$tool} = $path if $path }, 't|temp-dir:s' => \$$opts{keep_files}, 'p|plugins' => \$$opts{test_plugins}, 'r|redo-outputs' => \$$opts{redo_outputs}, 'h|?|help' => \$help ); if ( !$ret or $help ) { error(); } $$opts{tmp} = $$opts{keep_files} ? $$opts{keep_files} : tempdir(CLEANUP=>1); if ( $$opts{keep_files} ) { cmd("mkdir -p $$opts{keep_files}"); } $$opts{path} = $FindBin::RealBin; $$opts{bin} = $FindBin::RealBin; $$opts{bin} =~ s{/test/?$}{}; return $opts; } sub _cmd { my ($cmd) = @_; my $kid_io; my @out; my $pid = open($kid_io, "-|"); if ( !defined $pid ) { error("Cannot fork: $!"); } if ($pid) { # parent @out = <$kid_io>; close($kid_io); } else { # child exec('/bin/bash', '-o','pipefail','-c', $cmd) or error("Cannot execute the command [/bin/sh -o pipefail -c $cmd]: $!"); } return ($? >> 8, join('',@out)); } sub cmd { my ($cmd) = @_; my ($ret,$out) = _cmd($cmd); if ( $ret ) { error("The command failed: $cmd\n", $out); } return $out; } sub test_cmd { my ($opts,%args) = @_; if ( !exists($args{out}) ) { if ( !exists($args{in}) ) { error("FIXME: expected out or in key\n"); } $args{out} = "$args{in}.out"; } my ($package, $filename, $line, $test)=caller(1); $test =~ s/^.+:://; print "$test:\n"; print "\t$args{cmd}\n"; my ($ret,$out) = _cmd("$args{cmd}"); if ( $ret ) { failed($opts,$test,"Non-zero status $ret"); return; } if ( $$opts{redo_outputs} && -e "$$opts{path}/$args{out}" ) { rename("$$opts{path}/$args{out}","$$opts{path}/$args{out}.old"); open(my $fh,'>',"$$opts{path}/$args{out}") or error("$$opts{path}/$args{out}: $!"); print $fh $out; close($fh); my ($ret,$out) = _cmd("diff -q $$opts{path}/$args{out} $$opts{path}/$args{out}.old"); if ( !$ret && $out eq '' ) { unlink("$$opts{path}/$args{out}.old"); } else { print "\tthe expected output changed, saving:\n"; print "\t old .. $$opts{path}/$args{out}.old\n"; print "\t new .. $$opts{path}/$args{out}\n"; } } my $exp = ''; if ( open(my $fh,'<',"$$opts{path}/$args{out}") ) { my @exp = <$fh>; $exp = join('',@exp); close($fh); } elsif ( !$$opts{redo_outputs} ) { failed($opts,$test,"$$opts{path}/$args{out}: $!"); return; } if ( $exp ne $out ) { open(my $fh,'>',"$$opts{path}/$args{out}.new") or error("$$opts{path}/$args{out}.new"); print $fh $out; close($fh); if ( !-e "$$opts{path}/$args{out}" ) { rename("$$opts{path}/$args{out}.new","$$opts{path}/$args{out}") or error("rename $$opts{path}/$args{out}.new $$opts{path}/$args{out}: $!"); print "\tthe file with expected output does not exist, creating new one:\n"; print "\t\t$$opts{path}/$args{out}\n"; } else { failed($opts,$test,"The outputs differ:\n\t\t$$opts{path}/$args{out}\n\t\t$$opts{path}/$args{out}.new"); } return; } passed($opts,$test); } sub failed { my ($opts,$test,$reason) = @_; $$opts{nfailed}++; if ( defined $reason ) { print "\n\t$reason"; } print "\n.. failed ...\n\n"; } sub passed { my ($opts,$test) = @_; $$opts{nok}++; print ".. ok\n\n"; } sub is_file_newer { my ($afile,$bfile) = @_; my (@astat) = stat($afile) or return 0; my (@bstat) = stat($bfile) or return 0; if ( $astat[9]>$bstat[9] ) { return 1 } return 0; } sub bgzip_tabix { my ($opts,%args) = @_; my $file = "$args{file}.$args{suffix}"; if ( $$opts{redo_outputs} or !-e "$$opts{tmp}/$file.gz" or is_file_newer("$$opts{path}/$file","$$opts{tmp}/$file.gz") ) { cmd("cat $$opts{path}/$file | $$opts{bgzip} -c > $$opts{tmp}/$file.gz"); } if ( $$opts{redo_outputs} or !-e "$$opts{tmp}/$file.gz.tbi" or is_file_newer("$$opts{tmp}/$file.gz","$$opts{tmp}/$file.gz.tbi") ) { cmd("$$opts{tabix} -f $args{args} $$opts{tmp}/$file.gz"); } } sub bgzip_tabix_vcf { my ($opts,$file) = @_; bgzip_tabix($opts,file=>$file,suffix=>'vcf',args=>'-p vcf'); } # The tests -------------------------- sub test_tabix { my ($opts,%args) = @_; bgzip_tabix_vcf($opts,$args{in}); test_cmd($opts,%args,cmd=>"$$opts{tabix} $$opts{tmp}/$args{in}.vcf.gz $args{reg}"); cmd("$$opts{bin}/bcftools view -Ob $$opts{tmp}/$args{in}.vcf.gz > $$opts{tmp}/$args{in}.bcf"); cmd("$$opts{bin}/bcftools index -f $$opts{tmp}/$args{in}.bcf"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools view -H $$opts{tmp}/$args{in}.bcf $args{reg}"); } sub test_index { my ($opts,%args) = @_; cmd("$$opts{bin}/bcftools view -Oz $$opts{path}/$args{in}.vcf > $$opts{tmp}/$args{in}.vcf.gz"); cmd("$$opts{bin}/bcftools index -f $$opts{tmp}/$args{in}.vcf.gz"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools view -H $$opts{tmp}/$args{in}.vcf.gz $args{reg}"); cmd("$$opts{bin}/bcftools view -Ob $$opts{path}/$args{in}.vcf > $$opts{tmp}/$args{in}.bcf"); cmd("$$opts{bin}/bcftools index -f $$opts{tmp}/$args{in}.bcf"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools view -H $$opts{tmp}/$args{in}.bcf $args{reg}"); } sub test_vcf_idxstats { my ($opts,%args) = @_; cmd("$$opts{bin}/bcftools view -Oz $$opts{path}/$args{in}.vcf > $$opts{tmp}/$args{in}.vcf.gz"); cmd("$$opts{bin}/bcftools index --tbi -f $$opts{tmp}/$args{in}.vcf.gz"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools index $args{args} $$opts{tmp}/$args{in}.vcf.gz"); cmd("$$opts{bin}/bcftools index --csi -f $$opts{tmp}/$args{in}.vcf.gz"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools index $args{args} $$opts{tmp}/$args{in}.vcf.gz"); cmd("$$opts{bin}/bcftools view -Ob $$opts{path}/$args{in}.vcf > $$opts{tmp}/$args{in}.bcf"); cmd("$$opts{bin}/bcftools index -f $$opts{tmp}/$args{in}.bcf"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools index $args{args} $$opts{tmp}/$args{in}.bcf"); } sub test_vcf_check { my ($opts,%args) = @_; bgzip_tabix_vcf($opts,$args{in}); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools stats -s - $$opts{tmp}/$args{in}.vcf.gz | grep -v '^# The command' | grep -v '^# This' | grep -v '^ID\t'"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools view -Ob $$opts{tmp}/$args{in}.vcf.gz | $$opts{bin}/bcftools stats -s - | grep -v '^# The command' | grep -v '^# This' | grep -v '^ID\t'"); } sub test_vcf_stats { my ($opts,%args) = @_; my $files = ''; for my $file (@{$args{in}}) { bgzip_tabix_vcf($opts,$file); $files .= " $$opts{tmp}/$file.vcf.gz"; } test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools stats $args{args} $files | grep -v '^#' | grep -v '^ID\t'"); } sub test_vcf_merge { my ($opts,%args) = @_; my @files; for my $file (@{$args{in}}) { bgzip_tabix_vcf($opts,$file); push @files, "$$opts{tmp}/$file.vcf.gz"; } my $args = exists($args{args}) ? $args{args} : ''; my $files = join(' ',@files); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools merge $args $files | grep -v ^##bcftools_"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools merge -Ob $args $files | $$opts{bin}/bcftools view | grep -v ^##bcftools_"); } sub test_vcf_isec { my ($opts,%args) = @_; my @files; for my $file (@{$args{in}}) { bgzip_tabix_vcf($opts,$file); push @files, "$$opts{tmp}/$file.vcf.gz"; } my $files = join(' ',@files); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools isec $args{args} $files"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools isec -Ob $args{args} $files"); } sub test_vcf_isec2 { my ($opts,%args) = @_; my @files; for my $file (@{$args{vcf_in}}) { bgzip_tabix_vcf($opts,$file); push @files, "$$opts{tmp}/$file.vcf.gz"; } my $files = join(' ',@files); bgzip_tabix($opts,file=>$args{tab_in},suffix=>'tab',args=>'-s 1 -b 2 -e 3'); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools isec $args{args} -T $$opts{tmp}/$args{tab_in}.tab.gz $files 2>/dev/null | grep -v ^##bcftools_"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools isec -Ob $args{args} -T $$opts{tmp}/$args{tab_in}.tab.gz $files 2>/dev/null | $$opts{bin}/bcftools view | grep -v ^##bcftools_"); } sub test_vcf_query { my ($opts,%args) = @_; bgzip_tabix_vcf($opts,$args{in}); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools query $args{args} $$opts{tmp}/$args{in}.vcf.gz"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools view -Ob $$opts{tmp}/$args{in}.vcf.gz | $$opts{bin}/bcftools query $args{args}"); } sub test_vcf_convert { my ($opts,%args) = @_; bgzip_tabix_vcf($opts,$args{in}); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools convert $args{args} $$opts{tmp}/$args{in}.vcf.gz"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools view -Ob $$opts{tmp}/$args{in}.vcf.gz | $$opts{bin}/bcftools convert $args{args}"); } sub test_vcf_convert_gvcf { my ($opts,%args) = @_; bgzip_tabix_vcf($opts,$args{in}); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools convert $args{args} $$opts{tmp}/$args{in}.vcf.gz | grep -v ^##bcftools"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools view -Ob $$opts{tmp}/$args{in}.vcf.gz | $$opts{bin}/bcftools convert $args{args} | grep -v ^##bcftools"); } sub test_vcf_convert_tsv2vcf { my ($opts,%args) = @_; my $params = ''; if ( exists($args{args}) ) { $params .= " $args{args}"; } if ( exists($args{fai} ) ) { $params .= " -f $$opts{path}/$args{fai}.fa"; } test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools convert $params --tsv2vcf $$opts{path}/$args{in} | grep -v ^##bcftools_"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools convert -Ou $params --tsv2vcf $$opts{path}/$args{in} | $$opts{bin}/bcftools view | grep -v ^##bcftools_"); } sub test_vcf_norm { my ($opts,%args) = @_; bgzip_tabix_vcf($opts,$args{in}); my $params = ''; if ( exists($args{args}) ) { $params .= " $args{args}"; } if ( exists($args{fai} ) ) { $params .= " -f $$opts{path}/$args{fai}.fa"; } test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools norm $params $$opts{tmp}/$args{in}.vcf.gz | grep -v ^##bcftools_"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools norm -Ob $params $$opts{tmp}/$args{in}.vcf.gz | $$opts{bin}/bcftools view | grep -v ^##bcftools_"); } sub test_vcf_view { my ($opts,%args) = @_; bgzip_tabix_vcf($opts,$args{in}); if ( !exists($args{args}) ) { $args{args} = ''; } if ( exists($args{tgts}) ) { $args{args} .= "-T $$opts{path}/$args{tgts}"; } test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools view $args{args} $$opts{tmp}/$args{in}.vcf.gz $args{reg} | grep -v ^##bcftools_"); unless ($args{args} =~ /-H/) { test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools view -Ob $args{args} $$opts{tmp}/$args{in}.vcf.gz $args{reg} | $$opts{bin}/bcftools view | grep -v ^##bcftools_"); } } sub test_vcf_call { my ($opts,%args) = @_; test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools call $args{args} $$opts{path}/$args{in}.vcf | grep -v ^##bcftools_"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools call -Ob $args{args} $$opts{path}/$args{in}.vcf | $$opts{bin}/bcftools view | grep -v ^##bcftools_"); } sub test_vcf_call_cAls { my ($opts,%args) = @_; bgzip_tabix($opts,file=>$args{tab},suffix=>'tab',args=>'-s1 -b2 -e2'); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools call -mA -C alleles -T $$opts{tmp}/$args{tab}.tab.gz $$opts{path}/$args{in}.vcf | grep -v ^##bcftools_"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools call -Ob -mA -C alleles -T $$opts{tmp}/$args{tab}.tab.gz $$opts{path}/$args{in}.vcf | $$opts{bin}/bcftools view | grep -v ^##bcftools_"); } sub test_vcf_filter { my ($opts,%args) = @_; my $pipe = 'grep -v ^##bcftools_'; if ( exists($args{fmt}) ) { $pipe = "$$opts{bin}/bcftools query -f '$args{fmt}'"; } test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools filter $args{args} $$opts{path}/$args{in}.vcf | $pipe"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools filter -Ob $args{args} $$opts{path}/$args{in}.vcf | $$opts{bin}/bcftools view | $pipe"); } sub test_vcf_regions { my ($opts,%args) = @_; bgzip_tabix_vcf($opts,$args{in}); # regions vs targets, holding tab in memory my $query = q[%CHROM %POS %REF,%ALT\n]; test_cmd($opts,cmd=>qq[$$opts{bin}/bcftools query -f'$query' -T $$opts{path}/$args{in}.tab $$opts{tmp}/$args{in}.vcf.gz],out=>'regions.out'); test_cmd($opts,cmd=>qq[$$opts{bin}/bcftools view -Ob $$opts{tmp}/$args{in}.vcf.gz | $$opts{bin}/bcftools query -f'$query' -T $$opts{path}/$args{in}.tab],out=>'regions.out'); test_cmd($opts,cmd=>qq[$$opts{bin}/bcftools query -f'$query' -R $$opts{path}/$args{in}.tab $$opts{tmp}/$args{in}.vcf.gz],out=>'regions.out'); # regions vs targets, reading tabix-ed tab cmd(qq[cat $$opts{path}/$args{in}.tab | $$opts{bgzip} -c > $$opts{tmp}/$args{in}.tab.gz]); cmd(qq[$$opts{tabix} -f -s1 -b2 -e3 $$opts{tmp}/$args{in}.tab.gz]); test_cmd($opts,cmd=>qq[$$opts{bin}/bcftools query -f'$query' -T $$opts{tmp}/$args{in}.tab.gz $$opts{tmp}/$args{in}.vcf.gz],out=>'regions.out'); test_cmd($opts,cmd=>qq[$$opts{bin}/bcftools view -Ob $$opts{tmp}/$args{in}.vcf.gz | $$opts{bin}/bcftools query -f'$query' -T $$opts{tmp}/$args{in}.tab.gz],out=>'regions.out'); test_cmd($opts,cmd=>qq[$$opts{bin}/bcftools query -f'$query' -R $$opts{tmp}/$args{in}.tab.gz $$opts{tmp}/$args{in}.vcf.gz],out=>'regions.out'); # regions vs targets, holding bed in memory cmd(qq[cat $$opts{path}/$args{in}.tab | awk '{OFS="\\t"}{print \$1,\$2-1,\$3}' > $$opts{tmp}/$args{in}.bed]); test_cmd($opts,cmd=>qq[$$opts{bin}/bcftools query -f'$query' -T $$opts{tmp}/$args{in}.bed $$opts{tmp}/$args{in}.vcf.gz],out=>'regions.out'); test_cmd($opts,cmd=>qq[$$opts{bin}/bcftools view -Ob $$opts{tmp}/$args{in}.vcf.gz | $$opts{bin}/bcftools query -f'$query' -T $$opts{tmp}/$args{in}.bed],out=>'regions.out'); test_cmd($opts,cmd=>qq[$$opts{bin}/bcftools query -f'$query' -R $$opts{tmp}/$args{in}.bed $$opts{tmp}/$args{in}.vcf.gz],out=>'regions.out'); # regions vs targets, reading tabix-ed bed cmd(qq[cat $$opts{tmp}/$args{in}.bed | $$opts{bgzip} -c > $$opts{tmp}/$args{in}.bed.gz]); cmd(qq[$$opts{tabix} -f -p bed $$opts{tmp}/$args{in}.bed.gz]); test_cmd($opts,cmd=>qq[$$opts{bin}/bcftools query -f'$query' -T $$opts{tmp}/$args{in}.bed.gz $$opts{tmp}/$args{in}.vcf.gz],out=>'regions.out'); test_cmd($opts,cmd=>qq[$$opts{bin}/bcftools view -Ob $$opts{tmp}/$args{in}.vcf.gz | $$opts{bin}/bcftools query -f'$query' -T $$opts{tmp}/$args{in}.bed.gz],out=>'regions.out'); test_cmd($opts,cmd=>qq[$$opts{bin}/bcftools query -f'$query' -R $$opts{tmp}/$args{in}.bed.gz $$opts{tmp}/$args{in}.vcf.gz],out=>'regions.out'); } sub test_usage { my ($opts,%args) = @_; my $test = "test_usage"; print "$test:\n"; print "\t$args{cmd}\n"; my $tty_input; if (-t) { $args{redirection} = ""; # no redirection necessary } elsif (eval { require IO::Pty }) { $tty_input = new IO::Pty; # ensure stdin is a terminal, so that subcommands display their usage $args{redirection} = "<'" . $tty_input->ttyname . "'"; } else { warn "$0: module IO::Pty not found; skipping usage tests\n"; return; } my $command = $args{cmd}; my $commandpath = $$opts{bin}."/".$command; my ($ret,$out) = _cmd("$commandpath $args{redirection} 2>&1"); if ( $out =~ m/\/bin\/bash.*no.*such/i ) { failed($opts,$test,"could not run $commandpath: $out"); return; } my @sections = ($out =~ m/(^[A-Za-z]+.*?)(?:(?=^[A-Za-z]+:)|\z)/msg); my $have_usage = 0; my $have_version = 0; my $have_subcommands = 0; my $usage = ""; my @subcommands = (); foreach my $section (@sections) { if ( $section =~ m/^usage/i ) { $have_usage = 1; $section =~ s/^[[:word:]]+[[:punct:]]?[[:space:]]*//; $usage = $section; } elsif ( $section =~ m/^version/i ) { $have_version = 1; } elsif ( $section =~ m/^command/i ) { $have_subcommands = 1; foreach my $line (split /\n/, $section) { push @subcommands, $1 if $line =~ /^\s{2,}(\w+)\s{2,}/; } } } if ( !$have_usage ) { failed($opts,$test,"did not have Usage:"); return; } if ( !$have_version ) { failed($opts,$test,"did not have Version:"); return; } if ( !$have_subcommands ) { failed($opts,$test,"did not have Commands:"); return; } if ( !($usage =~ m/$command/) ) { failed($opts,$test,"usage did not mention $command"); return; } if ( scalar(@subcommands) < 1 ) { failed($opts,$test,"could not parse subcommands"); return; } passed($opts,$test); # now test subcommand usage as well foreach my $subcommand (@subcommands) { test_usage_subcommand($opts,%args,subcmd=>$subcommand); } } sub test_usage_subcommand { my ($opts,%args) = @_; my $test = "test_usage_subcommand"; print "$test:\n"; print "\t$args{cmd} $args{subcmd}\n"; my $command = $args{cmd}; my $subcommand = $args{subcmd}; my $commandpath = $$opts{bin}."/".$command; my ($ret,$out) = _cmd("$commandpath $subcommand $args{redirection} 2>&1"); if ( $out =~ m/\/bin\/bash.*no.*such/i ) { failed($opts,$test,"could not run $commandpath $subcommand: $out"); return; } my @sections = ($out =~ m/(^[A-Za-z]+.*?)(?:(?=^[A-Za-z]+:)|\z)/msg); my $have_usage = 0; my $usage = ""; foreach my $section (@sections) { if ( $section =~ m/^usage/i ) { $have_usage = 1; $section =~ s/^[[:word:]]+[[:punct:]]?[[:space:]]*//; $usage = $section; } } if ( !$have_usage ) { failed($opts,$test,"did not have Usage:"); return; } if ( !($usage =~ m/$command[[:space:]]+$subcommand/) ) { failed($opts,$test,"usage did not mention $command $subcommand"); return; } passed($opts,$test); } sub test_vcf_annotate { my ($opts,%args) = @_; my ($annot_fname,$in_fname,$hdr); if ( exists($args{tab}) ) { bgzip_tabix($opts,file=>$args{tab},suffix=>'tab',args=>'-s1 -b2 -e2'); $annot_fname = "-a $$opts{tmp}/$args{tab}.tab.gz"; $in_fname = "$$opts{path}/$args{in}.vcf"; $hdr = "-h $$opts{path}/$args{in}.hdr"; } elsif ( exists($args{vcf}) ) { bgzip_tabix_vcf($opts,"$args{in}"); bgzip_tabix_vcf($opts,$args{vcf}); $annot_fname = "-a $$opts{tmp}/$args{vcf}.vcf.gz"; $in_fname = "$$opts{tmp}/$args{in}.vcf.gz"; $hdr = ''; } else { $in_fname = "$$opts{path}/$args{in}.vcf"; $annot_fname = ''; $hdr = ''; } test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools annotate $annot_fname $hdr $args{args} $in_fname | $$opts{bin}/bcftools view | grep -v ^##bcftools_"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools annotate -Ob $annot_fname $hdr $args{args} $in_fname | $$opts{bin}/bcftools view | grep -v ^##bcftools_"); } sub test_vcf_plugin { my ($opts,%args) = @_; if ( !$$opts{test_plugins} ) { return; } $ENV{BCFTOOLS_PLUGINS} = "$$opts{bin}/plugins"; if ( !exists($args{args}) ) { $args{args} = ''; } $args{args} =~ s/{PATH}/$$opts{path}/g; bgzip_tabix_vcf($opts,"$args{in}"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools $args{cmd} $$opts{tmp}/$args{in}.vcf.gz $args{args} | grep -v ^##bcftools_"); cmd("$$opts{bin}/bcftools view -Ob $$opts{tmp}/$args{in}.vcf.gz > $$opts{tmp}/$args{in}.bcf"); cmd("$$opts{bin}/bcftools index -f $$opts{tmp}/$args{in}.bcf"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools $args{cmd} $$opts{tmp}/$args{in}.bcf $args{args} | grep -v ^##bcftools_"); } sub test_vcf_concat { my ($opts,%args) = @_; my $files; for my $file (@{$args{in}}) { if ( $args{do_bcf} ) { cmd("$$opts{bin}/bcftools view -Ob $$opts{tmp}/$file.vcf.gz > $$opts{tmp}/$file.bcf"); cmd("$$opts{bin}/bcftools index -f $$opts{tmp}/$file.bcf"); $files .= " $$opts{tmp}/$file.bcf"; } else { bgzip_tabix_vcf($opts,$file); $files .= " $$opts{tmp}/$file.vcf.gz"; } } test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools concat $args{args} $files | grep -v ^##bcftools_"); test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools concat -Ob $args{args} $files | $$opts{bin}/bcftools view | grep -v ^##bcftools_"); } sub test_vcf_reheader { my ($opts,%args) = @_; cmd("$$opts{bin}/bcftools view -Ob $$opts{path}/$args{in}.vcf > $$opts{tmp}/$args{in}.bcf"); cmd("$$opts{bin}/bcftools view -Oz $$opts{path}/$args{in}.vcf > $$opts{tmp}/$args{in}.vcf.gz"); my $arg = exists($args{header}) ? "-h $$opts{path}/$args{header}" : "-s $$opts{path}/$args{samples}"; for my $file ("$$opts{path}/$args{in}.vcf","$$opts{tmp}/$args{in}.bcf","$$opts{tmp}/$args{in}.vcf.gz") { # bcf header lines can come in different order my %bcf_args = (); if ( $file=~/\.bcf$/ && -e "$$opts{path}/$args{out}.bcf" ) { %bcf_args = ( out=>"$args{out}.bcf" ); } test_cmd($opts,%args,%bcf_args,cmd=>"$$opts{bin}/bcftools reheader $arg $file | $$opts{bin}/bcftools view | grep -v ^##bcftools_"); test_cmd($opts,%args,%bcf_args,cmd=>"cat $file | $$opts{bin}/bcftools reheader $arg | $$opts{bin}/bcftools view | grep -v ^##bcftools_"); } } sub test_rename_chrs { my ($opts,%args) = @_; cmd("$$opts{bin}/bcftools view -Ob $$opts{path}/$args{in}.vcf > $$opts{tmp}/$args{in}.bcf"); cmd("$$opts{bin}/bcftools query -f'chr%CHROM\\t%POS\\n' $$opts{path}/$args{in}.vcf > $$opts{path}/rename.out.tmp"); cmd("$$opts{bin}/bcftools query -f'%CHROM\\tchr%CHROM\\n' $$opts{path}/$args{in}.vcf | uniq > $$opts{tmp}/rename.map"); my $prevfailed = $$opts{nfailed}; for my $file ("$$opts{tmp}/$args{in}.bcf","$$opts{path}/$args{in}.vcf") { test_cmd($opts,%args,out=>"rename.out.tmp",cmd=>"$$opts{bin}/bcftools annotate --rename-chrs $$opts{tmp}/rename.map -Ov $file | $$opts{bin}/bcftools query -f'%CHROM\\t%POS\\n'"); test_cmd($opts,%args,out=>"rename.out.tmp",cmd=>"$$opts{bin}/bcftools annotate --rename-chrs $$opts{tmp}/rename.map -Ob $file | $$opts{bin}/bcftools query -f'%CHROM\\t%POS\\n'"); } unlink "$$opts{path}/rename.out.tmp" if $$opts{nfailed} == $prevfailed; } sub test_vcf_consensus { my ($opts,%args) = @_; bgzip_tabix_vcf($opts,$args{in}); my $mask = $args{mask} ? "-m $$opts{path}/$args{mask}" : ''; my $chain = $args{chain} ? "-c $$opts{tmp}/$args{chain}" : ''; test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools consensus $$opts{tmp}/$args{in}.vcf.gz -f $$opts{path}/$args{fa} $args{args} $mask $chain"); } sub test_vcf_consensus_chain { my ($opts,%args) = @_; bgzip_tabix_vcf($opts,$args{in}); my $mask = $args{mask} ? "-m $$opts{path}/$args{mask}" : ''; my $chain = $args{chain} ? "-c $$opts{tmp}/$args{chain}.new" : ''; test_cmd($opts,%args,cmd=>"$$opts{bin}/bcftools consensus $$opts{tmp}/$args{in}.vcf.gz -f $$opts{path}/$args{fa} $args{args} $mask $chain > /dev/null; cat $$opts{tmp}/$args{chain}.new"); } bcftools-1.2/test/vcf2sex.out000066400000000000000000000000201246371514100162530ustar00rootroot00000000000000MALE M FEMALE F bcftools-1.2/test/vcf2sex.vcf000066400000000000000000000015331246371514100162340ustar00rootroot00000000000000##fileformat=VCFv4.2 ##FORMAT= ##contig= ##contig= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT MALE FEMALE X 1 . C A . PASS . GT 1 0/1 X 12000 . C A . PASS . GT 0 0/1 X 24000 . C A . PASS . GT 1 0/1 X 36000 . C A . PASS . GT 0 0/1 X 48000 . C A . PASS . GT 1 0/1 X 60000 . C A . PASS . GT 0 0/1 X 100000 . C A . PASS . GT 0/1 0/1 X 2699521 . C A . PASS . GT 0 0/1 X 33145825 . C A . PASS . GT 1 0/1 X 63592129 . C A . PASS . GT 1 0/1 X 94038433 . C A . PASS . GT 0 0/1 X 124484737 . C A . PASS . GT 0 0/1 X 154931043 . C A . PASS . GT 0/1 0/1 Y 1 . C A . PASS . GT 0 . Y 11874713 . C A . PASS . GT 0 . Y 23749426 . C A . PASS . GT 0 . Y 35624139 . C A . PASS . GT 0 . Y 47498852 . C A . PASS . GT 0 . Y 59373565 . C A . PASS . GT 0 . bcftools-1.2/test/view.1.out000066400000000000000000000072671246371514100160300ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##reference=file:///seq/references/1000Genomes-NCBI37.fasta ##contig= ##contig= ##contig= ##contig= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT NA00002 20 138125 rs2298108 G T 999 PASS DP4=174391,20849,82080,4950;DP=286107;Dels=0;FS=3200;HWE=0.199462;ICF=0.01858;MQ0=0;MQ=46;PV4=0,0,0,1;QD=17.22;AN=2;AC=1 GT:PL:DP:GQ 0/1:140,0,255:71:99 20 138148 rs2298109 C T 999 PASS DP4=194136,45753,94945,14367;DP=356657;Dels=0;FS=3200;HWE=0.177865;ICF=0.0198;MQ0=0;MQ=47;PV4=0,0,0,1;QD=14.57;AN=2;AC=1 GT:PL:DP:GQ 0/1:192,0,255:82:99 20 304568 . C T 999 PASS DP4=16413,4543,945,156;DP=43557;Dels=0;FS=3200;HWE=0.076855;ICF=0.0213;MQ0=0;MQ=50;PV4=0,0,0,1;QD=15.45;AN=2;AC=1 GT:PL:DP:GQ 0|1:192,0,255:13:99 X 2942109 rs5939407 T C 999 PASS DP4=23273,27816,40128,48208;DP=146673;Dels=0;FS=43.639;HWE=0.622715;ICF=-0.01176;MQ0=1;MQ=46;PV4=0.65,1,0,1;QD=14.81;AN=1;AC=1 GT:PL:DP:GQ 1:255,0:33:99 X 3048719 . T C 999 PASS DP4=13263,27466,40128,48208;DP=146673;Dels=0;FS=43.639;HWE=0.622715;ICF=-0.01176;MQ0=1;MQ=46;PV4=0.65,1,0,1;QD=14.81;AN=1;AC=1 GT:PL:DP:GQ 1:255,0:33:99 Y 8657215 . C A 999 PASS DP4=74915,114274,1948,2955;DP=195469;Dels=0;FS=3.181;MQ0=0;MQ=50;PV4=0.86,1,0,1;QD=33.77;AN=1;AC=1 GT:PL:DP:GQ 1:255,0:64:99 Y 10011673 rs78249411 G A 999 MinAB DP4=47351,30839,178796,279653;DP=550762;Dels=0;FS=41.028;MQ0=37362;MQ=26;PV4=0,0,0,1;QD=17.45;AN=1;AC=1 GT:PL:DP:GQ 1:95,0:130:99 bcftools-1.2/test/view.10.out000066400000000000000000000061041246371514100160750ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##reference=file:///seq/references/1000Genomes-NCBI37.fasta ##contig= ##contig= ##contig= ##contig= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT NA00001 NA00002 NA00003 11 2343543 . A . 999 PASS DP=100223 GT:PL:DP:GQ 0/0:0,255,255:193:99 0/0:0,255,255:211:99 0/0:0,255,255:182:99 11 5464562 . C T 999 PASS DP=0 GT:PL:DP:GQ ./.:0,0,0:.:. ./.:0,0,0:.:. ./.:0,0,0:.:. X 3048719 . T C 999 PASS DP4=13263,27466,40128,48208;DP=146673;Dels=0;FS=43.639;HWE=0.622715;ICF=-0.01176;MQ0=1;MQ=46;PV4=0.65,1,0,1;QD=14.81;AN=4;AC=3 GT:PL:DP:GQ 0:0,255:20:99 1:255,0:33:99 0|1:255,0,157:52:99 Y 8657215 . C A 999 PASS DP4=74915,114274,1948,2955;DP=195469;Dels=0;FS=3.181;MQ0=0;MQ=50;PV4=0.86,1,0,1;QD=33.77;AN=2;AC=1 GT:PL:DP:GQ 0:0,255:47:99 1:255,0:64:99 .:.:.:. bcftools-1.2/test/view.2.out000066400000000000000000000060521246371514100160200ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##reference=file:///seq/references/1000Genomes-NCBI37.fasta ##contig= ##contig= ##contig= ##contig= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT NA00003 20 76962 rs6111385 T C 999 PASS DP4=110138,70822,421911,262673;DP=911531;Dels=0;FS=21.447;HWE=0.491006;ICF=-0.01062;MQ0=1;MQ=46;PV4=2.5e-09,0,0,1;QD=22.31;AC=2;AN=2 GT:PL:DP:GQ 1/1:255,255,0:182:99 20 138125 rs2298108 G T 999 PASS DP4=174391,20849,82080,4950;DP=286107;Dels=0;FS=3200;HWE=0.199462;ICF=0.01858;MQ0=0;MQ=46;PV4=0,0,0,1;QD=17.22;AN=2;AC=2 GT:PL:DP:GQ 1/1:255,199,0:66:99 20 138148 rs2298109 C T 999 PASS DP4=194136,45753,94945,14367;DP=356657;Dels=0;FS=3200;HWE=0.177865;ICF=0.0198;MQ0=0;MQ=47;PV4=0,0,0,1;QD=14.57;AN=2;AC=2 GT:PL:DP:GQ 1/1:255,235,0:78:99 bcftools-1.2/test/view.3.out000066400000000000000000000055211246371514100160210ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##reference=file:///seq/references/1000Genomes-NCBI37.fasta ##contig= ##contig= ##contig= ##contig= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT NA00003 X 2928329 rs62584840 C T 999 PASS DP4=302,9137,32,1329;DP=11020;Dels=0;FS=13.38;HWE=0.284332;ICF=0.0253;MQ0=0;MQ=49;PV4=0.094,0,0,1;QD=18.61;AN=2;AC=1 GT:PL:DP:GQ 0/1:73,0,19:4:30 X 2933066 rs61746890 G C 999 PASS DP4=69865,100561,461,783;DP=173729;Dels=0;FS=10.833;MQ0=0;MQ=50;PV4=0.005,3.6e-14,0,1;QD=15.33;AN=2;AC=1 GT:PL:DP:GQ 0/1:255,255,255:62:99 bcftools-1.2/test/view.4.out000066400000000000000000000056601246371514100160260ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##reference=file:///seq/references/1000Genomes-NCBI37.fasta ##contig= ##contig= ##contig= ##contig= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT NA00001 NA00002 NA00003 X 2942109 rs5939407 T C 999 PASS DP4=23273,27816,40128,48208;DP=146673;Dels=0;FS=43.639;HWE=0.622715;ICF=-0.01176;MQ0=1;MQ=46;PV4=0.65,1,0,1;QD=14.81;AN=4;AC=3 GT:PL:DP:GQ 0:0,255:20:99 1:255,0:33:99 1/1:255,157,0:52:99 X 3048719 . T C 999 PASS DP4=13263,27466,40128,48208;DP=146673;Dels=0;FS=43.639;HWE=0.622715;ICF=-0.01176;MQ0=1;MQ=46;PV4=0.65,1,0,1;QD=14.81;AN=4;AC=3 GT:PL:DP:GQ 0:0,255:20:99 1:255,0:33:99 0|1:255,0,157:52:99 bcftools-1.2/test/view.5.out000066400000000000000000000064201246371514100160220ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##reference=file:///seq/references/1000Genomes-NCBI37.fasta ##contig= ##contig= ##contig= ##contig= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT NA00001 NA00002 NA00003 20 304568 . C T 999 PASS DP4=16413,4543,945,156;DP=43557;Dels=0;FS=3200;HWE=0.076855;ICF=0.0213;MQ0=0;MQ=50;PV4=0,0,0,1;QD=15.45;AN=6;AC=4 GT:PL:DP:GQ 0|1:95,0,255:90:99 0|1:192,0,255:13:99 1|1:255,95,0:60:99 X 3048719 . T C 999 PASS DP4=13263,27466,40128,48208;DP=146673;Dels=0;FS=43.639;HWE=0.622715;ICF=-0.01176;MQ0=1;MQ=46;PV4=0.65,1,0,1;QD=14.81;AN=4;AC=3 GT:PL:DP:GQ 0:0,255:20:99 1:255,0:33:99 0|1:255,0,157:52:99 Y 8657215 . C A 999 PASS DP4=74915,114274,1948,2955;DP=195469;Dels=0;FS=3.181;MQ0=0;MQ=50;PV4=0.86,1,0,1;QD=33.77;AN=2;AC=1 GT:PL:DP:GQ 0:0,255:47:99 1:255,0:64:99 .:.:.:. Y 10011673 rs78249411 G A 999 MinAB DP4=47351,30839,178796,279653;DP=550762;Dels=0;FS=41.028;MQ0=37362;MQ=26;PV4=0,0,0,1;QD=17.45;AN=2;AC=2 GT:PL:DP:GQ 1:126,101:146:37 1:95,0:130:99 .:.:.:. bcftools-1.2/test/view.6.out000066400000000000000000000112621246371514100160230ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##reference=file:///seq/references/1000Genomes-NCBI37.fasta ##contig= ##contig= ##contig= ##contig= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT NA00001 NA00002 NA00003 11 2343543 . A . 999 PASS DP=100223 GT:PL:DP:GQ 0/0:0,255,255:193:99 0/0:0,255,255:211:99 0/0:0,255,255:182:99 11 5464562 . C T 999 PASS DP=0 GT:PL:DP:GQ ./.:0,0,0:.:. ./.:0,0,0:.:. ./.:0,0,0:.:. 20 76962 rs6111385 T C 999 PASS DP4=110138,70822,421911,262673;DP=911531;Dels=0;FS=21.447;HWE=0.491006;ICF=-0.01062;MQ0=1;MQ=46;PV4=2.5e-09,0,0,1;QD=22.31 GT:PL:DP:GQ 0/1:255,0,255:193:99 1/1:255,255,0:211:99 1/1:255,255,0:182:99 20 126310 . ACC A 999 StrandBias;EndDistBias DP4=125718,95950,113812,80890;DP=461867;HWE=0.24036;ICF=0.01738;INDEL;IS=374,0.937343;MQ=49;PV4=9e-30,1,0,3.8e-13;QD=0.0172;AN=6;AC=4 GT:DP:GQ:PL 0/1:117:99:255,0,132 0/1:111:99:255,0,139 1/1:78:99:255,213,0 20 138125 rs2298108 G T 999 PASS DP4=174391,20849,82080,4950;DP=286107;Dels=0;FS=3200;HWE=0.199462;ICF=0.01858;MQ0=0;MQ=46;PV4=0,0,0,1;QD=17.22;AN=6;AC=4 GT:PL:DP:GQ 0/1:135,0,163:66:99 0/1:140,0,255:71:99 1/1:255,199,0:66:99 20 138148 rs2298109 C T 999 PASS DP4=194136,45753,94945,14367;DP=356657;Dels=0;FS=3200;HWE=0.177865;ICF=0.0198;MQ0=0;MQ=47;PV4=0,0,0,1;QD=14.57;AN=6;AC=4 GT:PL:DP:GQ 0/1:195,0,255:87:99 0/1:192,0,255:82:99 1/1:255,235,0:78:99 20 271225 . T TTTA,TA 999 StrandBias DP4=29281,42401,27887,29245;DP=272732;INDEL;IS=95,0.748031;MQ=47;PV4=0,1,0,1;QD=0.0948;AN=6;AC=2,2 GT:DP:GQ:PL 0/2:33:49:151,53,203,0,52,159 0/1:51:99:255,0,213,255,255,255 1/2:47:99:255,255,255,255,0,241 20 326891 . A AC 999 PASS DP4=125718,95950,113812,80890;DP=461867;HWE=0.24036;ICF=0.01738;INDEL;IS=374,0.937343;MQ=49;PV4=9e-30,1,0,3.8e-13;QD=0.0172;AN=4;AC=2 GT:DP:GQ:PL 0|1:117:99:255,0,132 0|1:111:99:255,0,139 ./.:.:.:.,.,. X 2928329 rs62584840 C T 999 PASS DP4=302,9137,32,1329;DP=11020;Dels=0;FS=13.38;HWE=0.284332;ICF=0.0253;MQ0=0;MQ=49;PV4=0.094,0,0,1;QD=18.61;AN=4;AC=1 GT:PL:DP:GQ 0:0,56:2:73 0:0,81:3:98 0/1:73,0,19:4:30 X 2933066 rs61746890 G C 999 PASS DP4=69865,100561,461,783;DP=173729;Dels=0;FS=10.833;MQ0=0;MQ=50;PV4=0.005,3.6e-14,0,1;QD=15.33;AN=4;AC=1 GT:PL:DP:GQ 0:0,255:39:99 0:0,255:37:99 0/1:255,255,255:62:99 X 2942109 rs5939407 T C 999 PASS DP4=23273,27816,40128,48208;DP=146673;Dels=0;FS=43.639;HWE=0.622715;ICF=-0.01176;MQ0=1;MQ=46;PV4=0.65,1,0,1;QD=14.81;AN=4;AC=3 GT:PL:DP:GQ 0:0,255:20:99 1:255,0:33:99 1/1:255,157,0:52:99 bcftools-1.2/test/view.7.out000066400000000000000000000050001246371514100160150ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##reference=file:///seq/references/1000Genomes-NCBI37.fasta ##contig= ##contig= ##contig= ##contig= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT NA00001 NA00002 NA00003 bcftools-1.2/test/view.8.out000066400000000000000000000001371246371514100160240ustar00rootroot0000000000000011 5464562 . C T 999 PASS DP=0;AC=0;AN=0 GT:PL:DP:GQ ./.:0,0,0:.:. ./.:0,0,0:.:. ./.:0,0,0:.:. bcftools-1.2/test/view.9.out000066400000000000000000000057401246371514100160320ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##reference=file:///seq/references/1000Genomes-NCBI37.fasta ##contig= ##contig= ##contig= ##contig= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO 11 2343543 . A . 999 PASS DP=100223 20 126310 . ACC A 999 StrandBias;EndDistBias DP4=125718,95950,113812,80890;DP=461867;HWE=0.24036;ICF=0.01738;INDEL;IS=374,0.937343;MQ=49;PV4=9e-30,1,0,3.8e-13;QD=0.0172;AN=6;AC=4 20 271225 . T TTTA,TA 999 StrandBias DP4=29281,42401,27887,29245;DP=272732;INDEL;IS=95,0.748031;MQ=47;PV4=0,1,0,1;QD=0.0948;AN=6;AC=2,2 20 326891 . A AC 999 PASS DP4=125718,95950,113812,80890;DP=461867;HWE=0.24036;ICF=0.01738;INDEL;IS=374,0.937343;MQ=49;PV4=9e-30,1,0,3.8e-13;QD=0.0172;AN=4;AC=2 bcftools-1.2/test/view.chrs.out000066400000000000000000000055261246371514100166230ustar00rootroot00000000000000##fileformat=VCFv4.1 ##FILTER= ##contig= ##contig= ##contig= ##FORMAT= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT AAA BBB Pf3D7_01_v3 1 . A T . . . GT . . Pf3D7_01_v3 2 . A G . . . GT . . Pf3D7_01_v3 33 . T A . . . GT . . Pf3D7_01_v3 36 . A G . . . GT . . Pf3D7_01_v3 57 . C T . . . GT . . Pf3D7_01_v3 61 . A T . . . GT . . Pf3D7_01_v3 72 . T C,A . . . GT . . Pf3D7_01_v3 73 . T A . . . GT . . Pf3D7_01_v3 74 . A T,C . . . GT . . Pf3D7_01_v3 84 . C T . . . GT . . Pf3D7_01_v3 110 . T A . . . GT . . Pf3D7_01_v3 111 . T A . . . GT . . Pf3D7_01_v3 129 . T A . . . GT . . Pf3D7_01_v3 146 . A C,T . . . GT . . Pf3D7_01_v3 147 . A C,T . . . GT . . Pf3D7_01_v3 152 . G T . . . GT . . Pf3D7_01_v3 168 . T A . . . GT . . Pf3D7_01_v3 169 . A G . . . GT . . Pf3D7_01_v3 175 . A T . . . GT . . Pf3D7_01_v3 195 . T A . . . GT . . Pf3D7_01_v3 196 . A C . . . GT . . Pf3D7_01_v3 199 . T C . . . GT . . Pf3D7_01_v3 204 . C T . . . GT . . Pf3D7_01_v3 213 . G A . . . GT . . Pf3D7_01_v3 222 . A G . . . GT . . Pf3D7_01_v3 223 . A G . . . GT . . Pf3D7_01_v3 227 . T A . . . GT . . Pf3D7_02_v3 90 . C T . . . GT . . Pf3D7_02_v3 100 . T A . . . GT . . Pf3D7_02_v3 111 . T A . . . GT . . Pf3D7_02_v3 129 . T A . . . GT . . Pf3D7_02_v3 146 . A C,T . . . GT . . Pf3D7_02_v3 147 . A C,T . . . GT . . Pf3D7_02_v3 152 . G T . . . GT . . Pf3D7_02_v3 168 . T A . . . GT . . Pf3D7_02_v3 169 . A G . . . GT . . Pf3D7_02_v3 175 . A T . . . GT . . Pf3D7_02_v3 195 . T A . . . GT . . Pf3D7_02_v3 196 . A C . . . GT . . Pf3D7_02_v3 199 . T C . . . GT . . Pf3D7_02_v3 204 . C T . . . GT . . Pf3D7_02_v3 213 . G A . . . GT . . Pf3D7_02_v3 222 . A G . . . GT . . Pf3D7_02_v3 223 . A G . . . GT . . Pf3D7_02_v3 227 . T A . . . GT . . Pf3D7_02_v3 235 . A G . . . GT . . Pf3D7_03_v3 1 . A T . . . GT . . Pf3D7_03_v3 2 . A G . . . GT . . Pf3D7_03_v3 33 . T A . . . GT . . Pf3D7_03_v3 36 . A G . . . GT . . Pf3D7_03_v3 57 . C T . . . GT . . Pf3D7_03_v3 61 . A T . . . GT . . Pf3D7_03_v3 72 . T C,A . . . GT . . Pf3D7_03_v3 73 . T A . . . GT . . Pf3D7_03_v3 74 . A T,C . . . GT . . Pf3D7_03_v3 84 . C T . . . GT . . Pf3D7_03_v3 110 . T A . . . GT . . Pf3D7_03_v3 111 . T A . . . GT . . Pf3D7_03_v3 129 . T A . . . GT . . Pf3D7_03_v3 146 . A C,T . . . GT . . Pf3D7_03_v3 147 . A C,T . . . GT . . Pf3D7_03_v3 152 . G T . . . GT . . Pf3D7_03_v3 168 . T A . . . GT . . Pf3D7_03_v3 169 . A G . . . GT . . Pf3D7_03_v3 175 . A T . . . GT . . Pf3D7_03_v3 195 . T A . . . GT . . Pf3D7_03_v3 196 . A C . . . GT . . Pf3D7_03_v3 199 . T C . . . GT . . Pf3D7_03_v3 204 . C T . . . GT . . Pf3D7_03_v3 213 . G A . . . GT . . Pf3D7_03_v3 222 . A G . . . GT . . Pf3D7_03_v3 223 . A G . . . GT . . Pf3D7_03_v3 227 . T A . . . GT . . Pf3D7_03_v3 235 . A G . . . GT . . bcftools-1.2/test/view.chrs.tab000066400000000000000000000022071246371514100165530ustar00rootroot00000000000000Pf3D7_01_v3 1 Pf3D7_01_v3 2 Pf3D7_01_v3 33 Pf3D7_01_v3 36 Pf3D7_01_v3 57 Pf3D7_01_v3 61 Pf3D7_01_v3 72 Pf3D7_01_v3 73 Pf3D7_01_v3 74 Pf3D7_01_v3 84 Pf3D7_01_v3 110 Pf3D7_01_v3 111 Pf3D7_01_v3 129 Pf3D7_01_v3 146 Pf3D7_01_v3 147 Pf3D7_01_v3 152 Pf3D7_01_v3 168 Pf3D7_01_v3 169 Pf3D7_01_v3 175 Pf3D7_01_v3 195 Pf3D7_01_v3 196 Pf3D7_01_v3 199 Pf3D7_01_v3 204 Pf3D7_01_v3 213 Pf3D7_01_v3 222 Pf3D7_01_v3 223 Pf3D7_01_v3 227 Pf3D7_02_v3 90 Pf3D7_02_v3 100 Pf3D7_02_v3 111 Pf3D7_02_v3 129 Pf3D7_02_v3 146 Pf3D7_02_v3 147 Pf3D7_02_v3 152 Pf3D7_02_v3 168 Pf3D7_02_v3 169 Pf3D7_02_v3 175 Pf3D7_02_v3 195 Pf3D7_02_v3 196 Pf3D7_02_v3 199 Pf3D7_02_v3 204 Pf3D7_02_v3 213 Pf3D7_02_v3 222 Pf3D7_02_v3 223 Pf3D7_02_v3 227 Pf3D7_02_v3 235 Pf3D7_03_v3 1 Pf3D7_03_v3 2 Pf3D7_03_v3 33 Pf3D7_03_v3 36 Pf3D7_03_v3 57 Pf3D7_03_v3 61 Pf3D7_03_v3 72 Pf3D7_03_v3 73 Pf3D7_03_v3 74 Pf3D7_03_v3 84 Pf3D7_03_v3 110 Pf3D7_03_v3 111 Pf3D7_03_v3 129 Pf3D7_03_v3 146 Pf3D7_03_v3 147 Pf3D7_03_v3 152 Pf3D7_03_v3 168 Pf3D7_03_v3 169 Pf3D7_03_v3 175 Pf3D7_03_v3 195 Pf3D7_03_v3 196 Pf3D7_03_v3 199 Pf3D7_03_v3 204 Pf3D7_03_v3 213 Pf3D7_03_v3 222 Pf3D7_03_v3 223 Pf3D7_03_v3 227 Pf3D7_03_v3 235 bcftools-1.2/test/view.chrs.vcf000066400000000000000000000061711246371514100165670ustar00rootroot00000000000000##fileformat=VCFv4.1 ##contig= ##contig= ##contig= ##FORMAT= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT AAA BBB Pf3D7_01_v3 1 . A T . . . GT . . Pf3D7_01_v3 2 . A G . . . GT . . Pf3D7_01_v3 33 . T A . . . GT . . Pf3D7_01_v3 36 . A G . . . GT . . Pf3D7_01_v3 57 . C T . . . GT . . Pf3D7_01_v3 61 . A T . . . GT . . Pf3D7_01_v3 72 . T C,A . . . GT . . Pf3D7_01_v3 73 . T A . . . GT . . Pf3D7_01_v3 74 . A T,C . . . GT . . Pf3D7_01_v3 84 . C T . . . GT . . Pf3D7_01_v3 110 . T A . . . GT . . Pf3D7_01_v3 111 . T A . . . GT . . Pf3D7_01_v3 129 . T A . . . GT . . Pf3D7_01_v3 146 . A C,T . . . GT . . Pf3D7_01_v3 147 . A C,T . . . GT . . Pf3D7_01_v3 152 . G T . . . GT . . Pf3D7_01_v3 168 . T A . . . GT . . Pf3D7_01_v3 169 . A G . . . GT . . Pf3D7_01_v3 175 . A T . . . GT . . Pf3D7_01_v3 195 . T A . . . GT . . Pf3D7_01_v3 196 . A C . . . GT . . Pf3D7_01_v3 199 . T C . . . GT . . Pf3D7_01_v3 204 . C T . . . GT . . Pf3D7_01_v3 213 . G A . . . GT . . Pf3D7_01_v3 222 . A G . . . GT . . Pf3D7_01_v3 223 . A G . . . GT . . Pf3D7_01_v3 227 . T A . . . GT . . Pf3D7_01_v3 235 . A G . . . GT . . Pf3D7_02_v3 1 . A T . . . GT . . Pf3D7_02_v3 2 . A G . . . GT . . Pf3D7_02_v3 33 . T A . . . GT . . Pf3D7_02_v3 36 . A G . . . GT . . Pf3D7_02_v3 57 . C T . . . GT . . Pf3D7_02_v3 61 . A T . . . GT . . Pf3D7_02_v3 72 . T C,A . . . GT . . Pf3D7_02_v3 73 . T A . . . GT . . Pf3D7_02_v3 74 . A T,C . . . GT . . Pf3D7_02_v3 90 . C T . . . GT . . Pf3D7_02_v3 100 . T A . . . GT . . Pf3D7_02_v3 111 . T A . . . GT . . Pf3D7_02_v3 129 . T A . . . GT . . Pf3D7_02_v3 146 . A C,T . . . GT . . Pf3D7_02_v3 147 . A C,T . . . GT . . Pf3D7_02_v3 152 . G T . . . GT . . Pf3D7_02_v3 168 . T A . . . GT . . Pf3D7_02_v3 169 . A G . . . GT . . Pf3D7_02_v3 175 . A T . . . GT . . Pf3D7_02_v3 195 . T A . . . GT . . Pf3D7_02_v3 196 . A C . . . GT . . Pf3D7_02_v3 199 . T C . . . GT . . Pf3D7_02_v3 204 . C T . . . GT . . Pf3D7_02_v3 213 . G A . . . GT . . Pf3D7_02_v3 222 . A G . . . GT . . Pf3D7_02_v3 223 . A G . . . GT . . Pf3D7_02_v3 227 . T A . . . GT . . Pf3D7_02_v3 235 . A G . . . GT . . Pf3D7_03_v3 1 . A T . . . GT . . Pf3D7_03_v3 2 . 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T C 999 PASS DP4=13263,27466,40128,48208;DP=146673;Dels=0;FS=43.639;HWE=0.622715;ICF=-0.01176;MQ0=1;MQ=46;PV4=0.65,1,0,1;QD=14.81;AN=2;AC=1 GT:PL:DP:GQ 0:0,255:20:99 1:255,0:33:99 Y 8657215 . C A 999 PASS DP4=74915,114274,1948,2955;DP=195469;Dels=0;FS=3.181;MQ0=0;MQ=50;PV4=0.86,1,0,1;QD=33.77;AN=2;AC=1 GT:PL:DP:GQ 0:0,255:47:99 1:255,0:64:99 Y 10011673 rs78249411 G A 999 MinAB DP4=47351,30839,178796,279653;DP=550762;Dels=0;FS=41.028;MQ0=37362;MQ=26;PV4=0,0,0,1;QD=17.45;AN=2;AC=2 GT:PL:DP:GQ 1:126,101:146:37 1:95,0:130:99 bcftools-1.2/test/view.filter.1.out000066400000000000000000000005121246371514100172760ustar00rootroot000000000000001 3162007 . 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GAA G,GA 238 PASS DP=19;AN=4;AC=1,1;XRF=1e+06,2e+06,3e+06;XRI=1111,2222,3333;XRS=ABC,DEF,GHI;XAF=1e+06,2e+06;XAI=1111,2222;XAS=ABC,DEF;XGF=1e+06,2e+06,3e+06,4e+06,5e+06,6e+06;XGI=11,22,33,44,55,66;XGS=ABC,DEF,GHI,JKL,MNO,PQR;TXT=ABC,DEF,GHI GT:GQ:DP:STR 0/1:589:19:XX 0/2:1:1:YY 1 3162007 . TAGGG CAGGG,CAGGT 238 PASS AO=52101,113;CIGAR=1X4M,1X3M1X;TXT0=text GT:FGS:FGI:FGF:FAS:FAI:FAF:FRS:FRI:FRF ./.:AAAAAA,BBBBB,CCCC,DDD,EE,F:1,2,3,4,5,6:0.1,0.02,0.003,0.0004,5e-05,6e-06:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 2:AAAAAA,BBB,C:1,2,3:0.1,0.02,0.003:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 bcftools-1.2/test/view.filter.11.out000066400000000000000000000072521246371514100173670ustar00rootroot00000000000000##fileformat=VCFv4.2 ##FILTER= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##SAMPLE=\") quoting nonsense where double brackets would do just fine",softwareName=,softwareVer=<119,65>,softwareParam=<.>,MetadataResource=http://somewhere.com/path> ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3162006 . GAA G,GA 238 PASS DP=19;AN=2;AC=0,1;XRF=1e+06,2e+06,3e+06;XRI=1111,2222,3333;XRS=ABC,DEF,GHI;XAF=1e+06,2e+06;XAI=1111,2222;XAS=ABC,DEF;XGF=1e+06,2e+06,3e+06,4e+06,5e+06,6e+06;XGI=11,22,33,44,55,66;XGS=ABC,DEF,GHI,JKL,MNO,PQR;TXT=ABC,DEF,GHI GT:GQ:DP:STR ./.:589:19:XX 0/2:1:1:YY 1 3162007 . TAGGG CAGGG,CAGGT 238 PASS AO=52101,113;CIGAR=1X4M,1X3M1X;TXT0=text GT:FGS:FGI:FGF:FAS:FAI:FAF:FRS:FRI:FRF 0/1:AAAAAA,BBBBB,CCCC,DDD,EE,F:1,2,3,4,5,6:0.1,0.02,0.003,0.0004,5e-05,6e-06:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 2:AAAAAA,BBB,C:1,2,3:0.1,0.02,0.003:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 bcftools-1.2/test/view.filter.2.out000066400000000000000000000005121246371514100172770ustar00rootroot000000000000001 3162007 . TAGGG CAGGG,CAGGT 238 PASS AO=52101,113;CIGAR=1X4M,1X3M1X;TXT0=text GT:FGS:FGI:FGF:FAS:FAI:FAF:FRS:FRI:FRF 0/1:AAAAAA,BBBBB,CCCC,DDD,EE,F:1,2,3,4,5,6:0.1,0.02,0.003,0.0004,5e-05,6e-06:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 2:AAAAAA,BBB,C:1,2,3:0.1,0.02,0.003:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 bcftools-1.2/test/view.filter.3.out000066400000000000000000000005121246371514100173000ustar00rootroot000000000000001 3162007 . TAGGG CAGGG,CAGGT 238 PASS AO=52101,113;CIGAR=1X4M,1X3M1X;TXT0=text GT:FGS:FGI:FGF:FAS:FAI:FAF:FRS:FRI:FRF 0/1:AAAAAA,BBBBB,CCCC,DDD,EE,F:1,2,3,4,5,6:0.1,0.02,0.003,0.0004,5e-05,6e-06:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 2:AAAAAA,BBB,C:1,2,3:0.1,0.02,0.003:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 bcftools-1.2/test/view.filter.4.out000066400000000000000000000005121246371514100173010ustar00rootroot000000000000001 3162007 . TAGGG CAGGG,CAGGT 238 PASS AO=52101,113;CIGAR=1X4M,1X3M1X;TXT0=text GT:FGS:FGI:FGF:FAS:FAI:FAF:FRS:FRI:FRF 0/1:AAAAAA,BBBBB,CCCC,DDD,EE,F:1,2,3,4,5,6:0.1,0.02,0.003,0.0004,5e-05,6e-06:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 2:AAAAAA,BBB,C:1,2,3:0.1,0.02,0.003:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 bcftools-1.2/test/view.filter.5.out000066400000000000000000000005121246371514100173020ustar00rootroot000000000000001 3162007 . TAGGG CAGGG,CAGGT 238 PASS AO=52101,113;CIGAR=1X4M,1X3M1X;TXT0=text GT:FGS:FGI:FGF:FAS:FAI:FAF:FRS:FRI:FRF 0/1:AAAAAA,BBBBB,CCCC,DDD,EE,F:1,2,3,4,5,6:0.1,0.02,0.003,0.0004,5e-05,6e-06:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 2:AAAAAA,BBB,C:1,2,3:0.1,0.02,0.003:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 bcftools-1.2/test/view.filter.6.out000066400000000000000000000072521246371514100173130ustar00rootroot00000000000000##fileformat=VCFv4.2 ##FILTER= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##SAMPLE=\") quoting nonsense where double brackets would do just fine",softwareName=,softwareVer=<119,65>,softwareParam=<.>,MetadataResource=http://somewhere.com/path> ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3162006 . GAA G,GA 238 PASS DP=19;AN=4;AC=1,1;XRF=1e+06,2e+06,3e+06;XRI=1111,2222,3333;XRS=ABC,DEF,GHI;XAF=1e+06,2e+06;XAI=1111,2222;XAS=ABC,DEF;XGF=1e+06,2e+06,3e+06,4e+06,5e+06,6e+06;XGI=11,22,33,44,55,66;XGS=ABC,DEF,GHI,JKL,MNO,PQR;TXT=ABC,DEF,GHI GT:GQ:DP:STR 0/1:589:19:XX 0/2:1:1:YY 1 3162007 . TAGGG CAGGG,CAGGT 238 PASS AO=52101,113;CIGAR=1X4M,1X3M1X;TXT0=text GT:FGS:FGI:FGF:FAS:FAI:FAF:FRS:FRI:FRF ./.:AAAAAA,BBBBB,CCCC,DDD,EE,F:1,2,3,4,5,6:0.1,0.02,0.003,0.0004,5e-05,6e-06:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 .:AAAAAA,BBB,C:1,2,3:0.1,0.02,0.003:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 bcftools-1.2/test/view.filter.7.out000066400000000000000000000072521246371514100173140ustar00rootroot00000000000000##fileformat=VCFv4.2 ##FILTER= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##SAMPLE=\") quoting nonsense where double brackets would do just fine",softwareName=,softwareVer=<119,65>,softwareParam=<.>,MetadataResource=http://somewhere.com/path> ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3162006 . GAA G,GA 238 PASS DP=19;AN=4;AC=1,1;XRF=1e+06,2e+06,3e+06;XRI=1111,2222,3333;XRS=ABC,DEF,GHI;XAF=1e+06,2e+06;XAI=1111,2222;XAS=ABC,DEF;XGF=1e+06,2e+06,3e+06,4e+06,5e+06,6e+06;XGI=11,22,33,44,55,66;XGS=ABC,DEF,GHI,JKL,MNO,PQR;TXT=ABC,DEF,GHI GT:GQ:DP:STR 0/1:589:19:XX 0/2:1:1:YY 1 3162007 . TAGGG CAGGG,CAGGT 238 PASS AO=52101,113;CIGAR=1X4M,1X3M1X;TXT0=text GT:FGS:FGI:FGF:FAS:FAI:FAF:FRS:FRI:FRF ./.:AAAAAA,BBBBB,CCCC,DDD,EE,F:1,2,3,4,5,6:0.1,0.02,0.003,0.0004,5e-05,6e-06:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 .:AAAAAA,BBB,C:1,2,3:0.1,0.02,0.003:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 bcftools-1.2/test/view.filter.8.out000066400000000000000000000072521246371514100173150ustar00rootroot00000000000000##fileformat=VCFv4.2 ##FILTER= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##SAMPLE=\") quoting nonsense where double brackets would do just fine",softwareName=,softwareVer=<119,65>,softwareParam=<.>,MetadataResource=http://somewhere.com/path> ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3162006 . GAA G,GA 238 PASS DP=19;AN=4;AC=1,1;XRF=1e+06,2e+06,3e+06;XRI=1111,2222,3333;XRS=ABC,DEF,GHI;XAF=1e+06,2e+06;XAI=1111,2222;XAS=ABC,DEF;XGF=1e+06,2e+06,3e+06,4e+06,5e+06,6e+06;XGI=11,22,33,44,55,66;XGS=ABC,DEF,GHI,JKL,MNO,PQR;TXT=ABC,DEF,GHI GT:GQ:DP:STR 0/1:589:19:XX 0/2:1:1:YY 1 3162007 . TAGGG CAGGG,CAGGT 238 PASS AO=52101,113;CIGAR=1X4M,1X3M1X;TXT0=text GT:FGS:FGI:FGF:FAS:FAI:FAF:FRS:FRI:FRF ./.:AAAAAA,BBBBB,CCCC,DDD,EE,F:1,2,3,4,5,6:0.1,0.02,0.003,0.0004,5e-05,6e-06:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 .:AAAAAA,BBB,C:1,2,3:0.1,0.02,0.003:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 bcftools-1.2/test/view.filter.9.out000066400000000000000000000072521246371514100173160ustar00rootroot00000000000000##fileformat=VCFv4.2 ##FILTER= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##SAMPLE=\") quoting nonsense where double brackets would do just fine",softwareName=,softwareVer=<119,65>,softwareParam=<.>,MetadataResource=http://somewhere.com/path> ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3162006 . GAA G,GA 238 PASS DP=19;AN=4;AC=1,1;XRF=1e+06,2e+06,3e+06;XRI=1111,2222,3333;XRS=ABC,DEF,GHI;XAF=1e+06,2e+06;XAI=1111,2222;XAS=ABC,DEF;XGF=1e+06,2e+06,3e+06,4e+06,5e+06,6e+06;XGI=11,22,33,44,55,66;XGS=ABC,DEF,GHI,JKL,MNO,PQR;TXT=ABC,DEF,GHI GT:GQ:DP:STR 0/1:589:19:XX 0/2:1:1:YY 1 3162007 . TAGGG CAGGG,CAGGT 238 PASS AO=52101,113;CIGAR=1X4M,1X3M1X;TXT0=text GT:FGS:FGI:FGF:FAS:FAI:FAF:FRS:FRI:FRF 0/1:AAAAAA,BBBBB,CCCC,DDD,EE,F:1,2,3,4,5,6:0.1,0.02,0.003,0.0004,5e-05,6e-06:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 .:AAAAAA,BBB,C:1,2,3:0.1,0.02,0.003:AAA,B:1,2:0.1,0.02:A,BB,CCC:1,2,3:0.1,0.02,0.003 bcftools-1.2/test/view.filter.vcf000066400000000000000000000071361246371514100171170ustar00rootroot00000000000000##fileformat=VCFv4.2 ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##SAMPLE=\") quoting nonsense where double brackets would do just fine",softwareName=,softwareVer=<119,65>,softwareParam=<.>,MetadataResource=http://somewhere.com/path> ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3162006 . GAA G,GA 238 PASS DP=19;AN=4;AC=1,1;XRF=1e6,2e6,3e6;XRI=1111,2222,3333;XRS=ABC,DEF,GHI;XAF=1e6,2e6;XAI=1111,2222;XAS=ABC,DEF;XGF=1e6,2e6,3e6,4e6,5e6,6e6;XGI=11,22,33,44,55,66;XGS=ABC,DEF,GHI,JKL,MNO,PQR;TXT=ABC,DEF,GHI GT:GQ:DP:STR 0/1:589:19:XX 0/2:1:1:YY 1 3162007 . TAGGG CAGGG,CAGGT 238 PASS AO=52101,113;CIGAR=1X4M,1X3M1X;TXT0=text GT:FGS:FGI:FGF:FAS:FAI:FAF:FRS:FRI:FRF 0/1:AAAAAA,BBBBB,CCCC,DDD,EE,F:1,2,3,4,5,6:1e-1,2e-2,3e-3,4e-4,5e-5,6e-6:AAA,B:1,2:1e-1,2e-2:A,BB,CCC:1,2,3:1e-1,2e-2,3e-3 2:AAAAAA,BBB,C:1,2,3:1e-1,2e-2,3e-3:AAA,B:1,2:1e-1,2e-2:A,BB,CCC:1,2,3:1e-1,2e-2,3e-3 bcftools-1.2/test/view.minmaxac.1.out000066400000000000000000000001571246371514100176130ustar00rootroot0000000000000020 1234567 . A G,C,T 50 PASS NS=3;DP=9;AC=3,1,1;AN=8 GT:GQ:DP:TS 0/1:35:4:A,. 0/2:17:2:. 1/1:40:3:. 0/3:17:2:. bcftools-1.2/test/view.minmaxac.2.out000066400000000000000000000001571246371514100176140ustar00rootroot0000000000000020 1234568 . A G,C,T 50 PASS NS=3;DP=9;AC=2,2,2;AN=8 GT:GQ:DP:TS 0/1:35:4:A,. 2/2:17:2:. 3/3:40:3:. 0/1:17:2:. bcftools-1.2/test/view.minmaxac.vcf000066400000000000000000000016641246371514100174270ustar00rootroot00000000000000##fileformat=VCFv4.2 ##reference=file:///seq/references/1000GenomesPilot-NCBI36.fasta ##contig= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT NA00001 NA00002 NA00003 NA00004 20 1234567 . A G,C,T 50 PASS NS=3;DP=9 GT:GQ:DP:TS 0/1:35:4:A,. 0/2:17:2 1/1:40:3 0/3:17:2 20 1234568 . A G,C,T 50 PASS NS=3;DP=9 GT:GQ:DP:TS 0/1:35:4:A,. 2/2:17:2 3/3:40:3 0/1:17:2 bcftools-1.2/test/view.omitgenotypes.out000066400000000000000000000023571246371514100205710ustar00rootroot00000000000000##fileformat=VCFv4.2 ##FILTER= ##reference=file:///seq/references/1000GenomesPilot-NCBI36.fasta ##contig= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT NA00001 NA00002 NA00003 20 14370 rs6054257 G A 29 PASS NS=3;DP=14 GT:GQ:DP:HQ 0|0:48:1:51,51 1|0:48:8:51,51 1/1:43:5:.,. 20 17330 . T A 3 PASS NS=3;DP=11 GT:GQ:DP:HQ ./.:.:.:.,. ./.:.:.:.,. ./.:.:.:.,. 20 1110696 rs6040355 A G,T 67 PASS NS=2;DP=10 GT:GQ:DP:HQ:TS ./.:.:.:.:. ./.:.:.:.:. ./.:.:.:.:. 20 1230237 . T . 47 PASS NS=3;DP=13 GT:GQ:DP:HQ:TS .:.:.:.:. .:.:.:.:. .:.:.:.:. 20 1234567 microsat1 GTC G,GTCT 50 PASS NS=3;DP=9 GT:GQ:DP:TS 0/1:35:4:A,. 0/2:17:2:. 1/1:40:3:. bcftools-1.2/test/view.omitgenotypes.vcf000066400000000000000000000022071246371514100205320ustar00rootroot00000000000000##fileformat=VCFv4.2 ##reference=file:///seq/references/1000GenomesPilot-NCBI36.fasta ##contig= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT NA00001 NA00002 NA00003 20 14370 rs6054257 G A 29 PASS NS=3;DP=14 GT:GQ:DP:HQ 0|0:48:1:51,51 1|0:48:8:51,51 1/1:43:5:.,. 20 17330 . T A 3 PASS NS=3;DP=11 GT:GQ:DP:HQ ./.:.:.:.,. ./.:.:.:.,. ./.:.:.:.,. 20 1110696 rs6040355 A G,T 67 PASS NS=2;DP=10 GT:GQ:DP:HQ:TS ./. ./. ./. 20 1230237 . T . 47 PASS NS=3;DP=13 GT:GQ:DP:HQ:TS . . . 20 1234567 microsat1 GTC G,GTCT 50 PASS NS=3;DP=9 GT:GQ:DP:TS 0/1:35:4:A,. 0/2:17:2 1/1:40:3 bcftools-1.2/test/view.vcf000066400000000000000000000126021246371514100156250ustar00rootroot00000000000000##fileformat=VCFv4.1 ##reference=file:///seq/references/1000Genomes-NCBI37.fasta ##contig= ##contig= ##contig= ##contig= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##FILTER= ##FILTER= ##FILTER= ##FILTER= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT NA00001 NA00002 NA00003 11 2343543 . A . 999 PASS DP=100223 GT:PL:DP:GQ 0/0:0,255,255:193:99 0/0:0,255,255:211:99 0/0:0,255,255:182:99 11 5464562 . C T 999 PASS DP=0 GT:PL:DP:GQ ./.:0,0,0:.:. ./.:0,0,0:.:. ./.:0,0,0:.:. 20 76962 rs6111385 T C 999 PASS DP4=110138,70822,421911,262673;DP=911531;Dels=0;FS=21.447;HWE=0.491006;ICF=-0.01062;MQ0=1;MQ=46;PV4=2.5e-09,0,0,1;QD=22.31 GT:PL:DP:GQ 0/1:255,0,255:193:99 1/1:255,255,0:211:99 1/1:255,255,0:182:99 20 126310 . ACC A 999 StrandBias;EndDistBias DP4=125718,95950,113812,80890;DP=461867;HWE=0.24036;ICF=0.01738;INDEL;IS=374,0.937343;MQ=49;PV4=9e-30,1,0,3.8e-13;QD=0.0172;AN=6;AC=4 GT:DP:GQ:PL 0/1:117:99:255,0,132 0/1:111:99:255,0,139 1/1:78:99:255,213,0 20 138125 rs2298108 G T 999 PASS DP4=174391,20849,82080,4950;DP=286107;Dels=0;FS=3200;HWE=0.199462;ICF=0.01858;MQ0=0;MQ=46;PV4=0,0,0,1;QD=17.22;AN=6;AC=4 GT:PL:DP:GQ 0/1:135,0,163:66:99 0/1:140,0,255:71:99 1/1:255,199,0:66:99 20 138148 rs2298109 C T 999 PASS DP4=194136,45753,94945,14367;DP=356657;Dels=0;FS=3200;HWE=0.177865;ICF=0.0198;MQ0=0;MQ=47;PV4=0,0,0,1;QD=14.57;AN=6;AC=4 GT:PL:DP:GQ 0/1:195,0,255:87:99 0/1:192,0,255:82:99 1/1:255,235,0:78:99 20 271225 . T TTTA,TA 999 StrandBias DP4=29281,42401,27887,29245;DP=272732;INDEL;IS=95,0.748031;MQ=47;PV4=0,1,0,1;QD=0.0948;AN=6;AC=2,2 GT:DP:GQ:PL 0/2:33:49:151,53,203,0,52,159 0/1:51:99:255,0,213,255,255,255 1/2:47:99:255,255,255,255,0,241 20 304568 . C T 999 PASS DP4=16413,4543,945,156;DP=43557;Dels=0;FS=3200;HWE=0.076855;ICF=0.0213;MQ0=0;MQ=50;PV4=0,0,0,1;QD=15.45;AN=6;AC=4 GT:PL:DP:GQ 0|1:95,0,255:90:99 0|1:192,0,255:13:99 1|1:255,95,0:60:99 20 326891 . A AC 999 PASS DP4=125718,95950,113812,80890;DP=461867;HWE=0.24036;ICF=0.01738;INDEL;IS=374,0.937343;MQ=49;PV4=9e-30,1,0,3.8e-13;QD=0.0172;AN=4;AC=2 GT:DP:GQ:PL 0|1:117:99:255,0,132 0|1:111:99:255,0,139 ./.:.:.:.,.,. X 2928329 rs62584840 C T 999 PASS DP4=302,9137,32,1329;DP=11020;Dels=0;FS=13.38;HWE=0.284332;ICF=0.0253;MQ0=0;MQ=49;PV4=0.094,0,0,1;QD=18.61;AN=4;AC=1 GT:PL:DP:GQ 0:0,56:2:73 0:0,81:3:98 0/1:73,0,19:4:30 X 2933066 rs61746890 G C 999 PASS DP4=69865,100561,461,783;DP=173729;Dels=0;FS=10.833;MQ0=0;MQ=50;PV4=0.005,3.6e-14,0,1;QD=15.33;AN=4;AC=1 GT:PL:DP:GQ 0:0,255:39:99 0:0,255:37:99 0/1:255,255,255:62:99 X 2942109 rs5939407 T C 999 PASS DP4=23273,27816,40128,48208;DP=146673;Dels=0;FS=43.639;HWE=0.622715;ICF=-0.01176;MQ0=1;MQ=46;PV4=0.65,1,0,1;QD=14.81;AN=4;AC=3 GT:PL:DP:GQ 0:0,255:20:99 1:255,0:33:99 1/1:255,157,0:52:99 X 3048719 . T C 999 PASS DP4=13263,27466,40128,48208;DP=146673;Dels=0;FS=43.639;HWE=0.622715;ICF=-0.01176;MQ0=1;MQ=46;PV4=0.65,1,0,1;QD=14.81;AN=4;AC=3 GT:PL:DP:GQ 0:0,255:20:99 1:255,0:33:99 0|1:255,0,157:52:99 Y 8657215 . C A 999 PASS DP4=74915,114274,1948,2955;DP=195469;Dels=0;FS=3.181;MQ0=0;MQ=50;PV4=0.86,1,0,1;QD=33.77;AN=2;AC=1 GT:PL:DP:GQ 0:0,255:47:99 1:255,0:64:99 . Y 10011673 rs78249411 G A 999 MinAB DP4=47351,30839,178796,279653;DP=550762;Dels=0;FS=41.028;MQ0=37362;MQ=26;PV4=0,0,0,1;QD=17.45;AN=2;AC=2 GT:PL:DP:GQ 1:126,101:146:37 1:95,0:130:99 . bcftools-1.2/test/view.vectors.A.out000066400000000000000000000105341246371514100175230ustar00rootroot00000000000000##fileformat=VCFv4.2 ##FILTER= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##SAMPLE=\") quoting nonsense where double brackets would do just fine",softwareName=,softwareVer=<119,65>,softwareParam=<.>,MetadataResource=http://somewhere.com/path> ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A 1 3162006 . GAA G 238 PASS DP=19;AN=2;AC=1;XRF=1e+06,2e+06;XRI=1111,2222;XRS=ABC,DEF;XAF=1e+06;XAI=1111;XAS=ABC;XGF=1e+06,2e+06,3e+06;XGI=11,22,33;XGS=ABC,DEF,GHI;TXT=ABC,DEF,GHI GT:GQ:DP 0/1:589:19 1 3162007 . TAGGG CAGGG 238 PASS AO=52101;CIGAR=1X4M;AC=1;AN=2 GT:FGS:FGI:FGF:FAS:FAI:FAF:FRS:FRI:FRF 0/1:AAAAAA,BBBBB,CCCC:1,2,3:0.1,0.02,0.003:AAA:1:0.1:A,BB:1,2:0.1,0.02 1 3162008 . TAGGG CAGGG 238 PASS IA8=10,.;IA16=1000,.;IA32=100000,.;IAF=0.003,.;CIGAR=1X4M;AC=1;AN=2 GT:F8:F16:F32:FF 0/1:10:1000:100000:0.003 1 3162009 . TAGGG CAGGG 238 PASS IA8=.;IA16=.;IA32=.;IAF=.;CIGAR=1X4M;AC=1;AN=2 GT:F8:F16:F32:FF 0/1:10:1000:100000:0.003 1 3162010 . TAGGG CAGGG 238 PASS I8=.;I16=.;I32=.;IF=.;CIGAR=1X4M;AC=1;AN=2 GT:F8:F16:F32:FF 0/1:10:1000:100000:0.003 bcftools-1.2/test/view.vectors.B.out000066400000000000000000000104421246371514100175220ustar00rootroot00000000000000##fileformat=VCFv4.2 ##FILTER= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##SAMPLE=\") quoting nonsense where double brackets would do just fine",softwareName=,softwareVer=<119,65>,softwareParam=<.>,MetadataResource=http://somewhere.com/path> ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT B 1 3162006 . GAA GA 238 PASS DP=19;AN=2;AC=1;XRF=1e+06,3e+06;XRI=1111,3333;XRS=ABC,GHI;XAF=2e+06;XAI=2222;XAS=DEF;XGF=1e+06,4e+06,6e+06;XGI=11,44,66;XGS=ABC,JKL,PQR;TXT=ABC,DEF,GHI GT:GQ:DP 0/1:1:1 1 3162007 . TAGGG CAGGT 238 PASS AO=113;CIGAR=1X3M1X;AC=1;AN=1 GT:FGS:FGI:FGF:FAS:FAI:FAF:FRS:FRI:FRF 1:AAAAAA,C:1,3:0.1,0.003:B:2:0.02:A,CCC:1,3:0.1,0.003 1 3162008 . TAGGG CAGGT 238 PASS IA8=10,.;IA16=1000,.;IA32=100000,.;IAF=0.003,.;CIGAR=1X3M1X;AC=1;AN=1 GT:F8:F16:F32:FF 1:.:.:.:. 1 3162009 . TAGGG CAGGT 238 PASS IA8=.;IA16=.;IA32=.;IAF=.;CIGAR=1X3M1X;AC=1;AN=1 GT:F8:F16:F32:FF 1:.:.:.:. 1 3162010 . TAGGG CAGGT 238 PASS I8=.;I16=.;I32=.;IF=.;CIGAR=1X3M1X;AC=1;AN=1 GT:F8:F16:F32:FF 1:.:.:.:. bcftools-1.2/test/view.vectors.vcf000066400000000000000000000110321246371514100173050ustar00rootroot00000000000000##fileformat=VCFv4.2 ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##SAMPLE=\") quoting nonsense where double brackets would do just fine",softwareName=,softwareVer=<119,65>,softwareParam=<.>,MetadataResource=http://somewhere.com/path> ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= ##FILTER= ##contig= ##contig= ##contig= ##contig= ##reference=file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##INFO= ##FORMAT= ##FORMAT= ##FORMAT= ##FORMAT= #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT A B 1 3162006 . GAA G,GA 238 PASS DP=19;AN=4;AC=1,1;XRF=1e6,2e6,3e6;XRI=1111,2222,3333;XRS=ABC,DEF,GHI;XAF=1e6,2e6;XAI=1111,2222;XAS=ABC,DEF;XGF=1e6,2e6,3e6,4e6,5e6,6e6;XGI=11,22,33,44,55,66;XGS=ABC,DEF,GHI,JKL,MNO,PQR;TXT=ABC,DEF,GHI GT:GQ:DP 0/1:589:19 0/2:1:1 1 3162007 . TAGGG CAGGG,CAGGT 238 PASS AO=52101,113;CIGAR=1X4M,1X3M1X GT:FGS:FGI:FGF:FAS:FAI:FAF:FRS:FRI:FRF 0/1:AAAAAA,BBBBB,CCCC,DDD,EE,F:1,2,3,4,5,6:1e-1,2e-2,3e-3,4e-4,5e-5,6e-6:AAA,B:1,2:1e-1,2e-2:A,BB,CCC:1,2,3:1e-1,2e-2,3e-3 2:AAAAAA,BBB,C:1,2,3:1e-1,2e-2,3e-3:AAA,B:1,2:1e-1,2e-2:A,BB,CCC:1,2,3:1e-1,2e-2,3e-3 1 3162008 . TAGGG CAGGG,CAGGT 238 PASS IA8=10,.;IA16=1000,.;IA32=100000,.;IAF=3e-3,.;CIGAR=1X4M,1X3M1X GT:F8:F16:F32:FF 0/1:10:1000:100000:3e-3 2:.:.:.:. 1 3162009 . TAGGG CAGGG,CAGGT 238 PASS IA8=.;IA16=.;IA32=.;IAF=.;CIGAR=1X4M,1X3M1X GT:F8:F16:F32:FF 0/1:10:1000:100000:3e-3 2:.:.:.:. 1 3162010 . TAGGG CAGGG,CAGGT 238 PASS I8=.;I16=.;I32=.;IF=.;CIGAR=1X4M,1X3M1X GT:F8:F16:F32:FF 0/1:10:1000:100000:3e-3 2:.:.:.:. bcftools-1.2/tsv2vcf.c000066400000000000000000000070231246371514100147360ustar00rootroot00000000000000/* tsv2vcf.c -- convert from whitespace-separated fields to VCF Copyright (C) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include "tsv2vcf.h" tsv_t *tsv_init(const char *str) { tsv_t *tsv = (tsv_t *) calloc(1,sizeof(tsv_t)); kstring_t tmp = {0,0,0}; const char *ss = str, *se = ss; tsv->ncols = 0; while ( *ss ) { if ( *se && *se!=',' ) { se++; continue; } tsv->ncols++; tsv->cols = (tsv_col_t*) realloc(tsv->cols,sizeof(tsv_col_t)*tsv->ncols); tsv->cols[tsv->ncols-1].name = NULL; tsv->cols[tsv->ncols-1].setter = NULL; tmp.l = 0; kputsn(ss, se-ss, &tmp); if ( strcasecmp("-",tmp.s) ) tsv->cols[tsv->ncols-1].name = strdup(tmp.s); if ( !*se ) break; ss = ++se; } free(tmp.s); return tsv; } void tsv_destroy(tsv_t *tsv) { int i; for (i=0; incols; i++) free(tsv->cols[i].name); free(tsv->cols); free(tsv); } int tsv_register(tsv_t *tsv, const char *id, tsv_setter_t setter, void *usr) { int i; for (i=0; incols; i++) { if ( !tsv->cols[i].name || strcasecmp(tsv->cols[i].name,id) ) continue; tsv->cols[i].setter = setter; tsv->cols[i].usr = usr; return 0; } return -1; } int tsv_parse(tsv_t *tsv, bcf1_t *rec, char *str) { int status = 0; tsv->icol = 0; tsv->ss = tsv->se = str; while ( *tsv->ss && tsv->icol < tsv->ncols ) { while ( *tsv->se && !isspace(*tsv->se) ) tsv->se++; if ( tsv->cols[tsv->icol].setter ) { int ret = tsv->cols[tsv->icol].setter(tsv,rec,tsv->cols[tsv->icol].usr); if ( ret<0 ) return -1; status++; } while ( *tsv->se && isspace(*tsv->se) ) tsv->se++; tsv->ss = tsv->se; tsv->icol++; } return status ? 0 : -1; } int tsv_setter_chrom(tsv_t *tsv, bcf1_t *rec, void *usr) { char tmp = *tsv->se; *tsv->se = 0; rec->rid = bcf_hdr_name2id((bcf_hdr_t*)usr, tsv->ss); *tsv->se = tmp; return rec->rid==-1 ? -1 : 0; } int tsv_setter_pos(tsv_t *tsv, bcf1_t *rec, void *usr) { char *endptr; rec->pos = strtol(tsv->ss, &endptr, 10) - 1; if ( tsv->ss==endptr ) return -1; return 0; } int tsv_setter_id(tsv_t *tsv, bcf1_t *rec, void *usr) { char tmp = *tsv->se; *tsv->se = 0; bcf_update_id((bcf_hdr_t*)usr, rec, tsv->ss); *tsv->se = tmp; return 0; } bcftools-1.2/tsv2vcf.h000066400000000000000000000050751246371514100147500ustar00rootroot00000000000000/* tsv2vcf.h -- convert from whitespace-separated fields to VCF Copyright (C) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #ifndef __TSV2VCF_H__ #define __TSV2VCF_H__ #include typedef struct _tsv_t tsv_t; typedef int (*tsv_setter_t)(tsv_t *, bcf1_t *, void *); typedef struct { char *name; tsv_setter_t setter; void *usr; } tsv_col_t; struct _tsv_t { int ncols, icol; tsv_col_t *cols; char *se, *ss; }; tsv_t *tsv_init(const char *str); void tsv_destroy(tsv_t *tsv); int tsv_register(tsv_t *tsv, const char *id, tsv_setter_t setter, void *usr); /** * tsv_parse() - parse tsv line and fill VCF record * Returns 0 on success or -1 on parse error */ int tsv_parse(tsv_t *tsv, bcf1_t *rec, char *str); /** * tstv_next() - position ss,se to next field; first pass with ss=se=str * Returns 0 on success, or -1 if no more fields */ static inline int tsv_next(tsv_t *tsv) { if ( !*tsv->se ) return -1; if ( tsv->ss==tsv->se ) { while ( *tsv->se && !isspace(*tsv->se) ) tsv->se++; return 0; } while ( *tsv->se && isspace(*tsv->se) ) tsv->se++; tsv->ss = tsv->se; while ( *tsv->se && !isspace(*tsv->se) ) tsv->se++; return 0; } /** * The setters return 0 on success or negative value if the line is to be skipped. */ int tsv_setter_chrom(tsv_t *tsv, bcf1_t *rec, void *usr); int tsv_setter_pos(tsv_t *tsv, bcf1_t *rec, void *usr); int tsv_setter_id(tsv_t *tsv, bcf1_t *rec, void *usr); #endif bcftools-1.2/vcfannotate.c000066400000000000000000002116441246371514100156570ustar00rootroot00000000000000/* vcfannotate.c -- Annotate and edit VCF/BCF files. Copyright (C) 2013-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include #include #include #include #include #include #include "bcftools.h" #include "vcmp.h" #include "filter.h" #include "convert.h" struct _args_t; typedef struct _rm_tag_t { char *key; int hdr_id; void (*handler)(struct _args_t *, bcf1_t *, struct _rm_tag_t *); } rm_tag_t; typedef struct { char **cols; int ncols, mcols; char **als; int nals, mals; kstring_t line; int rid, start, end; } annot_line_t; #define REPLACE_MISSING 0 // replace only missing values #define REPLACE_ALL 1 // replace both missing and existing values #define REPLACE_EXISTING 2 // replace only if tgt is not missing typedef struct _annot_col_t { int icol, replace, number; // number: one of BCF_VL_* types char *hdr_key; int (*setter)(struct _args_t *, bcf1_t *, struct _annot_col_t *, void*); } annot_col_t; // Logic of the filters: include or exclude sites which match the filters? #define FLT_INCLUDE 1 #define FLT_EXCLUDE 2 typedef struct _args_t { bcf_srs_t *files; bcf_hdr_t *hdr, *hdr_out; htsFile *out_fh; int output_type; bcf_sr_regions_t *tgts; filter_t *filter; char *filter_str; int filter_logic; // include or exclude sites which match the filters? One of FLT_INCLUDE/FLT_EXCLUDE rm_tag_t *rm; // tags scheduled for removal int nrm; int flt_keep_pass; // when all filters removed, reset to PASS vcmp_t *vcmp; // for matching annotation and VCF lines by allele annot_line_t *alines; // buffered annotation lines int nalines, malines; int ref_idx, alt_idx, chr_idx, from_idx, to_idx; // -1 if not present annot_col_t *cols; // column indexes and setters int ncols; char *set_ids_fmt; convert_t *set_ids; int set_ids_replace; int *sample_map, nsample_map, sample_is_file; // map[idst] -> isrc int mtmpi, mtmpf, mtmps; int mtmpi2, mtmpf2, mtmps2; int mtmpi3, mtmpf3, mtmps3; int32_t *tmpi, *tmpi2, *tmpi3; float *tmpf, *tmpf2, *tmpf3; char *tmps, *tmps2, **tmpp, **tmpp2; kstring_t tmpks; char **argv, *output_fname, *targets_fname, *regions_list, *header_fname; char *remove_annots, *columns, *rename_chrs, *sample_names; int argc, drop_header, tgts_is_vcf; } args_t; char *msprintf(const char *fmt, ...); void remove_id(args_t *args, bcf1_t *line, rm_tag_t *tag) { bcf_update_id(args->hdr,line,NULL); } void remove_filter(args_t *args, bcf1_t *line, rm_tag_t *tag) { if ( !tag->key ) bcf_update_filter(args->hdr, line, NULL, args->flt_keep_pass); else bcf_remove_filter(args->hdr, line, tag->hdr_id, args->flt_keep_pass); } void remove_qual(args_t *args, bcf1_t *line, rm_tag_t *tag) { bcf_float_set_missing(line->qual); } void remove_info(args_t *args, bcf1_t *line, rm_tag_t *tag) { // remove all INFO fields if ( !(line->unpacked & BCF_UN_INFO) ) bcf_unpack(line, BCF_UN_INFO); int i; for (i=0; in_info; i++) { bcf_info_t *inf = &line->d.info[i]; if ( inf->vptr_free ) { free(inf->vptr - inf->vptr_off); inf->vptr_free = 0; } line->d.shared_dirty |= BCF1_DIRTY_INF; inf->vptr = NULL; } } void remove_info_tag(args_t *args, bcf1_t *line, rm_tag_t *tag) { bcf_update_info(args->hdr, line, tag->key, NULL, 0, BCF_HT_INT); // the type does not matter with n=0 } void remove_format_tag(args_t *args, bcf1_t *line, rm_tag_t *tag) { bcf_update_format(args->hdr, line, tag->key, NULL, 0, BCF_HT_INT); // the type does not matter with n=0 } void remove_format(args_t *args, bcf1_t *line, rm_tag_t *tag) { // remove all FORMAT fields except GT if ( !(line->unpacked & BCF_UN_FMT) ) bcf_unpack(line, BCF_UN_FMT); int i; for (i=0; in_fmt; i++) { bcf_fmt_t *fmt = &line->d.fmt[i]; const char *key = bcf_hdr_int2id(args->hdr,BCF_DT_ID,fmt->id); if ( key[0]=='G' && key[1]=='T' && !key[2] ) continue; if ( fmt->p_free ) { free(fmt->p - fmt->p_off); fmt->p_free = 0; } line->d.indiv_dirty = 1; fmt->p = NULL; } } static void remove_hdr_lines(bcf_hdr_t *hdr, int type) { int i = 0, nrm = 0; while ( inhrec ) { if ( hdr->hrec[i]->type!=type ) { i++; continue; } bcf_hrec_t *hrec = hdr->hrec[i]; if ( type==BCF_HL_FMT ) { // everything except FORMAT/GT int id = bcf_hrec_find_key(hrec, "ID"); if ( id>=0 && !strcmp(hrec->vals[id],"GT") ) { i++; continue; } } nrm++; hdr->nhrec--; if ( i < hdr->nhrec ) memmove(&hdr->hrec[i],&hdr->hrec[i+1],(hdr->nhrec-i)*sizeof(bcf_hrec_t*)); bcf_hrec_destroy(hrec); } if ( nrm ) bcf_hdr_sync(hdr); } static void init_remove_annots(args_t *args) { int keep_info = 0, keep_fmt = 0, keep_flt = 0; void *keep = khash_str2int_init(); kstring_t str = {0,0,0}; char *ss = args->remove_annots; while ( *ss ) { args->nrm++; args->rm = (rm_tag_t*) realloc(args->rm,sizeof(rm_tag_t)*args->nrm); rm_tag_t *tag = &args->rm[args->nrm-1]; tag->key = NULL; int type = BCF_HL_GEN; if ( !strncasecmp("INFO/",ss,5) ) { type = BCF_HL_INFO; ss += 5; } else if ( !strncasecmp("INF/",ss,4) ) { type = BCF_HL_INFO; ss += 4; } else if ( !strncasecmp("FORMAT/",ss,7) ) { type = BCF_HL_FMT; ss += 7; } else if ( !strncasecmp("FMT/",ss,4) ) { type = BCF_HL_FMT; ss += 4; } else if ( !strncasecmp("FILTER/",ss,7) ) { type = BCF_HL_FLT; ss += 7; } else if ( !strncasecmp("^INFO/",ss,6) ) { type = BCF_HL_INFO; ss += 6; keep_info = 1; } else if ( !strncasecmp("^INF/",ss,5) ) { type = BCF_HL_INFO; ss += 5; keep_info = 1; } else if ( !strncasecmp("^FORMAT/",ss,8) ) { type = BCF_HL_FMT; ss += 8; keep_fmt = 1; } else if ( !strncasecmp("^FMT/",ss,5) ) { type = BCF_HL_FMT; ss += 5; keep_fmt = 1; } else if ( !strncasecmp("^FILTER/",ss,8) ) { type = BCF_HL_FLT; ss += 8; keep_flt = 1; } char *se = ss; while ( *se && *se!=',' ) se++; str.l = 0; kputsn(ss, se-ss, &str); if ( type==BCF_HL_FLT ) { if ( !keep_flt ) { args->flt_keep_pass = 1; tag->handler = remove_filter; tag->key = strdup(str.s); tag->hdr_id = bcf_hdr_id2int(args->hdr, BCF_DT_ID, tag->key); if ( !bcf_hdr_idinfo_exists(args->hdr,BCF_HL_FLT,tag->hdr_id) ) error("Cannot remove %s, not defined in the header.\n", str.s); bcf_hdr_remove(args->hdr_out,BCF_HL_FLT,tag->key); } else { int value, ret = khash_str2int_get(keep, str.s, &value); if ( ret==-1 ) khash_str2int_set(keep, strdup(str.s),1<nrm--; } } else if ( type!=BCF_HL_GEN ) { int id = bcf_hdr_id2int(args->hdr,BCF_DT_ID,str.s); if ( !bcf_hdr_idinfo_exists(args->hdr,type,id) ) { fprintf(stderr,"Warning: The tag \"%s\" not defined in the header\n", str.s); args->nrm--; } else if ( (type==BCF_HL_FMT && keep_fmt) || (type==BCF_HL_INFO && keep_info) ) { int value, ret = khash_str2int_get(keep, str.s, &value); if ( ret==-1 ) khash_str2int_set(keep, strdup(str.s),1<nrm--; } else { tag->key = strdup(str.s); if ( type==BCF_HL_INFO ) tag->handler = remove_info_tag; else if ( type==BCF_HL_FMT ) tag->handler = remove_format_tag; bcf_hdr_remove(args->hdr_out,type,tag->key); } } else if ( !strcasecmp("ID",str.s) ) tag->handler = remove_id; else if ( !strcasecmp("FILTER",str.s) ) { tag->handler = remove_filter; remove_hdr_lines(args->hdr_out,BCF_HL_FLT); } else if ( !strcasecmp("QUAL",str.s) ) tag->handler = remove_qual; else if ( !strcasecmp("INFO",str.s) ) { tag->handler = remove_info; remove_hdr_lines(args->hdr_out,BCF_HL_INFO); } else if ( !strcasecmp("FMT",str.s) || !strcasecmp("FORMAT",str.s) ) { tag->handler = remove_format; remove_hdr_lines(args->hdr_out,BCF_HL_FMT); } else if ( str.l ) { if ( str.s[0]=='#' && str.s[1]=='#' ) bcf_hdr_remove(args->hdr_out,BCF_HL_GEN,str.s+2); else bcf_hdr_remove(args->hdr_out,BCF_HL_STR,str.s); args->nrm--; } ss = *se ? se+1 : se; } free(str.s); if ( keep_flt || keep_info || keep_flt ) { int j; for (j=0; jhdr->nhrec; j++) { bcf_hrec_t *hrec = args->hdr->hrec[j]; if ( hrec->type!=BCF_HL_FLT && hrec->type!=BCF_HL_INFO && hrec->type!=BCF_HL_FMT ) continue; if ( !keep_flt && hrec->type==BCF_HL_FLT ) continue; if ( !keep_info && hrec->type==BCF_HL_INFO ) continue; if ( !keep_fmt && hrec->type==BCF_HL_FMT ) continue; int k = bcf_hrec_find_key(hrec,"ID"); assert( k>=0 ); // this should always be true for valid VCFs int value, ret = khash_str2int_get(keep,hrec->vals[k],&value); if ( ret==0 && value>>hrec->type ) // keep { if ( hrec->type==BCF_HL_FLT && !strcmp("PASS",hrec->vals[k]) ) args->flt_keep_pass = 1; continue; } args->nrm++; args->rm = (rm_tag_t*) realloc(args->rm,sizeof(rm_tag_t)*args->nrm); rm_tag_t *tag = &args->rm[args->nrm-1]; if ( hrec->type==BCF_HL_INFO ) tag->handler = remove_info_tag; else if ( hrec->type==BCF_HL_FMT ) tag->handler = remove_format_tag; else { tag->handler = remove_filter; tag->hdr_id = bcf_hdr_id2int(args->hdr, BCF_DT_ID, hrec->vals[k]); } tag->key = strdup(hrec->vals[k]); bcf_hdr_remove(args->hdr_out,hrec->type,tag->key); } } khash_str2int_destroy_free(keep); if ( !args->nrm ) error("No matching tag in -x %s\n", args->remove_annots); bcf_hdr_sync(args->hdr_out); } static void init_header_lines(args_t *args) { htsFile *file = hts_open(args->header_fname, "rb"); if ( !file ) error("Error reading %s\n", args->header_fname); kstring_t str = {0,0,0}; while ( hts_getline(file, KS_SEP_LINE, &str) > 0 ) { if ( bcf_hdr_append(args->hdr_out,str.s) ) error("Could not parse %s: %s\n", args->header_fname, str.s); bcf_hdr_append(args->hdr,str.s); // the input file may not have the header line if run with -h (and nothing else) } hts_close(file); free(str.s); bcf_hdr_sync(args->hdr_out); bcf_hdr_sync(args->hdr); } static int setter_filter(args_t *args, bcf1_t *line, annot_col_t *col, void *data) { annot_line_t *tab = (annot_line_t*) data; hts_expand(int,1,args->mtmpi,args->tmpi); args->tmpi[0] = bcf_hdr_id2int(args->hdr_out, BCF_DT_ID, tab->cols[col->icol]); if ( args->tmpi[0]<0 ) error("The FILTER is not defined in the header: %s\n", tab->cols[col->icol]); if ( col->replace!=REPLACE_MISSING ) bcf_update_filter(args->hdr_out,line,args->tmpi,1); else bcf_add_filter(args->hdr_out,line,args->tmpi[0]); return 0; } static int vcf_setter_filter(args_t *args, bcf1_t *line, annot_col_t *col, void *data) { bcf1_t *rec = (bcf1_t*) data; if ( !(rec->unpacked & BCF_UN_FLT) ) bcf_unpack(rec, BCF_UN_FLT); hts_expand(int,rec->d.n_flt,args->mtmpi,args->tmpi); int i; if ( col->replace!=REPLACE_MISSING ) { for (i=0; id.n_flt; i++) { const char *flt = bcf_hdr_int2id(args->files->readers[1].header, BCF_DT_ID, rec->d.flt[i]); args->tmpi[i] = bcf_hdr_id2int(args->hdr_out, BCF_DT_ID, flt); } bcf_update_filter(args->hdr_out,line,args->tmpi,rec->d.n_flt); return 0; } else { for (i=0; id.n_flt; i++) { const char *flt = bcf_hdr_int2id(args->files->readers[1].header, BCF_DT_ID, rec->d.flt[i]); bcf_add_filter(args->hdr_out,line,bcf_hdr_id2int(args->hdr_out, BCF_DT_ID, flt)); } return 0; } } static int setter_id(args_t *args, bcf1_t *line, annot_col_t *col, void *data) { // possible cases: // IN ANNOT OUT ACHIEVED_BY // x y x -c +ID // x y y -c ID // x y x,y /not supported/ // x . x -c +ID, ID // x . . -x ID // . y y -c +ID, -c ID // annot_line_t *tab = (annot_line_t*) data; if ( tab->cols[col->icol] && tab->cols[col->icol][0]=='.' && !tab->cols[col->icol][1] ) return 0; // don't replace with "." if ( col->replace!=REPLACE_MISSING ) return bcf_update_id(args->hdr_out,line,tab->cols[col->icol]); // running with +ID, only update missing ids if ( !line->d.id || (line->d.id[0]=='.' && !line->d.id[1]) ) return bcf_update_id(args->hdr_out,line,tab->cols[col->icol]); return 0; } static int vcf_setter_id(args_t *args, bcf1_t *line, annot_col_t *col, void *data) { bcf1_t *rec = (bcf1_t*) data; if ( rec->d.id && rec->d.id[0]=='.' && !rec->d.id[1] ) return 0; // don't replace with "." if ( col->replace!=REPLACE_MISSING ) return bcf_update_id(args->hdr_out,line,rec->d.id); // running with +ID, only update missing ids if ( !line->d.id || (line->d.id[0]=='.' && !line->d.id[1]) ) return bcf_update_id(args->hdr_out,line,rec->d.id); return 0; } static int setter_qual(args_t *args, bcf1_t *line, annot_col_t *col, void *data) { annot_line_t *tab = (annot_line_t*) data; char *str = tab->cols[col->icol]; if ( str[0]=='.' && str[1]==0 ) return 0; // empty if ( col->replace==REPLACE_MISSING && !bcf_float_is_missing(line->qual) ) return 0; line->qual = strtod(str, &str); if ( str == tab->cols[col->icol] ) error("Could not parse %s at %s:%d .. [%s]\n", col->hdr_key,bcf_seqname(args->hdr,line),line->pos+1,tab->cols[col->icol]); return 0; } static int vcf_setter_qual(args_t *args, bcf1_t *line, annot_col_t *col, void *data) { bcf1_t *rec = (bcf1_t*) data; if ( bcf_float_is_missing(rec->qual) ) return 0; if ( col->replace==REPLACE_MISSING && !bcf_float_is_missing(line->qual) ) return 0; line->qual = rec->qual; return 0; } static int setter_info_flag(args_t *args, bcf1_t *line, annot_col_t *col, void *data) { annot_line_t *tab = (annot_line_t*) data; char *str = tab->cols[col->icol]; if ( str[0]=='.' && str[1]==0 ) return 0; if ( str[0]=='1' && str[1]==0 ) return bcf_update_info_flag(args->hdr_out,line,col->hdr_key,NULL,1); if ( str[0]=='0' && str[1]==0 ) return bcf_update_info_flag(args->hdr_out,line,col->hdr_key,NULL,0); error("Could not parse %s at %s:%d .. [%s]\n", bcf_seqname(args->hdr,line),line->pos+1,tab->cols[col->icol]); return -1; } static int vcf_setter_info_flag(args_t *args, bcf1_t *line, annot_col_t *col, void *data) { bcf1_t *rec = (bcf1_t*) data; int flag = bcf_get_info_flag(args->files->readers[1].header,rec,col->hdr_key,NULL,NULL); bcf_update_info_flag(args->hdr_out,line,col->hdr_key,NULL,flag); return 0; } static int setter_ARinfo_int32(args_t *args, bcf1_t *line, annot_col_t *col, int nals, char **als, int ntmpi) { if ( col->number==BCF_VL_A && ntmpi!=nals-1 && (ntmpi!=1 || args->tmpi[0]!=bcf_int32_missing || args->tmpi[1]!=bcf_int32_vector_end) ) error("Incorrect number of values (%d) for the %s tag at %s:%d\n", ntmpi,col->hdr_key,bcf_seqname(args->hdr,line),line->pos+1); else if ( col->number==BCF_VL_R && ntmpi!=nals && (ntmpi!=1 || args->tmpi[0]!=bcf_int32_missing || args->tmpi[1]!=bcf_int32_vector_end) ) error("Incorrect number of values (%d) for the %s tag at %s:%d\n", ntmpi,col->hdr_key,bcf_seqname(args->hdr,line),line->pos+1); int ndst = col->number==BCF_VL_A ? line->n_allele - 1 : line->n_allele; int *map = vcmp_map_ARvalues(args->vcmp,ndst,nals,als,line->n_allele,line->d.allele); if ( !map ) error("REF alleles not compatible at %s:%d\n"); // fill in any missing values in the target VCF (or all, if not present) int ntmpi2 = bcf_get_info_float(args->hdr, line, col->hdr_key, &args->tmpi2, &args->mtmpi2); if ( ntmpi2 < ndst ) hts_expand(int32_t,ndst,args->mtmpi2,args->tmpi2); int i; for (i=0; itmpi2[i] = bcf_int32_missing; continue; } if ( ntmpi2==ndst && col->replace==REPLACE_MISSING && args->tmpi2[i]!=bcf_int32_missing && args->tmpi2[i]!=bcf_int32_vector_end ) continue; args->tmpi2[i] = args->tmpi[ map[i] ]; } bcf_update_info_int32(args->hdr_out,line,col->hdr_key,args->tmpi2,ndst); return 0; } static int setter_info_int(args_t *args, bcf1_t *line, annot_col_t *col, void *data) { annot_line_t *tab = (annot_line_t*) data; char *str = tab->cols[col->icol], *end = str; if ( str[0]=='.' && str[1]==0 ) return 0; int ntmpi = 0; while ( *end ) { int val = strtol(str, &end, 10); if ( end==str ) error("Could not parse %s at %s:%d .. [%s]\n", bcf_seqname(args->hdr,line),line->pos+1,tab->cols[col->icol]); ntmpi++; hts_expand(int32_t,ntmpi,args->mtmpi,args->tmpi); args->tmpi[ntmpi-1] = val; str = end+1; } if ( col->number==BCF_VL_A || col->number==BCF_VL_R ) return setter_ARinfo_int32(args,line,col,tab->nals,tab->als,ntmpi); if ( col->replace==REPLACE_MISSING ) { int ret = bcf_get_info_int32(args->hdr, line, col->hdr_key, &args->tmpi2, &args->mtmpi2); if ( ret>0 && args->tmpi2[0]!=bcf_int32_missing ) return 0; } bcf_update_info_int32(args->hdr_out,line,col->hdr_key,args->tmpi,ntmpi); return 0; } static int vcf_setter_info_int(args_t *args, bcf1_t *line, annot_col_t *col, void *data) { bcf1_t *rec = (bcf1_t*) data; int ntmpi = bcf_get_info_int32(args->files->readers[1].header,rec,col->hdr_key,&args->tmpi,&args->mtmpi); if ( ntmpi < 0 ) return 0; // nothing to add if ( col->number==BCF_VL_A || col->number==BCF_VL_R ) return setter_ARinfo_int32(args,line,col,rec->n_allele,rec->d.allele,ntmpi); if ( col->replace==REPLACE_MISSING ) { int ret = bcf_get_info_int32(args->hdr, line, col->hdr_key, &args->tmpi2, &args->mtmpi2); if ( ret>0 && args->tmpi2[0]!=bcf_int32_missing ) return 0; } bcf_update_info_int32(args->hdr_out,line,col->hdr_key,args->tmpi,ntmpi); return 0; } static int setter_ARinfo_real(args_t *args, bcf1_t *line, annot_col_t *col, int nals, char **als, int ntmpf) { if ( col->number==BCF_VL_A && ntmpf!=nals-1 && (ntmpf!=1 || !bcf_float_is_missing(args->tmpf[0]) || !bcf_float_is_vector_end(args->tmpf[0])) ) error("Incorrect number of values (%d) for the %s tag at %s:%d\n", ntmpf,col->hdr_key,bcf_seqname(args->hdr,line),line->pos+1); else if ( col->number==BCF_VL_R && ntmpf!=nals && (ntmpf!=1 || !bcf_float_is_missing(args->tmpf[0]) || !bcf_float_is_vector_end(args->tmpf[0])) ) error("Incorrect number of values (%d) for the %s tag at %s:%d\n", ntmpf,col->hdr_key,bcf_seqname(args->hdr,line),line->pos+1); int ndst = col->number==BCF_VL_A ? line->n_allele - 1 : line->n_allele; int *map = vcmp_map_ARvalues(args->vcmp,ndst,nals,als,line->n_allele,line->d.allele); if ( !map ) error("REF alleles not compatible at %s:%d\n"); // fill in any missing values in the target VCF (or all, if not present) int ntmpf2 = bcf_get_info_float(args->hdr, line, col->hdr_key, &args->tmpf2, &args->mtmpf2); if ( ntmpf2 < ndst ) hts_expand(float,ndst,args->mtmpf2,args->tmpf2); int i; for (i=0; itmpf2[i]); continue; } if ( ntmpf2==ndst && col->replace==REPLACE_MISSING && !bcf_float_is_missing(args->tmpf2[i]) && !bcf_float_is_vector_end(args->tmpf2[i]) ) continue; args->tmpf2[i] = args->tmpf[ map[i] ]; } bcf_update_info_float(args->hdr_out,line,col->hdr_key,args->tmpf2,ndst); return 0; } static int setter_info_real(args_t *args, bcf1_t *line, annot_col_t *col, void *data) { annot_line_t *tab = (annot_line_t*) data; char *str = tab->cols[col->icol], *end = str; if ( str[0]=='.' && str[1]==0 ) return 0; int ntmpf = 0; while ( *end ) { double val = strtod(str, &end); if ( end==str ) error("Could not parse %s at %s:%d .. [%s]\n", bcf_seqname(args->hdr,line),line->pos+1,tab->cols[col->icol]); ntmpf++; hts_expand(float,ntmpf,args->mtmpf,args->tmpf); args->tmpf[ntmpf-1] = val; str = end+1; } if ( col->number==BCF_VL_A || col->number==BCF_VL_R ) return setter_ARinfo_real(args,line,col,tab->nals,tab->als,ntmpf); if ( col->replace==REPLACE_MISSING ) { int ret = bcf_get_info_float(args->hdr, line, col->hdr_key, &args->tmpf2, &args->mtmpf2); if ( ret>0 && !bcf_float_is_missing(args->tmpf2[0]) ) return 0; } bcf_update_info_float(args->hdr_out,line,col->hdr_key,args->tmpf,ntmpf); return 0; } static int vcf_setter_info_real(args_t *args, bcf1_t *line, annot_col_t *col, void *data) { bcf1_t *rec = (bcf1_t*) data; int ntmpf = bcf_get_info_float(args->files->readers[1].header,rec,col->hdr_key,&args->tmpf,&args->mtmpf); if ( ntmpf < 0 ) return 0; // nothing to add if ( col->number==BCF_VL_A || col->number==BCF_VL_R ) return setter_ARinfo_real(args,line,col,rec->n_allele,rec->d.allele,ntmpf); if ( col->replace==REPLACE_MISSING ) { int ret = bcf_get_info_float(args->hdr, line, col->hdr_key, &args->tmpf2, &args->mtmpf2); if ( ret>0 && !bcf_float_is_missing(args->tmpf2[0]) ) return 0; } bcf_update_info_float(args->hdr_out,line,col->hdr_key,args->tmpf,ntmpf); return 0; } int copy_string_field(char *src, int isrc, int src_len, kstring_t *dst, int idst); // see vcfmerge.c static int setter_ARinfo_string(args_t *args, bcf1_t *line, annot_col_t *col, int nals, char **als) { int nsrc = 1, lsrc = 0; while ( args->tmps[lsrc] ) { if ( args->tmps[lsrc]==',' ) nsrc++; lsrc++; } if ( col->number==BCF_VL_A && nsrc!=nals-1 && (nsrc!=1 || args->tmps[0]!='.' || args->tmps[1]!=0 ) ) error("Incorrect number of values (%d) for the %s tag at %s:%d\n", nsrc,col->hdr_key,bcf_seqname(args->hdr,line),line->pos+1); else if ( col->number==BCF_VL_R && nsrc!=nals && (nsrc!=1 || args->tmps[0]!='.' || args->tmps[1]!=0 ) ) error("Incorrect number of values (%d) for the %s tag at %s:%d\n", nsrc,col->hdr_key,bcf_seqname(args->hdr,line),line->pos+1); int ndst = col->number==BCF_VL_A ? line->n_allele - 1 : line->n_allele; int *map = vcmp_map_ARvalues(args->vcmp,ndst,nals,als,line->n_allele,line->d.allele); if ( !map ) error("REF alleles not compatible at %s:%d\n"); // fill in any missing values in the target VCF (or all, if not present) int i, empty = 0, nstr, mstr = args->tmpks.m; nstr = bcf_get_info_string(args->hdr, line, col->hdr_key, &args->tmpks.s, &mstr); args->tmpks.m = mstr; if ( nstr<0 || (nstr==1 && args->tmpks.s[0]=='.' && args->tmpks.s[1]==0) ) { empty = 0; args->tmpks.l = 0; kputc('.',&args->tmpks); for (i=1; itmpks); } else args->tmpks.l = nstr; for (i=0; itmpks,i); continue; } if ( col->replace==REPLACE_MISSING ) { // Do not replace filled values. The field must be looked up again because // of realloc in copy_string_field int n = 0; char *str = args->tmpks.s; while ( *str && ntmps,map[i],lsrc,&args->tmpks,i); assert( ret==0 ); } bcf_update_info_string(args->hdr_out,line,col->hdr_key,args->tmpks.s); return 0; } static int setter_info_str(args_t *args, bcf1_t *line, annot_col_t *col, void *data) { annot_line_t *tab = (annot_line_t*) data; int len = strlen(tab->cols[col->icol]); if ( !len ) return 0; hts_expand(char,len+1,args->mtmps,args->tmps); memcpy(args->tmps,tab->cols[col->icol],len+1); if ( args->tmps[0]=='.' && args->tmps[1]==0 ) return 0; if ( col->number==BCF_VL_A || col->number==BCF_VL_R ) return setter_ARinfo_string(args,line,col,tab->nals,tab->als); if ( col->replace==REPLACE_MISSING ) { int ret = bcf_get_info_string(args->hdr, line, col->hdr_key, &args->tmps2, &args->mtmps2); if ( ret>0 && (args->tmps2[0]!='.' || args->tmps2[1]!=0) ) return 0; } bcf_update_info_string(args->hdr_out,line,col->hdr_key,args->tmps); return 0; } static int vcf_setter_info_str(args_t *args, bcf1_t *line, annot_col_t *col, void *data) { bcf1_t *rec = (bcf1_t*) data; int ntmps = bcf_get_info_string(args->files->readers[1].header,rec,col->hdr_key,&args->tmps,&args->mtmps); if ( ntmps < 0 ) return 0; // nothing to add if ( col->number==BCF_VL_A || col->number==BCF_VL_R ) return setter_ARinfo_string(args,line,col,rec->n_allele,rec->d.allele); if ( col->replace==REPLACE_MISSING ) { int ret = bcf_get_info_string(args->hdr, line, col->hdr_key, &args->tmps2, &args->mtmps2); if ( ret>0 && (args->tmps2[0]!='.' || args->tmps2[1]!=0) ) return 0; } bcf_update_info_string(args->hdr_out,line,col->hdr_key,args->tmps); return 0; } static int vcf_setter_format_gt(args_t *args, bcf1_t *line, annot_col_t *col, void *data) { bcf1_t *rec = (bcf1_t*) data; int nsrc = bcf_get_genotypes(args->files->readers[1].header,rec,&args->tmpi,&args->mtmpi); if ( nsrc==-3 ) return 0; // the tag is not present if ( nsrc<=0 ) return 1; // error if ( !args->sample_map ) return bcf_update_genotypes(args->hdr_out,line,args->tmpi,nsrc); int i, j, ndst = bcf_get_genotypes(args->hdr,line,&args->tmpi2,&args->mtmpi2); if ( ndst > 0 ) ndst /= bcf_hdr_nsamples(args->hdr_out); nsrc /= bcf_hdr_nsamples(args->files->readers[1].header); if ( ndst<=0 ) // field not present in dst file { if ( col->replace==REPLACE_EXISTING ) return 0; hts_expand(int32_t, nsrc*bcf_hdr_nsamples(args->hdr_out), args->mtmpi2, args->tmpi2); for (i=0; ihdr_out); i++) { int32_t *dst = args->tmpi2 + nsrc*i; if ( args->sample_map[i]==-1 ) { dst[0] = bcf_gt_missing; for (j=1; jtmpi + nsrc*args->sample_map[i]; for (j=0; jhdr_out,line,args->tmpi2,nsrc*bcf_hdr_nsamples(args->hdr_out)); } else if ( ndst >= nsrc ) { for (i=0; ihdr_out); i++) { if ( args->sample_map[i]==-1 ) continue; int32_t *src = args->tmpi + nsrc*args->sample_map[i]; int32_t *dst = args->tmpi2 + ndst*i; if ( col->replace==REPLACE_EXISTING && bcf_gt_is_missing(dst[0]) ) continue; if ( col->replace==REPLACE_MISSING && !bcf_gt_is_missing(dst[0]) ) continue; for (j=0; jhdr_out,line,args->tmpi2,ndst*bcf_hdr_nsamples(args->hdr_out)); } else // ndst < nsrc { hts_expand(int32_t, nsrc*bcf_hdr_nsamples(args->hdr_out), args->mtmpi3, args->tmpi3); for (i=0; ihdr_out); i++) { int32_t *ori = args->tmpi2 + ndst*i; int32_t *dst = args->tmpi3 + nsrc*i; int keep_ori = 0; if ( args->sample_map[i]==-1 ) keep_ori = 1; else if ( col->replace==REPLACE_EXISTING && bcf_gt_is_missing(ori[0]) ) keep_ori = 1; else if ( col->replace==REPLACE_MISSING && !bcf_gt_is_missing(ori[0]) ) keep_ori = 1; if ( keep_ori ) { for (j=0; jtmpi + nsrc*args->sample_map[i]; for (j=0; jhdr_out,line,args->tmpi3,nsrc*bcf_hdr_nsamples(args->hdr_out)); } } static int vcf_setter_format_int(args_t *args, bcf1_t *line, annot_col_t *col, void *data) { bcf1_t *rec = (bcf1_t*) data; int nsrc = bcf_get_format_int32(args->files->readers[1].header,rec,col->hdr_key,&args->tmpi,&args->mtmpi); if ( nsrc==-3 ) return 0; // the tag is not present if ( nsrc<=0 ) return 1; // error if ( !args->sample_map ) return bcf_update_format_int32(args->hdr_out,line,col->hdr_key,args->tmpi,nsrc); int i, j, ndst = bcf_get_format_int32(args->hdr,line,col->hdr_key,&args->tmpi2,&args->mtmpi2); if ( ndst > 0 ) ndst /= bcf_hdr_nsamples(args->hdr_out); nsrc /= bcf_hdr_nsamples(args->files->readers[1].header); if ( ndst<=0 ) { if ( col->replace==REPLACE_EXISTING ) return 0; // overwrite only if present hts_expand(int32_t, nsrc*bcf_hdr_nsamples(args->hdr_out), args->mtmpi2, args->tmpi2); for (i=0; ihdr_out); i++) { int32_t *dst = args->tmpi2 + nsrc*i; if ( args->sample_map[i]==-1 ) { dst[0] = bcf_int32_missing; for (j=1; jtmpi + nsrc*args->sample_map[i]; for (j=0; jhdr_out,line,col->hdr_key,args->tmpi2,nsrc*bcf_hdr_nsamples(args->hdr_out)); } else if ( ndst >= nsrc ) { for (i=0; ihdr_out); i++) { if ( args->sample_map[i]==-1 ) continue; int32_t *src = args->tmpi + nsrc*args->sample_map[i]; int32_t *dst = args->tmpi2 + ndst*i; if ( col->replace==REPLACE_EXISTING && dst[0]==bcf_int32_missing ) continue; if ( col->replace==REPLACE_MISSING && dst[0]!=bcf_int32_missing ) continue; for (j=0; jhdr_out,line,col->hdr_key,args->tmpi2,ndst*bcf_hdr_nsamples(args->hdr_out)); } else // ndst < nsrc { hts_expand(int32_t, nsrc*bcf_hdr_nsamples(args->hdr_out), args->mtmpi3, args->tmpi3); for (i=0; ihdr_out); i++) { int32_t *ori = args->tmpi2 + ndst*i; int32_t *dst = args->tmpi3 + nsrc*i; int keep_ori = 0; if ( args->sample_map[i]==-1 ) keep_ori = 1; else if ( col->replace==REPLACE_EXISTING && ori[0]==bcf_int32_missing ) keep_ori = 1; else if ( col->replace==REPLACE_MISSING && ori[0]!=bcf_int32_missing ) keep_ori = 1; if ( keep_ori ) { for (j=0; jtmpi + nsrc*args->sample_map[i]; for (j=0; jhdr_out,line,col->hdr_key,args->tmpi3,nsrc*bcf_hdr_nsamples(args->hdr_out)); } } static int vcf_setter_format_real(args_t *args, bcf1_t *line, annot_col_t *col, void *data) { bcf1_t *rec = (bcf1_t*) data; int nsrc = bcf_get_format_float(args->files->readers[1].header,rec,col->hdr_key,&args->tmpf,&args->mtmpf); if ( nsrc==-3 ) return 0; // the tag is not present if ( nsrc<=0 ) return 1; // error if ( !args->sample_map ) return bcf_update_format_float(args->hdr_out,line,col->hdr_key,args->tmpf,nsrc); int i, j, ndst = bcf_get_format_float(args->hdr,line,col->hdr_key,&args->tmpf2,&args->mtmpf2); if ( ndst > 0 ) ndst /= bcf_hdr_nsamples(args->hdr_out); nsrc /= bcf_hdr_nsamples(args->files->readers[1].header); if ( ndst<=0 ) { if ( col->replace==REPLACE_EXISTING ) return 0; // overwrite only if present hts_expand(float, nsrc*bcf_hdr_nsamples(args->hdr_out), args->mtmpf2, args->tmpf2); for (i=0; ihdr_out); i++) { float *dst = args->tmpf2 + nsrc*i; if ( args->sample_map[i]==-1 ) { bcf_float_set_missing(dst[0]); for (j=1; jtmpf + nsrc*args->sample_map[i]; for (j=0; jhdr_out,line,col->hdr_key,args->tmpf2,nsrc*bcf_hdr_nsamples(args->hdr_out)); } else if ( ndst >= nsrc ) { for (i=0; ihdr_out); i++) { if ( args->sample_map[i]==-1 ) continue; float *src = args->tmpf + nsrc*args->sample_map[i]; float *dst = args->tmpf2 + ndst*i; if ( col->replace==REPLACE_EXISTING && bcf_float_is_missing(dst[0]) ) continue; if ( col->replace==REPLACE_MISSING && !bcf_float_is_missing(dst[0]) ) continue; for (j=0; jhdr_out,line,col->hdr_key,args->tmpf2,ndst*bcf_hdr_nsamples(args->hdr_out)); } else // ndst < nsrc { hts_expand(float, nsrc*bcf_hdr_nsamples(args->hdr_out), args->mtmpf3, args->tmpf3); for (i=0; ihdr_out); i++) { float *ori = args->tmpf2 + ndst*i; float *dst = args->tmpf3 + nsrc*i; int keep_ori = 0; if ( args->sample_map[i]==-1 ) keep_ori = 1; else if ( col->replace==REPLACE_EXISTING && bcf_float_is_missing(ori[0]) ) keep_ori = 1; else if ( col->replace==REPLACE_MISSING && !bcf_float_is_missing(ori[0]) ) keep_ori = 1; if ( keep_ori ) { for (j=0; jtmpf + nsrc*args->sample_map[i]; for (j=0; jhdr_out,line,col->hdr_key,args->tmpf3,nsrc*bcf_hdr_nsamples(args->hdr_out)); } } static int vcf_setter_format_str(args_t *args, bcf1_t *line, annot_col_t *col, void *data) { bcf1_t *rec = (bcf1_t*) data; args->tmpp[0] = args->tmps; int ret = bcf_get_format_string(args->files->readers[1].header,rec,col->hdr_key,&args->tmpp,&args->mtmps); args->tmps = args->tmpp[0]; // tmps might be realloced if ( ret==-3 ) return 0; // the tag is not present if ( ret<=0 ) return 1; // error if ( !args->sample_map ) return bcf_update_format_string(args->hdr_out,line,col->hdr_key,(const char**)args->tmpp,bcf_hdr_nsamples(args->hdr_out)); int i; args->tmpp2[0] = args->tmps2; ret = bcf_get_format_string(args->hdr,line,col->hdr_key,&args->tmpp2,&args->mtmps2); args->tmps2 = args->tmpp2[0]; // tmps2 might be realloced if ( ret<=0 ) // not present in dst { hts_expand(char,bcf_hdr_nsamples(args->hdr_out)*2,args->mtmps2,args->tmps2); for (i=0; ihdr_out); i++) { args->tmps2[2*i] = '.'; args->tmps2[2*i+1] = 0; args->tmpp2[i] = args->tmps2+2*i; } } for (i=0; ihdr_out); i++) { int isrc = args->sample_map[i]; if ( isrc==-1 ) continue; args->tmpp2[i] = args->tmpp[isrc]; } return bcf_update_format_string(args->hdr_out,line,col->hdr_key,(const char**)args->tmpp2,bcf_hdr_nsamples(args->hdr_out)); } static void set_samples(args_t *args, bcf_hdr_t *src, bcf_hdr_t *dst, int need_samples) { int i; if ( !args->sample_names ) { int nmatch = 0, order_ok = 1; for (i=0; isamples[i]); if ( id!=-1 ) { nmatch++; if ( i!=id ) order_ok = 0; } } if ( bcf_hdr_nsamples(src)==bcf_hdr_nsamples(dst) && nmatch==bcf_hdr_nsamples(src) && order_ok && !need_samples ) return; // the same samples in both files if ( !nmatch ) error("No matching samples found in the source and the destination file\n"); if ( nmatch!=bcf_hdr_nsamples(src) || nmatch!=bcf_hdr_nsamples(dst) ) fprintf(stderr,"%d sample(s) in common\n", nmatch); args->nsample_map = bcf_hdr_nsamples(dst); args->sample_map = (int*) malloc(sizeof(int)*args->nsample_map); for (i=0; insample_map; i++) { int id = bcf_hdr_id2int(src, BCF_DT_SAMPLE, dst->samples[i]); args->sample_map[i] = id; // idst -> isrc, -1 if not present } return; } args->nsample_map = bcf_hdr_nsamples(dst); args->sample_map = (int*) malloc(sizeof(int)*args->nsample_map); for (i=0; insample_map; i++) args->sample_map[i] = -1; int nsamples = 0; char **samples = hts_readlist(args->sample_names, args->sample_is_file, &nsamples); for (i=0; isample_map[idst] = isrc; continue; } *se = 0; isrc = bcf_hdr_id2int(src, BCF_DT_SAMPLE,ss); if ( isrc==-1 ) error("Sample \"%s\" not found in the source file\n", ss); ss = se+1; while ( isspace(*ss) ) ss++; se = ss; while ( *se && !isspace(*se) ) se++; idst = bcf_hdr_id2int(dst, BCF_DT_SAMPLE,ss); if ( idst==-1 ) error("Sample \"%s\" not found in the destination file\n", ss); args->sample_map[idst] = isrc; } for (i=0; itgts_is_vcf ) args->columns = columns_complement(args->columns, &skip_info, &skip_fmt); kstring_t str = {0,0,0}, tmp = {0,0,0}; char *ss = args->columns, *se = ss; args->ncols = 0; int i = -1, has_fmt_str = 0, force_samples = -1; while ( *ss ) { if ( *se && *se!=',' ) { se++; continue; } int replace = REPLACE_ALL; if ( *ss=='+' ) { replace = REPLACE_MISSING; ss++; } else if ( *ss=='-' ) { replace = REPLACE_EXISTING; ss++; } i++; str.l = 0; kputsn(ss, se-ss, &str); if ( !str.s[0] || !strcasecmp("-",str.s) ) ; else if ( !strcasecmp("CHROM",str.s) ) args->chr_idx = i; else if ( !strcasecmp("POS",str.s) ) args->from_idx = i; else if ( !strcasecmp("FROM",str.s) ) args->from_idx = i; else if ( !strcasecmp("TO",str.s) ) args->to_idx = i; else if ( !strcasecmp("REF",str.s) ) args->ref_idx = i; else if ( !strcasecmp("ALT",str.s) ) args->alt_idx = i; else if ( !strcasecmp("ID",str.s) ) { if ( replace==REPLACE_EXISTING ) error("todo: -ID\n"); args->ncols++; args->cols = (annot_col_t*) realloc(args->cols,sizeof(annot_col_t)*args->ncols); annot_col_t *col = &args->cols[args->ncols-1]; col->icol = i; col->replace = replace; col->setter = args->tgts_is_vcf ? vcf_setter_id : setter_id; col->hdr_key = strdup(str.s); } else if ( !strcasecmp("FILTER",str.s) ) { if ( replace==REPLACE_EXISTING ) error("todo: -FILTER\n"); args->ncols++; args->cols = (annot_col_t*) realloc(args->cols,sizeof(annot_col_t)*args->ncols); annot_col_t *col = &args->cols[args->ncols-1]; col->icol = i; col->replace = replace; col->setter = args->tgts_is_vcf ? vcf_setter_filter : setter_filter; col->hdr_key = strdup(str.s); if ( args->tgts_is_vcf ) { bcf_hdr_t *tgts_hdr = args->files->readers[1].header; int j; for (j=0; jnhrec; j++) { bcf_hrec_t *hrec = tgts_hdr->hrec[j]; if ( hrec->type!=BCF_HL_FLT ) continue; int k = bcf_hrec_find_key(hrec,"ID"); assert( k>=0 ); // this should always be true for valid VCFs tmp.l = 0; bcf_hrec_format(hrec, &tmp); bcf_hdr_append(args->hdr_out, tmp.s); } bcf_hdr_sync(args->hdr_out); } } else if ( !strcasecmp("QUAL",str.s) ) { if ( replace==REPLACE_EXISTING ) error("todo: -QUAL\n"); args->ncols++; args->cols = (annot_col_t*) realloc(args->cols,sizeof(annot_col_t)*args->ncols); annot_col_t *col = &args->cols[args->ncols-1]; col->icol = i; col->replace = replace; col->setter = args->tgts_is_vcf ? vcf_setter_qual : setter_qual; col->hdr_key = strdup(str.s); } else if ( args->tgts_is_vcf && !strcasecmp("INFO",str.s) ) // All INFO fields { if ( replace==REPLACE_EXISTING ) error("todo: -INFO/TAG\n"); bcf_hdr_t *tgts_hdr = args->files->readers[1].header; int j; for (j=0; jnhrec; j++) { bcf_hrec_t *hrec = tgts_hdr->hrec[j]; if ( hrec->type!=BCF_HL_INFO ) continue; int k = bcf_hrec_find_key(hrec,"ID"); assert( k>=0 ); // this should always be true for valid VCFs if ( skip_info && khash_str2int_has_key(skip_info,hrec->vals[k]) ) continue; tmp.l = 0; bcf_hrec_format(hrec, &tmp); bcf_hdr_append(args->hdr_out, tmp.s); bcf_hdr_sync(args->hdr_out); int hdr_id = bcf_hdr_id2int(args->hdr_out, BCF_DT_ID, hrec->vals[k]); args->ncols++; args->cols = (annot_col_t*) realloc(args->cols,sizeof(annot_col_t)*args->ncols); annot_col_t *col = &args->cols[args->ncols-1]; col->icol = -1; col->replace = replace; col->hdr_key = strdup(hrec->vals[k]); col->number = bcf_hdr_id2length(args->hdr_out,BCF_HL_INFO,hdr_id); switch ( bcf_hdr_id2type(args->hdr_out,BCF_HL_INFO,hdr_id) ) { case BCF_HT_FLAG: col->setter = vcf_setter_info_flag; break; case BCF_HT_INT: col->setter = vcf_setter_info_int; break; case BCF_HT_REAL: col->setter = vcf_setter_info_real; break; case BCF_HT_STR: col->setter = vcf_setter_info_str; break; default: error("The type of %s not recognised (%d)\n", str.s,bcf_hdr_id2type(args->hdr_out,BCF_HL_INFO,hdr_id)); } } } else if ( args->tgts_is_vcf && (!strcasecmp("FORMAT",str.s) || !strcasecmp("FMT",str.s)) ) // All FORMAT fields { bcf_hdr_t *tgts_hdr = args->files->readers[1].header; if ( force_samples<0 ) force_samples = replace; if ( force_samples>=0 && replace!=REPLACE_ALL ) force_samples = replace; int j; for (j=0; jnhrec; j++) { bcf_hrec_t *hrec = tgts_hdr->hrec[j]; if ( hrec->type!=BCF_HL_FMT) continue; int k = bcf_hrec_find_key(hrec,"ID"); assert( k>=0 ); // this should always be true for valid VCFs if ( skip_fmt && khash_str2int_has_key(skip_fmt,hrec->vals[k]) ) continue; tmp.l = 0; bcf_hrec_format(hrec, &tmp); bcf_hdr_append(args->hdr_out, tmp.s); bcf_hdr_sync(args->hdr_out); int hdr_id = bcf_hdr_id2int(args->hdr_out, BCF_DT_ID, hrec->vals[k]); args->ncols++; args->cols = (annot_col_t*) realloc(args->cols,sizeof(annot_col_t)*args->ncols); annot_col_t *col = &args->cols[args->ncols-1]; col->icol = -1; col->replace = replace; col->hdr_key = strdup(hrec->vals[k]); if ( !strcasecmp("GT",col->hdr_key) ) col->setter = vcf_setter_format_gt; else switch ( bcf_hdr_id2type(args->hdr_out,BCF_HL_FMT,hdr_id) ) { case BCF_HT_INT: col->setter = vcf_setter_format_int; break; case BCF_HT_REAL: col->setter = vcf_setter_format_real; break; case BCF_HT_STR: col->setter = vcf_setter_format_str; has_fmt_str = 1; break; default: error("The type of %s not recognised (%d)\n", str.s,bcf_hdr_id2type(args->hdr_out,BCF_HL_FMT,hdr_id)); } } } else if ( args->tgts_is_vcf && (!strncasecmp("FORMAT/",str.s, 7) || !strncasecmp("FMT/",str.s,4)) ) { char *key = str.s + (!strncasecmp("FMT/",str.s,4) ? 4 : 7); if ( force_samples<0 ) force_samples = replace; if ( force_samples>=0 && replace!=REPLACE_ALL ) force_samples = replace;; bcf_hrec_t *hrec = bcf_hdr_get_hrec(args->files->readers[1].header, BCF_HL_FMT, "ID", key, NULL); tmp.l = 0; bcf_hrec_format(hrec, &tmp); bcf_hdr_append(args->hdr_out, tmp.s); bcf_hdr_sync(args->hdr_out); int hdr_id = bcf_hdr_id2int(args->hdr_out, BCF_DT_ID, key); args->ncols++; args->cols = (annot_col_t*) realloc(args->cols,sizeof(annot_col_t)*args->ncols); annot_col_t *col = &args->cols[args->ncols-1]; col->icol = -1; col->replace = replace; col->hdr_key = strdup(key); if ( !strcasecmp("GT",key) ) col->setter = vcf_setter_format_gt; else switch ( bcf_hdr_id2type(args->hdr_out,BCF_HL_FMT,hdr_id) ) { case BCF_HT_INT: col->setter = vcf_setter_format_int; break; case BCF_HT_REAL: col->setter = vcf_setter_format_real; break; case BCF_HT_STR: col->setter = vcf_setter_format_str; has_fmt_str = 1; break; default: error("The type of %s not recognised (%d)\n", str.s,bcf_hdr_id2type(args->hdr_out,BCF_HL_FMT,hdr_id)); } } else { if ( replace==REPLACE_EXISTING ) error("todo: -INFO/TAG\n"); if ( !strncasecmp("INFO/",str.s,5) ) { memmove(str.s,str.s+5,str.l-4); } int hdr_id = bcf_hdr_id2int(args->hdr_out, BCF_DT_ID, str.s); if ( !bcf_hdr_idinfo_exists(args->hdr_out,BCF_HL_INFO,hdr_id) ) { if ( args->tgts_is_vcf ) // reading annotations from a VCF, add a new header line { bcf_hrec_t *hrec = bcf_hdr_get_hrec(args->files->readers[1].header, BCF_HL_INFO, "ID", str.s, NULL); if ( !hrec ) error("The tag \"%s\" is not defined in %s\n", str.s,args->files->readers[1].fname); tmp.l = 0; bcf_hrec_format(hrec, &tmp); bcf_hdr_append(args->hdr_out, tmp.s); bcf_hdr_sync(args->hdr_out); hdr_id = bcf_hdr_id2int(args->hdr_out, BCF_DT_ID, str.s); } else error("The tag \"%s\" is not defined in %s\n", str.s, args->targets_fname); assert( bcf_hdr_idinfo_exists(args->hdr_out,BCF_HL_INFO,hdr_id) ); } args->ncols++; args->cols = (annot_col_t*) realloc(args->cols,sizeof(annot_col_t)*args->ncols); annot_col_t *col = &args->cols[args->ncols-1]; col->icol = i; col->replace = replace; col->hdr_key = strdup(str.s); col->number = bcf_hdr_id2length(args->hdr_out,BCF_HL_INFO,hdr_id); switch ( bcf_hdr_id2type(args->hdr_out,BCF_HL_INFO,hdr_id) ) { case BCF_HT_FLAG: col->setter = args->tgts_is_vcf ? vcf_setter_info_flag : setter_info_flag; break; case BCF_HT_INT: col->setter = args->tgts_is_vcf ? vcf_setter_info_int : setter_info_int; break; case BCF_HT_REAL: col->setter = args->tgts_is_vcf ? vcf_setter_info_real : setter_info_real; break; case BCF_HT_STR: col->setter = args->tgts_is_vcf ? vcf_setter_info_str : setter_info_str; break; default: error("The type of %s not recognised (%d)\n", str.s,bcf_hdr_id2type(args->hdr_out,BCF_HL_INFO,hdr_id)); } } if ( !*se ) break; ss = ++se; } free(str.s); free(tmp.s); if ( args->to_idx==-1 ) args->to_idx = args->from_idx; free(args->columns); if ( skip_info ) khash_str2int_destroy_free(skip_info); if ( skip_fmt ) khash_str2int_destroy_free(skip_fmt); if ( has_fmt_str ) { int n = bcf_hdr_nsamples(args->hdr_out) > bcf_hdr_nsamples(args->files->readers[1].header) ? bcf_hdr_nsamples(args->hdr_out) : bcf_hdr_nsamples(args->files->readers[1].header); args->tmpp = (char**)malloc(sizeof(char*)*n); args->tmpp2 = (char**)malloc(sizeof(char*)*n); } if ( force_samples>=0 ) set_samples(args, args->files->readers[1].header, args->hdr, force_samples==REPLACE_ALL ? 0 : 1); } static void rename_chrs(args_t *args, char *fname) { int n, i; char **map = hts_readlist(fname, 1, &n); if ( !map ) error("Could not read: %s\n", fname); for (i=0; ihdr_out, map[i]); bcf_hrec_t *hrec = bcf_hdr_get_hrec(args->hdr_out, BCF_HL_CTG, "ID", map[i], NULL); if ( !hrec ) continue; // the sequence not present int j = bcf_hrec_find_key(hrec, "ID"); assert( j>=0 ); free(hrec->vals[j]); ss++; while ( *ss && isspace(*ss) ) ss++; char *se = ss; while ( *se && !isspace(*se) ) se++; *se = 0; hrec->vals[j] = strdup(ss); args->hdr_out->id[BCF_DT_CTG][rid].key = hrec->vals[j]; } for (i=0; ihdr = args->files->readers[0].header; args->hdr_out = bcf_hdr_dup(args->hdr); if ( args->remove_annots ) init_remove_annots(args); if ( args->header_fname ) init_header_lines(args); if ( args->targets_fname && args->tgts_is_vcf ) { // reading annots from a VCF if ( !bcf_sr_add_reader(args->files, args->targets_fname) ) error("Failed to open %s: %s\n", args->targets_fname,bcf_sr_strerror(args->files->errnum)); } if ( args->columns ) init_columns(args); if ( args->targets_fname && !args->tgts_is_vcf ) { if ( !args->columns ) error("The -c option not given\n"); if ( args->chr_idx==-1 ) error("The -c CHROM option not given\n"); if ( args->from_idx==-1 ) error("The -c POS option not given\n"); if ( args->to_idx==-1 ) args->to_idx = -args->from_idx - 1; args->tgts = bcf_sr_regions_init(args->targets_fname,1,args->chr_idx,args->from_idx,args->to_idx); if ( !args->tgts ) error("Could not initialize the annotation file: %s\n", args->targets_fname); if ( !args->tgts->tbx ) error("Expected tabix-indexed annotation file: %s\n", args->targets_fname); } args->vcmp = vcmp_init(); if ( args->filter_str ) args->filter = filter_init(args->hdr, args->filter_str); if ( args->set_ids_fmt ) { if ( args->set_ids_fmt[0]=='+' ) { args->set_ids_replace = 0; args->set_ids_fmt++; } args->set_ids = convert_init(args->hdr_out, NULL, 0, args->set_ids_fmt); } bcf_hdr_append_version(args->hdr_out, args->argc, args->argv, "bcftools_annotate"); if ( !args->drop_header ) { if ( args->rename_chrs ) rename_chrs(args, args->rename_chrs); args->out_fh = hts_open(args->output_fname,hts_bcf_wmode(args->output_type)); if ( args->out_fh == NULL ) error("Can't write to \"%s\": %s\n", args->output_fname, strerror(errno)); bcf_hdr_write(args->out_fh, args->hdr_out); } } static void destroy_data(args_t *args) { int i; for (i=0; inrm; i++) free(args->rm[i].key); free(args->rm); if ( args->hdr_out ) bcf_hdr_destroy(args->hdr_out); if (args->vcmp) vcmp_destroy(args->vcmp); for (i=0; incols; i++) free(args->cols[i].hdr_key); free(args->cols); for (i=0; imalines; i++) { free(args->alines[i].cols); free(args->alines[i].als); free(args->alines[i].line.s); } free(args->alines); if ( args->tgts ) bcf_sr_regions_destroy(args->tgts); free(args->tmpks.s); free(args->tmpi); free(args->tmpf); free(args->tmps); free(args->tmpp); free(args->tmpi2); free(args->tmpf2); free(args->tmps2); free(args->tmpp2); free(args->tmpi3); free(args->tmpf3); if ( args->set_ids ) convert_destroy(args->set_ids); if ( args->filter ) filter_destroy(args->filter); if (args->out_fh) hts_close(args->out_fh); free(args->sample_map); } static void buffer_annot_lines(args_t *args, bcf1_t *line, int start_pos, int end_pos) { if ( args->nalines && args->alines[0].rid != line->rid ) args->nalines = 0; int i = 0; while ( inalines ) { if ( line->pos > args->alines[i].end ) { args->nalines--; if ( args->nalines && inalines ) { annot_line_t tmp = args->alines[i]; memmove(&args->alines[i],&args->alines[i+1],(args->nalines-i)*sizeof(annot_line_t)); args->alines[args->nalines] = tmp; } } else i++; } if ( args->ref_idx==-1 && args->nalines ) return; while ( !bcf_sr_regions_overlap(args->tgts, bcf_seqname(args->hdr,line), start_pos,end_pos) ) { args->nalines++; hts_expand0(annot_line_t,args->nalines,args->malines,args->alines); annot_line_t *tmp = &args->alines[args->nalines-1]; tmp->rid = line->rid; tmp->start = args->tgts->start; tmp->end = args->tgts->end; tmp->line.l = 0; kputs(args->tgts->line.s, &tmp->line); char *s = tmp->line.s; tmp->ncols = 1; hts_expand(char*,tmp->ncols,tmp->mcols,tmp->cols); tmp->cols[0] = s; while ( *s ) { if ( *s=='\t' ) { tmp->ncols++; hts_expand(char*,tmp->ncols,tmp->mcols,tmp->cols); tmp->cols[tmp->ncols-1] = s+1; *s = 0; } s++; } if ( args->ref_idx != -1 ) { assert( args->ref_idx < tmp->ncols ); assert( args->alt_idx < tmp->ncols ); tmp->nals = 2; hts_expand(char*,tmp->nals,tmp->mals,tmp->als); tmp->als[0] = tmp->cols[args->ref_idx]; tmp->als[1] = s = tmp->cols[args->alt_idx]; while ( *s ) { if ( *s==',' ) { tmp->nals++; hts_expand(char*,tmp->nals,tmp->mals,tmp->als); tmp->als[tmp->nals-1] = s+1; *s = 0; } s++; } int iseq = args->tgts->iseq; if ( bcf_sr_regions_next(args->tgts)<0 || args->tgts->iseq!=iseq ) break; } else break; } } static void annotate(args_t *args, bcf1_t *line) { int i, j; for (i=0; inrm; i++) args->rm[i].handler(args, line, &args->rm[i]); if ( args->tgts ) { // Buffer annotation lines. When multiple ALT alleles are present in the // annotation file, at least one must match one of the VCF alleles. int len = 0; bcf_get_variant_types(line); for (i=1; in_allele; i++) if ( len > line->d.var[i].n ) len = line->d.var[i].n; int end_pos = len<0 ? line->pos - len : line->pos; buffer_annot_lines(args, line, line->pos, end_pos); for (i=0; inalines; i++) { if ( line->pos > args->alines[i].end || end_pos < args->alines[i].start ) continue; if ( args->ref_idx != -1 ) { if ( vcmp_set_ref(args->vcmp, line->d.allele[0], args->alines[i].als[0]) < 0 ) continue; // refs not compatible for (j=1; jalines[i].nals; j++) { if ( line->n_allele==1 && args->alines[i].als[j][0]=='.' && args->alines[i].als[j][1]==0 ) break; // no ALT allele in VCF and annot file has "." if ( vcmp_find_allele(args->vcmp, line->d.allele+1, line->n_allele - 1, args->alines[i].als[j]) >= 0 ) break; } if ( j==args->alines[i].nals ) continue; // none of the annot alleles present in VCF's ALT } break; } if ( inalines ) { for (j=0; jncols; j++) if ( args->cols[j].setter(args,line,&args->cols[j],&args->alines[i]) ) error("fixme: Could not set %s at %s:%d\n", args->cols[j].hdr_key,bcf_seqname(args->hdr,line),line->pos+1); } } else if ( args->files->nreaders == 2 && bcf_sr_has_line(args->files,1) ) { bcf1_t *aline = bcf_sr_get_line(args->files,1); for (j=0; jncols; j++) if ( args->cols[j].setter(args,line,&args->cols[j],aline) ) error("fixme: Could not set %s at %s:%d\n", args->cols[j].hdr_key,bcf_seqname(args->hdr,line),line->pos+1); } if ( args->set_ids ) { args->tmpks.l = 0; convert_line(args->set_ids, line, &args->tmpks); if ( args->tmpks.l ) { int replace = 0; if ( args->set_ids_replace ) replace = 1; else if ( !line->d.id || (line->d.id[0]=='.' && !line->d.id[1]) ) replace = 1; if ( replace ) bcf_update_id(args->hdr_out,line,args->tmpks.s); } } } static void usage(args_t *args) { fprintf(stderr, "\n"); fprintf(stderr, "About: Annotate and edit VCF/BCF files.\n"); fprintf(stderr, "Usage: bcftools annotate [options] \n"); fprintf(stderr, "\n"); fprintf(stderr, "Options:\n"); fprintf(stderr, " -a, --annotations VCF file or tabix-indexed file with annotations: CHR\\tPOS[\\tVALUE]+\n"); fprintf(stderr, " -c, --columns list of columns in the annotation file, e.g. CHROM,POS,REF,ALT,-,INFO/TAG. See man page for details\n"); fprintf(stderr, " -e, --exclude exclude sites for which the expression is true (see man page for details)\n"); fprintf(stderr, " -h, --header-lines lines which should be appended to the VCF header\n"); fprintf(stderr, " -I, --set-id [+] set ID column, see man pagee for details\n"); fprintf(stderr, " -i, --include select sites for which the expression is true (see man pagee for details)\n"); fprintf(stderr, " -o, --output write output to a file [standard output]\n"); fprintf(stderr, " -O, --output-type b: compressed BCF, u: uncompressed BCF, z: compressed VCF, v: uncompressed VCF [v]\n"); fprintf(stderr, " -r, --regions restrict to comma-separated list of regions\n"); fprintf(stderr, " -R, --regions-file restrict to regions listed in a file\n"); fprintf(stderr, " --rename-chrs rename sequences according to map file: from\\tto\n"); fprintf(stderr, " -s, --samples [^] comma separated list of samples to annotate (or exclude with \"^\" prefix)\n"); fprintf(stderr, " -S, --samples-file [^] file of samples to annotate (or exclude with \"^\" prefix)\n"); fprintf(stderr, " -x, --remove list of annotations to remove (e.g. ID,INFO/DP,FORMAT/DP,FILTER). See man page for details\n"); fprintf(stderr, "\n"); exit(1); } int main_vcfannotate(int argc, char *argv[]) { int c; args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->argc = argc; args->argv = argv; args->files = bcf_sr_init(); args->output_fname = "-"; args->output_type = FT_VCF; args->ref_idx = args->alt_idx = args->chr_idx = args->from_idx = args->to_idx = -1; args->set_ids_replace = 1; int regions_is_file = 0; static struct option loptions[] = { {"set-id",1,0,'I'}, {"output",1,0,'o'}, {"output-type",1,0,'O'}, {"annotations",1,0,'a'}, {"include",1,0,'i'}, {"exclude",1,0,'e'}, {"regions",1,0,'r'}, {"regions-file",1,0,'R'}, {"remove",1,0,'x'}, {"columns",1,0,'c'}, {"rename-chrs",1,0,1}, {"header-lines",1,0,'h'}, {"samples",1,0,'s'}, {"samples-file",1,0,'S'}, {0,0,0,0} }; while ((c = getopt_long(argc, argv, "h:?o:O:r:R:a:x:c:i:e:S:s:I:",loptions,NULL)) >= 0) { switch (c) { case 'I': args->set_ids_fmt = optarg; break; case 's': args->sample_names = optarg; break; case 'S': args->sample_names = optarg; args->sample_is_file = 1; break; case 'c': args->columns = strdup(optarg); break; case 'o': args->output_fname = optarg; break; case 'O': switch (optarg[0]) { case 'b': args->output_type = FT_BCF_GZ; break; case 'u': args->output_type = FT_BCF; break; case 'z': args->output_type = FT_VCF_GZ; break; case 'v': args->output_type = FT_VCF; break; default: error("The output type \"%s\" not recognised\n", optarg); }; break; case 'e': args->filter_str = optarg; args->filter_logic |= FLT_EXCLUDE; break; case 'i': args->filter_str = optarg; args->filter_logic |= FLT_INCLUDE; break; case 'x': args->remove_annots = optarg; break; case 'a': args->targets_fname = optarg; break; case 'r': args->regions_list = optarg; break; case 'R': args->regions_list = optarg; regions_is_file = 1; break; case 'h': args->header_fname = optarg; break; case 1 : args->rename_chrs = optarg; break; case '?': usage(args); break; default: error("Unknown argument: %s\n", optarg); } } char *fname = NULL; if ( optind>=argc ) { if ( !isatty(fileno((FILE *)stdin)) ) fname = "-"; // reading from stdin else usage(args); } else fname = argv[optind]; if ( args->regions_list ) { if ( bcf_sr_set_regions(args->files, args->regions_list, regions_is_file)<0 ) error("Failed to read the regions: %s\n", args->regions_list); } if ( args->targets_fname ) { htsFile *fp = hts_open(args->targets_fname,"r"); htsFormat type = *hts_get_format(fp); hts_close(fp); if ( type.format==vcf || type.format==bcf ) { args->tgts_is_vcf = 1; args->files->require_index = 1; args->files->collapse |= COLLAPSE_SOME; } } if ( !bcf_sr_add_reader(args->files, fname) ) error("Failed to open %s: %s\n", fname,bcf_sr_strerror(args->files->errnum)); init_data(args); while ( bcf_sr_next_line(args->files) ) { if ( !bcf_sr_has_line(args->files,0) ) continue; bcf1_t *line = bcf_sr_get_line(args->files,0); if ( line->errcode ) error("Encountered error, cannot proceed. Please check the error output above.\n"); if ( args->filter ) { int pass = filter_test(args->filter, line, NULL); if ( args->filter_logic & FLT_EXCLUDE ) pass = pass ? 0 : 1; if ( !pass ) continue; } annotate(args, line); bcf_write1(args->out_fh, args->hdr_out, line); } destroy_data(args); bcf_sr_destroy(args->files); free(args); return 0; } bcftools-1.2/vcfcall.c000066400000000000000000000672761246371514100147730ustar00rootroot00000000000000/* vcfcall.c -- SNP/indel variant calling from VCF/BCF. Copyright (C) 2013-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include #include #include #include #include "bcftools.h" #include "call.h" #include "prob1.h" void error(const char *format, ...); #ifdef _WIN32 #define srand48(x) srand(x) #define lrand48() rand() #endif #define CF_NO_GENO 1 #define CF_INS_MISSED (1<<1) #define CF_CCALL (1<<2) #define CF_GVCF (1<<3) // (1<<4) // (1<<5) #define CF_ACGT_ONLY (1<<6) #define CF_QCALL (1<<7) #define CF_ADJLD (1<<8) #define CF_NO_INDEL (1<<9) #define CF_ANNO_MAX (1<<10) #define CF_MCALL (1<<11) #define CF_PAIRCALL (1<<12) #define CF_QCNT (1<<13) #define CF_INDEL_ONLY (1<<14) typedef struct { int flag; // combination of CF_* flags above int output_type; htsFile *bcf_in, *out_fh; char *bcf_fname, *output_fname; char **samples; // for subsampling and ploidy int nsamples, *samples_map; char *regions, *targets; // regions to process int regions_is_file, targets_is_file; bcf1_t *missed_line; call_t aux; // parameters and temporary data gvcf_t gvcf; int argc; char **argv; // int flag, prior_type, n1, n_sub, *sublist, n_perm; // uint32_t *trio_aux; // char *prior_file, **subsam; // uint8_t *ploidy; // double theta, pref, indel_frac, min_smpl_frac, min_lrt; // Permutation tests // int n_perm, *seeds; // double min_perm_p; // void *bed; } args_t; static family_t *get_family(family_t *fam, int nfam, char *name) { int i; for (i=0; ifams, call->nfams, str.s); if ( !fam ) { call->nfams++; hts_expand(family_t, call->nfams, call->mfams, call->fams); fam = &call->fams[call->nfams-1]; fam->name = strdup(str.s); for (j=0; j<3; j++) fam->sample[j] = -1; } int ploidy = 2; if ( call->flag & (CALL_CHR_X|CALL_CHR_Y) ) { if ( col_ends[3][1]=='1' ) ploidy = 1; // male: one chrX and one chrY copy else ploidy = call->flag & CALL_CHR_X ? 2 : 0; // female: two chrX copies, no chrY } sam = add_sample(sam, &n, &max, col_ends[0]+1, ploidy, &j); if ( strcmp(col_ends[1]+1,"0") ) // father { if ( fam->sample[CHILD]>=0 ) error("Multiple childs in %s [%s,%s]\n", str.s, sam[j],sam[fam->sample[CHILD]]); fam->sample[CHILD] = j; if ( fam->sample[FATHER]>=0 ) error("Two fathers in %s?\n", str.s); sam = add_sample(sam, &n, &max, col_ends[1]+1, call->flag & (CALL_CHR_X|CALL_CHR_Y) ? 1 : 2, &fam->sample[FATHER]); } if ( strcmp(col_ends[2]+1,"0") ) // mother { if ( fam->sample[MOTHER]>=0 ) error("Two mothers in %s?\n", str.s); sam = add_sample(sam, &n, &max, col_ends[2]+1, call->flag & CALL_CHR_Y ? 0 : 2, &fam->sample[MOTHER]); } } free(str.s); if ( i!=_n ) // not a ped file { if ( i>0 ) error("Could not parse the samples, thought it was PED format, some rows have 5 columns?!\n"); return NULL; } assert( n==_n ); for (i=0; infams; i++) assert( call->fams[i].sample[0]>=0 && call->fams[i].sample[1]>=0 && call->fams[i].sample[2]>=0 ); // multiple childs, not a trio return sam; } /* * Reads sample names and their ploidy (optional) from a file. * Alternatively, if no such file exists, the file name is interpreted * as a comma-separated list of samples. When ploidy is not present, * the default ploidy 2 is assumed. * * Returns an array of sample names, where the byte value just after \0 * indicates the ploidy. */ static char **read_samples(call_t *call, const char *fn, int is_file, int *_n) { int i, n; char **vals = hts_readlist(fn, is_file, &n); if ( !vals ) error("Could not read the file: %s\n", fn); char **smpls = parse_ped_samples(call, vals, n); if ( !smpls ) { smpls = (char**) malloc(sizeof(char*)*n); for (i=0; iaux.hdr); i++) { args->aux.gts[i*2] = bcf_gt_missing; args->aux.gts[i*2+1] = bcf_int32_vector_end; } args->missed_line = bcf_init1(); bcf_update_genotypes(args->aux.hdr, args->missed_line, args->aux.gts, 2*bcf_hdr_nsamples(args->aux.hdr)); bcf_float_set_missing(args->missed_line->qual); } static void print_missed_line(bcf_sr_regions_t *regs, void *data) { args_t *args = (args_t*) data; call_t *call = &args->aux; bcf1_t *missed = args->missed_line; if ( args->flag & CF_GVCF ) error("todo: Combine --gvcf and --insert-missed\n"); char *ss = regs->line.s; int i = 0; while ( iaux.srs->targets_als-1 && *ss ) { if ( *ss=='\t' ) i++; ss++; } if ( !*ss ) error("Could not parse: [%s] (%d)\n", regs->line.s,args->aux.srs->targets_als); missed->rid = bcf_hdr_name2id(call->hdr,regs->seq_names[regs->prev_seq]); missed->pos = regs->start; bcf_update_alleles_str(call->hdr, missed,ss); bcf_write1(args->out_fh, call->hdr, missed); } static void init_data(args_t *args) { args->aux.srs = bcf_sr_init(); // Open files for input and output, initialize structures if ( args->targets ) { if ( bcf_sr_set_targets(args->aux.srs, args->targets, args->targets_is_file, args->aux.flag&CALL_CONSTR_ALLELES ? 3 : 0)<0 ) error("Failed to read the targets: %s\n", args->targets); if ( args->aux.flag&CALL_CONSTR_ALLELES && args->flag&CF_INS_MISSED ) { args->aux.srs->targets->missed_reg_handler = print_missed_line; args->aux.srs->targets->missed_reg_data = args; } } if ( args->regions ) { if ( bcf_sr_set_regions(args->aux.srs, args->regions, args->regions_is_file)<0 ) error("Failed to read the targets: %s\n", args->regions); } int i; if ( !bcf_sr_add_reader(args->aux.srs, args->bcf_fname) ) error("Failed to open %s: %s\n", args->bcf_fname,bcf_sr_strerror(args->aux.srs->errnum)); if ( args->nsamples && args->nsamples != bcf_hdr_nsamples(args->aux.srs->readers[0].header) ) { args->samples_map = (int *) malloc(sizeof(int)*args->nsamples); args->aux.hdr = bcf_hdr_subset(args->aux.srs->readers[0].header, args->nsamples, args->samples, args->samples_map); if ( !args->aux.hdr ) error("Error occurred while subsetting samples\n"); for (i=0; insamples; i++) if ( args->samples_map[i]<0 ) error("No such sample: %s\n", args->samples[i]); if ( !bcf_hdr_nsamples(args->aux.hdr) ) error("No matching sample found\n"); } else { args->aux.hdr = bcf_hdr_dup(args->aux.srs->readers[0].header); for (i=0; insamples; i++) if ( bcf_hdr_id2int(args->aux.hdr,BCF_DT_SAMPLE,args->samples[i])<0 ) error("No such sample: %s\n", args->samples[i]); } // Reorder ploidy and family indexes to match mpileup's output and exclude samples which are not available if ( args->aux.ploidy ) { for (i=0; iaux.nfams; i++) { int j; for (j=0; j<3; j++) { int k = bcf_hdr_id2int(args->aux.hdr, BCF_DT_SAMPLE, args->samples[ args->aux.fams[i].sample[j] ]); if ( k<0 ) error("No such sample: %s\n", args->samples[ args->aux.fams[i].sample[j] ]); args->aux.fams[i].sample[j] = k; } } uint8_t *ploidy = (uint8_t*) calloc(bcf_hdr_nsamples(args->aux.hdr), 1); for (i=0; insamples; i++) // i index in -s sample list { int j = bcf_hdr_id2int(args->aux.hdr, BCF_DT_SAMPLE, args->samples[i]); // j index in the output VCF / subset VCF if ( j<0 ) { fprintf(stderr,"Warning: no such sample: \"%s\"\n", args->samples[i]); continue; } ploidy[j] = args->aux.ploidy[i]; } args->nsamples = bcf_hdr_nsamples(args->aux.hdr); for (i=0; insamples; i++) assert( ploidy[i]==0 || ploidy[i]==1 || ploidy[i]==2 ); free(args->aux.ploidy); args->aux.ploidy = ploidy; } args->out_fh = hts_open(args->output_fname, hts_bcf_wmode(args->output_type)); if ( args->out_fh == NULL ) error("Can't write to \"%s\": %s\n", args->output_fname, strerror(errno)); if ( args->flag & CF_QCALL ) return; if ( args->flag & CF_MCALL ) mcall_init(&args->aux); if ( args->flag & CF_CCALL ) ccall_init(&args->aux); if ( args->flag&CF_GVCF ) { bcf_hdr_append(args->aux.hdr,"##INFO="); args->gvcf.rid = -1; args->gvcf.line = bcf_init1(); args->gvcf.gt = (int32_t*) malloc(2*sizeof(int32_t)*bcf_hdr_nsamples(args->aux.hdr)); for (i=0; iaux.hdr); i++) { args->gvcf.gt[2*i+0] = bcf_gt_unphased(0); args->gvcf.gt[2*i+1] = bcf_gt_unphased(0); } } bcf_hdr_remove(args->aux.hdr, BCF_HL_INFO, "QS"); bcf_hdr_remove(args->aux.hdr, BCF_HL_INFO, "I16"); bcf_hdr_append_version(args->aux.hdr, args->argc, args->argv, "bcftools_call"); bcf_hdr_write(args->out_fh, args->aux.hdr); if ( args->flag&CF_INS_MISSED ) init_missed_line(args); } static void destroy_data(args_t *args) { if ( args->flag & CF_CCALL ) ccall_destroy(&args->aux); else if ( args->flag & CF_MCALL ) mcall_destroy(&args->aux); else if ( args->flag & CF_QCALL ) qcall_destroy(&args->aux); int i; for (i=0; insamples; i++) free(args->samples[i]); if ( args->aux.fams ) { for (i=0; iaux.nfams; i++) free(args->aux.fams[i].name); free(args->aux.fams); } if ( args->missed_line ) bcf_destroy(args->missed_line); if ( args->gvcf.line ) bcf_destroy(args->gvcf.line); free(args->gvcf.gt); free(args->gvcf.dp); free(args->samples); free(args->samples_map); free(args->aux.ploidy); bcf_hdr_destroy(args->aux.hdr); hts_close(args->out_fh); bcf_sr_destroy(args->aux.srs); } void parse_novel_rate(args_t *args, const char *str) { if ( sscanf(str,"%le,%le,%le",&args->aux.trio_Pm_SNPs,&args->aux.trio_Pm_del,&args->aux.trio_Pm_ins)==3 ) // explicit for all { args->aux.trio_Pm_SNPs = 1 - args->aux.trio_Pm_SNPs; args->aux.trio_Pm_del = 1 - args->aux.trio_Pm_del; args->aux.trio_Pm_ins = 1 - args->aux.trio_Pm_ins; } else if ( sscanf(str,"%le,%le",&args->aux.trio_Pm_SNPs,&args->aux.trio_Pm_del)==2 ) // dynamic for indels { args->aux.trio_Pm_SNPs = 1 - args->aux.trio_Pm_SNPs; args->aux.trio_Pm_ins = -1; // negative value for dynamic calculation } else if ( sscanf(str,"%le",&args->aux.trio_Pm_SNPs)==1 ) // same for all { args->aux.trio_Pm_SNPs = 1 - args->aux.trio_Pm_SNPs; args->aux.trio_Pm_del = -1; args->aux.trio_Pm_ins = -1; } else error("Could not parse --novel-rate %s\n", str); } static int parse_format_flag(const char *str) { int flag = 0; const char *ss = str; while ( *ss ) { const char *se = ss; while ( *se && *se!=',' ) se++; if ( !strncasecmp(ss,"GQ",se-ss) ) flag |= CALL_FMT_GQ; else if ( !strncasecmp(ss,"GP",se-ss) ) flag |= CALL_FMT_GP; else { fprintf(stderr,"Could not parse \"%s\"\n", str); exit(1); } if ( !*se ) break; ss = se + 1; } return flag; } static void usage(args_t *args) { fprintf(stderr, "\n"); fprintf(stderr, "About: SNP/indel variant calling from VCF/BCF. To be used in conjunction with samtools mpileup.\n"); fprintf(stderr, " This command replaces the former \"bcftools view\" caller. Some of the original\n"); fprintf(stderr, " functionality has been temporarily lost in the process of transition to htslib,\n"); fprintf(stderr, " but will be added back on popular demand. The original calling model can be\n"); fprintf(stderr, " invoked with the -c option.\n"); fprintf(stderr, "Usage: bcftools call [options] \n"); fprintf(stderr, "\n"); fprintf(stderr, "File format options:\n"); fprintf(stderr, " -o, --output write output to a file [standard output]\n"); fprintf(stderr, " -O, --output-type output type: 'b' compressed BCF; 'u' uncompressed BCF; 'z' compressed VCF; 'v' uncompressed VCF [v]\n"); fprintf(stderr, " -r, --regions restrict to comma-separated list of regions\n"); fprintf(stderr, " -R, --regions-file restrict to regions listed in a file\n"); fprintf(stderr, " -s, --samples list of samples to include [all samples]\n"); fprintf(stderr, " -S, --samples-file PED file or a file with optional second column for ploidy (0, 1 or 2) [all samples]\n"); fprintf(stderr, " -t, --targets similar to -r but streams rather than index-jumps\n"); fprintf(stderr, " -T, --targets-file similar to -R but streams rather than index-jumps\n"); fprintf(stderr, "\n"); fprintf(stderr, "Input/output options:\n"); fprintf(stderr, " -A, --keep-alts keep all possible alternate alleles at variant sites\n"); fprintf(stderr, " -f, --format-fields output format fields: GQ,GP (lowercase allowed) []\n"); fprintf(stderr, " -g, --gvcf output gVCF blocks of homozygous REF calls\n"); fprintf(stderr, " -i, --insert-missed output also sites missed by mpileup but present in -T\n"); fprintf(stderr, " -M, --keep-masked-ref keep sites with masked reference allele (REF=N)\n"); fprintf(stderr, " -V, --skip-variants skip indels/snps\n"); fprintf(stderr, " -v, --variants-only output variant sites only\n"); fprintf(stderr, "\n"); fprintf(stderr, "Consensus/variant calling options:\n"); fprintf(stderr, " -c, --consensus-caller the original calling method (conflicts with -m)\n"); fprintf(stderr, " -C, --constrain one of: alleles, trio (see manual)\n"); fprintf(stderr, " -m, --multiallelic-caller alternative model for multiallelic and rare-variant calling (conflicts with -c)\n"); fprintf(stderr, " -n, --novel-rate ,[...] likelihood of novel mutation for constrained trio calling, see man page for details [1e-8,1e-9,1e-9]\n"); fprintf(stderr, " -p, --pval-threshold variant if P(ref|D) mutation rate (use bigger for greater sensitivity) [1.1e-3]\n"); fprintf(stderr, " -X, --chromosome-X haploid output for male samples (requires PED file with -s)\n"); fprintf(stderr, " -Y, --chromosome-Y haploid output for males and skips females (requires PED file with -s)\n"); // todo (and more) // fprintf(stderr, "\nContrast calling and association test options:\n"); // fprintf(stderr, " -1 INT number of group-1 samples [0]\n"); // fprintf(stderr, " -C FLOAT posterior constrast for LRTaux.min_lrt); // fprintf(stderr, " -U INT number of permutations for association testing (effective with -1) [0]\n"); // fprintf(stderr, " -X FLOAT only perform permutations for P(chi^2)aux.min_perm_p); fprintf(stderr, "\n"); exit(-1); } int main_vcfcall(int argc, char *argv[]) { char *samples_fname = NULL; args_t args; memset(&args, 0, sizeof(args_t)); args.argc = argc; args.argv = argv; args.aux.prior_type = -1; args.aux.indel_frac = -1; args.aux.theta = 1.1e-3; args.aux.pref = 0.5; args.aux.min_perm_p = 0.01; args.aux.min_lrt = 1; args.flag = CF_ACGT_ONLY; args.output_fname = "-"; args.output_type = FT_VCF; args.aux.trio_Pm_SNPs = 1 - 1e-8; args.aux.trio_Pm_ins = args.aux.trio_Pm_del = 1 - 1e-9; int i, c, samples_is_file = 0; static struct option loptions[] = { {"help",0,0,'h'}, {"gvcf",1,0,'g'}, {"format-fields",1,0,'f'}, {"output",1,0,'o'}, {"output-type",1,0,'O'}, {"regions",1,0,'r'}, {"regions-file",1,0,'R'}, {"samples",1,0,'s'}, {"samples-file",1,0,'S'}, {"targets",1,0,'t'}, {"targets-file",1,0,'T'}, {"keep-alts",0,0,'A'}, {"insert-missed",0,0,'i'}, {"skip-Ns",0,0,'N'}, // now the new default {"keep-masked-refs",0,0,'M'}, {"skip-variants",1,0,'V'}, {"variants-only",0,0,'v'}, {"consensus-caller",0,0,'c'}, {"constrain",1,0,'C'}, {"multiallelic-caller",0,0,'m'}, {"pval-threshold",1,0,'p'}, {"prior",1,0,'P'}, {"chromosome-X",0,0,'X'}, {"chromosome-Y",0,0,'Y'}, {"novel-rate",1,0,'n'}, {0,0,0,0} }; char *tmp = NULL; while ((c = getopt_long(argc, argv, "h?o:O:r:R:s:S:t:T:ANMV:vcmp:C:XYn:P:f:ig:", loptions, NULL)) >= 0) { switch (c) { case 'g': args.flag |= CF_GVCF; args.gvcf.min_dp = strtol(optarg,&tmp,10); if ( *tmp ) error("Could not parse, expected integer argument: -g %s\n", optarg); break; case 'f': args.aux.output_tags |= parse_format_flag(optarg); break; case 'M': args.flag &= ~CF_ACGT_ONLY; break; // keep sites where REF is N case 'N': args.flag |= CF_ACGT_ONLY; break; // omit sites where first base in REF is N (the new default) case 'A': args.aux.flag |= CALL_KEEPALT; break; case 'c': args.flag |= CF_CCALL; break; // the original EM based calling method case 'i': args.flag |= CF_INS_MISSED; break; case 'v': args.aux.flag |= CALL_VARONLY; break; case 'o': args.output_fname = optarg; break; case 'O': switch (optarg[0]) { case 'b': args.output_type = FT_BCF_GZ; break; case 'u': args.output_type = FT_BCF; break; case 'z': args.output_type = FT_VCF_GZ; break; case 'v': args.output_type = FT_VCF; break; default: error("The output type \"%s\" not recognised\n", optarg); } break; case 'C': if ( !strcasecmp(optarg,"alleles") ) args.aux.flag |= CALL_CONSTR_ALLELES; else if ( !strcasecmp(optarg,"trio") ) args.aux.flag |= CALL_CONSTR_TRIO; else error("Unknown argument to -C: \"%s\"\n", optarg); break; case 'X': args.aux.flag |= CALL_CHR_X; break; case 'Y': args.aux.flag |= CALL_CHR_Y; break; case 'V': if ( !strcasecmp(optarg,"snps") ) args.flag |= CF_INDEL_ONLY; else if ( !strcasecmp(optarg,"indels") ) args.flag |= CF_NO_INDEL; else error("Unknown skip category \"%s\" (-S argument must be \"snps\" or \"indels\")\n", optarg); break; case 'm': args.flag |= CF_MCALL; break; // multiallelic calling method case 'p': args.aux.pref = strtod(optarg,&tmp); if ( *tmp ) error("Could not parse: --pval-threshold %s\n", optarg); break; case 'P': args.aux.theta = strtod(optarg,&tmp); if ( *tmp ) error("Could not parse, expected float argument: -P %s\n", optarg); break; case 'n': parse_novel_rate(&args,optarg); break; case 'r': args.regions = optarg; break; case 'R': args.regions = optarg; args.regions_is_file = 1; break; case 't': args.targets = optarg; break; case 'T': args.targets = optarg; args.targets_is_file = 1; break; case 's': samples_fname = optarg; break; case 'S': samples_fname = optarg; samples_is_file = 1; break; default: usage(&args); } } if ( optind>=argc ) { if ( !isatty(fileno((FILE *)stdin)) ) args.bcf_fname = "-"; // reading from stdin else usage(&args); } else args.bcf_fname = argv[optind++]; // Sanity check options and initialize if ( samples_fname ) { args.samples = read_samples(&args.aux, samples_fname, samples_is_file, &args.nsamples); args.aux.ploidy = (uint8_t*) calloc(args.nsamples+1, 1); args.aux.all_diploid = 1; for (i=0; i 1 ) error("Only one of -c or -m options can be given\n"); if ( !(args.flag & CF_CCALL) && !(args.flag & CF_MCALL) && !(args.flag & CF_QCALL) ) error("Expected -c or -m option\n"); if ( args.aux.n_perm && args.aux.ngrp1_samples<=0 ) error("Expected -1 with -U\n"); // not sure about this, please fix if ( args.aux.flag & CALL_CONSTR_ALLELES ) { if ( !args.targets ) error("Expected -t or -T with \"-C alleles\"\n"); if ( !(args.flag & CF_MCALL) ) error("The \"-C alleles\" mode requires -m\n"); } if ( args.aux.flag & CALL_CHR_X && args.aux.flag & CALL_CHR_Y ) error("Only one of -X or -Y should be given\n"); if ( args.flag & CF_INS_MISSED && !(args.aux.flag&CALL_CONSTR_ALLELES) ) error("The -i option requires -C alleles\n"); init_data(&args); while ( bcf_sr_next_line(args.aux.srs) ) { bcf1_t *bcf_rec = args.aux.srs->readers[0].buffer[0]; if ( args.samples_map ) bcf_subset(args.aux.hdr, bcf_rec, args.nsamples, args.samples_map); bcf_unpack(bcf_rec, BCF_UN_STR); // Skip unwanted sites if ( args.aux.flag & CALL_VARONLY ) { int is_ref = 0; if ( bcf_rec->n_allele==1 ) is_ref = 1; // not a variant else if ( bcf_rec->n_allele==2 ) { // second allele is mpileup's X, not a variant if ( bcf_rec->d.allele[1][0]=='X' ) is_ref = 1; else if ( bcf_rec->d.allele[1][0]=='<' && bcf_rec->d.allele[1][1]=='X' && bcf_rec->d.allele[1][2]=='>' ) is_ref = 1; else if ( bcf_rec->d.allele[1][0]=='<' && bcf_rec->d.allele[1][1]=='*' && bcf_rec->d.allele[1][2]=='>' ) is_ref = 1; } if ( is_ref ) { // gVCF output if ( args.flag & CF_GVCF ) gvcf_write(args.out_fh, &args.gvcf, args.aux.hdr, bcf_rec, 1); continue; } } if ( (args.flag & CF_INDEL_ONLY) && bcf_is_snp(bcf_rec) ) continue; // not an indel if ( (args.flag & CF_NO_INDEL) && !bcf_is_snp(bcf_rec) ) continue; // not a SNP if ( (args.flag & CF_ACGT_ONLY) && (bcf_rec->d.allele[0][0]=='N' || bcf_rec->d.allele[0][0]=='n') ) continue; // REF[0] is 'N' bcf_unpack(bcf_rec, BCF_UN_ALL); // Various output modes: QCall output (todo) if ( args.flag & CF_QCALL ) { qcall(&args.aux, bcf_rec); continue; } // Calling modes which output VCFs int ret; if ( args.flag & CF_MCALL ) ret = mcall(&args.aux, bcf_rec); else ret = ccall(&args.aux, bcf_rec); if ( ret==-1 ) error("Something is wrong\n"); // gVCF output if ( args.flag & CF_GVCF ) { gvcf_write(args.out_fh, &args.gvcf, args.aux.hdr, bcf_rec, ret?0:1); continue; } // Normal output if ( (args.aux.flag & CALL_VARONLY) && ret==0 ) continue; // not a variant bcf_write1(args.out_fh, args.aux.hdr, bcf_rec); } if ( args.flag & CF_GVCF ) gvcf_write(args.out_fh, &args.gvcf, args.aux.hdr, NULL, 0); if ( args.flag & CF_INS_MISSED ) bcf_sr_regions_flush(args.aux.srs->targets); destroy_data(&args); return 0; } bcftools-1.2/vcfcnv.c000066400000000000000000001127431246371514100146340ustar00rootroot00000000000000/* The MIT License Copyright (c) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include "bcftools.h" #include "HMM.h" #define DBG_HMM_PRN 0 #define N_STATES 5 #define CN0 0 #define CN1 1 #define CN2 2 #define CN3 3 #define CNx 4 typedef struct { char *name; int idx; double pobs[N_STATES]; FILE *dat_fh, *cn_fh, *summary_fh; char *dat_fname, *cn_fname, *summary_fname; } sample_t; typedef struct _args_t { bcf_srs_t *files; bcf_hdr_t *hdr; int prev_rid, ntot, nused; sample_t query_sample, control_sample; int nstates; // number of states: N_STATES for one sample, N_STATES^2 for two samples double baf_sigma2, lrr_sigma2; // squared std dev of B-allele frequency and LRR distribution double lrr_bias, baf_bias; // LRR/BAF weights double same_prob, ij_prob; // prior of both samples being the same and the transition probability P(i|j) double err_prob; // constant probability of erroneous measurement double pRR, pRA, pAA, pRR_dflt, pRA_dflt, pAA_dflt; double *tprob, *tprob_arr; // array of transition matrices, precalculated up to ntprob_arr positions int ntprob_arr; hmm_t *hmm; double *eprob; // emission probs [nstates*nsites,msites] uint32_t *sites; // positions [nsites,msites] int nsites, msites; double baum_welch_th; float plot_th; FILE *summary_fh; char **argv, *regions_list, *summary_fname, *output_dir; char *targets_list, *af_fname; int argc; } args_t; FILE *open_file(char **fname, const char *mode, const char *fmt, ...); static inline void hmm2cn_state(int nstates, int i, int *a, int *b) { *a = i / N_STATES; *b = i - (*a)*N_STATES; } static double *init_tprob_matrix(int ndim, double ij_prob, double same_prob) { int i,j; double *mat = (double*) malloc(sizeof(double)*ndim*ndim); assert( ndim==N_STATES || ndim==N_STATES*N_STATES); double pii = 1 - ij_prob*(N_STATES-1); if ( ndim==N_STATES ) // one sample { if ( pii < ij_prob ) error("Error: -x set a bit too high, P(x|x) < P(x|y): %e vs %e\n", pii,ij_prob); for (j=0; jdat_fh = open_file(&smpl->dat_fname,"w","%s/dat.%s.tab",dir,smpl->name); smpl->cn_fh = open_file(&smpl->cn_fname,"w","%s/cn.%s.tab",dir,smpl->name); smpl->summary_fh = open_file(&smpl->summary_fname,"w","%s/summary.%s.tab",dir,smpl->name); fprintf(smpl->dat_fh,"# [1]Chromosome\t[2]Position\t[3]BAF\t[4]LRR\n"); fprintf(smpl->cn_fh,"# [1]Chromosome\t[2]Position\t[3]CN\t[4]P(CN0)\t[5]P(CN1)\t[6]P(CN2)\t[7]P(CN3)\t[8]P(CNx)\n"); fprintf(smpl->summary_fh,"# RG, Regions [2]Chromosome\t[3]Start\t[4]End\t[5]Copy Number state\t[6]Quality\n"); } static void close_sample_files(sample_t *smpl) { fclose(smpl->dat_fh); fclose(smpl->cn_fh); fclose(smpl->summary_fh); } static void init_data(args_t *args) { args->prev_rid = -1; args->hdr = args->files->readers[0].header; if ( !args->query_sample.name ) { if ( bcf_hdr_nsamples(args->hdr)>1 ) error("Multi-sample VCF, missing the -s option\n"); args->query_sample.name = strdup(args->hdr->samples[0]); } else if ( bcf_hdr_id2int(args->hdr,BCF_DT_SAMPLE,args->query_sample.name)<0 ) error("The sample \"%s\" not found\n", args->query_sample.name); if ( !args->files->readers[0].file->is_bin ) { int ret; kstring_t tmp = {0,0,0}; if ( args->control_sample.name ) { ksprintf(&tmp, "%s,%s", args->query_sample.name,args->control_sample.name); ret = bcf_hdr_set_samples(args->hdr, tmp.s, 0); } else { ret = bcf_hdr_set_samples(args->hdr, args->query_sample.name, 0); tmp.s = args->query_sample.name; } if ( ret<0 ) error("Error parsing the list of samples: %s\n", tmp.s); else if ( ret>0 ) error("The sample not found in the VCF: %s\n", ret==1 ? args->query_sample.name : args->control_sample.name); if ( args->control_sample.name ) free(tmp.s); } args->query_sample.idx = bcf_hdr_id2int(args->hdr,BCF_DT_SAMPLE,args->query_sample.name); args->control_sample.idx = args->control_sample.name ? bcf_hdr_id2int(args->hdr,BCF_DT_SAMPLE,args->control_sample.name) : -1; args->nstates = args->control_sample.name ? N_STATES*N_STATES : N_STATES; args->tprob = init_tprob_matrix(args->nstates, args->ij_prob, args->same_prob); args->hmm = hmm_init(args->nstates, args->tprob, 10000); args->summary_fh = stdout; if ( args->output_dir ) { init_sample_files(&args->query_sample, args->output_dir); if ( args->control_sample.name ) { init_sample_files(&args->control_sample, args->output_dir); args->summary_fh = open_file(&args->summary_fname,"w","%s/summary.tab",args->output_dir); } else args->summary_fh = NULL; // one sample only, no two-file summary } int i; FILE *fh = args->summary_fh ? args->summary_fh : args->query_sample.summary_fh; fprintf(fh, "# This file was produced by: bcftools cnv(%s+htslib-%s)\n", bcftools_version(),hts_version()); fprintf(fh, "# The command line was:\tbcftools %s", args->argv[0]); for (i=1; iargc; i++) fprintf(fh, " %s",args->argv[i]); if ( args->control_sample.name ) fprintf(fh, "\n#\n" "# RG, Regions\t[2]Chromosome\t[3]Start\t[4]End\t[5]Copy number:%s\t[6]Copy number:%s\t[7]Quality\n", args->query_sample.name,args->control_sample.name ); else fprintf(fh, "\n#\n" "# RG, Regions\t[2]Chromosome\t[3]Start\t[4]End\t[5]Copy number:%s\t[6]Quality\n", args->query_sample.name ); } char *msprintf(const char *fmt, ...); static void py_plot_cnv(char *script, float th) { if ( th>100 ) return; // create no plots char *cmd = msprintf("python %s -p %f", script, th); int ret = system(cmd); if ( ret) fprintf(stderr, "The command returned non-zero status %d: %s\n", ret, cmd); free(cmd); } static void plot_sample(args_t *args, sample_t *smpl) { char *fname; FILE *fp = open_file(&fname,"w","%s/plot.%s.py",args->output_dir,smpl->name); fprintf(fp, "import matplotlib as mpl\n" "mpl.use('Agg')\n" "import matplotlib.pyplot as plt\n" "import csv\n" "import numpy as np\n" "csv.register_dialect('tab', delimiter='\\t', quoting=csv.QUOTE_NONE)\n" "\n" "dat = {}\n" "with open('%s', 'rb') as f:\n" " reader = csv.reader(f, 'tab')\n" " for row in reader:\n" " chr = row[0]\n" " if chr[0]=='#': continue\n" " if chr not in dat: dat[chr] = []\n" " dat[chr].append([row[1], float(row[2]), float(row[3])])\n" "\n" "cnv = {}\n" "with open('%s', 'rb') as f:\n" " reader = csv.reader(f, 'tab')\n" " for row in reader:\n" " chr = row[0]\n" " if chr[0]=='#': continue\n" " if chr not in cnv: cnv[chr] = []\n" " row[2] = int(row[2]) + 0.5\n" " cnv[chr].append(row[1:])\n" "\n" "for chr in dat:\n" " fig,(ax1, ax2, ax3) = plt.subplots(3,1,figsize=(10,8),sharex=True)\n" " ax1.plot([x[0] for x in dat[chr]],[x[2] for x in dat[chr]], '.', ms=3)\n" " ax2.plot([x[0] for x in dat[chr]],[x[1] for x in dat[chr]], '.', ms=3)\n" " cn_dat = cnv[chr]\n" " xgrid = [float(x[0]) for x in cn_dat]\n" " ygrid = np.linspace(0,5,6)\n" " xgrid, ygrid = np.meshgrid(xgrid, ygrid)\n" " heat = np.zeros_like(xgrid)\n" " for x in range(len(heat[0])-1):\n" " heat[0][x] = cn_dat[x][2]\n" " heat[1][x] = cn_dat[x][3]\n" " heat[2][x] = cn_dat[x][4]\n" " heat[3][x] = cn_dat[x][5]\n" " heat[4][x] = cn_dat[x][6]\n" " mesh = ax3.pcolormesh(xgrid, ygrid, heat, cmap='bwr_r')\n" " mesh.set_clim(vmin=-1,vmax=1)\n" " ax3.plot([x[0] for x in cn_dat],[x[1] for x in cn_dat],'.-',ms=3,color='black')\n" " fig.suptitle('%s (chr '+chr+')')\n" " ax1.set_title('Log-R intensities Ratio',fontsize=10)\n" " ax2.set_title('B-Allele Frequency',fontsize=10)\n" " ax3.set_title('Copy Number Variation',fontsize=10)\n" " ax1.set_ylabel('LRR')\n" " ax2.set_ylabel('BAF')\n" " ax3.set_ylabel('CN')\n" " ax3.set_xlabel('Coordinate (chrom '+chr+')',fontsize=10)\n" " ax3.set_ylim(-0.1,5.1)\n" " ax3.set_yticks([0.5,1.5,2.5,3.5,4.5])\n" " ax3.set_yticklabels(['CN0','CN1','CN2','CN3','CN4'])\n" " plt.subplots_adjust(left=0.08,right=0.95,bottom=0.08,top=0.92)\n" " plt.savefig('%s/plot.%s.chr'+chr+'.png')\n" " plt.close()\n" "\n", smpl->dat_fname,smpl->cn_fname,smpl->name,args->output_dir,smpl->name ); fclose(fp); py_plot_cnv(fname, args->plot_th); free(fname); } static void create_plots(args_t *args) { close_sample_files(&args->query_sample); if ( args->control_sample.name ) close_sample_files(&args->control_sample); if ( args->summary_fh ) fclose(args->summary_fh); if ( !args->control_sample.name ) { plot_sample(args, &args->query_sample); return; } char *fname; FILE *fp = open_file(&fname,"w","%s/plot.%s.%s.py",args->output_dir,args->control_sample.name,args->query_sample.name); fprintf(fp, "import matplotlib as mpl\n" "mpl.use('Agg')\n" "import matplotlib.pyplot as plt\n" "import csv,argparse\n" "import numpy as np\n" "csv.register_dialect('tab', delimiter='\\t', quoting=csv.QUOTE_NONE)\n" "\n" "control_sample = '%s'\n" "query_sample = '%s'\n" "\n" "parser = argparse.ArgumentParser()\n" "parser.add_argument('-p', '--plot-threshold', type=float)\n" "parser.add_argument('-c', '--chromosome')\n" "args = parser.parse_args()\n" "if args.plot_threshold==None: args.plot_threshold = 0\n" "\n" "def chroms_to_plot(th):\n" " dat = {}\n" " with open('%s/summary.tab', 'rb') as f:\n" " reader = csv.reader(f, 'tab')\n" " for row in reader:\n" " if row[0]!='RG': continue\n" " chr = row[1]\n" " start = row[2]\n" " end = row[3]\n" " qual = float(row[6])\n" " if row[4]==row[5] and args.plot_threshold!=0: continue\n" " if chr not in dat: dat[chr] = 0.0\n" " if qual > dat[chr]: dat[chr] = qual\n" " out = {}\n" " for chr in dat:\n" " if (chr not in dat) or dat[chr]0: diff.append([b[i-1][0],b[i-1][1],a[i-1][1]])\n" " diff.append([b[i][0],b[i][1],a[i][1]])\n" " elif len(diff):\n" " diff.append([b[i][0],b[i][1],a[i][1]])\n" " out.append(diff)\n" " diff = []\n" " if len(diff): out.append(diff)\n" " return out\n" "\n" "control_dat = {}\n" "control_cnv = {}\n" "query_dat = {}\n" "query_cnv = {}\n" "read_dat('%s',control_dat)\n" "read_dat('%s',query_dat)\n" "read_cnv('%s',control_cnv)\n" "read_cnv('%s',query_cnv)\n" "\n" "for chr in query_dat:\n" " fig,(ax1,ax2,ax3,ax4,ax5,ax6) = plt.subplots(6,1,figsize=(10,8),sharex=True)\n" " ax1.plot([x[0] for x in control_dat[chr]],[x[2] for x in control_dat[chr]], '.', ms=3,color='red')\n" " ax2.plot([x[0] for x in control_dat[chr]],[x[1] for x in control_dat[chr]], '.', ms=3,color='red')\n" " cn_dat = control_cnv[chr]\n" " xgrid = [float(x[0]) for x in cn_dat]\n" " ygrid = np.linspace(0,5,6)\n" " xgrid, ygrid = np.meshgrid(xgrid, ygrid)\n" " heat = np.zeros_like(xgrid)\n" " for x in range(len(heat[0])-1):\n" " heat[0][x] = cn_dat[x][2]\n" " heat[1][x] = cn_dat[x][3]\n" " heat[2][x] = cn_dat[x][4]\n" " heat[3][x] = cn_dat[x][5]\n" " heat[4][x] = cn_dat[x][6]\n" " mesh = ax3.pcolormesh(xgrid, ygrid, heat, cmap='bwr')\n" " mesh.set_clim(vmin=-1,vmax=1)\n" " ax3.plot([x[0] for x in cn_dat],[x[1] for x in cn_dat],'-',ms=3,color='black',lw=1.7)\n" "\n" " ax6.plot([x[0] for x in query_dat[chr]],[x[2] for x in query_dat[chr]], '.', ms=3)\n" " ax5.plot([x[0] for x in query_dat[chr]],[x[1] for x in query_dat[chr]], '.', ms=3)\n" " cn_dat = query_cnv[chr]\n" " xgrid = [float(x[0]) for x in cn_dat]\n" " ygrid = np.linspace(0,5,6)\n" " xgrid, ygrid = np.meshgrid(xgrid, ygrid)\n" " heat = np.zeros_like(xgrid)\n" " for x in range(len(heat[0])-1):\n" " heat[0][x] = cn_dat[x][2]\n" " heat[1][x] = cn_dat[x][3]\n" " heat[2][x] = cn_dat[x][4]\n" " heat[3][x] = cn_dat[x][5]\n" " heat[4][x] = cn_dat[x][6]\n" " mesh = ax4.pcolormesh(xgrid, ygrid, heat, cmap='bwr_r')\n" " mesh.set_clim(vmin=-1,vmax=1)\n" " ax4.plot([x[0] for x in cn_dat],[x[1] for x in cn_dat],'-',ms=3,color='black',lw=1.7)\n" " ax3.annotate(control_sample, xy=(0.02,0.1), xycoords='axes fraction', color='red',fontsize=12, va='bottom',ha='left')\n" " ax4.annotate(query_sample, xy=(0.02,0.9), xycoords='axes fraction', color='blue',fontsize=12, va='top',ha='left')\n" "\n" " diffs = find_diffs(control_cnv[chr],query_cnv[chr])\n" " for diff in diffs:\n" " ax3.plot([x[0] for x in diff],[x[1] for x in diff],'-',ms=3,color='blue',lw=1.7)\n" " ax4.plot([x[0] for x in diff],[x[2] for x in diff],'-',ms=3,color='red',lw=1.7)\n" "\n" " fig.suptitle('chr '+chr+', '+control_sample+' vs '+query_sample)\n" " ax1.tick_params(axis='both', labelsize=8)\n" " ax2.tick_params(axis='both', labelsize=8)\n" " ax3.tick_params(axis='both', labelsize=8)\n" " ax4.tick_params(axis='both', labelsize=8)\n" " ax5.tick_params(axis='both', labelsize=8)\n" " ax6.tick_params(axis='both', labelsize=8)\n" " ax6.set_xlabel('Coordinate (chrom '+chr+')',fontsize=8)\n" " ax1.set_ylabel('LRR')\n" " ax2.set_ylabel('BAF')\n" " ax3.set_ylabel('CN')\n" " ax6.set_ylabel('LRR')\n" " ax5.set_ylabel('BAF')\n" " ax4.set_ylabel('CN')\n" " ax3.set_ylim(-0.1,5.1)\n" " ax3.set_yticks([0.5,1.5,2.5,3.5,4.5])\n" " ax3.set_yticklabels(['CN0','CN1','CN2','CN3','CN4'])\n" " ax4.set_ylim(-0.1,5.1)\n" " ax4.set_yticks([0.5,1.5,2.5,3.5,4.5])\n" " ax4.set_yticklabels(['CN0','CN1','CN2','CN3','CN4'])\n" " plt.subplots_adjust(left=0.08,right=0.95,bottom=0.08,top=0.92,hspace=0)\n" " plt.savefig('%s/plot.%s.%s.chr'+chr+'.png')\n" " plt.close()\n" "\n", args->control_sample.name,args->query_sample.name, args->output_dir, args->control_sample.dat_fname,args->query_sample.dat_fname, args->control_sample.cn_fname,args->query_sample.cn_fname, args->output_dir,args->control_sample.name,args->query_sample.name ); fclose(fp); py_plot_cnv(fname,args->plot_th); free(fname); } static void destroy_data(args_t *args) { bcf_sr_destroy(args->files); hmm_destroy(args->hmm); free(args->sites); free(args->eprob); free(args->tprob); free(args->summary_fname); free(args->query_sample.name); free(args->query_sample.dat_fname); free(args->query_sample.cn_fname); free(args->query_sample.summary_fname); free(args->control_sample.dat_fname); free(args->control_sample.cn_fname); free(args->control_sample.summary_fname); } static inline char copy_number_state(args_t *args, int istate, int ismpl) { char code[] = "01234"; if ( !args->control_sample.name ) return code[istate]; int idx = ismpl ? istate - (istate/N_STATES)*N_STATES : istate/N_STATES; return code[idx]; } static double phred_score(double prob) { if ( prob==0 ) return 99; prob = -4.3429*log(prob); return prob>99 ? 99 : prob; } static double avg_ii_prob(int n, double *mat) { int i; double avg = 0; for (i=0; insites ) return; hmm_t *hmm = args->hmm; hmm_set_tprob(args->hmm, args->tprob, 10000); while ( args->baum_welch_th!=0 ) { double ori_ii = avg_ii_prob(hmm->nstates,hmm->tprob_arr); hmm_run_baum_welch(hmm, args->nsites, args->eprob, args->sites); double new_ii = avg_ii_prob(hmm->nstates,hmm->tprob_arr); fprintf(stderr,"%e\t%e\t%e\n", ori_ii,new_ii,new_ii-ori_ii); double *tprob = init_tprob_matrix(hmm->nstates, 1-new_ii, args->same_prob); hmm_set_tprob(args->hmm, tprob, 10000); free(tprob); if ( fabs(new_ii - ori_ii) < args->baum_welch_th ) { int i,j; for (i=0; instates; i++) { for (j=0; jnstates; j++) { printf(" %.15f", MAT(hmm->tprob_arr,hmm->nstates,j,i)); } printf("\n"); } break; } } hmm_run_viterbi(hmm, args->nsites, args->eprob, args->sites); hmm_run_fwd_bwd(hmm, args->nsites, args->eprob, args->sites); // Output the results double qual = 0; int i,j, isite, start_cn = hmm->vpath[0], start_pos = args->sites[0], istart_pos = 0; for (isite=0; isitensites; isite++) { int state = hmm->vpath[args->nstates*isite]; double *pval = hmm->fwd + isite*args->nstates; qual += pval[start_cn]; // output CN and fwd-bwd likelihood for each site if ( args->query_sample.cn_fh ) { fprintf(args->query_sample.cn_fh, "%s\t%d\t%c", bcf_hdr_id2name(args->hdr,args->prev_rid), args->sites[isite]+1, copy_number_state(args,state,0)); if ( !args->control_sample.cn_fh ) for (i=0; instates; i++) fprintf(args->query_sample.cn_fh, "\t%f", pval[i]); else for (i=0; iquery_sample.cn_fh, "\t%f", sum); } fprintf(args->query_sample.cn_fh, "\n"); } if ( args->control_sample.cn_fh ) { fprintf(args->control_sample.cn_fh, "%s\t%d\t%c", bcf_hdr_id2name(args->hdr,args->prev_rid), args->sites[isite]+1, copy_number_state(args,state,1)); for (i=0; icontrol_sample.cn_fh, "\t%f", sum); } fprintf(args->control_sample.cn_fh, "\n"); } if ( start_cn != state ) { char start_cn_query = copy_number_state(args,start_cn,0); qual = phred_score(1 - qual/(isite - istart_pos)); fprintf(args->query_sample.summary_fh,"RG\t%s\t%d\t%d\t%c\t%.1f\n",bcf_hdr_id2name(args->hdr,args->prev_rid), start_pos+1, args->sites[isite],start_cn_query,qual); if ( args->control_sample.name ) { // regions 0-based, half-open char start_cn_ctrl = copy_number_state(args,start_cn,1); fprintf(args->control_sample.summary_fh,"RG\t%s\t%d\t%d\t%c\t%.1f\n",bcf_hdr_id2name(args->hdr,args->prev_rid), start_pos+1, args->sites[isite],start_cn_ctrl,qual); fprintf(args->summary_fh,"RG\t%s\t%d\t%d\t%c\t%c\t%.1f\n",bcf_hdr_id2name(args->hdr,args->prev_rid), start_pos+1, args->sites[isite],start_cn_query,start_cn_ctrl,qual); } istart_pos = isite; start_pos = args->sites[isite]; start_cn = state; qual = 0; } } qual = phred_score(1 - qual/(isite - istart_pos)); char start_cn_query = copy_number_state(args,start_cn,0); fprintf(args->query_sample.summary_fh,"RG\t%s\t%d\t%d\t%c\t%.1f\n",bcf_hdr_id2name(args->hdr,args->prev_rid), start_pos+1, args->sites[isite-1]+1,start_cn_query,qual); if ( args->control_sample.name ) { char start_cn_ctrl = copy_number_state(args,start_cn,1); fprintf(args->control_sample.summary_fh,"RG\t%s\t%d\t%d\t%c\t%.1f\n",bcf_hdr_id2name(args->hdr,args->prev_rid), start_pos+1, args->sites[isite-1]+1,start_cn_ctrl,qual); fprintf(args->summary_fh,"RG\t%s\t%d\t%d\t%c\t%c\t%.1f\n",bcf_hdr_id2name(args->hdr,args->prev_rid), start_pos+1, args->sites[isite-1]+1,start_cn_query,start_cn_ctrl,qual); } } static int set_observed_prob(args_t *args, bcf_fmt_t *baf_fmt, bcf_fmt_t *lrr_fmt, sample_t *smpl) { float baf, lrr; baf = ((float*)(baf_fmt->p + baf_fmt->size*smpl->idx))[0]; if ( bcf_float_is_missing(baf) || isnan(baf) ) baf = -0.1; // arbitrary negative value == missing value lrr = ((float*)(lrr_fmt->p + lrr_fmt->size*smpl->idx))[0]; if ( bcf_float_is_missing(lrr) || isnan(lrr) ) baf = -0.1; if ( baf>=0 ) // skip missing values fprintf(smpl->dat_fh,"%s\t%d\t%.3f\t%.3f\n",bcf_hdr_id2name(args->hdr,args->prev_rid), args->sites[args->nsites-1]+1,baf,lrr); if ( baf<0 ) { // no call: either some technical issue or the call could not be made because it is CN0 int i; smpl->pobs[CN0] = 0.5; for (i=1; ipobs[i] = (1.0-smpl->pobs[CN0])/(N_STATES-1); return 0; } double pk0, pk14, pk13, pk12, pk23, pk34, pk1; pk0 = exp(-baf*baf/args->baf_sigma2); pk14 = exp(-(baf-1/4.)*(baf-1/4.)/args->baf_sigma2); pk13 = exp(-(baf-1/3.)*(baf-1/3.)/args->baf_sigma2); pk12 = exp(-(baf-1/2.)*(baf-1/2.)/args->baf_sigma2); pk23 = exp(-(baf-2/3.)*(baf-2/3.)/args->baf_sigma2); pk34 = exp(-(baf-3/4.)*(baf-3/4.)/args->baf_sigma2); pk1 = exp(-(baf-1.0)*(baf-1.0)/args->baf_sigma2); double cn1_baf, cn2_baf, cn3_baf, cn4_baf; cn1_baf = pk0*(args->pRR+args->pRA/2.) + pk1*(args->pAA+args->pRA/2.); cn2_baf = pk0*args->pRR + pk1*args->pAA + pk12*args->pRA; cn3_baf = pk0*args->pRR + pk1*args->pAA + (pk13 + pk23)*args->pRA/2.; cn4_baf = pk0*args->pRR + pk1*args->pAA + (pk14 + pk12 + pk34)*args->pRA/3.; double cn1_lrr, cn2_lrr, cn3_lrr, cn4_lrr; cn1_lrr = exp(-(lrr + 0.45)*(lrr + 0.45)/args->lrr_sigma2); cn2_lrr = exp(-(lrr - 0.00)*(lrr - 0.00)/args->lrr_sigma2); cn3_lrr = exp(-(lrr - 0.30)*(lrr - 0.30)/args->lrr_sigma2); cn4_lrr = exp(-(lrr - 0.75)*(lrr - 0.75)/args->lrr_sigma2); smpl->pobs[CN0] = 0; smpl->pobs[CN1] = args->err_prob + (1 - args->baf_bias + args->baf_bias*cn1_baf)*(1 - args->lrr_bias + args->lrr_bias*cn1_lrr); smpl->pobs[CN2] = args->err_prob + (1 - args->baf_bias + args->baf_bias*cn2_baf)*(1 - args->lrr_bias + args->lrr_bias*cn2_lrr); smpl->pobs[CN3] = args->err_prob + (1 - args->baf_bias + args->baf_bias*cn3_baf)*(1 - args->lrr_bias + args->lrr_bias*cn3_lrr); smpl->pobs[CNx] = args->err_prob + (1 - args->baf_bias + args->baf_bias*cn4_baf)*(1 - args->lrr_bias + args->lrr_bias*cn4_lrr); return 0; } static void set_emission_prob(args_t *args) { double *eprob = &args->eprob[args->nstates*(args->nsites-1)]; int i; for (i=0; iquery_sample.pobs[i]; } static void set_emission_prob2(args_t *args) { double *eprob = &args->eprob[args->nstates*(args->nsites-1)]; int i, j; for (i=0; iquery_sample.pobs[i]*args->control_sample.pobs[j]; } } } int read_AF(bcf_sr_regions_t *tgt, bcf1_t *line, double *alt_freq); static void cnv_next_line(args_t *args, bcf1_t *line) { if ( !line ) { // Done, flush viterbi cnv_flush_viterbi(args); return; } if ( line->rid!=args->prev_rid ) { // New chromosome cnv_flush_viterbi(args); args->prev_rid = line->rid; args->nsites = 0; } // Process line args->ntot++; bcf_fmt_t *baf_fmt, *lrr_fmt; if ( !(baf_fmt = bcf_get_fmt(args->hdr, line, "BAF")) ) return; if ( !(lrr_fmt = bcf_get_fmt(args->hdr, line, "LRR")) ) return; // Realloc buffers needed by viterbi and fwd-bwd args->nsites++; int m = args->msites; hts_expand(uint32_t,args->nsites,args->msites,args->sites); if ( args->msites!=m ) args->eprob = (double*) realloc(args->eprob,sizeof(double)*args->msites*args->nstates); args->sites[args->nsites-1] = line->pos; double alt_freq; if ( !args->af_fname || read_AF(args->files->targets, line, &alt_freq) < 0 ) { args->pRR = args->pRR_dflt; args->pRA = args->pRA_dflt; args->pAA = args->pAA_dflt; } else { args->pRR = (1 - alt_freq)*(1 - alt_freq); args->pRA = 2*(1 - alt_freq)*alt_freq; args->pAA = alt_freq*alt_freq; } int ret = set_observed_prob(args, baf_fmt,lrr_fmt, &args->query_sample); if ( ret<0 ) { args->nsites--; return; } if ( args->control_sample.name ) { ret = set_observed_prob(args, baf_fmt,lrr_fmt, &args->control_sample); if ( ret<0 ) { args->nsites--; return; } } if ( args->control_sample.name ) set_emission_prob2(args); else set_emission_prob(args); args->nused++; } static void usage(args_t *args) { fprintf(stderr, "\n"); fprintf(stderr, "About: Copy number variation caller, requires Illumina's B-allele frequency (BAF) and Log R\n"); fprintf(stderr, " Ratio intensity (LRR). The HMM considers the following copy number states: CN 2\n"); fprintf(stderr, " (normal), 1 (single-copy loss), 0 (complete loss), 3 (single-copy gain), x (other)\n"); fprintf(stderr, "Usage: bcftools cnv [OPTIONS] \n"); fprintf(stderr, "General Options:\n"); fprintf(stderr, " -c, --control-sample optional control sample name to highlight differences\n"); fprintf(stderr, " -f, --AF-file read allele frequencies from file (CHR\\tPOS\\tREF,ALT\\tAF)\n"); fprintf(stderr, " -o, --output-dir \n"); fprintf(stderr, " -p, --plot-threshold plot aberrant chromosomes with quality at least 'float'\n"); fprintf(stderr, " -r, --regions restrict to comma-separated list of regions\n"); fprintf(stderr, " -R, --regions-file restrict to regions listed in a file\n"); fprintf(stderr, " -s, --query-sample query samply name\n"); fprintf(stderr, " -t, --targets similar to -r but streams rather than index-jumps\n"); fprintf(stderr, " -T, --targets-file similar to -R but streams rather than index-jumps\n"); fprintf(stderr, "HMM Options:\n"); fprintf(stderr, " -b, --BAF-weight relative contribution from BAF [1]\n"); fprintf(stderr, " -e, --err-prob probability of error [1e-4]\n"); fprintf(stderr, " -l, --LRR-weight relative contribution from LRR [0.2]\n"); fprintf(stderr, " -P, --same-prob prior probability of -s/-c being same [1e-1]\n"); fprintf(stderr, " -x, --xy-prob P(x|y) transition probability [1e-8]\n"); fprintf(stderr, "\n"); exit(1); } int main_vcfcnv(int argc, char *argv[]) { int c; args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->argc = argc; args->argv = argv; args->files = bcf_sr_init(); args->plot_th = 1e9; // by default plot none // How much FORMAT/LRR and FORMAT/BAF matter args->lrr_bias = 0.2; args->baf_bias = 1.0; args->err_prob = 1e-4; // Transition probability to a different state and the prior of both samples being the same args->ij_prob = 1e-8; args->same_prob = 1e-1; // Squared std dev of BAF and LRR values (gaussian noise), estimated from real data (hets, one sample, one chr) args->baf_sigma2 = 0.08*0.08; // illumina: 0.03 args->lrr_sigma2 = 0.4*0.4; //0.20*0.20; // illumina: 0.18 // Priors for RR, RA, AA genotypes args->pRR_dflt = 0.76; args->pRA_dflt = 0.14; args->pAA_dflt = 0.098; // args->pRR = 0.69; // args->pRA = 0.18; // args->pAA = 0.11; int regions_is_file = 0, targets_is_file = 0; static struct option loptions[] = { {"AF-file",1,0,'f'}, {"baum-welch",1,0,'W'}, {"err-prob",1,0,'e'}, {"BAF-weight",1,0,'b'}, {"LRR-weight",1,0,'l'}, {"same-prob",1,0,'P'}, {"xy-prob",1,0,'x'}, {"sample",1,0,'s'}, {"control",1,0,'c'}, {"targets",1,0,'t'}, {"targets-file",1,0,'T'}, {"regions",1,0,'r'}, {"regions-file",1,0,'R'}, {"plot",1,0,'p'}, {"output-dir",1,0,'o'}, {0,0,0,0} }; char *tmp = NULL; while ((c = getopt_long(argc, argv, "h?r:R:t:T:s:o:p:l:T:c:b:P:x:e:W:f:",loptions,NULL)) >= 0) { switch (c) { case 'f': args->af_fname = optarg; break; case 'W': args->baum_welch_th = strtod(optarg,&tmp); if ( *tmp ) error("Could not parse: -W %s\n", optarg); break; case 'e': args->err_prob = strtod(optarg,&tmp); if ( *tmp ) error("Could not parse: -e %s\n", optarg); break; case 'b': args->baf_bias = strtod(optarg,&tmp); if ( *tmp ) error("Could not parse: -b %s\n", optarg); break; case 'x': args->ij_prob = strtod(optarg,&tmp); if ( *tmp ) error("Could not parse: -x %s\n", optarg); break; case 'P': args->same_prob = strtod(optarg,&tmp); if ( *tmp ) error("Could not parse: -P %s\n", optarg); break; case 'l': args->lrr_bias = strtod(optarg,&tmp); if ( *tmp ) error("Could not parse: -l %s\n", optarg); break; case 'p': args->plot_th = strtod(optarg,&tmp); if ( *tmp ) error("Could not parse: -p %s\n", optarg); break; case 'o': args->output_dir = optarg; break; case 's': args->query_sample.name = strdup(optarg); break; case 'c': args->control_sample.name = optarg; break; case 't': args->targets_list = optarg; break; case 'T': args->targets_list = optarg; targets_is_file = 1; break; case 'r': args->regions_list = optarg; break; case 'R': args->regions_list = optarg; regions_is_file = 1; break; case 'h': case '?': usage(args); break; default: error("Unknown argument: %s\n", optarg); } } char *fname = NULL; if ( optind>=argc ) { if ( !isatty(fileno((FILE *)stdin)) ) fname = "-"; } else fname = argv[optind]; if ( !fname ) usage(args); if ( args->plot_th<=100 && !args->output_dir ) error("Expected -o option with -p\n"); if ( args->regions_list ) { if ( bcf_sr_set_regions(args->files, args->regions_list, regions_is_file)<0 ) error("Failed to read the regions: %s\n", args->regions_list); } if ( args->targets_list ) { if ( bcf_sr_set_targets(args->files, args->targets_list, targets_is_file, 0)<0 ) error("Failed to read the targets: %s\n", args->targets_list); } if ( args->af_fname ) { if ( bcf_sr_set_targets(args->files, args->af_fname, 1, 3)<0 ) error("Failed to read the targets: %s\n", args->af_fname); } if ( !bcf_sr_add_reader(args->files, fname) ) error("Failed to open %s: %s\n", fname,bcf_sr_strerror(args->files->errnum)); init_data(args); while ( bcf_sr_next_line(args->files) ) { bcf1_t *line = bcf_sr_get_line(args->files,0); cnv_next_line(args, line); } cnv_next_line(args, NULL); create_plots(args); fprintf(stderr,"Number of lines: total/processed: %d/%d\n", args->ntot,args->nused); destroy_data(args); free(args); return 0; } bcftools-1.2/vcfconcat.c000066400000000000000000000635761246371514100153260ustar00rootroot00000000000000/* vcfconcat.c -- Concatenate or combine VCF/BCF files. Copyright (C) 2013-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include "bcftools.h" typedef struct _args_t { bcf_srs_t *files; htsFile *out_fh; int output_type; bcf_hdr_t *out_hdr; int *seen_seq; // phasing int *start_pos, start_tid, ifname; int *swap_phase, nswap, *nmatch, *nmism; bcf1_t **buf; int nbuf, mbuf, prev_chr, min_PQ, prev_pos_check; int32_t *GTa, *GTb, mGTa, mGTb, *phase_qual, *phase_set; char **argv, *output_fname, *file_list, **fnames, *regions_list; int argc, nfnames, allow_overlaps, phased_concat, remove_dups, regions_is_file; } args_t; static void init_data(args_t *args) { bcf1_t *line = NULL; // With phased concat, the chunks overlap and come in the right order. To // avoid opening all files at once, store start positions to recognise need // for the next one. This way we can keep only two open chunks at once. if ( args->phased_concat ) { args->start_pos = (int*) malloc(sizeof(int)*args->nfnames); line = bcf_init(); } kstring_t str = {0,0,0}; int i, prev_chrid = -1; for (i=0; infnames; i++) { htsFile *fp = hts_open(args->fnames[i], "r"); if ( !fp ) error("Failed to open: %s\n", args->fnames[i]); bcf_hdr_t *hdr = bcf_hdr_read(fp); if ( !hdr ) error("Failed to parse header: %s\n", args->fnames[i]); if ( !args->out_hdr ) args->out_hdr = bcf_hdr_dup(hdr); else { bcf_hdr_combine(args->out_hdr, hdr); if ( bcf_hdr_nsamples(hdr) != bcf_hdr_nsamples(args->out_hdr) ) error("Different number of samples in %s. Perhaps \"bcftools merge\" is what you are looking for?\n", args->fnames[i]); int j; for (j=0; jout_hdr->samples[j],hdr->samples[j]) ) error("Different sample names in %s. Perhaps \"bcftools merge\" is what you are looking for?\n", args->fnames[i]); } if ( args->phased_concat ) { int ret = bcf_read(fp, hdr, line); if ( ret!=0 ) args->start_pos[i] = -2; // empty file else { int chrid = bcf_hdr_id2int(args->out_hdr,BCF_DT_CTG,bcf_seqname(hdr,line)); args->start_pos[i] = chrid==prev_chrid ? line->pos : -1; prev_chrid = chrid; } } bcf_hdr_destroy(hdr); hts_close(fp); } free(str.s); if ( line ) bcf_destroy(line); args->seen_seq = (int*) calloc(args->out_hdr->n[BCF_DT_CTG],sizeof(int)); if ( args->phased_concat ) { bcf_hdr_append(args->out_hdr,"##FORMAT="); bcf_hdr_append(args->out_hdr,"##FORMAT="); } bcf_hdr_append_version(args->out_hdr, args->argc, args->argv, "bcftools_concat"); args->out_fh = hts_open(args->output_fname,hts_bcf_wmode(args->output_type)); if ( args->out_fh == NULL ) error("Can't write to \"%s\": %s\n", args->output_fname, strerror(errno)); bcf_hdr_write(args->out_fh, args->out_hdr); if ( args->allow_overlaps ) { args->files = bcf_sr_init(); args->files->require_index = 1; if ( args->regions_list ) { if ( bcf_sr_set_regions(args->files, args->regions_list, args->regions_is_file)<0 ) error("Failed to read the regions: %s\n", args->regions_list); } for (i=0; infnames; i++) if ( !bcf_sr_add_reader(args->files,args->fnames[i]) ) error("Failed to open %s: %s\n", args->fnames[i],bcf_sr_strerror(args->files->errnum)); } else if ( args->phased_concat ) { // Remove empty files from the list int nok = 0; while (1) { while ( noknfnames && args->start_pos[nok]!=-2 ) nok++; if ( nok==args->nfnames ) break; i = nok; while ( infnames && args->start_pos[i]==-2 ) i++; if ( i==args->nfnames ) break; int tmp = args->start_pos[nok]; args->start_pos[nok] = args->start_pos[i]; args->start_pos[i] = tmp; char *str = args->fnames[nok]; args->fnames[nok] = args->fnames[i]; args->fnames[i] = str; } for (i=nok; infnames; i++) free(args->fnames[i]); args->nfnames = nok; for (i=1; infnames; i++) if ( args->start_pos[i-1]!=-1 && args->start_pos[i]!=-1 && args->start_pos[i]start_pos[i-1] ) error("The files not in ascending order: %d in %s, %d in %s\n", args->start_pos[i-1]+1,args->fnames[i-1],args->start_pos[i]+1,args->fnames[i]); args->prev_chr = -1; args->swap_phase = (int*) calloc(bcf_hdr_nsamples(args->out_hdr),sizeof(int)); args->nmatch = (int*) calloc(bcf_hdr_nsamples(args->out_hdr),sizeof(int)); args->nmism = (int*) calloc(bcf_hdr_nsamples(args->out_hdr),sizeof(int)); args->phase_qual = (int32_t*) malloc(bcf_hdr_nsamples(args->out_hdr)*sizeof(int32_t)); args->phase_set = (int32_t*) malloc(bcf_hdr_nsamples(args->out_hdr)*sizeof(int32_t)); args->files = bcf_sr_init(); args->files->require_index = 1; args->ifname = 0; } } static void destroy_data(args_t *args) { int i; for (i=0; infnames; i++) free(args->fnames[i]); free(args->fnames); if ( args->files ) bcf_sr_destroy(args->files); if ( hts_close(args->out_fh)!=0 ) error("hts_close error\n"); bcf_hdr_destroy(args->out_hdr); free(args->seen_seq); free(args->start_pos); free(args->swap_phase); for (i=0; imbuf; i++) bcf_destroy(args->buf[i]); free(args->buf); free(args->GTa); free(args->GTb); free(args->nmatch); free(args->nmism); free(args->phase_qual); free(args->phase_set); } int vcf_write_line(htsFile *fp, kstring_t *line); #define SWAP(type_t, a, b) { type_t t = a; a = b; b = t; } static void phase_update(args_t *args, bcf_hdr_t *hdr, bcf1_t *rec) { int i, nGTs = bcf_get_genotypes(hdr, rec, &args->GTa, &args->mGTa); for (i=0; iswap_phase[i] ) continue; int *gt = &args->GTa[i*2]; if ( bcf_gt_is_missing(gt[0]) || gt[1]==bcf_int32_vector_end ) continue; SWAP(int, gt[0], gt[1]); gt[1] |= 1; } bcf_update_genotypes(hdr,rec,args->GTa,nGTs); } static void phased_flush(args_t *args) { if ( !args->nbuf ) return; bcf_hdr_t *ahdr = args->files->readers[0].header; bcf_hdr_t *bhdr = args->files->readers[1].header; int i, j, nsmpl = bcf_hdr_nsamples(args->out_hdr); for (i=0; inbuf; i+=2) { bcf1_t *arec = args->buf[i]; bcf1_t *brec = args->buf[i+1]; int nGTs = bcf_get_genotypes(ahdr, arec, &args->GTa, &args->mGTa); if ( nGTs < 0 ) error("GT is not present at %s:%d\n", bcf_seqname(ahdr,arec), arec->pos+1); if ( nGTs != 2*nsmpl ) continue; // not diploid nGTs = bcf_get_genotypes(bhdr, brec, &args->GTb, &args->mGTb); if ( nGTs < 0 ) error("GT is not present at %s:%d\n", bcf_seqname(bhdr,brec), brec->pos+1); if ( nGTs != 2*nsmpl ) continue; // not diploid for (j=0; jGTa[j*2]; int *gtb = &args->GTb[j*2]; if ( gta[1]==bcf_int32_vector_end || gtb[1]==bcf_int32_vector_end ) continue; if ( bcf_gt_is_missing(gta[0]) || bcf_gt_is_missing(gta[1]) || bcf_gt_is_missing(gtb[0]) || bcf_gt_is_missing(gtb[1]) ) continue; if ( !bcf_gt_is_phased(gta[1]) || !bcf_gt_is_phased(gtb[1]) ) continue; if ( bcf_gt_allele(gta[0])==bcf_gt_allele(gta[1]) || bcf_gt_allele(gtb[0])==bcf_gt_allele(gtb[1]) ) continue; if ( bcf_gt_allele(gta[0])==bcf_gt_allele(gtb[0]) && bcf_gt_allele(gta[1])==bcf_gt_allele(gtb[1]) ) { if ( args->swap_phase[j] ) args->nmism[j]++; else args->nmatch[j]++; } if ( bcf_gt_allele(gta[0])==bcf_gt_allele(gtb[1]) && bcf_gt_allele(gta[1])==bcf_gt_allele(gtb[0]) ) { if ( args->swap_phase[j] ) args->nmatch[j]++; else args->nmism[j]++; } } } for (i=0; inbuf/2; i+=2) { bcf1_t *arec = args->buf[i]; bcf_translate(args->out_hdr, args->files->readers[0].header, arec); if ( args->nswap ) phase_update(args, args->out_hdr, arec); bcf_update_format_int32(args->out_hdr,arec,"PS",args->phase_set,nsmpl); bcf_write(args->out_fh, args->out_hdr, arec); if ( arec->pos < args->prev_pos_check ) error("FIXME, disorder: %s:%d vs %d [1]\n", bcf_seqname(args->files->readers[0].header,arec),arec->pos+1,args->prev_pos_check+1); args->prev_pos_check = arec->pos; } args->nswap = 0; for (j=0; jnmatch[j] >= args->nmism[j] ) args->swap_phase[j] = 0; else { args->swap_phase[j] = 1; args->nswap++; } if ( args->nmatch[j] && args->nmism[j] ) { // Entropy-inspired quality. The factor 0.7 shifts and scales to (0,1) double f = (double)args->nmatch[j]/(args->nmatch[j]+args->nmism[j]); args->phase_qual[j] = 99*(0.7 + f*log(f) + (1-f)*log(1-f))/0.7; } else args->phase_qual[j] = 99; args->nmatch[j] = 0; args->nmism[j] = 0; } int PQ_printed = 0; for (; inbuf; i+=2) { bcf1_t *brec = args->buf[i+1]; bcf_translate(args->out_hdr, args->files->readers[1].header, brec); if ( !PQ_printed ) { bcf_update_format_int32(args->out_hdr,brec,"PQ",args->phase_qual,nsmpl); PQ_printed = 1; for (j=0; jphase_qual[j] < args->min_PQ ) args->phase_set[j] = brec->pos+1; } if ( args->nswap ) phase_update(args, args->out_hdr, brec); bcf_update_format_int32(args->out_hdr,brec,"PS",args->phase_set,nsmpl); bcf_write(args->out_fh, args->out_hdr, brec); if ( brec->pos < args->prev_pos_check ) error("FIXME, disorder: %s:%d vs %d [2]\n", bcf_seqname(args->files->readers[1].header,brec),brec->pos+1,args->prev_pos_check+1); args->prev_pos_check = brec->pos; } args->nbuf = 0; } static void phased_push(args_t *args, bcf1_t *arec, bcf1_t *brec) { if ( arec && arec->errcode ) error("Parse error at %s:%d, cannot proceed: %s\n", bcf_seqname(args->files->readers[0].header,arec),arec->pos+1, args->files->readers[0].fname); if ( brec && brec->errcode ) error("Parse error at %s:%d, cannot proceed: %s\n", bcf_seqname(args->files->readers[1].header,brec),brec->pos+1, args->files->readers[1].fname); int i, nsmpl = bcf_hdr_nsamples(args->out_hdr); int chr_id = bcf_hdr_name2id(args->out_hdr, bcf_seqname(args->files->readers[0].header,arec)); if ( args->prev_chr<0 || args->prev_chr!=chr_id ) { if ( args->prev_chr>=0 ) phased_flush(args); for (i=0; iphase_set[i] = arec->pos+1; if ( args->seen_seq[chr_id] ) error("The chromosome block %s is not contiguous\n", bcf_seqname(args->files->readers[0].header,arec)); args->seen_seq[chr_id] = 1; args->prev_chr = chr_id; args->prev_pos_check = -1; } if ( !brec ) { bcf_translate(args->out_hdr, args->files->readers[0].header, arec); if ( args->nswap ) phase_update(args, args->out_hdr, arec); bcf_update_format_int32(args->out_hdr,arec,"PS",args->phase_set,nsmpl); bcf_write(args->out_fh, args->out_hdr, arec); if ( arec->pos < args->prev_pos_check ) error("FIXME, disorder: %s:%d in %s vs %d written [3]\n", bcf_seqname(args->files->readers[0].header,arec), arec->pos+1,args->files->readers[0].fname, args->prev_pos_check+1); args->prev_pos_check = arec->pos; return; } int m = args->mbuf; args->nbuf += 2; hts_expand(bcf1_t*,args->nbuf,args->mbuf,args->buf); for (i=m; imbuf; i++) args->buf[i] = bcf_init1(); SWAP(bcf1_t*, args->files->readers[0].buffer[0], args->buf[args->nbuf-2]); SWAP(bcf1_t*, args->files->readers[1].buffer[0], args->buf[args->nbuf-1]); } static void concat(args_t *args) { int i; if ( args->phased_concat ) // phased concat { // keep only two open files at a time while ( args->ifname < args->nfnames ) { int new_file = 0; while ( args->files->nreaders < 2 && args->ifname < args->nfnames ) { if ( !bcf_sr_add_reader(args->files,args->fnames[args->ifname]) ) error("Failed to open %s: %s\n", args->fnames[args->ifname],bcf_sr_strerror(args->files->errnum)); new_file = 1; args->ifname++; if ( args->start_pos[args->ifname-1]==-1 ) break; // new chromosome, start with only one file open if ( args->ifname < args->nfnames && args->start_pos[args->ifname]==-1 ) break; // next file starts on a different chromosome } // is there a line from the previous run? Seek the newly opened reader to that position int seek_pos = -1; int seek_chr = -1; if ( bcf_sr_has_line(args->files,0) ) { bcf1_t *line = bcf_sr_get_line(args->files,0); bcf_sr_seek(args->files, bcf_seqname(args->files->readers[0].header,line), line->pos); seek_pos = line->pos; seek_chr = bcf_hdr_name2id(args->out_hdr, bcf_seqname(args->files->readers[0].header,line)); } else if ( new_file ) bcf_sr_seek(args->files,NULL,0); // set to start int nret; while ( (nret = bcf_sr_next_line(args->files)) ) { if ( !bcf_sr_has_line(args->files,0) ) // no input from the first reader { // We are assuming that there is a perfect overlap, sites which are not present in both files are dropped if ( ! bcf_sr_region_done(args->files,0) ) continue; phased_flush(args); bcf_sr_remove_reader(args->files, 0); } // Get a line to learn about current position for (i=0; ifiles->nreaders; i++) if ( bcf_sr_has_line(args->files,i) ) break; bcf1_t *line = bcf_sr_get_line(args->files,i); // This can happen after bcf_sr_seek: indel may start before the coordinate which we seek to. if ( seek_chr>=0 && seek_pos>line->pos && seek_chr==bcf_hdr_name2id(args->out_hdr, bcf_seqname(args->files->readers[i].header,line)) ) continue; seek_pos = seek_chr = -1; // Check if the position overlaps with the next, yet unopened, reader int must_seek = 0; while ( args->ifname < args->nfnames && args->start_pos[args->ifname]!=-1 && line->pos >= args->start_pos[args->ifname] ) { must_seek = 1; if ( !bcf_sr_add_reader(args->files,args->fnames[args->ifname]) ) error("Failed to open %s: %s\n", args->fnames[args->ifname],bcf_sr_strerror(args->files->errnum)); args->ifname++; } if ( must_seek ) { bcf_sr_seek(args->files, bcf_seqname(args->files->readers[i].header,line), line->pos); seek_pos = line->pos; seek_chr = bcf_hdr_name2id(args->out_hdr, bcf_seqname(args->files->readers[i].header,line)); continue; } // We are assuming that there is a perfect overlap, sites which are not present in both files are dropped if ( args->files->nreaders>1 && !bcf_sr_has_line(args->files,1) && !bcf_sr_region_done(args->files,1) ) continue; phased_push(args, bcf_sr_get_line(args->files,0), args->files->nreaders>1 ? bcf_sr_get_line(args->files,1) : NULL); } if ( args->files->nreaders ) { phased_flush(args); while ( args->files->nreaders ) bcf_sr_remove_reader(args->files, 0); } } } else if ( args->files ) // combining overlapping files, using synced reader { while ( bcf_sr_next_line(args->files) ) { for (i=0; ifiles->nreaders; i++) { bcf1_t *line = bcf_sr_get_line(args->files,i); if ( !line ) continue; bcf_translate(args->out_hdr, args->files->readers[i].header, line); bcf_write1(args->out_fh, args->out_hdr, line); if ( args->remove_dups ) break; } } } else // concatenating { kstring_t tmp = {0,0,0}; int prev_chr_id = -1, prev_pos; bcf1_t *line = bcf_init(); for (i=0; infnames; i++) { htsFile *fp = hts_open(args->fnames[i], "r"); if ( !fp ) error("Failed to open: %s\n", args->fnames[i]); bcf_hdr_t *hdr = bcf_hdr_read(fp); if ( !hdr ) error("Failed to parse header: %s\n", args->fnames[i]); if ( !fp->is_bin && args->output_type&FT_VCF ) { line->max_unpack = BCF_UN_STR; // if VCF is on both input and output, avoid VCF to BCF conversion while ( hts_getline(fp, KS_SEP_LINE, &fp->line) >=0 ) { char *str = fp->line.s; while ( *str && *str!='\t' ) str++; tmp.l = 0; kputsn(fp->line.s,str-fp->line.s,&tmp); int chr_id = bcf_hdr_name2id(args->out_hdr, tmp.s); if ( chr_id<0 ) error("The sequence \"%s\" not defined in the header: %s\n(Quick workaround: index the file.)\n", tmp.s, args->fnames[i]); if ( prev_chr_id!=chr_id ) { prev_pos = -1; if ( args->seen_seq[chr_id] ) error("\nThe chromosome block %s is not contiguous, consider running with -a.\n", tmp.s); } char *end; int pos = strtol(str+1,&end,10) - 1; if ( end==str+1 ) error("Could not parse line: %s\n", fp->line.s); if ( prev_pos > pos ) error("The chromosome block %s is not sorted, consider running with -a.\n", tmp.s); args->seen_seq[chr_id] = 1; prev_chr_id = chr_id; if ( vcf_write_line(args->out_fh, &fp->line)!=0 ) error("Failed to write %d bytes\n", fp->line.l); } } else { // BCF conversion is required line->max_unpack = 0; while ( bcf_read(fp, hdr, line)==0 ) { bcf_translate(args->out_hdr, hdr, line); if ( prev_chr_id!=line->rid ) { prev_pos = -1; if ( args->seen_seq[line->rid] ) error("\nThe chromosome block %s is not contiguous, consider running with -a.\n", bcf_seqname(args->out_hdr, line)); } if ( prev_pos > line->pos ) error("The chromosome block %s is not sorted, consider running with -a.\n", bcf_seqname(args->out_hdr, line)); args->seen_seq[line->rid] = 1; prev_chr_id = line->rid; if ( bcf_write(args->out_fh, args->out_hdr, line)!=0 ) error("Failed to write\n"); } } bcf_hdr_destroy(hdr); hts_close(fp); } bcf_destroy(line); free(tmp.s); } } static void usage(args_t *args) { fprintf(stderr, "\n"); fprintf(stderr, "About: Concatenate or combine VCF/BCF files. All source files must have the same sample\n"); fprintf(stderr, " columns appearing in the same order. Can be used, for example, to\n"); fprintf(stderr, " concatenate chromosome VCFs into one VCF, or combine a SNP VCF and an indel\n"); fprintf(stderr, " VCF into one. The input files must be sorted by chr and position. The files\n"); fprintf(stderr, " must be given in the correct order to produce sorted VCF on output unless\n"); fprintf(stderr, " the -a, --allow-overlaps option is specified.\n"); fprintf(stderr, "Usage: bcftools concat [options] [ [...]]\n"); fprintf(stderr, "\n"); fprintf(stderr, "Options:\n"); fprintf(stderr, " -a, --allow-overlaps First coordinate of the next file can precede last record of the current file.\n"); fprintf(stderr, " -D, --remove-duplicates Output only once records present in multiple files.\n"); fprintf(stderr, " -f, --file-list Read the list of files from a file.\n"); fprintf(stderr, " -l, --ligate Ligate phased VCFs by matching phase at overlapping haplotypes\n"); fprintf(stderr, " -q, --min-PQ Break phase set if phasing quality is lower than [30]\n"); fprintf(stderr, " -o, --output Write output to a file [standard output]\n"); fprintf(stderr, " -O, --output-type b: compressed BCF, u: uncompressed BCF, z: compressed VCF, v: uncompressed VCF [v]\n"); fprintf(stderr, " -r, --regions restrict to comma-separated list of regions\n"); fprintf(stderr, " -R, --regions-file restrict to regions listed in a file\n"); fprintf(stderr, "\n"); exit(1); } int main_vcfconcat(int argc, char *argv[]) { int c; args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->argc = argc; args->argv = argv; args->output_fname = "-"; args->output_type = FT_VCF; args->min_PQ = 30; static struct option loptions[] = { {"regions",1,0,'r'}, {"regions-file",1,0,'R'}, {"remove-duplicates",0,0,'D'}, {"allow-overlaps",0,0,'a'}, {"ligate",0,0,'l'}, {"output",1,0,'o'}, {"output-type",1,0,'O'}, {"file-list",1,0,'f'}, {"min-PQ",1,0,'q'}, {0,0,0,0} }; char *tmp; while ((c = getopt_long(argc, argv, "h:?o:O:f:alq:Dr:R:",loptions,NULL)) >= 0) { switch (c) { case 'r': args->regions_list = optarg; break; case 'R': args->regions_list = optarg; args->regions_is_file = 1; break; case 'D': args->remove_dups = 1; break; case 'q': args->min_PQ = strtol(optarg,&tmp,10); if ( *tmp ) error("Could not parse argument: --min-PQ %s\n", optarg); break; case 'a': args->allow_overlaps = 1; break; case 'l': args->phased_concat = 1; break; case 'f': args->file_list = optarg; break; case 'o': args->output_fname = optarg; break; case 'O': switch (optarg[0]) { case 'b': args->output_type = FT_BCF_GZ; break; case 'u': args->output_type = FT_BCF; break; case 'z': args->output_type = FT_VCF_GZ; break; case 'v': args->output_type = FT_VCF; break; default: error("The output type \"%s\" not recognised\n", optarg); }; break; case 'h': case '?': usage(args); break; default: error("Unknown argument: %s\n", optarg); } } while ( optindnfnames++; args->fnames = (char **)realloc(args->fnames,sizeof(char*)*args->nfnames); args->fnames[args->nfnames-1] = strdup(argv[optind]); optind++; } if ( args->allow_overlaps && args->phased_concat ) args->allow_overlaps = 0; if ( args->file_list ) { if ( args->nfnames ) error("Cannot combine -l with file names on command line.\n"); args->fnames = hts_readlines(args->file_list, &args->nfnames); if ( !args->fnames ) error("Could not read the file: %s\n", args->file_list); } if ( !args->nfnames ) usage(args); if ( args->remove_dups && !args->allow_overlaps ) error("The -D option is supported only with -a\n"); if ( args->regions_list && !args->allow_overlaps ) error("The -r/-R option is supported only with -a\n"); init_data(args); concat(args); destroy_data(args); free(args); return 0; } bcftools-1.2/vcfconvert.c000066400000000000000000001501631246371514100155240ustar00rootroot00000000000000/* vcfconvert.c -- convert between VCF/BCF and related formats. Copyright (C) 2013-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include #include #include #include #include #include "bcftools.h" #include "filter.h" #include "convert.h" #include "tsv2vcf.h" // Logic of the filters: include or exclude sites which match the filters? #define FLT_INCLUDE 1 #define FLT_EXCLUDE 2 typedef struct _args_t args_t; struct _args_t { faidx_t *ref; filter_t *filter; char *filter_str; int filter_logic; // include or exclude sites which match the filters? One of FLT_INCLUDE/FLT_EXCLUDE convert_t *convert; bcf_srs_t *files; bcf_hdr_t *header; void (*convert_func)(struct _args_t *); struct { int total, skipped, hom_rr, het_ra, hom_aa, het_aa, missing; } n; kstring_t str; int32_t *gts; float *flt; int rev_als, output_vcf_ids, hap2dip, output_chrom_first_col; int nsamples, *samples, sample_is_file, targets_is_file, regions_is_file, output_type; char **argv, *sample_list, *targets_list, *regions_list, *tag, *columns; char *outfname, *infname, *ref_fname; int argc; }; static void destroy_data(args_t *args) { if ( args->convert) convert_destroy(args->convert); if ( args->filter ) filter_destroy(args->filter); free(args->samples); if ( args->files ) bcf_sr_destroy(args->files); } static void open_vcf(args_t *args, const char *format_str) { args->files = bcf_sr_init(); if ( args->regions_list ) { if ( bcf_sr_set_regions(args->files, args->regions_list, args->regions_is_file)<0 ) error("Failed to read the regions: %s\n", args->regions_list); } if ( args->targets_list ) { if ( bcf_sr_set_targets(args->files, args->targets_list, args->targets_is_file, 0)<0 ) error("Failed to read the targets: %s\n", args->targets_list); } if ( !bcf_sr_add_reader(args->files, args->infname) ) error("Failed to open %s: %s\n", args->infname,bcf_sr_strerror(args->files->errnum)); if ( args->filter_str ) args->filter = filter_init(args->header, args->filter_str); args->header = args->files->readers[0].header; int i, nsamples = 0, *samples = NULL; if ( args->sample_list && strcmp("-",args->sample_list) ) { for (i=0; ifiles->nreaders; i++) { int ret = bcf_hdr_set_samples(args->files->readers[i].header,args->sample_list,args->sample_is_file); if ( ret<0 ) error("Error parsing the sample list\n"); else if ( ret>0 ) error("Sample name mismatch: sample #%d not found in the header\n", ret); } if ( args->sample_list[0]!='^' ) { // the sample ordering may be different if not negated int n; char **smpls = hts_readlist(args->sample_list, args->sample_is_file, &n); if ( !smpls ) error("Could not parse %s\n", args->sample_list); if ( n!=bcf_hdr_nsamples(args->files->readers[0].header) ) error("The number of samples does not match, perhaps some are present multiple times?\n"); nsamples = bcf_hdr_nsamples(args->files->readers[0].header); samples = (int*) malloc(sizeof(int)*nsamples); for (i=0; ifiles->readers[0].header, BCF_DT_SAMPLE,smpls[i]); free(smpls[i]); } free(smpls); } } if ( format_str ) args->convert = convert_init(args->header, samples, nsamples, format_str); free(samples); if ( args->filter_str ) args->filter = filter_init(args->header, args->filter_str); } static int tsv_setter_chrom_pos_ref_alt(tsv_t *tsv, bcf1_t *rec, void *usr) { args_t *args = (args_t*) usr; char tmp, *se = tsv->ss, *ss = tsv->ss; while ( se < tsv->se && *se!=':' ) se++; if ( *se!=':' ) error("Could not parse CHROM in CHROM:POS_REF_ALT id: %s\n", tsv->ss); tmp = *se; *se = 0; rec->rid = bcf_hdr_name2id(args->header,ss); if ( rec->rid<0 ) error("Could not determine sequence name or multiple sequences present: %s\n", tsv->ss); *se = tmp; // POS rec->pos = strtol(se+1,&ss,10); if ( ss==se+1 ) error("Could not parse POS in CHROM:POS_REF_ALT: %s\n", tsv->ss); rec->pos--; // REF,ALT args->str.l = 0; se = ++ss; while ( se < tsv->se && *se!='_' ) se++; if ( *se!='_' ) error("Could not parse REF in CHROM:POS_REF_ALT id: %s\n", tsv->ss); kputsn(ss,se-ss,&args->str); ss = ++se; while ( se < tsv->se && *se!=':' ) se++; if ( se < tsv->se && *se!=':' ) error("Could not parse ALT in CHROM:POS_REF_ALT id: %s\n", tsv->ss); kputc(',',&args->str); kputsn(ss,se-ss,&args->str); bcf_update_alleles_str(args->header, rec, args->str.s); return 0; } static int tsv_setter_verify_pos(tsv_t *tsv, bcf1_t *rec, void *usr) { char *se; int pos = strtol(tsv->ss,&se,10); if ( tsv->ss==se ) error("Could not parse POS: %s\n", tsv->ss); if ( rec->pos != pos-1 ) error("POS mismatch: %s\n", tsv->ss); return 0; } static int tsv_setter_verify_ref_alt(tsv_t *tsv, bcf1_t *rec, void *usr) { args_t *args = (args_t*) usr; args->rev_als = 0; char tmp = *tsv->se; *tsv->se = 0; if ( strcmp(tsv->ss,rec->d.allele[0]) ) { if ( strcmp(tsv->ss,rec->d.allele[1]) ) { *tsv->se = tmp; error("REF/ALT mismatch: [%s][%s]\n", tsv->ss,rec->d.allele[1]); } args->rev_als = 1; } *tsv->se = tmp; while ( *tsv->se && isspace(*tsv->se) ) tsv->se++; tsv->ss = tsv->se; while ( *tsv->se && !isspace(*tsv->se) ) tsv->se++; tmp = *tsv->se; *tsv->se = 0; if ( !args->rev_als && strcmp(tsv->ss,rec->d.allele[1]) ) { *tsv->se = tmp; error("REF/ALT mismatch: [%s][%s]\n", tsv->ss,rec->d.allele[1]); } else if ( args->rev_als && strcmp(tsv->ss,rec->d.allele[0]) ) { *tsv->se = tmp; error("REF/ALT mismatch: [%s][%s]\n", tsv->ss,rec->d.allele[0]); } *tsv->se = tmp; return 0; } static int tsv_setter_gt_gp(tsv_t *tsv, bcf1_t *rec, void *usr) { args_t *args = (args_t*) usr; int i, nsamples = bcf_hdr_nsamples(args->header); for (i=0; iss, &tsv->se); if ( tsv->ss==tsv->se ) { fprintf(stderr,"Could not parse first value of %d-th sample\n", i+1); return -1; } tsv->ss = tsv->se+1; ab = strtod(tsv->ss, &tsv->se); if ( tsv->ss==tsv->se ) { fprintf(stderr,"Could not parse second value of %d-th sample\n", i+1); return -1; } tsv->ss = tsv->se+1; bb = strtod(tsv->ss, &tsv->se); if ( tsv->ss==tsv->se ) { fprintf(stderr,"Could not parse third value of %d-th sample\n", i+1); return -1; } tsv->ss = tsv->se+1; if ( args->rev_als ) { float tmp = bb; bb = aa; aa = tmp; } args->flt[3*i+0] = aa; args->flt[3*i+1] = ab; args->flt[3*i+2] = bb; if ( aa >= ab ) { if ( aa >= bb ) args->gts[2*i+0] = args->gts[2*i+1] = bcf_gt_unphased(0); else args->gts[2*i+0] = args->gts[2*i+1] = bcf_gt_unphased(1); } else if ( ab >= bb ) { args->gts[2*i+0] = bcf_gt_unphased(0); args->gts[2*i+1] = bcf_gt_unphased(1); } else args->gts[2*i+0] = args->gts[2*i+1] = bcf_gt_unphased(1); } if ( *tsv->se ) error("Could not parse: %s\n", tsv->ss); if ( bcf_update_genotypes(args->header,rec,args->gts,nsamples*2) ) error("Could not update GT field\n"); if ( bcf_update_format_float(args->header,rec,"GP",args->flt,nsamples*3) ) error("Could not update GP field\n"); return 0; } static int tsv_setter_haps(tsv_t *tsv, bcf1_t *rec, void *usr) { args_t *args = (args_t*) usr; int i, nsamples = bcf_hdr_nsamples(args->header); int32_t a0, a1; if ( args->rev_als ) { a0 = bcf_gt_phased(1); a1 = bcf_gt_phased(0); } else { a0 = bcf_gt_phased(0); a1 = bcf_gt_phased(1); } for (i=0; iss + 4*i; if ( !ss[0] || !ss[1] || !ss[2] ) { fprintf(stderr,"Wrong number of fields at %d-th sample ([%c][%c][%c]). ",i+1,ss[0],ss[1],ss[2]); return -1; } if ( ss[0]=='0' ) args->gts[2*i] = a0; else if ( ss[0]=='1' ) args->gts[2*i] = a1; else { fprintf(stderr,"Could not parse: [%c][%s]\n", ss[0],tsv->ss); return -1; } if ( ss[2]=='0' ) args->gts[2*i+1] = a0; else if ( ss[2]=='1' ) args->gts[2*i+1] = a1; else { fprintf(stderr,"Could not parse: [%c][%s]\n", ss[2],tsv->ss); return -1; } } if ( tsv->ss[(nsamples-1)*4+3] ) { fprintf(stderr,"Wrong number of fields (%d-th column = [%c]). ", nsamples*2,tsv->ss[(nsamples-1)*4+1]); return -1; } if ( bcf_update_genotypes(args->header,rec,args->gts,nsamples*2) ) error("Could not update GT field\n"); return 0; } static void gensample_to_vcf(args_t *args) { /* * Inpute: IMPUTE2 output (indentation changed here for clarity): * * 20:62116619_C_T 20:62116619 62116619 C T 0.969 0.031 0 ... * --- 20:62116698_C_A 62116698 C A 1 0 0 ... * * Second column is expected in the form of CHROM:POS_REF_ALT. We use second * column because the first can be empty ("--") when filling sites from reference * panel. * * Output: VCF with filled GT,GP * */ kstring_t line = {0,0,0}; char *gen_fname = NULL, *sample_fname = NULL; sample_fname = strchr(args->infname,','); if ( !sample_fname ) { args->str.l = 0; ksprintf(&args->str,"%s.gen.gz", args->infname); gen_fname = strdup(args->str.s); args->str.l = 0; ksprintf(&args->str,"%s.samples", args->infname); sample_fname = strdup(args->str.s); } else { *sample_fname = 0; gen_fname = strdup(args->infname); sample_fname = strdup(sample_fname+1); } htsFile *gen_fh = hts_open(gen_fname, "r"); if ( !gen_fh ) error("Could not read: %s\n", gen_fname); if ( hts_getline(gen_fh, KS_SEP_LINE, &line) <= 0 ) error("Empty file: %s\n", gen_fname); // Find out the chromosome name, sample names, init and print the VCF header args->str.l = 0; char *ss, *se = line.s; while ( *se && !isspace(*se) ) se++; if ( !*se ) error("Could not parse %s: %s\n", gen_fname,line.s); ss = se+1; se = strchr(ss,':'); if ( !se ) error("Expected CHROM:POS_REF_ALT in second column of %s\n", gen_fname); kputsn(ss, se-ss, &args->str); tsv_t *tsv = tsv_init("-,CHROM_POS_REF_ALT,POS,REF_ALT,GT_GP"); tsv_register(tsv, "CHROM_POS_REF_ALT", tsv_setter_chrom_pos_ref_alt, args); tsv_register(tsv, "POS", tsv_setter_verify_pos, NULL); tsv_register(tsv, "REF_ALT", tsv_setter_verify_ref_alt, args); tsv_register(tsv, "GT_GP", tsv_setter_gt_gp, args); args->header = bcf_hdr_init("w"); bcf_hdr_append(args->header, "##FORMAT="); bcf_hdr_append(args->header, "##FORMAT="); bcf_hdr_printf(args->header, "##contig=", args->str.s,0x7fffffff); // MAX_CSI_COOR bcf_hdr_append_version(args->header, args->argc, args->argv, "bcftools_convert"); int i, nsamples; char **samples = hts_readlist(sample_fname, 1, &nsamples); for (i=2; iheader,samples[i]); } for (i=0; ioutfname,hts_bcf_wmode(args->output_type)); bcf_hdr_write(out_fh,args->header); bcf1_t *rec = bcf_init(); nsamples -= 2; args->gts = (int32_t *) malloc(sizeof(int32_t)*nsamples*2); args->flt = (float *) malloc(sizeof(float)*nsamples*3); do { bcf_clear(rec); args->n.total++; if ( !tsv_parse(tsv, rec, line.s) ) bcf_write(out_fh, args->header, rec); else error("Error occurred while parsing: %s\n", line.s); } while ( hts_getline(gen_fh, KS_SEP_LINE, &line)>0 ); if ( hts_close(out_fh) ) error("Close failed: %s\n", args->outfname); if ( hts_close(gen_fh) ) error("Close failed: %s\n", gen_fname); bcf_hdr_destroy(args->header); bcf_destroy(rec); free(sample_fname); free(gen_fname); free(args->str.s); free(line.s); free(args->gts); free(args->flt); tsv_destroy(tsv); fprintf(stderr,"Number of processed rows: \t%d\n", args->n.total); } static void haplegendsample_to_vcf(args_t *args) { /* * Convert from IMPUTE2 hap/legend/sample output files to VCF * * hap: * 0 1 0 1 1 1 * legend: * id position a0 a1 * 1:186946386_G_T 186946386 G T * sample: * QTL190044 * QTL190053 * * Output: VCF with filled GT */ kstring_t line = {0,0,0}; char *hap_fname = NULL, *leg_fname = NULL, *sample_fname = NULL; sample_fname = strchr(args->infname,','); if ( !sample_fname ) { args->str.l = 0; ksprintf(&args->str,"%s.hap.gz", args->infname); hap_fname = strdup(args->str.s); args->str.l = 0; ksprintf(&args->str,"%s.samples", args->infname); sample_fname = strdup(args->str.s); args->str.l = 0; ksprintf(&args->str,"%s.legend.gz", args->infname); leg_fname = strdup(args->str.s); } else { char *ss = sample_fname, *se = strchr(ss+1,','); if ( !se ) error("Could not parse hap/legend/sample file names: %s\n", args->infname); *ss = 0; *se = 0; hap_fname = strdup(args->infname); leg_fname = strdup(ss+1); sample_fname = strdup(se+1); } htsFile *hap_fh = hts_open(hap_fname, "r"); if ( !hap_fh ) error("Could not read: %s\n", hap_fname); htsFile *leg_fh = hts_open(leg_fname,"r"); if ( !leg_fh ) error("Could not read: %s\n", leg_fname); // Eat up first legend line, then determine chromosome name if ( hts_getline(leg_fh, KS_SEP_LINE, &line) <= 0 ) error("Empty file: %s\n", leg_fname); if ( hts_getline(leg_fh, KS_SEP_LINE, &line) <= 0 ) error("Empty file: %s\n", leg_fname); // Find out the chromosome name, sample names, init and print the VCF header args->str.l = 0; char *se = strchr(line.s,':'); if ( !se ) error("Expected CHROM:POS_REF_ALT in first column of %s\n", leg_fname); kputsn(line.s, se-line.s, &args->str); tsv_t *leg_tsv = tsv_init("CHROM_POS_REF_ALT,POS,REF_ALT"); tsv_register(leg_tsv, "CHROM_POS_REF_ALT", tsv_setter_chrom_pos_ref_alt, args); tsv_register(leg_tsv, "POS", tsv_setter_verify_pos, NULL); tsv_register(leg_tsv, "REF_ALT", tsv_setter_verify_ref_alt, args); tsv_t *hap_tsv = tsv_init("HAPS"); tsv_register(hap_tsv, "HAPS", tsv_setter_haps, args); args->header = bcf_hdr_init("w"); bcf_hdr_append(args->header, "##FORMAT="); bcf_hdr_printf(args->header, "##contig=", args->str.s,0x7fffffff); // MAX_CSI_COOR bcf_hdr_append_version(args->header, args->argc, args->argv, "bcftools_convert"); int i, nsamples; char **samples = hts_readlist(sample_fname, 1, &nsamples); for (i=0; iheader,samples[i]); } bcf_hdr_add_sample(args->header,NULL); for (i=0; ioutfname,hts_bcf_wmode(args->output_type)); bcf_hdr_write(out_fh,args->header); bcf1_t *rec = bcf_init(); args->gts = (int32_t *) malloc(sizeof(int32_t)*nsamples*2); while (1) { bcf_clear(rec); args->n.total++; if ( tsv_parse(leg_tsv, rec, line.s) ) error("Error occurred while parsing %s: %s\n", leg_fname,line.s); if ( hts_getline(hap_fh, KS_SEP_LINE, &line)<=0 ) error("Different number of records in %s and %s?\n", leg_fname,hap_fname); if ( tsv_parse(hap_tsv, rec, line.s) ) error("Error occurred while parsing %s: %s\n", hap_fname,line.s); bcf_write(out_fh, args->header, rec); if ( hts_getline(leg_fh, KS_SEP_LINE, &line)<=0 ) { if ( hts_getline(hap_fh, KS_SEP_LINE, &line)>0 ) error("Different number of records in %s and %s?\n", leg_fname,hap_fname); break; } } if ( hts_close(out_fh) ) error("Close failed: %s\n", args->outfname); if ( hts_close(hap_fh) ) error("Close failed: %s\n", hap_fname); if ( hts_close(leg_fh) ) error("Close failed: %s\n", leg_fname); bcf_hdr_destroy(args->header); bcf_destroy(rec); free(sample_fname); free(hap_fname); free(leg_fname); free(args->str.s); free(line.s); free(args->gts); tsv_destroy(hap_tsv); tsv_destroy(leg_tsv); fprintf(stderr,"Number of processed rows: \t%d\n", args->n.total); } static void hapsample_to_vcf(args_t *args) { /* * Input: SHAPEIT output * * 20:19995888_A_G 20:19995888 19995888 A G 0 0 0 0 ... * * First column is expected in the form of CHROM:POS_REF_ALT * * Output: VCF with filled GT * */ kstring_t line = {0,0,0}; char *hap_fname = NULL, *sample_fname = NULL; sample_fname = strchr(args->infname,','); if ( !sample_fname ) { args->str.l = 0; ksprintf(&args->str,"%s.hap.gz", args->infname); hap_fname = strdup(args->str.s); args->str.l = 0; ksprintf(&args->str,"%s.samples", args->infname); sample_fname = strdup(args->str.s); } else { *sample_fname = 0; hap_fname = strdup(args->infname); sample_fname = strdup(sample_fname+1); } htsFile *hap_fh = hts_open(hap_fname, "r"); if ( !hap_fh ) error("Could not read: %s\n", hap_fname); if ( hts_getline(hap_fh, KS_SEP_LINE, &line) <= 0 ) error("Empty file: %s\n", hap_fname); // Find out the chromosome name, sample names, init and print the VCF header args->str.l = 0; char *se = strchr(line.s,':'); if ( !se ) error("Expected CHROM:POS_REF_ALT in first column of %s\n", hap_fname); kputsn(line.s, se-line.s, &args->str); tsv_t *tsv = tsv_init("CHROM_POS_REF_ALT,-,POS,REF_ALT,HAPS"); tsv_register(tsv, "CHROM_POS_REF_ALT", tsv_setter_chrom_pos_ref_alt, args); tsv_register(tsv, "POS", tsv_setter_verify_pos, NULL); tsv_register(tsv, "REF_ALT", tsv_setter_verify_ref_alt, args); tsv_register(tsv, "HAPS", tsv_setter_haps, args); args->header = bcf_hdr_init("w"); bcf_hdr_append(args->header, "##FORMAT="); bcf_hdr_printf(args->header, "##contig=", args->str.s,0x7fffffff); // MAX_CSI_COOR bcf_hdr_append_version(args->header, args->argc, args->argv, "bcftools_convert"); int i, nsamples; char **samples = hts_readlist(sample_fname, 1, &nsamples); for (i=2; iheader,samples[i]); } bcf_hdr_add_sample(args->header,NULL); for (i=0; ioutfname,hts_bcf_wmode(args->output_type)); bcf_hdr_write(out_fh,args->header); bcf1_t *rec = bcf_init(); nsamples -= 2; args->gts = (int32_t *) malloc(sizeof(int32_t)*nsamples*2); do { bcf_clear(rec); args->n.total++; if ( !tsv_parse(tsv, rec, line.s) ) bcf_write(out_fh, args->header, rec); else error("Error occurred while parsing: %s\n", line.s); } while ( hts_getline(hap_fh, KS_SEP_LINE, &line)>0 ); if ( hts_close(out_fh) ) error("Close failed: %s\n", args->outfname); if ( hts_close(hap_fh) ) error("Close failed: %s\n", hap_fname); bcf_hdr_destroy(args->header); bcf_destroy(rec); free(sample_fname); free(hap_fname); free(args->str.s); free(line.s); free(args->gts); tsv_destroy(tsv); fprintf(stderr,"Number of processed rows: \t%d\n", args->n.total); } static void vcf_to_gensample(args_t *args) { kstring_t str = {0,0,0}; // insert chrom as first column if needed if(args->output_chrom_first_col) kputs("%CHROM ", &str); else kputs("%CHROM:%POS\\_%REF\\_%FIRST_ALT ", &str); // insert rsid as second column if needed if(args->output_vcf_ids) kputs("%ID ", &str); else kputs("%CHROM:%POS\\_%REF\\_%FIRST_ALT ", &str); kputs("%POS %REF %FIRST_ALT", &str); if ( !args->tag || !strcmp(args->tag,"GT") ) kputs("%_GT_TO_PROB3",&str); else if ( !strcmp(args->tag,"PL") ) kputs("%_PL_TO_PROB3",&str); else if ( !strcmp(args->tag,"GP") ) kputs("%_GP_TO_PROB3",&str); else error("todo: --tag %s\n", args->tag); kputs("\n", &str); open_vcf(args,str.s); int ret, gen_compressed = 1, sample_compressed = 0; char *gen_fname = NULL, *sample_fname = NULL; str.l = 0; kputs(args->outfname,&str); int n_files, i; char **files = hts_readlist(str.s, 0, &n_files); if ( n_files==1 ) { int l = str.l; kputs(".samples",&str); sample_fname = strdup(str.s); str.l = l; kputs(".gen.gz",&str); gen_fname = strdup(str.s); } else if ( n_files==2 ) { if (strlen(files[0]) && strcmp(files[0],".")!=0) gen_fname = strdup(files[0]); if (strlen(files[1]) && strcmp(files[1],".")!=0) sample_fname = strdup(files[1]); } else { error("Error parsing --gensample filenames: %s\n", args->outfname); } for (i=0; i3 && strcasecmp(".gz",sample_fname+strlen(sample_fname)-3)==0 ) sample_compressed = 0; if (gen_fname) fprintf(stderr, "Gen file: %s\n", gen_fname); if (sample_fname) fprintf(stderr, "Sample file: %s\n", sample_fname); // write samples file if (sample_fname) { int i; BGZF *sout = bgzf_open(sample_fname, sample_compressed ? "wg" : "wu"); str.l = 0; kputs("ID_1 ID_2 missing\n0 0 0\n", &str); ret = bgzf_write(sout, str.s, str.l); if ( ret != str.l ) error("Error writing %s: %s\n", sample_fname, strerror(errno)); for (i=0; iheader); i++) { str.l = 0; ksprintf(&str, "%s %s 0\n", args->header->samples[i],args->header->samples[i]); ret = bgzf_write(sout, str.s, str.l); if ( ret != str.l ) error("Error writing %s: %s\n", sample_fname, strerror(errno)); } if ( bgzf_close(sout)!=0 ) error("Error closing %s: %s\n", sample_fname, strerror(errno)); free(sample_fname); } if (!gen_fname) { if ( str.m ) free(str.s); return; } int prev_rid = -1, prev_pos = -1; int no_alt = 0, non_biallelic = 0, filtered = 0, ndup = 0; BGZF *gout = bgzf_open(gen_fname, gen_compressed ? "wg" : "wu"); while ( bcf_sr_next_line(args->files) ) { bcf1_t *line = bcf_sr_get_line(args->files,0); if ( args->filter ) { int pass = filter_test(args->filter, line, NULL); if ( args->filter_logic & FLT_EXCLUDE ) pass = pass ? 0 : 1; if ( !pass ) { filtered++; continue; } } // ALT allele is required if ( line->n_allele<2 ) { no_alt++; continue; } // biallelic required if ( line->n_allele>2 ) { if (!non_biallelic) fprintf(stderr, "Warning: non-biallelic records are skipped. Consider splitting multi-allelic records into biallelic records using 'bcftools norm -m-'.\n"); non_biallelic++; continue; } // skip duplicate lines, or otherwise shapeit complains if ( prev_rid==line->rid && prev_pos==line->pos ) { ndup++; continue; } prev_rid = line->rid; prev_pos = line->pos; str.l = 0; convert_line(args->convert, line, &str); if ( str.l ) { int ret = bgzf_write(gout, str.s, str.l); if ( ret!= str.l ) error("Error writing %s: %s\n", gen_fname,strerror(errno)); } } fprintf(stderr, "%d records skipped: %d/%d/%d/%d no-ALT/non-biallelic/filtered/duplicated\n", no_alt+non_biallelic+filtered+ndup, no_alt, non_biallelic, filtered, ndup); if ( str.m ) free(str.s); if ( bgzf_close(gout)!=0 ) error("Error closing %s: %s\n", gen_fname,strerror(errno)); free(gen_fname); } static void vcf_to_haplegendsample(args_t *args) { kstring_t str = {0,0,0}; if ( args->hap2dip ) kputs("%_GT_TO_HAP2\n", &str); else kputs("%_GT_TO_HAP\n", &str); open_vcf(args,str.s); int ret, hap_compressed = 1, legend_compressed = 1, sample_compressed = 0; char *hap_fname = NULL, *legend_fname = NULL, *sample_fname = NULL; str.l = 0; kputs(args->outfname,&str); int n_files, i; char **files = hts_readlist(str.s, 0, &n_files); if ( n_files==1 ) { int l = str.l; kputs(".samples",&str); sample_fname = strdup(str.s); str.l = l; kputs(".legend.gz",&str); legend_fname = strdup(str.s); str.l = l; kputs(".hap.gz",&str); hap_fname = strdup(str.s); } else if ( n_files==3 ) { if (strlen(files[0]) && strcmp(files[0],".")!=0) hap_fname = strdup(files[0]); if (strlen(files[1]) && strcmp(files[1],".")!=0) legend_fname = strdup(files[1]); if (strlen(files[2]) && strcmp(files[2],".")!=0) sample_fname = strdup(files[2]); } else { error("Error parsing --hapslegendsample filenames: %s\n", args->outfname); } for (i=0; i3 && strcasecmp(".gz",sample_fname+strlen(sample_fname)-3)==0 ) sample_compressed = 0; if (hap_fname) fprintf(stderr, "Haps file: %s\n", hap_fname); if (legend_fname) fprintf(stderr, "Legend file: %s\n", legend_fname); if (sample_fname) fprintf(stderr, "Sample file: %s\n", sample_fname); // write samples file if (sample_fname) { int i; BGZF *sout = bgzf_open(sample_fname, sample_compressed ? "wg" : "wu"); str.l = 0; kputs("sample population group sex\n", &str); ret = bgzf_write(sout, str.s, str.l); if ( ret != str.l ) error("Error writing %s: %s\n", sample_fname, strerror(errno)); for (i=0; iheader); i++) { str.l = 0; ksprintf(&str, "%s %s %s 2\n", args->header->samples[i], args->header->samples[i], args->header->samples[i]); ret = bgzf_write(sout, str.s, str.l); if ( ret != str.l ) error("Error writing %s: %s\n", sample_fname, strerror(errno)); } if ( bgzf_close(sout)!=0 ) error("Error closing %s: %s\n", sample_fname, strerror(errno)); free(sample_fname); } if (!hap_fname && !legend_fname) { if ( str.m ) free(str.s); return; } // open haps and legend outputs BGZF *hout = hap_fname ? bgzf_open(hap_fname, hap_compressed ? "wg" : "wu") : NULL; BGZF *lout = legend_fname ? bgzf_open(legend_fname, legend_compressed ? "wg" : "wu") : NULL; if (legend_fname) { str.l = 0; kputs("id position a0 a1\n", &str); ret = bgzf_write(lout, str.s, str.l); if ( ret != str.l ) error("Error writing %s: %s\n", legend_fname, strerror(errno)); } int no_alt = 0, non_biallelic = 0, filtered = 0; while ( bcf_sr_next_line(args->files) ) { bcf1_t *line = bcf_sr_get_line(args->files,0); if ( args->filter ) { int pass = filter_test(args->filter, line, NULL); if ( args->filter_logic & FLT_EXCLUDE ) pass = pass ? 0 : 1; if ( !pass ) { filtered++; continue; } } // ALT allele is required if ( line->n_allele<2 ) { no_alt++; continue; } // biallelic required if ( line->n_allele>2 ) { if (!non_biallelic) fprintf(stderr, "Warning: non-biallelic records are skipped. Consider splitting multi-allelic records into biallelic records using 'bcftools norm -m-'.\n"); non_biallelic++; continue; } str.l = 0; convert_line(args->convert, line, &str); if ( !str.l ) continue; // write haps file if (hap_fname) { ret = bgzf_write(hout, str.s, str.l); // write hap file if ( ret != str.l ) error("Error writing %s: %s\n", hap_fname, strerror(errno)); } if (legend_fname) { str.l = 0; if ( args->output_vcf_ids && (line->d.id[0]!='.' || line->d.id[1]!=0) ) ksprintf(&str, "%s %d %s %s\n", line->d.id, line->pos+1, line->d.allele[0], line->d.allele[1]); else ksprintf(&str, "%s:%d_%s_%s %d %s %s\n", bcf_seqname(args->header, line), line->pos+1, line->d.allele[0], line->d.allele[1], line->pos+1, line->d.allele[0], line->d.allele[1]); // write legend file ret = bgzf_write(lout, str.s, str.l); if ( ret != str.l ) error("Error writing %s: %s\n", legend_fname, strerror(errno)); } } fprintf(stderr, "%d records skipped: %d/%d/%d no-ALT/non-biallelic/filtered\n", no_alt+non_biallelic+filtered, no_alt, non_biallelic, filtered); if ( str.m ) free(str.s); if ( hout && bgzf_close(hout)!=0 ) error("Error closing %s: %s\n", hap_fname, strerror(errno)); if ( lout && bgzf_close(lout)!=0 ) error("Error closing %s: %s\n", legend_fname, strerror(errno)); if (hap_fname) free(hap_fname); if (legend_fname) free(legend_fname); } static void vcf_to_hapsample(args_t *args) { /* * WTCCC style haplotypes file * see https://mathgen.stats.ox.ac.uk/genetics_software/shapeit/shapeit.html#hapsample * * These are essentially the haplotypes from the impute2 format with some * legend info tacked on to the first 5 columns * */ kstring_t str = {0,0,0}; // print ID instead of CHROM:POS_REF_ALT1 if ( args->output_vcf_ids ) kputs("%CHROM %ID %POS %REF %FIRST_ALT ", &str); else kputs("%CHROM %CHROM:%POS\\_%REF\\_%FIRST_ALT %POS %REF %FIRST_ALT ", &str); if ( args->hap2dip ) kputs("%_GT_TO_HAP2\n", &str); else kputs("%_GT_TO_HAP\n", &str); open_vcf(args,str.s); int ret, hap_compressed = 1, sample_compressed = 0; char *hap_fname = NULL, *sample_fname = NULL; str.l = 0; kputs(args->outfname,&str); int n_files, i; char **files = hts_readlist(str.s, 0, &n_files); if ( n_files==1 ) { int l = str.l; kputs(".sample",&str); sample_fname = strdup(str.s); str.l = l; kputs(".hap.gz",&str); hap_fname = strdup(str.s); } else if ( n_files==2 ) { if (strlen(files[0]) && strcmp(files[0],".")!=0) hap_fname = strdup(files[0]); if (strlen(files[1]) && strcmp(files[1],".")!=0) sample_fname = strdup(files[1]); } else { error("Error parsing --hapsample filenames: %s\n", args->outfname); } for (i=0; i3 && strcasecmp(".gz",sample_fname+strlen(sample_fname)-3)==0 ) sample_compressed = 0; if (hap_fname) fprintf(stderr, "Haps file: %s\n", hap_fname); if (sample_fname) fprintf(stderr, "Sample file: %s\n", sample_fname); // write samples file if (sample_fname) { int i; BGZF *sout = bgzf_open(sample_fname, sample_compressed ? "wg" : "wu"); str.l = 0; kputs("ID_1 ID_2 missing\n0 0 0\n", &str); ret = bgzf_write(sout, str.s, str.l); if ( ret != str.l ) error("Error writing %s: %s\n", sample_fname, strerror(errno)); for (i=0; iheader); i++) { str.l = 0; ksprintf(&str, "%s %s 0\n", args->header->samples[i], args->header->samples[i]); ret = bgzf_write(sout, str.s, str.l); if ( ret != str.l ) error("Error writing %s: %s\n", sample_fname, strerror(errno)); } if ( bgzf_close(sout)!=0 ) error("Error closing %s: %s\n", sample_fname, strerror(errno)); free(sample_fname); } if (!hap_fname) { if ( str.m ) free(str.s); return; } // open haps output BGZF *hout = hap_fname ? bgzf_open(hap_fname, hap_compressed ? "wg" : "wu") : NULL; int no_alt = 0, non_biallelic = 0, filtered = 0; while ( bcf_sr_next_line(args->files) ) { bcf1_t *line = bcf_sr_get_line(args->files,0); if ( args->filter ) { int pass = filter_test(args->filter, line, NULL); if ( args->filter_logic & FLT_EXCLUDE ) pass = pass ? 0 : 1; if ( !pass ) { filtered++; continue; } } // ALT allele is required if ( line->n_allele<2 ) { no_alt++; continue; } // biallelic required if ( line->n_allele>2 ) { if (!non_biallelic) fprintf(stderr, "Warning: non-biallelic records are skipped. Consider splitting multi-allelic records into biallelic records using 'bcftools norm -m-'.\n"); non_biallelic++; continue; } str.l = 0; convert_line(args->convert, line, &str); if ( !str.l ) continue; // write haps file if (hap_fname) { ret = bgzf_write(hout, str.s, str.l); // write hap file if ( ret != str.l ) error("Error writing %s: %s\n", hap_fname, strerror(errno)); } } fprintf(stderr, "%d records skipped: %d/%d/%d no-ALT/non-biallelic/filtered\n", no_alt+non_biallelic+filtered, no_alt, non_biallelic, filtered); if ( str.m ) free(str.s); if ( hout && bgzf_close(hout)!=0 ) error("Error closing %s: %s\n", hap_fname, strerror(errno)); if (hap_fname) free(hap_fname); } static void bcf_hdr_set_chrs(bcf_hdr_t *hdr, faidx_t *fai) { int i, n = faidx_nseq(fai); for (i=0; i", seq,len); } } static inline int acgt_to_5(char base) { if ( base=='A' ) return 0; if ( base=='C' ) return 1; if ( base=='G' ) return 2; if ( base=='T' ) return 3; return 4; } static inline int tsv_setter_aa1(args_t *args, char *ss, char *se, int alleles[], int *nals, int ref, int32_t *gts) { if ( se - ss > 2 ) return -1; // currently only SNPs if ( ss[0]=='-' ) { // missing GT gts[0] = bcf_gt_missing; gts[1] = bcf_int32_vector_end; args->n.missing++; return 0; } if ( ss[0]=='I' ) return -2; // skip insertions/deletions for now if ( ss[0]=='D' ) return -2; int a0 = acgt_to_5(toupper(ss[0])); int a1 = ss[1] ? acgt_to_5(toupper(ss[1])) : a0; if ( alleles[a0]<0 ) alleles[a0] = (*nals)++; if ( alleles[a1]<0 ) alleles[a1] = (*nals)++; gts[0] = bcf_gt_unphased(alleles[a0]); gts[1] = ss[1] ? bcf_gt_unphased(alleles[a1]) : bcf_int32_vector_end; if ( ref==a0 && ref==a1 ) args->n.hom_rr++; // hom ref: RR else if ( ref==a0 ) args->n.het_ra++; // het: RA else if ( ref==a1 ) args->n.het_ra++; // het: AR else if ( a0==a1 ) args->n.hom_aa++; // hom-alt: AA else args->n.het_aa++; // non-ref het: AA return 0; } static int tsv_setter_aa(tsv_t *tsv, bcf1_t *rec, void *usr) { args_t *args = (args_t*) usr; int len; char *ref = faidx_fetch_seq(args->ref, (char*)bcf_hdr_id2name(args->header,rec->rid), rec->pos, rec->pos, &len); if ( !ref ) error("faidx_fetch_seq failed at %s:%d\n", bcf_hdr_id2name(args->header,rec->rid), rec->pos+1); int nals = 1, alleles[5] = { -1, -1, -1, -1, -1 }; // a,c,g,t,n ref[0] = toupper(ref[0]); int iref = acgt_to_5(ref[0]); alleles[iref] = 0; rec->n_sample = bcf_hdr_nsamples(args->header); int i, ret; for (i=0; in_sample; i++) { if ( i>0 ) { ret = tsv_next(tsv); if ( ret==-1 ) error("Too few columns for %d samples at %s:%d\n", rec->n_sample,bcf_hdr_id2name(args->header,rec->rid), rec->pos+1); } ret = tsv_setter_aa1(args, tsv->ss, tsv->se, alleles, &nals, iref, args->gts+i*2); if ( ret==-1 ) error("Error parsing the site %s:%d, expected two characters\n", bcf_hdr_id2name(args->header,rec->rid), rec->pos+1); if ( ret==-2 ) { // something else than a SNP free(ref); return -1; } } args->str.l = 0; kputc(ref[0], &args->str); for (i=0; i<5; i++) { if ( alleles[i]>0 ) { kputc(',', &args->str); kputc("ACGTN"[i], &args->str); } } bcf_update_alleles_str(args->header, rec, args->str.s); if ( bcf_update_genotypes(args->header,rec,args->gts,rec->n_sample*2) ) error("Could not update the GT field\n"); free(ref); return 0; } static void tsv_to_vcf(args_t *args) { if ( !args->ref_fname ) error("Missing the --ref option\n"); if ( !args->sample_list ) error("Missing the --samples option\n"); args->ref = fai_load(args->ref_fname); if ( !args->ref ) error("Could not load the reference %s\n", args->ref_fname); args->header = bcf_hdr_init("w"); bcf_hdr_set_chrs(args->header, args->ref); bcf_hdr_append(args->header, "##FORMAT="); bcf_hdr_append_version(args->header, args->argc, args->argv, "bcftools_convert"); int i, n; char **smpls = hts_readlist(args->sample_list, args->sample_is_file, &n); if ( !smpls ) error("Could not parse %s\n", args->sample_list); for (i=0; iheader, smpls[i]); free(smpls[i]); } free(smpls); bcf_hdr_add_sample(args->header, NULL); args->gts = (int32_t *) malloc(sizeof(int32_t)*n*2); htsFile *out_fh = hts_open(args->outfname,hts_bcf_wmode(args->output_type)); bcf_hdr_write(out_fh,args->header); tsv_t *tsv = tsv_init(args->columns ? args->columns : "ID,CHROM,POS,AA"); if ( tsv_register(tsv, "CHROM", tsv_setter_chrom, args->header) < 0 ) error("Expected CHROM column\n"); if ( tsv_register(tsv, "POS", tsv_setter_pos, NULL) < 0 ) error("Expected POS column\n"); if ( tsv_register(tsv, "ID", tsv_setter_id, args->header) < 0 && !args->columns ) error("Expected ID column\n"); if ( tsv_register(tsv, "AA", tsv_setter_aa, args) < 0 ) error("Expected AA column\n"); bcf1_t *rec = bcf_init(); bcf_float_set_missing(rec->qual); kstring_t line = {0,0,0}; htsFile *in_fh = hts_open(args->infname, "r"); if ( !in_fh ) error("Could not read: %s\n", args->infname); while ( hts_getline(in_fh, KS_SEP_LINE, &line) > 0 ) { if ( line.s[0]=='#' ) continue; // skip comments bcf_clear(rec); args->n.total++; if ( !tsv_parse(tsv, rec, line.s) ) bcf_write(out_fh, args->header, rec); else args->n.skipped++; } if ( hts_close(in_fh) ) error("Close failed: %s\n", args->infname); free(line.s); fai_destroy(args->ref); bcf_hdr_destroy(args->header); hts_close(out_fh); tsv_destroy(tsv); bcf_destroy(rec); free(args->str.s); free(args->gts); fprintf(stderr,"Rows total: \t%d\n", args->n.total); fprintf(stderr,"Rows skipped: \t%d\n", args->n.skipped); fprintf(stderr,"Missing GTs: \t%d\n", args->n.missing); fprintf(stderr,"Hom RR: \t%d\n", args->n.hom_rr); fprintf(stderr,"Het RA: \t%d\n", args->n.het_ra); fprintf(stderr,"Hom AA: \t%d\n", args->n.hom_aa); fprintf(stderr,"Het AA: \t%d\n", args->n.het_aa); } static void vcf_to_vcf(args_t *args) { open_vcf(args,NULL); htsFile *out_fh = hts_open(args->outfname,hts_bcf_wmode(args->output_type)); if ( !out_fh ) error("Failed to open: %s\n", args->outfname); bcf_hdr_t *hdr = bcf_sr_get_header(args->files,0); bcf_hdr_write(out_fh,hdr); while ( bcf_sr_next_line(args->files) ) { bcf1_t *line = bcf_sr_get_line(args->files,0); if ( args->filter ) { int pass = filter_test(args->filter, line, NULL); if ( args->filter_logic & FLT_EXCLUDE ) pass = pass ? 0 : 1; if ( !pass ) continue; } bcf_write(out_fh,hdr,line); } hts_close(out_fh); } static void gvcf_to_vcf(args_t *args) { open_vcf(args,NULL); htsFile *out_fh = hts_open(args->outfname,hts_bcf_wmode(args->output_type)); if ( !out_fh ) error("Failed to open: %s\n", args->outfname); bcf_hdr_t *hdr = bcf_sr_get_header(args->files,0); bcf_hdr_append_version(hdr, args->argc, args->argv, "bcftools_convert"); bcf_hdr_write(out_fh,hdr); int32_t *itmp = NULL, nitmp = 0; while ( bcf_sr_next_line(args->files) ) { bcf1_t *line = bcf_sr_get_line(args->files,0); if ( args->filter ) { int pass = filter_test(args->filter, line, NULL); if ( args->filter_logic & FLT_EXCLUDE ) pass = pass ? 0 : 1; if ( !pass ) continue; } if ( line->n_allele!=1 || !bcf_has_filter(hdr,line,"PASS") ) { // Assuming that only ALT=. sites can be blocks and skipping sites which don't PASS bcf_write(out_fh,hdr,line); continue; } int nend = bcf_get_info_int32(hdr,line,"END",&itmp,&nitmp); if ( nend!=1 ) { // No END lineord bcf_write(out_fh,hdr,line); continue; } bcf_update_info_int32(hdr,line,"END",NULL,0); int pos; for (pos=line->pos; pos<=itmp[0]; pos++) { line->pos = pos; bcf_write(out_fh,hdr,line); } } free(itmp); hts_close(out_fh); } static void usage(void) { fprintf(stderr, "\n"); fprintf(stderr, "About: Converts VCF/BCF to other formats and back. See man page for file\n"); fprintf(stderr, " formats details. When specifying output files explicitly instead\n"); fprintf(stderr, " of with , one can use '-' for stdout and '.' to suppress.\n"); fprintf(stderr, "Usage: bcftools convert [OPTIONS] \n"); fprintf(stderr, "\n"); fprintf(stderr, "VCF input options:\n"); fprintf(stderr, " -e, --exclude exclude sites for which the expression is true\n"); fprintf(stderr, " -i, --include select sites for which the expression is true\n"); fprintf(stderr, " -r, --regions restrict to comma-separated list of regions\n"); fprintf(stderr, " -R, --regions-file restrict to regions listed in a file\n"); fprintf(stderr, " -s, --samples list of samples to include\n"); fprintf(stderr, " -S, --samples-file file of samples to include\n"); fprintf(stderr, " -t, --targets similar to -r but streams rather than index-jumps\n"); fprintf(stderr, " -T, --targets-file similar to -R but streams rather than index-jumps\n"); fprintf(stderr, "\n"); fprintf(stderr, "VCF output options:\n"); fprintf(stderr, " -o, --output output file name [stdout]\n"); fprintf(stderr, " -O, --output-type b: compressed BCF, u: uncompressed BCF, z: compressed VCF, v: uncompressed VCF [v]\n"); fprintf(stderr, "\n"); fprintf(stderr, "GEN/SAMPLE conversion (input/output from IMPUTE2):\n"); fprintf(stderr, " -G, --gensample2vcf <...> |,\n"); fprintf(stderr, " -g, --gensample <...> |,\n"); fprintf(stderr, " --tag tag to take values for .gen file: GT,PL,GL,GP [GT]\n"); fprintf(stderr, " --chrom output chromosome in first column instead of CHROM:POS_REF_ALT\n"); fprintf(stderr, " --vcf-ids output VCF IDs in second column instead of CHROM:POS_REF_ALT\n"); fprintf(stderr, "\n"); fprintf(stderr, "gVCF conversion:\n"); fprintf(stderr, " --gvcf2vcf \n"); fprintf(stderr, "\n"); fprintf(stderr, "HAP/SAMPLE conversion (output from SHAPEIT):\n"); fprintf(stderr, " --hapsample2vcf <...> |,\n"); fprintf(stderr, " --hapsample <...> |,\n"); fprintf(stderr, " --haploid2diploid convert haploid genotypes to diploid homozygotes\n"); fprintf(stderr, " --vcf-ids output VCF IDs instead of CHROM:POS_REF_ALT\n"); fprintf(stderr, "\n"); fprintf(stderr, "HAP/LEGEND/SAMPLE conversion:\n"); fprintf(stderr, " -H, --haplegendsample2vcf <...> |,,\n"); fprintf(stderr, " -h, --haplegendsample <...> |,,\n"); fprintf(stderr, " --haploid2diploid convert haploid genotypes to diploid homozygotes\n"); fprintf(stderr, " --vcf-ids output VCF IDs instead of CHROM:POS_REF_ALT\n"); fprintf(stderr, "\n"); fprintf(stderr, "TSV conversion:\n"); fprintf(stderr, " --tsv2vcf \n"); fprintf(stderr, " -c, --columns columns of the input tsv file [ID,CHROM,POS,AA]\n"); fprintf(stderr, " -f, --fasta-ref reference sequence in fasta format\n"); fprintf(stderr, " -s, --samples list of sample names\n"); fprintf(stderr, " -S, --samples-file file of sample names\n"); fprintf(stderr, "\n"); // fprintf(stderr, "PLINK options:\n"); // fprintf(stderr, " -p, --plink |,,|,,|,\n"); // fprintf(stderr, " --tped make tped file instead\n"); // fprintf(stderr, " --bin make binary bed/fam/bim files\n"); // fprintf(stderr, "\n"); // fprintf(stderr, "PBWT options:\n"); // fprintf(stderr, " -b, --pbwt or ,,,\n"); // fprintf(stderr, "\n"); exit(1); } int main_vcfconvert(int argc, char *argv[]) { int c; args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->argc = argc; args->argv = argv; args->outfname = "-"; args->output_type = FT_VCF; static struct option loptions[] = { {"include",required_argument,NULL,'i'}, {"exclude",required_argument,NULL,'e'}, {"output",required_argument,NULL,'o'}, {"output-type",required_argument,NULL,'O'}, {"regions",required_argument,NULL,'r'}, {"regions-file",required_argument,NULL,'R'}, {"targets",required_argument,NULL,'t'}, {"targets-file",required_argument,NULL,'T'}, {"samples",required_argument,NULL,'s'}, {"samples-file",required_argument,NULL,'S'}, {"gensample",required_argument,NULL,'g'}, {"gensample2vcf",required_argument,NULL,'G'}, {"tag",required_argument,NULL,1}, {"chrom",no_argument,NULL,8}, {"tsv2vcf",required_argument,NULL,2}, {"hapsample",required_argument,NULL,7}, {"hapsample2vcf",required_argument,NULL,3}, {"vcf-ids",no_argument,NULL,4}, {"haploid2diploid",no_argument,NULL,5}, {"gvcf2vcf",no_argument,NULL,6}, {"haplegendsample",required_argument,NULL,'h'}, {"haplegendsample2vcf",required_argument,NULL,'H'}, {"columns",required_argument,NULL,'c'}, {"fasta-ref",required_argument,NULL,'f'}, {0,0,0,0} }; while ((c = getopt_long(argc, argv, "?h:r:R:s:S:t:T:i:e:g:G:o:O:c:f:H:",loptions,NULL)) >= 0) { switch (c) { case 'e': args->filter_str = optarg; args->filter_logic |= FLT_EXCLUDE; break; case 'i': args->filter_str = optarg; args->filter_logic |= FLT_INCLUDE; break; case 'r': args->regions_list = optarg; break; case 'R': args->regions_list = optarg; args->regions_is_file = 1; break; case 't': args->targets_list = optarg; break; case 'T': args->targets_list = optarg; args->targets_is_file = 1; break; case 's': args->sample_list = optarg; break; case 'S': args->sample_list = optarg; args->sample_is_file = 1; break; case 'g': args->convert_func = vcf_to_gensample; args->outfname = optarg; break; case 'G': args->convert_func = gensample_to_vcf; args->infname = optarg; break; case 1 : args->tag = optarg; break; case 2 : args->convert_func = tsv_to_vcf; args->infname = optarg; break; case 3 : args->convert_func = hapsample_to_vcf; args->infname = optarg; break; case 4 : args->output_vcf_ids = 1; break; case 5 : args->hap2dip = 1; break; case 6 : args->convert_func = gvcf_to_vcf; break; case 7 : args->convert_func = vcf_to_hapsample; args->outfname = optarg; break; case 8 : args->output_chrom_first_col = 1; break; case 'H': args->convert_func = haplegendsample_to_vcf; args->infname = optarg; break; case 'f': args->ref_fname = optarg; break; case 'c': args->columns = optarg; break; case 'o': args->outfname = optarg; break; case 'O': switch (optarg[0]) { case 'b': args->output_type = FT_BCF_GZ; break; case 'u': args->output_type = FT_BCF; break; case 'z': args->output_type = FT_VCF_GZ; break; case 'v': args->output_type = FT_VCF; break; default: error("The output type \"%s\" not recognised\n", optarg); } break; case 'h': args->convert_func = vcf_to_haplegendsample; args->outfname = optarg; break; case '?': usage(); default: error("Unknown argument: %s\n", optarg); } } if ( !args->infname ) { if ( optind>=argc ) { if ( !isatty(fileno((FILE *)stdin)) ) args->infname = "-"; } else args->infname = argv[optind]; } if ( !args->infname ) usage(); if ( args->convert_func ) args->convert_func(args); else vcf_to_vcf(args); destroy_data(args); free(args); return 0; } bcftools-1.2/vcffilter.c000066400000000000000000000532461246371514100153350ustar00rootroot00000000000000/* vcffilter.c -- Apply fixed-threshold filters. Copyright (C) 2013-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include #include #include #include "bcftools.h" #include "filter.h" #include "rbuf.h" // Logic of the filters: include or exclude sites which match the filters? #define FLT_INCLUDE 1 #define FLT_EXCLUDE 2 // FILTER columns annotation: replace or add to existing FILTERs; set FILTER to PASS at good sites? #define ANNOT_ADD 1 #define ANNOT_RESET 2 // Set genotypes of filtered samples #define SET_GTS_MISSING 1 #define SET_GTS_REF 2 typedef struct _args_t { filter_t *filter; char *filter_str; int filter_logic; // include or exclude sites which match the filters? One of FLT_INCLUDE/FLT_EXCLUDE const uint8_t *smpl_pass; int set_gts; char *soft_filter; // drop failed sites or annotate FILTER column? int annot_mode; // add to existing FILTER annotation or replace? Otherwise reset FILTER to PASS or leave as it is? int flt_fail, flt_pass; // BCF ids of fail and pass filters int snp_gap, indel_gap, IndelGap_id, SnpGap_id; int32_t ntmpi, *tmpi, ntmp_ac, *tmp_ac; rbuf_t rbuf; bcf1_t **rbuf_lines; bcf_srs_t *files; bcf_hdr_t *hdr; htsFile *out_fh; int output_type; char **argv, *output_fname, *targets_list, *regions_list; int argc; } args_t; static void init_data(args_t *args) { args->out_fh = hts_open(args->output_fname,hts_bcf_wmode(args->output_type)); if ( args->out_fh == NULL ) error("Can't write to \"%s\": %s\n", args->output_fname, strerror(errno)); args->hdr = args->files->readers[0].header; args->flt_pass = bcf_hdr_id2int(args->hdr,BCF_DT_ID,"PASS"); assert( !args->flt_pass ); // sanity check: required by BCF spec // -i or -e: append FILTER line if ( args->soft_filter && args->filter_logic ) { kstring_t flt_name = {0,0,0}; if ( strcmp(args->soft_filter,"+") ) kputs(args->soft_filter, &flt_name); else { // Make up a filter name int i = 0, id = -1; do { ksprintf(&flt_name,"Filter%d", ++i); id = bcf_hdr_id2int(args->hdr,BCF_DT_ID,flt_name.s); } while ( bcf_hdr_idinfo_exists(args->hdr,BCF_HL_FLT,id) ); } // escape quotes kstring_t tmp = {0,0,0}; char *t = args->filter_str; while ( *t ) { if ( *t=='"' ) kputc('\\',&tmp); kputc(*t,&tmp); t++; } int ret = bcf_hdr_printf(args->hdr, "##FILTER=", flt_name.s,args->filter_logic & FLT_INCLUDE ? "not true" : "true", tmp.s); if ( ret!=0 ) error("Failed to append header line: ##FILTER=\n", flt_name.s,args->filter_logic & FLT_INCLUDE ? "not true" : "true", tmp.s); args->flt_fail = bcf_hdr_id2int(args->hdr,BCF_DT_ID,flt_name.s); assert( args->flt_fail>=0 ); free(flt_name.s); free(tmp.s); } if ( args->snp_gap || args->indel_gap ) { if ( !args->filter_logic && args->soft_filter && strcmp(args->soft_filter,"+") ) { kstring_t tmp = {0,0,0}; if ( args->snp_gap ) kputs("\"SnpGap\"", &tmp); if ( args->indel_gap ) { if ( tmp.s ) kputs(" and ", &tmp); kputs("\"IndelGap\"", &tmp); } fprintf(stderr,"Warning: using %s filter name instead of \"%s\"\n", tmp.s,args->soft_filter); free(tmp.s); } rbuf_init(&args->rbuf, 64); args->rbuf_lines = (bcf1_t**) calloc(args->rbuf.m, sizeof(bcf1_t*)); if ( args->snp_gap ) { bcf_hdr_printf(args->hdr, "##FILTER=", args->snp_gap); args->SnpGap_id = bcf_hdr_id2int(args->hdr, BCF_DT_ID, "SnpGap"); assert( args->SnpGap_id>=0 ); } if ( args->indel_gap ) { bcf_hdr_printf(args->hdr, "##FILTER=", args->indel_gap); args->IndelGap_id = bcf_hdr_id2int(args->hdr, BCF_DT_ID, "IndelGap"); assert( args->IndelGap_id>=0 ); } } bcf_hdr_append_version(args->hdr, args->argc, args->argv, "bcftools_filter"); if ( args->filter_str ) args->filter = filter_init(args->hdr, args->filter_str); } static void destroy_data(args_t *args) { if ( args->rbuf_lines ) { int i; for (i=0; irbuf.m; i++) if ( args->rbuf_lines[i] ) bcf_destroy1(args->rbuf_lines[i]); free(args->rbuf_lines); } if ( args->filter ) filter_destroy(args->filter); free(args->tmpi); free(args->tmp_ac); } static void flush_buffer(args_t *args, int n) { int i, j; for (i=0; irbuf); bcf1_t *rec = args->rbuf_lines[k]; int pass = 1; if ( !args->soft_filter ) { for (j=0; jd.n_flt; j++) { if ( args->indel_gap && rec->d.flt[j]==args->IndelGap_id ) { pass = 0; break; } if ( args->snp_gap && rec->d.flt[j]==args->SnpGap_id ) { pass = 0; break; } } } if ( pass ) bcf_write1(args->out_fh, args->hdr, rec); } } #define SWAP(type_t, a, b) { type_t t = a; a = b; b = t; } static void buffered_filters(args_t *args, bcf1_t *line) { /** * The logic of SnpGap=3. The SNPs at positions 1 and 7 are filtered, * positions 0 and 8 are not: * 0123456789 * ref .G.GT..G.. * del .A.G-..A.. * Here the positions 1 and 6 are filtered, 0 and 7 are not: * 0123-456789 * ref .G.G-..G.. * ins .A.GT..A.. * * The logic of IndelGap=2. The second indel is filtered: * 012345678901 * ref .GT.GT..GT.. * del .G-.G-..G-.. * And similarly here, the second is filtered: * 01 23 456 78 * ref .A-.A-..A-.. * ins .AT.AT..AT.. */ // To avoid additional data structure, we abuse bcf1_t's var and var_type records. const int SnpGap_set = VCF_OTHER<<1; const int IndelGap_set = VCF_OTHER<<2; const int IndelGap_flush = VCF_OTHER<<3; int var_type = 0, i; if ( line ) { // Still on the same chromosome? int ilast = rbuf_last(&args->rbuf); if ( ilast>=0 && line->rid != args->rbuf_lines[ilast]->rid ) flush_buffer(args, args->rbuf.n); // new chromosome, flush everything if ( args->rbuf.n >= args->rbuf.m ) rbuf_expand0(&args->rbuf,bcf1_t*,args->rbuf_lines); // Insert the new record in the buffer. The line would be overwritten in // the next bcf_sr_next_line call, therefore we need to swap it with an // unused one ilast = rbuf_append(&args->rbuf); if ( !args->rbuf_lines[ilast] ) args->rbuf_lines[ilast] = bcf_init1(); SWAP(bcf1_t*, args->files->readers[0].buffer[0], args->rbuf_lines[ilast]); var_type = bcf_get_variant_types(line); // Find out the size of an indel. The indel boundaries are based on REF // (POS+1,POS+rlen-1). This is not entirely correct: mpileup likes to // output REF=CAGAGAGAGA, ALT=CAGAGAGAGAGA where REF=C,ALT=CGA could be // used. This filter is therefore more strict and may remove some valid // SNPs. int len = 1; if ( var_type & VCF_INDEL ) { for (i=1; in_allele; i++) if ( len < 1-line->d.var[i].n ) len = 1-line->d.var[i].n; } // Set the REF allele's length to max deletion length or to 1 if a SNP or an insertion. line->d.var[0].n = len; } int k_flush = 1; if ( args->indel_gap ) { k_flush = 0; // Find indels which are too close to each other int last_to = -1; for (i=-1; rbuf_next(&args->rbuf,&i); ) { bcf1_t *rec = args->rbuf_lines[i]; int rec_from = rec->pos; if ( last_to!=-1 && last_to < rec_from ) break; k_flush++; if ( !(rec->d.var_type & VCF_INDEL) ) continue; rec->d.var_type |= IndelGap_set; last_to = args->indel_gap + rec->pos + rec->d.var[0].n - 1; } if ( i==args->rbuf.f && line && last_to!=-1 ) k_flush = 0; if ( k_flush || !line ) { // Select the best indel from the cluster of k_flush indels int k = 0, max_ac = -1, imax_ac = -1; for (i=-1; rbuf_next(&args->rbuf,&i) && krbuf_lines[i]; if ( !(rec->d.var_type & IndelGap_set) ) continue; hts_expand(int, rec->n_allele, args->ntmpi, args->tmpi); int ret = bcf_calc_ac(args->hdr, rec, args->tmpi, BCF_UN_ALL); if ( imax_ac==-1 || (ret && max_ac < args->tmpi[1]) ) { max_ac = args->tmpi[1]; imax_ac = i; } } // Filter all but the best indel (with max AF or first if AF not available) k = 0; for (i=-1; rbuf_next(&args->rbuf,&i) && krbuf_lines[i]; if ( !(rec->d.var_type & IndelGap_set) ) continue; rec->d.var_type |= IndelGap_flush; if ( i!=imax_ac ) bcf_add_filter(args->hdr, args->rbuf_lines[i], args->IndelGap_id); } } } if ( !line ) { // Finished: flush everything flush_buffer(args, args->rbuf.n); return; } int j_flush = 1; if ( args->snp_gap ) { j_flush = 0; int last_from = line->pos; for (i=-1; rbuf_next(&args->rbuf,&i); ) { bcf1_t *rec = args->rbuf_lines[i]; int rec_to = rec->pos + rec->d.var[0].n - 1; // last position affected by the variant if ( rec_to + args->snp_gap < last_from ) j_flush++; else if ( (var_type & VCF_INDEL) && (rec->d.var_type & VCF_SNP) && !(rec->d.var_type & SnpGap_set) ) { // this SNP has not been SnpGap-filtered yet rec->d.var_type |= SnpGap_set; bcf_add_filter(args->hdr, rec, args->SnpGap_id); } else if ( (var_type & VCF_SNP) && (rec->d.var_type & VCF_INDEL) ) { // the line which we are adding is a SNP and needs to be filtered line->d.var_type |= SnpGap_set; bcf_add_filter(args->hdr, line, args->SnpGap_id); break; } } } flush_buffer(args, j_flush < k_flush ? j_flush : k_flush); } static void set_genotypes(args_t *args, bcf1_t *line, int pass_site) { int i,j; if ( !bcf_hdr_nsamples(args->hdr) ) return; if ( args->smpl_pass ) { int npass = 0; for (i=0; ihdr); i++) npass += args->smpl_pass[i]; // return if all samples pass if ( npass==bcf_hdr_nsamples(args->hdr) && (args->filter_logic & FLT_INCLUDE) ) return; if ( npass==0 && (args->filter_logic & FLT_EXCLUDE) ) return; } else if ( pass_site ) return; int an = 0, has_an = bcf_get_info_int32(args->hdr, line, "AN", &args->tmp_ac, &args->ntmp_ac); if ( has_an==1 ) an = args->tmp_ac[0]; else has_an = 0; int has_ac = bcf_get_info_int32(args->hdr, line, "AC", &args->tmp_ac, &args->ntmp_ac); has_ac = has_ac==line->n_allele-1 ? 1 : 0; int new_gt = 0, ngts = bcf_get_format_int32(args->hdr, line, "GT", &args->tmpi, &args->ntmpi); ngts /= bcf_hdr_nsamples(args->hdr); if ( args->set_gts==SET_GTS_MISSING ) new_gt = bcf_gt_missing; else if ( args->set_gts==SET_GTS_REF ) new_gt = bcf_gt_unphased(0); else error("todo: set_gts=%d\n", args->set_gts); for (i=0; ihdr); i++) { if ( args->smpl_pass ) { int pass = args->smpl_pass[i]; if ( args->filter_logic & FLT_EXCLUDE ) pass = pass ? 0 : 1; if ( pass ) continue; } int32_t *gts = args->tmpi + ngts*i; for (j=0; jset_gts==SET_GTS_MISSING && !bcf_gt_is_missing(gts[j]) ) { int ial = bcf_gt_allele(gts[j]); if ( has_ac && ial>0 && ial<=line->n_allele ) args->tmp_ac[ ial-1 ]--; an--; } else if ( args->set_gts==SET_GTS_REF ) { int ial = bcf_gt_allele(gts[j]); if ( bcf_gt_is_missing(gts[j]) ) an++; else if ( has_ac && ial>0 && ial<=line->n_allele ) args->tmp_ac[ ial-1 ]--; } gts[j] = new_gt; } } bcf_update_genotypes(args->hdr,line,args->tmpi,ngts*bcf_hdr_nsamples(args->hdr)); if ( has_an ) bcf_update_info_int32(args->hdr,line,"AN",&an,1); if ( has_ac ) bcf_update_info_int32(args->hdr,line,"AC",args->tmp_ac,line->n_allele-1); } static void usage(args_t *args) { fprintf(stderr, "\n"); fprintf(stderr, "About: Apply fixed-threshold filters.\n"); fprintf(stderr, "Usage: bcftools filter [options] \n"); fprintf(stderr, "\n"); fprintf(stderr, "Options:\n"); fprintf(stderr, " -e, --exclude exclude sites for which the expression is true (see man page for details)\n"); fprintf(stderr, " -g, --SnpGap filter SNPs within base pairs of an indel\n"); fprintf(stderr, " -G, --IndelGap filter clusters of indels separated by or fewer base pairs allowing only one to pass\n"); fprintf(stderr, " -i, --include include only sites for which the expression is true (see man page for details\n"); fprintf(stderr, " -m, --mode [+x] \"+\": do not replace but add to existing FILTER; \"x\": reset filters at sites which pass\n"); fprintf(stderr, " -o, --output write output to a file [standard output]\n"); fprintf(stderr, " -O, --output-type b: compressed BCF, u: uncompressed BCF, z: compressed VCF, v: uncompressed VCF [v]\n"); fprintf(stderr, " -r, --regions restrict to comma-separated list of regions\n"); fprintf(stderr, " -R, --regions-file restrict to regions listed in a file\n"); fprintf(stderr, " -s, --soft-filter annotate FILTER column with or unique filter name (\"Filter%%d\") made up by the program (\"+\")\n"); fprintf(stderr, " -S, --set-GTs <.|0> set genotypes of failed samples to missing (.) or ref (0)\n"); fprintf(stderr, " -t, --targets similar to -r but streams rather than index-jumps\n"); fprintf(stderr, " -T, --targets-file similar to -R but streams rather than index-jumps\n"); fprintf(stderr, "\n"); exit(1); } int main_vcffilter(int argc, char *argv[]) { int c; args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->argc = argc; args->argv = argv; args->files = bcf_sr_init(); args->output_fname = "-"; args->output_type = FT_VCF; int regions_is_file = 0, targets_is_file = 0; static struct option loptions[] = { {"set-GTs",1,0,'S'}, {"mode",1,0,'m'}, {"soft-filter",1,0,'s'}, {"exclude",1,0,'e'}, {"include",1,0,'i'}, {"targets",1,0,'t'}, {"targets-file",1,0,'T'}, {"regions",1,0,'r'}, {"regions-file",1,0,'R'}, {"output",1,0,'o'}, {"output-type",1,0,'O'}, {"SnpGap",1,0,'g'}, {"IndelGap",1,0,'G'}, {0,0,0,0} }; char *tmp; while ((c = getopt_long(argc, argv, "e:i:t:T:r:R:h?s:m:o:O:g:G:S:",loptions,NULL)) >= 0) { switch (c) { case 'g': args->snp_gap = strtol(optarg,&tmp,10); if ( *tmp ) error("Could not parse argument: --SnpGap %s\n", optarg); break; case 'G': args->indel_gap = strtol(optarg,&tmp,10); if ( *tmp ) error("Could not parse argument: --IndelGap %s\n", optarg); break; case 'o': args->output_fname = optarg; break; case 'O': switch (optarg[0]) { case 'b': args->output_type = FT_BCF_GZ; break; case 'u': args->output_type = FT_BCF; break; case 'z': args->output_type = FT_VCF_GZ; break; case 'v': args->output_type = FT_VCF; break; default: error("The output type \"%s\" not recognised\n", optarg); } break; case 's': args->soft_filter = optarg; break; case 'm': if ( strchr(optarg,'x') ) args->annot_mode |= ANNOT_RESET; if ( strchr(optarg,'+') ) args->annot_mode |= ANNOT_ADD; break; case 't': args->targets_list = optarg; break; case 'T': args->targets_list = optarg; targets_is_file = 1; break; case 'r': args->regions_list = optarg; break; case 'R': args->regions_list = optarg; regions_is_file = 1; break; case 'e': args->filter_str = optarg; args->filter_logic |= FLT_EXCLUDE; break; case 'i': args->filter_str = optarg; args->filter_logic |= FLT_INCLUDE; break; case 'S': if ( !strcmp(".",optarg) ) args->set_gts = SET_GTS_MISSING; else if ( !strcmp("0",optarg) ) args->set_gts = SET_GTS_REF; else error("The argument to -S not recognised: %s\n", optarg); break; case 'h': case '?': usage(args); default: error("Unknown argument: %s\n", optarg); } } if ( args->filter_logic == (FLT_EXCLUDE|FLT_INCLUDE) ) error("Only one of -i or -e can be given.\n"); char *fname = NULL; if ( optind>=argc ) { if ( !isatty(fileno((FILE *)stdin)) ) fname = "-"; // reading from stdin else usage(args); } else fname = argv[optind]; // read in the regions from the command line if ( args->regions_list ) { args->files->require_index = 1; if ( bcf_sr_set_regions(args->files, args->regions_list, regions_is_file)<0 ) error("Failed to read the regions: %s\n", args->regions_list); } else if ( optind+1 < argc ) { int i; kstring_t tmp = {0,0,0}; kputs(argv[optind+1],&tmp); for (i=optind+2; ifiles->require_index = 1; if ( bcf_sr_set_regions(args->files, tmp.s, regions_is_file)<0 ) error("Failed to read the regions: %s\n", args->regions_list); free(tmp.s); } if ( args->targets_list ) { if ( bcf_sr_set_targets(args->files, args->targets_list,targets_is_file, 0)<0 ) error("Failed to read the targets: %s\n", args->targets_list); } if ( !bcf_sr_add_reader(args->files, fname) ) error("Failed to open %s: %s\n", fname,bcf_sr_strerror(args->files->errnum)); init_data(args); bcf_hdr_write(args->out_fh, args->hdr); while ( bcf_sr_next_line(args->files) ) { bcf1_t *line = bcf_sr_get_line(args->files, 0); int pass = 1; if ( args->filter ) { pass = filter_test(args->filter, line, &args->smpl_pass); if ( args->filter_logic & FLT_EXCLUDE ) pass = pass ? 0 : 1; } if ( args->soft_filter || args->set_gts || pass ) { if ( pass ) { bcf_unpack(line,BCF_UN_FLT); if ( args->annot_mode & ANNOT_RESET || !line->d.n_flt ) bcf_add_filter(args->hdr, line, args->flt_pass); } else if ( args->soft_filter ) { if ( (args->annot_mode & ANNOT_ADD) ) bcf_add_filter(args->hdr, line, args->flt_fail); else bcf_update_filter(args->hdr, line, &args->flt_fail, 1); } if ( args->set_gts ) set_genotypes(args, line, pass); if ( !args->rbuf_lines ) bcf_write1(args->out_fh, args->hdr, line); else buffered_filters(args, line); } } buffered_filters(args, NULL); hts_close(args->out_fh); destroy_data(args); bcf_sr_destroy(args->files); free(args); return 0; } bcftools-1.2/vcfgtcheck.c000066400000000000000000001011161246371514100154460ustar00rootroot00000000000000/* vcfgtcheck.c -- Check sample identity. Copyright (C) 2013-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include #include #include #include #include "bcftools.h" typedef struct { bcf_srs_t *files; // first reader is the query VCF - single sample normally or multi-sample for cross-check bcf_hdr_t *gt_hdr, *sm_hdr; // VCF with genotypes to compare against and the query VCF int ntmp_arr, npl_arr; int32_t *tmp_arr, *pl_arr; double *lks, *sites; int *cnts, *dps, hom_only, cross_check, all_sites; char *cwd, **argv, *gt_fname, *plot, *query_sample, *target_sample; int argc, no_PLs; } args_t; FILE *open_file(char **fname, const char *mode, const char *fmt, ...); char *msprintf(const char *fmt, ...); void mkdir_p(const char *fmt, ...); void py_plot(char *script) { mkdir_p(script); int len = strlen(script); char *cmd = !strcmp(".py",script+len-3) ? msprintf("python %s", script) : msprintf("python %s.py", script); int ret = system(cmd); if ( ret ) fprintf(stderr, "The command returned non-zero status %d: %s\n", ret, cmd); free(cmd); } static void plot_check(args_t *args, char *target_sample, char *query_sample) { char *fname; FILE *fp = open_file(&fname, "w", "%s.py", args->plot); fprintf(fp, "import matplotlib as mpl\n" "mpl.use('Agg')\n" "import matplotlib.pyplot as plt\n" "import matplotlib.gridspec as gridspec\n" "import csv\n" "csv.register_dialect('tab', delimiter='\\t', quoting=csv.QUOTE_NONE)\n" "\n" "sample_ids = False\n" "\n" "dat = []\n" "with open('%s.tab', 'rb') as f:\n" " reader = csv.reader(f, 'tab')\n" " for row in reader:\n" " if row[0][0]=='#': continue\n" " if row[0]!='CN': continue\n" " tgt = 0\n" " if row[4]=='%s': tgt = 1\n" " dat.append([float(row[1]), float(row[2]), float(row[3]), tgt, row[4]])\n" "\n" "dat = sorted(dat, reverse=True)\n" "\n" "iq = -1; dp = 0\n" "for i in range(len(dat)):\n" " if iq==-1 and dat[i][3]==1: iq = i\n" " dp += dat[i][2]\n" "dp /= len(dat)\n" "\n" "fig,ax1 = plt.subplots(figsize=(8,5))\n" "ax2 = ax1.twinx()\n" "plots = ax1.plot([x[0] for x in dat],'o-', ms=3, color='g', mec='g', label='Discordance (total)')\n" "plots += ax1.plot([x[1] for x in dat], '^', ms=3, color='r', mec='r', label='Discordance (per site)')\n" "plots += ax2.plot([x[2] for x in dat],'v', ms=3, color='k', label='Number of sites')\n" "if iq!=-1:\n" " ax1.plot([iq],[dat[iq][0]],'o',color='orange', ms=9)\n" " ax1.annotate('%s',xy=(iq,dat[iq][0]), xytext=(5,5), textcoords='offset points',fontsize='xx-small',rotation=45,va='bottom',ha='left')\n" " ax1.plot([iq],[dat[iq][1]],'^',color='red', ms=5)\n" "for tl in ax1.get_yticklabels(): tl.set_color('g')\n" "for tl in ax2.get_yticklabels(): tl.set_color('k'); tl.set_fontsize(9)\n" "ax1.set_title('Discordance with %s')\n" "ax1.set_xlim(-0.05*len(dat),1.05*(len(dat)-1))\n" "ax1.set_xlabel('Sample ID')\n" "plt.subplots_adjust(left=0.1,right=0.9,bottom=0.1,top=0.9)\n" "if sample_ids:\n" " ax1.set_xticks(range(len(dat)))\n" " ax1.set_xticklabels([x[4] for x in dat],**{'rotation':45, 'ha':'right', 'fontsize':8})\n" " plt.subplots_adjust(bottom=0.2)\n" "ax1.set_ylabel('Discordance',color='g')\n" "ax2.set_ylabel('Number of sites',color='k')\n" "ax2.ticklabel_format(style='sci', scilimits=(-3,2), axis='y')\n" "ax1.ticklabel_format(style='sci', scilimits=(-3,2), axis='y')\n" "labels = [l.get_label() for l in plots]\n" "plt.legend(plots,labels,numpoints=1,markerscale=1,loc='best',prop={'size':10},frameon=False)\n" "plt.savefig('%s.png')\n" "plt.close()\n" "\n", args->plot, target_sample, target_sample, query_sample, args->plot ); fclose(fp); py_plot(fname); free(fname); } static void plot_cross_check(args_t *args) { char *fname; FILE *fp = open_file(&fname, "w", "%s.py", args->plot); fprintf(fp, "import matplotlib as mpl\n" "mpl.use('Agg')\n" "import matplotlib.pyplot as plt\n" "import matplotlib.gridspec as gridspec\n" "import csv\n" "csv.register_dialect('tab', delimiter='\\t', quoting=csv.QUOTE_NONE)\n" "avg = []\n" "dp = []\n" "sm2id = {}\n" "dat = None\n" "min = None\n" "max = None\n" "with open('%s.tab', 'rb') as f:\n" " reader = csv.reader(f, 'tab')\n" " i = 0\n" " for row in reader:\n" " if row[0]=='SM':\n" " sm2id[row[4]] = i\n" " avg.append([i,float(row[1])])\n" " dp.append([i,float(row[2])])\n" " i += 1\n" " elif row[0]=='CN':\n" " val = 0\n" " if int(row[2])!=0: val = float(row[1])/int(row[2])\n" " if not dat:\n" " dat = [[0]*len(sm2id) for x in xrange(len(sm2id))]\n" " min = val\n" " max = val\n" " id_i = sm2id[row[4]]\n" " id_j = sm2id[row[5]]\n" " dat[id_i][id_j] = val\n" " dat[id_j][id_i] = val\n" " if min > val: min = val\n" " if max < val: max = val\n" "\n" "if len(sm2id)<=1: exit(1)\n" "if min==max: exit(1)\n" "\n" "fig = plt.figure(figsize=(6,7))\n" "gs = gridspec.GridSpec(2, 1, height_ratios=[1, 1.5])\n" "ax1 = plt.subplot(gs[0])\n" "ax2 = plt.subplot(gs[1])\n" "\n" "ax1.plot([x[0] for x in avg],[x[1] for x in avg],'^-', ms=3, color='k')\n" "ax3 = ax1.twinx()\n" "ax3.plot([x[0] for x in dp],[x[1] for x in dp],'^-', ms=3, color='r',mec='r')\n" "for tl in ax3.get_yticklabels():\n" " tl.set_color('r')\n" " tl.set_fontsize(9)\n" "\n" "im = ax2.imshow(dat,clim=(min),interpolation='nearest',origin='lower')\n" "cb1 = plt.colorbar(im,ax=ax2)\n" "cb1.set_label('Pairwise discordance')\n" "for t in cb1.ax.get_yticklabels(): t.set_fontsize(9)\n" "\n" "ax1.tick_params(axis='both', which='major', labelsize=9)\n" "ax1.tick_params(axis='both', which='minor', labelsize=9)\n" "ax2.tick_params(axis='both', which='major', labelsize=9)\n" "ax2.tick_params(axis='both', which='minor', labelsize=9)\n" "\n" "ax1.set_title('Sample Discordance Score')\n" "ax2.set_ylabel('Sample ID')\n" "ax2.set_xlabel('Sample ID')\n" "ax3.set_ylabel('Average Depth',color='r')\n" "ax1.set_xlabel('Sample ID')\n" "ax1.set_ylabel('Average discordance')\n" "\n" "plt.subplots_adjust(left=0.15,right=0.87,bottom=0.08,top=0.93,hspace=0.25)\n" "plt.savefig('%s.png')\n" "plt.close()\n" "\n", args->plot,args->plot ); fclose(fp); py_plot(fname); free(fname); } static void init_data(args_t *args) { args->sm_hdr = args->files->readers[0].header; if ( !bcf_hdr_nsamples(args->sm_hdr) ) error("No samples in %s?\n", args->files->readers[0].fname); if ( !args->cross_check ) { args->gt_hdr = args->files->readers[1].header; int nsamples = bcf_hdr_nsamples(args->gt_hdr); if ( !nsamples ) error("No samples in %s?\n", args->files->readers[1].fname); args->lks = (double*) calloc(nsamples,sizeof(double)); args->cnts = (int*) calloc(nsamples,sizeof(int)); args->sites = (double*) calloc(nsamples,sizeof(double)); args->dps = (int*) calloc(nsamples,sizeof(int)); } else { int nsamples = bcf_hdr_nsamples(args->sm_hdr); int narr = (nsamples-1)*nsamples/2; args->lks = (double*) calloc(narr,sizeof(double)); args->cnts = (int*) calloc(narr,sizeof(int)); args->dps = (int*) calloc(narr,sizeof(int)); } } static void destroy_data(args_t *args) { free(args->lks); free(args->cnts); free(args->dps); free(args->cwd); free(args->sites); } static int allele_to_int(bcf1_t *line, char *allele) { int i; for (i=0; in_allele; i++) if ( !strcmp(allele,line->d.allele[i]) ) return i; if ( strcmp(line->d.allele[i-1],"X") ) return -1; return i-1; } static int init_gt2ipl(args_t *args, bcf1_t *gt_line, bcf1_t *sm_line, int *gt2ipl, int n_gt2ipl) { int i, j; for (i=0; in_allele; i++) { // find which of the sm_alleles (k) corresponds to the gt_allele (i) int k = allele_to_int(sm_line, gt_line->d.allele[i]); if ( k<0 ) return 0; for (j=0; j<=i; j++) { int l = allele_to_int(sm_line, gt_line->d.allele[j]); if ( l<0 ) return 0; gt2ipl[ bcf_ij2G(j,i) ] = k<=l ? bcf_ij2G(k,l) : bcf_ij2G(l,k); } } //for (i=0; icwd = (char*) malloc(sizeof(char)*nbuf); for (i=0; i<5; i++) { if ( (buf = getcwd(args->cwd, nbuf)) ) break; nbuf *= 2; args->cwd = (char*) realloc(args->cwd, sizeof(char)*nbuf); } assert(buf); } static void print_header(args_t *args, FILE *fp) { fprintf(fp, "# This file was produced by bcftools (%s+htslib-%s), the command line was:\n", bcftools_version(), hts_version()); fprintf(fp, "# \t bcftools %s ", args->argv[0]); int i; for (i=1; iargc; i++) fprintf(fp, " %s",args->argv[i]); fprintf(fp, "\n# and the working directory was:\n"); fprintf(fp, "# \t %s\n#\n", args->cwd); } static int fake_PLs(args_t *args, bcf_hdr_t *hdr, bcf1_t *line) { // PLs not present, use GTs instead. int fake_PL = args->no_PLs ? args->no_PLs : 99; // with 1, discordance is the number of non-matching GTs int nsm_gt, i; if ( (nsm_gt=bcf_get_genotypes(hdr, line, &args->tmp_arr, &args->ntmp_arr)) <= 0 ) error("GT not present at %s:%d?\n", hdr->id[BCF_DT_CTG][line->rid].key, line->pos+1); nsm_gt /= bcf_hdr_nsamples(hdr); int npl = line->n_allele*(line->n_allele+1)/2; hts_expand(int,npl*bcf_hdr_nsamples(hdr),args->npl_arr,args->pl_arr); for (i=0; itmp_arr + i*nsm_gt; int j, *pl_ptr = args->pl_arr + i*npl; if ( bcf_gt_is_missing(gt_ptr[0]) || bcf_gt_is_missing(gt_ptr[1]) ) // missing genotype { for (j=0; jno_PLs; // Initialize things: check which tags are defined in the header, sample names etc. if ( bcf_hdr_id2int(args->gt_hdr, BCF_DT_ID, "GT")<0 ) error("[E::%s] GT not present in the header of %s?\n", __func__, args->files->readers[1].fname); if ( bcf_hdr_id2int(args->sm_hdr, BCF_DT_ID, "PL")<0 ) { if ( bcf_hdr_id2int(args->sm_hdr, BCF_DT_ID, "GT")<0 ) error("[E::%s] Neither PL nor GT present in the header of %s\n", __func__, args->files->readers[0].fname); if ( !args->no_PLs ) fprintf(stderr,"Warning: PL not present in the header of %s, using GT instead\n", args->files->readers[0].fname); fake_pls = 1; } FILE *fp = args->plot ? open_file(NULL, "w", "%s.tab", args->plot) : stdout; print_header(args, fp); int tgt_isample = -1, query_isample = 0; if ( args->target_sample ) { tgt_isample = bcf_hdr_id2int(args->gt_hdr, BCF_DT_SAMPLE, args->target_sample); if ( tgt_isample<0 ) error("No such sample in %s: [%s]\n", args->files->readers[1].fname, args->target_sample); } if ( args->all_sites ) { if ( tgt_isample==-1 ) { fprintf(stderr,"No target sample selected for comparison, using the first sample in %s: %s\n", args->gt_fname,args->gt_hdr->samples[0]); tgt_isample = 0; } } if ( args->query_sample ) { query_isample = bcf_hdr_id2int(args->sm_hdr, BCF_DT_SAMPLE, args->query_sample); if ( query_isample<0 ) error("No such sample in %s: [%s]\n", args->files->readers[0].fname, args->query_sample); } if ( args->all_sites ) fprintf(fp, "# [1]SC, Site by Site Comparison\t[2]Chromosome\t[3]Position\t[4]-g alleles\t[5]-g GT (%s)\t[6]match log LK\t[7]Query alleles\t[8-]Query PLs (%s)\n", args->gt_hdr->samples[tgt_isample],args->sm_hdr->samples[query_isample]); // Main loop float prev_lk = 0; while ( (ret=bcf_sr_next_line(args->files)) ) { if ( ret!=2 ) continue; bcf1_t *sm_line = args->files->readers[0].buffer[0]; // the query file bcf1_t *gt_line = args->files->readers[1].buffer[0]; // the -g target file bcf_unpack(sm_line, BCF_UN_FMT); bcf_unpack(gt_line, BCF_UN_FMT); // Init mapping from target genotype index to the sample's PL fields int n_gt2ipl = gt_line->n_allele*(gt_line->n_allele + 1)/2; if ( n_gt2ipl > m_gt2ipl ) { m_gt2ipl = n_gt2ipl; gt2ipl = (int*) realloc(gt2ipl, sizeof(int)*m_gt2ipl); } if ( !init_gt2ipl(args, gt_line, sm_line, gt2ipl, n_gt2ipl) ) continue; // Target genotypes int ngt, npl; if ( (ngt=bcf_get_genotypes(args->gt_hdr, gt_line, >_arr, &ngt_arr)) <= 0 ) error("GT not present at %s:%d?", args->gt_hdr->id[BCF_DT_CTG][gt_line->rid].key, gt_line->pos+1); ngt /= bcf_hdr_nsamples(args->gt_hdr); if ( ngt!=2 ) continue; // checking only diploid genotypes // Sample PLs if ( !fake_pls ) { if ( (npl=bcf_get_format_int32(args->sm_hdr, sm_line, "PL", &args->pl_arr, &args->npl_arr)) <= 0 ) error("PL not present at %s:%d?", args->sm_hdr->id[BCF_DT_CTG][sm_line->rid].key, sm_line->pos+1); npl /= bcf_hdr_nsamples(args->sm_hdr); } else npl = fake_PLs(args, args->sm_hdr, sm_line); // Calculate likelihoods for all samples, assuming diploid genotypes // For faster access to genotype likelihoods (PLs) of the query sample int max_ipl, *pl_ptr = args->pl_arr + query_isample*npl; double sum_pl = 0; // for converting PLs to probs for (max_ipl=0; max_iplno_PLs==1 ) sum_pl = -1; // The main stats: concordance of the query sample with the target -g samples for (i=0; igt_hdr); i++) { int *gt_ptr = gt_arr + i*ngt; if ( gt_ptr[1]==bcf_int32_vector_end ) continue; // skip haploid genotypes if ( bcf_gt_is_missing(gt_ptr[0]) || bcf_gt_is_missing(gt_ptr[1]) ) continue; int a = bcf_gt_allele(gt_ptr[0]); int b = bcf_gt_allele(gt_ptr[1]); if ( args->hom_only && a!=b ) continue; // heterozygous genotype int igt_tgt = igt_tgt = bcf_alleles2gt(a,b); // genotype index in the target file int igt_qry = gt2ipl[igt_tgt]; // corresponding genotype in query file if ( igt_qry>=max_ipl || pl_ptr[igt_qry]<0 ) continue; // genotype not present in query sample: haploid or missing args->lks[i] += sum_pl<0 ? -pl_ptr[igt_qry] : log(pow(10, -0.1*pl_ptr[igt_qry])/sum_pl); args->sites[i]++; } if ( args->all_sites ) { // Print LKs at all sites for debugging int *gt_ptr = gt_arr + tgt_isample*ngt; if ( gt_ptr[1]==bcf_int32_vector_end ) continue; // skip haploid genotypes int a = bcf_gt_allele(gt_ptr[0]); int b = bcf_gt_allele(gt_ptr[1]); if ( args->hom_only && a!=b ) continue; // heterozygous genotype fprintf(fp, "SC\t%s\t%d", args->gt_hdr->id[BCF_DT_CTG][gt_line->rid].key, gt_line->pos+1); for (i=0; in_allele; i++) fprintf(fp, "%c%s", i==0?'\t':',', gt_line->d.allele[i]); fprintf(fp, "\t%s/%s", a>=0 ? gt_line->d.allele[a] : ".", b>=0 ? gt_line->d.allele[b] : "."); fprintf(fp, "\t%f", args->lks[query_isample]-prev_lk); prev_lk = args->lks[query_isample]; int igt, *pl_ptr = args->pl_arr + query_isample*npl; // PLs of the query sample for (i=0; in_allele; i++) fprintf(fp, "%c%s", i==0?'\t':',', sm_line->d.allele[i]); for (igt=0; igtpl_arr); free(args->tmp_arr); // To be able to plot total discordance (=number of mismatching GTs with -G1) in the same // plot as discordance per site, the latter must be scaled to the same range int nsamples = bcf_hdr_nsamples(args->gt_hdr); double extreme_lk = 0, extreme_lk_per_site = 0; for (i=0; ilks[i] < extreme_lk ) extreme_lk = args->lks[i]; if ( args->sites[i] && args->lks[i]/args->sites[i] < extreme_lk_per_site ) extreme_lk_per_site = args->lks[i]/args->sites[i]; } // Sorted output double **p = (double**) malloc(sizeof(double*)*nsamples); for (i=0; ilks[i]; qsort(p, nsamples, sizeof(int*), cmp_doubleptr); fprintf(fp, "# [1]CN\t[2]Discordance with %s (total)\t[3]Discordance (score per site)\t[4]Number of sites compared\t[5]Sample\t[6]Sample ID\n", args->sm_hdr->samples[query_isample]); for (i=0; ilks; double per_site = 0; if ( args->sites[idx] ) { if ( args->sites[idx] && extreme_lk_per_site ) { per_site = args->lks[idx]/args->sites[idx]; per_site *= extreme_lk / extreme_lk_per_site; } else per_site = 0; } fprintf(fp, "CN\t%e\t%e\t%.0f\t%s\t%d\n", fabs(args->lks[idx]), fabs(per_site), args->sites[idx], args->gt_hdr->samples[idx], i); } if ( args->plot ) { fclose(fp); plot_check(args, args->target_sample ? args->target_sample : "", args->sm_hdr->samples[query_isample]); } } static inline int is_hom_most_likely(int nals, int *pls) { int ia, ib, idx = 1, min_is_hom = 1, min_pl = pls[0]; for (ia=1; iasm_hdr), ndp_arr = 0; unsigned int *dp = (unsigned int*) calloc(nsamples,sizeof(unsigned int)), *ndp = (unsigned int*) calloc(nsamples,sizeof(unsigned int)); // this will overflow one day... int fake_pls = args->no_PLs, ignore_dp = 0; int i,j,k,idx, pl_warned = 0, dp_warned = 0; int32_t *dp_arr = NULL; int *is_hom = args->hom_only ? (int*) malloc(sizeof(int)*nsamples) : NULL; if ( bcf_hdr_id2int(args->sm_hdr, BCF_DT_ID, "PL")<0 ) { if ( bcf_hdr_id2int(args->sm_hdr, BCF_DT_ID, "GT")<0 ) error("[E::%s] Neither PL nor GT present in the header of %s\n", __func__, args->files->readers[0].fname); if ( !args->no_PLs ) fprintf(stderr,"Warning: PL not present in the header of %s, using GT instead\n", args->files->readers[0].fname); fake_pls = 1; } if ( bcf_hdr_id2int(args->sm_hdr, BCF_DT_ID, "DP")<0 ) ignore_dp = 1; FILE *fp = args->plot ? open_file(NULL, "w", "%s.tab", args->plot) : stdout; print_header(args, fp); if ( args->all_sites ) fprintf(fp,"# [1]SD, Average Site Discordance\t[2]Chromosome\t[3]Position\t[4]Number of available pairs\t[5]Average discordance\n"); while ( bcf_sr_next_line(args->files) ) { bcf1_t *line = args->files->readers[0].buffer[0]; bcf_unpack(line, BCF_UN_FMT); int npl; if ( !fake_pls ) { npl = bcf_get_format_int32(args->sm_hdr, line, "PL", &args->pl_arr, &args->npl_arr); if ( npl<=0 ) { pl_warned++; continue; } npl /= nsamples; } else npl = fake_PLs(args, args->sm_hdr, line); if ( !ignore_dp && bcf_get_format_int32(args->sm_hdr, line, "DP", &dp_arr, &ndp_arr) <= 0 ) { dp_warned++; continue; } if ( args->hom_only ) { for (i=0; in_allele, args->pl_arr+i*npl); } double sum = 0; int nsum = 0; idx = 0; for (i=0; ipl_arr[i*npl]; if ( *ipl==-1 ) { idx += i; continue; } // missing genotype if ( !ignore_dp && (dp_arr[i]==bcf_int32_missing || !dp_arr[i]) ) { idx += i; continue; } if ( args->hom_only && !is_hom[i] ) { idx += i; continue; } for (j=0; jpl_arr[j*npl]; if ( *jpl==-1 ) { idx++; continue; } // missing genotype if ( !ignore_dp && (dp_arr[j]==bcf_int32_missing || !dp_arr[j]) ) { idx++; continue; } if ( args->hom_only && !is_hom[j] ) { idx++; continue; } int min_pl = INT_MAX; for (k=0; k ipl[k]+jpl[k] ) min_pl = ipl[k]+jpl[k]; } if ( k!=npl ) { idx++; continue; } if ( args->all_sites ) { sum += min_pl; nsum++; } args->lks[idx] += min_pl; args->cnts[idx]++; if ( !ignore_dp ) { args->dps[idx] += dp_arr[i] < dp_arr[j] ? dp_arr[i] : dp_arr[j]; dp[i] += dp_arr[i]; ndp[i]++; dp[j] += dp_arr[j]; ndp[j]++; } else { args->dps[idx]++; dp[i]++; ndp[i]++; dp[j]++; ndp[j]++; } idx++; } } if ( args->all_sites ) fprintf(fp,"SD\t%s\t%d\t%d\t%.0f\n", args->sm_hdr->id[BCF_DT_CTG][line->rid].key, line->pos+1, nsum, nsum?sum/nsum:0); } if ( dp_arr ) free(dp_arr); if ( args->pl_arr ) free(args->pl_arr); if ( args->tmp_arr ) free(args->tmp_arr); if ( is_hom ) free(is_hom); if ( pl_warned ) fprintf(stderr, "[W::%s] PL was not found at %d site(s)\n", __func__, pl_warned); if ( dp_warned ) fprintf(stderr, "[W::%s] DP was not found at %d site(s)\n", __func__, dp_warned); // Output samples sorted by average discordance double *score = (double*) calloc(nsamples,sizeof(double)); args->sites = (double*) calloc(nsamples,sizeof(double)); idx = 0; for (i=0; ilks[idx]; score[j] += args->lks[idx]; args->sites[i] += args->cnts[idx]; args->sites[j] += args->cnts[idx]; idx++; } } for (i=0; isites[i] ) score[i] /= args->sites[i]; double **p = (double**) malloc(sizeof(double*)*nsamples), avg_score = 0; for (i=0; isites[idx]/(nsamples-1); avg_score += score[idx]; fprintf(fp, "SM\t%f\t%.2lf\t%.0lf\t%s\t%d\n", score[idx]*100., adp, nsites, args->sm_hdr->samples[idx],i); } // // Overall score: maximum absolute deviation from the average score // fprintf(fp, "# [1] MD\t[2]Maximum deviation\t[3]The culprit\n"); // fprintf(fp, "MD\t%f\t%s\n", (score[idx] - avg_score/nsamples)*100., args->sm_hdr->samples[idx]); // idx still set free(p); free(score); free(dp); free(ndp); // Pairwise discordances fprintf(fp, "# [1]CN\t[2]Discordance\t[3]Number of sites\t[4]Average minimum depth\t[5]Sample i\t[6]Sample j\n"); idx = 0; for (i=0; ilks[idx], args->cnts[idx], args->cnts[idx]?(double)args->dps[idx]/args->cnts[idx]:0.0, args->sm_hdr->samples[i],args->sm_hdr->samples[j]); idx++; } } fclose(fp); if ( args->plot ) plot_cross_check(args); } static char *init_prefix(char *prefix) { int len = strlen(prefix); if ( prefix[len-1] == '/' || prefix[len-1] == '\\' ) return msprintf("%sgtcheck", prefix); return strdup(prefix); } static void usage(void) { fprintf(stderr, "\n"); fprintf(stderr, "About: Check sample identity. With no -g BCF given, multi-sample cross-check is performed.\n"); fprintf(stderr, "Usage: bcftools gtcheck [options] [-g ] \n"); fprintf(stderr, "\n"); fprintf(stderr, "Options:\n"); fprintf(stderr, " -a, --all-sites output comparison for all sites\n"); fprintf(stderr, " -g, --genotypes genotypes to compare against\n"); fprintf(stderr, " -G, --GTs-only use GTs, ignore PLs, using for unseen genotypes [99]\n"); fprintf(stderr, " -H, --homs-only homozygous genotypes only (useful for low coverage data)\n"); fprintf(stderr, " -p, --plot plot\n"); fprintf(stderr, " -r, --regions restrict to comma-separated list of regions\n"); fprintf(stderr, " -R, --regions-file restrict to regions listed in a file\n"); fprintf(stderr, " -s, --query-sample query sample (by default the first sample is checked)\n"); fprintf(stderr, " -S, --target-sample target sample in the -g file (used only for plotting)\n"); fprintf(stderr, " -t, --targets similar to -r but streams rather than index-jumps\n"); fprintf(stderr, " -T, --targets-file similar to -R but streams rather than index-jumps\n"); fprintf(stderr, "\n"); exit(1); } int main_vcfgtcheck(int argc, char *argv[]) { int c; args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->files = bcf_sr_init(); args->argc = argc; args->argv = argv; set_cwd(args); char *regions = NULL, *targets = NULL; int regions_is_file = 0, targets_is_file = 0; static struct option loptions[] = { {"GTs-only",1,0,'G'}, {"all-sites",0,0,'a'}, {"homs-only",0,0,'H'}, {"help",0,0,'h'}, {"genotypes",1,0,'g'}, {"plot",1,0,'p'}, {"target-sample",1,0,'S'}, {"query-sample",1,0,'s'}, {"regions",1,0,'r'}, {"regions-file",1,0,'R'}, {"targets",1,0,'t'}, {"targets-file",1,0,'T'}, {0,0,0,0} }; char *tmp; while ((c = getopt_long(argc, argv, "hg:p:s:S:Hr:R:at:T:G:",loptions,NULL)) >= 0) { switch (c) { case 'G': args->no_PLs = strtol(optarg,&tmp,10); if ( *tmp ) error("Could not parse argument: --GTs-only %s\n", optarg); break; case 'a': args->all_sites = 1; break; case 'H': args->hom_only = 1; break; case 'g': args->gt_fname = optarg; break; case 'p': args->plot = optarg; break; case 'S': args->target_sample = optarg; break; case 's': args->query_sample = optarg; break; case 'r': regions = optarg; break; case 'R': regions = optarg; regions_is_file = 1; break; case 't': targets = optarg; break; case 'T': targets = optarg; targets_is_file = 1; break; case 'h': case '?': usage(); default: error("Unknown argument: %s\n", optarg); } } char *fname = NULL; if ( optind==argc ) { if ( !isatty(fileno((FILE *)stdin)) ) fname = "-"; // reading from stdin else usage(); // no files given } else fname = argv[optind]; if ( argc>optind+1 ) usage(); // too many files given if ( !args->gt_fname ) args->cross_check = 1; // no genotype file, run in cross-check mode else args->files->require_index = 1; if ( regions && bcf_sr_set_regions(args->files, regions, regions_is_file)<0 ) error("Failed to read the regions: %s\n", regions); if ( targets && bcf_sr_set_targets(args->files, targets, targets_is_file, 0)<0 ) error("Failed to read the targets: %s\n", targets); if ( !bcf_sr_add_reader(args->files, fname) ) error("Failed to open %s: %s\n", fname,bcf_sr_strerror(args->files->errnum)); if ( args->gt_fname && !bcf_sr_add_reader(args->files, args->gt_fname) ) error("Failed to open %s: %s\n", args->gt_fname,bcf_sr_strerror(args->files->errnum)); args->files->collapse = COLLAPSE_SNPS|COLLAPSE_INDELS; if ( args->plot ) args->plot = init_prefix(args->plot); init_data(args); if ( args->cross_check ) cross_check_gts(args); else check_gt(args); destroy_data(args); bcf_sr_destroy(args->files); if (args->plot) free(args->plot); free(args); return 0; } bcftools-1.2/vcfindex.c000066400000000000000000000204361246371514100151520ustar00rootroot00000000000000 /* vcfindex.c -- Index bgzip compressed VCF/BCF files for random access. Copyright (C) 2014 Genome Research Ltd. Author: Shane McCarthy Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #define __STDC_FORMAT_MACROS #include #include "bcftools.h" #define BCF_LIDX_SHIFT 14 static void usage(void) { fprintf(stderr, "\n"); fprintf(stderr, "About: Index bgzip compressed VCF/BCF files for random access.\n"); fprintf(stderr, "Usage: bcftools index [options] |\n"); fprintf(stderr, "\n"); fprintf(stderr, "Indexing options:\n"); fprintf(stderr, " -c, --csi generate CSI-format index for VCF/BCF files [default]\n"); fprintf(stderr, " -f, --force overwrite index if it already exists\n"); fprintf(stderr, " -m, --min-shift INT set minimal interval size for CSI indices to 2^INT [14]\n"); fprintf(stderr, " -t, --tbi generate TBI-format index for VCF files\n"); fprintf(stderr, "\n"); fprintf(stderr, "Stats options:\n"); fprintf(stderr, " -n, --nrecords print number of records based on existing index file\n"); fprintf(stderr, " -s, --stats print per contig stats based on existing index file\n"); fprintf(stderr, "\n"); exit(1); } int vcf_index_stats(char *fname, int stats) { char *fn_out = NULL; FILE *out; out = fn_out ? fopen(fn_out, "w") : stdout; const char **seq; int i, nseq; tbx_t *tbx = NULL; hts_idx_t *idx = NULL; htsFile *fp = hts_open(fname,"r"); if ( !fp ) { fprintf(stderr,"Could not read %s\n", fname); return 1; } bcf_hdr_t *hdr = bcf_hdr_read(fp); if ( !hdr ) { fprintf(stderr,"Could not read the header: %s\n", fname); return 1; } if ( hts_get_format(fp)->format==vcf ) { tbx = tbx_index_load(fname); if ( !tbx ) { fprintf(stderr,"Could not load TBI index: %s\n", fname); return 1; } } else if ( hts_get_format(fp)->format==bcf ) { idx = bcf_index_load(fname); if ( !idx ) { fprintf(stderr,"Could not load CSI index: %s\n", fname); return 1; } } else { fprintf(stderr,"Could not detect the file type as VCF or BCF: %s\n", fname); return 1; } seq = tbx ? tbx_seqnames(tbx, &nseq) : bcf_index_seqnames(idx, hdr, &nseq); uint64_t sum = 0; for (i=0; iidx : idx, i, &records, &v); sum+=records; if (stats&2 || !records) continue; bcf_hrec_t *hrec = bcf_hdr_get_hrec(hdr, BCF_HL_CTG, "ID", seq[i], NULL); int hkey = hrec ? bcf_hrec_find_key(hrec, "length") : -1; if (hkey<0) { fprintf(stderr,"could not get contig length for %s\n", seq[i]); return 1; } fprintf(out, "%s\t%s", seq[i], strcmp(hrec->vals[hkey], "2147483647")==0 ? "." : hrec->vals[hkey]); fprintf(out, "\t%" PRIu64 "\n", records); } if (!sum) { // No counts found. // Is this because index version has no stored count data, or no records? bcf1_t *rec = bcf_init1(); if (bcf_read1(fp, hdr, rec) >= 0) { fprintf(stderr,"%s index of %s does not contain any count metadata. Please re-index with a newer version of bcftools or tabix.\n", tbx ? "TBI" : "CSI", fname); return 1; } bcf_destroy1(rec); } if (stats&2) fprintf(out, "%" PRIu64 "\n", sum); free(seq); fclose(out); hts_close(fp); bcf_hdr_destroy(hdr); if (tbx) tbx_destroy(tbx); if (idx) hts_idx_destroy(idx); return 0; } int main_vcfindex(int argc, char *argv[]) { int c, force = 0, tbi = 0, stats = 0; int min_shift = BCF_LIDX_SHIFT; static struct option loptions[] = { {"csi",no_argument,NULL,'c'}, {"tbi",no_argument,NULL,'t'}, {"force",no_argument,NULL,'f'}, {"min-shift",required_argument,NULL,'m'}, {"stats",no_argument,NULL,'s'}, {"nrecords",no_argument,NULL,'n'}, {NULL, 0, NULL, 0} }; char *tmp; while ((c = getopt_long(argc, argv, "ctfm:sn", loptions, NULL)) >= 0) { switch (c) { case 'c': tbi = 0; break; case 't': tbi = 1; min_shift = 0; break; case 'f': force = 1; break; case 'm': min_shift = strtol(optarg,&tmp,10); if ( *tmp ) error("Could not parse argument: --min-shift %s\n", optarg); break; case 's': stats |= 1; break; case 'n': stats |= 2; break; default: usage(); } } if ( optind==argc ) usage(); if (stats>2) { fprintf(stderr, "[E::%s] expected only one of --stats or --nrecords options\n", __func__); return 1; } if (tbi && min_shift>0) { fprintf(stderr, "[E::%s] min-shift option only expected for CSI indices \n", __func__); return 1; } if (min_shift < 0 || min_shift > 30) { fprintf(stderr, "[E::%s] expected min_shift in range [0,30] (%d)\n", __func__, min_shift); return 1; } char *fname = argv[optind]; if (stats) return vcf_index_stats(fname, stats); htsFile *fp = hts_open(fname,"r"); htsFormat type = *hts_get_format(fp); hts_close(fp); if ( (type.format!=bcf && type.format!=vcf) || type.compression!=bgzf ) { fprintf(stderr, "[E::%s] unknown filetype; expected bgzip compressed VCF or BCF\n", __func__); if ( type.compression!=bgzf ) fprintf(stderr, "[E::%s] was the VCF/BCF compressed with bgzip?\n", __func__); return 1; } if (tbi && type.format==bcf) { fprintf(stderr, "[Warning] TBI-index does not work for BCF files. Generating CSI instead.\n"); tbi = 0; min_shift = BCF_LIDX_SHIFT; } if (min_shift == 0 && type.format==bcf) { fprintf(stderr, "[E::%s] Require min_shift>0 for BCF files.\n", __func__); return 1; } if (!tbi && type.format==vcf && min_shift == 0) { fprintf(stderr, "[Warning] min-shift set to 0 for VCF file. Generating TBI file.\n"); tbi = 1; } if (!force) { // Before complaining about existing index, check if the VCF file isn't newer. char *idx_fname = (char*)alloca(strlen(fname) + 5); strcat(strcpy(idx_fname, fname), tbi ? ".tbi" : ".csi"); struct stat stat_tbi, stat_file; if ( stat(idx_fname, &stat_tbi)==0 ) { stat(fname, &stat_file); if ( stat_file.st_mtime <= stat_tbi.st_mtime ) { fprintf(stderr,"[E::%s] the index file exists. Please use '-f' to overwrite.\n", __func__); return 1; } } } if (type.format==bcf) { if ( bcf_index_build(fname, min_shift) != 0 ) { fprintf(stderr,"[E::%s] bcf_index_build failed for %s\n", __func__, fname); return 1; } } else { if ( tbx_index_build(fname, min_shift, &tbx_conf_vcf) != 0 ) { fprintf(stderr,"[E::%s] tbx_index_build failed for %s\n", __func__, fname); return 1; } } return 0; } bcftools-1.2/vcfisec.c000066400000000000000000000557721246371514100150010ustar00rootroot00000000000000/* vcfisec.c -- Create intersections, unions and complements of VCF files. Copyright (C) 2012-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include #include #include "bcftools.h" #include "filter.h" #define OP_PLUS 1 #define OP_MINUS 2 #define OP_EQUAL 3 #define OP_VENN 4 #define OP_COMPLEMENT 5 // Logic of the filters: include or exclude sites which match the filters? #define FLT_INCLUDE 1 #define FLT_EXCLUDE 2 typedef struct { int isec_op, isec_n, *write, iwrite, nwrite, output_type; int nflt, *flt_logic; filter_t **flt; char **flt_expr; bcf_srs_t *files; FILE *fh_log, *fh_sites; htsFile **fh_out; char **argv, *prefix, *output_fname, **fnames, *write_files, *targets_list, *regions_list; int argc; } args_t; /** * mkdir_p() - create new directory for a file $fname * @fname: the file name to create the directory for, the part after last "/" is ignored */ void mkdir_p(const char *fmt, ...) { va_list ap; va_start(ap, fmt); int n = vsnprintf(NULL, 0, fmt, ap) + 2; va_end(ap); char *path = (char*)malloc(n); va_start(ap, fmt); vsnprintf(path, n, fmt, ap); va_end(ap); char *tmp = strdup(path), *p = tmp+1; while (*p) { while (*p && *p!='/') p++; if ( *p ) { *p = 0; mkdir(tmp,S_IRWXU | S_IRWXG | S_IROTH | S_IXOTH); *p = '/'; p++; } } free(tmp); free(path); } /** * open_file() - open new file creating the file name using vsnprintf * @fname: if not NULL, on output will point to newly allocated fname string * @mode: if NULL, only the file name string will be created * @fmt: vsnprintf format and args * * Returns open file descriptor or NULL if mode is NULL. */ FILE *open_file(char **fname, const char *mode, const char *fmt, ...) { va_list ap; va_start(ap, fmt); int n = vsnprintf(NULL, 0, fmt, ap) + 2; va_end(ap); char *str = (char*)malloc(n); va_start(ap, fmt); vsnprintf(str, n, fmt, ap); va_end(ap); mkdir_p(str); if ( !mode ) { if ( !fname ) error("Uh: expected fname or mode\n"); *fname = str; return NULL; } FILE *fp = fopen(str,mode); if ( fname ) *fname = str; else free(str); return fp; } const char *hts_bcf_wmode(int file_type) { if ( file_type == FT_BCF ) return "wbu"; // uncompressed BCF if ( file_type & FT_BCF ) return "wb"; // compressed BCF if ( file_type & FT_GZ ) return "wz"; // compressed VCF return "w"; // uncompressed VCF } void isec_vcf(args_t *args) { bcf_srs_t *files = args->files; kstring_t str = {0,0,0}; htsFile *out_fh = NULL; // When only one VCF is output, print VCF to stdout or -o file int out_std = 0; if ( args->nwrite==1 && !args->prefix ) out_std = 1; if ( args->targets_list && files->nreaders==1 ) out_std = 1; if ( out_std ) { out_fh = hts_open(args->output_fname? args->output_fname : "-",hts_bcf_wmode(args->output_type)); if ( out_fh == NULL ) error("Can't write to %s: %s\n", args->output_fname? args->output_fname : "standard output", strerror(errno)); bcf_hdr_append_version(files->readers[args->iwrite].header,args->argc,args->argv,"bcftools_isec"); bcf_hdr_write(out_fh, files->readers[args->iwrite].header); } if ( !args->nwrite && !out_std && !args->prefix ) fprintf(stderr,"Note: -w option not given, printing list of sites...\n"); int n; while ( (n=bcf_sr_next_line(files)) ) { bcf_sr_t *reader = NULL; bcf1_t *line = NULL; int i, ret = 0; for (i=0; inreaders; i++) { if ( !bcf_sr_has_line(files,i) ) continue; if ( args->nflt && args->flt[i] ) { bcf1_t *rec = bcf_sr_get_line(files, i); int pass = filter_test(args->flt[i], rec, NULL); if ( args->flt_logic[i] & FLT_EXCLUDE ) pass = pass ? 0 : 1; if ( !pass ) { files->has_line[i] = 0; n--; continue; } } if ( !line ) { line = files->readers[i].buffer[0]; reader = &files->readers[i]; } ret |= 1<isec_op) { case OP_COMPLEMENT: if ( n!=1 || !bcf_sr_has_line(files,0) ) continue; break; case OP_EQUAL: if ( n != args->isec_n ) continue; break; case OP_PLUS: if ( n < args->isec_n ) continue; break; case OP_MINUS: if ( n > args->isec_n ) continue; } if ( out_std ) { if ( bcf_sr_has_line(files,args->iwrite) ) bcf_write1(out_fh, files->readers[args->iwrite].header, files->readers[args->iwrite].buffer[0]); continue; } else if ( args->fh_sites ) { str.l = 0; kputs(reader->header->id[BCF_DT_CTG][line->rid].key, &str); kputc('\t', &str); kputw(line->pos+1, &str); kputc('\t', &str); if (line->n_allele > 0) kputs(line->d.allele[0], &str); else kputc('.', &str); kputc('\t', &str); if (line->n_allele > 1) kputs(line->d.allele[1], &str); else kputc('.', &str); for (i=2; in_allele; i++) { kputc(',', &str); kputs(line->d.allele[i], &str); } kputc('\t', &str); for (i=0; inreaders; i++) kputc(bcf_sr_has_line(files,i)?'1':'0', &str); kputc('\n', &str); fwrite(str.s,sizeof(char),str.l,args->fh_sites); } if ( args->prefix ) { if ( args->isec_op==OP_VENN && ret==3 ) { if ( !args->nwrite || args->write[0] ) bcf_write1(args->fh_out[2], bcf_sr_get_header(files,0), bcf_sr_get_line(files,0)); if ( !args->nwrite || args->write[1] ) bcf_write1(args->fh_out[3], bcf_sr_get_header(files,1), bcf_sr_get_line(files,1)); } else { for (i=0; inreaders; i++) { if ( !bcf_sr_has_line(files,i) ) continue; if ( args->write && !args->write[i] ) continue; bcf_write1(args->fh_out[i], files->readers[i].header, files->readers[i].buffer[0]); } } } } if ( str.s ) free(str.s); if ( out_fh ) hts_close(out_fh); } static void add_filter(args_t *args, char *expr, int logic) { args->nflt++; args->flt_expr = (char**) realloc(args->flt_expr,sizeof(char*)*args->nflt); args->flt_logic = (int*) realloc(args->flt_logic,sizeof(int)*args->nflt); args->flt = (filter_t**) realloc(args->flt,sizeof(filter_t*)*args->nflt); if ( expr[0]=='-' && expr[1]==0 ) { args->flt_expr[args->nflt-1] = NULL; args->flt[args->nflt-1] = NULL; } else args->flt_expr[args->nflt-1] = expr; args->flt_logic[args->nflt-1] = logic; } static void destroy_data(args_t *args); static void init_data(args_t *args) { int i; if ( args->nflt ) { if ( args->nflt > 1 && args->nflt!=args->files->nreaders ) error("Error: expected either one -i/-e option or as many as there are input files\n"); if ( args->nflt < args->files->nreaders ) { if ( !args->flt_expr[0] ) error("Error: useless use of -i/-e\n"); args->nflt = args->files->nreaders; args->flt_expr = (char**) realloc(args->flt_expr,sizeof(char*)*args->nflt); args->flt_logic = (int*) realloc(args->flt_logic,sizeof(int)*args->nflt); args->flt = (filter_t**) realloc(args->flt,sizeof(filter_t*)*args->nflt); for (i=1; inflt; i++) { args->flt_expr[i] = args->flt_expr[0]; args->flt_logic[i] = args->flt_logic[0]; args->flt[i] = filter_init(args->files->readers[i].header,args->flt_expr[i]); } args->flt[0] = filter_init(args->files->readers[0].header,args->flt_expr[0]); } else { for (i=0; ifiles->nreaders; i++) { if ( !args->flt_expr[i] ) continue; args->flt[i] = filter_init(args->files->readers[i].header,args->flt_expr[i]); } } } // Which files to write: parse the string passed with -w char *p = args->write_files; while (p && *p) { if ( !args->write ) args->write = (int*) calloc(args->files->nreaders,sizeof(int)); if ( sscanf(p,"%d",&i)!=1 ) error("Could not parse --write %s\n", args->write_files); if ( i<0 || i>args->files->nreaders ) error("The index is out of range: %d (%s)\n", i, args->write_files); args->write[i-1] = 1; args->iwrite = i-1; args->nwrite++; while (*p && *p!=',') p++; if ( *p==',' ) p++; } if ( args->nwrite>1 && !args->prefix ) error("Expected -p when mutliple output files given: --write %s\n", args->write_files); if ( args->isec_op==OP_COMPLEMENT && args->nwrite ) { if ( args->nwrite>1 ) error("Multiple files to -w make no sense with -C\n"); if ( !args->write[0] ) error("Only -w1 makes sense with -C\n"); } if ( args->prefix ) { // Init output directory and create the readme file args->fh_log = open_file(NULL,"w","%s/README.txt", args->prefix); if ( !args->fh_log ) error("%s/README.txt: %s\n", args->prefix, strerror(errno)); fprintf(args->fh_log,"This file was produced by vcfisec.\n"); fprintf(args->fh_log,"The command line was:\tbcftools %s ", args->argv[0]); int i; for (i=1; iargc; i++) fprintf(args->fh_log," %s",args->argv[i]); fprintf(args->fh_log,"\n\nUsing the following file names:\n"); const char *suffix = "vcf"; if ( args->output_type & FT_BCF ) suffix = "bcf"; else if ( args->output_type & FT_GZ ) suffix = "vcf.gz"; // Open output files and write the legend if ( args->isec_op==OP_VENN ) { args->fh_out = (htsFile**) malloc(sizeof(htsFile*)*4); args->fnames = (char**) calloc(4,sizeof(char*)); #define OPEN_FILE(i,j) { \ open_file(&args->fnames[i], NULL, "%s/%04d.%s", args->prefix, i, suffix); \ args->fh_out[i] = hts_open(args->fnames[i], hts_bcf_wmode(args->output_type)); \ if ( !args->fh_out[i] ) error("Could not open %s\n", args->fnames[i]); \ bcf_hdr_append_version(args->files->readers[j].header,args->argc,args->argv,"bcftools_isec"); \ bcf_hdr_write(args->fh_out[i], args->files->readers[j].header); \ } if ( !args->nwrite || args->write[0] ) { OPEN_FILE(0,0); fprintf(args->fh_log,"%s\tfor records private to\t%s\n", args->fnames[0], args->files->readers[0].fname); } if ( !args->nwrite || args->write[1] ) { OPEN_FILE(1,1); fprintf(args->fh_log,"%s\tfor records private to\t%s\n", args->fnames[1], args->files->readers[1].fname); } if ( !args->nwrite || args->write[0] ) { OPEN_FILE(2,0); fprintf(args->fh_log,"%s\tfor records from %s shared by both\t%s %s\n", args->fnames[2], args->files->readers[0].fname, args->files->readers[0].fname, args->files->readers[1].fname); } if ( !args->nwrite || args->write[1] ) { OPEN_FILE(3,1); fprintf(args->fh_log,"%s\tfor records from %s shared by both\t%s %s\n", args->fnames[3], args->files->readers[1].fname, args->files->readers[0].fname, args->files->readers[1].fname); } } else { // Init one output file for each reader args->fh_out = (htsFile**) calloc(args->files->nreaders, sizeof(htsFile*)); args->fnames = (char**) calloc(args->files->nreaders, sizeof(char*)); for (i=0; ifiles->nreaders; i++) { if ( args->write && !args->write[i] ) continue; if ( args->isec_op==OP_COMPLEMENT && i>0 ) break; OPEN_FILE(i,i); fprintf(args->fh_log,"%s\tfor stripped\t%s\n", args->fnames[i], args->files->readers[i].fname); } #undef OPEN_FILE args->fh_sites = open_file(NULL, "w", "%s/sites.txt", args->prefix); if ( !args->fh_sites ) error("%s/sites.txt: %s\n", args->prefix, strerror(errno)); } } else { if (args->output_fname) { args->fh_sites = fopen(args->output_fname, "w"); if ( args->fh_sites == NULL ) error("Can't write to \"%s\": %s\n", args->output_fname, strerror(errno)); } else args->fh_sites = stdout; } } static void destroy_data(args_t *args) { int i; if ( args->nflt ) { for (i=0; inflt; i++) { if ( !args->flt[i] ) continue; filter_destroy(args->flt[i]); } free(args->flt_expr); free(args->flt); free(args->flt_logic); } if ( args->prefix ) { fclose(args->fh_log); int n = args->isec_op==OP_VENN ? 4 : args->files->nreaders; for (i=0; ifnames[i] ) continue; hts_close(args->fh_out[i]); if ( args->output_type==FT_VCF_GZ ) { tbx_conf_t conf = tbx_conf_vcf; tbx_index_build(args->fnames[i], -1, &conf); } else if ( args->output_type==FT_BCF_GZ ) { if ( bcf_index_build(args->fnames[i],14) ) error("Could not index %s\n", args->fnames[i]); } free(args->fnames[i]); } free(args->fh_out); free(args->fnames); if ( args->fh_sites ) fclose(args->fh_sites); if ( args->write ) free(args->write); } } static void usage(void) { fprintf(stderr, "\n"); fprintf(stderr, "About: Create intersections, unions and complements of VCF files.\n"); fprintf(stderr, "Usage: bcftools isec [options] [...]\n"); fprintf(stderr, "\n"); fprintf(stderr, "Options:\n"); fprintf(stderr, " -c, --collapse treat as identical records with , see man page for details [none]\n"); fprintf(stderr, " -C, --complement output positions present only in the first file but missing in the others\n"); fprintf(stderr, " -e, --exclude exclude sites for which the expression is true\n"); fprintf(stderr, " -f, --apply-filters require at least one of the listed FILTER strings (e.g. \"PASS,.\")\n"); fprintf(stderr, " -i, --include include only sites for which the expression is true\n"); fprintf(stderr, " -n, --nfiles [+-=] output positions present in this many (=), this many or more (+), or this many or fewer (-) files\n"); fprintf(stderr, " -o, --output write output to a file [standard output]\n"); fprintf(stderr, " -O, --output-type b: compressed BCF, u: uncompressed BCF, z: compressed VCF, v: uncompressed VCF [v]\n"); fprintf(stderr, " -p, --prefix if given, subset each of the input files accordingly, see also -w\n"); fprintf(stderr, " -r, --regions restrict to comma-separated list of regions\n"); fprintf(stderr, " -R, --regions-file restrict to regions listed in a file\n"); fprintf(stderr, " -t, --targets similar to -r but streams rather than index-jumps\n"); fprintf(stderr, " -T, --targets-file similar to -R but streams rather than index-jumps\n"); fprintf(stderr, " -w, --write list of files to write with -p given as 1-based indexes. By default, all files are written\n"); fprintf(stderr, "\n"); fprintf(stderr, "Examples:\n"); fprintf(stderr, " # Create intersection and complements of two sets saving the output in dir/*\n"); fprintf(stderr, " bcftools isec A.vcf.gz B.vcf.gz -p dir\n"); fprintf(stderr, "\n"); fprintf(stderr, " # Filter sites in A and B (but not in C) and create intersection\n"); fprintf(stderr, " bcftools isec -e'MAF<0.01' -i'dbSNP=1' -e - A.vcf.gz B.vcf.gz C.vcf.gz -p dir\n"); fprintf(stderr, "\n"); fprintf(stderr, " # Extract and write records from A shared by both A and B using exact allele match\n"); fprintf(stderr, " bcftools isec A.vcf.gz B.vcf.gz -p dir -n =2 -w 1\n"); fprintf(stderr, "\n"); fprintf(stderr, " # Extract records private to A or B comparing by position only\n"); fprintf(stderr, " bcftools isec A.vcf.gz B.vcf.gz -p dir -n -1 -c all\n"); fprintf(stderr, "\n"); exit(1); } int main_vcfisec(int argc, char *argv[]) { int c; args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->files = bcf_sr_init(); args->argc = argc; args->argv = argv; args->output_fname = NULL; args->output_type = FT_VCF; int targets_is_file = 0, regions_is_file = 0; static struct option loptions[] = { {"help",0,0,'h'}, {"exclude",1,0,'e'}, {"include",1,0,'i'}, {"collapse",1,0,'c'}, {"complement",0,0,'C'}, {"apply-filters",1,0,'f'}, {"nfiles",1,0,'n'}, {"prefix",1,0,'p'}, {"write",1,0,'w'}, {"targets",1,0,'t'}, {"targets-file",1,0,'T'}, {"regions",1,0,'r'}, {"regions-file",1,0,'R'}, {"output",1,0,'o'}, {"output-type",1,0,'O'}, {0,0,0,0} }; while ((c = getopt_long(argc, argv, "hc:r:R:p:n:w:t:T:Cf:o:O:i:e:",loptions,NULL)) >= 0) { switch (c) { case 'o': args->output_fname = optarg; break; case 'O': switch (optarg[0]) { case 'b': args->output_type = FT_BCF_GZ; break; case 'u': args->output_type = FT_BCF; break; case 'z': args->output_type = FT_VCF_GZ; break; case 'v': args->output_type = FT_VCF; break; default: error("The output type \"%s\" not recognised\n", optarg); } break; case 'c': if ( !strcmp(optarg,"snps") ) args->files->collapse |= COLLAPSE_SNPS; else if ( !strcmp(optarg,"indels") ) args->files->collapse |= COLLAPSE_INDELS; else if ( !strcmp(optarg,"both") ) args->files->collapse |= COLLAPSE_SNPS | COLLAPSE_INDELS; else if ( !strcmp(optarg,"any") ) args->files->collapse |= COLLAPSE_ANY; else if ( !strcmp(optarg,"all") ) args->files->collapse |= COLLAPSE_ANY; else if ( !strcmp(optarg,"some") ) args->files->collapse |= COLLAPSE_SOME; else if ( !strcmp(optarg,"none") ) args->files->collapse = COLLAPSE_NONE; else error("The --collapse string \"%s\" not recognised.\n", optarg); break; case 'f': args->files->apply_filters = optarg; break; case 'C': args->isec_op = OP_COMPLEMENT; break; case 'r': args->regions_list = optarg; break; case 'R': args->regions_list = optarg; regions_is_file = 1; break; case 't': args->targets_list = optarg; break; case 'T': args->targets_list = optarg; targets_is_file = 1; break; case 'p': args->prefix = optarg; break; case 'w': args->write_files = optarg; break; case 'i': add_filter(args, optarg, FLT_INCLUDE); break; case 'e': add_filter(args, optarg, FLT_EXCLUDE); break; case 'n': { char *p = optarg; if ( *p=='-' ) { args->isec_op = OP_MINUS; p++; } else if ( *p=='+' ) { args->isec_op = OP_PLUS; p++; } else if ( *p=='=' ) { args->isec_op = OP_EQUAL; p++; } else if ( isdigit(*p) ) args->isec_op = OP_EQUAL; else error("Could not parse --nfiles %s\n", optarg); if ( sscanf(p,"%d",&args->isec_n)!=1 ) error("Could not parse --nfiles %s\n", optarg); } break; case 'h': case '?': usage(); default: error("Unknown argument: %s\n", optarg); } } if ( argc-optind<1 ) usage(); // no file given if ( args->targets_list && bcf_sr_set_targets(args->files, args->targets_list, targets_is_file,0)<0 ) error("Failed to read the targets: %s\n", args->targets_list); if ( args->regions_list && bcf_sr_set_regions(args->files, args->regions_list, regions_is_file)<0 ) error("Failed to read the regions: %s\n", args->regions_list); if ( argc-optind==2 && !args->isec_op ) { args->isec_op = OP_VENN; if ( !args->prefix ) error("Expected the -p option\n"); } if ( !args->targets_list ) { if ( argc-optind<2 ) error("Expected multiple files or the --targets option\n"); if ( !args->isec_op ) error("Expected two file names or one of the options --complement, --nfiles or --targets\n"); } args->files->require_index = 1; while (optindfiles, argv[optind]) ) error("Failed to open %s: %s\n", argv[optind],bcf_sr_strerror(args->files->errnum)); optind++; } init_data(args); isec_vcf(args); destroy_data(args); bcf_sr_destroy(args->files); free(args); return 0; } bcftools-1.2/vcfmerge.c000066400000000000000000002342431246371514100151450ustar00rootroot00000000000000/* vcfmerge.c -- Merge multiple VCF/BCF files to create one multi-sample file. Copyright (C) 2012-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include #include "bcftools.h" #include "vcmp.h" #include KHASH_MAP_INIT_STR(strdict, int) typedef khash_t(strdict) strdict_t; #define SKIP_DONE 1 #define SKIP_DIFF 2 #define IS_VL_G(hdr,id) (bcf_hdr_id2length(hdr,BCF_HL_FMT,id) == BCF_VL_G) #define IS_VL_A(hdr,id) (bcf_hdr_id2length(hdr,BCF_HL_FMT,id) == BCF_VL_A) #define IS_VL_R(hdr,id) (bcf_hdr_id2length(hdr,BCF_HL_FMT,id) == BCF_VL_R) // For merging INFO Number=A,G,R tags typedef struct { const char *hdr_tag; int type, nvals; int nbuf, mbuf; uint8_t *buf; } AGR_info_t; // Rules for merging arbitrary INFO tags typedef struct _info_rule_t { char *hdr_tag; void (*merger)(bcf_hdr_t *hdr, bcf1_t *line, struct _info_rule_t *rule); int type; // one of BCF_HT_* int block_size; // number of values in a block int nblocks; // number of blocks in nvals (the number of merged files) int nvals, mvals; // used and total size of vals array void *vals; // the info tag values } info_rule_t; // Auxiliary merge data for selecting the right combination // of buffered records across multiple readers. maux1_t // corresponds to one buffered line. typedef struct { int skip; int *map; // mapping from input alleles to the output array int mmap; // size of map array (only buffer[i].n_allele is actually used) int als_differ; } maux1_t; typedef struct { int n; // number of readers char **als, **out_als; // merged alleles (temp, may contain empty records) and merged alleles ready for output int nals, mals, nout_als, mout_als; // size of the output array int *cnt, ncnt; // number of records that refer to the alleles int *nbuf; // readers have buffers of varying lengths int *smpl_ploidy, *smpl_nGsize; // ploidy and derived number of values in Number=G tags, updated for each line (todo: cache for missing cases) int *flt, mflt, minf; bcf_info_t *inf;// out_line's INFO fields bcf_fmt_t **fmt_map; // i-th output FORMAT field corresponds in j-th reader to i*nreader+j, first row is reserved for GT int nfmt_map; // number of rows in the fmt_map array int *agr_map, nagr_map, magr_map; // mapping between Number=AGR element indexes void *tmp_arr; int ntmp_arr; maux1_t **d; // d[i][j] i-th reader, j-th buffer line AGR_info_t *AGR_info; int nAGR_info, mAGR_info; bcf_srs_t *files; int *has_line; // which files are being merged } maux_t; typedef struct { vcmp_t *vcmp; maux_t *maux; int header_only, collapse, output_type, force_samples, merge_by_id; char *header_fname, *output_fname, *regions_list, *info_rules, *file_list; info_rule_t *rules; int nrules; strdict_t *tmph; kstring_t tmps; bcf_srs_t *files; bcf1_t *out_line; htsFile *out_fh; bcf_hdr_t *out_hdr; char **argv; int argc; } args_t; static void info_rules_merge_sum(bcf_hdr_t *hdr, bcf1_t *line, info_rule_t *rule) { if ( !rule->nvals ) return; int i, j, ndim = rule->block_size; #define BRANCH(type_t,is_missing) { \ type_t *ptr = (type_t*) rule->vals; \ for (i=0; invals; i++) if ( is_missing ) ptr[i] = 0; \ for (i=1; inblocks; i++) \ { \ for (j=0; jtype) { case BCF_HT_INT: BRANCH(int32_t, ptr[i]==bcf_int32_missing); break; case BCF_HT_REAL: BRANCH(float, bcf_float_is_missing(ptr[i])); break; default: error("TODO: %s:%d .. type=%d\n", __FILE__,__LINE__, rule->type); } #undef BRANCH bcf_update_info(hdr,line,rule->hdr_tag,rule->vals,ndim,rule->type); } static void info_rules_merge_avg(bcf_hdr_t *hdr, bcf1_t *line, info_rule_t *rule) { if ( !rule->nvals ) return; int i, j, ndim = rule->block_size; #define BRANCH(type_t,is_missing) { \ type_t *ptr = (type_t*) rule->vals; \ for (i=0; invals; i++) if ( is_missing ) ptr[i] = 0; \ for (j=0; jnblocks; i++) sum += ptr[j+i*ndim]; \ ptr[j] = sum / rule->nblocks; \ } \ } switch (rule->type) { case BCF_HT_INT: BRANCH(int32_t, ptr[i]==bcf_int32_missing); break; case BCF_HT_REAL: BRANCH(float, bcf_float_is_missing(ptr[i])); break; default: error("TODO: %s:%d .. type=%d\n", __FILE__,__LINE__, rule->type); } #undef BRANCH bcf_update_info(hdr,line,rule->hdr_tag,rule->vals,ndim,rule->type); } static void info_rules_merge_min(bcf_hdr_t *hdr, bcf1_t *line, info_rule_t *rule) { if ( !rule->nvals ) return; int i, j, ndim = rule->block_size; #define BRANCH(type_t,is_missing,set_missing,huge_val) { \ type_t *ptr = (type_t*) rule->vals; \ for (i=0; invals; i++) if ( is_missing ) ptr[i] = huge_val; \ for (i=1; inblocks; i++) \ { \ for (j=0; j ptr[j+i*ndim] ) ptr[j] = ptr[j+i*ndim]; \ } \ for (i=0; invals; i++) if ( ptr[i]==huge_val ) set_missing; \ } switch (rule->type) { case BCF_HT_INT: BRANCH(int32_t, ptr[i]==bcf_int32_missing, ptr[i]=bcf_int32_missing, INT32_MAX); break; case BCF_HT_REAL: BRANCH(float, bcf_float_is_missing(ptr[i]), bcf_float_set_missing(ptr[i]), HUGE_VAL); break; default: error("TODO: %s:%d .. type=%d\n", __FILE__,__LINE__, rule->type); } #undef BRANCH bcf_update_info(hdr,line,rule->hdr_tag,rule->vals,ndim,rule->type); } static void info_rules_merge_max(bcf_hdr_t *hdr, bcf1_t *line, info_rule_t *rule) { if ( !rule->nvals ) return; int i, j, ndim = rule->block_size; #define BRANCH(type_t,is_missing,set_missing,huge_val) { \ type_t *ptr = (type_t*) rule->vals; \ for (i=0; invals; i++) if ( is_missing ) ptr[i] = huge_val; \ for (i=1; inblocks; i++) \ { \ for (j=0; jnvals; i++) if ( ptr[i]==huge_val ) set_missing; \ } switch (rule->type) { case BCF_HT_INT: BRANCH(int32_t, ptr[i]==bcf_int32_missing, ptr[i]=bcf_int32_missing, INT32_MIN); break; case BCF_HT_REAL: BRANCH(float, bcf_float_is_missing(ptr[i]), bcf_float_set_missing(ptr[i]), -HUGE_VAL); break; default: error("TODO: %s:%d .. type=%d\n", __FILE__,__LINE__, rule->type); } #undef BRANCH bcf_update_info(hdr,line,rule->hdr_tag,rule->vals,ndim,rule->type); } static void info_rules_merge_join(bcf_hdr_t *hdr, bcf1_t *line, info_rule_t *rule) { if ( !rule->nvals ) return; if ( rule->type==BCF_HT_STR ) { ((char*)rule->vals)[rule->nvals] = 0; bcf_update_info_string(hdr,line,rule->hdr_tag,rule->vals); } else bcf_update_info(hdr,line,rule->hdr_tag,rule->vals,rule->nvals,rule->type); } static int info_rules_comp_key2(const void *a, const void *b) { info_rule_t *rule1 = (info_rule_t*) a; info_rule_t *rule2 = (info_rule_t*) b; return strcmp(rule1->hdr_tag, rule2->hdr_tag); } static int info_rules_comp_key(const void *a, const void *b) { char *key = (char*) a; info_rule_t *rule = (info_rule_t*) b; return strcmp(key, rule->hdr_tag); } static void info_rules_init(args_t *args) { if ( args->info_rules && !strcmp("-",args->info_rules) ) return; kstring_t str = {0,0,0}; if ( !args->info_rules ) { if ( bcf_hdr_idinfo_exists(args->out_hdr,BCF_HL_INFO,bcf_hdr_id2int(args->out_hdr, BCF_DT_ID, "DP")) ) kputs("DP:sum",&str); if ( bcf_hdr_idinfo_exists(args->out_hdr,BCF_HL_INFO,bcf_hdr_id2int(args->out_hdr, BCF_DT_ID, "DP4")) ) { if ( str.l ) kputc(',',&str); kputs("DP4:sum",&str); } if ( !str.l ) return; args->info_rules = str.s; } args->nrules = 1; char *ss = strdup(args->info_rules), *tmp = ss; int n = 0; while ( *ss ) { if ( *ss==':' ) { *ss = 0; n++; if ( n%2==0 ) error("Could not parse INFO rules: \"%s\"\n", args->info_rules); } else if ( *ss==',' ) { *ss = 0; args->nrules++; n++; if ( n%2==1 ) error("Could not parse INFO rules: \"%s\"\n", args->info_rules); } ss++; } if ( n%2==0 ) error("Could not parse INFO rules: \"%s\"\n", args->info_rules); args->rules = (info_rule_t*) calloc(args->nrules,sizeof(info_rule_t)); n = 0; ss = tmp; while ( n < args->nrules ) { info_rule_t *rule = &args->rules[n]; rule->hdr_tag = strdup(ss); int id = bcf_hdr_id2int(args->out_hdr, BCF_DT_ID, rule->hdr_tag); if ( !bcf_hdr_idinfo_exists(args->out_hdr,BCF_HL_INFO,id) ) error("The tag is not defined in the header: \"%s\"\n", rule->hdr_tag); rule->type = bcf_hdr_id2type(args->out_hdr,BCF_HL_INFO,id); if ( rule->type!=BCF_HT_INT && rule->type!=BCF_HT_REAL && rule->type!=BCF_HT_STR ) error("The type is not supported: \"%s\"\n", rule->hdr_tag); while ( *ss ) ss++; ss++; if ( !*ss ) error("Could not parse INFO rules, missing logic of \"%s\"\n", rule->hdr_tag); int is_join = 0; if ( !strcasecmp(ss,"sum") ) rule->merger = info_rules_merge_sum; else if ( !strcasecmp(ss,"avg") ) rule->merger = info_rules_merge_avg; else if ( !strcasecmp(ss,"min") ) rule->merger = info_rules_merge_min; else if ( !strcasecmp(ss,"max") ) rule->merger = info_rules_merge_max; else if ( !strcasecmp(ss,"join") ) { rule->merger = info_rules_merge_join; is_join = 1; } else error("The rule logic \"%s\" not recognised\n", ss); if ( !is_join && rule->type==BCF_HT_STR ) error("Numeric operation \"%s\" requested on non-numeric field: %s\n", ss, rule->hdr_tag); if ( bcf_hdr_id2number(args->out_hdr,BCF_HL_INFO,id)==0xfffff ) { int is_agr = ( bcf_hdr_id2length(args->out_hdr,BCF_HL_INFO,id)==BCF_VL_A || bcf_hdr_id2length(args->out_hdr,BCF_HL_INFO,id)==BCF_VL_G || bcf_hdr_id2length(args->out_hdr,BCF_HL_INFO,id)==BCF_VL_R ) ? 1 : 0; if ( is_join && is_agr ) error("Cannot -i %s:join on Number=[AGR] tags is not supported.\n", rule->hdr_tag); if ( !is_join && !is_agr ) error("Only fixed-length vectors are supported with -i %s:%s\n", ss, rule->hdr_tag); } while ( *ss ) ss++; ss++; n++; } free(str.s); free(tmp); qsort(args->rules, args->nrules, sizeof(*args->rules), info_rules_comp_key2); } static void info_rules_destroy(args_t *args) { int i; for (i=0; inrules; i++) { info_rule_t *rule = &args->rules[i]; free(rule->hdr_tag); free(rule->vals); } free(args->rules); } static void info_rules_reset(args_t *args) { int i; for (i=0; inrules; i++) args->rules[i].nblocks = args->rules[i].nvals = args->rules[i].block_size = 0; } static int info_rules_add_values(args_t *args, bcf_hdr_t *hdr, bcf1_t *line, info_rule_t *rule, maux1_t *als, int var_len) { int ret = bcf_get_info_values(hdr, line, rule->hdr_tag, &args->maux->tmp_arr, &args->maux->ntmp_arr, rule->type); if ( ret<=0 ) error("FIXME: error parsing %s at %s:%d .. %d\n", rule->hdr_tag,bcf_seqname(hdr,line),line->pos+1,ret); rule->nblocks++; if ( rule->type==BCF_HT_STR ) { int need_comma = rule->nblocks==1 ? 0 : 1; hts_expand(char,rule->nvals+ret+need_comma+1,rule->mvals,rule->vals); // 1 for null-termination char *tmp = (char*) rule->vals + rule->nvals; if ( rule->nvals>0 ) { *tmp = ','; tmp++; } strncpy(tmp,(char*)args->maux->tmp_arr,ret); rule->nvals += ret + need_comma; return 1; } int i, j; if ( var_len==BCF_VL_A ) { assert( ret==line->n_allele-1 ); args->maux->nagr_map = ret; hts_expand(int,args->maux->nagr_map,args->maux->magr_map,args->maux->agr_map); // create mapping from source file ALT indexes to dst file indexes for (i=0; imaux->agr_map[i] = als->map[i+1] - 1; rule->block_size = args->maux->nout_als - 1; } else if ( var_len==BCF_VL_R ) { assert( ret==line->n_allele ); args->maux->nagr_map = ret; hts_expand(int,args->maux->nagr_map,args->maux->magr_map,args->maux->agr_map); for (i=0; imaux->agr_map[i] = als->map[i]; rule->block_size = args->maux->nout_als; } else if ( var_len==BCF_VL_G ) { args->maux->nagr_map = bcf_alleles2gt(line->n_allele-1,line->n_allele-1)+1; assert( ret==line->n_allele || ret==args->maux->nagr_map ); if ( ret==line->n_allele ) // haploid { args->maux->nagr_map = line->n_allele; hts_expand(int,args->maux->nagr_map,args->maux->magr_map,args->maux->agr_map); for (i=0; imaux->agr_map[i] = als->map[i]; rule->block_size = args->maux->nout_als; } else { hts_expand(int,args->maux->nagr_map,args->maux->magr_map,args->maux->agr_map); int k_src = 0; for (i=0; in_allele; i++) { for (j=0; j<=i; j++) { args->maux->agr_map[k_src] = bcf_alleles2gt(als->map[i],als->map[j]); k_src++; } } rule->block_size = bcf_alleles2gt(args->maux->nout_als-1,args->maux->nout_als-1)+1; } } else { if ( rule->nblocks>1 && ret!=rule->block_size ) error("Mismatch in number of values for INFO/%s at %s:%d\n", rule->hdr_tag,bcf_seqname(hdr,line),line->pos+1); rule->block_size = ret; args->maux->nagr_map = 0; } #define BRANCH(src_type_t,dst_type_t,set_missing) { \ src_type_t *src = (src_type_t *) args->maux->tmp_arr; \ hts_expand0(dst_type_t,(rule->nvals+rule->block_size),rule->mvals,rule->vals); \ dst_type_t *dst = (dst_type_t *) rule->vals + rule->nvals; \ rule->nvals += rule->block_size; \ if ( !args->maux->nagr_map ) \ { \ for (i=0; iblock_size; i++) set_missing; \ for (i=0; imaux->agr_map[i]] = src[i]; \ } \ } switch (rule->type) { case BCF_HT_INT: BRANCH(int, int32_t, dst[i] = bcf_int32_missing); break; case BCF_HT_REAL: BRANCH(float, float, bcf_float_set_missing(dst[i])); break; default: error("TODO: %s:%d .. type=%d\n", __FILE__,__LINE__, rule->type); } #undef BRANCH return 1; } int bcf_hdr_sync(bcf_hdr_t *h); void bcf_hdr_merge(bcf_hdr_t *hw, const bcf_hdr_t *hr, const char *clash_prefix, int force_samples) { // header lines int ret = bcf_hdr_combine(hw, hr); if ( ret!=0 ) error("Error occurred while merging the headers.\n"); // samples int i; for (i=0; isamples[i]; if ( bcf_hdr_id2int(hw, BCF_DT_SAMPLE, name)!=-1 ) { // there is a sample with the same name if ( !force_samples ) error("Error: Duplicate sample names (%s), use --force-samples to proceed anyway.\n", name); int len = strlen(hr->samples[i]) + strlen(clash_prefix) + 1; name = (char*) malloc(sizeof(char)*(len+1)); sprintf(name,"%s:%s",clash_prefix,hr->samples[i]); bcf_hdr_add_sample(hw,name); free(name); } else bcf_hdr_add_sample(hw,name); } } void debug_als(char **als, int nals) { int k; for (k=0; k=lens[j] ) done = 1; if ( als[j][lens[j]-i] != als[0][lens[0]-i] ) { done = 1; break; } } if ( done ) break; i++; } if ( i>1 ) { i--; als[0][lens[0]-i] = 0; for (j=1; j0, * 1->2, and 2->3. */ char **merge_alleles(char **a, int na, int *map, char **b, int *nb, int *mb) { // reference allele never changes map[0] = 0; int i,j; int rla = !a[0][1] ? 1 : strlen(a[0]); int rlb = !b[0][1] ? 1 : strlen(b[0]); // the most common case: same SNPs if ( na==2 && *nb==2 && rla==1 && rlb==1 && a[1][0]==b[1][0] && !a[1][1] && !b[1][1] ) { map[1] = 1; return b; } // Sanity check: reference prefixes must be identical if ( strncmp(a[0],b[0],rlarlb ) { for (i=0; i<*nb; i++) { int l = strlen(b[i]); b[i] = (char*) realloc(b[i],l+rla-rlb+1); memcpy(b[i]+l,a[0]+rlb,rla-rlb+1); } } // now check if the $a alleles are present and if not add them for (i=1; irla ) // $a alleles need expanding { int l = strlen(a[i]); ai = (char*) malloc(l+rlb-rla+1); memcpy(ai,a[i],l); memcpy(ai+l,b[0]+rla,rlb-rla+1); } else ai = a[i]; for (j=1; j<*nb; j++) if ( !strcmp(ai,b[j]) ) break; if ( j<*nb ) // $b already has the same allele { map[i] = j; if ( rlb>rla ) free(ai); continue; } // new allele map[i] = *nb; b[*nb] = rlb>rla ? ai : strdup(ai); (*nb)++; } return b; } maux_t *maux_init(bcf_srs_t *files) { maux_t *ma = (maux_t*) calloc(1,sizeof(maux_t)); ma->n = files->nreaders; ma->nbuf = (int *) calloc(ma->n,sizeof(int)); ma->d = (maux1_t**) calloc(ma->n,sizeof(maux1_t*)); ma->files = files; int i, n_smpl = 0; for (i=0; in; i++) n_smpl += bcf_hdr_nsamples(files->readers[i].header); ma->smpl_ploidy = (int*) calloc(n_smpl,sizeof(int)); ma->smpl_nGsize = (int*) malloc(n_smpl*sizeof(int)); ma->has_line = (int*) malloc(ma->n*sizeof(int)); return ma; } void maux_destroy(maux_t *ma) { int i; for (i=0; in; i++) // for each reader { if ( !ma->d[i] ) continue; int j; for (j=0; jnbuf[i]; j++) // for each buffered line if ( ma->d[i][j].map ) free(ma->d[i][j].map); free(ma->d[i]); } for (i=0; imAGR_info; i++) free(ma->AGR_info[i].buf); free(ma->agr_map); free(ma->AGR_info); if (ma->ntmp_arr) free(ma->tmp_arr); if (ma->nfmt_map) free(ma->fmt_map); // ma->inf freed in bcf_destroy1 free(ma->d); free(ma->nbuf); for (i=0; imals; i++) free(ma->als[i]); if (ma->mout_als) free(ma->out_als); free(ma->als); free(ma->cnt); free(ma->smpl_ploidy); free(ma->smpl_nGsize); free(ma->has_line); free(ma); } void maux_expand1(maux_t *ma, int i) { if ( ma->nbuf[i] <= ma->files->readers[i].nbuffer ) { int n = ma->files->readers[i].nbuffer + 1; ma->d[i] = (maux1_t*) realloc(ma->d[i], sizeof(maux1_t)*n); memset(ma->d[i]+ma->nbuf[i],0,sizeof(maux1_t)*(n-ma->nbuf[i])); ma->nbuf[i] = n; } } void maux_reset(maux_t *ma) { int i; for (i=0; in; i++) maux_expand1(ma, i); for (i=1; incnt; i++) ma->cnt[i] = 0; } void maux_debug(maux_t *ma, int ir, int ib) { printf("[%d,%d]\t", ir,ib); int i; for (i=0; inals; i++) { printf(" %s [%d]", ma->als[i], ma->cnt[i]); } printf("\n"); } void merge_chrom2qual(args_t *args, bcf1_t *out) { bcf_srs_t *files = args->files; bcf_hdr_t *out_hdr = args->out_hdr; int i, ret; khiter_t kitr; strdict_t *tmph = args->tmph; kh_clear(strdict, tmph); kstring_t *tmps = &args->tmps; tmps->l = 0; maux_t *ma = args->maux; int *al_idxs = (int*) calloc(ma->nals,sizeof(int)); bcf_float_set_missing(out->qual); // CHROM, POS, ID, QUAL out->pos = -1; for (i=0; inreaders; i++) { if ( !ma->has_line[i] ) continue; bcf_sr_t *reader = &files->readers[i]; bcf1_t *line = reader->buffer[0]; bcf_hdr_t *hdr = reader->header; // alleles int j; for (j=1; jn_allele; j++) al_idxs[ ma->d[i][0].map[j] ] = 1; // position if ( out->pos==-1 ) { const char *chr = hdr->id[BCF_DT_CTG][line->rid].key; out->rid = bcf_hdr_name2id(out_hdr, chr); if ( strcmp(chr,out_hdr->id[BCF_DT_CTG][out->rid].key) ) error("Uh\n"); out->pos = line->pos; } // ID if ( line->d.id[0]!='.' || line->d.id[1] ) { kitr = kh_get(strdict, tmph, line->d.id); if ( kitr == kh_end(tmph) ) { if ( tmps->l ) kputc(';', tmps); kputs(line->d.id, tmps); kh_put(strdict, tmph, line->d.id, &ret); } } // set QUAL to the max qual value. Not exactly correct, but good enough for now if ( !bcf_float_is_missing(files->readers[i].buffer[0]->qual) ) { if ( bcf_float_is_missing(out->qual) || out->qual < files->readers[i].buffer[0]->qual ) out->qual = files->readers[i].buffer[0]->qual; } } // set ID if ( !tmps->l ) kputs(".", tmps); if ( out->d.id ) free(out->d.id); out->d.id = strdup(tmps->s); // set alleles ma->nout_als = 0; for (i=1; inals; i++) { if ( !al_idxs[i] ) continue; ma->nout_als++; // Adjust the indexes, the allele map could be created for multiple collapsed records, // some of which might be unused for this output line int ir, j; for (ir=0; irnreaders; ir++) { if ( !ma->has_line[ir] ) continue; bcf1_t *line = files->readers[ir].buffer[0]; for (j=1; jn_allele; j++) if ( ma->d[ir][0].map[j]==i ) ma->d[ir][0].map[j] = ma->nout_als; } } // Expand the arrays and realloc the alleles string. Note that all alleles are in a single allocated block. ma->nout_als++; hts_expand0(char*, ma->nout_als, ma->mout_als, ma->out_als); int k = 0; for (i=0; inals; i++) if ( i==0 || al_idxs[i] ) ma->out_als[k++] = strdup(ma->als[i]); assert( k==ma->nout_als ); normalize_alleles(ma->out_als, ma->nout_als); bcf_update_alleles(out_hdr, out, (const char**) ma->out_als, ma->nout_als); free(al_idxs); for (i=0; inout_als; i++) free(ma->out_als[i]); } void merge_filter(args_t *args, bcf1_t *out) { bcf_srs_t *files = args->files; bcf_hdr_t *out_hdr = args->out_hdr; int i, ret; khiter_t kitr; strdict_t *tmph = args->tmph; kh_clear(strdict, tmph); maux_t *ma = args->maux; out->d.n_flt = 0; for (i=0; inreaders; i++) { if ( !ma->has_line[i]) continue; bcf_sr_t *reader = &files->readers[i]; bcf1_t *line = reader->buffer[0]; bcf_hdr_t *hdr = reader->header; bcf_unpack(line, BCF_UN_ALL); int k; for (k=0; kd.n_flt; k++) { const char *flt = hdr->id[BCF_DT_ID][line->d.flt[k]].key; kitr = kh_get(strdict, tmph, flt); if ( kitr == kh_end(tmph) ) { int id = bcf_hdr_id2int(out_hdr, BCF_DT_ID, flt); if ( id==-1 ) error("The filter not defined: %s\n", flt); hts_expand(int,out->d.n_flt+1,ma->mflt,ma->flt); ma->flt[out->d.n_flt] = id; out->d.n_flt++; kh_put(strdict, tmph, flt, &ret); } } } // Check if PASS is not mixed with other filters if ( out->d.n_flt>1 ) { int id = bcf_hdr_id2int(out_hdr, BCF_DT_ID, "PASS"); for (i=0; id.n_flt; i++) if ( ma->flt[i]==id ) break; if ( id.n_flt ) { out->d.n_flt--; for (; id.n_flt; i++) ma->flt[i] = ma->flt[i+1]; } } out->d.flt = ma->flt; } static void bcf_info_set_id(bcf1_t *line, bcf_info_t *info, int id, kstring_t *tmp_str) { assert( !info->vptr_free ); uint8_t *ptr = info->vptr - info->vptr_off; bcf_dec_typed_int1(ptr, &ptr); tmp_str->l = 0; bcf_enc_int1(tmp_str, id); if ( tmp_str->l == ptr - info->vptr + info->vptr_off ) { // the new id is represented with the same number of bytes memcpy(info->vptr - info->vptr_off, tmp_str->s, tmp_str->l); return; } kputsn_(ptr, info->vptr - ptr, tmp_str); info->vptr_off = tmp_str->l; kputsn_(info->vptr, info->len << bcf_type_shift[info->type], tmp_str); info->vptr = (uint8_t*) tmp_str->s + info->vptr_off; info->vptr_free = 1; line->d.shared_dirty |= BCF1_DIRTY_INF; tmp_str->s = NULL; tmp_str->m = 0; tmp_str->l = 0; } /* * copy_string_field() - copy a comma-separated field * @param src: source string * @param isrc: index of the field to copy * @param src_len: length of source string (excluding the terminating \0) * @param dst: destination kstring (must be initialized) * @param idst: index of the destination field */ int copy_string_field(char *src, int isrc, int src_len, kstring_t *dst, int idst) { int ith_src = 0, start_src = 0; // i-th field in src string while ( ith_srcl ) { if ( dst->s[start_dst]==',' ) { ith_dst++; } start_dst++; } if ( ith_dst!=idst ) return -2; int end_dst = start_dst; while ( end_dstl && dst->s[end_dst]!=',' ) end_dst++; if ( end_dst - start_dst>1 || dst->s[start_dst]!='.' ) return 0; // do not overwrite non-empty values // Now start_dst and end_dst are indexes to the destination memory area // which needs to be replaced with nsrc_cpy // source bytes, end_dst points just after. int ndst_shift = nsrc_cpy - (end_dst - start_dst); int ndst_move = dst->l - end_dst + 1; // how many bytes must be moved (including \0) if ( ndst_shift ) { ks_resize(dst, dst->l + ndst_shift + 1); // plus \0 memmove(dst->s+end_dst+ndst_shift, dst->s+end_dst, ndst_move); } memcpy(dst->s+start_dst, src+start_src, nsrc_cpy); dst->l += ndst_shift; return 0; } static void merge_AGR_info_tag(bcf_hdr_t *hdr, bcf1_t *line, bcf_info_t *info, int len, maux1_t *als, AGR_info_t *agr) { int i; if ( !agr->nbuf ) { if ( info->type==BCF_BT_INT8 || info->type==BCF_BT_INT16 || info->type==BCF_BT_INT32 || info->type==BCF_BT_FLOAT ) { agr->nbuf = 4 * agr->nvals; hts_expand(uint8_t,agr->nbuf,agr->mbuf,agr->buf); if ( info->type!=BCF_BT_FLOAT ) { int32_t *tmp = (int32_t*) agr->buf; for (i=0; invals; i++) tmp[i] = bcf_int32_missing; } else { float *tmp = (float*) agr->buf; for (i=0; invals; i++) bcf_float_set_missing(tmp[i]); } } else if ( info->type==BCF_BT_CHAR ) { kstring_t tmp; tmp.l = 0; tmp.m = agr->mbuf; tmp.s = (char*)agr->buf; kputc('.',&tmp); for (i=1; invals; i++) kputs(",.",&tmp); agr->mbuf = tmp.m; agr->nbuf = tmp.l; agr->buf = (uint8_t*)tmp.s; } else error("Not ready for type [%d]: %s at %d\n", info->type,agr->hdr_tag,line->pos+1); } if ( info->type==BCF_BT_INT8 || info->type==BCF_BT_INT16 || info->type==BCF_BT_INT32 || info->type==BCF_BT_FLOAT ) { if ( len==BCF_VL_A || len==BCF_VL_R ) { int ifrom = len==BCF_VL_A ? 1 : 0; #define BRANCH(type_t, is_missing, is_vector_end, out_type_t) { \ type_t *src = (type_t *) info->vptr; \ out_type_t *tgt = (out_type_t *) agr->buf; \ int iori, inew; \ for (iori=ifrom; iorin_allele; iori++) \ { \ if ( is_vector_end ) break; \ if ( is_missing ) continue; \ inew = als->map[iori] - ifrom; \ tgt[inew] = *src; \ src++; \ } \ } switch (info->type) { case BCF_BT_INT8: BRANCH(int8_t, *src==bcf_int8_missing, *src==bcf_int8_vector_end, int); break; case BCF_BT_INT16: BRANCH(int16_t, *src==bcf_int16_missing, *src==bcf_int16_vector_end, int); break; case BCF_BT_INT32: BRANCH(int32_t, *src==bcf_int32_missing, *src==bcf_int32_vector_end, int); break; case BCF_BT_FLOAT: BRANCH(float, bcf_float_is_missing(*src), bcf_float_is_vector_end(*src), float); break; default: fprintf(stderr,"TODO: %s:%d .. info->type=%d\n", __FILE__,__LINE__, info->type); exit(1); } #undef BRANCH } else { #define BRANCH(type_t, is_missing, is_vector_end, out_type_t) { \ type_t *src = (type_t *) info->vptr; \ out_type_t *tgt = (out_type_t *) agr->buf; \ int iori,jori, inew,jnew; \ for (iori=0; iorin_allele; iori++) \ { \ inew = als->map[iori]; \ for (jori=0; jori<=iori; jori++) \ { \ jnew = als->map[jori]; \ int kori = iori*(iori+1)/2 + jori; \ if ( is_vector_end ) break; \ if ( is_missing ) continue; \ int knew = inew>jnew ? inew*(inew+1)/2 + jnew : jnew*(jnew+1)/2 + inew; \ tgt[knew] = src[kori]; \ } \ if ( jori<=iori ) break; \ } \ } switch (info->type) { case BCF_BT_INT8: BRANCH(int8_t, src[kori]==bcf_int8_missing, src[kori]==bcf_int8_vector_end, int); break; case BCF_BT_INT16: BRANCH(int16_t, src[kori]==bcf_int16_missing, src[kori]==bcf_int16_vector_end, int); break; case BCF_BT_INT32: BRANCH(int32_t, src[kori]==bcf_int32_missing, src[kori]==bcf_int32_vector_end, int); break; case BCF_BT_FLOAT: BRANCH(float, bcf_float_is_missing(src[kori]), bcf_float_is_vector_end(src[kori]), float); break; default: fprintf(stderr,"TODO: %s:%d .. info->type=%d\n", __FILE__,__LINE__, info->type); exit(1); } #undef BRANCH } } else { kstring_t tmp; tmp.l = agr->nbuf; tmp.m = agr->mbuf; tmp.s = (char*)agr->buf; if ( len==BCF_VL_A || len==BCF_VL_R ) { int iori, ifrom = len==BCF_VL_A ? 1 : 0; for (iori=ifrom; iorin_allele; iori++) { int ret = copy_string_field((char*)info->vptr, iori-ifrom, info->len, &tmp, als->map[iori]-ifrom); if ( ret ) error("Error at %s:%d: wrong number of fields in %s?\n", bcf_seqname(hdr,line),line->pos+1,agr->hdr_tag); } } else { int iori,jori, inew,jnew; for (iori=0; iorin_allele; iori++) { inew = als->map[iori]; for (jori=0; jori<=iori; jori++) { jnew = als->map[jori]; int kori = iori*(iori+1)/2 + jori; int knew = bcf_alleles2gt(inew,jnew); int ret = copy_string_field((char*)info->vptr, kori, info->len, &tmp, knew); if ( ret ) error("Error at %s:%d: wrong number of fields in %s?\n", bcf_seqname(hdr,line),line->pos+1,agr->hdr_tag); } } } agr->mbuf = tmp.m; agr->nbuf = tmp.l; agr->buf = (uint8_t*)tmp.s; } } void merge_info(args_t *args, bcf1_t *out) { bcf_srs_t *files = args->files; bcf_hdr_t *out_hdr = args->out_hdr; int i, j, ret; khiter_t kitr; strdict_t *tmph = args->tmph; kh_clear(strdict, tmph); maux_t *ma = args->maux; ma->nAGR_info = 0; out->n_info = 0; info_rules_reset(args); for (i=0; inreaders; i++) { if ( !ma->has_line[i] ) continue; bcf_sr_t *reader = &files->readers[i]; bcf1_t *line = reader->buffer[0]; bcf_hdr_t *hdr = reader->header; for (j=0; jn_info; j++) { bcf_info_t *inf = &line->d.info[j]; const char *key = hdr->id[BCF_DT_ID][inf->key].key; if ( !strcmp("AC",key) || !strcmp("AN",key) ) continue; // AC and AN are done in merge_format() after genotypes are done int id = bcf_hdr_id2int(out_hdr, BCF_DT_ID, key); if ( id==-1 ) error("Error: The INFO field is not defined in the header: %s\n", key); kitr = kh_get(strdict, tmph, key); // have we seen the tag in one of the readers? int len = bcf_hdr_id2length(hdr,BCF_HL_INFO,inf->key); if ( args->nrules ) { info_rule_t *rule = (info_rule_t*) bsearch(key, args->rules, args->nrules, sizeof(*args->rules), info_rules_comp_key); if ( rule ) { maux1_t *als = ( len==BCF_VL_A || len==BCF_VL_G || len==BCF_VL_R ) ? &ma->d[i][0] : NULL; if ( info_rules_add_values(args, hdr, line, rule, als, len) ) continue; } } // Todo: Number=AGR tags should use the newer info_rules_* functions (info_rules_merge_first to be added) // and merge_AGR_info_tag to be made obsolete. if ( len==BCF_VL_A || len==BCF_VL_G || len==BCF_VL_R ) // Number=R,G,A requires special treatment { if ( kitr == kh_end(tmph) ) { // first occurance in this reader, alloc arrays ma->nAGR_info++; hts_expand0(AGR_info_t,ma->nAGR_info,ma->mAGR_info,ma->AGR_info); kitr = kh_put(strdict, tmph, key, &ret); kh_val(tmph,kitr) = ma->nAGR_info - 1; ma->AGR_info[ma->nAGR_info-1].hdr_tag = key; ma->AGR_info[ma->nAGR_info-1].type = bcf_hdr_id2type(hdr,BCF_HL_INFO,inf->key); ma->AGR_info[ma->nAGR_info-1].nbuf = 0; // size of the buffer switch (len) { case BCF_VL_A: ma->AGR_info[ma->nAGR_info-1].nvals = ma->nout_als - 1; break; case BCF_VL_G: ma->AGR_info[ma->nAGR_info-1].nvals = bcf_alleles2gt(ma->nout_als-1,ma->nout_als-1)+1; break; case BCF_VL_R: ma->AGR_info[ma->nAGR_info-1].nvals = ma->nout_als; break; } } kitr = kh_get(strdict, tmph, key); int idx = kh_val(tmph, kitr); if ( idx<0 ) error("Error occurred while processing INFO tag \"%s\" at %s:%d\n", key,bcf_seqname(hdr,line),line->pos+1); merge_AGR_info_tag(hdr, line,inf,len,&ma->d[i][0],&ma->AGR_info[idx]); continue; } if ( kitr == kh_end(tmph) ) { hts_expand0(bcf_info_t,out->n_info+1,ma->minf,ma->inf); ma->inf[out->n_info].key = id; ma->inf[out->n_info].type = inf->type; ma->inf[out->n_info].len = inf->len; ma->inf[out->n_info].vptr = inf->vptr; ma->inf[out->n_info].v1.i = inf->v1.i; ma->inf[out->n_info].v1.f = inf->v1.f; ma->inf[out->n_info].vptr_off = inf->vptr_off; ma->inf[out->n_info].vptr_len = inf->vptr_len; ma->inf[out->n_info].vptr_free = inf->vptr_free; if ( (args->output_type & FT_BCF) && id!=bcf_hdr_id2int(hdr, BCF_DT_ID, key) ) { // The existing packed info cannot be reused. Change the id. // Although quite hacky, it's faster than anything else given // the data structures bcf_info_set_id(out, &ma->inf[out->n_info], id, &args->tmps); } out->n_info++; kitr = kh_put(strdict, tmph, key, &ret); kh_val(tmph,kitr) = -(out->n_info-1); // arbitrary negative value } } } out->d.info = ma->inf; out->d.m_info = ma->minf; for (i=0; inrules; i++) args->rules[i].merger(args->out_hdr, out, &args->rules[i]); for (i=0; inAGR_info; i++) { AGR_info_t *agr = &ma->AGR_info[i]; bcf_update_info(out_hdr,out,agr->hdr_tag,agr->buf,agr->nvals,agr->type); } } void update_AN_AC(bcf_hdr_t *hdr, bcf1_t *line) { int32_t an = 0, *tmp = (int32_t*) malloc(sizeof(int)*line->n_allele); int ret = bcf_calc_ac(hdr, line, tmp, BCF_UN_FMT); if ( ret>0 ) { int i; for (i=0; in_allele; i++) an += tmp[i]; bcf_update_info_int32(hdr, line, "AN", &an, 1); bcf_update_info_int32(hdr, line, "AC", tmp+1, line->n_allele-1); } free(tmp); } void merge_GT(args_t *args, bcf_fmt_t **fmt_map, bcf1_t *out) { bcf_srs_t *files = args->files; bcf_hdr_t *out_hdr = args->out_hdr; maux_t *ma = args->maux; int i, ismpl = 0, nsamples = bcf_hdr_nsamples(out_hdr); int nsize = 0, msize = sizeof(int32_t); for (i=0; inreaders; i++) { if ( !fmt_map[i] ) continue; if ( fmt_map[i]->n > nsize ) nsize = fmt_map[i]->n; } if ( ma->ntmp_arr < nsamples*nsize*msize ) { ma->ntmp_arr = nsamples*nsize*msize; ma->tmp_arr = realloc(ma->tmp_arr, ma->ntmp_arr); } memset(ma->smpl_ploidy,0,nsamples*sizeof(int)); for (i=0; inreaders; i++) { bcf_sr_t *reader = &files->readers[i]; bcf_hdr_t *hdr = reader->header; bcf_fmt_t *fmt_ori = fmt_map[i]; int32_t *tmp = (int32_t *) ma->tmp_arr + ismpl*nsize; int j, k; if ( !fmt_ori ) { // missing values: assume maximum ploidy for (j=0; jsmpl_ploidy[ismpl+j]++; } tmp += nsize; } ismpl += bcf_hdr_nsamples(hdr); continue; } #define BRANCH(type_t, vector_end) { \ type_t *p_ori = (type_t*) fmt_ori->p; \ if ( !ma->d[i][0].als_differ ) \ { \ /* the allele numbering is unchanged */ \ for (j=0; jn; k++) \ { \ if ( p_ori[k]==vector_end ) break; /* smaller ploidy */ \ ma->smpl_ploidy[ismpl+j]++; \ if ( bcf_gt_is_missing(p_ori[k]) ) tmp[k] = 0; /* missing allele */ \ else tmp[k] = p_ori[k]; \ } \ for (; kn; \ } \ ismpl += bcf_hdr_nsamples(hdr); \ continue; \ } \ /* allele numbering needs to be changed */ \ for (j=0; jn; k++) \ { \ if ( p_ori[k]==vector_end ) break; /* smaller ploidy */ \ ma->smpl_ploidy[ismpl+j]++; \ if ( bcf_gt_is_missing(p_ori[k]) ) tmp[k] = 0; /* missing allele */ \ else \ { \ int al = (p_ori[k]>>1) - 1; \ al = al<=0 ? al + 1 : ma->d[i][0].map[al] + 1; \ tmp[k] = (al << 1) | ((p_ori[k])&1); \ } \ } \ for (; kn; \ } \ ismpl += bcf_hdr_nsamples(hdr); \ } switch (fmt_ori->type) { case BCF_BT_INT8: BRANCH(int8_t, bcf_int8_vector_end); break; case BCF_BT_INT16: BRANCH(int16_t, bcf_int16_vector_end); break; case BCF_BT_INT32: BRANCH(int32_t, bcf_int32_vector_end); break; default: error("Unexpected case: %d\n", fmt_ori->type); } #undef BRANCH } bcf_update_format_int32(out_hdr, out, "GT", (int32_t*)ma->tmp_arr, nsamples*nsize); } void merge_format_field(args_t *args, bcf_fmt_t **fmt_map, bcf1_t *out) { bcf_srs_t *files = args->files; bcf_hdr_t *out_hdr = args->out_hdr; maux_t *ma = args->maux; int i, ismpl = 0, nsamples = bcf_hdr_nsamples(out_hdr); const char *key = NULL; int nsize = 0, length = BCF_VL_FIXED, type = -1; for (i=0; inreaders; i++) { if ( !ma->has_line[i] ) continue; if ( !fmt_map[i] ) continue; if ( !key ) key = files->readers[i].header->id[BCF_DT_ID][fmt_map[i]->id].key; type = fmt_map[i]->type; if ( IS_VL_G(files->readers[i].header, fmt_map[i]->id) ) { length = BCF_VL_G; nsize = out->n_allele*(out->n_allele + 1)/2; break; } if ( IS_VL_A(files->readers[i].header, fmt_map[i]->id) ) { length = BCF_VL_A; nsize = out->n_allele - 1; break; } if ( IS_VL_R(files->readers[i].header, fmt_map[i]->id) ) { length = BCF_VL_R; nsize = out->n_allele; break; } if ( fmt_map[i]->n > nsize ) nsize = fmt_map[i]->n; } int msize = sizeof(float)>sizeof(int32_t) ? sizeof(float) : sizeof(int32_t); if ( ma->ntmp_arr < nsamples*nsize*msize ) { ma->ntmp_arr = nsamples*nsize*msize; ma->tmp_arr = realloc(ma->tmp_arr, ma->ntmp_arr); } // Fill the temp array for all samples by collecting values from all files for (i=0; inreaders; i++) { bcf_sr_t *reader = &files->readers[i]; bcf_hdr_t *hdr = reader->header; bcf_fmt_t *fmt_ori = fmt_map[i]; if ( fmt_ori ) { type = fmt_ori->type; int nals_ori = reader->buffer[0]->n_allele; if ( length==BCF_VL_G ) { // if all fields are missing then n==1 is valid if ( fmt_ori->n!=1 && fmt_ori->n != nals_ori*(nals_ori+1)/2 && fmt_map[i]->n != nals_ori ) error("Incorrect number of %s fields (%d) at %s:%d, cannot merge.\n", key,fmt_ori->n,bcf_seqname(args->out_hdr,out),out->pos+1); } else if ( length==BCF_VL_A ) { if ( fmt_ori->n!=1 && fmt_ori->n != nals_ori-1 ) error("Incorrect number of %s fields (%d) at %s:%d, cannot merge.\n", key,fmt_ori->n,bcf_seqname(args->out_hdr,out),out->pos+1); } else if ( length==BCF_VL_R ) { if ( fmt_ori->n!=1 && fmt_ori->n != nals_ori ) error("Incorrect number of %s fields (%d) at %s:%d, cannot merge.\n", key,fmt_ori->n,bcf_seqname(args->out_hdr,out),out->pos+1); } } // set the values #define BRANCH(tgt_type_t, src_type_t, src_is_missing, src_is_vector_end, tgt_set_missing, tgt_set_vector_end) { \ int j, l, k; \ tgt_type_t *tgt = (tgt_type_t *) ma->tmp_arr + ismpl*nsize; \ if ( !fmt_ori ) \ { \ /* the field is not present in this file, set missing values */ \ for (j=0; jhas_line[i] ); \ bcf1_t *line = reader->buffer[0]; \ src_type_t *src = (src_type_t*) fmt_ori->p; \ if ( (length!=BCF_VL_G && length!=BCF_VL_A && length!=BCF_VL_R) || (line->n_allele==out->n_allele && !ma->d[i][0].als_differ) ) \ { \ /* alleles unchanged, copy over */ \ for (j=0; jn; l++) \ { \ if ( src_is_vector_end ) break; \ else if ( src_is_missing ) tgt_set_missing; \ else *tgt = *src; \ tgt++; src++; \ } \ for (k=l; kn - l; \ } \ ismpl += bcf_hdr_nsamples(hdr); \ continue; \ } \ /* allele numbering needs to be changed */ \ if ( length==BCF_VL_G ) \ { \ /* Number=G tags */ \ for (j=0; jtmp_arr + (ismpl+j)*nsize; \ src = (src_type_t*) fmt_ori->p + j*fmt_ori->n; \ if ( (src_is_missing && fmt_ori->n==1) || (++src && src_is_vector_end) ) \ { \ /* tag with missing value "." */ \ tgt_set_missing; \ for (l=1; lsmpl_ploidy[ismpl+j]==1 ? out->n_allele : out->n_allele*(out->n_allele + 1)/2; \ for (l=0; lsmpl_ploidy[ismpl+j]==1 ) \ { \ /* Haploid */ \ int iori, inew; \ for (iori=0; iorin_allele; iori++) \ { \ inew = ma->d[i][0].map[iori]; \ src = (src_type_t*) fmt_ori->p + j*fmt_ori->n + iori; \ tgt = (tgt_type_t *) ma->tmp_arr + (ismpl+j)*nsize + inew; \ if ( src_is_vector_end ) break; \ if ( src_is_missing ) tgt_set_missing; \ else *tgt = *src; \ } \ } \ else \ { \ /* Diploid */ \ int iori,jori, inew,jnew; \ for (iori=0; iorin_allele; iori++) \ { \ inew = ma->d[i][0].map[iori]; \ for (jori=0; jori<=iori; jori++) \ { \ jnew = ma->d[i][0].map[jori]; \ int kori = iori*(iori+1)/2 + jori; \ int knew = inew>jnew ? inew*(inew+1)/2 + jnew : jnew*(jnew+1)/2 + inew; \ src = (src_type_t*) fmt_ori->p + j*fmt_ori->n + kori; \ tgt = (tgt_type_t *) ma->tmp_arr + (ismpl+j)*nsize + knew; \ if ( src_is_vector_end ) \ { \ iori = line->n_allele; \ break; \ } \ if ( src_is_missing ) tgt_set_missing; \ else *tgt = *src; \ } \ } \ } \ } \ } \ else \ { \ /* Number=A or Number=R tags */ \ int ifrom = length==BCF_VL_A ? 1 : 0; \ for (j=0; jtmp_arr + (ismpl+j)*nsize; \ src = (src_type_t*) (fmt_ori->p + j*fmt_ori->size); \ if ( (src_is_missing && fmt_ori->n==1) || (++src && src_is_vector_end) ) \ { \ /* tag with missing value "." */ \ tgt_set_missing; \ for (l=1; lp + j*fmt_ori->size); \ for (l=0; ln_allele; iori++) \ { \ inew = ma->d[i][0].map[iori] - ifrom; \ tgt = (tgt_type_t *) ma->tmp_arr + (ismpl+j)*nsize + inew; \ if ( src_is_vector_end ) break; \ if ( src_is_missing ) tgt_set_missing; \ else *tgt = *src; \ src++; \ } \ } \ } \ ismpl += bcf_hdr_nsamples(hdr); \ } switch (type) { case BCF_BT_INT8: BRANCH(int32_t, int8_t, *src==bcf_int8_missing, *src==bcf_int8_vector_end, *tgt=bcf_int32_missing, *tgt=bcf_int32_vector_end); break; case BCF_BT_INT16: BRANCH(int32_t, int16_t, *src==bcf_int16_missing, *src==bcf_int16_vector_end, *tgt=bcf_int32_missing, *tgt=bcf_int32_vector_end); break; case BCF_BT_INT32: BRANCH(int32_t, int32_t, *src==bcf_int32_missing, *src==bcf_int32_vector_end, *tgt=bcf_int32_missing, *tgt=bcf_int32_vector_end); break; case BCF_BT_FLOAT: BRANCH(float, float, bcf_float_is_missing(*src), bcf_float_is_vector_end(*src), bcf_float_set_missing(*tgt), bcf_float_set_vector_end(*tgt)); break; case BCF_BT_CHAR: BRANCH(uint8_t, uint8_t, *src==bcf_str_missing, *src==bcf_str_vector_end, *tgt=bcf_str_missing, *tgt=bcf_str_vector_end); break; default: error("Unexpected case: %d, %s\n", type, key); } #undef BRANCH } if ( type==BCF_BT_FLOAT ) bcf_update_format_float(out_hdr, out, key, (float*)ma->tmp_arr, nsamples*nsize); else if ( type==BCF_BT_CHAR ) bcf_update_format_char(out_hdr, out, key, (float*)ma->tmp_arr, nsamples*nsize); else bcf_update_format_int32(out_hdr, out, key, (int32_t*)ma->tmp_arr, nsamples*nsize); } void merge_format(args_t *args, bcf1_t *out) { bcf_srs_t *files = args->files; bcf_hdr_t *out_hdr = args->out_hdr; maux_t *ma = args->maux; if ( !ma->nfmt_map ) { ma->nfmt_map = 2; ma->fmt_map = (bcf_fmt_t**) calloc(ma->nfmt_map*files->nreaders, sizeof(bcf_fmt_t*)); } else memset(ma->fmt_map, 0, ma->nfmt_map*files->nreaders*sizeof(bcf_fmt_t**)); khiter_t kitr; strdict_t *tmph = args->tmph; kh_clear(strdict, tmph); int i, j, ret, has_GT = 0, max_ifmt = 0; // max fmt index for (i=0; inreaders; i++) { if ( !ma->has_line[i] ) continue; bcf_sr_t *reader = &files->readers[i]; bcf1_t *line = reader->buffer[0]; bcf_hdr_t *hdr = reader->header; for (j=0; jn_fmt; j++) { // Wat this tag already seen? bcf_fmt_t *fmt = &line->d.fmt[j]; const char *key = hdr->id[BCF_DT_ID][fmt->id].key; kitr = kh_get(strdict, tmph, key); int ifmt; if ( kitr != kh_end(tmph) ) ifmt = kh_value(tmph, kitr); // seen else { // new FORMAT tag if ( key[0]=='G' && key[1]=='T' && key[2]==0 ) { has_GT = 1; ifmt = 0; } else { ifmt = ++max_ifmt; if ( max_ifmt >= ma->nfmt_map ) { ma->fmt_map = (bcf_fmt_t**) realloc(ma->fmt_map, sizeof(bcf_fmt_t*)*(max_ifmt+1)*files->nreaders); memset(ma->fmt_map+ma->nfmt_map*files->nreaders, 0, (max_ifmt-ma->nfmt_map+1)*files->nreaders*sizeof(bcf_fmt_t*)); ma->nfmt_map = max_ifmt+1; } } kitr = kh_put(strdict, tmph, key, &ret); kh_value(tmph, kitr) = ifmt; } ma->fmt_map[ifmt*files->nreaders+i] = fmt; } // Check if the allele numbering must be changed for (j=1; jbuffer[0]->n_allele; j++) if ( ma->d[i][0].map[j]!=j ) break; ma->d[i][0].als_differ = j==reader->buffer[0]->n_allele ? 0 : 1; } out->n_sample = bcf_hdr_nsamples(out_hdr); if ( has_GT ) merge_GT(args, ma->fmt_map, out); update_AN_AC(out_hdr, out); if ( out->d.info!=ma->inf ) { // hacky, we rely on htslib internals: bcf_update_info() reallocated the info ma->inf = out->d.info; ma->minf = out->d.m_info; } for (i=1; i<=max_ifmt; i++) merge_format_field(args, &ma->fmt_map[i*files->nreaders], out); out->d.indiv_dirty = 1; } // The core merging function, one or none line from each reader void merge_line(args_t *args) { bcf1_t *out = args->out_line; bcf_clear1(out); out->unpacked = BCF_UN_ALL; merge_chrom2qual(args, out); merge_filter(args, out); merge_info(args, out); merge_format(args, out); bcf_write1(args->out_fh, args->out_hdr, out); } void debug_buffers(FILE *fp, bcf_srs_t *files); void debug_buffer(FILE *fp, bcf_sr_t *reader); #define SWAP(type_t,a,b) { type_t tmp = (a); (a) = (b); (b) = tmp; } // Clean the reader's buffer to and make it ready for the next next_line() call. // Moves finished records (SKIP_DONE flag set) at the end of the buffer and put // the rest to the beggining. Then shorten the buffer so that the last element // points to the last unfinished record. There are two special cases: the last // line of the buffer typically has a different position and must stay at the // end; next, the first record of the buffer must be one of those already // printed, as it will be discarded by next_line(). // void shake_buffer(maux_t *maux, int ir, int pos) { bcf_sr_t *reader = &maux->files->readers[ir]; maux1_t *m = maux->d[ir]; if ( !reader->buffer ) return; int i; // FILE *fp = stdout; // fprintf(fp," nbuf=%d\t", reader->nbuffer); for (i=0; inbuffer; i++) fprintf(fp," %d", skip[i]); fprintf(fp,"\n"); // debug_buffer(fp,reader); // fprintf(fp,"--\n"); int a = 1, b = reader->nbuffer; if ( reader->buffer[b]->pos != pos ) b--; // move the last line separately afterwards while ( abuffer[a], reader->buffer[b]); SWAP(maux1_t, m[a], m[b]); a++; b--; } // position $a to the after the first unfinished record while ( a<=reader->nbuffer && !(m[a].skip&SKIP_DONE) ) a++; if ( anbuffer ) { // there is a gap between the unfinished lines at the beggining and the // last line. The last line must be brought forward to fill the gap if ( reader->buffer[reader->nbuffer]->pos != pos ) { SWAP(bcf1_t*, reader->buffer[a], reader->buffer[reader->nbuffer]); SWAP(maux1_t, m[a], m[reader->nbuffer]); reader->nbuffer = a; } } if ( !(m[0].skip&SKIP_DONE) && reader->buffer[0]->pos==pos ) { // the first record is unfinished, replace it with an empty line // from the end of the buffer or else next_line will remove it if ( reader->nbuffer + 1 >= maux->nbuf[ir] ) { reader->nbuffer++; maux_expand1(maux, ir); reader->nbuffer--; m = maux->d[ir]; } if ( reader->nbuffer+1 >= reader->mbuffer ) error("Uh, did not expect this: %d vs %d\n", reader->nbuffer,reader->mbuffer); if ( reader->buffer[reader->nbuffer]->pos!=pos ) { // 4way swap bcf1_t *tmp = reader->buffer[0]; reader->buffer[0] = reader->buffer[reader->nbuffer+1]; reader->buffer[reader->nbuffer+1] = reader->buffer[reader->nbuffer]; reader->buffer[reader->nbuffer] = tmp; m[reader->nbuffer].skip = m[0].skip; m[reader->nbuffer+1].skip = SKIP_DIFF; reader->nbuffer++; } else { SWAP(bcf1_t*, reader->buffer[0], reader->buffer[reader->nbuffer+1]); SWAP(maux1_t, m[0], m[reader->nbuffer+1]); } } // debug_buffer(fp,reader); // fprintf(fp,"\t"); for (i=0; inbuffer; i++) fprintf(fp," %d", skip[i]); // fprintf(fp,"\n\n"); // set position of finished buffer[0] line to -1, otherwise swapping may // bring it back after next_line() reader->buffer[0]->pos = -1; // trim the buffer, remove finished lines from the end i = reader->nbuffer; while ( i>=1 && m[i--].skip&SKIP_DONE ) reader->nbuffer--; } void debug_maux(args_t *args, int pos, int var_type) { bcf_srs_t *files = args->files; maux_t *maux = args->maux; int j,k,l; fprintf(stderr,"Alleles to merge at %d\n", pos+1); for (j=0; jnreaders; j++) { bcf_sr_t *reader = &files->readers[j]; fprintf(stderr," reader %d: ", j); for (k=0; k<=reader->nbuffer; k++) { if ( maux->d[j][k].skip==SKIP_DONE ) continue; bcf1_t *line = reader->buffer[k]; if ( line->pos!=pos ) continue; fprintf(stderr,"\t"); if ( maux->d[j][k].skip ) fprintf(stderr,"["); // this record will not be merged in this round for (l=0; ln_allele; l++) fprintf(stderr,"%s%s", l==0?"":",", line->d.allele[l]); if ( maux->d[j][k].skip ) fprintf(stderr,"]"); } fprintf(stderr,"\n"); } fprintf(stderr," counts: "); for (j=0; jnals; j++) fprintf(stderr,"%s %dx %s", j==0?"":",",maux->cnt[j], maux->als[j]); fprintf(stderr,"\n"); for (j=0; jnreaders; j++) { bcf_sr_t *reader = &files->readers[j]; fprintf(stderr," out %d: ", j); for (k=0; k<=reader->nbuffer; k++) { if ( maux->d[j][k].skip==SKIP_DONE ) continue; bcf1_t *line = reader->buffer[k]; if ( line->pos!=pos ) continue; if ( maux->d[j][k].skip ) continue; fprintf(stderr,"\t"); for (l=0; ln_allele; l++) fprintf(stderr,"%s%s", l==0?"":",", maux->als[maux->d[j][k].map[l]]); } fprintf(stderr,"\n"); } fprintf(stderr,"\n"); } // Determine which line should be merged from which reader: go through all // readers and all buffered lines, expand REF,ALT and try to match lines with // the same ALTs. A step towards output independent on input ordering of the // lines. void merge_buffer(args_t *args) { bcf_srs_t *files = args->files; int i, pos = -1, var_type = 0; char *id = NULL; maux_t *maux = args->maux; maux_reset(maux); // set the current position for (i=0; inreaders; i++) { if ( bcf_sr_has_line(files,i) ) { bcf1_t *line = bcf_sr_get_line(files,i); pos = line->pos; var_type = bcf_get_variant_types(line); id = line->d.id; break; } } // In this loop we select from each reader compatible candidate lines. // (i.e. SNPs or indels). Go through all files and all lines at this // position and normalize relevant alleles. // REF-only sites may be associated with both SNPs and indels. for (i=0; inreaders; i++) { bcf_sr_t *reader = &files->readers[i]; if ( !reader->buffer ) continue; int j, k; for (j=0; j<=reader->nbuffer; j++) { bcf1_t *line = reader->buffer[j]; int line_type = bcf_get_variant_types(line); // select relevant lines maux->d[i][j].skip = SKIP_DIFF; if ( pos!=line->pos ) { if ( j==0 ) maux->d[i][j].skip |= SKIP_DONE; // left from previous run, force to ignore continue; } if ( args->merge_by_id ) { if ( strcmp(id,line->d.id) ) continue; } else { if ( args->collapse==COLLAPSE_NONE && maux->nals ) { // All alleles of the tested record must be present in the // selected maux record plus variant types must be the same if ( var_type!=line->d.var_type ) continue; if ( vcmp_set_ref(args->vcmp,maux->als[0],line->d.allele[0]) < 0 ) continue; // refs not compatible for (k=1; kn_allele; k++) { if ( vcmp_find_allele(args->vcmp,maux->als+1,maux->nals-1,line->d.allele[k])>=0 ) break; } if ( k==line->n_allele ) continue; // no matching allele } if ( !(args->collapse&COLLAPSE_ANY) ) { int compatible = 0; if ( line_type==var_type ) compatible = 1; else if ( line_type==VCF_REF ) compatible = 1; // REF can go with anything else if ( var_type&VCF_SNP && line_type&VCF_SNP ) compatible = 1; else if ( var_type&VCF_INDEL && line_type&VCF_INDEL ) compatible = 1; else if ( var_type&VCF_MNP && line_type&VCF_MNP ) compatible = 1; else if ( var_type&VCF_SNP && line_type&VCF_MNP ) compatible = 1; else if ( var_type&VCF_MNP && line_type&VCF_SNP ) compatible = 1; if ( !compatible ) continue; } } maux->d[i][j].skip = 0; hts_expand(int, line->n_allele, maux->d[i][j].mmap, maux->d[i][j].map); if ( !maux->nals ) // first record, copy the alleles to the output { maux->nals = line->n_allele; hts_expand0(char*, maux->nals, maux->mals, maux->als); hts_expand0(int, maux->nals, maux->ncnt, maux->cnt); for (k=0; knals; k++) { maux->als[k] = strdup(line->d.allele[k]); maux->d[i][j].map[k] = k; maux->cnt[k] = 1; } pos = line->pos; continue; } // normalize alleles maux->als = merge_alleles(line->d.allele, line->n_allele, maux->d[i][j].map, maux->als, &maux->nals, &maux->mals); if ( !maux->als ) error("Failed to merge alleles at %s:%d in %s\n",bcf_seqname(args->out_hdr,line),line->pos+1,reader->fname); hts_expand0(int, maux->nals, maux->ncnt, maux->cnt); for (k=1; kn_allele; k++) maux->cnt[ maux->d[i][j].map[k] ]++; // how many times an allele appears in the files maux->cnt[0]++; } } // debug_maux(args, pos, var_type); // Select records that have the same alleles; the input ordering of indels // must not matter. Multiple VCF lines can be emitted from this loop. // We expect only very few alleles and not many records with the same // position in the buffers, therefore the nested loops should not slow us // much. while (1) { // take the most frequent allele present in multiple files int icnt = 0; for (i=1; inals; i++) if ( maux->cnt[i] > maux->cnt[icnt] ) icnt = i; if ( maux->cnt[icnt]<0 ) break; int nmask = 0; for (i=0; inreaders; i++) { maux->has_line[i] = 0; bcf_sr_t *reader = &files->readers[i]; if ( !reader->buffer ) continue; // find lines with the same allele int j; for (j=0; j<=reader->nbuffer; j++) { if ( maux->d[i][j].skip ) continue; int k; for (k=0; kbuffer[j]->n_allele; k++) if ( icnt==maux->d[i][j].map[k] ) break; if ( kbuffer[j]->n_allele ) break; } if ( j>reader->nbuffer ) { // no matching allele found in this file if ( args->collapse==COLLAPSE_NONE ) continue; for (j=0; j<=reader->nbuffer; j++) { if ( maux->d[i][j].skip ) continue; if ( args->collapse&COLLAPSE_ANY ) break; int line_type = bcf_get_variant_types(reader->buffer[j]); if ( var_type&VCF_SNP && line_type&VCF_SNP && (args->collapse&COLLAPSE_SNPS) ) break; if ( var_type&VCF_INDEL && line_type&VCF_INDEL && (args->collapse&COLLAPSE_INDELS) ) break; if ( line_type==VCF_REF ) { if ( var_type&VCF_SNP && (args->collapse&COLLAPSE_SNPS) ) break; if ( var_type&VCF_INDEL && (args->collapse&COLLAPSE_INDELS) ) break; } else if ( var_type==VCF_REF ) { if ( line_type&VCF_SNP && (args->collapse&COLLAPSE_SNPS) ) break; if ( line_type&VCF_INDEL && (args->collapse&COLLAPSE_INDELS) ) break; } } } if ( j<=reader->nbuffer ) { // found a suitable line for merging, place it at the beggining if ( j>0 ) { SWAP(bcf1_t*, reader->buffer[0], reader->buffer[j]); SWAP(maux1_t, maux->d[i][0], maux->d[i][j]); } // mark as finished so that it's ignored next time maux->d[i][0].skip |= SKIP_DONE; maux->has_line[i] = 1; nmask++; } } if ( !nmask ) break; // done, no more lines suitable for merging found merge_line(args); // merge and output the line maux->cnt[icnt] = -1; // do not pick this allele again, mark it as finished } // clean the alleles for (i=0; inals; i++) { free(maux->als[i]); maux->als[i] = 0; } maux->nals = 0; // get the buffers ready for the next next_line() call for (i=0; inreaders; i++) shake_buffer(maux, i, pos); } void bcf_hdr_append_version(bcf_hdr_t *hdr, int argc, char **argv, const char *cmd) { kstring_t str = {0,0,0}; ksprintf(&str,"##%sVersion=%s+htslib-%s\n", cmd, bcftools_version(), hts_version()); bcf_hdr_append(hdr,str.s); str.l = 0; ksprintf(&str,"##%sCommand=%s", cmd, argv[0]); int i; for (i=1; iout_fh = hts_open(args->output_fname, hts_bcf_wmode(args->output_type)); if ( args->out_fh == NULL ) error("Can't write to \"%s\": %s\n", args->output_fname, strerror(errno)); args->out_hdr = bcf_hdr_init("w"); if ( args->header_fname ) { if ( bcf_hdr_set(args->out_hdr,args->header_fname) ) error("Could not read/parse the header: %s\n", args->header_fname); } else { int i; for (i=0; ifiles->nreaders; i++) { char buf[10]; snprintf(buf,10,"%d",i+1); bcf_hdr_merge(args->out_hdr, args->files->readers[i].header,buf,args->force_samples); } bcf_hdr_append_version(args->out_hdr, args->argc, args->argv, "bcftools_merge"); bcf_hdr_sync(args->out_hdr); } info_rules_init(args); bcf_hdr_set_version(args->out_hdr, bcf_hdr_get_version(args->files->readers[0].header)); bcf_hdr_write(args->out_fh, args->out_hdr); if ( args->header_only ) { bcf_hdr_destroy(args->out_hdr); hts_close(args->out_fh); return; } if ( args->collapse==COLLAPSE_NONE ) args->vcmp = vcmp_init(); args->maux = maux_init(args->files); args->out_line = bcf_init1(); args->tmph = kh_init(strdict); int ret; while ( (ret=bcf_sr_next_line(args->files)) ) { merge_buffer(args); } info_rules_destroy(args); maux_destroy(args->maux); bcf_hdr_destroy(args->out_hdr); hts_close(args->out_fh); bcf_destroy1(args->out_line); kh_destroy(strdict, args->tmph); if ( args->tmps.m ) free(args->tmps.s); if ( args->vcmp ) vcmp_destroy(args->vcmp); } static void usage(void) { fprintf(stderr, "\n"); fprintf(stderr, "About: Merge multiple VCF/BCF files from non-overlapping sample sets to create one multi-sample file.\n"); fprintf(stderr, " Note that only records from different files can be merged, never from the same file. For\n"); fprintf(stderr, " \"vertical\" merge take a look at \"bcftools norm\" instead.\n"); fprintf(stderr, "Usage: bcftools merge [options] [...]\n"); fprintf(stderr, "\n"); fprintf(stderr, "Options:\n"); fprintf(stderr, " --force-samples resolve duplicate sample names\n"); fprintf(stderr, " --print-header print only the merged header and exit\n"); fprintf(stderr, " --use-header use the provided header\n"); fprintf(stderr, " -f, --apply-filters require at least one of the listed FILTER strings (e.g. \"PASS,.\")\n"); fprintf(stderr, " -i, --info-rules rules for merging INFO fields (method is one of sum,avg,min,max,join) or \"-\" to turn off the default [DP:sum,DP4:sum]\n"); fprintf(stderr, " -l, --file-list read file names from the file\n"); fprintf(stderr, " -m, --merge allow multiallelic records for , see man page for details [both]\n"); fprintf(stderr, " -o, --output write output to a file [standard output]\n"); fprintf(stderr, " -O, --output-type 'b' compressed BCF; 'u' uncompressed BCF; 'z' compressed VCF; 'v' uncompressed VCF [v]\n"); fprintf(stderr, " -r, --regions restrict to comma-separated list of regions\n"); fprintf(stderr, " -R, --regions-file restrict to regions listed in a file\n"); fprintf(stderr, "\n"); exit(1); } int main_vcfmerge(int argc, char *argv[]) { int c; args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->files = bcf_sr_init(); args->argc = argc; args->argv = argv; args->output_fname = "-"; args->output_type = FT_VCF; args->collapse = COLLAPSE_BOTH; int regions_is_file = 0; static struct option loptions[] = { {"help",0,0,'h'}, {"merge",1,0,'m'}, {"file-list",1,0,'l'}, {"apply-filters",1,0,'f'}, {"use-header",1,0,1}, {"print-header",0,0,2}, {"force-samples",0,0,3}, {"output",1,0,'o'}, {"output-type",1,0,'O'}, {"regions",1,0,'r'}, {"regions-file",1,0,'R'}, {"info-rules",1,0,'i'}, {0,0,0,0} }; while ((c = getopt_long(argc, argv, "hm:f:r:R:o:O:i:l:",loptions,NULL)) >= 0) { switch (c) { case 'l': args->file_list = optarg; break; case 'i': args->info_rules = optarg; break; case 'o': args->output_fname = optarg; break; case 'O': switch (optarg[0]) { case 'b': args->output_type = FT_BCF_GZ; break; case 'u': args->output_type = FT_BCF; break; case 'z': args->output_type = FT_VCF_GZ; break; case 'v': args->output_type = FT_VCF; break; default: error("The output type \"%s\" not recognised\n", optarg); } break; case 'm': args->collapse = COLLAPSE_NONE; if ( !strcmp(optarg,"snps") ) args->collapse |= COLLAPSE_SNPS; else if ( !strcmp(optarg,"indels") ) args->collapse |= COLLAPSE_INDELS; else if ( !strcmp(optarg,"both") ) args->collapse |= COLLAPSE_BOTH; else if ( !strcmp(optarg,"any") ) args->collapse |= COLLAPSE_ANY; else if ( !strcmp(optarg,"all") ) args->collapse |= COLLAPSE_ANY; else if ( !strcmp(optarg,"none") ) args->collapse = COLLAPSE_NONE; else if ( !strcmp(optarg,"id") ) { args->collapse = COLLAPSE_NONE; args->merge_by_id = 1; } else error("The -m type \"%s\" is not recognised.\n", optarg); break; case 'f': args->files->apply_filters = optarg; break; case 'r': args->regions_list = optarg; break; case 'R': args->regions_list = optarg; regions_is_file = 1; break; case 1 : args->header_fname = optarg; break; case 2 : args->header_only = 1; break; case 3 : args->force_samples = 1; break; case 'h': case '?': usage(); default: error("Unknown argument: %s\n", optarg); } } if ( argc==optind && !args->file_list ) usage(); if ( argc-optind<2 && !args->file_list ) usage(); args->files->require_index = 1; if ( args->regions_list && bcf_sr_set_regions(args->files, args->regions_list, regions_is_file)<0 ) error("Failed to read the regions: %s\n", args->regions_list); while (optindfiles, argv[optind]) ) error("Failed to open %s: %s\n", argv[optind],bcf_sr_strerror(args->files->errnum)); optind++; } if ( args->file_list ) { int nfiles, i; char **files = hts_readlines(args->file_list, &nfiles); if ( !files ) error("Failed to read from %s\n", args->file_list); for (i=0;ifiles, files[i]) ) error("Failed to open %s: %s\n", files[i],bcf_sr_strerror(args->files->errnum)); for (i=0; ifiles); free(args); return 0; } bcftools-1.2/vcfnorm.c000066400000000000000000002222611246371514100150160ustar00rootroot00000000000000/* vcfnorm.c -- Left-align and normalize indels. Copyright (C) 2013-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include #include #include "bcftools.h" #include "rbuf.h" #define CHECK_REF_EXIT 0 #define CHECK_REF_WARN 1 #define CHECK_REF_SKIP 2 #define MROWS_SPLIT 1 #define MROWS_MERGE 2 typedef struct { int32_t dir:4, val:28; } cell_t; typedef struct { int nmat, nref, nseq; int ipos, lref, lseq; cell_t *mat; char *ref, *seq; int m_arr, *ipos_arr, *lref_arr, *lseq_arr; } aln_aux_t; // for -m+, mapping from allele indexes of a single input record // to allele indexes of output record typedef struct { int nals, mals, *map; } map_t; typedef struct { aln_aux_t aln; char *tseq, *seq; int mseq; bcf1_t **lines, **tmp_lines, **alines, **blines, *mrow_out; int ntmp_lines, mtmp_lines, nalines, malines, nblines, mblines; map_t *maps; // mrow map for each buffered record char **als; int mmaps, nals, mals; uint8_t *tmp_arr1, *tmp_arr2; int ntmp_arr1, ntmp_arr2; kstring_t *tmp_str; kstring_t *tmp_als, tmp_als_str; int ntmp_als; rbuf_t rbuf; int buf_win; // maximum distance between two records to consider int aln_win; // the realignment window size (maximum repeat size) bcf_srs_t *files; // using the synced reader only for -r option bcf_hdr_t *hdr; faidx_t *fai; char **argv, *output_fname, *ref_fname, *vcf_fname, *region, *targets; int argc, rmdup, output_type, check_ref, strict_filter; int nchanged, nskipped, ntotal, mrows_op, mrows_collapse, parsimonious; } args_t; #if OLD_WAY void _vcfnorm_debug_print(aln_aux_t *aux) { cell_t *mat = aux->mat; char *ref = aux->ref; char *seq = aux->seq; int nref = aux->nref; int nseq = aux->nseq; int k = (nref+1)*(nseq+1)-1; int kd = nref+2; int i = k/(nref+1); int j = k - i*(nref+1); assert(i>0 && j>0); int l = k, ialn = 0, nout_ref = 0, nout_seq = 0, ipos = 0; char *aln_ref = (char*) malloc(sizeof(char)*(k+1)); char *aln_seq = (char*) malloc(sizeof(char)*(k+1)); while ( l>0 ) { if ( j<=0 || mat[l].dir==1 ) // i { aln_ref[ialn] = '-'; aln_seq[ialn] = seq[nseq-i]; ipos = 0; nout_seq++; l -= kd - 1; i--; } else if ( i<=0 || mat[l].dir==-1 ) // d { aln_ref[ialn] = ref[nref-j]; aln_seq[ialn] = '-'; ipos = 0; nout_ref++; l--; j--; } else // match or mismatch { aln_ref[ialn] = ref[nref-j]; aln_seq[ialn] = seq[nseq-i]; ipos = ref[nref-j+1]==seq[nseq-i+1] ? ipos+1 : 1; nout_seq++; nout_ref++; l -= kd; i--; j--; } ialn++; } aln_ref[ialn] = aln_seq[ialn] = 0; fprintf(stderr, "ref: %s\n", ref); fprintf(stderr, "seq: %s\n", seq); fprintf(stderr, "-> %s\n", aln_ref); fprintf(stderr, "-> %s\n", aln_seq); free(aln_ref); free(aln_seq); fprintf(stderr, " "); for (j=0; jref; char *seq = aux->seq; int nref = aux->nref; int nseq = aux->nseq; if ( (nref+1)*(nseq+1) > aux->nmat ) { aux->nmat = (nref+1)*(nseq+1); aux->mat = (cell_t *) realloc(aux->mat, sizeof(cell_t)*aux->nmat); if ( !aux->mat ) error("Could not allocate %ld bytes of memory at %d\n", sizeof(cell_t)*aux->nmat, args->files->readers[0].buffer[0]->pos+1); } const int GAP_OPEN = -1, GAP_CLOSE = -1, GAP_EXT = 0, MATCH = 1, MISM = -1, DI = 1, DD = -1, DM = 0; cell_t *mat = aux->mat; int i, j, k = nref+2, kd = nref+2; mat[0].val = 20; mat[0].dir = DM; // the last ref and alt bases match for (j=1; j<=nref; j++) { mat[j].val = 0; mat[j].dir = DM; } for (i=1; i<=nseq; i++) { mat[k-1].val = 0; mat[k-1].dir = DM; int jmax = i-1 < nref ? i-1 : nref; for (j=1; j<=jmax; j++) { // prefer insertions to deletions and mismatches int max, dir, score; if ( ref[nref-j]==seq[nseq-i] ) { // match max = mat[k-kd].val + MATCH; if ( mat[k-kd].dir!=DM ) max += GAP_CLOSE; dir = DM; // insertion score = mat[k-kd+1].dir == DI ? mat[k-kd+1].val + GAP_EXT: mat[k-kd+1].val + GAP_OPEN; if ( max < score ) { max = score; dir = DI; } // deletion score = mat[k-1].dir == DD ? mat[k-1].val + GAP_EXT : mat[k-1].val + GAP_OPEN; if ( max < score ) { max = score; dir = DD; } } else { // insertion max = mat[k-kd+1].dir == DI ? mat[k-kd+1].val + GAP_EXT : mat[k-kd+1].val + GAP_OPEN; dir = DI; // deletion score = mat[k-1].dir == DD ? mat[k-1].val + GAP_EXT : mat[k-1].val + GAP_OPEN; if ( max < score ) { max = score; dir = DD; } // mismatch score = mat[k-kd].val + MISM; if ( mat[k-kd].dir!=DM ) score += GAP_CLOSE; if ( max < score ) { max = score; dir = DM; } } mat[k].val = max; mat[k].dir = dir; k++; } for (j=jmax+1; j<=nref; j++) { // prefer deletions to insertions and mismatches int max, dir, score; if ( ref[nref-j]==seq[nseq-i] ) { // match max = mat[k-kd].val + MATCH; if ( mat[k-kd].dir!=DM ) max += GAP_CLOSE; dir = DM; // deletion score = mat[k-1].dir == DD ? mat[k-1].val + GAP_EXT: mat[k-1].val + GAP_OPEN; if ( max < score ) { max = score; dir = DD; } // insertion score = mat[k-kd+1].dir == DI ? mat[k-kd+1].val + GAP_EXT : mat[k-kd+1].val + GAP_OPEN; if ( max < score ) { max = score; dir = DI; } } else { // deletion max = mat[k-1].dir == DD ? mat[k-1].val + GAP_EXT : mat[k-1].val + GAP_OPEN; dir = DD; // insertion score = mat[k-kd+1].dir == DI ? mat[k-kd+1].val + GAP_EXT : mat[k-kd+1].val + GAP_OPEN; if ( max < score ) { max = score; dir = DI; } // mismatch score = mat[k-kd].val + MISM; if ( mat[k-kd].dir!=DM ) score += GAP_CLOSE; if ( max < score ) { max = score; dir = DM; } } mat[k].val = max; mat[k].dir = dir; k++; } k++; } // _vcfnorm_debug_print(aux); // Skip as much of the matching sequence at the beggining as possible. (Note, the sequence // is reversed, thus skipping from the end.) k = (nref+1)*(nseq+1)-1; int kmin = nref>nseq ? 2*(nref+1) - nseq : (nseq-nref)*(nref+1); // skip the first row and column of the matrix, which are 0s int ipos = 0; while (k>kmin && mat[k].dir==DM && ref[ipos]==seq[ipos]) { k -= kd; ipos++; } i = k/(nref+1); // seq[nseq-i] j = k - i*(nref+1); // ref[nref-j] if ( !i && !j ) { // no gaps: this is a legitimate case, consider MNPs int l = 0; while ( l0 ); while ( ipos>l && ref[ipos]==seq[ipos] ) ipos--; aux->ipos = l; // position of the last matching base (buffer coordinates) aux->lref = ipos + 1; // position of the first base in the suffix aux->lseq = ipos + 1; return 0; } assert(i>0 && j>0); int l = k, nout_ref = ipos, nout_seq = ipos, nsuffix = 0; while ( l>0 ) { if ( j<=0 || mat[l].dir==DI ) // insertion { nsuffix = 0; nout_seq++; l -= kd - 1; i--; } else if ( i<=0 || mat[l].dir==DD ) // deletion { nsuffix = 0; nout_ref++; l--; j--; } else // match/mismatch { nsuffix = ref[nref-j]==seq[nseq-i] ? nsuffix + 1 : 0; nout_seq++; nout_ref++; l -= kd; i--; j--; } } if ( !ipos ) return -1; // the window is too small aux->ipos = ipos - 1; aux->lref = nout_ref - nsuffix; aux->lseq = nout_seq - nsuffix; // The indels and complex events do not have to be padded if ( aux->lref - aux->ipos > 1 && aux->lseq - aux->ipos > 1 && ref[aux->ipos]==seq[aux->ipos] ) aux->ipos++; return 0; } int realign(args_t *args, bcf1_t *line) { bcf_unpack(line, BCF_UN_STR); char *tmp; int i, ref_len = strlen(line->d.allele[0]), len = ref_len; for (i=1; in_allele; i++) { int l = strlen(line->d.allele[i]); if ( len < l ) len = l; } for (i=0; in_allele; i++) { tmp = line->d.allele[i]; while (*tmp) { *tmp = toupper(*tmp); tmp++; } } int ref_winlen; char *ref = NULL; if ( len==1 || line->n_allele==1 ) { // SNP int reflen = strlen(line->d.allele[0]); ref = faidx_fetch_seq(args->fai, (char*)args->hdr->id[BCF_DT_CTG][line->rid].key, line->pos, line->pos+reflen-1, &ref_winlen); if ( !ref ) error("faidx_fetch_seq failed at %s:%d\n", args->hdr->id[BCF_DT_CTG][line->rid].key, line->pos+1); if ( !strncmp(ref,line->d.allele[0],reflen) ) { free(ref); return 0; } if ( args->check_ref==CHECK_REF_EXIT ) error("Reference allele mismatch at %s:%d .. '%c' vs '%c'\n", bcf_seqname(args->hdr,line),line->pos+1,ref[0],line->d.allele[0][0]); if ( args->check_ref & CHECK_REF_WARN ) fprintf(stderr,"REF_MISMATCH\t%s\t%d\t%s\n", bcf_seqname(args->hdr,line),line->pos+1,line->d.allele[0]); free(ref); return -1; } // Sanity check: exclude broken VCFs with long stretches of N's if ( len>1000 ) { for (i=0; id.allele[0][i]=='N' ) return -1; } int win = line->pos < args->aln_win ? line->pos : args->aln_win; len += win + 2; if ( args->mseq < len*(line->n_allele-1) ) { args->mseq = len*(line->n_allele-1); args->seq = (char*) realloc(args->seq, sizeof(char)*args->mseq); } ref = faidx_fetch_seq(args->fai, (char*)args->hdr->id[BCF_DT_CTG][line->rid].key, line->pos-win, line->pos+ref_len, &ref_winlen); if ( !ref ) error("faidx_fetch_seq failed at %s:%d\n", args->hdr->id[BCF_DT_CTG][line->rid].key, line->pos-win); assert( ref_winlen==ref_len+win+1 ); for (i=0; id.allele[0],ref_len) ) { for (i=0; id.allele[0][i] ) break; if ( args->check_ref==CHECK_REF_EXIT ) error("\nSanity check failed, the reference sequence differs at %s:%d[%d] .. '%c' vs '%c'\n", args->hdr->id[BCF_DT_CTG][line->rid].key, line->pos+1, i+1,ref[win+i],line->d.allele[0][i]); if ( args->check_ref & CHECK_REF_WARN ) fprintf(stderr,"REF_MISMATCH\t%s\t%d\t%s\n", bcf_seqname(args->hdr,line),line->pos+1,line->d.allele[0]); free(ref); return -1; } if ( args->aln.m_arr < line->n_allele ) { args->aln.m_arr = line->n_allele; args->aln.ipos_arr = (int*) realloc(args->aln.ipos_arr, sizeof(int)*args->aln.m_arr); args->aln.lref_arr = (int*) realloc(args->aln.lref_arr, sizeof(int)*args->aln.m_arr); args->aln.lseq_arr = (int*) realloc(args->aln.lseq_arr, sizeof(int)*args->aln.m_arr); } int *ipos = args->aln.ipos_arr; int *iref = args->aln.lref_arr; int *iseq = args->aln.lseq_arr; int min_pos = INT_MAX, max_ref = 0; static int aln_win_warned = 0; int j, k; for (j=1; jn_allele; j++) { // get the ALT ready for alignment args->tseq = args->seq + (j-1)*len; for (i=0; itseq[i] = ref[i]; char *t = line->d.allele[j]; while (*t) { args->tseq[i++] = *t; t++; } args->tseq[i++] = ref[ref_winlen-1]; args->tseq[i] = 0; args->aln.ref = ref; args->aln.seq = args->tseq; args->aln.nref = ref_winlen; // reflen which goes into realignment: VCF ref + #win bases which precede args->aln.nseq = i; // same as reflen but for alt int ret = align(args, &args->aln); if ( ret<0 ) { free(ref); if ( ret==-1 ) { // todo: better error analysis, see 2:1 in test/norm.vcf // fprintf(stderr,"Warning: The -w alignment window too small for %s:%d\n", bcf_seqname(args->hdr,line),line->pos+1); return 0; // leave as is } if ( ret==-2 ) fprintf(stderr,"Warning: REF allele is identical to ALT at %s:%d\n", bcf_seqname(args->hdr,line),line->pos+1); return -1; } #if 0 fprintf(stderr, "%s \t nref=%d\n", ref, ref_winlen); fprintf(stderr, "%s \t nseq=%d\n", args->tseq, i); fprintf(stderr, "pos=%d win=%d ipos=%d lref=%d lseq=%d\n", line->pos+1, win, args->aln.ipos, args->aln.lref, args->aln.lseq); fprintf(stderr, "-> "); for (k=args->aln.ipos; kaln.lref; k++) fprintf(stderr, "%c", ref[k]); fprintf(stderr, "\n"); fprintf(stderr, "-> "); for (k=args->aln.ipos; kaln.lseq; k++) fprintf(stderr, "%c", args->tseq[k]); fprintf(stderr, "\n"); fprintf(stderr, "\n"); #endif if ( !args->aln.ipos && !aln_win_warned ) { fprintf(stderr,"Warning: bigger -w is needed [todo: improve me, %s:%d %s,%s]\n", bcf_seqname(args->hdr,line),line->pos+1, line->d.allele[0],line->d.allele[j]); aln_win_warned = 1; } ipos[j] = args->aln.ipos; // 0-based position before the first difference (w.r.t. the window) iref[j] = args->aln.lref; // length of the REF alignment (or the index after the last aligned position) iseq[j] = args->aln.lseq; if ( max_ref < iref[j] ) max_ref = iref[j]; if ( min_pos > ipos[j] ) min_pos = ipos[j]; assert( iseq[j]<=len ); } // Check if the record's position must be changed int nmv = win - min_pos; // This assertion that we will never want align more to the right is too // strong, consider cases like GATG -> GACT // assert( nmv>=0 ); line->pos -= nmv; hts_expand0(kstring_t,line->n_allele,args->ntmp_als,args->tmp_als); // REF args->tmp_als[0].l = 0; kputsn(&ref[min_pos], max_ref-min_pos, &args->tmp_als[0]); // ALTs int min_len = args->tmp_als[0].l; for (k=1; kn_allele; k++) { args->tmp_als[k].l = 0; // prefix the sequence with REF bases if the other alleles were aligned more to the left int nprefix = ipos[k] - min_pos; if ( nprefix ) kputsn(&ref[min_pos], nprefix, &args->tmp_als[k]); // the ALT sequence int nseq = iseq[k] - ipos[k]; if ( nseq ) { char *alt = args->seq + (k-1)*len + ipos[k]; kputsn(alt, nseq, &args->tmp_als[k]); } // suffix invoked by other deletions which must be added to match the REF int nsuffix = max_ref - iref[k]; if ( nsuffix ) kputsn(&ref[iref[k]], nsuffix, &args->tmp_als[k]); if ( min_len > args->tmp_als[k].l ) min_len = args->tmp_als[k].l; } free(ref); // create new block of alleles args->tmp_als_str.l = 0; if ( args->parsimonious ) { // check if we can trim from the left for (i=0; itmp_als[0].l; i++) { for (k=1; kn_allele; k++) if ( args->tmp_als[0].s[i]!=args->tmp_als[k].s[i] ) break; if ( k!=line->n_allele ) break; } if ( i>=min_len ) i = min_len - 1; if ( i>0 ) // can be left trimmed { for (k=0; kn_allele; k++) { if (k>0) kputc(',',&args->tmp_als_str); kputsn(args->tmp_als[k].s+i,args->tmp_als[k].l-i,&args->tmp_als_str); } kputc(0,&args->tmp_als_str); line->pos += i; } // if REF allele has not changed, ALTs must be unchanged as well else if ( !strcmp(line->d.allele[0],args->tmp_als[0].s) ) return 1; } else if ( !strcmp(line->d.allele[0],args->tmp_als[0].s) ) return 1; if ( !args->tmp_als_str.l ) { for (k=0; kn_allele; k++) { if (k>0) kputc(',',&args->tmp_als_str); kputsn(args->tmp_als[k].s,args->tmp_als[k].l,&args->tmp_als_str); } } args->nchanged++; bcf_update_alleles_str(args->hdr,line,args->tmp_als_str.s); return 1; } #else static int realign(args_t *args, bcf1_t *line) { bcf_unpack(line, BCF_UN_STR); // Sanity check REF int nref, reflen = strlen(line->d.allele[0]); char *ref = faidx_fetch_seq(args->fai, (char*)args->hdr->id[BCF_DT_CTG][line->rid].key, line->pos, line->pos+reflen-1, &nref); if ( !ref ) error("faidx_fetch_seq failed at %s:%d\n", args->hdr->id[BCF_DT_CTG][line->rid].key, line->pos+1); if ( strcasecmp(ref,line->d.allele[0]) ) { if ( args->check_ref==CHECK_REF_EXIT ) error("Reference allele mismatch at %s:%d .. '%s' vs '%s'\n", bcf_seqname(args->hdr,line),line->pos+1,ref,line->d.allele[0]); if ( args->check_ref & CHECK_REF_WARN ) fprintf(stderr,"REF_MISMATCH\t%s\t%d\t%s\n", bcf_seqname(args->hdr,line),line->pos+1,line->d.allele[0]); free(ref); return -1; } free(ref); ref = NULL; if ( line->n_allele == 1 ) return 0; // a REF // make a copy of each allele for trimming int i; hts_expand0(kstring_t,line->n_allele,args->ntmp_als,args->tmp_als); kstring_t *als = args->tmp_als; for (i=0; in_allele; i++) { if ( line->d.allele[i][0]=='<' ) return 0; // symbolic allele als[i].l = 0; kputs(line->d.allele[i], &als[i]); } // trim from right int ori_pos = line->pos; while (1) { // is the rightmost base identical in all alleles? for (i=1; in_allele; i++) { if ( als[0].s[ als[0].l-1 ]!=als[i].s[ als[i].l-1 ] ) break; } if ( i!=line->n_allele ) break; // there are differences, cannot be trimmed int pad_from_left = 0; for (i=0; in_allele; i++) // trim all alleles { als[i].l--; if ( !als[i].l ) pad_from_left = 1; } if ( pad_from_left ) { int npad = line->pos >= args->aln_win ? args->aln_win : line->pos; free(ref); ref = faidx_fetch_seq(args->fai, (char*)args->hdr->id[BCF_DT_CTG][line->rid].key, line->pos-npad, line->pos-1, &nref); if ( !ref ) error("faidx_fetch_seq failed at %s:%d\n", args->hdr->id[BCF_DT_CTG][line->rid].key, line->pos-npad+1); for (i=0; in_allele; i++) { ks_resize(&als[i], als[i].l + npad); if ( als[i].l ) memmove(als[i].s+npad,als[i].s,als[i].l); memcpy(als[i].s,ref,npad); als[i].l += npad; } line->pos -= npad; } } free(ref); // trim from left int ntrim_left = 0; while (1) { // is the first base identical in all alleles? int min_len = als[0].l - ntrim_left; for (i=1; in_allele; i++) { if ( als[0].s[ntrim_left]!=als[i].s[ntrim_left] ) break; if ( min_len > als[i].l - ntrim_left ) min_len = als[i].l - ntrim_left; } if ( i!=line->n_allele || min_len==1 ) break; // there are differences, cannot be trimmed ntrim_left++; } if ( ntrim_left ) { for (i=0; in_allele; i++) { memmove(als[i].s,als[i].s+ntrim_left,als[i].l-ntrim_left); als[i].l -= ntrim_left; } line->pos += ntrim_left; } // Have the alleles changed? als[0].s[ als[0].l ] = 0; // in order for strcmp to work if ( ori_pos==line->pos && !strcasecmp(line->d.allele[0],als[0].s) ) return 1; // Create new block of alleles and update args->tmp_als_str.l = 0; for (i=0; in_allele; i++) { if (i>0) kputc(',',&args->tmp_als_str); kputsn(als[i].s,als[i].l,&args->tmp_als_str); } args->tmp_als_str.s[ args->tmp_als_str.l ] = 0; bcf_update_alleles_str(args->hdr,line,args->tmp_als_str.s); args->nchanged++; return 1; } #endif static void split_info_numeric(args_t *args, bcf1_t *src, bcf_info_t *info, int ialt, bcf1_t *dst) { #define BRANCH_NUMERIC(type,type_t) \ { \ const char *tag = bcf_hdr_int2id(args->hdr,BCF_DT_ID,info->key); \ int ntmp = args->ntmp_arr1 / sizeof(type_t); \ int ret = bcf_get_info_##type(args->hdr,src,tag,&args->tmp_arr1,&ntmp); \ args->ntmp_arr1 = ntmp * sizeof(type_t); \ assert( ret>0 ); \ type_t *vals = (type_t*) args->tmp_arr1; \ int len = bcf_hdr_id2length(args->hdr,BCF_HL_INFO,info->key); \ if ( len==BCF_VL_A ) \ { \ assert( ret==src->n_allele-1); \ bcf_update_info_##type(args->hdr,dst,tag,vals+ialt,1); \ } \ else if ( len==BCF_VL_R ) \ { \ assert( ret==src->n_allele); \ if ( ialt!=0 ) vals[1] = vals[ialt+1]; \ bcf_update_info_##type(args->hdr,dst,tag,vals,2); \ } \ else if ( len==BCF_VL_G ) \ { \ assert( ret==src->n_allele*(src->n_allele+1)/2 ); \ if ( ialt!=0 ) \ { \ vals[1] = vals[bcf_alleles2gt(0,ialt+1)]; \ vals[2] = vals[bcf_alleles2gt(ialt+1,ialt+1)]; \ } \ bcf_update_info_##type(args->hdr,dst,tag,vals,3); \ } \ else \ bcf_update_info_##type(args->hdr,dst,tag,vals,ret); \ } switch (bcf_hdr_id2type(args->hdr,BCF_HL_INFO,info->key)) { case BCF_HT_INT: BRANCH_NUMERIC(int32, int32_t); break; case BCF_HT_REAL: BRANCH_NUMERIC(float, float); break; } #undef BRANCH_NUMERIC } // Find n-th field in a comma-separated list and move it to dst. // The memory areas may overlap. #define STR_MOVE_NTH(dst,src,end,nth,len) \ { \ char *ss = src, *se = src; \ int j = 0; \ while ( *se && se<(end) ) \ { \ if ( *se==',' ) \ { \ if ( j==nth ) break; \ j++; \ ss = se+1; \ } \ se++; \ } \ if ( j==nth ) \ { \ int n = se - ss; \ memmove((dst),ss,n); \ src = se; \ len += n; \ } \ else len = -1; \ } static void split_info_string(args_t *args, bcf1_t *src, bcf_info_t *info, int ialt, bcf1_t *dst) { const char *tag = bcf_hdr_int2id(args->hdr,BCF_DT_ID,info->key); int ret = bcf_get_info_string(args->hdr,src,tag,&args->tmp_arr1,&args->ntmp_arr1); assert( ret>0 ); kstring_t str; str.m = args->ntmp_arr1; str.l = ret; str.s = (char*) args->tmp_arr1; int len = bcf_hdr_id2length(args->hdr,BCF_HL_INFO,info->key); if ( len==BCF_VL_A ) { char *tmp = str.s; int len = 0; STR_MOVE_NTH(str.s,tmp,str.s+str.l,ialt,len); if ( len<0 ) return; // wrong number of fields: skip str.s[len] = 0; bcf_update_info_string(args->hdr,dst,tag,str.s); } else if ( len==BCF_VL_R ) { char *tmp = str.s; int len = 0; STR_MOVE_NTH(str.s,tmp,str.s+str.l,0,len); str.s[len]=','; tmp++; len++; STR_MOVE_NTH(&str.s[len],tmp,str.s+str.l,ialt,len); if ( len<0 ) return; // wrong number of fields: skip str.s[len] = 0; bcf_update_info_string(args->hdr,dst,tag,str.s); } else if ( len==BCF_VL_G ) { int i0a = bcf_alleles2gt(0,ialt+1), iaa = bcf_alleles2gt(ialt+1,ialt+1); char *tmp = str.s; int len = 0; STR_MOVE_NTH(str.s,tmp,str.s+str.l,0,len); str.s[len]=','; tmp++; len++; STR_MOVE_NTH(&str.s[len],tmp,str.s+str.l,i0a-1,len); if ( len<0 ) return; // wrong number of fields: skip str.s[len]=','; tmp++; len++; STR_MOVE_NTH(&str.s[len],tmp,str.s+str.l,iaa-i0a-1,len); if ( len<0 ) return; // wrong number of fields: skip str.s[len] = 0; bcf_update_info_string(args->hdr,dst,tag,str.s); } else bcf_update_info_string(args->hdr,dst,tag,str.s); } static void split_info_flag(args_t *args, bcf1_t *src, bcf_info_t *info, int ialt, bcf1_t *dst) { const char *tag = bcf_hdr_int2id(args->hdr,BCF_DT_ID,info->key); int ret = bcf_get_info_flag(args->hdr,src,tag,&args->tmp_arr1,&args->ntmp_arr1); bcf_update_info_flag(args->hdr,dst,tag,NULL,ret); } static void split_format_genotype(args_t *args, bcf1_t *src, bcf_fmt_t *fmt, int ialt, bcf1_t *dst) { int ntmp = args->ntmp_arr1 / 4; int ngts = bcf_get_genotypes(args->hdr,src,&args->tmp_arr1,&ntmp); args->ntmp_arr1 = ntmp * 4; assert( ngts >0 ); int32_t *gt = (int32_t*) args->tmp_arr1; int i, j, nsmpl = bcf_hdr_nsamples(args->hdr); ngts /= nsmpl; for (i=0; ihdr,dst,args->tmp_arr1,ngts*nsmpl); } static void split_format_numeric(args_t *args, bcf1_t *src, bcf_fmt_t *fmt, int ialt, bcf1_t *dst) { #define BRANCH_NUMERIC(type,type_t,is_vector_end,set_vector_end) \ { \ const char *tag = bcf_hdr_int2id(args->hdr,BCF_DT_ID,fmt->id); \ int ntmp = args->ntmp_arr1 / sizeof(type_t); \ int nvals = bcf_get_format_##type(args->hdr,src,tag,&args->tmp_arr1,&ntmp); \ args->ntmp_arr1 = ntmp * sizeof(type_t); \ assert( nvals>0 ); \ type_t *vals = (type_t *) args->tmp_arr1; \ int len = bcf_hdr_id2length(args->hdr,BCF_HL_FMT,fmt->id); \ int i, nsmpl = bcf_hdr_nsamples(args->hdr); \ if ( nvals==nsmpl ) /* all values are missing */ \ { \ bcf_update_format_##type(args->hdr,dst,tag,vals,nsmpl); \ return; \ } \ if ( len==BCF_VL_A ) \ { \ assert( nvals==(src->n_allele-1)*nsmpl); \ nvals /= nsmpl; \ type_t *src_vals = vals, *dst_vals = vals; \ for (i=0; ihdr,dst,tag,vals,nsmpl); \ } \ else if ( len==BCF_VL_R ) \ { \ assert( nvals==src->n_allele*nsmpl); \ nvals /= nsmpl; \ type_t *src_vals = vals, *dst_vals = vals; \ for (i=0; ihdr,dst,tag,vals,nsmpl*2); \ } \ else if ( len==BCF_VL_G ) \ { \ if ( nvals!=src->n_allele*(src->n_allele+1)/2*nsmpl && nvals!=src->n_allele*nsmpl ) \ error("Error at %s:%d, the tag %s has wrong number of fields\n", bcf_seqname(args->hdr,src),src->pos+1,bcf_hdr_int2id(args->hdr,BCF_DT_ID,fmt->id)); \ nvals /= nsmpl; \ int all_haploid = nvals==src->n_allele ? 1 : 0; \ type_t *src_vals = vals, *dst_vals = vals; \ for (i=0; ihdr,dst,tag,vals,all_haploid ? nsmpl*2 : nsmpl*3); \ } \ else \ bcf_update_format_##type(args->hdr,dst,tag,vals,nvals); \ } switch (bcf_hdr_id2type(args->hdr,BCF_HL_FMT,fmt->id)) { case BCF_HT_INT: BRANCH_NUMERIC(int32, int32_t, src_vals[j]==bcf_int32_vector_end, dst_vals[2]=bcf_int32_vector_end); break; case BCF_HT_REAL: BRANCH_NUMERIC(float, float, bcf_float_is_vector_end(src_vals[j]), bcf_float_set_vector_end(dst_vals[2])); break; } #undef BRANCH_NUMERIC } static void squeeze_format_char(char *str, int src_blen, int dst_blen, int n) { int i, isrc = 0, idst = 0; for (i=0; ihdr,BCF_DT_ID,fmt->id); int ret = bcf_get_format_char(args->hdr,src,tag,&args->tmp_arr1,&args->ntmp_arr1); assert( ret>0 ); kstring_t str; str.m = args->ntmp_arr1; str.l = ret; str.s = (char*) args->tmp_arr1; int nsmpl = bcf_hdr_nsamples(args->hdr); int len = bcf_hdr_id2length(args->hdr,BCF_HL_FMT,fmt->id); if ( len==BCF_VL_A ) { int i, blen = ret/nsmpl, maxlen = 0; char *ptr = str.s; for (i=0; ihdr,dst,tag,str.s,nsmpl*maxlen); } else if ( len==BCF_VL_R ) { int i, blen = ret/nsmpl, maxlen = 0; char *ptr = str.s; for (i=0; ihdr,dst,tag,str.s,nsmpl*maxlen); } else if ( len==BCF_VL_G ) { int i, blen = ret/nsmpl, maxlen = 0, i0a = bcf_alleles2gt(0,ialt+1), iaa = bcf_alleles2gt(ialt+1,ialt+1); char *ptr = str.s; for (i=0; in_allele*(src->n_allele+1)/2 || nfields==src->n_allele ); int len = 0; if ( nfields==src->n_allele ) // haploid { char *tmp = ptr; STR_MOVE_NTH(&ptr[len],tmp,ptr+blen,0,len); ptr[len]=','; tmp++; len++; STR_MOVE_NTH(&ptr[len],tmp,ptr+blen,ialt,len); if ( len<0 ) return; // wrong number of fields: skip } else // diploid { char *tmp = ptr; STR_MOVE_NTH(&ptr[len],tmp,ptr+blen,0,len); ptr[len]=','; tmp++; len++; STR_MOVE_NTH(&ptr[len],tmp,ptr+blen,i0a-1,len); if ( len<0 ) return; // wrong number of fields: skip ptr[len]=','; tmp++; len++; STR_MOVE_NTH(&ptr[len],tmp,ptr+blen,iaa-i0a-1,len); if ( len<0 ) return; // wrong number of fields: skip } if ( maxlen < len ) maxlen = len; ptr += blen; } if ( maxlenhdr,dst,tag,str.s,nsmpl*maxlen); } else bcf_update_format_char(args->hdr,dst,tag,str.s,str.l); } static void split_multiallelic_to_biallelics(args_t *args, bcf1_t *line) { int i; bcf_unpack(line, BCF_UN_ALL); // Init the target biallelic lines args->ntmp_lines = line->n_allele-1; if ( args->mtmp_lines < args->ntmp_lines ) { args->tmp_lines = (bcf1_t **)realloc(args->tmp_lines,sizeof(bcf1_t*)*args->ntmp_lines); for (i=args->mtmp_lines; intmp_lines; i++) args->tmp_lines[i] = bcf_init1(); args->mtmp_lines = args->ntmp_lines; } kstring_t tmp = {0,0,0}; kputs(line->d.allele[0], &tmp); kputc(',', &tmp); int rlen = tmp.l; int gt_id = bcf_hdr_id2int(args->hdr,BCF_DT_ID,"GT"); for (i=0; intmp_lines; i++) // for each ALT allele { bcf1_t *dst = args->tmp_lines[i]; bcf_clear(dst); dst->rid = line->rid; dst->pos = line->pos; dst->qual = line->qual; tmp.l = rlen; kputs(line->d.allele[i+1],&tmp); bcf_update_alleles_str(args->hdr,dst,tmp.s); if ( line->d.n_flt ) bcf_update_filter(args->hdr, dst, line->d.flt, line->d.n_flt); int j; for (j=0; jn_info; j++) { bcf_info_t *info = &line->d.info[j]; int type = bcf_hdr_id2type(args->hdr,BCF_HL_INFO,info->key); if ( type==BCF_HT_INT || type==BCF_HT_REAL ) split_info_numeric(args, line, info, i, dst); else if ( type==BCF_HT_FLAG ) split_info_flag(args, line, info, i, dst); else split_info_string(args, line, info, i, dst); } dst->n_sample = line->n_sample; for (j=0; jn_fmt; j++) { bcf_fmt_t *fmt = &line->d.fmt[j]; int type = bcf_hdr_id2type(args->hdr,BCF_HL_FMT,fmt->id); if ( fmt->id==gt_id ) split_format_genotype(args, line, fmt, i, dst); else if ( type==BCF_HT_INT || type==BCF_HT_REAL ) split_format_numeric(args, line, fmt, i, dst); else split_format_string(args, line, fmt, i, dst); } } free(tmp.s); } // Enlarge FORMAT array containing nsmpl samples each with nals_ori values // to accommodate nvals values for each sample, filling the gaps with missing // values. Works also for INFO arrays, with nsmpl set to 1. #define ENLARGE_ARRAY(type_t,set_missing,arr,narr_bytes,nsmpl,nvals_ori,nvals) \ { \ int nbytes_new = (nsmpl)*(nvals)*sizeof(type_t); \ hts_expand(uint8_t,nbytes_new,narr_bytes,arr); \ int ismpl, k; \ for (ismpl=nsmpl-1; ismpl>=0; ismpl--) \ { \ type_t *dst_ptr = ((type_t*)arr) + ismpl*(nvals); \ type_t *src_ptr = ((type_t*)arr) + ismpl*nvals_ori; \ memmove(dst_ptr,src_ptr,sizeof(type_t)*nvals_ori); \ for (k=nvals_ori; khdr,BCF_DT_ID,info->key); \ int ntmp = args->ntmp_arr1 / sizeof(type_t); \ int nvals_ori = bcf_get_info_##type(args->hdr,lines[0],tag,&args->tmp_arr1,&ntmp); \ args->ntmp_arr1 = ntmp * sizeof(type_t); \ assert( nvals_ori>0 ); \ type_t *vals = (type_t*) args->tmp_arr1, *vals2; \ int i,k,len = bcf_hdr_id2length(args->hdr,BCF_HL_INFO,info->key); \ if ( len==BCF_VL_A ) \ { \ assert( nvals_ori==lines[0]->n_allele - 1); \ int nvals = dst->n_allele - 1; \ ENLARGE_ARRAY(type_t,set_missing,args->tmp_arr1,args->ntmp_arr1,1,nvals_ori,nvals); \ vals = (type_t*) args->tmp_arr1; \ for (i=1; intmp_arr2 / sizeof(type_t); \ int nvals2 = bcf_get_info_##type(args->hdr,lines[i],tag,&args->tmp_arr2,&ntmp2); \ args->ntmp_arr2 = ntmp2 * sizeof(type_t); \ assert( nvals2==lines[i]->n_allele-1 ); \ vals2 = (type_t*) args->tmp_arr2; \ for (k=0; kmaps[i].map[k+1] - 1 ] = vals2[k]; \ } \ } \ bcf_update_info_##type(args->hdr,dst,tag,args->tmp_arr1,nvals); \ } \ else if ( len==BCF_VL_R ) \ { \ assert( nvals_ori==lines[0]->n_allele ); \ int nvals = dst->n_allele; \ ENLARGE_ARRAY(type_t,set_missing,args->tmp_arr1,args->ntmp_arr1,1,nvals_ori,nvals); \ vals = (type_t*) args->tmp_arr1; \ for (i=1; intmp_arr2 / sizeof(type_t); \ int nvals2 = bcf_get_info_##type(args->hdr,lines[i],tag,&args->tmp_arr2,&ntmp2); \ args->ntmp_arr2 = ntmp2 * sizeof(type_t); \ assert( nvals2==lines[i]->n_allele ); \ vals2 = (type_t*) args->tmp_arr2; \ for (k=0; kmaps[i].map[k] ] = vals2[k]; \ } \ } \ bcf_update_info_##type(args->hdr,dst,tag,args->tmp_arr1,nvals); \ } \ else if ( len==BCF_VL_G ) \ { \ assert( nvals_ori==lines[0]->n_allele*(lines[0]->n_allele+1)/2 ); /* expecting diploid gt in INFO */ \ int nvals = dst->n_allele*(dst->n_allele+1)/2; \ ENLARGE_ARRAY(type_t,set_missing,args->tmp_arr1,args->ntmp_arr1,1,nvals_ori,nvals); \ vals = (type_t*) args->tmp_arr1; \ for (i=1; intmp_arr2 / sizeof(type_t); \ int nvals2 = bcf_get_info_##type(args->hdr,lines[i],tag,&args->tmp_arr2,&ntmp2); \ args->ntmp_arr2 = ntmp2 * sizeof(type_t); \ assert( nvals2==lines[i]->n_allele*(lines[i]->n_allele+1)/2 ); \ vals2 = (type_t*) args->tmp_arr2; \ int ia,ib; \ k = 0; \ for (ia=0; ian_allele; ia++) \ { \ for (ib=0; ib<=ia; ib++) \ { \ if ( is_vector_end ) break; \ int l = bcf_alleles2gt(args->maps[i].map[ia],args->maps[i].map[ib]); \ vals[l] = vals2[k]; \ k++; \ } \ } \ } \ bcf_update_info_##type(args->hdr,dst,tag,args->tmp_arr1,nvals); \ } \ else \ bcf_update_info_##type(args->hdr,dst,tag,vals,nvals_ori); \ } switch (bcf_hdr_id2type(args->hdr,BCF_HL_INFO,info->key)) { case BCF_HT_INT: BRANCH_NUMERIC(int32, int32_t, dst_ptr[k]=bcf_int32_missing, vals2[k]==bcf_int32_vector_end); break; case BCF_HT_REAL: BRANCH_NUMERIC(float, float, bcf_float_set_missing(dst_ptr[k]), bcf_float_is_vector_end(vals2[k])); break; } #undef BRANCH_NUMERIC } static void merge_info_flag(args_t *args, bcf1_t **lines, int nlines, bcf_info_t *info, bcf1_t *dst) { const char *tag = bcf_hdr_int2id(args->hdr,BCF_DT_ID,info->key); int ret = bcf_get_info_flag(args->hdr,lines[0],tag,&args->tmp_arr1,&args->ntmp_arr1); bcf_update_info_flag(args->hdr,dst,tag,NULL,ret); } int copy_string_field(char *src, int isrc, int src_len, kstring_t *dst, int idst); // see vcfmerge.c static void merge_info_string(args_t *args, bcf1_t **lines, int nlines, bcf_info_t *info, bcf1_t *dst) { const char *tag = bcf_hdr_int2id(args->hdr,BCF_DT_ID,info->key); kstring_t str; str.m = args->ntmp_arr1; str.l = 0; str.s = (char*) args->tmp_arr1; int i, j, len = bcf_hdr_id2length(args->hdr,BCF_HL_INFO,info->key); if ( len==BCF_VL_A || len==BCF_VL_R ) { int jfrom = len==BCF_VL_A ? 1 : 0; kputc('.',&str); for (i=jfrom+1; in_allele; i++) kputs(",.",&str); for (i=0; ihdr,lines[i],tag); for (j=jfrom; jn_allele; j++) copy_string_field((char*)src->vptr, j-jfrom, src->len, &str, args->maps[i].map[j]-jfrom); } str.s[str.l] = 0; args->tmp_arr1 = (uint8_t*) str.s; args->ntmp_arr1 = str.m; bcf_update_info_string(args->hdr,dst,tag,str.s); } else if ( len==BCF_VL_G ) { int ngts = dst->n_allele*(dst->n_allele+1)/2; kputc('.',&str); for (i=1; ihdr,lines[i],tag); int iori, jori, kori = 0; for (iori=0; iorin_allele; iori++) { int inew = args->maps[i].map[iori]; for (jori=0; jori<=iori; jori++) { int jnew = args->maps[i].map[jori]; int knew = bcf_alleles2gt(inew,jnew); copy_string_field((char*)src->vptr,kori,src->len,&str,knew); kori++; } } } str.s[str.l] = 0; args->tmp_arr1 = (uint8_t*) str.s; args->ntmp_arr1 = str.m; bcf_update_info_string(args->hdr,dst,tag,str.s); } else { bcf_get_info_string(args->hdr,lines[0],tag,&args->tmp_arr1,&args->ntmp_arr1); bcf_update_info_string(args->hdr,dst,tag,args->tmp_arr1); } } static void merge_format_genotype(args_t *args, bcf1_t **lines, int nlines, bcf_fmt_t *fmt, bcf1_t *dst) { int ntmp = args->ntmp_arr1 / 4; int ngts = bcf_get_genotypes(args->hdr,lines[0],&args->tmp_arr1,&ntmp); args->ntmp_arr1 = ntmp * 4; assert( ngts >0 ); int nsmpl = bcf_hdr_nsamples(args->hdr); ngts /= nsmpl; int i, j, k; for (i=1; intmp_arr2 / 4; int ngts2 = bcf_get_genotypes(args->hdr,lines[i],&args->tmp_arr2,&ntmp2); args->ntmp_arr2 = ntmp2 * 4; ngts2 /= nsmpl; assert( ngts==ngts2 ); int32_t *gt = (int32_t*) args->tmp_arr1; int32_t *gt2 = (int32_t*) args->tmp_arr2; for (j=0; jmaps[i].nals ); gt[k] = bcf_gt_unphased( args->maps[i].map[ial] ) | bcf_gt_is_phased(gt[k]); } } gt += ngts; gt2 += ngts; } } bcf_update_genotypes(args->hdr,dst,args->tmp_arr1,ngts*nsmpl); } static void merge_format_numeric(args_t *args, bcf1_t **lines, int nlines, bcf_fmt_t *fmt, bcf1_t *dst) { #define BRANCH_NUMERIC(type,type_t,set_missing,is_vector_end) \ { \ const char *tag = bcf_hdr_int2id(args->hdr,BCF_DT_ID,fmt->id); \ int ntmp = args->ntmp_arr1 / sizeof(type_t); \ int nvals_ori = bcf_get_format_##type(args->hdr,lines[0],tag,&args->tmp_arr1,&ntmp); \ args->ntmp_arr1 = ntmp * sizeof(type_t); \ assert( nvals_ori>0 ); \ type_t *vals2, *vals = (type_t *) args->tmp_arr1; \ int len = bcf_hdr_id2length(args->hdr,BCF_HL_FMT,fmt->id); \ int i, j, k, nsmpl = bcf_hdr_nsamples(args->hdr); \ nvals_ori /= nsmpl; \ if ( len==BCF_VL_A ) \ { \ int nvals = dst->n_allele - 1; \ ENLARGE_ARRAY(type_t,set_missing,args->tmp_arr1,args->ntmp_arr1,nsmpl,nvals_ori,nvals); \ for (i=1; intmp_arr2 / sizeof(type_t); \ int nvals2 = bcf_get_format_##type(args->hdr,lines[i],tag,&args->tmp_arr2,&ntmp2); \ args->ntmp_arr2 = ntmp2 * sizeof(type_t); \ nvals2 /= nsmpl; \ assert( nvals2==lines[i]->n_allele-1 ); \ vals = (type_t*) args->tmp_arr1; \ vals2 = (type_t*) args->tmp_arr2; \ for (j=0; jmaps[i].map[k+1] - 1 ] = vals2[k]; \ } \ vals += nvals; \ vals2 += nvals2; \ } \ } \ bcf_update_format_##type(args->hdr,dst,tag,args->tmp_arr1,nvals*nsmpl); \ } \ else if ( len==BCF_VL_R ) \ { \ int nvals = dst->n_allele; \ ENLARGE_ARRAY(type_t,set_missing,args->tmp_arr1,args->ntmp_arr1,nsmpl,nvals_ori,nvals); \ for (i=1; intmp_arr2 / sizeof(type_t); \ int nvals2 = bcf_get_format_##type(args->hdr,lines[i],tag,&args->tmp_arr2,&ntmp2); \ args->ntmp_arr2 = ntmp2 * sizeof(type_t); \ nvals2 /= nsmpl; \ assert( nvals2==lines[i]->n_allele ); \ vals = (type_t*) args->tmp_arr1; \ vals2 = (type_t*) args->tmp_arr2; \ for (j=0; jmaps[i].map[k] ] = vals2[k]; \ } \ vals += nvals; \ vals2 += nvals2; \ } \ } \ bcf_update_format_##type(args->hdr,dst,tag,args->tmp_arr1,nvals*nsmpl); \ } \ else if ( len==BCF_VL_G ) \ { \ int all_haploid = nvals_ori==lines[0]->n_allele ? 1 : 0; \ int nvals = all_haploid ? dst->n_allele : dst->n_allele*(dst->n_allele+1)/2; \ ENLARGE_ARRAY(type_t,set_missing,args->tmp_arr1,args->ntmp_arr1,nsmpl,nvals_ori,nvals); \ for (i=1; intmp_arr2 / sizeof(type_t); \ int nvals2 = bcf_get_format_##type(args->hdr,lines[i],tag,&args->tmp_arr2,&ntmp2); \ args->ntmp_arr2 = ntmp2 * sizeof(type_t); \ nvals2 /= nsmpl; \ assert( nvals2==lines[i]->n_allele || nvals2==lines[i]->n_allele*(lines[i]->n_allele+1)/2); \ vals = (type_t*) args->tmp_arr1; \ vals2 = (type_t*) args->tmp_arr2; \ for (j=0; jmaps[i].map[k] ] = vals2[k]; \ } \ } \ else \ { \ k = 0; \ int ia,ib; \ for (ia=0; ian_allele; ia++) \ { \ for (ib=0; ib<=ia; ib++) \ { \ int l = bcf_alleles2gt(args->maps[i].map[ia],args->maps[i].map[ib]); \ vals[l] = vals2[k]; \ k++; \ } \ } \ } \ vals += nvals; \ vals2 += nvals2; \ } \ } \ bcf_update_format_##type(args->hdr,dst,tag,args->tmp_arr1,nvals*nsmpl); \ } \ else \ bcf_update_format_##type(args->hdr,dst,tag,args->tmp_arr1,nvals_ori*nsmpl); \ } switch (bcf_hdr_id2type(args->hdr,BCF_HL_FMT,fmt->id)) { case BCF_HT_INT: BRANCH_NUMERIC(int32, int32_t, dst_ptr[k]=bcf_int32_missing, vals2[k]==bcf_int32_vector_end); break; case BCF_HT_REAL: BRANCH_NUMERIC(float, float, bcf_float_set_missing(dst_ptr[k]), bcf_float_is_vector_end(vals2[k])); break; } #undef BRANCH_NUMERIC } static void merge_format_string(args_t *args, bcf1_t **lines, int nlines, bcf_fmt_t *fmt, bcf1_t *dst) { const char *tag = bcf_hdr_int2id(args->hdr,BCF_DT_ID,fmt->id); int i, j, k, len = bcf_hdr_id2length(args->hdr,BCF_HL_FMT,fmt->id); if ( len!=BCF_VL_A && len!=BCF_VL_R && len!=BCF_VL_G ) { int nret = bcf_get_format_char(args->hdr,lines[0],tag,&args->tmp_arr1,&args->ntmp_arr1); bcf_update_format_char(args->hdr,dst,tag,args->tmp_arr1,nret); return; } int nsmpl = bcf_hdr_nsamples(args->hdr); for (i=0; itmp_str[i].l = 0; if ( len==BCF_VL_A || len==BCF_VL_R ) { int jfrom = len==BCF_VL_A ? 1 : 0; for (i=0; itmp_str[i]; kputc('.',tmp); for (k=jfrom+1; kn_allele; k++) kputs(",.",tmp); } for (i=0; ihdr,lines[i],tag,&args->tmp_arr1,&args->ntmp_arr1); nret /= nsmpl; for (k=0; ktmp_str[k]; char *src = (char*)args->tmp_arr1 + k*nret; for (j=jfrom; jn_allele; j++) copy_string_field(src, j-jfrom, nret, tmp, args->maps[i].map[j]-jfrom); } } } else if ( len==BCF_VL_G ) { hts_expand(uint8_t,nsmpl,args->ntmp_arr2,args->tmp_arr2); uint8_t *haploid = args->tmp_arr2; int nret = bcf_get_format_char(args->hdr,lines[0],tag,&args->tmp_arr1,&args->ntmp_arr1); nret /= nsmpl; for (i=0; itmp_arr1 + i*nret, *se = ss+nret; int nfields = 1; while ( *ss && ssn_allele ) { haploid[i] = 1; nfields = dst->n_allele; } else if ( nfields==lines[0]->n_allele*(lines[0]->n_allele+1)/2 ) { haploid[i] = 0; nfields = dst->n_allele*(dst->n_allele+1)/2; } else error("The field %s at %s:%d neither diploid nor haploid?\n", tag,bcf_seqname(args->hdr,dst),dst->pos+1); kstring_t *tmp = &args->tmp_str[i]; kputc('.',tmp); for (j=1; jhdr,lines[i],tag,&args->tmp_arr1,&args->ntmp_arr1); nret /= nsmpl; } for (k=0; ktmp_str[k]; char *src = (char*)args->tmp_arr1 + k*nret; if ( haploid[k] ) { for (j=0; jn_allele; j++) copy_string_field(src,j,nret, tmp, args->maps[i].map[j]); } else { int iori, jori, kori = 0; for (iori=0; iorin_allele; iori++) { int inew = args->maps[i].map[iori]; for (jori=0; jori<=iori; jori++) { int jnew = args->maps[i].map[jori]; int knew = bcf_alleles2gt(inew,jnew); copy_string_field(src,kori,nret,tmp,knew); kori++; } } } } } } kstring_t str; str.m = args->ntmp_arr2; str.l = 0; str.s = (char*) args->tmp_arr2; int max_len = 0; for (i=0; itmp_str[i].l ) max_len = args->tmp_str[i].l; for (i=0; itmp_str[i]; kputsn(tmp->s,tmp->l,&str); for (j=tmp->l; jntmp_arr2 = str.m; args->tmp_arr2 = (uint8_t*)str.s; bcf_update_format_char(args->hdr,dst,tag,str.s,str.l); } char **merge_alleles(char **a, int na, int *map, char **b, int *nb, int *mb); // see vcfmerge.c static void merge_biallelics_to_multiallelic(args_t *args, bcf1_t *dst, bcf1_t **lines, int nlines) { int i; for (i=0; irid = lines[0]->rid; dst->pos = lines[0]->pos; // take max for QUAL bcf_float_set_missing(dst->qual); for (i=0; iqual)) continue; if (bcf_float_is_missing(dst->qual) || dst->qualqual) dst->qual = lines[i]->qual; } bcf_update_id(args->hdr, dst, lines[0]->d.id); // Merge and set the alleles, create a mapping from source allele indexes to dst idxs hts_expand0(map_t,nlines,args->mmaps,args->maps); // a mapping for each line args->nals = args->maps[0].nals = lines[0]->n_allele; hts_expand(int,args->maps[0].nals,args->maps[0].mals,args->maps[0].map); hts_expand(char*,args->nals,args->mals,args->als); for (i=0; imaps[0].nals; i++) { args->maps[0].map[i] = i; args->als[i] = strdup(lines[0]->d.allele[i]); } for (i=1; id.id[0]!='.' || lines[i]->d.id[1]) { kstring_t tmp = {0,0,0}; if (dst->d.id[0]=='.' && !dst->d.id[1]) kputs(lines[i]->d.id, &tmp); else ksprintf(&tmp, "%s;%s", dst->d.id, lines[i]->d.id); bcf_update_id(args->hdr, dst, tmp.s); free(tmp.s); } args->maps[i].nals = lines[i]->n_allele; hts_expand(int,args->maps[i].nals,args->maps[i].mals,args->maps[i].map); args->als = merge_alleles(lines[i]->d.allele, lines[i]->n_allele, args->maps[i].map, args->als, &args->nals, &args->mals); if ( !args->als ) error("Failed to merge alleles at %s:%d\n", bcf_seqname(args->hdr,dst),dst->pos+1); } bcf_update_alleles(args->hdr, dst, (const char**)args->als, args->nals); for (i=0; inals; i++) free(args->als[i]); if ( lines[0]->d.n_flt ) bcf_update_filter(args->hdr, dst, lines[0]->d.flt, lines[0]->d.n_flt); for (i=1; id.n_flt; j++) { // if strict_filter, set FILTER to PASS if any site PASS // otherwise accumulate FILTERs if (lines[i]->d.flt[j] == bcf_hdr_id2int(args->hdr, BCF_DT_ID, "PASS")) { if (args->strict_filter) { bcf_update_filter(args->hdr, dst, lines[i]->d.flt, lines[i]->d.n_flt); break; } else continue; } bcf_add_filter(args->hdr, dst, lines[i]->d.flt[j]); } } // merge info for (i=0; in_info; i++) { bcf_info_t *info = &lines[0]->d.info[i]; int type = bcf_hdr_id2type(args->hdr,BCF_HL_INFO,info->key); if ( type==BCF_HT_INT || type==BCF_HT_REAL ) merge_info_numeric(args, lines, nlines, info, dst); else if ( type==BCF_HT_FLAG ) merge_info_flag(args, lines, nlines, info, dst); else merge_info_string(args, lines, nlines, info, dst); } // merge format int gt_id = bcf_hdr_id2int(args->hdr,BCF_DT_ID,"GT"); dst->n_sample = lines[0]->n_sample; for (i=0; in_fmt; i++) { bcf_fmt_t *fmt = &lines[0]->d.fmt[i]; int type = bcf_hdr_id2type(args->hdr,BCF_HL_FMT,fmt->id); if ( fmt->id==gt_id ) merge_format_genotype(args, lines, nlines, fmt, dst); else if ( type==BCF_HT_INT || type==BCF_HT_REAL ) merge_format_numeric(args, lines, nlines, fmt, dst); else merge_format_string(args, lines, nlines, fmt, dst); } } #define SWAP(type_t, a, b) { type_t t = a; a = b; b = t; } static void mrows_schedule(args_t *args, bcf1_t **line) { int i,m; if ( args->mrows_collapse==COLLAPSE_ANY || bcf_get_variant_types(*line)&COLLAPSE_SNPS ) { args->nalines++; m = args->malines; hts_expand(bcf1_t*,args->nalines,args->malines,args->alines); for (i=m; imalines; i++) args->alines[i] = bcf_init1(); SWAP(bcf1_t*, args->alines[args->nalines-1], *line); } else { args->nblines++; m = args->mblines; hts_expand(bcf1_t*,args->nblines,args->mblines,args->blines); for (i=m; imblines; i++) args->blines[i] = bcf_init1(); SWAP(bcf1_t*, args->blines[args->nblines-1], *line); } } static int mrows_ready_to_flush(args_t *args, bcf1_t *line) { if ( args->nalines && (args->alines[0]->rid!=line->rid || args->alines[0]->pos!=line->pos) ) return 1; if ( args->nblines && (args->blines[0]->rid!=line->rid || args->blines[0]->pos!=line->pos) ) return 1; return 0; } static bcf1_t *mrows_flush(args_t *args) { if ( args->nalines ) { if ( args->nalines==1 ) { args->nalines = 0; return args->alines[0]; } bcf_clear(args->mrow_out); merge_biallelics_to_multiallelic(args, args->mrow_out, args->alines, args->nalines); args->nalines = 0; return args->mrow_out; } else if ( args->nblines ) { if ( args->nblines==1 ) { args->nblines = 0; return args->blines[0]; } bcf_clear(args->mrow_out); merge_biallelics_to_multiallelic(args, args->mrow_out, args->blines, args->nblines); args->nblines = 0; return args->mrow_out; } return NULL; } static void flush_buffer(args_t *args, htsFile *file, int n) { bcf1_t *line; int i, k, prev_rid = -1, prev_pos = 0, prev_type = 0; for (i=0; irbuf); if ( args->mrows_op==MROWS_SPLIT ) { int split = 1; if ( args->mrows_collapse!=COLLAPSE_BOTH && args->mrows_collapse!=COLLAPSE_ANY ) { if ( !(bcf_get_variant_types(args->lines[k]) & args->mrows_collapse) ) split = 0; } if ( split && args->lines[k]->n_allele>2 ) { split_multiallelic_to_biallelics(args, args->lines[k]); int j; for (j=0; jntmp_lines; j++) bcf_write1(file, args->hdr, args->tmp_lines[j]); continue; } } if ( args->mrows_op==MROWS_MERGE ) { if ( mrows_ready_to_flush(args, args->lines[k]) ) { while ( (line=mrows_flush(args)) ) bcf_write1(file, args->hdr, line); } int merge = 1; if ( args->mrows_collapse!=COLLAPSE_BOTH && args->mrows_collapse!=COLLAPSE_ANY ) { if ( !(bcf_get_variant_types(args->lines[k]) & args->mrows_collapse) ) merge = 0; } if ( merge ) { mrows_schedule(args, &args->lines[k]); continue; } } // todo: merge with next record if POS and the type are same. For now, just discard if asked to do so. if ( args->rmdup ) { int line_type = bcf_get_variant_types(args->lines[k]); if ( prev_rid>=0 && prev_rid==args->lines[k]->rid && prev_pos==args->lines[k]->pos && prev_type==line_type ) continue; prev_rid = args->lines[k]->rid; prev_pos = args->lines[k]->pos; prev_type = line_type; } bcf_write1(file, args->hdr, args->lines[k]); } if ( args->mrows_op==MROWS_MERGE && !args->rbuf.n ) { while ( (line=mrows_flush(args)) ) bcf_write1(file, args->hdr, line); } } static void init_data(args_t *args) { args->hdr = args->files->readers[0].header; rbuf_init(&args->rbuf, 100); args->lines = (bcf1_t**) calloc(args->rbuf.m, sizeof(bcf1_t*)); if ( args->ref_fname ) { args->fai = fai_load(args->ref_fname); if ( !args->fai ) error("Failed to load the fai index: %s\n", args->ref_fname); } if ( args->mrows_op==MROWS_MERGE ) { args->mrow_out = bcf_init1(); args->tmp_str = (kstring_t*) calloc(bcf_hdr_nsamples(args->hdr),sizeof(kstring_t)); } } static void destroy_data(args_t *args) { int i; for (i=0; irbuf.m; i++) if ( args->lines[i] ) bcf_destroy1(args->lines[i]); free(args->lines); for (i=0; imtmp_lines; i++) bcf_destroy1(args->tmp_lines[i]); free(args->tmp_lines); for (i=0; inalines; i++) bcf_destroy1(args->alines[i]); free(args->alines); for (i=0; inblines; i++) bcf_destroy1(args->blines[i]); free(args->blines); for (i=0; immaps; i++) free(args->maps[i].map); for (i=0; intmp_als; i++) free(args->tmp_als[i].s); free(args->tmp_als); free(args->tmp_als_str.s); if ( args->tmp_str ) { for (i=0; ihdr); i++) free(args->tmp_str[i].s); free(args->tmp_str); } free(args->maps); free(args->als); free(args->tmp_arr1); free(args->tmp_arr2); if ( args->mrow_out ) bcf_destroy1(args->mrow_out); if ( args->fai ) fai_destroy(args->fai); if ( args->mseq ) free(args->seq); if ( args->aln.nmat ) free(args->aln.mat); if ( args->aln.m_arr ) { free(args->aln.ipos_arr); free(args->aln.lref_arr); free(args->aln.lseq_arr); } } static void normalize_vcf(args_t *args) { htsFile *out = hts_open(args->output_fname, hts_bcf_wmode(args->output_type)); if ( out == NULL ) error("Can't write to \"%s\": %s\n", args->output_fname, strerror(errno)); bcf_hdr_append_version(args->hdr, args->argc, args->argv, "bcftools_norm"); bcf_hdr_write(out, args->hdr); while ( bcf_sr_next_line(args->files) ) { args->ntotal++; bcf1_t *line = args->files->readers[0].buffer[0]; if ( args->fai ) { if ( realign(args, line)<0 && args->check_ref & CHECK_REF_SKIP ) { args->nskipped++; continue; // exclude broken VCF lines } } // still on the same chromosome? int i, j, ilast = rbuf_last(&args->rbuf); if ( ilast>=0 && line->rid != args->lines[ilast]->rid ) flush_buffer(args, out, args->rbuf.n); // new chromosome // insert into sorted buffer i = j = ilast = rbuf_append(&args->rbuf); if ( !args->lines[i] ) args->lines[i] = bcf_init1(); SWAP(bcf1_t*, args->files->readers[0].buffer[0], args->lines[i]); while ( rbuf_prev(&args->rbuf,&i) ) { if ( args->lines[i]->pos > args->lines[j]->pos ) SWAP(bcf1_t*, args->lines[i], args->lines[j]); j = i; } // find out how many sites to flush j = 0; for (i=-1; rbuf_next(&args->rbuf,&i); ) { if ( args->lines[ilast]->pos - args->lines[i]->pos < args->buf_win ) break; j++; } if ( args->rbuf.n==args->rbuf.m ) j = 1; if ( j>0 ) flush_buffer(args, out, j); } flush_buffer(args, out, args->rbuf.n); hts_close(out); fprintf(stderr,"Lines total/modified/skipped:\t%d/%d/%d\n", args->ntotal,args->nchanged,args->nskipped); } static void usage(void) { fprintf(stderr, "\n"); fprintf(stderr, "About: Left-align and normalize indels; check if REF alleles match the reference;\n"); fprintf(stderr, " split multiallelic sites into multiple rows; recover multiallelics from\n"); fprintf(stderr, " multiple rows.\n"); fprintf(stderr, "Usage: bcftools norm [options] \n"); fprintf(stderr, "\n"); fprintf(stderr, "Options:\n"); fprintf(stderr, " -c, --check-ref check REF alleles and exit (e), warn (w), exclude (x) bad sites [e]\n"); fprintf(stderr, " -D, --remove-duplicates remove duplicate lines of the same type.\n"); fprintf(stderr, " -f, --fasta-ref reference sequence\n"); fprintf(stderr, " -m, --multiallelics <-|+>[type] split multiallelics (-) or join biallelics (+), type: snps|indels|both|any [both]\n"); fprintf(stderr, " -o, --output write output to a file [standard output]\n"); fprintf(stderr, " -O, --output-type 'b' compressed BCF; 'u' uncompressed BCF; 'z' compressed VCF; 'v' uncompressed VCF [v]\n"); fprintf(stderr, " -r, --regions restrict to comma-separated list of regions\n"); fprintf(stderr, " -R, --regions-file restrict to regions listed in a file\n"); fprintf(stderr, " -s, --strict-filter when merging (-m+), merged site is PASS only if all sites being merged PASS\n"); fprintf(stderr, " -t, --targets similar to -r but streams rather than index-jumps\n"); fprintf(stderr, " -T, --targets-file similar to -R but streams rather than index-jumps\n"); fprintf(stderr, " -w, --site-win buffer for sorting lines which changed position during realignment [1000]\n"); fprintf(stderr, "\n"); exit(1); } int main_vcfnorm(int argc, char *argv[]) { int c; args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->argc = argc; args->argv = argv; args->files = bcf_sr_init(); args->output_fname = "-"; args->output_type = FT_VCF; args->aln_win = 100; args->buf_win = 1000; args->mrows_collapse = COLLAPSE_BOTH; int region_is_file = 0; int targets_is_file = 0; static struct option loptions[] = { {"help",0,0,'h'}, {"fasta-ref",1,0,'f'}, {"multiallelics",1,0,'m'}, {"regions",1,0,'r'}, {"regions-file",1,0,'R'}, {"targets",1,0,'t'}, {"targets-file",1,0,'T'}, {"site-win",1,0,'W'}, {"remove-duplicates",0,0,'D'}, {"output",1,0,'o'}, {"output-type",1,0,'O'}, {"check-ref",1,0,'c'}, {"strict-filter",0,0,'s'}, {0,0,0,0} }; char *tmp; while ((c = getopt_long(argc, argv, "hr:R:f:w:Do:O:c:m:t:T:s",loptions,NULL)) >= 0) { switch (c) { case 'm': if ( optarg[0]=='-' ) args->mrows_op = MROWS_SPLIT; else if ( optarg[0]=='+' ) args->mrows_op = MROWS_MERGE; else error("Expected '+' or '-' with -m\n"); if ( optarg[1]!=0 ) { if ( !strcmp("snps",optarg+1) ) args->mrows_collapse = COLLAPSE_SNPS; else if ( !strcmp("indels",optarg+1) ) args->mrows_collapse = COLLAPSE_INDELS; else if ( !strcmp("both",optarg+1) ) args->mrows_collapse = COLLAPSE_BOTH; else if ( !strcmp("any",optarg+1) ) args->mrows_collapse = COLLAPSE_ANY; else error("The argument to -m not recognised: %s\n", optarg); } break; case 'c': if ( strchr(optarg,'w') ) args->check_ref |= CHECK_REF_WARN; if ( strchr(optarg,'x') ) args->check_ref |= CHECK_REF_SKIP; if ( strchr(optarg,'e') ) args->check_ref = CHECK_REF_EXIT; // overrides the above break; case 'O': switch (optarg[0]) { case 'b': args->output_type = FT_BCF_GZ; break; case 'u': args->output_type = FT_BCF; break; case 'z': args->output_type = FT_VCF_GZ; break; case 'v': args->output_type = FT_VCF; break; default: error("The output type \"%s\" not recognised\n", optarg); } break; case 'o': args->output_fname = optarg; break; case 'D': args->rmdup = 1; break; case 's': args->strict_filter = 1; break; case 'f': args->ref_fname = optarg; break; case 'r': args->region = optarg; break; case 'R': args->region = optarg; region_is_file = 1; break; case 't': args->targets = optarg; break; case 'T': args->targets = optarg; targets_is_file = 1; break; case 'w': args->buf_win = strtol(optarg,&tmp,10); if ( *tmp ) error("Could not parse argument: --site-win %s\n", optarg); break; case 'h': case '?': usage(); default: error("Unknown argument: %s\n", optarg); } } if ( argc>optind+1 ) usage(); if ( !args->ref_fname && !args->mrows_op && !args->rmdup ) usage(); char *fname = NULL; if ( optind>=argc ) { if ( !isatty(fileno((FILE *)stdin)) ) fname = "-"; // reading from stdin else usage(); } else fname = argv[optind]; if ( args->region ) { if ( bcf_sr_set_regions(args->files, args->region,region_is_file)<0 ) error("Failed to read the regions: %s\n", args->region); } if ( args->targets ) { if ( bcf_sr_set_targets(args->files, args->targets,targets_is_file, 0)<0 ) error("Failed to read the targets: %s\n", args->targets); } if ( !bcf_sr_add_reader(args->files, fname) ) error("Failed to open %s: %s\n", fname,bcf_sr_strerror(args->files->errnum)); if ( args->mrows_op&MROWS_SPLIT && args->rmdup ) error("Cannot combine -D and -m-\n"); init_data(args); normalize_vcf(args); destroy_data(args); bcf_sr_destroy(args->files); free(args); return 0; } bcftools-1.2/vcfplugin.c000066400000000000000000000442541246371514100153450ustar00rootroot00000000000000/* vcfannotate.c -- Annotate and edit VCF/BCF files. Copyright (C) 2013-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include #include #include #include #include #include #include "bcftools.h" #include "vcmp.h" #include "filter.h" typedef struct _plugin_t plugin_t; /** * Plugin API: * ---------- * const char *about(void) * - short description used by 'bcftools plugin -l' * * const char *usage(void) * - longer description used by 'bcftools +name -h' * * int run(int argc, char **argv) * - if implemented, the control is immediately handed over to the plugin, * none of the init/process/destroy functions is called. Return 0 on * success or non-zero value on error. * * int init(int argc, char **argv, bcf_hdr_t *in_hdr, bcf_hdr_t *out_hdr) * - called once at startup, allows to initialize local variables. * Return 1 to suppress normal VCF/BCF header output, -1 on critical * errors, 0 otherwise. * * bcf1_t *process(bcf1_t *rec) * - called for each VCF record, return NULL for no output * * void destroy(void) * - called after all lines have been processed to clean up */ typedef void (*dl_version_f) (const char **, const char **); typedef int (*dl_run_f) (int, char **); typedef int (*dl_init_f) (int, char **, bcf_hdr_t *, bcf_hdr_t *); typedef char* (*dl_about_f) (void); typedef char* (*dl_usage_f) (void); typedef bcf1_t* (*dl_process_f) (bcf1_t *); typedef void (*dl_destroy_f) (void); struct _plugin_t { int argc; char *name, **argv; dl_version_f version; dl_run_f run; dl_init_f init; dl_about_f about; dl_usage_f usage; dl_process_f process; dl_destroy_f destroy; void *handle; }; struct _args_t; typedef struct _rm_tag_t { char *key; int hdr_id; void (*handler)(struct _args_t *, bcf1_t *, struct _rm_tag_t *); } rm_tag_t; typedef struct { char **cols; int ncols, mcols; char **als; int nals, mals; kstring_t line; int rid, start, end; } annot_line_t; typedef struct _annot_col_t { int icol, replace; char *hdr_key; int (*setter)(struct _args_t *, bcf1_t *, struct _annot_col_t *, void*); } annot_col_t; // Logic of the filters: include or exclude sites which match the filters? #define FLT_INCLUDE 1 #define FLT_EXCLUDE 2 typedef struct _args_t { bcf_srs_t *files; bcf_hdr_t *hdr, *hdr_out; htsFile *out_fh; int output_type; filter_t *filter; char *filter_str; int filter_logic; // include or exclude sites which match the filters? One of FLT_INCLUDE/FLT_EXCLUDE plugin_t plugin; int nplugin_paths; char **plugin_paths; char **argv, *output_fname, *regions_list, *targets_list; int argc, drop_header, verbose; } args_t; char *msprintf(const char *fmt, ...); static void init_plugin_paths(args_t *args) { if ( args->nplugin_paths!=-1 ) return; char *path = getenv("BCFTOOLS_PLUGINS"); if ( path ) { args->nplugin_paths = 1; args->plugin_paths = (char**) malloc(sizeof(char*)); char *ss = args->plugin_paths[0] = strdup(path); while ( *ss ) { if ( *ss==':' ) { *ss = 0; args->plugin_paths = (char**) realloc(args->plugin_paths,sizeof(char*)*(args->nplugin_paths+1)); args->plugin_paths[args->nplugin_paths] = ss+1; args->nplugin_paths++; } ss++; } } else args->nplugin_paths = 0; } static void *dlopen_plugin(args_t *args, const char *fname) { init_plugin_paths(args); void *handle; char *tmp; if ( fname[0]!='/' ) // not an absolute path { int i; for (i=0; inplugin_paths; i++) { tmp = msprintf("%s/%s.so", args->plugin_paths[i],fname); handle = dlopen(tmp, RTLD_NOW); // valgrind complains about unfreed memory, not our problem though if ( args->verbose ) { if ( !handle ) fprintf(stderr,"%s:\n\tdlopen .. %s\n", tmp,dlerror()); else fprintf(stderr,"%s:\n\tdlopen .. ok\n", tmp); } free(tmp); if ( handle ) return handle; } } handle = dlopen(fname, RTLD_NOW); if ( args->verbose ) { if ( !handle ) fprintf(stderr,"%s:\n\tdlopen .. %s\n", fname,dlerror()); else fprintf(stderr,"%s:\n\tdlopen .. ok\n", fname); } return handle; } static void print_plugin_usage_hint(void) { fprintf(stderr, "\nNo functional bcftools plugins were found"); if ( !getenv("BCFTOOLS_PLUGINS") ) fprintf(stderr,". The environment variable BCFTOOLS_PLUGINS is not set.\n\n"); else fprintf(stderr, " in\n\tBCFTOOLS_PLUGINS=\"%s\".\n\n" "- Is the plugin path correct?\n\n" "- Are all shared libraries, namely libhts.so, accessible? Verify with\n" " on Mac OS X: `otool -L your/plugin.so` and set DYLD_LIBRARY_PATH if they are not\n" " on Linux: `ldd your/plugin.so` and set LD_LIBRARY_PATH if they are not\n" "\n" "- If not installed systemwide, set the environment variable LD_LIBRARY_PATH (linux) or\n" "DYLD_LIBRARY_PATH (mac) to include directory where *libhts.so* is located.\n" "\n" "- Run \"bcftools plugin -lv\" for more detailed error output.\n" "\n", getenv("BCFTOOLS_PLUGINS") ); } static int load_plugin(args_t *args, const char *fname, int exit_on_error, plugin_t *plugin) { plugin->name = strdup(fname); plugin->handle = dlopen_plugin(args, fname); if ( !plugin->handle ) { if ( exit_on_error ) { print_plugin_usage_hint(); error("Could not load \"%s\".\n\n", fname); } return -1; } dlerror(); plugin->init = (dl_init_f) dlsym(plugin->handle, "init"); char *ret = dlerror(); if ( ret ) plugin->init = NULL; else if ( args->verbose ) fprintf(stderr,"\tinit .. ok\n"); plugin->run = (dl_run_f) dlsym(plugin->handle, "run"); ret = dlerror(); if ( ret ) plugin->run = NULL; else if ( args->verbose ) fprintf(stderr,"\trun .. ok\n"); if ( !plugin->init && !plugin->run ) { if ( exit_on_error ) error("Could not initialize %s, neither run or init found \n", plugin->name); else if ( args->verbose ) fprintf(stderr,"\tinit/run .. not found\n"); return -1; } plugin->version = (dl_version_f) dlsym(plugin->handle, "version"); ret = dlerror(); if ( ret ) { if ( exit_on_error ) error("Could not initialize %s, version string not found\n", plugin->name); else if ( args->verbose ) fprintf(stderr,"\tversion .. not found\n"); return -1; } plugin->about = (dl_about_f) dlsym(plugin->handle, "about"); ret = dlerror(); if ( ret ) { if ( exit_on_error ) error("Could not initialize %s: %s\n", plugin->name, ret); return -1; } plugin->usage = (dl_about_f) dlsym(plugin->handle, "usage"); ret = dlerror(); if ( ret ) plugin->usage = plugin->about; if ( plugin->run ) return 0; plugin->process = (dl_process_f) dlsym(plugin->handle, "process"); ret = dlerror(); if ( ret ) { if ( exit_on_error ) error("Could not initialize %s: %s\n", plugin->name, ret); return -1; } plugin->destroy = (dl_destroy_f) dlsym(plugin->handle, "destroy"); ret = dlerror(); if ( ret ) { if ( exit_on_error ) error("Could not initialize %s: %s\n", plugin->name, ret); return -1; } return 0; } static void init_plugin(args_t *args) { static int warned_bcftools = 0, warned_htslib = 0; int ret = args->plugin.init(args->plugin.argc,args->plugin.argv,args->hdr,args->hdr_out); if ( ret<0 ) error("The plugin exited with an error.\n"); const char *bver, *hver; args->plugin.version(&bver, &hver); if ( strcmp(bver,bcftools_version()) && !warned_bcftools ) { fprintf(stderr,"WARNING: bcftools version mismatch .. bcftools at %s, the plugin \"%s\" at %s\n", bcftools_version(),args->plugin.name,bver); warned_bcftools = 1; } if ( strcmp(hver,hts_version()) && !warned_htslib ) { fprintf(stderr,"WARNING: htslib version mismatch .. bcftools at %s, the plugin \"%s\" at %s\n", hts_version(),args->plugin.name,hver); warned_htslib = 1; } args->drop_header += ret; } static int cmp_plugin_name(const void *p1, const void *p2) { plugin_t *a = (plugin_t*) p1; plugin_t *b = (plugin_t*) p2; return strcmp(a->name,b->name); } static int list_plugins(args_t *args) { plugin_t *plugins = NULL; int nplugins = 0, mplugins = 0; init_plugin_paths(args); kstring_t str = {0,0,0}; int i; for (i=0; inplugin_paths; i++) { DIR *dp = opendir(args->plugin_paths[i]); if ( dp==NULL ) continue; struct dirent *ep; while ( (ep=readdir(dp)) ) { int len = strlen(ep->d_name); if ( strcasecmp(".so",ep->d_name+len-3) ) continue; str.l = 0; ksprintf(&str,"%s/%s", args->plugin_paths[i],ep->d_name); hts_expand(plugin_t, nplugins+1, mplugins, plugins); if ( load_plugin(args, str.s, 0, &plugins[nplugins]) < 0 ) continue; nplugins++; str.l = 0; kputs(ep->d_name, &str); int l = str.l - 1; while ( l>=0 && str.s[l]!='.' ) l--; if ( l>=0 ) str.s[l] = 0; free(plugins[nplugins-1].name); plugins[nplugins-1].name = strdup(str.s); // use a short name } closedir(dp); } if ( nplugins ) { qsort(plugins, nplugins, sizeof(plugins[0]), cmp_plugin_name); for (i=0; ihdr = args->files->readers[0].header; args->hdr_out = bcf_hdr_dup(args->hdr); init_plugin(args); if ( args->filter_str ) args->filter = filter_init(args->hdr, args->filter_str); bcf_hdr_append_version(args->hdr_out, args->argc, args->argv, "bcftools_plugin"); if ( !args->drop_header ) { args->out_fh = hts_open(args->output_fname,hts_bcf_wmode(args->output_type)); if ( args->out_fh == NULL ) error("Can't write to \"%s\": %s\n", args->output_fname, strerror(errno)); bcf_hdr_write(args->out_fh, args->hdr_out); } } static void destroy_data(args_t *args) { free(args->plugin.name); if ( args->plugin.destroy ) args->plugin.destroy(); dlclose(args->plugin.handle); if ( args->hdr_out ) bcf_hdr_destroy(args->hdr_out); if ( args->nplugin_paths>0 ) { free(args->plugin_paths[0]); free(args->plugin_paths); } if ( args->filter ) filter_destroy(args->filter); if (args->out_fh) hts_close(args->out_fh); } static void usage(args_t *args) { fprintf(stderr, "\n"); fprintf(stderr, "About: Run user defined plugin\n"); fprintf(stderr, "Usage: bcftools plugin [OPTIONS] [-- PLUGIN_OPTIONS]\n"); fprintf(stderr, " bcftools +name [OPTIONS] [-- PLUGIN_OPTIONS]\n"); fprintf(stderr, "\n"); fprintf(stderr, "VCF input options:\n"); fprintf(stderr, " -e, --exclude exclude sites for which the expression is true\n"); fprintf(stderr, " -i, --include select sites for which the expression is true\n"); fprintf(stderr, " -r, --regions restrict to comma-separated list of regions\n"); fprintf(stderr, " -R, --regions-file restrict to regions listed in a file\n"); fprintf(stderr, " -t, --targets similar to -r but streams rather than index-jumps\n"); fprintf(stderr, " -T, --targets-file similar to -R but streams rather than index-jumps\n"); fprintf(stderr, "VCF output options:\n"); fprintf(stderr, " -o, --output write output to a file [standard output]\n"); fprintf(stderr, " -O, --output-type 'b' compressed BCF; 'u' uncompressed BCF; 'z' compressed VCF; 'v' uncompressed VCF [v]\n"); fprintf(stderr, "Plugin options:\n"); fprintf(stderr, " -h, --help list plugin's options\n"); fprintf(stderr, " -l, --list-plugins list available plugins. See BCFTOOLS_PLUGINS environment variable and man page for details\n"); fprintf(stderr, " -v, --verbose print debugging information on plugin failure\n"); fprintf(stderr, "\n"); exit(1); } int main_plugin(int argc, char *argv[]) { int c; args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->argc = argc; args->argv = argv; args->output_fname = "-"; args->output_type = FT_VCF; args->nplugin_paths = -1; int regions_is_file = 0, targets_is_file = 0, plist_only = 0; if ( argc==1 ) usage(args); char *plugin_name = NULL; if ( argv[1][0]!='-' ) { plugin_name = argv[1]; argc--; argv++; } static struct option loptions[] = { {"verbose",0,0,'v'}, {"help",0,0,'h'}, {"list-plugins",0,0,'l'}, {"output",1,0,'o'}, {"output-type",1,0,'O'}, {"include",1,0,'i'}, {"exclude",1,0,'e'}, {"regions",1,0,'r'}, {"regions-file",1,0,'R'}, {"targets",1,0,'t'}, {"targets-file",1,0,'T'}, {0,0,0,0} }; while ((c = getopt_long(argc, argv, "h?o:O:r:R:li:e:v",loptions,NULL)) >= 0) { switch (c) { case 'v': args->verbose = 1; break; case 'o': args->output_fname = optarg; break; case 'O': switch (optarg[0]) { case 'b': args->output_type = FT_BCF_GZ; break; case 'u': args->output_type = FT_BCF; break; case 'z': args->output_type = FT_VCF_GZ; break; case 'v': args->output_type = FT_VCF; break; default: error("The output type \"%s\" not recognised\n", optarg); }; break; case 'e': args->filter_str = optarg; args->filter_logic |= FLT_EXCLUDE; break; case 'i': args->filter_str = optarg; args->filter_logic |= FLT_INCLUDE; break; case 'r': args->regions_list = optarg; break; case 'R': args->regions_list = optarg; regions_is_file = 1; break; case 't': args->targets_list = optarg; break; case 'T': args->targets_list = optarg; targets_is_file = 1; break; case 'l': plist_only = 1; break; case '?': case 'h': load_plugin(args, plugin_name, 1, &args->plugin); fprintf(stderr,"%s",args->plugin.usage()); return 0; break; default: error("Unknown argument: %s\n", optarg); } } if ( plist_only ) return list_plugins(args); load_plugin(args, plugin_name, 1, &args->plugin); if ( args->plugin.run ) { int iopt = optind; optind = 0; int ret = args->plugin.run(argc-iopt, argv+iopt); destroy_data(args); free(args); return ret; } char *fname = NULL; if ( optind>=argc || argv[optind][0]=='-' ) { if ( !isatty(fileno((FILE *)stdin)) ) fname = "-"; // reading from stdin else usage(args); args->plugin.argc = argc - optind + 1; args->plugin.argv = argv + optind - 1; } else { fname = argv[optind]; args->plugin.argc = argc - optind; args->plugin.argv = argv + optind; } optind = 0; args->plugin.argv[0] = plugin_name; args->files = bcf_sr_init(); if ( args->regions_list ) { if ( bcf_sr_set_regions(args->files, args->regions_list, regions_is_file)<0 ) error("Failed to read the regions: %s\n", args->regions_list); } if ( args->targets_list ) { if ( bcf_sr_set_targets(args->files, args->targets_list, targets_is_file, 0)<0 ) error("Failed to read the targets: %s\n", args->targets_list); args->files->collapse |= COLLAPSE_SOME; } if ( !bcf_sr_add_reader(args->files, fname) ) error("Failed to open %s: %s\n", fname,bcf_sr_strerror(args->files->errnum)); init_data(args); while ( bcf_sr_next_line(args->files) ) { bcf1_t *line = bcf_sr_get_line(args->files,0); if ( args->filter ) { int pass = filter_test(args->filter, line, NULL); if ( args->filter_logic & FLT_EXCLUDE ) pass = pass ? 0 : 1; if ( !pass ) continue; } line = args->plugin.process(line); if ( line ) bcf_write1(args->out_fh, args->hdr_out, line); } destroy_data(args); bcf_sr_destroy(args->files); free(args); return 0; } bcftools-1.2/vcfquery.c000066400000000000000000000336051246371514100152120ustar00rootroot00000000000000/* vcfquery.c -- Extracts fields from VCF/BCF file. Copyright (C) 2013-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include #include #include "bcftools.h" #include "filter.h" #include "convert.h" // Logic of the filters: include or exclude sites which match the filters? #define FLT_INCLUDE 1 #define FLT_EXCLUDE 2 typedef struct { filter_t *filter; char *filter_str; int filter_logic; // include or exclude sites which match the filters? One of FLT_INCLUDE/FLT_EXCLUDE convert_t *convert; bcf_srs_t *files; bcf_hdr_t *header; int nsamples, *samples, sample_is_file; char **argv, *format_str, *sample_list, *targets_list, *regions_list, *vcf_list, *fn_out; int argc, list_columns, print_header; FILE *out; } args_t; static void destroy_list(char **list, int n) { int i; for (i=0; iheader = args->files->readers[0].header; int i, nsamples = 0, *samples = NULL; if ( args->sample_list && strcmp("-",args->sample_list) ) { for (i=0; ifiles->nreaders; i++) { int ret = bcf_hdr_set_samples(args->files->readers[i].header,args->sample_list,args->sample_is_file); if ( ret<0 ) error("Error parsing the sample list\n"); else if ( ret>0 ) error("Sample name mismatch: sample #%d not found in the header\n", ret); } if ( args->sample_list[0]!='^' ) { // the sample ordering may be different if not negated int n; char **smpls = hts_readlist(args->sample_list, args->sample_is_file, &n); if ( !smpls ) error("Could not parse %s\n", args->sample_list); if ( n!=bcf_hdr_nsamples(args->files->readers[0].header) ) error("The number of samples does not match, perhaps some are present multiple times?\n"); nsamples = bcf_hdr_nsamples(args->files->readers[0].header); samples = (int*) malloc(sizeof(int)*nsamples); for (i=0; ifiles->readers[0].header, BCF_DT_SAMPLE,smpls[i]); free(smpls[i]); } free(smpls); } } args->convert = convert_init(args->header, samples, nsamples, args->format_str); free(samples); if ( args->filter_str ) args->filter = filter_init(args->header, args->filter_str); } static void destroy_data(args_t *args) { convert_destroy(args->convert); if ( args->filter ) filter_destroy(args->filter); free(args->samples); } static void query_vcf(args_t *args) { kstring_t str = {0,0,0}; if ( args->print_header ) { convert_header(args->convert,&str); fwrite(str.s, str.l, 1, args->out); } while ( bcf_sr_next_line(args->files) ) { if ( !bcf_sr_has_line(args->files,0) ) continue; bcf1_t *line = args->files->readers[0].buffer[0]; bcf_unpack(line, args->files->max_unpack); if ( args->filter ) { int pass = filter_test(args->filter, line, NULL); if ( args->filter_logic & FLT_EXCLUDE ) pass = pass ? 0 : 1; if ( !pass ) continue; } str.l = 0; convert_line(args->convert, line, &str); if ( str.l ) fwrite(str.s, str.l, 1, args->out); } if ( str.m ) free(str.s); } static void list_columns(args_t *args) { int i; bcf_sr_t *reader = &args->files->readers[0]; for (i=0; iheader); i++) printf("%s\n", reader->header->samples[i]); } static char **copy_header(bcf_hdr_t *hdr, char **src, int nsrc) { char **dst = (char**) malloc(sizeof(char*)*nsrc); int i; for (i=0; i [ [...]]\n"); fprintf(stderr, "\n"); fprintf(stderr, "Options:\n"); fprintf(stderr, " -c, --collapse collapse lines with duplicate positions for , see man page [none]\n"); fprintf(stderr, " -e, --exclude exclude sites for which the expression is true (see man page for details)\n"); fprintf(stderr, " -f, --format see man page for details\n"); fprintf(stderr, " -H, --print-header print header\n"); fprintf(stderr, " -i, --include select sites for which the expression is true (see man page for details)\n"); fprintf(stderr, " -l, --list-samples print the list of samples and exit\n"); fprintf(stderr, " -o, --output-file output file name [stdout]\n"); fprintf(stderr, " -r, --regions restrict to comma-separated list of regions\n"); fprintf(stderr, " -R, --regions-file restrict to regions listed in a file\n"); fprintf(stderr, " -s, --samples list of samples to include\n"); fprintf(stderr, " -S, --samples-file file of samples to include\n"); fprintf(stderr, " -t, --targets similar to -r but streams rather than index-jumps\n"); fprintf(stderr, " -T, --targets-file similar to -R but streams rather than index-jumps\n"); fprintf(stderr, " -v, --vcf-list process multiple VCFs listed in the file\n"); fprintf(stderr, "\n"); fprintf(stderr, "Examples:\n"); fprintf(stderr, "\tbcftools query -f '%%CHROM\\t%%POS\\t%%REF\\t%%ALT[\\t%%SAMPLE=%%GT]\\n' file.vcf.gz\n"); fprintf(stderr, "\n"); exit(1); } int main_vcfquery(int argc, char *argv[]) { int c, collapse = 0; args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->argc = argc; args->argv = argv; int regions_is_file = 0, targets_is_file = 0; static struct option loptions[] = { {"help",0,0,'h'}, {"list-samples",0,0,'l'}, {"include",1,0,'i'}, {"exclude",1,0,'e'}, {"format",1,0,'f'}, {"output-file",1,0,'o'}, {"regions",1,0,'r'}, {"regions-file",1,0,'R'}, {"targets",1,0,'t'}, {"targets-file",1,0,'T'}, {"annots",1,0,'a'}, {"samples",1,0,'s'}, {"samples-file",1,0,'S'}, {"print-header",0,0,'H'}, {"collapse",1,0,'c'}, {"vcf-list",1,0,'v'}, {0,0,0,0} }; while ((c = getopt_long(argc, argv, "hlr:R:f:a:s:S:Ht:T:c:v:i:e:o:",loptions,NULL)) >= 0) { switch (c) { case 'o': args->fn_out = optarg; break; case 'f': args->format_str = strdup(optarg); break; case 'H': args->print_header = 1; break; case 'v': args->vcf_list = optarg; break; case 'c': if ( !strcmp(optarg,"snps") ) collapse |= COLLAPSE_SNPS; else if ( !strcmp(optarg,"indels") ) collapse |= COLLAPSE_INDELS; else if ( !strcmp(optarg,"both") ) collapse |= COLLAPSE_SNPS | COLLAPSE_INDELS; else if ( !strcmp(optarg,"any") ) collapse |= COLLAPSE_ANY; else if ( !strcmp(optarg,"all") ) collapse |= COLLAPSE_ANY; else if ( !strcmp(optarg,"some") ) args->files->collapse |= COLLAPSE_SOME; else error("The --collapse string \"%s\" not recognised.\n", optarg); break; case 'a': { kstring_t str = {0,0,0}; kputs("%CHROM\t%POS\t%MASK\t%REF\t%ALT\t%", &str); char *p = optarg; while ( *p ) { if ( *p==',' ) kputs("\t%", &str); else kputc(*p, &str); p++; } kputc('\n', &str); args->format_str = str.s; break; } case 'e': args->filter_str = optarg; args->filter_logic |= FLT_EXCLUDE; break; case 'i': args->filter_str = optarg; args->filter_logic |= FLT_INCLUDE; break; case 'r': args->regions_list = optarg; break; case 'R': args->regions_list = optarg; regions_is_file = 1; break; case 't': args->targets_list = optarg; break; case 'T': args->targets_list = optarg; targets_is_file = 1; break; case 'l': args->list_columns = 1; break; case 's': args->sample_list = optarg; break; case 'S': args->sample_list = optarg; args->sample_is_file = 1; break; case 'h': case '?': usage(); default: error("Unknown argument: %s\n", optarg); } } char *fname = NULL; if ( optind>=argc ) { if ( !isatty(fileno((FILE *)stdin)) ) fname = "-"; } else fname = argv[optind]; if ( args->list_columns ) { if ( !fname ) error("Missing the VCF file name\n"); args->files = bcf_sr_init(); if ( !bcf_sr_add_reader(args->files, fname) ) error("Failed to open %s: %s\n", fname,bcf_sr_strerror(args->files->errnum)); list_columns(args); bcf_sr_destroy(args->files); free(args); return 0; } if ( !args->format_str ) usage(); args->out = args->fn_out ? fopen(args->fn_out, "w") : stdout; if ( !args->out ) error("%s: %s\n", args->fn_out,strerror(errno)); if ( !args->vcf_list ) { if ( !fname ) usage(); args->files = bcf_sr_init(); args->files->collapse = collapse; if ( optind+1 < argc ) args->files->require_index = 1; if ( args->regions_list && bcf_sr_set_regions(args->files, args->regions_list, regions_is_file)<0 ) error("Failed to read the regions: %s\n", args->regions_list); if ( args->targets_list ) { if ( bcf_sr_set_targets(args->files, args->targets_list, targets_is_file, 0)<0 ) error("Failed to read the targets: %s\n", args->targets_list); } while ( fname ) { if ( !bcf_sr_add_reader(args->files, fname) ) error("Failed to open %s: %s\n", fname,bcf_sr_strerror(args->files->errnum)); fname = ++optind < argc ? argv[optind] : NULL; } init_data(args); query_vcf(args); free(args->format_str); destroy_data(args); bcf_sr_destroy(args->files); fclose(args->out); free(args); return 0; } // multiple VCFs int i, k, nfiles, prev_nsamples = 0; char **fnames, **prev_samples = NULL; fnames = hts_readlist(args->vcf_list, 1, &nfiles); if ( !nfiles ) error("No files in %s?\n", args->vcf_list); for (i=0; ifiles = bcf_sr_init(); args->files->collapse = collapse; if ( args->regions_list && bcf_sr_set_regions(args->files, args->regions_list, regions_is_file)<0 ) error("Failed to read the regions: %s\n", args->regions_list); if ( optind < argc ) args->files->require_index = 1; if ( args->targets_list ) { if ( bcf_sr_set_targets(args->files, args->targets_list,targets_is_file, 0)<0 ) error("Failed to read the targets: %s\n", args->targets_list); } if ( !bcf_sr_add_reader(args->files, fnames[i]) ) error("Failed to open %s: %s\n", fnames[i],bcf_sr_strerror(args->files->errnum)); for (k=optind; kfiles, argv[k]) ) error("Failed to open %s: %s\n", argv[k],bcf_sr_strerror(args->files->errnum)); init_data(args); if ( i==0 ) prev_samples = copy_header(args->header, args->files->readers[0].header->samples, bcf_hdr_nsamples(args->files->readers[0].header)); else { args->print_header = 0; if ( compare_header(args->header, args->files->readers[0].header->samples, bcf_hdr_nsamples(args->files->readers[0].header), prev_samples, prev_nsamples) ) error("Different samples in %s and %s\n", fnames[i-1],fnames[i]); } query_vcf(args); destroy_data(args); bcf_sr_destroy(args->files); } fclose(args->out); destroy_list(fnames, nfiles); destroy_list(prev_samples, prev_nsamples); free(args->format_str); free(args); return 0; } bcftools-1.2/vcfroh.c000066400000000000000000000645011246371514100146340ustar00rootroot00000000000000/* vcfroh.c -- HMM model for detecting runs of autozygosity. Copyright (C) 2013-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include "bcftools.h" #include "HMM.h" #define STATE_HW 0 // normal state, follows Hardy-Weinberg allele frequencies #define STATE_AZ 1 // autozygous state /** Genetic map */ typedef struct { int pos; double rate; } genmap_t; typedef struct _args_t { bcf_srs_t *files; bcf_hdr_t *hdr; double t2AZ, t2HW; // P(AZ|HW) and P(HW|AZ) parameters double unseen_PL; char *genmap_fname; genmap_t *genmap; int ngenmap, mgenmap, igenmap; double rec_rate; // constant recombination rate if > 0 hmm_t *hmm; double *eprob; // emission probs [2*nsites,msites] uint32_t *sites; // positions [nsites,msites] int nsites, msites; int nrids, *rids, *rid_offs; // multiple chroms with vi_training int32_t *itmp; int nitmp, mitmp; float *AFs; int mAFs; double pl2p[256], *pdg; int32_t skip_rid, prev_rid, prev_pos; int ntot, nused; // some stats to detect if things didn't go awfully wrong int ismpl, nsmpl; // index of query sample char *estimate_AF, *sample; // list of samples for AF estimate and query sample char **argv, *targets_list, *regions_list, *af_fname, *af_tag; int argc, fake_PLs, snps_only, vi_training; } args_t; void set_tprob_genmap(hmm_t *hmm, uint32_t prev_pos, uint32_t pos, void *data); void set_tprob_recrate(hmm_t *hmm, uint32_t prev_pos, uint32_t pos, void *data); void *smalloc(size_t size) { void *mem = malloc(size); if ( !mem ) error("malloc: Could not allocate %d bytes\n", (int)size); return mem; } static void init_data(args_t *args) { args->prev_rid = args->skip_rid = -1; args->hdr = args->files->readers[0].header; if ( !args->sample ) { if ( bcf_hdr_nsamples(args->hdr)>1 ) error("Missing the option -s, --sample\n"); args->sample = strdup(args->hdr->samples[0]); } if ( !bcf_hdr_nsamples(args->hdr) ) error("No samples in the VCF?\n"); // Set samples kstring_t str = {0,0,0}; if ( args->estimate_AF && strcmp("-",args->estimate_AF) ) { int i, n; char **smpls = hts_readlist(args->estimate_AF, 1, &n); // Make sure the query sample is included for (i=0; isample,smpls[i]) ) break; // Add the query sample if not present if ( i!=n ) kputs(args->sample, &str); for (i=0; isample, &str); int ret = bcf_hdr_set_samples(args->hdr, str.s, 0); if ( ret<0 ) error("Error parsing the list of samples: %s\n", str.s); else if ( ret>0 ) error("The %d-th sample not found in the VCF\n", ret); if ( args->af_tag ) if ( !bcf_hdr_idinfo_exists(args->hdr,BCF_HL_INFO,bcf_hdr_id2int(args->hdr,BCF_DT_ID,args->af_tag)) ) error("No such INFO tag in the VCF: %s\n", args->af_tag); if ( !bcf_sr_set_samples(args->files, str.s, 0) ) error("Error: could not set the samples %s\n", str.s); args->nsmpl = args->files->n_smpl; args->ismpl = bcf_hdr_id2int(args->hdr, BCF_DT_SAMPLE, args->sample); free(str.s); int i; for (i=0; i<256; i++) args->pl2p[i] = pow(10., -i/10.); // Init transition matrix and HMM double tprob[4]; MAT(tprob,2,STATE_HW,STATE_HW) = 1 - args->t2AZ; MAT(tprob,2,STATE_HW,STATE_AZ) = args->t2HW; MAT(tprob,2,STATE_AZ,STATE_HW) = args->t2AZ; MAT(tprob,2,STATE_AZ,STATE_AZ) = 1 - args->t2HW; if ( args->genmap_fname ) { args->hmm = hmm_init(2, tprob, 0); hmm_set_tprob_func(args->hmm, set_tprob_genmap, args); } else if ( args->rec_rate > 0 ) { args->hmm = hmm_init(2, tprob, 0); hmm_set_tprob_func(args->hmm, set_tprob_recrate, args); } else args->hmm = hmm_init(2, tprob, 10000); // print header printf("# This file was produced by: bcftools roh(%s+htslib-%s)\n", bcftools_version(),hts_version()); printf("# The command line was:\tbcftools %s", args->argv[0]); for (i=1; iargc; i++) printf(" %s",args->argv[i]); printf("\n#\n"); printf("# [1]Chromosome\t[2]Position\t[3]State (0:HW, 1:AZ)\t[4]Quality\n"); } static void destroy_data(args_t *args) { free(args->sites); free(args->eprob); free(args->sample); free(args->rids); free(args->rid_offs); hmm_destroy(args->hmm); bcf_sr_destroy(args->files); free(args->itmp); free(args->AFs); free(args->pdg); free(args->genmap); } static int load_genmap(args_t *args, bcf1_t *line) { if ( !args->genmap_fname ) { args->ngenmap = 0; return 0; } kstring_t str = {0,0,0}; char *fname = strstr(args->genmap_fname,"{CHROM}"); if ( fname ) { kputsn(args->genmap_fname, fname - args->genmap_fname, &str); kputs(bcf_seqname(args->hdr,line), &str); kputs(fname+7,&str); fname = str.s; } else fname = args->genmap_fname; htsFile *fp = hts_open(fname, "rb"); if ( !fp ) { args->ngenmap = 0; return -1; } hts_getline(fp, KS_SEP_LINE, &str); if ( strcmp(str.s,"position COMBINED_rate(cM/Mb) Genetic_Map(cM)") ) error("Unexpected header, found:\n\t[%s], but expected:\n\t[position COMBINED_rate(cM/Mb) Genetic_Map(cM)]\n", fname, str.s); args->ngenmap = args->igenmap = 0; while ( hts_getline(fp, KS_SEP_LINE, &str) > 0 ) { args->ngenmap++; hts_expand(genmap_t,args->ngenmap,args->mgenmap,args->genmap); genmap_t *gm = &args->genmap[args->ngenmap-1]; char *tmp, *end; gm->pos = strtol(str.s, &tmp, 10); if ( str.s==tmp ) error("Could not parse %s: %s\n", fname, str.s); // skip second column tmp++; while ( *tmp && !isspace(*tmp) ) tmp++; // read the genetic map in cM gm->rate = strtod(tmp+1, &end); if ( tmp+1==end ) error("Could not parse %s: %s\n", fname, str.s); } if ( !args->ngenmap ) error("Genetic map empty?\n"); int i; for (i=0; ingenmap; i++) args->genmap[i].rate /= args->genmap[args->ngenmap-1].rate; // scale to 1 if ( hts_close(fp) ) error("Close failed\n"); free(str.s); return 0; } static double get_genmap_rate(args_t *args, int start, int end) { // position i to be equal to or smaller than start int i = args->igenmap; if ( args->genmap[i].pos > start ) { while ( i>0 && args->genmap[i].pos > start ) i--; } else { while ( i+1ngenmap && args->genmap[i+1].pos < start ) i++; } // position j to be equal or larger than end int j = i; while ( j+1ngenmap && args->genmap[j].pos < end ) j++; if ( i==j ) { args->igenmap = i; return 0; } if ( start < args->genmap[i].pos ) start = args->genmap[i].pos; if ( end > args->genmap[j].pos ) end = args->genmap[j].pos; double rate = (args->genmap[j].rate - args->genmap[i].rate)/(args->genmap[j].pos - args->genmap[i].pos) * (end-start); args->igenmap = j; return rate; } void set_tprob_genmap(hmm_t *hmm, uint32_t prev_pos, uint32_t pos, void *data) { args_t *args = (args_t*) data; double ci = get_genmap_rate(args, pos - prev_pos, pos); MAT(hmm->curr_tprob,2,STATE_HW,STATE_HW) *= 1-ci; MAT(hmm->curr_tprob,2,STATE_HW,STATE_AZ) *= ci; MAT(hmm->curr_tprob,2,STATE_AZ,STATE_HW) *= ci; MAT(hmm->curr_tprob,2,STATE_AZ,STATE_AZ) *= 1-ci; } void set_tprob_recrate(hmm_t *hmm, uint32_t prev_pos, uint32_t pos, void *data) { args_t *args = (args_t*) data; double ci = (pos - prev_pos) * args->rec_rate; MAT(hmm->curr_tprob,2,STATE_HW,STATE_HW) *= 1-ci; MAT(hmm->curr_tprob,2,STATE_HW,STATE_AZ) *= ci; MAT(hmm->curr_tprob,2,STATE_AZ,STATE_HW) *= ci; MAT(hmm->curr_tprob,2,STATE_AZ,STATE_AZ) *= 1-ci; } /** * This function implements the HMM model: * D = Data, AZ = autozygosity, HW = Hardy-Weinberg (non-autozygosity), * f = non-ref allele frequency * * Emission probabilities: * oAZ = P_i(D|AZ) = (1-f)*P(D|RR) + f*P(D|AA) * oHW = P_i(D|HW) = (1-f)^2 * P(D|RR) + f^2 * P(D|AA) + 2*f*(1-f)*P(D|RA) * * Transition probabilities: * tAZ = P(AZ|HW) .. parameter * tHW = P(HW|AZ) .. parameter * P(AZ|AZ) = 1 - P(HW|AZ) = 1 - tHW * P(HW|HW) = 1 - P(AZ|HW) = 1 - tAZ * * ci = P_i(C) .. probability of cross-over at site i, from genetic map * * AZi = P_i(AZ) .. probability of site i being AZ/non-AZ, scaled so that AZi+HWi = 1 * HWi = P_i(HW) * * P_i(AZ|AZ) = P(AZ|AZ) * (1-ci) * AZ{i-1} = (1-tHW) * (1-ci) * AZ{i-1} * P_i(AZ|HW) = P(AZ|HW) * ci * HW{i-1} = tAZ * ci * (1 - AZ{i-1}) * * P_i(HW|HW) = P(HW|HW) * (1-ci) * HW{i-1} = (1-tAZ) * (1-ci) * (1 - AZ{i-1}) * P_i(HW|AZ) = P(HW|AZ) * ci * AZ{i-1} = tHW * ci * AZ{i-1} */ static void flush_viterbi(args_t *args) { int i,j; if ( !args->nsites ) return; if ( !args->vi_training ) { // single viterbi pass, one chromsome hmm_run_viterbi(args->hmm, args->nsites, args->eprob, args->sites); const char *chr = bcf_hdr_id2name(args->hdr,args->prev_rid); for (i=0; insites; i++) { printf("%s\t%d\t%d\t..\n", chr,args->sites[i]+1,args->hmm->vpath[i*2]==STATE_AZ ? 1 : 0); } return; } // viterbi training, multiple chromosomes double t2az_prev, t2hw_prev; double deltaz, delthw; int niter = 0; do { t2az_prev = MAT(args->hmm->tprob_arr,2,1,0); //args->t2AZ; t2hw_prev = MAT(args->hmm->tprob_arr,2,0,1); //args->t2HW; double tcounts[] = { 0,0,0,0 }; for (i=0; inrids; i++) { // run viterbi for each chromosomes. eprob and sites contain // multiple chromosomes, rid_offs mark the boundaries int ioff = args->rid_offs[i]; int nsites = (i+1==args->nrids ? args->nsites : args->rid_offs[i+1]) - ioff; hmm_run_viterbi(args->hmm, nsites, args->eprob+ioff*2, args->sites+ioff); // what transitions were observed: add to the total counts for (j=1; jhmm->vpath[2*(j-1)]; int curr_state = args->hmm->vpath[2*j]; MAT(tcounts,2,curr_state,prev_state) += 1; } } // update the transition matrix tprob for (i=0; i<2; i++) { int n = 0; for (j=0; j<2; j++) n += MAT(tcounts,2,i,j); error("fixme: state %d not observed\n", i+1); for (j=0; j<2; j++) MAT(tcounts,2,i,j) /= n; } if ( args->genmap_fname || args->rec_rate > 0 ) hmm_set_tprob(args->hmm, tcounts, 0); else hmm_set_tprob(args->hmm, tcounts, 10000); deltaz = fabs(MAT(args->hmm->tprob_arr,2,1,0)-t2az_prev); delthw = fabs(MAT(args->hmm->tprob_arr,2,0,1)-t2hw_prev); niter++; fprintf(stderr,"%d: %f %f\n", niter,deltaz,delthw); } while ( deltaz > 0.0 || delthw > 0.0 ); fprintf(stderr, "Viterbi training converged in %d iterations to", niter); for (i=0; i<2; i++) for (j=0; j<2; j++) fprintf(stderr, " %f", MAT(args->hmm->tprob_arr,2,i,j)); fprintf(stderr, "\n"); // output the results for (i=0; inrids; i++) { int ioff = args->rid_offs[i]; int nsites = (i+1==args->nrids ? args->nsites : args->rid_offs[i+1]) - ioff; hmm_run_viterbi(args->hmm, nsites, args->eprob+ioff*2, args->sites+ioff); const char *chr = bcf_hdr_id2name(args->hdr,args->rids[i]); for (j=0; jsites[ioff+j]+1,args->hmm->vpath[j*2]==STATE_AZ ? 1 : 0); } } } static void push_rid(args_t *args, int rid) { args->nrids++; args->rids = (int*) realloc(args->rids, args->nrids*sizeof(int)); args->rid_offs = (int*) realloc(args->rid_offs, args->nrids*sizeof(int)); args->rids[ args->nrids-1 ] = rid; args->rid_offs[ args->nrids-1 ] = args->nsites; } int read_AF(bcf_sr_regions_t *tgt, bcf1_t *line, double *alt_freq) { if ( tgt->nals != line->n_allele ) return -1; // number of alleles does not match int i; for (i=0; inals; i++) if ( strcmp(line->d.allele[i],tgt->als[i]) ) break; // we could be smarter, see vcmp if ( inals ) return -1; char *tmp, *str = tgt->line.s; i = 0; while ( *str && i<3 ) { if ( *str=='\t' ) i++; str++; } *alt_freq = strtod(str, &tmp); if ( *tmp && !isspace(*tmp) ) { if ( str[0]=='.' && (!str[1] || isspace(str[1])) ) return -1; // missing value error("Could not parse: [%s]\n", tgt->line.s); } if ( *alt_freq<0 || *alt_freq>1 ) error("Could not parse AF: [%s]\n", tgt->line.s); return 0; } int estimate_AF(args_t *args, bcf1_t *line, double *alt_freq) { if ( !args->nitmp ) { args->nitmp = bcf_get_genotypes(args->hdr, line, &args->itmp, &args->mitmp); if ( args->nitmp != 2*args->nsmpl ) return -1; // not diploid? args->nitmp /= args->nsmpl; } int i, nalt = 0, nref = 0; for (i=0; insmpl; i++) { int32_t *gt = &args->itmp[i*args->nitmp]; if ( bcf_gt_is_missing(gt[0]) || bcf_gt_is_missing(gt[1]) ) continue; if ( bcf_gt_allele(gt[0]) ) nalt++; else nref++; if ( bcf_gt_allele(gt[1]) ) nalt++; else nref++; } if ( !nalt && !nref ) return -1; *alt_freq = (double)nalt / (nalt + nref); return 0; } int parse_line(args_t *args, bcf1_t *line, double *alt_freq, double *pdg) { args->nitmp = 0; // Set allele frequency int ret; if ( args->af_tag ) { // Use an INFO tag provided by the user ret = bcf_get_info_float(args->hdr, line, args->af_tag, &args->AFs, &args->mAFs); if ( ret==1 ) *alt_freq = args->AFs[0]; if ( ret==-2 ) error("Type mismatch for INFO/%s tag at %s:%d\n", args->af_tag, bcf_seqname(args->hdr,line), line->pos+1); } else if ( args->af_fname ) { // Read AF from a file ret = read_AF(args->files->targets, line, alt_freq); } else { // Use GTs or AC/AN: GTs when AC/AN not present or when GTs explicitly requested by --estimate-AF ret = -1; if ( !args->estimate_AF ) { int AC = -1, AN = 0; ret = bcf_get_info_int32(args->hdr, line, "AN", &args->itmp, &args->mitmp); if ( ret==1 ) { AN = args->itmp[0]; ret = bcf_get_info_int32(args->hdr, line, "AC", &args->itmp, &args->mitmp); if ( ret>0 ) AC = args->itmp[0]; } if ( AN<=0 || AC<0 ) ret = -1; else *alt_freq = (double) AC/AN; } if ( ret==-1 ) ret = estimate_AF(args, line, alt_freq); // reads GTs into args->itmp } if ( ret<0 ) return ret; // Set P(D|G) if ( args->fake_PLs ) { if ( !args->nitmp ) { args->nitmp = bcf_get_genotypes(args->hdr, line, &args->itmp, &args->mitmp); if ( args->nitmp != 2*args->nsmpl ) return -1; // not diploid? args->nitmp /= args->nsmpl; } int32_t *gt = &args->itmp[args->ismpl*args->nitmp]; if ( bcf_gt_is_missing(gt[0]) || bcf_gt_is_missing(gt[1]) ) return -1; int a = bcf_gt_allele(gt[0]); int b = bcf_gt_allele(gt[1]); if ( a!=b ) { pdg[0] = pdg[2] = args->unseen_PL; pdg[1] = 1 - 2*args->unseen_PL; } else if ( a==0 ) { pdg[0] = 1 - 2*args->unseen_PL; pdg[1] = pdg[2] = args->unseen_PL; } else { pdg[0] = pdg[1] = args->unseen_PL; pdg[2] = 1 - 2*args->unseen_PL; } } else { args->nitmp = bcf_get_format_int32(args->hdr, line, "PL", &args->itmp, &args->mitmp); if ( args->nitmp != args->nsmpl*line->n_allele*(line->n_allele+1)/2. ) return -1; // not diploid? args->nitmp /= args->nsmpl; int32_t *pl = &args->itmp[args->ismpl*args->nitmp]; pdg[0] = pl[0] < 256 ? args->pl2p[ pl[0] ] : 1.0; pdg[1] = pl[1] < 256 ? args->pl2p[ pl[1] ] : 1.0; pdg[2] = pl[2] < 256 ? args->pl2p[ pl[2] ] : 1.0; double sum = pdg[0] + pdg[1] + pdg[2]; if ( !sum ) return -1; pdg[0] /= sum; pdg[1] /= sum; pdg[2] /= sum; } return 0; } static void vcfroh(args_t *args, bcf1_t *line) { // Are we done? if ( !line ) { flush_viterbi(args); return; } args->ntot++; // Skip unwanted lines if ( line->rid == args->skip_rid ) return; if ( line->n_allele==1 ) return; // no ALT allele if ( line->n_allele!=2 ) return; // only biallelic sites if ( args->snps_only && !bcf_is_snp(line) ) return; // Initialize genetic map int skip_rid = 0; if ( args->prev_rid<0 ) { args->prev_rid = line->rid; args->prev_pos = line->pos; skip_rid = load_genmap(args, line); if ( !skip_rid && args->vi_training ) push_rid(args, line->rid); } // New chromosome? if ( args->prev_rid!=line->rid ) { skip_rid = load_genmap(args, line); if ( args->vi_training ) { if ( !skip_rid ) push_rid(args, line->rid); } else { flush_viterbi(args); args->nsites = 0; } args->prev_rid = line->rid; args->prev_pos = line->pos; } if ( skip_rid ) { fprintf(stderr,"Skipping the sequence, no genmap for %s\n", bcf_seqname(args->hdr,line)); args->skip_rid = line->rid; return; } if ( args->prev_pos > line->pos ) error("The file is not sorted?!\n"); args->prev_rid = line->rid; args->prev_pos = line->pos; // Ready for the new site int m = args->msites; hts_expand(uint32_t,args->nsites+1,args->msites,args->sites); if ( args->msites!=m ) args->eprob = (double*) realloc(args->eprob,sizeof(double)*args->msites*2); // Set likelihoods and alternate allele frequencies double alt_freq, pdg[3]; if ( parse_line(args, line, &alt_freq, pdg)<0 ) return; // something went wrong args->nused++; // Calculate emission probabilities P(D|AZ) and P(D|HW) double *eprob = &args->eprob[2*args->nsites]; eprob[STATE_AZ] = pdg[0]*(1-alt_freq) + pdg[2]*alt_freq; eprob[STATE_HW] = pdg[0]*(1-alt_freq)*(1-alt_freq) + 2*pdg[1]*(1-alt_freq)*alt_freq + pdg[2]*alt_freq*alt_freq; args->sites[args->nsites] = line->pos; args->nsites++; } static void usage(args_t *args) { fprintf(stderr, "\n"); fprintf(stderr, "About: HMM model for detecting runs of autozygosity.\n"); fprintf(stderr, "Usage: bcftools roh [options] \n"); fprintf(stderr, "\n"); fprintf(stderr, "General Options:\n"); fprintf(stderr, " --AF-tag use TAG for allele frequency\n"); fprintf(stderr, " --AF-file read allele frequencies from file (CHR\\tPOS\\tREF,ALT\\tAF)\n"); fprintf(stderr, " -e, --estimate-AF calculate AC,AN counts on the fly, using either all samples (\"-\") or samples listed in \n"); fprintf(stderr, " -G, --GTs-only use GTs, ignore PLs, use for PL of unseen genotypes. Safe value to use is 30 to account for GT errors.\n"); fprintf(stderr, " -I, --skip-indels skip indels as their genotypes are enriched for errors\n"); fprintf(stderr, " -m, --genetic-map genetic map in IMPUTE2 format, single file or mask, where string \"{CHROM}\" is replaced with chromosome name\n"); fprintf(stderr, " -M, --rec-rate constant recombination rate per bp\n"); fprintf(stderr, " -r, --regions restrict to comma-separated list of regions\n"); fprintf(stderr, " -R, --regions-file restrict to regions listed in a file\n"); fprintf(stderr, " -s, --sample sample to analyze\n"); fprintf(stderr, " -t, --targets similar to -r but streams rather than index-jumps\n"); fprintf(stderr, " -T, --targets-file similar to -R but streams rather than index-jumps\n"); fprintf(stderr, "\n"); fprintf(stderr, "HMM Options:\n"); fprintf(stderr, " -a, --hw-to-az P(AZ|HW) transition probability from AZ (autozygous) to HW (Hardy-Weinberg) state [1e-8]\n"); fprintf(stderr, " -H, --az-to-hw P(HW|AZ) transition probability from HW to AZ state [1e-7]\n"); fprintf(stderr, " -V, --viterbi-training perform Viterbi training to estimate transition probabilities\n"); fprintf(stderr, "\n"); exit(1); } int main_vcfroh(int argc, char *argv[]) { int c; args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->argc = argc; args->argv = argv; args->files = bcf_sr_init(); args->t2AZ = 1e-1; args->t2HW = 1e-1; args->rec_rate = 0; int regions_is_file = 0, targets_is_file = 0; static struct option loptions[] = { {"AF-tag",1,0,0}, {"AF-file",1,0,1}, {"estimate-AF",1,0,'e'}, {"GTs-only",1,0,'G'}, {"sample",1,0,'s'}, {"hw-to-az",1,0,'a'}, {"az-to-hw",1,0,'H'}, {"viterbi-training",0,0,'V'}, {"targets",1,0,'t'}, {"targets-file",1,0,'T'}, {"regions",1,0,'r'}, {"regions-file",1,0,'R'}, {"genetic-map",1,0,'m'}, {"rec-rate",1,0,'M'}, {"skip-indels",0,0,'I'}, {0,0,0,0} }; int naf_opts = 0; char *tmp; while ((c = getopt_long(argc, argv, "h?r:R:t:T:H:a:s:m:M:G:Ia:e:V",loptions,NULL)) >= 0) { switch (c) { case 0: args->af_tag = optarg; naf_opts++; break; case 1: args->af_fname = optarg; naf_opts++; break; case 'e': args->estimate_AF = optarg; naf_opts++; break; case 'I': args->snps_only = 1; break; case 'G': args->fake_PLs = 1; args->unseen_PL = strtod(optarg,&tmp); if ( *tmp ) error("Could not parse: -G %s\n", optarg); args->unseen_PL = pow(10,-args->unseen_PL/10.); break; case 'm': args->genmap_fname = optarg; break; case 'M': args->rec_rate = strtod(optarg,&tmp); if ( *tmp ) error("Could not parse: -M %s\n", optarg); break; case 's': args->sample = strdup(optarg); break; case 'a': args->t2AZ = strtod(optarg,&tmp); if ( *tmp ) error("Could not parse: -a %s\n", optarg); break; case 'H': args->t2HW = strtod(optarg,&tmp); if ( *tmp ) error("Could not parse: -H %s\n", optarg); break; case 't': args->targets_list = optarg; break; case 'T': args->targets_list = optarg; targets_is_file = 1; break; case 'r': args->regions_list = optarg; break; case 'R': args->regions_list = optarg; regions_is_file = 1; break; case 'V': args->vi_training = 1; break; case 'h': case '?': usage(args); break; default: error("Unknown argument: %s\n", optarg); } } if ( argct2AZ<0 || args->t2AZ>1 ) error("Error: The parameter --hw-to-az is not in [0,1]\n", args->t2AZ); if ( args->t2HW<0 || args->t2HW>1 ) error("Error: The parameter --az-to-hw is not in [0,1]\n", args->t2HW); if ( naf_opts>1 ) error("Error: The options --AF-tag, --AF-file and -e are mutually exclusive\n"); if ( args->af_fname && args->targets_list ) error("Error: The options --AF-file and -t are mutually exclusive\n"); if ( args->regions_list ) { if ( bcf_sr_set_regions(args->files, args->regions_list, regions_is_file)<0 ) error("Failed to read the regions: %s\n", args->regions_list); } if ( args->targets_list ) { if ( bcf_sr_set_targets(args->files, args->targets_list, targets_is_file, 0)<0 ) error("Failed to read the targets: %s\n", args->targets_list); } if ( args->af_fname ) { if ( bcf_sr_set_targets(args->files, args->af_fname, 1, 3)<0 ) error("Failed to read the targets: %s\n", args->af_fname); } if ( !bcf_sr_add_reader(args->files, argv[optind]) ) error("Failed to open %s: %s\n", argv[optind],bcf_sr_strerror(args->files->errnum)); init_data(args); while ( bcf_sr_next_line(args->files) ) { vcfroh(args, args->files->readers[0].buffer[0]); } vcfroh(args, NULL); fprintf(stderr,"Number of lines: total/processed: %d/%d\n", args->ntot,args->nused); destroy_data(args); free(args); return 0; } bcftools-1.2/vcfsom.c000066400000000000000000000564621246371514100146510ustar00rootroot00000000000000/* vcfsom.c -- SOM (Self-Organizing Map) filtering. Copyright (C) 2013-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include #include #include #include #include #include "bcftools.h" #define SOM_TRAIN 1 #define SOM_CLASSIFY 2 typedef struct { int ndim; // dimension of the map (2D, 3D, ...) int nbin; // number of bins in th map int size; // pow(nbin,ndim) int kdim; // dimension of the input vectors int nt, t; // total number of learning cycles and the current cycle double *w, *c; // weights and counts (sum of learning influence) double learn; // learning rate double bmu_th; // best-matching unit threshold int *a_idx, *b_idx; // temp arrays for traversing variable number of nested loops double *div; // dtto } som_t; typedef struct { // SOM parameters double bmu_th, learn; int ndim, nbin, ntrain, t; int nfold; // n-fold cross validation = the number of SOMs som_t **som; // annots reader's data htsFile *file; // reader kstring_t str; // temporary string for the reader int dclass, mvals; double *vals; // training data double *train_dat; int *train_class, mtrain_class, mtrain_dat; int rand_seed, good_class, bad_class; char **argv, *fname, *prefix; int argc, action, train_bad, merge; } args_t; static void usage(void); FILE *open_file(char **fname, const char *mode, const char *fmt, ...); void mkdir_p(const char *fmt, ...); char *msprintf(const char *fmt, ...) { va_list ap; va_start(ap, fmt); int n = vsnprintf(NULL, 0, fmt, ap) + 2; va_end(ap); char *str = (char*)malloc(n); va_start(ap, fmt); vsnprintf(str, n, fmt, ap); va_end(ap); return str; } /* * char *t, *p = str; * t = column_next(p, '\t'); * if ( strlen("")==t-p && !strncmp(p,"",t-p) ) printf("found!\n"); * * char *t; * t = column_next(str, '\t'); if ( !*t ) error("expected field\n", str); * t = column_next(t+1, '\t'); if ( !*t ) error("expected field\n", str); */ static inline char *column_next(char *start, char delim) { char *end = start; while (*end && *end!=delim) end++; return end; } /** * annots_reader_next() - reads next line from annots.tab.gz and sets: class, vals * Returns 1 on successful read or 0 if no further record could be read. */ int annots_reader_next(args_t *args) { args->str.l = 0; if ( hts_getline(args->file,'\n',&args->str)<=0 ) return 0; char *t, *line = args->str.s; if ( !args->mvals ) { t = line; while ( *t ) { if ( *t=='\t' ) args->mvals++; t++; } args->vals = (double*) malloc(args->mvals*sizeof(double)); } // class args->dclass = atoi(line); t = column_next(line, '\t'); // values int i; for (i=0; imvals; i++) { if ( !*t ) error("Could not parse %d-th data field: is the line truncated?\nThe line was: [%s]\n",i+2,line); args->vals[i] = atof(++t); t = column_next(t,'\t'); } return 1; } void annots_reader_reset(args_t *args) { if ( args->file ) hts_close(args->file); if ( !args->fname ) error("annots_reader_reset: no fname\n"); args->file = hts_open(args->fname, "r"); } void annots_reader_close(args_t *args) { hts_close(args->file); } static void som_write_map(char *prefix, som_t **som, int nsom) { FILE *fp = open_file(NULL,"w","%s.som",prefix); fwrite("SOMv1",5,1,fp); fwrite(&nsom,sizeof(int),1,fp); int i; for (i=0; isize,sizeof(int),1,fp); fwrite(&som[i]->kdim,sizeof(int),1,fp); fwrite(som[i]->w,sizeof(double),som[i]->size*som[i]->kdim,fp); fwrite(som[i]->c,sizeof(double),som[i]->size,fp); } if ( fclose(fp) ) error("%s.som: fclose failed\n",prefix); } static som_t** som_load_map(char *prefix, int *nsom) { FILE *fp = open_file(NULL,"r","%s.som",prefix); char buf[5]; if ( fread(buf,5,1,fp)!=1 || strncmp(buf,"SOMv1",5) ) error("Could not parse %s.som\n", prefix); if ( fread(nsom,sizeof(int),1,fp)!=1 ) error("Could not read %s.som\n", prefix); som_t **som = (som_t**)malloc(*nsom*sizeof(som_t*)); int i; for (i=0; i<*nsom; i++) { som[i] = (som_t*) calloc(1,sizeof(som_t)); if ( fread(&som[i]->size,sizeof(int),1,fp) != 1 ) error("Could not read %s.som\n", prefix); if ( fread(&som[i]->kdim,sizeof(int),1,fp) != 1 ) error("Could not read %s.som\n", prefix); som[i]->w = (double*) malloc(sizeof(double)*som[i]->size*som[i]->kdim); som[i]->c = (double*) malloc(sizeof(double)*som[i]->size); if ( fread(som[i]->w,sizeof(double),som[i]->size*som[i]->kdim,fp) != som[i]->size*som[i]->kdim ) error("Could not read from %s.som\n", prefix); if ( fread(som[i]->c,sizeof(double),som[i]->size,fp) != som[i]->size ) error("Could not read from %s.som\n", prefix); } if ( fclose(fp) ) error("%s.som: fclose failed\n",prefix); return som; } static void som_create_plot(som_t *som, char *prefix) { if ( som->ndim!=2 ) return; char *fname; FILE *fp = open_file(&fname,"w","%s.py",prefix); fprintf(fp, "import matplotlib as mpl\n" "mpl.use('Agg')\n" "import matplotlib.pyplot as plt\n" "\n" "dat = [\n" ); int i,j; double *val = som->c; for (i=0; inbin; i++) { fprintf(fp,"["); for (j=0; jnbin; j++) { if ( j>0 ) fprintf(fp,","); fprintf(fp,"%e", *val); val++; } fprintf(fp,"],\n"); } fprintf(fp, "]\n" "fig = plt.figure()\n" "ax1 = plt.subplot(111)\n" "im1 = ax1.imshow(dat)\n" "fig.colorbar(im1)\n" "plt.savefig('%s.png')\n" "plt.close()\n" "\n", prefix ); fclose(fp); free(fname); } // Find the best matching unit: the node with minimum distance from the input vector static inline int som_find_bmu(som_t *som, double *vec, double *dist) { double *ptr = som->w; double min_dist = HUGE_VAL; int min_idx = 0; int i, k; for (i=0; isize; i++) { double dist = 0; for (k=0; kkdim; k++) dist += (vec[k] - ptr[k]) * (vec[k] - ptr[k]); if ( dist < min_dist ) { min_dist = dist; min_idx = i; } ptr += som->kdim; } if ( dist ) *dist = min_dist; return min_idx; } static inline double som_get_score(som_t *som, double *vec, double bmu_th) { double *ptr = som->w; double min_dist = HUGE_VAL; int i, k; for (i=0; isize; i++) { if ( som->c[i] >= bmu_th ) { double dist = 0; for (k=0; kkdim; k++) dist += (vec[k] - ptr[k]) * (vec[k] - ptr[k]); if ( dist < min_dist ) min_dist = dist; } ptr += som->kdim; } return sqrt(min_dist); } // Convert flat index to that of a k-dimensional cube static inline void som_idx_to_ndim(som_t *som, int idx, int *ndim) { int i; double sub = 0; ndim[0] = idx/som->div[0]; for (i=1; indim; i++) { sub += ndim[i-1] * som->div[i-1]; ndim[i] = (idx - sub)/som->div[i]; } } static void som_train_site(som_t *som, double *vec, int update_counts) { // update learning rate and learning radius som->t++; double dt = exp(-som->t/som->nt); double learning_rate = som->learn * dt; double radius = som->nbin * dt; radius *= radius; // find the best matching unit and its indexes int min_idx = som_find_bmu(som, vec, NULL); som_idx_to_ndim(som, min_idx, som->a_idx); // update the weights: traverse the map and make all nodes within the // radius more similar to the input vector double *ptr = som->w; double *cnt = som->c; int i, j, k; for (i=0; isize; i++) { som_idx_to_ndim(som, i, som->b_idx); double dist = 0; for (j=0; jndim; j++) dist += (som->a_idx[j] - som->b_idx[j]) * (som->a_idx[j] - som->b_idx[j]); if ( dist <= radius ) { double influence = exp(-dist*dist*0.5/radius) * learning_rate; for (k=0; kkdim; k++) ptr[k] += influence * (vec[k] - ptr[k]); // Bad sites may help to shape the map, but only nodes with big enough // influence will be used for classification. if ( update_counts ) *cnt += influence; } ptr += som->kdim; cnt++; } } static void som_norm_counts(som_t *som) { int i; double max = 0; for (i=0; isize; i++) if ( max < som->c[i] ) max = som->c[i]; for (i=0; isize; i++) som->c[i] /= max; } static som_t *som_init(args_t *args) { som_t *som = (som_t*) calloc(1,sizeof(som_t)); som->ndim = args->ndim; som->nbin = args->nbin; som->kdim = args->mvals; som->nt = args->ntrain; som->learn = args->learn; som->bmu_th = args->bmu_th; som->size = pow(som->nbin,som->ndim); som->w = (double*) malloc(sizeof(double)*som->size*som->kdim); if ( !som->w ) error("Could not alloc %d bytes [nbin=%d ndim=%d kdim=%d]\n", sizeof(double)*som->size*som->kdim,som->nbin,som->ndim,som->kdim); som->c = (double*) calloc(som->size,sizeof(double)); if ( !som->w ) error("Could not alloc %d bytes [nbin=%d ndim=%d]\n", sizeof(double)*som->size,som->nbin,som->ndim); int i; for (i=0; isize*som->kdim; i++) som->w[i] = (double)random()/RAND_MAX; som->a_idx = (int*) malloc(sizeof(int)*som->ndim); som->b_idx = (int*) malloc(sizeof(int)*som->ndim); som->div = (double*) malloc(sizeof(double)*som->ndim); for (i=0; indim; i++) som->div[i] = pow(som->nbin,som->ndim-i-1); return som; } static void som_destroy(som_t *som) { free(som->a_idx); free(som->b_idx); free(som->div); free(som->w); free(som->c); free(som); } static void init_data(args_t *args) { // Get first line to learn the vector size annots_reader_reset(args); annots_reader_next(args); if ( args->action==SOM_CLASSIFY ) args->som = som_load_map(args->prefix,&args->nfold); } static void destroy_data(args_t *args) { int i; if ( args->som ) { for (i=0; infold; i++) som_destroy(args->som[i]); } free(args->train_dat); free(args->train_class); free(args->som); free(args->vals); free(args->str.s); } #define MERGE_MIN 0 #define MERGE_MAX 1 #define MERGE_AVG 2 static double get_min_score(args_t *args, int iskip) { int i; double score, min_score = HUGE_VAL; for (i=0; infold; i++) { if ( i==iskip ) continue; score = som_get_score(args->som[i], args->vals, args->bmu_th); if ( i==0 || score < min_score ) min_score = score; } return min_score; } static double get_max_score(args_t *args, int iskip) { int i; double score, max_score = -HUGE_VAL; for (i=0; infold; i++) { if ( i==iskip ) continue; score = som_get_score(args->som[i], args->vals, args->bmu_th); if ( i==0 || max_score < score ) max_score = score; } return max_score; } static double get_avg_score(args_t *args, int iskip) { int i, n = 0; double score = 0; for (i=0; infold; i++) { if ( i==iskip ) continue; score += som_get_score(args->som[i], args->vals, args->bmu_th); n++; } return score/n; } static int cmpfloat_desc(const void *a, const void *b) { float fa = *((float*)a); float fb = *((float*)b); if ( fafb ) return -1; return 0; } static void create_eval_plot(args_t *args) { FILE *fp = open_file(NULL,"w","%s.eval.py", args->prefix); fprintf(fp, "import matplotlib as mpl\n" "mpl.use('Agg')\n" "import matplotlib.pyplot as plt\n" "\n" "import csv\n" "csv.register_dialect('tab', delimiter='\\t', quoting=csv.QUOTE_NONE)\n" "dat = []\n" "with open('%s.eval', 'rb') as f:\n" "\treader = csv.reader(f, 'tab')\n" "\tfor row in reader:\n" "\t\tif row[0][0]!='#': dat.append(row)\n" "\n" "fig = plt.figure()\n" "ax1 = plt.subplot(111)\n" "ax1.plot([x[0] for x in dat],[x[1] for x in dat],'g',label='Good')\n" "ax1.plot([x[0] for x in dat],[x[2] for x in dat],'r',label='Bad')\n" "ax1.set_xlabel('SOM score')\n" "ax1.set_ylabel('Number of training sites')\n" "ax1.legend(loc='best',prop={'size':8},frameon=False)\n" "plt.savefig('%s.eval.png')\n" "plt.close()\n" "\n", args->prefix,args->prefix ); fclose(fp); } static void do_train(args_t *args) { // read training sites int i, igood = 0, ibad = 0, ngood = 0, nbad = 0, ntrain = 0; annots_reader_reset(args); while ( annots_reader_next(args) ) { // determine which of the nfold's SOMs to train int isom = 0; if ( args->dclass == args->good_class ) { if ( ++igood >= args->nfold ) igood = 0; isom = igood; ngood++; } else if ( args->dclass == args->bad_class ) { if ( ++ibad >= args->nfold ) ibad = 0; isom = ibad; nbad++; } else error("Could not determine the class: %d (vs %d and %d)\n", args->dclass,args->good_class,args->bad_class); // save the values for evaluation ntrain++; hts_expand(double, ntrain*args->mvals, args->mtrain_dat, args->train_dat); hts_expand(int, ntrain, args->mtrain_class, args->train_class); memcpy(args->train_dat+(ntrain-1)*args->mvals, args->vals, args->mvals*sizeof(double)); args->train_class[ntrain-1] = (args->dclass==args->good_class ? 1 : 0) | isom<<1; // store class + chunk used for training } annots_reader_close(args); // init maps if ( !args->ntrain ) args->ntrain = ngood/args->nfold; srandom(args->rand_seed); args->som = (som_t**) malloc(sizeof(som_t*)*args->nfold); for (i=0; infold; i++) args->som[i] = som_init(args); // train for (i=0; itrain_class[i] & 1; int isom = args->train_class[i] >> 1; if ( is_good || args->train_bad ) som_train_site(args->som[isom], args->train_dat+i*args->mvals, is_good); } // norm and create plots for (i=0; infold; i++) { som_norm_counts(args->som[i]); if ( args->prefix ) { char *bname = msprintf("%s.som.%d", args->prefix,i); som_create_plot(args->som[i], bname); free(bname); } } // evaluate float *good = (float*) malloc(sizeof(float)*ngood); assert(good); float *bad = (float*) malloc(sizeof(float)*nbad); assert(bad); igood = ibad = 0; double max_score = sqrt(args->som[0]->kdim); for (i=0; itrain_class[i] & 1; int isom = args->train_class[i] >> 1; // this vector was used for training isom-th SOM, skip if ( args->nfold==1 ) isom = -1; memcpy(args->vals, args->train_dat+i*args->mvals, args->mvals*sizeof(double)); switch (args->merge) { case MERGE_MIN: score = get_min_score(args, isom); break; case MERGE_MAX: score = get_max_score(args, isom); break; case MERGE_AVG: score = get_avg_score(args, isom); break; } score = 1.0 - score/max_score; if ( is_good ) good[igood++] = score; else bad[ibad++] = score; } qsort(good, ngood, sizeof(float), cmpfloat_desc); qsort(bad, nbad, sizeof(float), cmpfloat_desc); FILE *fp = NULL; if ( args->prefix ) fp = open_file(NULL,"w","%s.eval", args->prefix); igood = 0; ibad = 0; float prev_score = good[0]>bad[0] ? good[0] : bad[0]; int printed = 0; while ( igood 0.9 ) { printf("%.2f\t%.2f\t%e\t# %% of bad [1] and good [2] sites at a cutoff [3]\n", 100.*ibad/nbad,100.*igood/ngood,prev_score); printed = 1; } if ( igoodbad[ibad] ? good[igood] : bad[ibad]; else if ( igoodprefix,strerror(errno)); create_eval_plot(args); som_write_map(args->prefix, args->som, args->nfold); } free(good); free(bad); } static void do_classify(args_t *args) { annots_reader_reset(args); double max_score = sqrt(args->som[0]->kdim); while ( annots_reader_next(args) ) { double score = 0; switch (args->merge) { case MERGE_MIN: score = get_min_score(args, -1); break; case MERGE_MAX: score = get_max_score(args, -1); break; case MERGE_AVG: score = get_avg_score(args, -1); break; } printf("%e\n", 1.0 - score/max_score); } annots_reader_close(args); } static void usage(void) { fprintf(stderr, "\n"); fprintf(stderr, "About: SOM (Self-Organizing Map) filtering.\n"); fprintf(stderr, "Usage: bcftools som --train [options] \n"); fprintf(stderr, " bcftools som --classify [options]\n"); fprintf(stderr, "\n"); fprintf(stderr, "Model training options:\n"); fprintf(stderr, " -f, --nfold n-fold cross-validation (number of maps) [5]\n"); fprintf(stderr, " -p, --prefix prefix of output files\n"); fprintf(stderr, " -s, --size map size [20]\n"); fprintf(stderr, " -t, --train \n"); fprintf(stderr, "\n"); fprintf(stderr, "Classifying options:\n"); fprintf(stderr, " -c, --classify \n"); fprintf(stderr, "\n"); fprintf(stderr, "Experimental training options (no reason to change):\n"); fprintf(stderr, " -b, --bmu-threshold threshold for selection of best-matching unit [0.9]\n"); fprintf(stderr, " -d, --som-dimension SOM dimension [2]\n"); fprintf(stderr, " -e, --exclude-bad exclude bad sites from training, use for evaluation only\n"); fprintf(stderr, " -l, --learning-rate learning rate [1.0]\n"); fprintf(stderr, " -m, --merge -f merge algorithm [avg]\n"); fprintf(stderr, " -n, --ntrain-sites effective number of training sites [number of good sites]\n"); fprintf(stderr, " -r, --random-seed random seed, 0 for time() [1]\n"); fprintf(stderr, "\n"); exit(1); } int main_vcfsom(int argc, char *argv[]) { int c; args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->argc = argc; args->argv = argv; args->nbin = 20; args->learn = 1.0; args->bmu_th = 0.9; args->nfold = 5; args->rand_seed = 1; args->ndim = 2; args->bad_class = 1; args->good_class = 2; args->merge = MERGE_AVG; args->train_bad = 1; static struct option loptions[] = { {"help",0,0,'h'}, {"prefix",1,0,'p'}, {"ntrain-sites",1,0,'n'}, {"random-seed",1,0,'r'}, {"bmu-threshold",1,0,'b'}, {"exclude-bad",0,0,'e'}, {"learning-rate",1,0,'l'}, {"size",1,0,'s'}, {"som-dimension",1,0,'d'}, {"nfold",1,0,'f'}, {"merge",1,0,'m'}, {"train",0,0,'t'}, {"classify",0,0,'c'}, {0,0,0,0} }; while ((c = getopt_long(argc, argv, "htcp:n:r:b:l:s:f:d:m:e",loptions,NULL)) >= 0) { switch (c) { case 'e': args->train_bad = 0; break; case 'm': if ( !strcmp(optarg,"min") ) args->merge = MERGE_MIN; else if ( !strcmp(optarg,"max") ) args->merge = MERGE_MAX; else if ( !strcmp(optarg,"avg") ) args->merge = MERGE_AVG; else error("The -m method not recognised: %s\n", optarg); break; case 'p': args->prefix = optarg; break; case 'n': args->ntrain = atoi(optarg); break; case 'r': args->rand_seed = atoi(optarg); break; case 'b': args->bmu_th = atof(optarg); break; case 'l': args->learn = atof(optarg); break; case 's': args->nbin = atoi(optarg); break; case 'f': args->nfold = atoi(optarg); break; case 'd': args->ndim = atoi(optarg); if ( args->ndim<2 ) error("Expected -d >=2, got %d\n", args->ndim); if ( args->ndim>3 ) fprintf(stderr,"Warning: This will take a long time and is not going to make the results better: -d %d\n", args->ndim); break; case 't': args->action = SOM_TRAIN; break; case 'c': args->action = SOM_CLASSIFY; break; case 'h': case '?': usage(); default: error("Unknown argument: %s\n", optarg); } } if ( !args->rand_seed ) args->rand_seed = time(NULL); if ( argc!=optind+1 ) usage(); args->fname = argv[optind]; init_data(args); if ( args->action == SOM_TRAIN ) do_train(args); else if ( args->action == SOM_CLASSIFY ) do_classify(args); destroy_data(args); free(args); return 0; } bcftools-1.2/vcfstats.c000066400000000000000000001647421246371514100152120ustar00rootroot00000000000000/* vcfstats.c -- Produces stats which can be plotted using plot-vcfstats. Copyright (C) 2012-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ /* Notes and known issues: - SN ts/tv calculation includes all non-ref alleles listed in ALT while per-sample ts/tv takes the first non-ref allele only, something to consider with many non-ref HETs. */ #include #include #include #include #include #include #include #include #include #include #include "bcftools.h" #define HWE_STATS 1 #define QUAL_STATS 1 #define IRC_STATS 1 #define IRC_RLEN 10 typedef struct { char *tag; float min, max; uint64_t *vals_ts, *vals_tv; void *val; int nbins, type, m_val; } user_stats_t; typedef struct { int min, max, step, m_vals; uint64_t *vals; } idist_t; typedef struct { int n_snps, n_indels, n_mnps, n_others, n_mals, n_snp_mals, n_records; int *af_ts, *af_tv, *af_snps; // first bin of af_* stats are singletons #if HWE_STATS int *af_hwe; #endif #if IRC_STATS int n_repeat[IRC_RLEN][4], n_repeat_na; // number of indels which are repeat-consistent, repeat-inconsistent (dels and ins), and not applicable int *af_repeats[3]; #endif int ts_alt1, tv_alt1; #if QUAL_STATS int *qual_ts, *qual_tv, *qual_snps, *qual_indels; #endif int *insertions, *deletions, m_indel; // maximum indel length int in_frame, out_frame, na_frame, in_frame_alt1, out_frame_alt1, na_frame_alt1; int subst[15]; int *smpl_hets, *smpl_homRR, *smpl_homAA, *smpl_ts, *smpl_tv, *smpl_indels, *smpl_ndp, *smpl_sngl; int *smpl_frm_shifts; // not-applicable, in-frame, out-frame unsigned long int *smpl_dp; idist_t dp, dp_sites; int nusr; user_stats_t *usr; } stats_t; typedef struct { uint64_t m[3], mm[3]; // number of hom, het and non-ref hom matches and mismatches float r2sum; uint32_t r2n; } gtcmp_t; typedef struct { char *seq; int pos, cnt, len; } _idc1_t; typedef struct { faidx_t *ref; _idc1_t *dat; int ndat, mdat; } indel_ctx_t; typedef struct { // stats stats_t stats[3]; int *tmp_iaf, ntmp_iaf, m_af, m_qual, naf_hwe, mtmp_frm; uint8_t *tmp_frm; int dp_min, dp_max, dp_step; gtcmp_t *af_gts_snps, *af_gts_indels, *smpl_gts_snps, *smpl_gts_indels; // first bin of af_* stats are singletons // indel context indel_ctx_t *indel_ctx; char *ref_fname; // user stats int nusr; user_stats_t *usr; // other bcf_srs_t *files; bcf_sr_regions_t *exons; char **argv, *exons_fname, *regions_list, *samples_list, *targets_list; int argc, verbose_sites, first_allele_only, samples_is_file; int split_by_id, nstats; } args_t; static int type2dosage[6], type2ploidy[6], type2stats[6]; static void idist_init(idist_t *d, int min, int max, int step) { d->min = min; d->max = max; d->step = step; d->m_vals = 4 + (d->max - d->min)/d->step; d->vals = (uint64_t*) calloc(d->m_vals,sizeof(uint64_t)); } static void idist_destroy(idist_t *d) { if ( d->vals ) free(d->vals); } static inline uint64_t *idist(idist_t *d, int val) { if ( val < d->min ) return &d->vals[0]; if ( val > d->max ) return &d->vals[d->m_vals-1]; return &d->vals[1 + (val - d->min) / d->step]; } static inline int idist_i2bin(idist_t *d, int i) { if ( i<=0 ) return d->min; if ( i>= d->m_vals ) return d->max; return i-1+d->min; } #define IC_DBG 0 #if IC_DBG static void _indel_ctx_print1(_idc1_t *idc) { int i; fprintf(stdout, "%d\t", idc->cnt); for (i=0; ilen; i++) fputc(idc->seq[i], stdout); fputc('\n', stdout); } static void _indel_ctx_print(indel_ctx_t *ctx) { int i; for (i=0; indat; i++) _indel_ctx_print1(&ctx->dat[i]); fputc('\n',stdout); } #endif static int _indel_ctx_lookup(indel_ctx_t *ctx, char *seq, int seq_len, int *hit) { // binary search int min = 0, max = ctx->ndat - 1; while ( min<=max ) { int i = (min+max)/2; int cmp = strncmp(seq, ctx->dat[i].seq, seq_len); if ( cmp<0 ) max = i - 1; else if ( cmp>0 ) min = i + 1; else { if ( seq_len==ctx->dat[i].len ) { *hit = 1; return i; } else if ( seq_lendat[i].len ) max = i - 1; else min = i + 1; } } *hit = 0; return max; } static void _indel_ctx_insert(indel_ctx_t *ctx, char *seq, int seq_len, int pos) { int idat, hit, i; idat = _indel_ctx_lookup(ctx, seq, seq_len, &hit); if ( !hit ) { if ( pos>0 ) return; idat++; ctx->ndat++; hts_expand(_idc1_t, ctx->ndat+1, ctx->mdat, ctx->dat); if ( idatndat && ctx->ndat>1 ) memmove(&ctx->dat[idat+1], &ctx->dat[idat], (ctx->ndat - idat - 1)*sizeof(_idc1_t)); ctx->dat[idat].len = seq_len; ctx->dat[idat].cnt = 1; ctx->dat[idat].pos = pos; ctx->dat[idat].seq = (char*) malloc(sizeof(char)*(seq_len+1)); for (i=0; idat[idat].seq[i] = seq[i]; ctx->dat[idat].seq[i] = 0; return; } if ( ctx->dat[idat].pos + seq_len == pos ) { ctx->dat[idat].cnt++; ctx->dat[idat].pos = pos; } } indel_ctx_t *indel_ctx_init(char *fa_ref_fname) { indel_ctx_t *ctx = (indel_ctx_t *) calloc(1,sizeof(indel_ctx_t)); ctx->ref = fai_load(fa_ref_fname); if ( !ctx->ref ) { free(ctx); return NULL; } return ctx; } void indel_ctx_destroy(indel_ctx_t *ctx) { fai_destroy(ctx->ref); if ( ctx->mdat ) free(ctx->dat); free(ctx); } /** * indel_ctx_type() - determine indel context type * @ctx: * @chr: chromosome name * @pos: position of the first @ref base, 1-based * @ref: reference allele * @alt: alternate allele. Only first of multiple comma-separated alleles is * considered * @nrep: number of repeated elements (w) * @nlen: length of a single repeat element (w) * * Returns the INDEL length, negative for deletions, positive for insertions */ int indel_ctx_type(indel_ctx_t *ctx, char *chr, int pos, char *ref, char *alt, int *nrep, int *nlen) { const int win_size = 50; // hard-wired for now const int rep_len = IRC_RLEN; // hard-wired for now int ref_len = strlen(ref); int alt_len = 0; while ( alt[alt_len] && alt[alt_len]!=',' ) alt_len++; int i, fai_ref_len; char *fai_ref = faidx_fetch_seq(ctx->ref, chr, pos-1, pos+win_size, &fai_ref_len); for (i=0; i96 ) fai_ref[i] -= 32; // Sanity check: the reference sequence must match the REF allele for (i=0; indat = 0; for (i=0; indat; i++) { if ( max_cnt < ctx->dat[i].cnt || (max_cnt==ctx->dat[i].cnt && max_len < ctx->dat[i].len) ) { max_cnt = ctx->dat[i].cnt; max_len = ctx->dat[i].len; } free(ctx->dat[i].seq); } free(fai_ref); *nrep = max_cnt; *nlen = max_len; return alt_len - ref_len; } static void add_user_stats(args_t *args, char *str) { args->nusr++; args->usr = (user_stats_t*) realloc(args->usr,sizeof(user_stats_t)*args->nusr); user_stats_t *usr = &args->usr[args->nusr-1]; memset(usr,0,sizeof(*usr)); usr->min = 0; usr->max = 1; usr->nbins = 100; char *tmp = str; while ( *tmp && *tmp!=':' ) tmp++; usr->tag = (char*)calloc(tmp-str+2,sizeof(char)); memcpy(usr->tag,str,tmp-str); if ( *tmp ) { char *ptr = ++tmp; usr->min = strtod(tmp, &ptr); if ( tmp==ptr ) error("Could not parse %s\n", str); tmp = ptr+1; } if ( *tmp ) { char *ptr = tmp; usr->max = strtod(tmp, &ptr); if ( tmp==ptr ) error("Could not parse %s\n", str); tmp = ptr+1; } if ( *tmp ) { char *ptr = tmp; usr->nbins = strtol(tmp, &ptr, 10); if ( tmp==ptr ) error("Could not parse %s\n", str); if ( usr->nbins<=0 ) error("Number of bins does not make sense (%d): %s.\n", usr->nbins, str); } } static void init_user_stats(args_t *args, bcf_hdr_t *hdr, stats_t *stats) { stats->nusr = args->nusr; stats->usr = (user_stats_t*)malloc(sizeof(user_stats_t)*args->nusr); memcpy(stats->usr,args->usr,args->nusr*sizeof(user_stats_t)); int i; for (i=0; inusr; i++) { user_stats_t *usr = &stats->usr[i]; usr->vals_ts = (uint64_t*)calloc(usr->nbins,sizeof(uint64_t)); usr->vals_tv = (uint64_t*)calloc(usr->nbins,sizeof(uint64_t)); int id = bcf_hdr_id2int(hdr,BCF_DT_ID,usr->tag); if ( !bcf_hdr_idinfo_exists(hdr,BCF_HL_INFO,id) ) error("The INFO tag \"%s\" is not defined in the header\n", usr->tag); usr->type = bcf_hdr_id2type(hdr,BCF_HL_INFO,id); if ( usr->type!=BCF_HT_REAL && usr->type!=BCF_HT_INT ) error("The INFO tag \"%s\" is not of Float or Integer type (%d)\n", usr->type); } } static void init_stats(args_t *args) { int i; args->nstats = args->files->nreaders==1 ? 1 : 3; if ( args->split_by_id ) args->nstats = 2; // AF corresponds to AC but is more robust for mixture of haploid and diploid GTs args->m_af = 101; for (i=0; ifiles->nreaders; i++) if ( bcf_hdr_nsamples(args->files->readers[i].header) + 1> args->m_af ) args->m_af = bcf_hdr_nsamples(args->files->readers[i].header) + 1; #if QUAL_STATS args->m_qual = 999; #endif #if HWE_STATS args->naf_hwe = 100; #endif if ( args->samples_list ) { if ( !bcf_sr_set_samples(args->files,args->samples_list,args->samples_is_file) ) { if ( !bcf_hdr_nsamples(args->files->readers[0].header) ) error("No sample columns in %s\n", args->files->readers[0].fname); error("Unable to parse the samples: \"%s\"\n", args->samples_list); } args->af_gts_snps = (gtcmp_t *) calloc(args->m_af,sizeof(gtcmp_t)); args->af_gts_indels = (gtcmp_t *) calloc(args->m_af,sizeof(gtcmp_t)); args->smpl_gts_snps = (gtcmp_t *) calloc(args->files->n_smpl,sizeof(gtcmp_t)); args->smpl_gts_indels = (gtcmp_t *) calloc(args->files->n_smpl,sizeof(gtcmp_t)); } for (i=0; instats; i++) { stats_t *stats = &args->stats[i]; stats->m_indel = 60; stats->insertions = (int*) calloc(stats->m_indel,sizeof(int)); stats->deletions = (int*) calloc(stats->m_indel,sizeof(int)); stats->af_ts = (int*) calloc(args->m_af,sizeof(int)); stats->af_tv = (int*) calloc(args->m_af,sizeof(int)); stats->af_snps = (int*) calloc(args->m_af,sizeof(int)); int j; for (j=0; j<3; j++) stats->af_repeats[j] = (int*) calloc(args->m_af,sizeof(int)); #if QUAL_STATS stats->qual_ts = (int*) calloc(args->m_qual,sizeof(int)); stats->qual_tv = (int*) calloc(args->m_qual,sizeof(int)); stats->qual_snps = (int*) calloc(args->m_qual,sizeof(int)); stats->qual_indels = (int*) calloc(args->m_qual,sizeof(int)); #endif if ( args->files->n_smpl ) { stats->smpl_hets = (int *) calloc(args->files->n_smpl,sizeof(int)); stats->smpl_homAA = (int *) calloc(args->files->n_smpl,sizeof(int)); stats->smpl_homRR = (int *) calloc(args->files->n_smpl,sizeof(int)); stats->smpl_ts = (int *) calloc(args->files->n_smpl,sizeof(int)); stats->smpl_tv = (int *) calloc(args->files->n_smpl,sizeof(int)); stats->smpl_indels = (int *) calloc(args->files->n_smpl,sizeof(int)); stats->smpl_dp = (unsigned long int *) calloc(args->files->n_smpl,sizeof(unsigned long int)); stats->smpl_ndp = (int *) calloc(args->files->n_smpl,sizeof(int)); stats->smpl_sngl = (int *) calloc(args->files->n_smpl,sizeof(int)); #if HWE_STATS stats->af_hwe = (int*) calloc(args->m_af*args->naf_hwe,sizeof(int)); #endif if ( args->exons_fname ) stats->smpl_frm_shifts = (int*) calloc(args->files->n_smpl*3,sizeof(int)); } idist_init(&stats->dp, args->dp_min,args->dp_max,args->dp_step); idist_init(&stats->dp_sites, args->dp_min,args->dp_max,args->dp_step); init_user_stats(args, i!=1 ? args->files->readers[0].header : args->files->readers[1].header, stats); } if ( args->exons_fname ) { args->exons = bcf_sr_regions_init(args->exons_fname,1,0,1,2); if ( !args->exons ) error("Error occurred while reading, was the file compressed with bgzip: %s?\n", args->exons_fname); } #if IRC_STATS if ( args->ref_fname ) args->indel_ctx = indel_ctx_init(args->ref_fname); #endif type2dosage[GT_HOM_RR] = 0; type2dosage[GT_HET_RA] = 1; type2dosage[GT_HOM_AA] = 2; type2dosage[GT_HET_AA] = 2; type2dosage[GT_HAPL_R] = 0; type2dosage[GT_HAPL_A] = 1; type2ploidy[GT_HOM_RR] = 1; type2ploidy[GT_HET_RA] = 1; type2ploidy[GT_HOM_AA] = 1; type2ploidy[GT_HET_AA] = 1; type2ploidy[GT_HAPL_R] = -1; type2ploidy[GT_HAPL_A] = -1; type2stats[GT_HOM_RR] = 0; type2stats[GT_HET_RA] = 1; type2stats[GT_HOM_AA] = 2; type2stats[GT_HET_AA] = 1; type2stats[GT_HAPL_R] = 0; type2stats[GT_HAPL_A] = 2; } static void destroy_stats(args_t *args) { int id, j; for (id=0; idnstats; id++) { stats_t *stats = &args->stats[id]; if (stats->af_ts) free(stats->af_ts); if (stats->af_tv) free(stats->af_tv); if (stats->af_snps) free(stats->af_snps); for (j=0; j<3; j++) if (stats->af_repeats[j]) free(stats->af_repeats[j]); #if QUAL_STATS if (stats->qual_ts) free(stats->qual_ts); if (stats->qual_tv) free(stats->qual_tv); if (stats->qual_snps) free(stats->qual_snps); if (stats->qual_indels) free(stats->qual_indels); #endif #if HWE_STATS //if ( args->files->n_smpl ) free(stats->af_hwe); free(stats->af_hwe); #endif free(stats->insertions); free(stats->deletions); if (stats->smpl_hets) free(stats->smpl_hets); if (stats->smpl_homAA) free(stats->smpl_homAA); if (stats->smpl_homRR) free(stats->smpl_homRR); if (stats->smpl_ts) free(stats->smpl_ts); if (stats->smpl_tv) free(stats->smpl_tv); if (stats->smpl_indels) free(stats->smpl_indels); if (stats->smpl_dp) free(stats->smpl_dp); if (stats->smpl_ndp) free(stats->smpl_ndp); if (stats->smpl_sngl) free(stats->smpl_sngl); idist_destroy(&stats->dp); idist_destroy(&stats->dp_sites); for (j=0; jnusr; j++) { free(stats->usr[j].vals_ts); free(stats->usr[j].vals_tv); free(stats->usr[j].val); } free(stats->usr); if ( args->exons ) free(stats->smpl_frm_shifts); } for (j=0; jnusr; j++) free(args->usr[j].tag); free(args->usr); free(args->tmp_frm); if (args->tmp_iaf) free(args->tmp_iaf); if (args->exons) bcf_sr_regions_destroy(args->exons); if (args->af_gts_snps) free(args->af_gts_snps); if (args->af_gts_indels) free(args->af_gts_indels); if (args->smpl_gts_snps) free(args->smpl_gts_snps); if (args->smpl_gts_indels) free(args->smpl_gts_indels); if (args->indel_ctx) indel_ctx_destroy(args->indel_ctx); } static void init_iaf(args_t *args, bcf_sr_t *reader) { bcf1_t *line = reader->buffer[0]; if ( args->ntmp_iaf < line->n_allele ) { args->tmp_iaf = (int*)realloc(args->tmp_iaf, line->n_allele*sizeof(int)); args->ntmp_iaf = line->n_allele; } // tmp_iaf is first filled with AC counts in calc_ac and then transformed to // an index to af_gts_snps int i, ret = bcf_calc_ac(reader->header, line, args->tmp_iaf, args->samples_list ? BCF_UN_INFO|BCF_UN_FMT : BCF_UN_INFO); if ( ret ) { int an=0; for (i=0; in_allele; i++) an += args->tmp_iaf[i]; args->tmp_iaf[0] = 0; for (i=1; in_allele; i++) { if ( args->tmp_iaf[i]==1 ) args->tmp_iaf[i] = 0; // singletons into the first bin else if ( !an ) args->tmp_iaf[i] = 1; // no genotype at all, put to the AF=0 bin else args->tmp_iaf[i] = 1 + args->tmp_iaf[i] * (args->m_af-2.0) / an; } } else for (i=0; in_allele; i++) args->tmp_iaf[i] = 0; // todo: otherwise use AF } static inline void do_mnp_stats(args_t *args, stats_t *stats, bcf_sr_t *reader) { stats->n_mnps++; } static inline void do_other_stats(args_t *args, stats_t *stats, bcf_sr_t *reader) { stats->n_others++; } static void do_indel_stats(args_t *args, stats_t *stats, bcf_sr_t *reader) { stats->n_indels++; bcf1_t *line = reader->buffer[0]; #if QUAL_STATS int iqual = line->qual >= args->m_qual || isnan(line->qual) ? args->m_qual - 1 : line->qual; stats->qual_indels[iqual]++; #endif // Check if the indel is near an exon for the frameshift statistics int i, exon_overlap = 0; if ( args->exons ) { if ( !bcf_sr_regions_overlap(args->exons, bcf_seqname(reader->header,line),line->pos,line->pos) ) exon_overlap = 1; hts_expand(uint8_t,line->n_allele,args->mtmp_frm,args->tmp_frm); for (i=0; in_allele; i++) args->tmp_frm[i] = 0; } for (i=1; in_allele; i++) { if ( args->first_allele_only && i>1 ) break; if ( bcf_get_variant_type(line,i)!=VCF_INDEL ) continue; int len = line->d.var[i].n; #if IRC_STATS // Indel repeat consistency if ( args->indel_ctx ) { int nrep, nlen, ndel; ndel = indel_ctx_type(args->indel_ctx, (char*)reader->header->id[BCF_DT_CTG][line->rid].key, line->pos+1, line->d.allele[0], line->d.allele[i], &nrep, &nlen); if ( nlen<=1 || nrep<=1 ) { // not a repeat or a single base repeat stats->n_repeat_na++; stats->af_repeats[2][ args->tmp_iaf[i] ]++; } else { if ( abs(ndel) % nlen ) { // the length of the inserted/deleted sequence is not consistent with the repeat element stats->n_repeat[nlen-1][ndel<0 ? 1 : 3]++; stats->af_repeats[1][ args->tmp_iaf[i] ]++; } else { // the length consistent with the repeat stats->n_repeat[nlen-1][ndel<0 ? 0 : 2]++; stats->af_repeats[0][ args->tmp_iaf[i] ]++; } } } else stats->af_repeats[2][ args->tmp_iaf[i] ]++; #endif // Check the frameshifts int tlen = 0; if ( args->exons && exon_overlap ) // there is an exon { if ( len>0 ) { // insertion if ( args->exons->start <= line->pos && args->exons->end > line->pos ) tlen = abs(len); } else if ( args->exons->start <= line->pos + abs(len) ) { // deletion tlen = abs(len); if ( line->pos < args->exons->start ) // trim the beginning tlen -= args->exons->start - line->pos + 1; if ( args->exons->end < line->pos + abs(len) ) // trim the end tlen -= line->pos + abs(len) - args->exons->end; } } if ( tlen ) // there are some deleted/inserted bases in the exon { if ( tlen%3 ) { stats->out_frame++; args->tmp_frm[i] = 2; } else { stats->in_frame++; args->tmp_frm[i] = 1; } if ( i==1 ) { if ( tlen%3 ) stats->out_frame_alt1++; else stats->in_frame_alt1++; } } else // no exon affected { if ( i==1 ) stats->na_frame_alt1++; stats->na_frame++; } // Indel length distribution int *ptr = stats->insertions; if ( len<0 ) { len *= -1; ptr = stats->deletions; } if ( --len >= stats->m_indel ) len = stats->m_indel-1; ptr[len]++; } } static void do_user_stats(stats_t *stats, bcf_sr_t *reader, int is_ts) { int i; for (i=0; inusr; i++) { user_stats_t *usr = &stats->usr[i]; uint64_t *vals = is_ts ? usr->vals_ts : usr->vals_tv; float val; if ( usr->type==BCF_HT_REAL ) { if ( bcf_get_info_float(reader->header,reader->buffer[0],usr->tag,&usr->val,&usr->m_val)<=0 ) continue; val = ((float*)usr->val)[0]; } else { if ( bcf_get_info_int32(reader->header,reader->buffer[0],usr->tag,&usr->val,&usr->m_val)<=0 ) continue; val = ((int32_t*)usr->val)[0]; } int idx; if ( val<=usr->min ) idx = 0; else if ( val>=usr->max ) idx = usr->nbins - 1; else idx = (val - usr->min)/(usr->max - usr->min) * (usr->nbins-1); vals[idx]++; } } static void do_snp_stats(args_t *args, stats_t *stats, bcf_sr_t *reader) { stats->n_snps++; bcf1_t *line = reader->buffer[0]; int ref = bcf_acgt2int(*line->d.allele[0]); if ( ref<0 ) return; #if QUAL_STATS int iqual = line->qual >= args->m_qual || isnan(line->qual) ? args->m_qual - 1 : line->qual; stats->qual_snps[iqual]++; #endif int i; for (i=1; in_allele; i++) { if ( args->first_allele_only && i>1 ) break; if ( !(bcf_get_variant_type(line,i)&VCF_SNP) ) continue; int alt = bcf_acgt2int(*line->d.allele[i]); if ( alt<0 || ref==alt ) continue; stats->subst[ref<<2|alt]++; int iaf = args->tmp_iaf[i]; stats->af_snps[iaf]++; if ( abs(ref-alt)==2 ) { if (i==1) { stats->ts_alt1++; #if QUAL_STATS stats->qual_ts[iqual]++; #endif do_user_stats(stats, reader, 1); } stats->af_ts[iaf]++; } else { if (i==1) { stats->tv_alt1++; #if QUAL_STATS stats->qual_tv[iqual]++; #endif do_user_stats(stats, reader, 0); } stats->af_tv[iaf]++; } } } static void do_sample_stats(args_t *args, stats_t *stats, bcf_sr_t *reader, int matched) { bcf_srs_t *files = args->files; bcf1_t *line = reader->buffer[0]; bcf_fmt_t *fmt_ptr; int nref_tot = 0, nhet_tot = 0, nalt_tot = 0; int line_type = bcf_get_variant_types(line); if ( (fmt_ptr = bcf_get_fmt(reader->header,reader->buffer[0],"GT")) ) { int ref = bcf_acgt2int(*line->d.allele[0]); int is, n_nref = 0, i_nref = 0; for (is=0; isfiles->n_smpl; is++) { int ial, jal; int gt = bcf_gt_type(fmt_ptr, reader->samples[is], &ial, &jal); if ( gt==GT_UNKN ) continue; if ( gt==GT_HAPL_R || gt==GT_HAPL_A ) { if ( line_type&VCF_INDEL && stats->smpl_frm_shifts ) { assert( ialn_allele ); stats->smpl_frm_shifts[is*3 + args->tmp_frm[ial]]++; } continue; } if ( gt != GT_HOM_RR ) { n_nref++; i_nref = is; } #if HWE_STATS switch (gt) { case GT_HOM_RR: nref_tot++; break; case GT_HET_RA: nhet_tot++; break; case GT_HET_AA: case GT_HOM_AA: nalt_tot++; break; } #endif if ( line_type&VCF_SNP ) { if ( gt == GT_HET_RA ) stats->smpl_hets[is]++; else if ( gt == GT_HET_AA ) stats->smpl_hets[is]++; else if ( gt == GT_HOM_RR ) stats->smpl_homRR[is]++; else if ( gt == GT_HOM_AA ) stats->smpl_homAA[is]++; if ( gt != GT_HOM_RR && line->d.var[ial].type&VCF_SNP ) // this is safe, bcf_get_variant_types has been already called { int alt = bcf_acgt2int(*line->d.allele[ial]); if ( alt<0 ) continue; if ( abs(ref-alt)==2 ) stats->smpl_ts[is]++; else stats->smpl_tv[is]++; } } if ( line_type&VCF_INDEL ) { if ( gt != GT_HOM_RR ) stats->smpl_indels[is]++; if ( stats->smpl_frm_shifts ) { assert( ialn_allele && jaln_allele ); stats->smpl_frm_shifts[is*3 + args->tmp_frm[ial]]++; stats->smpl_frm_shifts[is*3 + args->tmp_frm[jal]]++; } } } if ( n_nref==1 ) stats->smpl_sngl[i_nref]++; } #if HWE_STATS if ( nhet_tot + nref_tot + nalt_tot ) { float het_frac = (float)nhet_tot/(nhet_tot + nref_tot + nalt_tot); int idx = het_frac*(args->naf_hwe - 1); if ( line->n_allele>1 ) idx += args->naf_hwe*args->tmp_iaf[1]; stats->af_hwe[idx]++; } #endif if ( (fmt_ptr = bcf_get_fmt(reader->header,reader->buffer[0],"DP")) ) { #define BRANCH_INT(type_t,missing,vector_end) { \ int is; \ for (is=0; isfiles->n_smpl; is++) \ { \ type_t *p = (type_t *) (fmt_ptr->p + fmt_ptr->size*is); \ if ( *p==vector_end ) continue; \ if ( *p!=missing ) \ { \ (*idist(&stats->dp, *p))++; \ stats->smpl_ndp[is]++; \ stats->smpl_dp[is] += *p; \ } \ } \ } switch (fmt_ptr->type) { case BCF_BT_INT8: BRANCH_INT(int8_t, bcf_int8_missing, bcf_int8_vector_end); break; case BCF_BT_INT16: BRANCH_INT(int16_t, bcf_int16_missing, bcf_int16_vector_end); break; case BCF_BT_INT32: BRANCH_INT(int32_t, bcf_int32_missing, bcf_int32_vector_end); break; default: fprintf(stderr, "[E::%s] todo: %d\n", __func__, fmt_ptr->type); exit(1); break; } #undef BRANCH_INT } if ( matched==3 ) { int is; bcf_fmt_t *fmt0, *fmt1; fmt0 = bcf_get_fmt(files->readers[0].header,files->readers[0].buffer[0],"GT"); if ( !fmt0 ) return; fmt1 = bcf_get_fmt(files->readers[1].header,files->readers[1].buffer[0],"GT"); if ( !fmt1 ) return; // only the first ALT allele is considered int iaf = line->n_allele>1 ? args->tmp_iaf[1] : 1; int line_type = bcf_get_variant_types(files->readers[0].buffer[0]); gtcmp_t *af_stats = line_type&VCF_SNP ? args->af_gts_snps : args->af_gts_indels; gtcmp_t *smpl_stats = line_type&VCF_SNP ? args->smpl_gts_snps : args->smpl_gts_indels; int r2n = 0; float x = 0, y = 0, xy = 0, x2 = 0, y2 = 0; for (is=0; isn_smpl; is++) { // Simplified comparison: only 0/0, 0/1, 1/1 is looked at as the identity of // actual alleles can be enforced by running without the -c option. int gt0 = bcf_gt_type(fmt0, files->readers[0].samples[is], NULL, NULL); if ( gt0 == GT_UNKN ) continue; int gt1 = bcf_gt_type(fmt1, files->readers[1].samples[is], NULL, NULL); if ( gt1 == GT_UNKN ) continue; if ( type2ploidy[gt0]*type2ploidy[gt1] == -1 ) continue; // cannot compare diploid and haploid genotypes int dsg0 = type2dosage[gt0]; int dsg1 = type2dosage[gt1]; x += dsg0; x2 += dsg0*dsg0; y += dsg1; y2 += dsg1*dsg1; xy += dsg0*dsg1; r2n++; int idx = type2stats[gt0]; if ( gt0==gt1 ) { af_stats[iaf].m[idx]++; smpl_stats[is].m[idx]++; } else { af_stats[iaf].mm[idx]++; smpl_stats[is].mm[idx]++; } } if ( r2n ) { x /= r2n; y /= r2n; x2 /= r2n; y2 /= r2n; xy /= r2n; float cov = xy - x*y; float var2 = (x2 - x*x) * (y2 - y*y); if ( var2!=0 ) { af_stats[iaf].r2sum += cov*cov/var2; af_stats[iaf].r2n++; } } if ( args->verbose_sites ) { int nm = 0, nmm = 0, nrefm = 0; for (is=0; isn_smpl; is++) { int gt = bcf_gt_type(fmt0, files->readers[0].samples[is], NULL, NULL); if ( gt == GT_UNKN ) continue; int gt2 = bcf_gt_type(fmt1, files->readers[1].samples[is], NULL, NULL); if ( gt2 == GT_UNKN ) continue; if ( gt != gt2 ) { nmm++; bcf_sr_t *reader = &files->readers[0]; printf("DBG\t%s\t%d\t%s\t%d\t%d\n",reader->header->id[BCF_DT_CTG][reader->buffer[0]->rid].key,reader->buffer[0]->pos+1,files->samples[is],gt,gt2); } else { if ( gt!=GT_HOM_RR ) nrefm++; nm++; } } float nrd = nrefm+nmm ? 100.*nmm/(nrefm+nmm) : 0; printf("PSD\t%s\t%d\t%d\t%d\t%f\n", reader->header->id[BCF_DT_CTG][reader->buffer[0]->rid].key,reader->buffer[0]->pos+1,nm,nmm,nrd); } } } static void do_vcf_stats(args_t *args) { bcf_srs_t *files = args->files; assert( sizeof(int)>files->nreaders ); while ( bcf_sr_next_line(files) ) { bcf_sr_t *reader = NULL; bcf1_t *line = NULL; int ret = 0, i; for (i=0; inreaders; i++) { if ( !bcf_sr_has_line(files,i) ) continue; ret |= 1<readers[i]; line = files->readers[i].buffer[0]; } int line_type = bcf_get_variant_types(line); init_iaf(args, reader); stats_t *stats = &args->stats[ret-1]; if ( args->split_by_id && line->d.id[0]=='.' && !line->d.id[1] ) stats = &args->stats[1]; stats->n_records++; if ( line_type&VCF_SNP ) do_snp_stats(args, stats, reader); if ( line_type&VCF_INDEL ) do_indel_stats(args, stats, reader); if ( line_type&VCF_MNP ) do_mnp_stats(args, stats, reader); if ( line_type&VCF_OTHER ) do_other_stats(args, stats, reader); if ( line->n_allele>2 ) { stats->n_mals++; if ( line_type == VCF_SNP ) stats->n_snp_mals++; } if ( files->n_smpl ) do_sample_stats(args, stats, reader, ret); if ( bcf_get_info_int32(reader->header,line,"DP",&args->tmp_iaf,&args->ntmp_iaf)==1 ) (*idist(&stats->dp_sites, args->tmp_iaf[0]))++; } } static void print_header(args_t *args) { int i; printf("# This file was produced by bcftools stats (%s+htslib-%s) and can be plotted using plot-vcfstats.\n", bcftools_version(),hts_version()); printf("# The command line was:\tbcftools %s ", args->argv[0]); for (i=1; iargc; i++) printf(" %s",args->argv[i]); printf("\n#\n"); printf("# Definition of sets:\n# ID\t[2]id\t[3]tab-separated file names\n"); if ( args->files->nreaders==1 ) { const char *fname = strcmp("-",args->files->readers[0].fname) ? args->files->readers[0].fname : ""; if ( args->split_by_id ) { printf("ID\t0\t%s:known (sites with ID different from \".\")\n", fname); printf("ID\t1\t%s:novel (sites where ID column is \".\")\n", fname); } else printf("ID\t0\t%s\n", fname); } else { const char *fname0 = strcmp("-",args->files->readers[0].fname) ? args->files->readers[0].fname : ""; const char *fname1 = strcmp("-",args->files->readers[1].fname) ? args->files->readers[1].fname : ""; printf("ID\t0\t%s\n", fname0); printf("ID\t1\t%s\n", fname1); printf("ID\t2\t%s\t%s\n", fname0,fname1); if ( args->verbose_sites ) { printf( "# Verbose per-site discordance output.\n" "# PSD\t[2]CHROM\t[3]POS\t[4]Number of matches\t[5]Number of mismatches\t[6]NRD\n"); printf( "# Verbose per-site and per-sample output. Genotype codes: %d:HomRefRef, %d:HomAltAlt, %d:HetAltRef, %d:HetAltAlt, %d:haploidRef, %d:haploidAlt\n" "# DBG\t[2]CHROM\t[3]POS\t[4]Sample\t[5]GT in %s\t[6]GT in %s\n", GT_HOM_RR, GT_HOM_AA, GT_HET_RA, GT_HET_AA, GT_HAPL_R, GT_HAPL_A, fname0,fname1); } } } #define T2S(x) type2stats[x] static void print_stats(args_t *args) { int i, id; printf("# SN, Summary numbers:\n# SN\t[2]id\t[3]key\t[4]value\n"); for (id=0; idfiles->nreaders; id++) printf("SN\t%d\tnumber of samples:\t%d\n", id, bcf_hdr_nsamples(args->files->readers[id].header)); for (id=0; idnstats; id++) { stats_t *stats = &args->stats[id]; printf("SN\t%d\tnumber of records:\t%d\n", id, stats->n_records); printf("SN\t%d\tnumber of SNPs:\t%d\n", id, stats->n_snps); printf("SN\t%d\tnumber of MNPs:\t%d\n", id, stats->n_mnps); printf("SN\t%d\tnumber of indels:\t%d\n", id, stats->n_indels); printf("SN\t%d\tnumber of others:\t%d\n", id, stats->n_others); printf("SN\t%d\tnumber of multiallelic sites:\t%d\n", id, stats->n_mals); printf("SN\t%d\tnumber of multiallelic SNP sites:\t%d\n", id, stats->n_snp_mals); } printf("# TSTV, transitions/transversions:\n# TSTV\t[2]id\t[3]ts\t[4]tv\t[5]ts/tv\t[6]ts (1st ALT)\t[7]tv (1st ALT)\t[8]ts/tv (1st ALT)\n"); for (id=0; idnstats; id++) { stats_t *stats = &args->stats[id]; int ts=0,tv=0; for (i=0; im_af; i++) { ts += stats->af_ts[i]; tv += stats->af_tv[i]; } printf("TSTV\t%d\t%d\t%d\t%.2f\t%d\t%d\t%.2f\n", id,ts,tv,tv?(float)ts/tv:0, stats->ts_alt1,stats->tv_alt1,stats->tv_alt1?(float)stats->ts_alt1/stats->tv_alt1:0); } if ( args->exons_fname ) { printf("# FS, Indel frameshifts:\n# FS\t[2]id\t[3]in-frame\t[4]out-frame\t[5]not applicable\t[6]out/(in+out) ratio\t[7]in-frame (1st ALT)\t[8]out-frame (1st ALT)\t[9]not applicable (1st ALT)\t[10]out/(in+out) ratio (1st ALT)\n"); for (id=0; idnstats; id++) { int in=args->stats[id].in_frame, out=args->stats[id].out_frame, na=args->stats[id].na_frame; int in1=args->stats[id].in_frame_alt1, out1=args->stats[id].out_frame_alt1, na1=args->stats[id].na_frame_alt1; printf("FS\t%d\t%d\t%d\t%d\t%.2f\t%d\t%d\t%d\t%.2f\n", id, in,out,na,out?(float)out/(in+out):0,in1,out1,na1,out1?(float)out1/(in1+out1):0); } } if ( args->indel_ctx ) { printf("# ICS, Indel context summary:\n# ICS\t[2]id\t[3]repeat-consistent\t[4]repeat-inconsistent\t[5]not applicable\t[6]c/(c+i) ratio\n"); for (id=0; idnstats; id++) { int nc = 0, ni = 0, na = args->stats[id].n_repeat_na; for (i=0; istats[id].n_repeat[i][0] + args->stats[id].n_repeat[i][2]; ni += args->stats[id].n_repeat[i][1] + args->stats[id].n_repeat[i][3]; } printf("ICS\t%d\t%d\t%d\t%d\t%.4f\n", id, nc,ni,na,nc+ni ? (float)nc/(nc+ni) : 0.0); } printf("# ICL, Indel context by length:\n# ICL\t[2]id\t[3]length of repeat element\t[4]repeat-consistent deletions)\t[5]repeat-inconsistent deletions\t[6]consistent insertions\t[7]inconsistent insertions\t[8]c/(c+i) ratio\n"); for (id=0; idnstats; id++) { for (i=1; istats[id].n_repeat[i][0]+args->stats[id].n_repeat[i][2], ni = args->stats[id].n_repeat[i][1]+args->stats[id].n_repeat[i][3]; printf("ICL\t%d\t%d\t%d\t%d\t%d\t%d\t%.4f\n", id, i+1, args->stats[id].n_repeat[i][0],args->stats[id].n_repeat[i][1],args->stats[id].n_repeat[i][2],args->stats[id].n_repeat[i][3], nc+ni ? (float)nc/(nc+ni) : 0.0); } } } printf("# SiS, Singleton stats:\n# SiS\t[2]id\t[3]allele count\t[4]number of SNPs\t[5]number of transitions\t[6]number of transversions\t[7]number of indels\t[8]repeat-consistent\t[9]repeat-inconsistent\t[10]not applicable\n"); for (id=0; idnstats; id++) { stats_t *stats = &args->stats[id]; printf("SiS\t%d\t%d\t%d\t%d\t%d\t%d\t%d\t%d\t%d\n", id,1,stats->af_snps[0],stats->af_ts[0],stats->af_tv[0], stats->af_repeats[0][0]+stats->af_repeats[1][0]+stats->af_repeats[2][0],stats->af_repeats[0][0],stats->af_repeats[1][0],stats->af_repeats[2][0]); // put the singletons stats into the first AF bin, note that not all of the stats is transferred (i.e. nrd mismatches) stats->af_snps[1] += stats->af_snps[0]; stats->af_ts[1] += stats->af_ts[0]; stats->af_tv[1] += stats->af_tv[0]; stats->af_repeats[0][1] += stats->af_repeats[0][0]; stats->af_repeats[1][1] += stats->af_repeats[1][0]; stats->af_repeats[2][1] += stats->af_repeats[2][0]; } printf("# AF, Stats by non-reference allele frequency:\n# AF\t[2]id\t[3]allele frequency\t[4]number of SNPs\t[5]number of transitions\t[6]number of transversions\t[7]number of indels\t[8]repeat-consistent\t[9]repeat-inconsistent\t[10]not applicable\n"); for (id=0; idnstats; id++) { stats_t *stats = &args->stats[id]; for (i=1; im_af; i++) // note that af[1] now contains also af[0], see SiS stats output above { if ( stats->af_snps[i]+stats->af_ts[i]+stats->af_tv[i]+stats->af_repeats[0][i]+stats->af_repeats[1][i]+stats->af_repeats[2][i] == 0 ) continue; printf("AF\t%d\t%f\t%d\t%d\t%d\t%d\t%d\t%d\t%d\n", id,100.*(i-1)/(args->m_af-1),stats->af_snps[i],stats->af_ts[i],stats->af_tv[i], stats->af_repeats[0][i]+stats->af_repeats[1][i]+stats->af_repeats[2][i],stats->af_repeats[0][i],stats->af_repeats[1][i],stats->af_repeats[2][i]); } } #if QUAL_STATS printf("# QUAL, Stats by quality:\n# QUAL\t[2]id\t[3]Quality\t[4]number of SNPs\t[5]number of transitions (1st ALT)\t[6]number of transversions (1st ALT)\t[7]number of indels\n"); for (id=0; idnstats; id++) { stats_t *stats = &args->stats[id]; for (i=0; im_qual; i++) { if ( stats->qual_snps[i]+stats->qual_ts[i]+stats->qual_tv[i]+stats->qual_indels[i] == 0 ) continue; printf("QUAL\t%d\t%d\t%d\t%d\t%d\t%d\n", id,i,stats->qual_snps[i],stats->qual_ts[i],stats->qual_tv[i],stats->qual_indels[i]); } } #endif for (i=0; inusr; i++) { printf("# USR:%s, Stats by %s:\n# USR:%s\t[2]id\t[3]%s\t[4]number of SNPs\t[5]number of transitions (1st ALT)\t[6]number of transversions (1st ALT)\n", args->usr[i].tag,args->usr[i].tag,args->usr[i].tag,args->usr[i].tag); for (id=0; idnstats; id++) { user_stats_t *usr = &args->stats[id].usr[i]; int j; for (j=0; jnbins; j++) { if ( usr->vals_ts[j]+usr->vals_tv[j] == 0 ) continue; // skip empty bins float val = usr->min + (usr->max - usr->min)*j/(usr->nbins-1); const char *fmt = usr->type==BCF_HT_REAL ? "USR:%s\t%d\t%e\t%d\t%d\t%d\n" : "USR:%s\t%d\t%.0f\t%d\t%d\t%d\n"; printf(fmt,usr->tag,id,val,usr->vals_ts[j]+usr->vals_tv[j],usr->vals_ts[j],usr->vals_tv[j]); } } } printf("# IDD, InDel distribution:\n# IDD\t[2]id\t[3]length (deletions negative)\t[4]count\n"); for (id=0; idnstats; id++) { stats_t *stats = &args->stats[id]; for (i=stats->m_indel-1; i>=0; i--) if ( stats->deletions[i] ) printf("IDD\t%d\t%d\t%d\n", id,-i-1,stats->deletions[i]); for (i=0; im_indel; i++) if ( stats->insertions[i] ) printf("IDD\t%d\t%d\t%d\n", id,i+1,stats->insertions[i]); } printf("# ST, Substitution types:\n# ST\t[2]id\t[3]type\t[4]count\n"); for (id=0; idnstats; id++) { int t; for (t=0; t<15; t++) { if ( t>>2 == (t&3) ) continue; printf("ST\t%d\t%c>%c\t%d\n", id, bcf_int2acgt(t>>2),bcf_int2acgt(t&3),args->stats[id].subst[t]); } } if ( args->files->nreaders>1 && args->files->n_smpl ) { printf("SN\t%d\tnumber of samples:\t%d\n", 2, args->files->n_smpl); int x; for (x=0; x<2; x++) { gtcmp_t *stats; if ( x==0 ) { printf("# GCsAF, Genotype concordance by non-reference allele frequency (SNPs)\n# GCsAF\t[2]id\t[3]allele frequency\t[4]RR Hom matches\t[5]RA Het matches\t[6]AA Hom matches\t[7]RR Hom mismatches\t[8]RA Het mismatches\t[9]AA Hom mismatches\t[10]dosage r-squared\t[11]number of sites\n"); stats = args->af_gts_snps; } else { printf("# GCiAF, Genotype concordance by non-reference allele frequency (indels)\n# GCiAF\t[2]id\t[3]allele frequency\t[4]RR Hom matches\t[5]RA Het matches\t[6]AA Hom matches\t[7]RR Hom mismatches\t[8]RA Het mismatches\t[9]AA Hom mismatches\t[10]dosage r-squared\t[11]number of sites\n"); stats = args->af_gts_indels; } uint64_t nrd_m[3] = {0,0,0}, nrd_mm[3] = {0,0,0}; for (i=0; im_af; i++) { int j, n = 0; for (j=0; j<3; j++) { n += stats[i].m[j] + stats[i].mm[j]; nrd_m[j] += stats[i].m[j]; nrd_mm[j] += stats[i].mm[j]; } if ( !i || !n ) continue; // skip singleton stats and empty bins printf("GC%cAF\t2\t%f", x==0 ? 's' : 'i', 100.*(i-1)/(args->m_af-1)); printf("\t%"PRId64"\t%"PRId64"\t%"PRId64"", stats[i].m[T2S(GT_HOM_RR)],stats[i].m[T2S(GT_HET_RA)],stats[i].m[T2S(GT_HOM_AA)]); printf("\t%"PRId64"\t%"PRId64"\t%"PRId64"", stats[i].mm[T2S(GT_HOM_RR)],stats[i].mm[T2S(GT_HET_RA)],stats[i].mm[T2S(GT_HOM_AA)]); printf("\t%f\t%"PRId32"\n", stats[i].r2n ? stats[i].r2sum/stats[i].r2n : -1.0, stats[i].r2n); } if ( x==0 ) { printf("# NRD and discordance is calculated as follows:\n"); printf("# m .. number of matches\n"); printf("# x .. number of mismatches\n"); printf("# NRD = (xRR + xRA + xAA) / (xRR + xRA + xAA + mRA + mAA)\n"); printf("# RR discordance = xRR / (xRR + mRR)\n"); printf("# RA discordance = xRA / (xRA + mRA)\n"); printf("# AA discordance = xAA / (xAA + mAA)\n"); printf("# Non-Reference Discordance (NRD), SNPs\n# NRDs\t[2]id\t[3]NRD\t[4]Ref/Ref discordance\t[5]Ref/Alt discordance\t[6]Alt/Alt discordance\n"); } else printf("# Non-Reference Discordance (NRD), indels\n# NRDi\t[2]id\t[3]NRD\t[4]Ref/Ref discordance\t[5]Ref/Alt discordance\t[6]Alt/Alt discordance\n"); uint64_t m = nrd_m[T2S(GT_HET_RA)] + nrd_m[T2S(GT_HOM_AA)]; uint64_t mm = nrd_mm[T2S(GT_HOM_RR)] + nrd_mm[T2S(GT_HET_RA)] + nrd_mm[T2S(GT_HOM_AA)]; printf("NRD%c\t2\t%f\t%f\t%f\t%f\n", x==0 ? 's' : 'i', m+mm ? mm*100.0/(m+mm) : 0, nrd_m[T2S(GT_HOM_RR)]+nrd_mm[T2S(GT_HOM_RR)] ? nrd_mm[T2S(GT_HOM_RR)]*100.0/(nrd_m[T2S(GT_HOM_RR)]+nrd_mm[T2S(GT_HOM_RR)]) : 0, nrd_m[T2S(GT_HET_RA)]+nrd_mm[T2S(GT_HET_RA)] ? nrd_mm[T2S(GT_HET_RA)]*100.0/(nrd_m[T2S(GT_HET_RA)]+nrd_mm[T2S(GT_HET_RA)]) : 0, nrd_m[T2S(GT_HOM_AA)]+nrd_mm[T2S(GT_HOM_AA)] ? nrd_mm[T2S(GT_HOM_AA)]*100.0/(nrd_m[T2S(GT_HOM_AA)]+nrd_mm[T2S(GT_HOM_AA)]) : 0 ); } for (x=0; x<2; x++) { gtcmp_t *stats; if ( x==0 ) { printf("# GCcS, Genotype concordance by sample (SNPs)\n# GCsS\t[2]id\t[3]sample\t[4]non-reference discordance rate\t[5]RR Hom matches\t[6]RA Het matches\t[7]AA Hom matches\t[8]RR Hom mismatches\t[9]RA Het mismatches\t[10]AA Hom mismatches\n"); stats = args->smpl_gts_snps; } else { printf("# GCiS, Genotype concordance by sample (indels)\n# GCiS\t[2]id\t[3]sample\t[4]non-reference discordance rate\t[5]RR Hom matches\t[6]RA Het matches\t[7]AA Hom matches\t[8]RR Hom mismatches\t[9]RA Het mismatches\t[10]AA Hom mismatches\n"); stats = args->smpl_gts_indels; } for (i=0; ifiles->n_smpl; i++) { uint64_t m = stats[i].m[T2S(GT_HET_RA)] + stats[i].m[T2S(GT_HOM_AA)]; uint64_t mm = stats[i].mm[T2S(GT_HOM_RR)] + stats[i].mm[T2S(GT_HET_RA)] + stats[i].mm[T2S(GT_HOM_AA)]; printf("GC%cS\t2\t%s\t%.3f", x==0 ? 's' : 'i', args->files->samples[i], m+mm ? mm*100.0/(m+mm) : 0); printf("\t%"PRId64"\t%"PRId64"\t%"PRId64"", stats[i].m[T2S(GT_HOM_RR)],stats[i].m[T2S(GT_HET_RA)],stats[i].m[T2S(GT_HOM_AA)]); printf("\t%"PRId64"\t%"PRId64"\t%"PRId64"\n", stats[i].mm[T2S(GT_HOM_RR)],stats[i].mm[T2S(GT_HET_RA)],stats[i].mm[T2S(GT_HOM_AA)]); } } } printf("# DP, Depth distribution\n# DP\t[2]id\t[3]bin\t[4]number of genotypes\t[5]fraction of genotypes (%%)\t[6]number of sites\t[7]fraction of sites (%%)\n"); for (id=0; idnstats; id++) { stats_t *stats = &args->stats[id]; long unsigned int sum = 0, sum_sites = 0; for (i=0; idp.m_vals; i++) { sum += stats->dp.vals[i]; sum_sites += stats->dp_sites.vals[i]; } for (i=0; idp.m_vals; i++) { if ( stats->dp.vals[i]==0 && stats->dp_sites.vals[i]==0 ) continue; printf("DP\t%d\t", id); if ( i==0 ) printf("<%d", stats->dp.min); else if ( i+1==stats->dp.m_vals ) printf(">%d", stats->dp.max); else printf("%d", idist_i2bin(&stats->dp,i)); printf("\t%"PRId64"\t%f", stats->dp.vals[i], sum ? stats->dp.vals[i]*100./sum : 0); printf("\t%"PRId64"\t%f\n", stats->dp_sites.vals[i], sum_sites ? stats->dp_sites.vals[i]*100./sum_sites : 0); } } if ( args->files->n_smpl ) { printf("# PSC, Per-sample counts\n# PSC\t[2]id\t[3]sample\t[4]nRefHom\t[5]nNonRefHom\t[6]nHets\t[7]nTransitions\t[8]nTransversions\t[9]nIndels\t[10]average depth\t[11]nSingletons\n"); for (id=0; idnstats; id++) { stats_t *stats = &args->stats[id]; for (i=0; ifiles->n_smpl; i++) { float dp = stats->smpl_ndp[i] ? stats->smpl_dp[i]/(float)stats->smpl_ndp[i] : 0; printf("PSC\t%d\t%s\t%d\t%d\t%d\t%d\t%d\t%d\t%.1f\t%d\n", id,args->files->samples[i], stats->smpl_homRR[i], stats->smpl_homAA[i], stats->smpl_hets[i], stats->smpl_ts[i], stats->smpl_tv[i], stats->smpl_indels[i],dp, stats->smpl_sngl[i]); } } if ( args->exons ) { printf("# PSI, Per-Sample Indels\n# PSI\t[2]id\t[3]sample\t[4]in-frame\t[5]out-frame\t[6]not applicable\t[7]out/(in+out) ratio\n"); for (id=0; idnstats; id++) { stats_t *stats = &args->stats[id]; for (i=0; ifiles->n_smpl; i++) { int na = stats->smpl_frm_shifts[i*3 + 0]; int in = stats->smpl_frm_shifts[i*3 + 1]; int out = stats->smpl_frm_shifts[i*3 + 2]; printf("PSI\t%d\t%s\t%d\t%d\t%d\t%.2f\n", id,args->files->samples[i], in,out,na,in+out?1.0*out/(in+out):0); } } } #ifdef HWE_STATS printf("# HWE\n# HWE\t[2]id\t[3]1st ALT allele frequency\t[4]Number of observations\t[5]25th percentile\t[6]median\t[7]75th percentile\n"); for (id=0; idnstats; id++) { stats_t *stats = &args->stats[id]; for (i=0; inaf_hwe; i++) stats->af_hwe[i+args->naf_hwe] += stats->af_hwe[i]; // singletons for (i=1; im_af; i++) { unsigned int sum_tot = 0, sum_tmp = 0; int j, *ptr = &stats->af_hwe[i*args->naf_hwe]; for (j=0; jnaf_hwe; j++) sum_tot += ptr[j]; if ( !sum_tot ) continue; int nprn = 3; printf("HWE\t%d\t%f\t%d",id,100.*(i-1)/(args->m_af-1),sum_tot); for (j=0; jnaf_hwe; j++) { sum_tmp += ptr[j]; float frac = (float)sum_tmp/sum_tot; if ( frac >= 0.75 ) { while (nprn>0) { printf("\t%f", (float)j/args->naf_hwe); nprn--; } break; } if ( frac >= 0.5 ) { while (nprn>1) { printf("\t%f", (float)j/args->naf_hwe); nprn--; } continue; } if ( frac >= 0.25 ) { while (nprn>2) { printf("\t%f", (float)j/args->naf_hwe); nprn--; } } } assert(nprn==0); printf("\n"); } } #endif } } static void usage(void) { fprintf(stderr, "\n"); fprintf(stderr, "About: Parses VCF or BCF and produces stats which can be plotted using plot-vcfstats.\n"); fprintf(stderr, " When two files are given, the program generates separate stats for intersection\n"); fprintf(stderr, " and the complements. By default only sites are compared, -s/-S must given to include\n"); fprintf(stderr, " also sample columns.\n"); fprintf(stderr, "Usage: bcftools stats [options] []\n"); fprintf(stderr, "\n"); fprintf(stderr, "Options:\n"); fprintf(stderr, " -1, --1st-allele-only include only 1st allele at multiallelic sites\n"); fprintf(stderr, " -c, --collapse treat as identical records with , see man page for details [none]\n"); fprintf(stderr, " -d, --depth depth distribution: min,max,bin size [0,500,1]\n"); fprintf(stderr, " -e, --exons tab-delimited file with exons for indel frameshifts (chr,from,to; 1-based, inclusive, bgzip compressed)\n"); fprintf(stderr, " -f, --apply-filters require at least one of the listed FILTER strings (e.g. \"PASS,.\")\n"); fprintf(stderr, " -F, --fasta-ref faidx indexed reference sequence file to determine INDEL context\n"); fprintf(stderr, " -i, --split-by-ID collect stats for sites with ID separately (known vs novel)\n"); fprintf(stderr, " -r, --regions restrict to comma-separated list of regions\n"); fprintf(stderr, " -R, --regions-file restrict to regions listed in a file\n"); fprintf(stderr, " -s, --samples list of samples for sample stats, \"-\" to include all samples\n"); fprintf(stderr, " -S, --samples-file file of samples to include\n"); fprintf(stderr, " -t, --targets similar to -r but streams rather than index-jumps\n"); fprintf(stderr, " -T, --targets-file similar to -R but streams rather than index-jumps\n"); fprintf(stderr, " -u, --user-tstv collect Ts/Tv stats for any tag using the given binning [0:1:100]\n"); fprintf(stderr, " -v, --verbose produce verbose per-site and per-sample output\n"); fprintf(stderr, "\n"); exit(1); } int main_vcfstats(int argc, char *argv[]) { int c; args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->files = bcf_sr_init(); args->argc = argc; args->argv = argv; args->dp_min = 0; args->dp_max = 500; args->dp_step = 1; int regions_is_file = 0, targets_is_file = 0; static struct option loptions[] = { {"1st-allele-only",0,0,'1'}, {"help",0,0,'h'}, {"collapse",1,0,'c'}, {"regions",1,0,'r'}, {"regions-file",1,0,'R'}, {"verbose",0,0,'v'}, {"depth",1,0,'d'}, {"apply-filters",1,0,'f'}, {"exons",1,0,'e'}, {"samples",1,0,'s'}, {"samples-file",1,0,'S'}, {"split-by-ID",0,0,'i'}, {"targets",1,0,'t'}, {"targets-file",1,0,'T'}, {"fasta-ref",1,0,'F'}, {"user-tstv",1,0,'u'}, {0,0,0,0} }; while ((c = getopt_long(argc, argv, "hc:r:R:e:s:S:d:it:T:F:f:1u:v",loptions,NULL)) >= 0) { switch (c) { case 'u': add_user_stats(args,optarg); break; case '1': args->first_allele_only = 1; break; case 'F': args->ref_fname = optarg; break; case 't': args->targets_list = optarg; break; case 'T': args->targets_list = optarg; targets_is_file = 1; break; case 'c': if ( !strcmp(optarg,"snps") ) args->files->collapse |= COLLAPSE_SNPS; else if ( !strcmp(optarg,"indels") ) args->files->collapse |= COLLAPSE_INDELS; else if ( !strcmp(optarg,"both") ) args->files->collapse |= COLLAPSE_SNPS | COLLAPSE_INDELS; else if ( !strcmp(optarg,"any") ) args->files->collapse |= COLLAPSE_ANY; else if ( !strcmp(optarg,"all") ) args->files->collapse |= COLLAPSE_ANY; else if ( !strcmp(optarg,"some") ) args->files->collapse |= COLLAPSE_SOME; else if ( !strcmp(optarg,"none") ) args->files->collapse = COLLAPSE_NONE; else error("The --collapse string \"%s\" not recognised.\n", optarg); break; case 'v': args->verbose_sites = 1; break; case 'd': if ( sscanf(optarg,"%d,%d,%d",&args->dp_min,&args->dp_max,&args->dp_step)!=3 ) error("Could not parse --depth %s\n", optarg); if ( args->dp_min<0 || args->dp_min >= args->dp_max || args->dp_step > args->dp_max - args->dp_min + 1 ) error("Is this a typo? --depth %s\n", optarg); break; case 'f': args->files->apply_filters = optarg; break; case 'r': args->regions_list = optarg; break; case 'R': args->regions_list = optarg; regions_is_file = 1; break; case 'e': args->exons_fname = optarg; break; case 's': args->samples_list = optarg; break; case 'S': args->samples_list = optarg; args->samples_is_file = 1; break; case 'i': args->split_by_id = 1; break; case 'h': case '?': usage(); default: error("Unknown argument: %s\n", optarg); } } char *fname = NULL; if ( optind==argc ) { if ( !isatty(fileno((FILE *)stdin)) ) fname = "-"; // reading from stdin else usage(); } else fname = argv[optind]; if ( argc-optind>2 ) usage(); if ( argc-optind>1 ) { args->files->require_index = 1; if ( args->split_by_id ) error("Only one file can be given with -i.\n"); } if ( !args->samples_list ) args->files->max_unpack = BCF_UN_INFO; if ( args->targets_list && bcf_sr_set_targets(args->files, args->targets_list, targets_is_file, 0)<0 ) error("Failed to read the targets: %s\n", args->targets_list); if ( args->regions_list && bcf_sr_set_regions(args->files, args->regions_list, regions_is_file)<0 ) error("Failed to read the regions: %s\n", args->regions_list); while (fname) { if ( !bcf_sr_add_reader(args->files, fname) ) error("Failed to open %s: %s\n", fname,bcf_sr_strerror(args->files->errnum)); fname = ++optind < argc ? argv[optind] : NULL; } init_stats(args); print_header(args); do_vcf_stats(args); print_stats(args); destroy_stats(args); bcf_sr_destroy(args->files); free(args); return 0; } bcftools-1.2/vcfutils.pl000077500000000000000000000434271246371514100154040ustar00rootroot00000000000000#!/usr/bin/perl -w # # Copyright (C) 2010 Broad Institute. # Copyright (C) 2011, 2014 Genome Research Ltd. # # Author: Heng Li # # Permission is hereby granted, free of charge, to any person obtaining a copy # of this software and associated documentation files (the "Software"), to deal # in the Software without restriction, including without limitation the rights # to use, copy, modify, merge, publish, distribute, sublicense, and/or sell # copies of the Software, and to permit persons to whom the Software is # furnished to do so, subject to the following conditions: # # The above copyright notice and this permission notice shall be included in # all copies or substantial portions of the Software. # # THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR # IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, # FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL # THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER # LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING # FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER # DEALINGS IN THE SOFTWARE. use strict; use warnings; use Getopt::Std; &main; exit; sub main { &usage if (@ARGV < 1); my $command = shift(@ARGV); my %func = (subsam=>\&subsam, listsam=>\&listsam, fillac=>\&fillac, qstats=>\&qstats, varFilter=>\&varFilter, hapmap2vcf=>\&hapmap2vcf, ucscsnp2vcf=>\&ucscsnp2vcf, filter4vcf=>\&varFilter, ldstats=>\&ldstats, gapstats=>\&gapstats, splitchr=>\&splitchr, vcf2fq=>\&vcf2fq); die("Unknown command \"$command\".\n") if (!defined($func{$command})); &{$func{$command}}; } sub splitchr { my %opts = (l=>5000000); getopts('l:', \%opts); my $l = $opts{l}; die(qq/Usage: vcfutils.pl splitchr [-l $opts{l}] \n/) if (@ARGV == 0 && -t STDIN); while (<>) { my @t = split; my $last = 0; for (my $i = 0; $i < $t[1];) { my $e = ($t[1] - $i) / $l < 1.1? $t[1] : $i + $l; print "$t[0]:".($i+1)."-$e\n"; $i = $e; } } } sub subsam { die(qq/Usage: vcfutils.pl subsam [samples]\n/) if (@ARGV == 0); my ($fh, %h); my $fn = shift(@ARGV); my @col; open($fh, ($fn =~ /\.gz$/)? "gzip -dc $fn |" : $fn) || die; $h{$_} = 1 for (@ARGV); while (<$fh>) { if (/^##/) { print; } elsif (/^#/) { my @t = split; my @s = @t[0..8]; # all fixed fields + FORMAT for (9 .. $#t) { if ($h{$t[$_]}) { push(@s, $t[$_]); push(@col, $_); } } pop(@s) if (@s == 9); # no sample selected; remove the FORMAT field print join("\t", @s), "\n"; } else { my @t = split; if (@col == 0) { print join("\t", @t[0..7]), "\n"; } else { print join("\t", @t[0..8], map {$t[$_]} @col), "\n"; } } } close($fh); } sub listsam { die(qq/Usage: vcfutils.pl listsam \n/) if (@ARGV == 0 && -t STDIN); while (<>) { if (/^#/ && !/^##/) { my @t = split; print join("\n", @t[9..$#t]), "\n"; exit; } } } sub fillac { die(qq/Usage: vcfutils.pl fillac \n\nNote: The GT field MUST BE present and always appear as the first field.\n/) if (@ARGV == 0 && -t STDIN); while (<>) { if (/^#/) { print; } else { my @t = split; my @c = (0, 0); my $n = 0; my $s = -1; @_ = split(":", $t[8]); for (0 .. $#_) { if ($_[$_] eq 'GT') { $s = $_; last; } } if ($s < 0) { print join("\t", @t), "\n"; next; } for (9 .. $#t) { if ($t[$_] =~ /^0,0,0/) { } elsif ($t[$_] =~ /^([^\s:]+:){$s}(\d+).(\d+)/) { ++$c[$2]; ++$c[$3]; $n += 2; } } my $AC = "AC=" . join("\t", @c[1..$#c]) . ";AN=$n"; my $info = $t[7]; $info =~ s/(;?)AC=(\d+)//; $info =~ s/(;?)AN=(\d+)//; if ($info eq '.') { $info = $AC; } else { $info .= ";$AC"; } $t[7] = $info; print join("\t", @t), "\n"; } } } sub ldstats { my %opts = (t=>0.9); getopts('t:', \%opts); die("Usage: vcfutils.pl ldstats [-t $opts{t}] \n") if (@ARGV == 0 && -t STDIN); my $cutoff = $opts{t}; my ($last, $lastchr) = (0x7fffffff, ''); my ($x, $y, $n) = (0, 0, 0); while (<>) { if (/^([^#\s]+)\s(\d+)/) { my ($chr, $pos) = ($1, $2); if (/NEIR=([\d\.]+)/) { ++$n; ++$y, $x += $pos - $last if ($lastchr eq $chr && $pos > $last && $1 > $cutoff); } $last = $pos; $lastchr = $chr; } } print "Number of SNP intervals in strong LD (r > $opts{t}): $y\n"; print "Fraction: ", $y/$n, "\n"; print "Length: $x\n"; } sub qstats { my %opts = (r=>'', s=>0.02, v=>undef); getopts('r:s:v', \%opts); die("Usage: vcfutils.pl qstats [-r ref.vcf] \n Note: This command discards indels. Output: QUAL #non-indel #SNPs #transitions #joint ts/tv #joint/#ref #joint/#non-indel \n") if (@ARGV == 0 && -t STDIN); my %ts = (AG=>1, GA=>1, CT=>1, TC=>1); my %h = (); my $is_vcf = defined($opts{v})? 1 : 0; if ($opts{r}) { # read the reference positions my $fh; open($fh, $opts{r}) || die; while (<$fh>) { next if (/^#/); if ($is_vcf) { my @t = split; $h{$t[0],$t[1]} = $t[4]; } else { $h{$1,$2} = 1 if (/^(\S+)\s+(\d+)/); } } close($fh); } my $hsize = scalar(keys %h); my @a; while (<>) { next if (/^#/); my @t = split; next if (length($t[3]) != 1 || uc($t[3]) eq 'N'); $t[3] = uc($t[3]); $t[4] = uc($t[4]); my @s = split(',', $t[4]); $t[5] = 3 if ($t[5] eq '.' || $t[5] < 0); next if (length($s[0]) != 1); my $hit; if ($is_vcf) { $hit = 0; my $aa = $h{$t[0],$t[1]}; if (defined($aa)) { my @aaa = split(",", $aa); for (@aaa) { $hit = 1 if ($_ eq $s[0]); } } } else { $hit = defined($h{$t[0],$t[1]})? 1 : 0; } push(@a, [$t[5], ($t[4] eq '.' || $t[4] eq $t[3])? 0 : 1, $ts{$t[3].$s[0]}? 1 : 0, $hit]); } push(@a, [-1, 0, 0, 0]); # end marker die("[qstats] No SNP data!\n") if (@a == 0); @a = sort {$b->[0]<=>$a->[0]} @a; my $next = $opts{s}; my $last = $a[0]; my @c = (0, 0, 0, 0); my @lc; $lc[1] = $lc[2] = 0; for my $p (@a) { if ($p->[0] == -1 || ($p->[0] != $last && $c[0]/@a > $next)) { my @x; $x[0] = sprintf("%.4f", $c[1]-$c[2]? $c[2] / ($c[1] - $c[2]) : 100); $x[1] = sprintf("%.4f", $hsize? $c[3] / $hsize : 0); $x[2] = sprintf("%.4f", $c[3] / $c[1]); my $a = $c[1] - $lc[1]; my $b = $c[2] - $lc[2]; $x[3] = sprintf("%.4f", $a-$b? $b / ($a-$b) : 100); print join("\t", $last, @c, @x), "\n"; $next = $c[0]/@a + $opts{s}; $lc[1] = $c[1]; $lc[2] = $c[2]; } ++$c[0]; $c[1] += $p->[1]; $c[2] += $p->[2]; $c[3] += $p->[3]; $last = $p->[0]; } } sub varFilter { my %opts = (d=>2, D=>10000000, a=>2, W=>10, Q=>10, w=>3, p=>undef, 1=>1e-4, 2=>1e-100, 3=>0, 4=>1e-4, G=>0, S=>1000, e=>1e-4); getopts('pd:D:W:Q:w:a:1:2:3:4:G:S:e:', \%opts); die(qq/ Usage: vcfutils.pl varFilter [options] Options: -Q INT minimum RMS mapping quality for SNPs [$opts{Q}] -d INT minimum read depth [$opts{d}] -D INT maximum read depth [$opts{D}] -a INT minimum number of alternate bases [$opts{a}] -w INT SNP within INT bp around a gap to be filtered [$opts{w}] -W INT window size for filtering adjacent gaps [$opts{W}] -1 FLOAT min P-value for strand bias (given PV4) [$opts{1}] -2 FLOAT min P-value for baseQ bias [$opts{2}] -3 FLOAT min P-value for mapQ bias [$opts{3}] -4 FLOAT min P-value for end distance bias [$opts{4}] -e FLOAT min P-value for HWE (plus F<0) [$opts{e}] -p print filtered variants Note: Some of the filters rely on annotations generated by SAMtools\/BCFtools. \n/) if (@ARGV == 0 && -t STDIN); # calculate the window size my ($ol, $ow) = ($opts{W}, $opts{w}); my $max_dist = $ol > $ow? $ol : $ow; # the core loop my @staging; # (indel_filtering_score, flt_tag, indel_span; chr, pos, ...) while (<>) { my @t = split; if (/^#/) { print; next; } next if ($t[4] eq '.'); # skip non-var sites next if ($t[3] eq 'N'); # skip sites with unknown ref ('N') # check if the site is a SNP my $type = 1; # SNP if (length($t[3]) > 1) { $type = 2; # MNP my @s = split(',', $t[4]); for (@s) { $type = 3 if (length != length($t[3])); } } else { my @s = split(',', $t[4]); for (@s) { $type = 3 if (length > 1); } } # clear the out-of-range elements while (@staging) { # Still on the same chromosome and the first element's window still affects this position? last if ($staging[0][3] eq $t[0] && $staging[0][4] + $staging[0][2] + $max_dist >= $t[1]); varFilter_aux(shift(@staging), $opts{p}); # calling a function is a bit slower, not much } my $flt = 0; # parse annotations my ($dp, $mq, $dp_alt) = (-1, -1, -1); if ($t[7] =~ /DP4=(\d+),(\d+),(\d+),(\d+)/i) { $dp = $1 + $2 + $3 + $4; $dp_alt = $3 + $4; } if ($t[7] =~ /DP=(\d+)/i) { $dp = $1; } $mq = $1 if ($t[7] =~ /MQ=(\d+)/i); # the depth and mapQ filter if ($dp >= 0) { if ($dp < $opts{d}) { $flt = 2; } elsif ($dp > $opts{D}) { $flt = 3; } } $flt = 4 if ($dp_alt >= 0 && $dp_alt < $opts{a}); $flt = 1 if ($flt == 0 && $mq >= 0 && $mq < $opts{Q}); $flt = 7 if ($flt == 0 && /PV4=([^,]+),([^,]+),([^,]+),([^,;\t]+)/ && ($1<$opts{1} || $2<$opts{2} || $3<$opts{3} || $4<$opts{4})); $flt = 8 if ($flt == 0 && ((/MXGQ=(\d+)/ && $1 < $opts{G}) || (/MXSP=(\d+)/ && $1 >= $opts{S}))); # HWE filter if ($t[7] =~ /G3=([^;,]+),([^;,]+),([^;,]+).*HWE=([^;,]+)/ && $4 < $opts{e}) { my $p = 2*$1 + $2; my $f = ($p > 0 && $p < 1)? 1 - $2 / ($p * (1-$p)) : 0; $flt = 9 if ($f < 0); } my $score = $t[5] * 100 + $dp_alt; my $rlen = length($t[3]) - 1; # $indel_score<0 for SNPs if ($flt == 0) { if ($type == 3) { # an indel # filtering SNPs and MNPs for my $x (@staging) { next if (($x->[0]&3) == 3 || $x->[1] || $x->[4] + $x->[2] + $ow < $t[1]); $x->[1] = 5; } # check the staging list for indel filtering for my $x (@staging) { next if (($x->[0]&3) != 3 || $x->[1] || $x->[4] + $x->[2] + $ol < $t[1]); if ($x->[0]>>2 < $score) { $x->[1] = 6; } else { $flt = 6; last; } } } else { # SNP or MNP for my $x (@staging) { next if (($x->[0]&3) != 3 || $x->[4] + $x->[2] + $ow < $t[1]); if ($x->[4] + length($x->[7]) - 1 == $t[1] && substr($x->[7], -1, 1) eq substr($t[4], 0, 1) && length($x->[7]) - length($x->[6]) == 1) { $x->[1] = 5; } else { $flt = 5; } last; } # check MNP for my $x (@staging) { next if (($x->[0]&3) == 3 || $x->[4] + $x->[2] < $t[1]); if ($x->[0]>>2 < $score) { $x->[1] = 8; } else { $flt = 8; last; } } } } push(@staging, [$score<<2|$type, $flt, $rlen, @t]); } # output the last few elements in the staging list while (@staging) { varFilter_aux(shift @staging, $opts{p}); } } sub varFilter_aux { my ($first, $is_print) = @_; if ($first->[1] == 0) { print join("\t", @$first[3 .. @$first-1]), "\n"; } elsif ($is_print) { print STDERR join("\t", substr("UQdDaGgPMS", $first->[1], 1), @$first[3 .. @$first-1]), "\n"; } } sub gapstats { my (@c0, @c1); $c0[$_] = $c1[$_] = 0 for (0 .. 10000); while (<>) { next if (/^#/); my @t = split; next if (length($t[3]) == 1 && $t[4] =~ /^[A-Za-z](,[A-Za-z])*$/); # not an indel my @s = split(',', $t[4]); for my $x (@s) { my $l = length($x) - length($t[3]) + 5000; if ($x =~ /^-/) { $l = -(length($x) - 1) + 5000; } elsif ($x =~ /^\+/) { $l = length($x) - 1 + 5000; } $c0[$l] += 1 / @s; } } for (my $i = 0; $i < 10000; ++$i) { next if ($c0[$i] == 0); $c1[0] += $c0[$i]; $c1[1] += $c0[$i] if (($i-5000)%3 == 0); printf("C\t%d\t%.2f\n", ($i-5000), $c0[$i]); } printf("3\t%d\t%d\t%.3f\n", $c1[0], $c1[1], $c1[1]/$c1[0]); } sub ucscsnp2vcf { die("Usage: vcfutils.pl \n") if (@ARGV == 0 && -t STDIN); print "##fileformat=VCFv4.0\n"; print join("\t", "#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO"), "\n"; while (<>) { my @t = split("\t"); my $indel = ($t[9] =~ /^[ACGT](\/[ACGT])+$/)? 0 : 1; my $pos = $t[2] + 1; my @alt; push(@alt, $t[7]); if ($t[6] eq '-') { $t[9] = reverse($t[9]); $t[9] =~ tr/ACGTRYMKWSNacgtrymkwsn/TGCAYRKMWSNtgcayrkmwsn/; } my @a = split("/", $t[9]); for (@a) { push(@alt, $_) if ($_ ne $alt[0]); } if ($indel) { --$pos; for (0 .. $#alt) { $alt[$_] =~ tr/-//d; $alt[$_] = "N$alt[$_]"; } } my $ref = shift(@alt); my $af = $t[13] > 0? ";AF=$t[13]" : ''; my $valid = ($t[12] eq 'unknown')? '' : ";valid=$t[12]"; my $info = "molType=$t[10];class=$t[11]$valid$af"; print join("\t", $t[1], $pos, $t[4], $ref, join(",", @alt), 0, '.', $info), "\n"; } } sub hapmap2vcf { die("Usage: vcfutils.pl \n") if (@ARGV == 0); my $fn = shift(@ARGV); # parse UCSC SNP warn("Parsing UCSC SNPs...\n"); my ($fh, %map); open($fh, ($fn =~ /\.gz$/)? "gzip -dc $fn |" : $fn) || die; while (<$fh>) { my @t = split; next if ($t[3] - $t[2] != 1); # not SNP @{$map{$t[4]}} = @t[1,3,7]; } close($fh); # write VCF warn("Writing VCF...\n"); print "##fileformat=VCFv4.0\n"; while (<>) { my @t = split; if ($t[0] eq 'rs#') { # the first line print join("\t", "#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO\tFORMAT", @t[11..$#t]), "\n"; } else { next unless ($map{$t[0]}); next if (length($t[1]) != 3); # skip non-SNPs my $a = \@{$map{$t[0]}}; my $ref = $a->[2]; my @u = split('/', $t[1]); if ($u[1] eq $ref) { $u[1] = $u[0]; $u[0] = $ref; } elsif ($u[0] ne $ref) { next; } my $alt = $u[1]; my %w; $w{$u[0]} = 0; $w{$u[1]} = 1; my @s = (@$a[0,1], $t[0], $ref, $alt, 0, '.', '.', 'GT'); my $is_tri = 0; for (@t[11..$#t]) { if ($_ eq 'NN') { push(@s, './.'); } else { my @a = ($w{substr($_,0,1)}, $w{substr($_,1,1)}); if (!defined($a[0]) || !defined($a[1])) { $is_tri = 1; last; } push(@s, "$a[0]/$a[1]"); } } next if ($is_tri); print join("\t", @s), "\n"; } } } sub vcf2fq { my %opts = (d=>3, D=>100000, Q=>10, l=>5); getopts('d:D:Q:l:', \%opts); die(qq/ Usage: vcfutils.pl vcf2fq [options] Options: -d INT minimum depth [$opts{d}] -D INT maximum depth [$opts{D}] -Q INT min RMS mapQ [$opts{Q}] -l INT INDEL filtering window [$opts{l}] \n/) if (@ARGV == 0 && -t STDIN); my ($last_chr, $seq, $qual, $last_pos, @gaps); my $_Q = $opts{Q}; my $_d = $opts{d}; my $_D = $opts{D}; my %het = (AC=>'M', AG=>'R', AT=>'W', CA=>'M', CG=>'S', CT=>'Y', GA=>'R', GC=>'S', GT=>'K', TA=>'W', TC=>'Y', TG=>'K'); $last_chr = ''; while (<>) { next if (/^#/); my @t = split; if ($last_chr ne $t[0]) { &v2q_post_process($last_chr, \$seq, \$qual, \@gaps, $opts{l}) if ($last_chr); ($last_chr, $last_pos) = ($t[0], 0); $seq = $qual = ''; @gaps = (); } die("[vcf2fq] unsorted input\n") if ($t[1] - $last_pos < 0); if ($t[1] - $last_pos > 1) { $seq .= 'n' x ($t[1] - $last_pos - 1); $qual .= '!' x ($t[1] - $last_pos - 1); } if (length($t[3]) == 1 && $t[7] !~ /INDEL/ && $t[4] =~ /^([A-Za-z.])(,[A-Za-z])*$/) { # a SNP or reference my ($ref, $alt) = ($t[3], $1); my ($b, $q); $q = $1 if ($t[7] =~ /FQ=(-?[\d\.]+)/); if ($q < 0) { $_ = ($t[7] =~ /AF1=([\d\.]+)/)? $1 : 0; $b = ($_ < .5 || $alt eq '.')? $ref : $alt; $q = -$q; } else { $b = $het{"$ref$alt"}; $b ||= 'N'; } $b = lc($b); $b = uc($b) if (($t[7] =~ /MQ=(\d+)/ && $1 >= $_Q) && ($t[7] =~ /DP=(\d+)/ && $1 >= $_d && $1 <= $_D)); $q = int($q + 33 + .499); $q = chr($q <= 126? $q : 126); $seq .= $b; $qual .= $q; } elsif ($t[4] ne '.') { # an INDEL push(@gaps, [$t[1], length($t[3])]); } $last_pos = $t[1]; } &v2q_post_process($last_chr, \$seq, \$qual, \@gaps, $opts{l}); } sub v2q_post_process { my ($chr, $seq, $qual, $gaps, $l) = @_; for my $g (@$gaps) { my $beg = $g->[0] > $l? $g->[0] - $l : 0; my $end = $g->[0] + $g->[1] + $l; $end = length($$seq) if ($end > length($$seq)); substr($$seq, $beg, $end - $beg) = lc(substr($$seq, $beg, $end - $beg)); } print "\@$chr\n"; &v2q_print_str($seq); print "+\n"; &v2q_print_str($qual); } sub v2q_print_str { my ($s) = @_; my $l = length($$s); for (my $i = 0; $i < $l; $i += 60) { print substr($$s, $i, 60), "\n"; } } sub usage { die(qq/ Usage: vcfutils.pl []\n Command: subsam get a subset of samples listsam list the samples fillac fill the allele count field qstats SNP stats stratified by QUAL hapmap2vcf convert the hapmap format to VCF ucscsnp2vcf convert UCSC SNP SQL dump to VCF varFilter filtering short variants (*) vcf2fq VCF->fastq (**) Notes: Commands with description endting with (*) may need bcftools specific annotations. \n/); } bcftools-1.2/vcfview.c000066400000000000000000001011311246371514100150050ustar00rootroot00000000000000/* vcfview.c -- VCF/BCF conversion, view, subset and filter VCF/BCF files. Copyright (C) 2013-2014 Genome Research Ltd. Author: Shane McCarthy Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include #include #include #include "bcftools.h" #include "filter.h" #include "htslib/khash_str2int.h" #define FLT_INCLUDE 1 #define FLT_EXCLUDE 2 #define ALLELE_NONREF 1 #define ALLELE_MINOR 2 #define ALLELE_ALT1 3 #define ALLELE_MAJOR 4 #define ALLELE_NONMAJOR 5 #define GT_NEED_HOM 1 #define GT_NEED_HET 2 #define GT_NO_HOM 3 #define GT_NO_HET 4 #define GT_NEED_MISSING 5 #define GT_NO_MISSING 6 typedef struct _args_t { filter_t *filter; char *filter_str; int filter_logic; // one of FLT_INCLUDE/FLT_EXCLUDE (-i or -e) bcf_srs_t *files; bcf_hdr_t *hdr, *hnull, *hsub; // original header, sites-only header, subset header char **argv, *format, *sample_names, *subset_fname, *targets_list, *regions_list; int argc, clevel, output_type, print_header, update_info, header_only, n_samples, *imap, calc_ac; int trim_alts, sites_only, known, novel, min_alleles, max_alleles, private_vars, uncalled, phased; int min_ac, min_ac_type, max_ac, max_ac_type, min_af_type, max_af_type, gt_type; int *ac, mac; float min_af, max_af; char *fn_ref, *fn_out, **samples; int sample_is_file, force_samples; char *include_types, *exclude_types; int include, exclude; htsFile *out; } args_t; static void init_data(args_t *args) { int i; args->hdr = args->files->readers[0].header; if (args->calc_ac && args->update_info) { bcf_hdr_append(args->hdr,"##INFO="); bcf_hdr_append(args->hdr,"##INFO="); } bcf_hdr_append_version(args->hdr, args->argc, args->argv, "bcftools_view"); // setup sample data if (args->sample_names) { void *hdr_samples = khash_str2int_init(); for (i=0; ihdr); i++) khash_str2int_inc(hdr_samples, bcf_hdr_int2id(args->hdr,BCF_DT_SAMPLE,i)); void *exclude = (args->sample_names[0]=='^') ? khash_str2int_init() : NULL; int nsmpl; char **smpl = NULL; args->samples = NULL; args->n_samples = 0; smpl = hts_readlist(exclude ? &args->sample_names[1] : args->sample_names, args->sample_is_file, &nsmpl); if ( !smpl ) { error("Could not read the list: \"%s\"\n", exclude ? &args->sample_names[1] : args->sample_names); } if ( exclude ) { for (i=0; iforce_samples) { fprintf(stderr, "Warn: exclude called for sample that does not exist in header: \"%s\"... skipping\n", smpl[i]); } else { error("Error: exclude called for sample that does not exist in header: \"%s\". Use \"--force-samples\" to ignore this error.\n", smpl[i]); } } khash_str2int_inc(exclude, smpl[i]); } for (i=0; ihdr); i++) { if ( exclude && khash_str2int_has_key(exclude,bcf_hdr_int2id(args->hdr,BCF_DT_SAMPLE,i)) ) continue; args->samples = (char**) realloc(args->samples, (args->n_samples+1)*sizeof(const char*)); args->samples[args->n_samples++] = strdup(bcf_hdr_int2id(args->hdr,BCF_DT_SAMPLE,i)); } khash_str2int_destroy(exclude); } else { for (i=0; iforce_samples) { fprintf(stderr, "Warn: subset called for sample that does not exist in header: \"%s\"... skipping\n", smpl[i]); continue; } else { error("Error: subset called for sample that does not exist in header: \"%s\". Use \"--force-samples\" to ignore this error.\n", smpl[i]); } } args->samples = (char**) realloc(args->samples, (args->n_samples+1)*sizeof(const char*)); args->samples[args->n_samples++] = strdup(smpl[i]); } } for (i=0; in_samples == 0) { fprintf(stderr, "Warn: subsetting has removed all samples\n"); args->sites_only = 1; } } if (args->n_samples) args->imap = (int*)malloc(args->n_samples * sizeof(int)); // determine variant types to include/exclude if (args->include_types || args->exclude_types) { if (args->include_types && args->exclude_types) { fprintf(stderr, "Error: only supply one of --include-types, --exclude-types options\n"); exit(1); } char **type_list = 0; int m = 0, n = 0; const char *q, *p; for (q = p = args->include_types ? args->include_types : args->exclude_types;; ++p) { if (*p == ',' || *p == 0) { if (m == n) { m = m? m<<1 : 16; type_list = (char**)realloc(type_list, m * sizeof(char*)); } type_list[n] = (char*)calloc(p - q + 1, 1); strncpy(type_list[n++], q, p - q); q = p + 1; if (*p == 0) break; } } type_list = (char**)realloc(type_list, n * sizeof(char*)); if (args->include_types) { args->include = 0; for (i = 0; i < n; ++i) { if (strcmp(type_list[i], "snps") == 0) args->include |= VCF_SNP; else if (strcmp(type_list[i], "indels") == 0) args->include |= VCF_INDEL; else if (strcmp(type_list[i], "mnps") == 0) args->include |= VCF_MNP; else if (strcmp(type_list[i], "other") == 0) args->include |= VCF_OTHER; else { fprintf(stderr, "[E::%s] unknown type\n", type_list[i]); exit(1); } } } if (args->exclude_types) { args->exclude = 0; for (i = 0; i < n; ++i) { if (strcmp(type_list[i], "snps") == 0) args->exclude |= VCF_SNP; else if (strcmp(type_list[i], "indels") == 0) args->exclude |= VCF_INDEL; else if (strcmp(type_list[i], "mnps") == 0) args->exclude |= VCF_MNP; else if (strcmp(type_list[i], "other") == 0) args->exclude |= VCF_OTHER; else { fprintf(stderr, "[E::%s] unknown type\n", type_list[i]); exit(1); } } } for (i = 0; i < n; ++i) free(type_list[i]); free(type_list); } // setup output char modew[8]; strcpy(modew, "w"); if (args->clevel >= 0 && args->clevel <= 9) sprintf(modew + 1, "%d", args->clevel); if (args->output_type==FT_BCF) strcat(modew, "bu"); // uncompressed BCF else if (args->output_type & FT_BCF) strcat(modew, "b"); // compressed BCF else if (args->output_type & FT_GZ) strcat(modew,"z"); // compressed VCF args->out = hts_open(args->fn_out ? args->fn_out : "-", modew); if ( !args->out ) error("%s: %s\n", args->fn_out,strerror(errno)); // headers: hdr=full header, hsub=subset header, hnull=sites only header if (args->sites_only) args->hnull = bcf_hdr_subset(args->hdr, 0, 0, 0); if (args->n_samples > 0) { args->hsub = bcf_hdr_subset(args->hdr, args->n_samples, args->samples, args->imap); if ( !args->hsub ) error("Error occurred while subsetting samples\n"); if ( args->n_samples != bcf_hdr_nsamples(args->hsub) ) { int i; for (i=0; in_samples; i++) if ( args->imap[i]<0 ) error("Error: No such sample: \"%s\"\n", args->samples[i]); } } if ( args->filter_str ) args->filter = filter_init(args->hdr, args->filter_str); } static void destroy_data(args_t *args) { int i; if ( args->imap ) { for (i = 0; i < args->n_samples; ++i) free(args->samples[i]); free(args->samples); free(args->imap); } if (args->hnull) bcf_hdr_destroy(args->hnull); if (args->hsub) bcf_hdr_destroy(args->hsub); if ( args->filter ) filter_destroy(args->filter); free(args->ac); } // true if all samples are phased. // haploid genotypes are considered phased // ./. => not phased, .|. => phased int bcf_all_phased(const bcf_hdr_t *header, bcf1_t *line) { bcf_unpack(line, BCF_UN_FMT); bcf_fmt_t *fmt_ptr = bcf_get_fmt(header, line, "GT"); int all_phased = 1; if ( fmt_ptr ) { int i, isample; for (isample=0; isamplen_sample; isample++) { int sample_phased = 0; #define BRANCH_INT(type_t,vector_end) { \ type_t *p = (type_t*) (fmt_ptr->p + isample*fmt_ptr->size); \ for (i=0; in; i++) \ { \ if (fmt_ptr->n == 1 || (p[i] == vector_end && i == 1)) { sample_phased = 1; break; } /* haploid phased by definition */ \ if ( p[i] == vector_end ) { break; }; /* smaller ploidy */ \ if ( bcf_gt_is_missing(p[i]) ) continue; /* missing allele */ \ if ((p[i])&1) { \ sample_phased = 1; \ break; \ } \ } \ } switch (fmt_ptr->type) { case BCF_BT_INT8: BRANCH_INT(int8_t, bcf_int8_vector_end); break; case BCF_BT_INT16: BRANCH_INT(int16_t, bcf_int16_vector_end); break; case BCF_BT_INT32: BRANCH_INT(int32_t, bcf_int32_vector_end); break; default: fprintf(stderr, "[E::%s] todo: fmt_type %d\n", __func__, fmt_ptr->type); exit(1); break; } #undef BRANCH_INT if (!sample_phased) { all_phased = 0; break; } } } return all_phased; } int subset_vcf(args_t *args, bcf1_t *line) { if ( args->min_alleles && line->n_allele < args->min_alleles ) return 0; // min alleles if ( args->max_alleles && line->n_allele > args->max_alleles ) return 0; // max alleles if (args->novel || args->known) { if ( args->novel && (line->d.id[0]!='.' || line->d.id[1]!=0) ) return 0; // skip sites which are known, ID != '.' if ( args->known && line->d.id[0]=='.' && line->d.id[1]==0 ) return 0; // skip sites which are novel, ID == '.' } if (args->include || args->exclude) { int line_type = bcf_get_variant_types(line); if ( args->include && !(line_type&args->include) ) return 0; // include only given variant types if ( args->exclude && line_type&args->exclude ) return 0; // exclude given variant types } if ( args->filter ) { int ret = filter_test(args->filter, line, NULL); if ( args->filter_logic==FLT_INCLUDE ) { if ( !ret ) return 0; } else if ( ret ) return 0; } hts_expand(int, line->n_allele, args->mac, args->ac); int i, an = 0, non_ref_ac = 0; if (args->calc_ac) { bcf_calc_ac(args->hdr, line, args->ac, BCF_UN_INFO|BCF_UN_FMT); // get original AC and AN values from INFO field if available, otherwise calculate for (i=1; in_allele; i++) non_ref_ac += args->ac[i]; for (i=0; in_allele; i++) an += args->ac[i]; } if (args->n_samples) { int non_ref_ac_sub = 0, *ac_sub = (int*) calloc(line->n_allele,sizeof(int)); bcf_subset(args->hdr, line, args->n_samples, args->imap); if (args->calc_ac) { bcf_calc_ac(args->hsub, line, ac_sub, BCF_UN_FMT); // recalculate AC and AN an = 0; for (i=0; in_allele; i++) { args->ac[i] = ac_sub[i]; an += ac_sub[i]; } for (i=1; in_allele; i++) non_ref_ac_sub += ac_sub[i]; if (args->private_vars) { if (args->private_vars == FLT_INCLUDE && !(non_ref_ac_sub > 0 && non_ref_ac == non_ref_ac_sub)) { free(ac_sub); return 0; } // select private sites if (args->private_vars == FLT_EXCLUDE && non_ref_ac_sub > 0 && non_ref_ac == non_ref_ac_sub) { free(ac_sub); return 0; } // exclude private sites } non_ref_ac = non_ref_ac_sub; } free(ac_sub); } bcf_fmt_t *gt_fmt; if ( args->gt_type && (gt_fmt=bcf_get_fmt(args->hdr,line,"GT")) ) { int nhet = 0, nhom = 0, nmiss = 0; for (i=0; ihdr); i++) { int type = bcf_gt_type(gt_fmt,i,NULL,NULL); if ( type==GT_HET_RA || type==GT_HET_AA ) { if ( args->gt_type==GT_NO_HET ) return 0; nhet = 1; } else if ( type==GT_UNKN ) { if ( args->gt_type==GT_NO_MISSING ) return 0; nmiss = 1; } else { if ( args->gt_type==GT_NO_HOM ) return 0; nhom = 1; } } if ( args->gt_type==GT_NEED_HOM && !nhom ) return 0; else if ( args->gt_type==GT_NEED_HET && !nhet ) return 0; else if ( args->gt_type==GT_NEED_MISSING && !nmiss ) return 0; } int minor_ac = 0; int major_ac = 0; if ( args->calc_ac ) { minor_ac = args->ac[0]; major_ac = args->ac[0]; for (i=1; in_allele; i++){ if (args->ac[i] < minor_ac) { minor_ac = args->ac[i]; } if (args->ac[i] > major_ac) { major_ac = args->ac[i]; } } } if (args->min_ac) { if (args->min_ac_type == ALLELE_NONREF && args->min_ac>non_ref_ac) return 0; // min AC else if (args->min_ac_type == ALLELE_MINOR && args->min_ac>minor_ac) return 0; // min minor AC else if (args->min_ac_type == ALLELE_ALT1 && args->min_ac>args->ac[1]) return 0; // min 1st alternate AC else if (args->min_ac_type == ALLELE_MAJOR && args->min_ac > major_ac) return 0; // min major AC else if (args->min_ac_type == ALLELE_NONMAJOR && args->min_ac > an-major_ac) return 0; // min non-major AC } if (args->max_ac) { if (args->max_ac_type == ALLELE_NONREF && args->max_acmax_ac_type == ALLELE_MINOR && args->max_acmax_ac_type == ALLELE_ALT1 && args->max_acac[1]) return 0; // max 1st alternate AC else if (args->max_ac_type == ALLELE_MAJOR && args->max_ac < major_ac) return 0; // max major AC else if (args->max_ac_type == ALLELE_NONMAJOR && args->max_ac < an-major_ac) return 0; // max non-major AC } if (args->min_af) { if (an == 0) return 0; // freq not defined, skip site if (args->min_af_type == ALLELE_NONREF && args->min_af>non_ref_ac/(double)an) return 0; // min AF else if (args->min_af_type == ALLELE_MINOR && args->min_af>minor_ac/(double)an) return 0; // min minor AF else if (args->min_af_type == ALLELE_ALT1 && args->min_af>args->ac[1]/(double)an) return 0; // min 1st alternate AF else if (args->min_af_type == ALLELE_MAJOR && args->min_af > major_ac/(double)an) return 0; // min major AF else if (args->min_af_type == ALLELE_NONMAJOR && args->min_af > (an-major_ac)/(double)an) return 0; // min non-major AF } if (args->max_af) { if (an == 0) return 0; // freq not defined, skip site if (args->max_af_type == ALLELE_NONREF && args->max_afmax_af_type == ALLELE_MINOR && args->max_afmax_af_type == ALLELE_ALT1 && args->max_afac[1]/(double)an) return 0; // max 1st alternate AF else if (args->max_af_type == ALLELE_MAJOR && args->max_af < major_ac/(double)an) return 0; // max major AF else if (args->max_af_type == ALLELE_NONMAJOR && args->max_af < (an-major_ac)/(double)an) return 0; // max non-major AF } if (args->uncalled) { if (args->uncalled == FLT_INCLUDE && an > 0) return 0; // select uncalled if (args->uncalled == FLT_EXCLUDE && an == 0) return 0; // skip if uncalled } if (args->calc_ac && args->update_info) { bcf_update_info_int32(args->hdr, line, "AC", &args->ac[1], line->n_allele-1); bcf_update_info_int32(args->hdr, line, "AN", &an, 1); } if (args->trim_alts) { int ret = bcf_trim_alleles(args->hsub ? args->hsub : args->hdr, line); if ( ret==-1 ) error("Error: some GT index is out of bounds at %s:%d\n", bcf_seqname(args->hsub ? args->hsub : args->hdr, line), line->pos+1); } if (args->phased) { int phased = bcf_all_phased(args->hdr, line); if (args->phased == FLT_INCLUDE && !phased) { return 0; } // skip unphased if (args->phased == FLT_EXCLUDE && phased) { return 0; } // skip phased } if (args->sites_only) bcf_subset(args->hsub ? args->hsub : args->hdr, line, 0, 0); return 1; } void set_allele_type (int *atype, char *atype_string) { *atype = ALLELE_NONREF; if (strcmp(atype_string, "minor") == 0) { *atype = ALLELE_MINOR; } else if (strcmp(atype_string, "alt1") == 0) { *atype = ALLELE_ALT1; } else if (strcmp(atype_string, "nref") == 0) { *atype = ALLELE_NONREF; } else if (strcmp(atype_string, "major") == 0) { *atype = ALLELE_MAJOR; } else if (strcmp(atype_string, "nonmajor") == 0) { *atype = ALLELE_NONMAJOR; } else { error("Error: allele type (%s) not recognised. Must be one of nref|alt1|minor|major|nonmajor: %s\n", atype_string); } } static void usage(args_t *args) { fprintf(stderr, "\n"); fprintf(stderr, "About: VCF/BCF conversion, view, subset and filter VCF/BCF files.\n"); fprintf(stderr, "Usage: bcftools view [options] [region1 [...]]\n"); fprintf(stderr, "\n"); fprintf(stderr, "Output options:\n"); fprintf(stderr, " -G, --drop-genotypes drop individual genotype information (after subsetting if -s option set)\n"); fprintf(stderr, " -h/H, --header-only/--no-header print the header only/suppress the header in VCF output\n"); fprintf(stderr, " -l, --compression-level [0-9] compression level: 0 uncompressed, 1 best speed, 9 best compression [%d]\n", args->clevel); fprintf(stderr, " -o, --output-file output file name [stdout]\n"); fprintf(stderr, " -O, --output-type b: compressed BCF, u: uncompressed BCF, z: compressed VCF, v: uncompressed VCF [v]\n"); fprintf(stderr, " -r, --regions restrict to comma-separated list of regions\n"); fprintf(stderr, " -R, --regions-file restrict to regions listed in a file\n"); fprintf(stderr, " -t, --targets [^] similar to -r but streams rather than index-jumps. Exclude regions with \"^\" prefix\n"); fprintf(stderr, " -T, --targets-file [^] similar to -R but streams rather than index-jumps. Exclude regions with \"^\" prefix\n"); fprintf(stderr, "\n"); fprintf(stderr, "Subset options:\n"); fprintf(stderr, " -a, --trim-alt-alleles trim alternate alleles not seen in the subset\n"); fprintf(stderr, " -I, --no-update do not (re)calculate INFO fields for the subset (currently INFO/AC and INFO/AN)\n"); fprintf(stderr, " -s, --samples [^] comma separated list of samples to include (or exclude with \"^\" prefix)\n"); fprintf(stderr, " -S, --samples-file [^] file of samples to include (or exclude with \"^\" prefix)\n"); fprintf(stderr, " --force-samples only warn about unknown subset samples\n"); fprintf(stderr, "\n"); fprintf(stderr, "Filter options:\n"); fprintf(stderr, " -c/C, --min-ac/--max-ac [:] minimum/maximum count for non-reference (nref), 1st alternate (alt1), least frequent\n"); fprintf(stderr, " (minor), most frequent (major) or sum of all but most frequent (nonmajor) alleles [nref]\n"); fprintf(stderr, " -f, --apply-filters require at least one of the listed FILTER strings (e.g. \"PASS,.\")\n"); fprintf(stderr, " -g, --genotype [^] require one or more hom/het/missing genotype or, if prefixed with \"^\", exclude sites with hom/het/missing genotypes\n"); fprintf(stderr, " -i/e, --include/--exclude select/exclude sites for which the expression is true (see man page for details)\n"); fprintf(stderr, " -k/n, --known/--novel select known/novel sites only (ID is not/is '.')\n"); fprintf(stderr, " -m/M, --min-alleles/--max-alleles minimum/maximum number of alleles listed in REF and ALT (e.g. -m2 -M2 for biallelic sites)\n"); fprintf(stderr, " -p/P, --phased/--exclude-phased select/exclude sites where all samples are phased\n"); fprintf(stderr, " -q/Q, --min-af/--max-af [:] minimum/maximum frequency for non-reference (nref), 1st alternate (alt1), least frequent\n"); fprintf(stderr, " (minor), most frequent (major) or sum of all but most frequent (nonmajor) alleles [nref]\n"); fprintf(stderr, " -u/U, --uncalled/--exclude-uncalled select/exclude sites without a called genotype\n"); fprintf(stderr, " -v/V, --types/--exclude-types select/exclude comma-separated list of variant types: snps,indels,mnps,other [null]\n"); fprintf(stderr, " -x/X, --private/--exclude-private select/exclude sites where the non-reference alleles are exclusive (private) to the subset samples\n"); fprintf(stderr, "\n"); exit(1); } int main_vcfview(int argc, char *argv[]) { int c; args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->argc = argc; args->argv = argv; args->files = bcf_sr_init(); args->clevel = -1; args->print_header = 1; args->update_info = 1; args->output_type = FT_VCF; int targets_is_file = 0, regions_is_file = 0; static struct option loptions[] = { {"genotype",1,0,'g'}, {"compression-level",1,0,'l'}, {"header-only",0,0,'h'}, {"no-header",0,0,'H'}, {"exclude",1,0,'e'}, {"include",1,0,'i'}, {"trim-alt-alleles",0,0,'a'}, {"no-update",0,0,'I'}, {"drop-genotypes",0,0,'G'}, {"private",0,0,'x'}, {"exclude-private",0,0,'X'}, {"uncalled",0,0,'u'}, {"exclude-uncalled",0,0,'U'}, {"apply-filters",1,0,'f'}, {"known",0,0,'k'}, {"novel",0,0,'n'}, {"min-alleles",1,0,'m'}, {"max-alleles",1,0,'M'}, {"samples",1,0,'s'}, {"samples-file",1,0,'S'}, {"force-samples",0,0,1}, {"output-type",1,0,'O'}, {"output-file",1,0,'o'}, {"types",1,0,'v'}, {"exclude-types",1,0,'V'}, {"targets",1,0,'t'}, {"targets-file",1,0,'T'}, {"regions",1,0,'r'}, {"regions-file",1,0,'R'}, {"min-ac",1,0,'c'}, {"max-ac",1,0,'C'}, {"min-af",1,0,'q'}, {"max-af",1,0,'Q'}, {"phased",0,0,'p'}, {"exclude-phased",0,0,'P'}, {0,0,0,0} }; char *tmp; while ((c = getopt_long(argc, argv, "l:t:T:r:R:o:O:s:S:Gf:knv:V:m:M:auUhHc:C:Ii:e:xXpPq:Q:g:",loptions,NULL)) >= 0) { char allele_type[8] = "nref"; switch (c) { case 'O': switch (optarg[0]) { case 'b': args->output_type = FT_BCF_GZ; break; case 'u': args->output_type = FT_BCF; break; case 'z': args->output_type = FT_VCF_GZ; break; case 'v': args->output_type = FT_VCF; break; default: error("The output type \"%s\" not recognised\n", optarg); }; break; case 'l': args->clevel = strtol(optarg,&tmp,10); if ( *tmp ) error("Could not parse argument: --compression-level %s\n", optarg); args->output_type |= FT_GZ; break; case 'o': args->fn_out = optarg; break; case 'H': args->print_header = 0; break; case 'h': args->header_only = 1; break; case 't': args->targets_list = optarg; break; case 'T': args->targets_list = optarg; targets_is_file = 1; break; case 'r': args->regions_list = optarg; break; case 'R': args->regions_list = optarg; regions_is_file = 1; break; case 's': args->sample_names = optarg; break; case 'S': args->sample_names = optarg; args->sample_is_file = 1; break; case 1 : args->force_samples = 1; break; case 'a': args->trim_alts = 1; args->calc_ac = 1; break; case 'I': args->update_info = 0; break; case 'G': args->sites_only = 1; break; case 'f': args->files->apply_filters = optarg; break; case 'k': args->known = 1; break; case 'n': args->novel = 1; break; case 'm': args->min_alleles = strtol(optarg,&tmp,10); if ( *tmp ) error("Could not parse argument: --min-alleles %s\n", optarg); break; case 'M': args->max_alleles = strtol(optarg,&tmp,10); if ( *tmp ) error("Could not parse argument: --max-alleles %s\n", optarg); break; case 'v': args->include_types = optarg; break; case 'V': args->exclude_types = optarg; break; case 'e': args->filter_str = optarg; args->filter_logic |= FLT_EXCLUDE; break; case 'i': args->filter_str = optarg; args->filter_logic |= FLT_INCLUDE; break; case 'c': { args->min_ac_type = ALLELE_NONREF; if ( sscanf(optarg,"%d:%s",&args->min_ac, allele_type)!=2 && sscanf(optarg,"%d",&args->min_ac)!=1 ) error("Error: Could not parse --min-ac %s\n", optarg); set_allele_type(&args->min_ac_type, allele_type); args->calc_ac = 1; break; } case 'C': { args->max_ac_type = ALLELE_NONREF; if ( sscanf(optarg,"%d:%s",&args->max_ac, allele_type)!=2 && sscanf(optarg,"%d",&args->max_ac)!=1 ) error("Error: Could not parse --max-ac %s\n", optarg); set_allele_type(&args->max_ac_type, allele_type); args->calc_ac = 1; break; } case 'q': { args->min_af_type = ALLELE_NONREF; if ( sscanf(optarg,"%f:%s",&args->min_af, allele_type)!=2 && sscanf(optarg,"%f",&args->min_af)!=1 ) error("Error: Could not parse --min_af %s\n", optarg); set_allele_type(&args->min_af_type, allele_type); args->calc_ac = 1; break; } case 'Q': { args->max_af_type = ALLELE_NONREF; if ( sscanf(optarg,"%f:%s",&args->max_af, allele_type)!=2 && sscanf(optarg,"%f",&args->max_af)!=1 ) error("Error: Could not parse --min_af %s\n", optarg); set_allele_type(&args->max_af_type, allele_type); args->calc_ac = 1; break; } case 'x': args->private_vars |= FLT_INCLUDE; args->calc_ac = 1; break; case 'X': args->private_vars |= FLT_EXCLUDE; args->calc_ac = 1; break; case 'u': args->uncalled |= FLT_INCLUDE; args->calc_ac = 1; break; case 'U': args->uncalled |= FLT_EXCLUDE; args->calc_ac = 1; break; case 'p': args->phased |= FLT_INCLUDE; break; // phased case 'P': args->phased |= FLT_EXCLUDE; break; // exclude-phased case 'g': { if ( !strcasecmp(optarg,"hom") ) args->gt_type = GT_NEED_HOM; else if ( !strcasecmp(optarg,"het") ) args->gt_type = GT_NEED_HET; else if ( !strcasecmp(optarg,"miss") ) args->gt_type = GT_NEED_MISSING; else if ( !strcasecmp(optarg,"^hom") ) args->gt_type = GT_NO_HOM; else if ( !strcasecmp(optarg,"^het") ) args->gt_type = GT_NO_HET; else if ( !strcasecmp(optarg,"^miss") ) args->gt_type = GT_NO_MISSING; else error("The argument to -g not recognised. Expected one of hom/het/^hom/^het, got \"%s\".\n", optarg); break; } case '?': usage(args); default: error("Unknown argument: %s\n", optarg); } } if ( args->filter_logic == (FLT_EXCLUDE|FLT_INCLUDE) ) error("Only one of -i or -e can be given.\n"); if ( args->private_vars > FLT_EXCLUDE ) error("Only one of -x or -X can be given.\n"); if ( args->uncalled > FLT_EXCLUDE ) error("Only one of -u or -U can be given.\n"); if ( args->phased > FLT_EXCLUDE ) error("Only one of -p or -P can be given.\n"); if ( args->sample_names && args->update_info) args->calc_ac = 1; char *fname = NULL; if ( optind>=argc ) { if ( !isatty(fileno((FILE *)stdin)) ) fname = "-"; // reading from stdin else usage(args); } else fname = argv[optind]; // read in the regions from the command line if ( args->regions_list ) { if ( bcf_sr_set_regions(args->files, args->regions_list, regions_is_file)<0 ) error("Failed to read the regions: %s\n", args->regions_list); } else if ( optind+1 < argc ) { int i; kstring_t tmp = {0,0,0}; kputs(argv[optind+1],&tmp); for (i=optind+2; ifiles, tmp.s, 0)<0 ) error("Failed to read the regions: %s\n", tmp.s); free(tmp.s); } if ( args->targets_list ) { if ( bcf_sr_set_targets(args->files, args->targets_list, targets_is_file, 0)<0 ) error("Failed to read the targets: %s\n", args->targets_list); } if ( !bcf_sr_add_reader(args->files, fname) ) error("Failed to open %s: %s\n", fname,bcf_sr_strerror(args->files->errnum)); init_data(args); bcf_hdr_t *out_hdr = args->hnull ? args->hnull : (args->hsub ? args->hsub : args->hdr); if (args->print_header) bcf_hdr_write(args->out, out_hdr); else if ( args->output_type & FT_BCF ) error("BCF output requires header, cannot proceed with -H\n"); if (!args->header_only) { while ( bcf_sr_next_line(args->files) ) { bcf1_t *line = args->files->readers[0].buffer[0]; if ( line->errcode && out_hdr!=args->hdr ) error("Undefined tags in the header, cannot proceed in the sample subset mode.\n"); if ( subset_vcf(args, line) ) bcf_write1(args->out, out_hdr, line); } } hts_close(args->out); destroy_data(args); bcf_sr_destroy(args->files); free(args); return 0; } bcftools-1.2/vcmp.c000066400000000000000000000073441246371514100143140ustar00rootroot00000000000000/* vcmp.c -- reference allele utility functions. Copyright (C) 2013 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include "vcmp.h" struct _vcmp_t { char *dref; int ndref, mdref; // ndref: positive when ref1 longer, negative when ref2 is longer int nmatch; int *map, mmap; }; vcmp_t *vcmp_init() { return (vcmp_t*)calloc(1,sizeof(vcmp_t)); } void vcmp_destroy(vcmp_t *vcmp) { free(vcmp->map); free(vcmp->dref); free(vcmp); } int vcmp_set_ref(vcmp_t *vcmp, char *ref1, char *ref2) { vcmp->ndref = 0; char *a = ref1, *b = ref2; while ( *a && *b && *a==*b ) { a++; b++; } if ( !*a && !*b ) return 0; if ( *a && *b ) return -1; // refs not compatible if ( *a ) // ref1 is longer { vcmp->nmatch = b-ref2; while ( *a ) a++; vcmp->ndref = (a-ref1) - vcmp->nmatch; hts_expand(char,vcmp->ndref+1,vcmp->mdref,vcmp->dref); memcpy(vcmp->dref,ref1+vcmp->nmatch,vcmp->ndref); vcmp->dref[vcmp->ndref] = 0; return 0; } // ref2 is longer vcmp->nmatch = a-ref1; while ( *b ) b++; vcmp->ndref = (b-ref2) - vcmp->nmatch; hts_expand(char,vcmp->ndref+1,vcmp->mdref,vcmp->dref); memcpy(vcmp->dref,ref2+vcmp->nmatch,vcmp->ndref); vcmp->dref[vcmp->ndref] = 0; vcmp->ndref *= -1; return 0; } int vcmp_find_allele(vcmp_t *vcmp, char **als1, int nals1, char *al2) { int i, j; for (i=0; indref ) { if ( !*a && !*b ) break; // found continue; } // the prefixes match if ( *a ) { if ( vcmp->ndref<0 ) continue; for (j=0; jndref; j++) if ( !a[j] || a[j]!=vcmp->dref[j] ) break; if ( j!=vcmp->ndref || a[j] ) continue; break; // found } if ( vcmp->ndref>0 ) continue; for (j=0; j<-vcmp->ndref; j++) if ( !b[j] || b[j]!=vcmp->dref[j] ) break; if ( j!=-vcmp->ndref || b[j] ) continue; break; // found } if (i==nals1) return -1; return i; } int *vcmp_map_ARvalues(vcmp_t *vcmp, int n, int nals1, char **als1, int nals2, char **als2) { if ( vcmp_set_ref(vcmp,als1[0],als2[0]) < 0 ) return NULL; vcmp->map = (int*) realloc(vcmp->map,sizeof(int)*n); int i, ifrom = n==nals2 ? 0 : 1; for (i=ifrom; imap[i-ifrom] = vcmp_find_allele(vcmp, als1+ifrom, nals1-ifrom, als2[i]); } return vcmp->map; } bcftools-1.2/vcmp.h000066400000000000000000000045231246371514100143150ustar00rootroot00000000000000/* vcmp.h -- reference allele utility functions. Copyright (C) 2013-2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #ifndef __VCMP_H__ #define __VCMP_H__ typedef struct _vcmp_t vcmp_t; vcmp_t *vcmp_init(void); void vcmp_destroy(vcmp_t *vcmp); /* * vcmp_set_ref() - sets and compares reference alleles * Returns 0 on success or -1 if alleles not compatible */ int vcmp_set_ref(vcmp_t *vcmp, char *ref1, char *ref2); /* * vcmp_find_allele() * @param als1: alternate alleles to ref1 above * @param al2: alternate allele to ref2 above * Returns -1 if not found or 0-based index to als1 of matching allele */ int vcmp_find_allele(vcmp_t *vcmp, char **als1, int nals1, char *al2); /* * vcmp_map_ARvalues() - Create mapping for Number=A,R tag values * @param number: nals2 for Number=R, nals2-1 for Number=A * @param nals1: number of alleles * @param als1: alleles * * Returns pointer to an array of size nals2 with mapping from als2 * to als1 or NULL if REFs (als1[0] and als2[0]) are not compatible. * If i is the index of an allele in als2, ret[i] is the index of matching * allele in als1 or -1 if als2 does not have a matching allele. * The caller must not free the array. */ int *vcmp_map_ARvalues(vcmp_t *vcmp, int number, int nals1, char **als1, int nals2, char **als2); #endif bcftools-1.2/version.c000066400000000000000000000031041246371514100150220ustar00rootroot00000000000000/* version.c -- report version numbers for plugins. Copyright (C) 2014 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include "bcftools.h" #include "version.h" void version(const char **bcftools_version, const char **htslib_version) { *bcftools_version = BCFTOOLS_VERSION; *htslib_version = hts_version(); } void error(const char *format, ...) { va_list ap; va_start(ap, format); vfprintf(stderr, format, ap); va_end(ap); exit(-1); }